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https://www.readbyqxmd.com/read/28930183/substituting-sodium-hydrosulfite-with-sodium-metabisulfite-improves-long-term-stability-of-a-distributable-paper-based-test-kit-for-point-of-care-screening-for-sickle-cell-anemia
#1
Kian Torabian, Dalia Lezzar, Nathaniel Z Piety, Alex George, Sergey S Shevkoplyas
Sickle cell anemia (SCA) is a genetic blood disorder that is particularly lethal in early childhood. Universal newborn screening programs and subsequent early treatment are known to drastically reduce under-five SCA mortality. However, in resource-limited settings, cost and infrastructure constraints limit the effectiveness of laboratory-based SCA screening programs. To address this limitation our laboratory previously developed a low-cost, equipment-free, point-of-care, paper-based SCA test. Here, we improved the stability and performance of the test by replacing sodium hydrosulfite (HS), a key reducing agent in the hemoglobin solubility buffer which is not stable in aqueous solutions, with sodium metabisulfite (MS)...
September 20, 2017: Biosensors
https://www.readbyqxmd.com/read/28926139/the-synonymous-nucleotide-substitution-rhd-1056c-g-alters-mrna-splicing-associated-with-serologically-weak-d-phenotype
#2
Sejong Chun, Jae Won Yun, Geon Park, Duck Cho
BACKGROUND: D antigen is one of the most clinically significant blood group antigens. Variation of the RHD gene can cause weak D or partial D phenotypes. While most variations are missense substitutions with amino acid changes, those without are called "silent" or "synonymous" substitutions. Synonymous substitutions often have little effect on the protein, not altering the phenotype. However, effect on splicing can affect end-product protein. We report a new synonymous variation, RHD 1056C>G, that resulted in weak D phenotype, and predicted its effect with various in silico methods...
September 19, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28923568/a-method-for-determining-polysaccharide-content-in-biological-samples
#3
Duoduo Xu, Wei Zheng, Yanqiu Zhang, Qipin Gao, Mingxing Wang, Yang Gao
Difficulties in the determination of polysaccharide content in biological samples has been one of the bottlenecks limiting the development of polysaccharides pharmacokinetics, greatly due to the complicated chemical structure of these compounds. In this study, we established a simple, reproducible and reliable method for the determination of polysaccharide content in biological samples. Polysaccharides were replaced by fluorescein isothiocyanate (FITC) and iodine to generate a complex. The iodine content in the complex was measured by inductively coupled plasma-mass spectrometry (ICP-MS) to indirectly reflect polysaccharide content...
September 15, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28918917/leukocytapheresis-in-rheumatoid-arthritis
#4
REVIEW
Toshihiko Hidaka, Yayoi Hashiba, Kazuyoshi Kubo, Yasufumi Kai, Keiichi Maeda
In this article, we discussed leukocytapheresis (LCAP) for rheumatoid arthritis (RA). Recently, a simple and practical on-line continuous LACP system has been developed. It is equipped with a direct hemoperfusion column (Cellsorba(®), Asahikasei Medical Co., Ltd.) packed with fine-diameter polyester fibers, which are commonly used to adsorb white blood cells to prevent a graft-versus-host reaction during blood transfusion. Clinical trials revealed that LCAP is a effective and safe therapy for patients with drug-resistant RA or RA complicated with vasculitis...
August 30, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28917801/development-of-a-quantitative-approach-in-blood-plasma-for-low-dosed-hallucinogens-and-opioids-using-lc-high-resolution-mass-spectrometry
#5
Achim T Caspar, Andreas B Kollas, Hans H Maurer, Markus R Meyer
The WHO annually reports an increasing abuse of new psychoactive substances (NPS), which are a heterogeneous group of synthetic drugs and are consumed as substitute for controlled drugs of abuse. In this work, we focused on highly potent derivatives such those of phenethylamine (2C), N-2-methoxybenzyl phenethylamine (NBOMes), lysergic acid diethylamide (LSD), and fentanyl. Severe to fatal intoxications were described due to their high potency. Therefore, they have to be taken at very low doses resulting in low blood concentration in the low ng/mL range, which is a challenge for reliable analytical detection and quantification...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917450/a-novel-class-of-plasmodial-clpp-protease-inhibitors-as-potential-antimalarial-agents
#6
Sourabh Mundra, Vandana Thakur, Angelica M Bello, Sumit Rathore, Mohd Asad, Lianhu Wei, Jane Yang, Sai Kumar Chakka, Radhakrishnan Mahesh, Pawan Malhotra, Asif Mohmmed, Lakshmi P Kotra
The prokaryotic ATP-dependent ClpP protease, localized in the relict plastid of malaria parasite, represents a potential drug target. In the present study, we utilized in silico structure-based screening and medicinal chemistry approaches to identify a novel pyrimidine series of compounds inhibiting P. falciparum ClpP protease activity and evaluated their antiparasitic activities. Structure-activity relationship indicated that morpholine moiety at C2, an aromatic substitution at N3 and a 4-oxo moiety on the pyrimidine are important for potent inhibition of ClpP enzyme along with antiparasiticidal activity...
September 5, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28914400/an-in-vivo-study-on-endothelialized-vascular-grafts-produced-by-autologous-biotubes-and-adipose-stem-cells-adscs
#7
Yu Chieh Tseng, Jun Neng Roan, Ying Chiang Ho, Chih Chan Lin, Ming Long Yeh
Currently, commercial synthetic vascular grafts made from Dacron and ePTFE for small-diameter, vascular applications (<6 mm) show limited reendothelization and are less compliant, often resulting in thrombosis and intimal hyperplasia. Although good blood compatibility can be achieved in autologous arteries and veins, the number of high quality harvest sites is limited, and the grafts are size-mismatched for use in the fistula or cardiovascular bypass surgery; thus, alternative small graft substitutes must be developed...
September 15, 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28910796/modeling-of-internal-filtration-in-theranova-hemodialyzers
#8
Anna Lorenzin, Mauro Neri, William R Clark, Francesco Garzotto, Alessandra Brendolan, Federico Nalesso, Nicola Marchionna, Monica Zanella, Marco Sartori, Gianfranco B Fiore, Claudio Ronco
High retention onset (HRO) is the designation for a new class of hemodialysis membranes. A unique characteristic of this class is the highly selective and controlled porosity resulting in sieving properties that provide a clinically desirable balance between middle/large molecular weight solute removal and albumin loss. Another defining feature of this membrane class is the relatively small fiber diameter, which produces high convective volumes in the form of internal filtration. The aim of the present study was to estimate, by semi-empirical methods, convective volumes for 2 new HRO dialyzers: Theranova 400 and Theranova 500 (Baxter International Inc...
2017: Contributions to Nephrology
https://www.readbyqxmd.com/read/28910401/strontium-doped-hydroxyapatite-polysaccharide-materials-effect-on-ectopic-bone-formation
#9
C Ehret, R Aid-Launais, T Sagardoy, R Siadous, R Bareille, S Rey, S Pechev, L Etienne, J Kalisky, E de Mones, D Letourneur, J Amedee Vilamitjana
Previous studies performed using polysaccharide-based matrices supplemented with hydroxyapatite (HA) particles showed their ability to form in subcutaneous and intramuscular sites a mineralized and osteoid tissue. Our objectives are to optimize the HA content in the matrix and to test the combination of HA with strontium (Sr-HA) to increase the matrix bioactivity. First, non-doped Sr-HA powders were combined to the matrix at three different ratios and were implanted subcutaneously for 2 and 4 weeks. Interestingly, matrices showed radiolucent properties before implantation...
2017: PloS One
https://www.readbyqxmd.com/read/28910078/bifacial-biomimetic-freestanding-anisotropic-catechol-interfaces-with-asymmetrically-layered-films-as-versatile-extracellular-matrix-substitutes
#10
Byung Gee Im, Minjae Do, Yoojin Kim, Mira Cho, Jae-Hyung Jang
Biological naïve extracellular matrices (ECMs) exhibit anisotropic functions in their physical, chemical, and morphological properties. Representative examples include anisotropic skin layers or blood vessels simultaneously facing multiphasic environments. Here, anisotropically multifunctional structures called BiFACIAL (biomimetic freestanding anisotropic catechol-interfaces with asymmetrically layered) films were developed simply by contacting two polysaccharide solutions of heparin-catechol (Hep-C) and chitosan-catechol (Chi-C)...
September 14, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28906021/primary-hyperparathyroidism-dynamic-postoperative-metabolic-changes
#11
Reto Martin Kaderli, Philipp Riss, Angelika Geroldinger, Andreas Selberherr, Christian Scheuba, Bruno Niederle
OBJECTIVE: Little is known about the natural changes in parathyroid function after successful parathyroid surgery for primary hyperparathyroidism. The association of intact parathyroid hormone (iPTH) and calcium (Ca) with "temporary hypoparathyroidism" and "hungry bone syndrome" (HBS) was evaluated. DESIGN: Potential risk factors for temporary hypoparathyroidism and HBS were evaluated by taking blood samples before surgery, intraoperatively, at post-operative day (POD) 1, at POD 5 to 7, in postoperative week (POW) 8 and in postoperative month (POM) 6...
September 14, 2017: Clinical Endocrinology
https://www.readbyqxmd.com/read/28904872/treatment-of-renal-anemia-erythropoiesis-stimulating-agents-and-beyond
#12
REVIEW
Patrick Biggar, Gheun-Ho Kim
Anemia, complicating the course of chronic kidney disease, is a significant parameter, whether interpreted as subjective impairment or an objective prognostic marker. Renal anemia is predominantly due to relative erythropoietin (EPO) deficiency. EPO inhibits apoptosis of erythrocyte precursors. Studies using EPO substitution have shown that increasing hemoglobin (Hb) levels up to 10-11 g/dL is associated with clinical improvement. However, it has not been unequivocally proven that further intensification of erythropoiesis stimulating agent (ESA) therapy actually leads to a comprehensive benefit for the patient, especially as ESAs are potentially associated with increased cerebro-cardiovascular events...
September 2017: Kidney Research and Clinical Practice
https://www.readbyqxmd.com/read/28904343/engineered-factor-xa-variants-retain-procoagulant-activity-independent-of-direct-factor-xa-inhibitors
#13
Daniël Verhoef, Koen M Visscher, C Ruben Vosmeer, Ka Lei Cheung, Pieter H Reitsma, Daan P Geerke, Mettine H A Bos
The absence of an adequate reversal strategy to prevent and stop potential life-threatening bleeding complications is a major drawback to the clinical use of the direct oral inhibitors of blood coagulation factor Xa. Here we show that specific modifications of the substrate-binding aromatic S4 subpocket within the factor Xa active site disrupt high-affinity engagement of the direct factor Xa inhibitors. These modifications either entail amino-acid substitution of S4 subsite residues Tyr99 and/or Phe174 (chymotrypsinogen numbering), or extension of the 99-loop that borders the S4 subsite...
September 13, 2017: Nature Communications
https://www.readbyqxmd.com/read/28895214/highly-k-selective-fluorescent-probes-for-lifetime-sensing-of-k-in-living-cells
#14
Hans-Jürgen Holdt, Thomas Schwarze, Monique Mertens, Peter Müller, Janine Riemer, Pablo Wessig
The new K+ selective fluorescent probes 1 and 2 were obtained by CuAAC reactions of an alkyne substituted DBD ester fluorophore with azido functionalized N-phenylaza-18-crown-6 ether and N-(o-isopropoxy)-phenylaza-18-crown-6 ether, respectively. Probes 1 and 2 allow the detection of K+ in the presence of Na+ in water by fluorescence enhancement (2.2 for 1 at 2000 mM K+ and 2.5 for 2 at 160 mM K+). Fluorescence life time measurements in the absence and presence of K+ revealed bi-exponential decay kinetics with similar life times, however with different proportions changing the averaged fluorescence decay times (τf(av))...
September 12, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28894710/incidence-of-medication-discrepancies-and-its-predicting-factors-in-emergency-department
#15
Morvarid Zarif-Yeganeh, Mansoor Rastegarpanah, Gholamreza Garmaroudi, Molouk Hadjibabaie, Hojjat Sheikh Motahar Vahedi
BACKGROUND: This study was conducted to evaluate the incidence of medication discrepancies and its related factors using medication reconciliation method in patients admitted to the emergency department of Tehran University of Medical Sciences hospitals. METHODS: In this cross-sectional study, 200 adult patients with at least one chronic disease that used two regular prescription medications were included in 2015. After 24 h of admission, demographic data and patient's home medications were collected...
August 2017: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/28888682/rapid-capillary-electrophoresis-approach-for-the-quantification-of-ewe-milk-adulteration-with-cow-milk
#16
Francesca Trimboli, Valeria Maria Morittu, Caterina Cicino, Camillo Palmieri, Domenico Britti
The substitution of ewe milk with more economic cow milk is a common fraud. Here we present a capillary electrophoresis method for the quantification of ewe milk in ovine/bovine milk mixtures, which allows for the rapid and inexpensive recognition of ewe milk adulteration with cow milk. We utilized a routine CE method for human blood and urine proteins analysis, which fulfilled the separation of skimmed milk proteins in alkaline buffer. Under this condition, ovine and bovine milk exhibited a recognizable and distinct CE protein profiles, with a specific ewe peak showing a reproducible migration zone in ovine/bovine mixtures...
August 30, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28880541/selenomethionine-as-a-safer-substitute-for-barium-selenate-in-long-acting-injectable-se-supplements-for-food-producing-animals
#17
Scott O Knowles, Neville D Grace
Nutritional supplementation with selenium (Se) can prevent Se deficiency in food-producing animals. Injection with slow-release formulations is a preferred method for free-range grazing sheep and cattle, and barium selenate (BaSeO4) provides optimal efficacy. This chemical can become a health risk to humans if the concentrated depot of an injection site is consumed, and consequently such use is recently banned in the EU. A possible replacement is selenomethionine (SeMet), a naturally occurring form of Se supplementation hitherto only administered orally...
September 7, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28872401/substituting-sedentary-time-with-light-and-moderate-to-vigorous-physical-activity-is-associated-with-better-cardio-metabolic-health
#18
Sara Knaeps, Stijn De Baere, Jan Bourgois, Evelien Mertens, Ruben Charlier, Johan Lefevre
BACKGROUND: Apply a more novel approach to systematically examine (1) associations of clustered cardio-metabolic risk and cardio-metabolic risk factors, and (2) theoretical substitution of sedentary time with either sleep, light physical activity (LPA) or moderate-and-vigorous physical activity (MVPA) and substituting LPA with MVPA. METHODS: Physical activity and sleep were objectively measured in 410 Flemish adults (55.5[±9.6] years, 64% men) with a SenseWear Pro 3 Armband...
September 5, 2017: Journal of Physical Activity & Health
https://www.readbyqxmd.com/read/28870472/effects-of-high-%C3%AE-glucan-barley-on-visceral-fat-obesity-in-japanese-individuals-a-randomized-double-blind-study
#19
Seiichiro Aoe, Yasunori Ichinose, Noriko Kohyama, Kozo Komae, Asuka Takahashi, Daigo Abe, Toji Yoshioka, Takashi Yanagisawa
OBJECTIVE: The aim of this study was to investigate whether a diet in which high β-glucan barley was substituted for rice would reduce visceral fat obesity in Japanese individuals. METHODS: A randomized, double-blind, placebo-controlled intervention study was conducted with 100 Japanese individuals with waist circumference (WC) ≥85 cm for men or ≥90 cm for women and body mass index (BMI) ≥24 kg/m(2). Participants were randomly assigned to consume a mixture of rice and either high β-glucan barley (test group, 4...
October 2017: Nutrition
https://www.readbyqxmd.com/read/28864250/a-straightforward-approach-to-enhance-the-textural-mechanical-and-biological-properties-of-injectable-calcium-phosphate-apatitic-cements-cpcs-cpc-blood-composites-a-comprehensive-study
#20
Charlotte Mellier, François-Xavier Lefèvre, Franck Fayon, Valérie Montouillout, Christelle Despas, Myriam Le Ferrec, Florian Boukhechba, Alain Walcarius, Pascal Janvier, Maeva Dutilleul, Olivier Gauthier, Jean-Michel Bouler, Bruno Bujoli
Two commercial formulations of apatitic calcium phosphate cements (CPCs), Graftys® Quickset (QS) and Graftys® HBS (HBS), similar in composition but with different initial setting time (7 and 15min, respectively), were combined to ovine whole blood. Surprisingly, although a very cohesive paste was obtained after a few minutes, the setting time of the HBS/blood composite dramatically delayed when compared to its QS analogue and the two blood-free references. Using solid state NMR, scanning electron microscopy and high frequency impedance measurements, it was shown that, in the particular case of the HBS/blood composite, formation of a reticulated and porous organic network occurred in the intergranular space, prior to the precipitation of apatite crystals driven by the cement setting process...
August 30, 2017: Acta Biomaterialia
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