Michael D Sacco, Lauren R Hammond, Radwan E Noor, Dipanwita Bhattacharya, Lily J McKnight, Jesper J Madsen, Xiujun Zhang, Shane G Butler, M Trent Kemp, Aiden C Jaskolka-Brown, Sebastian J Khan, Ioannis Gelis, Prahathees Eswara, Yu Chen
In the Firmicutes phylum, GpsB is a membrane associated protein that coordinates peptidoglycan synthesis with cell growth and division. Although GpsB has been studied in several bacteria, the structure, function, and interactome of Staphylococcus aureus GpsB is largely uncharacterized. To address this knowledge gap, we solved the crystal structure of the N-terminal domain of S. aureus GpsB, which adopts an atypical, asymmetric dimer, and demonstrates major conformational flexibility that can be mapped to a hinge region formed by a three-residue insertion exclusive to Staphylococci ...
April 19, 2024: ELife