keyword
Keywords Extraction and purification of...

Extraction and purification of membrane proteins

https://read.qxmd.com/read/37643406/accurate-and-convenient-lung-cancer-diagnosis-through-detection-of-extracellular-vesicle-membrane-proteins-via-f%C3%A3-rster-resonance-energy-transfer
#21
JOURNAL ARTICLE
Shuting Xiao, Yi Yao, Shuilin Liao, Bin Xu, Xue Li, Yuxiao Zhang, Lei Zhang, Qiang Chen, Haoneng Tang, Qibin Song, Ming Dong
Tumor-derived extracellular vesicles (EVs) are promising to monitor early stage cancer. Unfortunately, isolating and analyzing EVs from a patient's liquid biopsy are challenging. For this, we devised an EV membrane proteins detection system (EV-MPDS) based on Förster resonance energy transfer (FRET) signals between aptamer quantum dots and AIEgen dye, which eliminated the EV extraction and purification to conveniently diagnose lung cancer. In a cohort of 80 clinical samples, this system showed enhanced accuracy (100% versus 65%) and sensitivity (100% versus 55%) in cancer diagnosis as compared to the ELISA detection method...
August 29, 2023: Nano Letters
https://read.qxmd.com/read/37623384/isolation-and-identification-of-antioxidant-peptides-derived-from-cricket-gryllus-bimaculatus-protein-fractions
#22
JOURNAL ARTICLE
Olumide Oluwatoyosi Fashakin, Pipat Tangjaidee, Kridsada Unban, Wannaporn Klangpetch, Tabkrich Khumsap, Korawan Sringarm, Saroat Rawdkuen, Suphat Phongthai
Crickets contain high protein content that can be used to improve nutrition but are less exploited. This study was conducted to isolate different Cricket Protein Fractions including albumin, globulin, glutelin, and prolamin. All fractions were characterized and hydrolyzed by commercial enzymes. The results showed that the glutelin fractions had the highest extraction yields with 53.9 ± 2.12% ( p < 0.05). Moreover, glutelin hydrolysate fraction prepared by Alcalase with a 16.35 ±0.29% hydrolysis degree was selected for further purification because of their high antioxidant activities, including ABTS radical-scavenging activity (0...
July 29, 2023: Insects
https://read.qxmd.com/read/37575399/enrichment-of-membrane-proteins-for-downstream-analysis-using-styrene-maleic-acid-lipid-particles-smalps-extraction
#23
JOURNAL ARTICLE
Benedict Dirnberger, Dagmara Korona, Rebeka Popovic, Michael J Deery, Helen Barber, Steven Russell, Kathryn S Lilley
Integral membrane proteins are an important class of cellular proteins. These take part in key cellular processes such as signaling transducing receptors to transporters, many operating within the plasma membrane. More than half of the FDA-approved protein-targeting drugs operate via interaction with proteins that contain at least one membrane-spanning region, yet the characterization and study of their native interactions with therapeutic agents remains a significant challenge. This challenge is due in part to such proteins often being present in small quantities within a cell...
August 5, 2023: Bio-protocol
https://read.qxmd.com/read/37541256/genetically-incorporated-crosslinkers-identify-regulators-of-membrane-protein-pd-l1-in-mammalian-cells
#24
JOURNAL ARTICLE
Fangni Chai, Pan Li, Yong He, Zhihui Zhou, Shupan Guo, Xin Liu, Li Zhou, Haiyan Ren
Profiling membrane proteins' interacting networks is crucial for understanding their regulatory mechanisms and functional characteristics, but it remains a challenging task. Here, by combining genetic incorporation of crosslinkers, tandem denatured purification, and proteomics, we added interaction partners for PD-L1, a cancer cell surface protein that inhibits T cell activity. The site-specifically incorporated crosslinker mediates the covalent capture of interactions under physiological conditions and enabled the PD-L1 complexes to withstand the harsh extraction conditions of membrane proteins...
July 29, 2023: Cell Chemical Biology
https://read.qxmd.com/read/37538180/inhibitory-activity-of-flavonoids-fraction-from-astragalus-membranaceus-fisch-ex-bunge-stems-and-leaves-on-bacillus-cereus-and-its-separation-and-purification
#25
JOURNAL ARTICLE
Liyan Cui, Zhennan Ma, Wenhui Li, Haihui Ma, Shang Guo, Defu Wang, Yanbing Niu
Introduction: Astragalus membranaceus Fisch. ex Bunge is a traditional botanical drug with antibacterial, antioxidant, antiviral, and other biological activities. In the process of industrialization of A. membranaceus , most of the aboveground stems and leaves are discarded without resource utilization except for a small amount of low-value applications such as composting. This study explored the antibacterial activity of A. membranaceus stem and leaf extracts to evaluate its potential as a feed antibiotic substitute...
2023: Frontiers in Pharmacology
https://read.qxmd.com/read/37441251/plant-derived-exosomal-nanoparticles-potential-therapeutic-for-inflammatory-bowel-disease
#26
REVIEW
De-Feng Li, Qi Tang, Mei-Feng Yang, Hao-Ming Xu, Min-Zheng Zhu, Yuan Zhang, Cheng-Mei Tian, Yu-Qiang Nie, Jian-Yao Wang, Yu-Jie Liang, Li-Sheng Wang, Jun Yao
Inflammatory bowel disease (IBD), encompassing Crohn's disease and ulcerative colitis, is a chronic autoimmune disorder characterized by inflammation. However, currently available disease-modifying anti-IBD drugs exhibit limited efficacy in IBD therapy. Furthermore, existing therapeutic approaches provide only partial relief from IBD symptoms and are associated with certain side effects. In recent years, a novel category of nanoscale membrane vesicles, known as plant-derived exosome-like nanoparticles (PDENs), has been identified in edible plants...
July 11, 2023: Nanoscale advances
https://read.qxmd.com/read/37397795/purification-of-recombinant-human-amphiphysin-1-and-its-n-bar-domain
#27
JOURNAL ARTICLE
Samsuzzoha Mondal, Honey Priya James, Francesco Milano, Rui Jin, Tobias Baumgart
Bin/Amphiphysin/Rvs (BAR) proteins are known as classical membrane curvature generators during endocytosis. Amphiphysin, a member of the N-BAR sub-family of proteins that contain a characteristic amphipathic sequence at the N-terminus of the BAR domain, is involved in clathrin-mediated endocytosis. Full-length amphiphysin contains a ~ 400 amino acid long disordered linker connecting the N-BAR domain and a C-terminal Src homology 3 (SH3) domain. We express and purify recombinant amphiphysin and its N-BAR domain along with an N-terminal glutathione-S-transferase (GST) tag...
June 20, 2023: Bio-protocol
https://read.qxmd.com/read/37353118/inulinase-and-fructooligosaccharide-production-from-carob-using-aspergillus-niger-a42-atcc-204447-under-solid-state-fermentation-conditions
#28
JOURNAL ARTICLE
Muge Canatar, Hilal Nur Gürler Tufan, Selime Benemir Erkan Ünsal, Cansu Yılmazer Koc, Ali Ozcan, Gokce Kucuk, Selin Basmak, Ercan Yatmaz, Mustafa Germec, Ibrahim Yavuz, Irfan Turhan
This study aimed to the production of inulinase and fructooligosaccharides (FOSs) from carob under the solid-state fermentation (SSF) conditions by using Plackett-Burman Design (PBD). Based on the results the maximum inulinase and specific inulinase activities were 249.98 U/mL and 318.29 U/mg protein, respectively. When the fructooligosaccharide (FOS) results were evaluated, the maximum values of 1,1,1-Kestopentaose, 1,1-Kestotetraose, and 1-Kestose were 182.01, 506.16, 132.16 ppm while the lowest and highest total FOS values were 179...
June 21, 2023: International Journal of Biological Macromolecules
https://read.qxmd.com/read/37324655/different-culture-media-and-purification-methods-unveil-the-core-proteome-of-propionibacterium-freudenreichii-derived-extracellular-vesicles
#29
JOURNAL ARTICLE
Vinícius de Rezende Rodovalho, Brenda Silva Rosa da Luz, Aurélie Nicolas, Julien Jardin, Valérie Briard-Bion, Edson Luiz Folador, Anderson Rodrigues Santos, Gwénaël Jan, Yves Le Loir, Vasco Ariston de Carvalho Azevedo, Éric Guédon
Bacterial extracellular vesicles (EVs) are natural lipidic nanoparticles implicated in intercellular communication. Although EV research focused mainly on pathogens, the interest in probiotic-derived EVs is now rising. One example is Propionibacterium freudenreichii , which produces EVs with anti-inflammatory effects on human epithelial cells. Our previous study with P. freudenreichii showed that EVs purified by size exclusion chromatography (SEC) displayed variations in protein content according to bacterial growth conditions...
2023: Microlife
https://read.qxmd.com/read/37324654/evaluation-of-the-antibacterial-and-antibiofilm-effects-of-ethyl-acetate-root-extracts-from-vernonia-adoensis-asteraceae-against-pseudomonas-aeruginosa
#30
JOURNAL ARTICLE
Mercy Masuku, Winnie Mozirandi, Stanley Mukanganyama
There is an increase in mortality and morbidity in the health facilities due to nosocomial infections caused by multidrug-resistant nosocomial bacteria; hence, there is a need for new antibacterial agents. Vernonia adoensis has been found to possess medicinal value. Plant phytochemicals may have antimicrobial activity against some resistant pathogens. The antibacterial efficacy of root extracts against Staphylococcus aureus and Pseudomonas aeruginosa was investigated using the microbroth dilution method. All extracts from the roots had an inhibitory effect on the growth of both bacteria, with the most susceptible being P...
2023: TheScientificWorldJournal
https://read.qxmd.com/read/37292027/3d-printed-integrated-nanoporous-membranes-for-electroextraction-of-dna
#31
JOURNAL ARTICLE
Hari Kalathil Balakrishnan, Soo Min Lee, Ludovic F Dumée, Egan H Doeven, Richard Alexander, Dan Yuan, Rosanne M Guijt
3D printing is established as an alternative microfabrication approach, and while printer resolution limits the direct 3D printing of pore features in the micron/submicron range, the use of nanoporous materials allows for the integration of porous membranes in 3D printed devices. Here, nanoporous membranes were formed by digital light projection (DLP) 3D printing using a polymerization-induced phase separation (PIPS) resin formulation. A functionally integrated device was fabricated using resin exchange following a simple, semi-automated manufacturing process...
June 9, 2023: Nanoscale
https://read.qxmd.com/read/37263243/rationale-for-adsorption-in-extracorporeal-blood-purification
#32
REVIEW
Thiago Reis, Fábio Reis, Antônio Fagundes, Rogério da Hora Passos, Francisco de Assis Rocha Neves
A strong rationale supports the development of adsorption-based extracorporeal blood purification in conditions such as sepsis, acute kidney disease, uremia, and acute liver failure. The retention of compounds as a consequence of acute or chronic organ dysfunction might have detrimental effects. When a causative effect of an accumulated compound in a pathogenic condition is demonstrated, a rationale for the removal of this solute is also established. Adsorption is a mass transfer mechanism in which a solute chemically interacts with the surface of a solid structure (sorbent) and is removed from its solvent (i...
2023: Contributions to Nephrology
https://read.qxmd.com/read/37255444/conformations-of-human-immunodeficiency-virus-envelope-glycoproteins-in-detergents-and-styrene-maleic-acid-lipid-particles
#33
JOURNAL ARTICLE
Rong Zhou, Shijian Zhang, Hanh T Nguyen, Haitao Ding, Althea Gaffney, John C Kappes, Amos B Smith, Joseph G Sodroski
The mature human immunodeficiency virus (HIV) envelope glycoprotein (Env) trimer, which consists of noncovalently associated gp120 exterior and gp41 transmembrane subunits, mediates virus entry into cells. The pretriggered (State-1) Env conformation is the major target for broadly neutralizing antibodies (bNAbs), whereas receptor-induced downstream Env conformations elicit immunodominant, poorly neutralizing antibody (pNAb) responses. To examine the contribution of membrane anchorage to the maintenance of the metastable pretriggered Env conformation, we compared wild-type and State-1-stabilized Envs solubilized in detergents or in styrene-maleic acid (SMA) copolymers...
May 31, 2023: Journal of Virology
https://read.qxmd.com/read/37219402/proteomic-tools-to-study-phosphorylation-of-intrinsically-disordered-proteins
#34
REVIEW
Barbara Spolaore, Luca Secco, Giulia Rocca, Guidalberto Manfioletti, Giorgio Arrigoni, Riccardo Sgarra
INTRODUCTION: Intrinsically disordered proteins (IDPs) represent a family of proteins that lacks secondary or tertiary structure. IDPs are hubs in interaction networks, participate in liquid-liquid phase separation processes, and drive the formation of proteinaceous membrane-less organelles. Their unfolded structure makes them particularly prone to post-translational modifications (PTMs) that play key functional modulatory roles. AREAS COVERED: We discuss different analytical approaches to study phosphorylation of IDPs starting from methods for IDPs enrichment (strong acid extractions and heat-based pre-fractionation), strategies to enrich and map phosphopeptides/proteins, and mass spectrometry-based tools to study the phosphorylation-dependent conformational alterations of IDPs (limited proteolysis, HDX, chemical cross-linking, covalent labelling, and ion mobility)...
May 23, 2023: Expert Review of Proteomics
https://read.qxmd.com/read/37211149/plant-cellulose-synthase-membrane-protein-isolation-directly-from-pichia-pastoris-protoplasts-liposome-reconstitution-and-its-enzymatic-characterization
#35
JOURNAL ARTICLE
Dharanidaran Jayachandran, Shoili Banerjee, Shishir P S Chundawat
Cellulose is synthesized by a plant cell membrane-integrated processive glycosyltransferase (GT) called cellulose synthase (CesA). Since only a few of these plant CesAs have been purified and characterized to date, there are huge gaps in our mechanistic understanding of these enzymes. The biochemistry and structural biology studies of CesAs are currently hampered by challenges associated with their expression and extraction at high yields. To aid in understanding CesA reaction mechanisms and to provide a more efficient CesA extraction method, two putative plant CesAs - PpCesA5 from Physcomitrella patens and PttCesA8 from Populus tremula x tremuloides that are involved in primary and secondary cell wall formation in plants were expressed using Pichia pastoris as an expression host...
May 19, 2023: Protein Expression and Purification
https://read.qxmd.com/read/37172458/effects-of-4-tert-butylpyrocatechol-and-tea-polyphenol-on-growth-physiology-and-antioxidant-responses-in-microcystis-aeruginosa
#36
JOURNAL ARTICLE
Yitong Lu, Xinxin Jiang, Hongzhou Xu, Chengrong Liu, Yanzhen Song, Kuiquan Pan, Lixin Wang, Liang Du, Haixia Liu
Global warming has increased the frequency of Microcystis aeruginosa blooms, leading to the deterioration of water quality and loss of biodiversity. Therefore, developing effective strategies for controlling M. aeruginosa blooms has become an important research topic. Plant extracts, 4‑tert-butylpyrocatechol (TBC) and tea polyphenol (TP) are commonly used for water purification and to increase fish immunity, which have great potential to inhibit cyanobacterial blooms. The inhibitory effects of TBC and TP on M...
April 14, 2023: Aquatic Toxicology
https://read.qxmd.com/read/36999703/bromelain-an-enzyme-expanding-its-horizon-from-food-to-pharmaceutical-industry
#37
JOURNAL ARTICLE
Gauresh Sharma, Archana Vimal
Bromelain is a protein digestive enzyme obtained from the extract of pineapple (steam, fruit, and leaves). It is a cocktail of several thiol endopeptidases and other components like peroxidase, cellulase, phosphatase, and several protease inhibitors. It is a glycoprotein with an oligosaccharide in its molecular structure that contains xylose, fucose, mannose, and N-acetyl glucosamine. Many approaches have been used in the extraction and purification of bromelain like filtration, membrane filtration, INT filtration, precipitation, aqueous two-phase system, ion-exchange chromatography, etc...
March 31, 2023: Current Pharmaceutical Biotechnology
https://read.qxmd.com/read/36997896/increased-synovial-immunohistochemistry-reactivity-of-tgf-%C3%AE-1-in-erosive-peripheral-psoriatic-arthritis
#38
JOURNAL ARTICLE
Jose A Pinto Tasende, M Fernandez-Moreno, M E Vazquez-Mosquera, J C Fernandez-Lopez, N Oreiro-Villar, F J De Toro Santos, F J Blanco-García
BACKGROUND: Immune and non-immune cells contribute to the pathology of chronic arthritis, and they can contribute to tissue remodeling and repair as well as disease pathogenesis. The present research aimed to analyze inflammation and bone destruction/regeneration biomarkers in patients with psoriatic arthritis (PsA), rheumatoid arthritis (RA), osteoarthritis (OA), and ankylosing spondylitis (AS). METHODS: Samples were obtained from the inflamed knee of patients with knee arthritis who had been referred for undergoing arthroscopies...
March 30, 2023: BMC Musculoskeletal Disorders
https://read.qxmd.com/read/36934326/protocol-to-test-the-utility-of-detergents-for-e-%C3%A2-coli-membrane-protein-extraction-and-delipidation
#39
JOURNAL ARTICLE
Virginia Wycisk, Leonhard H Urner
We present a protocol to evaluate the utility of detergents for purification and delipidation of E. coli membrane proteins. We determine the critical aggregation concentration of detergents. Furthermore, we compare the ability of detergents to extract membrane proteins and to maintain protein-lipid interactions during purification. The protocol describes steps for isolating and delipidating membrane proteins from E. coli membranes by extraction and affinity purification using detergents. The protocol does not enable an absolute quantification of purification outcomes...
March 17, 2023: STAR protocols
https://read.qxmd.com/read/36933298/development-of-molecular-and-antigenic-based-rapid-tests-for-the-identification-of-african-swine-fever-virus-in-different-tissues
#40
JOURNAL ARTICLE
Simone Cavalera, Barbara Colitti, Gian Mario De Mia, Francesco Feliziani, Silvia Dei Giudici, Pier Paolo Angioi, Federica D'Errico, Daniela Scalas, Annalisa Scollo, Thea Serra, Matteo Chiarello, Valentina Testa, Fabio Di Nardo, Claudio Baggiani, Annalisa Oggiano, Sergio Rosati, Laura Anfossi
African swine fever (ASF) is a severe haemorrhagic infectious disease affecting suids, thus representing a great economic concern. Considering the importance of the early diagnosis, rapid point of care testing (POCT) for ASF is highly demanded. In this work, we developed two strategies for the rapid onsite diagnosis of ASF, based on Lateral Flow Immunoassay (LFIA) and Recombinase Polymerase Amplification (RPA) techniques. The LFIA was a sandwich-type immunoassay exploiting a monoclonal antibody directed towards the p30 protein of the virus (Mab)...
March 14, 2023: Talanta
keyword
keyword
58871
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.