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https://www.readbyqxmd.com/read/28100315/flavobacterium-arcticum-sp-nov-isolated-from-arctic-seawater
#1
Dan-Dan Li, Chang Liu, Yan-Qi Zhang, Xiu-Juan Wang, Ning Wang, Ming Peng, Xiao-Yan Song, Hai-Nan Su, Xi-Ying Zhang, Yu-Zhong Zhang, Mei Shi
A Gram-reaction-negative, aerobic, non-flagellated, rod-shaped and yellow-pigmented bacterium, designated strain SM1502T, was isolated from Arctic seawater. The isolate grew at 10-40 oC and with 0-8.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was affiliated with the genus Flavobacterium, with the highest sequence similarity (96.0 %) found with Flavobacterium suzhouense. The major fatty acids of strain SM1502T were iso-C15:0, iso-C17:1ω9c, iso-C15:1 G, C15:0, iso-C17:0 3-OH and unknown ECL 13...
January 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28100313/rectinema-cohabitans-gen-nov-sp-nov-a-rod-shaped-spirochete-isolated-from-an-anaerobic-naphthalene-degrading-enrichment-culture
#2
Janina S Koelschbach, Housna Mouttaki, Carolin Pickl, Hermann J Heipieper, Reinhard Rachel, Paul A Lawson, Rainer U Meckenstock
The anaerobic, non-motile strain HMT was isolated from the naphthalene-degrading, sulfate-reducing enrichment culture N47. Since 20 years, strain HMT has been a stable member of culture N47 although it is neither able to degrade naphthalene nor to reduce sulfate in pure culture. The highest similarity of the 16S rRNA gene sequence of strain HMT (89%) is with a cultivated member of the family Spirochaetaceae, Treponema caldarium sp. strain H1T (DSM 7334T), an obligately anaerobic, thermophilic spirochete isolated from cyanobacterial mat samples collected at a freshwater hot spring in Oregon, USA...
January 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28100309/nioella-sediminis-sp-nov-isolated-from-the-surface-sediment-and-emended-description-of-the-genus-nioella
#3
Yang Liu, Juan Du, Qiliang Lai, Chunming Dong, Zongze Shao
A bacterial strain, designated JS7-11T, was isolated from the surface sediment of the Jiulong River and characterized using a polyphasic taxonomic approach. Cells of strain JS7-11T was Gram-staining-negative, non-motile and short-rod-shaped. Growth was observed at 10-40°C (optimum 25-30°C), at pH 6-8 (optimum 7) and in 1-12% NaCl (optimum 3-4%, w/v). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JS7-11T and type strain Nioella nitratireducens SSW136T formed an independent cluster, sharing 97...
January 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28096349/structure-of-eukaryotic-cmg-helicase-at-a-replication-fork-and-implications-to-replisome-architecture-and-origin-initiation
#4
Roxana Georgescu, Zuanning Yuan, Lin Bai, Ruda de Luna Almeida Santos, Jingchuan Sun, Dan Zhang, Olga Yurieva, Huilin Li, Michael E O'Donnell
The eukaryotic CMG (Cdc45, Mcm2-7, GINS) helicase consists of the Mcm2-7 hexameric ring along with five accessory factors. The Mcm2-7 heterohexamer, like other hexameric helicases, is shaped like a ring with two tiers, an N-tier ring composed of the N-terminal domains, and a C-tier of C-terminal domains; the C-tier contains the motor. In principle, either tier could translocate ahead of the other during movement on DNA. We have used cryo-EM single-particle 3D reconstruction to solve the structure of CMG in complex with a DNA fork...
January 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28091335/high-prevalence-of-hpv-infection-in-the-remote-villages-of-french-guiana-an-epidemiological-study
#5
A Adenis, V Dufit, M Douine, F Corlin, G Ayhan, F Najioullah, V Molinie, P Brousse, G Carles, V Lacoste, R Cesaire, M Nacher
Cervical cancer is the second most frequent cancer in women in French Guiana. Studies have shown that populations living in the remote areas of the interior have early sexual debut and that multiple sexual partnerships are common. The objective of the present study was thus to determine the prevalence of human papillomavirus (HPV) infection in these areas. A study was conducted in women aged 20-65 years with previous sexual activity. Women were included on a voluntary basis after using local media and leaders to inform them of the visit of the team...
January 16, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28077207/proposal-to-reclassify-roseivirga-ehrenbergii-nedashkovskaya-et-al-2008-as-roseivirga-seohaensis-comb-nov-description-of-roseivirga-seohaensis-subsp-aquiponti-subsp-nov-and-emendation-of-the-genus-roseivirga
#6
Chitra Selvaratnam, Suganthi Thevarajoo, Kian Mau Goh, Kok-Gan Chan, Chun Shiong Chong
The genus Roseivirga currently includes five species: Roseivirga ehrenbergii, R. echinicomitans, R. spongicola, R. marina and R. maritima. Marinicola seohaensis SW-152T was renamed as Roseivirgaseohaensis SW-152T and then reclassified again as a later heterotypic synonym of R. ehrenbergii KMM 6017T. In this study, based on average nucleotide identity and digital DNA-DNA hybridization values obtained from in silico methods, together with fatty acid analyses and biochemical tests, we propose to reclassify R. ehrenbergii SW-152 as Roseivirga seohaensis comb...
December 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28076823/guazuma-ulmifolia-bark-synthesized-ag-au-and-ag-au-alloy-nanoparticles-photocatalytic-potential-dna-protein-interactions-anticancer-activity-and-toxicity-against-14-species-of-microbial-pathogens
#7
Viswanathan Karthika, Ayyakannu Arumugam, Kasi Gopinath, Periyannan Kaleeswarran, Marimuthu Govindarajan, Naiyf S Alharbi, Shine Kadaikunnan, Jamal M Khaled, Giovanni Benelli
In the present study, we focused on a quick and green method to fabricate Ag, Au and Ag/Au alloy nanoparticles (NPs) using the bark extract of Guazuma ulmifolia L. Green synthesized metal NPs were characterized using different techniques, including UV-Vis spectroscopy, FT-IR, XRD, AFM and HR-TEM analyses. The production of Ag, Au and Ag/Au alloy NPs was observed monitoring color change from colorless to brown, followed by pink and dark brown, as confirmed by UV-Vis spectroscopy characteristic peaks at 436, 522 and 510nm, respectively...
January 4, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28075137/anisotropic-electroless-deposition-on-dna-origami-templates-to-form-small-diameter-conductive-nanowires
#8
Bibek Uprety, Tyler Westover, Michael Stoddard, Kamron Brinkerhoff, John Jensen, Robert C Davis, Adam T Woolley, John N Harb
An improved method for the metallization of DNA origami is examined in this work. DNA origami, a simple and robust method for creating a wide variety of nanostructured shapes and patterns, provides an enabling template for bottom-up fabrication of next-generation nanodevices. Selective metallization of these DNA templates is needed to make nanoelectronic devices. Here, we demonstrate a metallization process that uses gold nanorod seeds followed by anisotropic plating to provide improved morphology and greater control of the final metallized width of the structure...
January 11, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28074845/local-coexistence-and-genetic-isolation-of-three-pollinator-species-on-the-same-fig-tree-species
#9
T L Sutton, J L DeGabriel, M Riegler, J M Cook
Molecular tools increasingly reveal cryptic lineages and species that were previously unnoticed by traditional taxonomy. The discovery of cryptic species in sympatry prompts the question of how they coexist in the apparent absence of ecological divergence. However, this assumes first that the molecular taxonomy used to identify cryptic lineages delimits species boundaries accurately. This issue is important, because many diversity studies rely heavily or solely on data from mitochondrial DNA sequences for species delimitation, and several factors may lead to poor identification of species boundaries...
January 11, 2017: Heredity
https://www.readbyqxmd.com/read/28073405/rhodanobacter-humi-sp-nov-a-novel-acidtolerant-and-alkalitolerant-gammaproteobacterium-isolated-from-kyonggi-university-forest-soil
#10
Ram Hari Dahal, Jaisoo Kim
A novel acidtolerant and alkalitolerant gammaproteobacterium designated strain RS22T was isolated from Kyonggi University forest soil. Cells were aerobic, Gram-staining-negative, catalase- and oxidase- positive, non-motile, non-spore-forming, rod-shaped, and yellow-pigmented. Flexirubin-type pigments were absent. Strain RS22T assimilates lactic acid, L-proline, and 3-hydroxybenzoic acid; tolerates 4 % of NaCl, ferments glucose, and can grow at pH 11.0. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain RS22T formed a lineage within the class Gammaproteobacteria of the phylum Proteobacteria that was distinct from various species of the genus Rhodanobacter, including Rhodanobacter denitrificans 2APBS1T (97...
January 10, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28070287/mountains-too-high-and-valleys-too-deep-drive-population-structuring-and-demographics-in-a-qinghai-tibetan-plateau-frog-nanorana-pleskei-dicroglossidae
#11
Weiwei Zhou, Jieqiong Jin, Jun Wu, Hongman Chen, Junxiao Yang, Robert W Murphy, Jing Che
Pleistocene glacial-interglacial climatic oscillations greatly shaped the current genetic structure of many species. However, geographic features may influence the impact of climatic cycling. Distinct geographic and environmental characters between northern and southern parts of the eastern Qinghai-Tibetan Plateau (EQTP) facilitate explorations into the impacts of geographic features on species. The northern parts of EQTP contain large areas of marsh, and the environment is rather homogeneous. In contrast, the southern EQTP harbors complex alpine valleys and a much more heterogeneous setting...
January 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28069534/genetic-spatial-structure-of-an-anchialine-cave-annelid-indicates-connectivity-within-but-not-between-islands-of-the-great-bahama-bank
#12
Brett C Gonzalez, Alejandro Martínez, Elizabeth Borda, Thomas M Iliffe, Diego Fontaneto, Katrine Worsaae
Land-locked anchialine blue holes are karstic sinkholes and caves with tidally influenced, vertically stratified water bodies that harbor endemic fauna exhibiting variable troglomorphic features. These habitats represent island-like systems, which can serve to elucidate evolutionary and biogeographic processes at local scales. We investigated whether the 'continuous spelean corridor' hypothesis may elucidate the biogeographical distributions of the stygobitic annelid Pelagomacellicephala iliffei (Polynoidae) collected from the Great Bahama and Caicos Banks of the Bahamas Archipelago...
January 6, 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/28068568/ecogenomic-responses-of-benthic-communities-under-multiple-stressors-along-the-marine-and-adjacent-riverine-areas-of-northern-bohai-sea-china
#13
Yuwei Xie, Seongjin Hong, Seonjin Kim, Xiaowei Zhang, Jianghua Yang, John P Giesy, Tieyu Wang, Yonglong Lu, Hongxia Yu, Jong Seong Khim
Benthic communities in the aquatic ecosystem are influenced by both natural and anthropogenic stressors. To understand the ecogenomic responses of sediment communities to the multiple stressors of polluted environments, the bacteria, protistan and metazoan communities in sediments from marine and adjacent riverine areas of North Bohai Sea were characterized by environmental DNA meta-systematics, and their associations with environmental variables were assessed by multiple statistical approaches. The bacterial communities were dominated by Firmicutes (mean 22...
December 26, 2016: Chemosphere
https://www.readbyqxmd.com/read/28068219/hyphobacterium-vulgare-gen-nov-sp-nov-a-novel-alpha-proteobacterium-isolated-from-seawater-in-the-south-china-sea
#14
Cong Sun, Rui-Jun Wang, Yue Su, Ge-Yi Fu, Zhe Zhao, Xiao-Yun Yu, Chong-Ya Zhang, Can Chen, Shuai-Bo Han, Meng-Meng Huang, Zheng-Bing Lv, Min Wu
A Gram-staining-negative, aerobic, non-spore-forming, non-motile, oval to rod shaped bacteria with prosthecate, designated as WM6T, was isolated from seawater sample collected from the South China Sea at depth of 150 meter and subjected to a polyphasic taxonomic investigation. Cells of strain WM6T were approximately 0.5-0.6 μm in width and 0.8-1.2 μm in length, and colonies of strain WM6T were smooth, circular, convex and whity-yellow. Strain WM6T was found to grow at 10-45 °C (optimum, 30 °C), pH 6.5-9...
January 6, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28068215/tibeticola-sediminis-gen-nov-sp-nov-a-thermophilic-bacterium-isolated-from-hot-spring
#15
Inam Ullah Khan, Firasat Hussain, Ye Tian, Neeli Habib, Wen-Dong Xian, Zhao Jiang, Arshia Amin, Chang-Guo Yuan, En-Min Zhou, Xiao-Yang Zhi, Wen-Jun Li
Two closely related thermophilic bacterial strains, designated YIM 73013T and YIM 73008 were isolated from a sediment sample collected from a hot spring in Tibet, western Tibet province, China. Taxonomic positions of the two isolates were investigated using a polyphasic approach. The novel isolates were Gram-staining negative, aerobic, short rod shaped and motile by means of a polar flagellum. They were oxidase and catalase positive and were able to grow at 30-55 °C (optimum, 37-45 °C), pH 6.0-8.0 (optimum, pH 7...
January 6, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28067319/c-elegans-on-a-chip-for-in-situ-and-in-vivo-ag-nanoparticles-uptake-and-toxicity-assay
#16
Jin Ho Kim, Seung Hwan Lee, Yun Jeong Cha, Sung Jin Hong, Sang Kug Chung, Tai Hyun Park, Shin Sik Choi
Nanomaterials are extensively used in consumer products and medical applications, but little is known about their environmental and biological toxicities. Moreover, the toxicity analysis requires sophisticated instruments and labor-intensive experiments. Here we report a microfluidic chip incorporated with the nematode Caenorhabditis elegans that rapidly displays the changes in body growth and gene expression specifically responsive to the silver nanoparticles (AgNPs). C. elegans were cultured in microfluidic chambers in the presence or absence of AgNPs and were consequently transferred to wedge-shaped channels, which immobilized the animals, allowing the evaluation of parameters such as length, moving distance, and fluorescence from the reporter gene...
January 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28067311/supra-operonic-clusters-of-functionally-related-genes-socs-are-a-source-of-horizontal-gene-co-transfers
#17
Tin Yau Pang, Martin J Lercher
Adaptation of bacteria occurs predominantly via horizontal gene transfer (HGT). While it is widely recognized that horizontal acquisitions frequently encompass multiple genes, it is unclear what the size distribution of successfully transferred DNA segments looks like and what evolutionary forces shape this distribution. Here, we identified 1790 gene family pairs that were consistently co-gained on the same branches across a phylogeny of 53 E. coli strains. We estimated a lower limit of their genomic distances at the time they were transferred to their host genomes; this distribution shows a sharp upper bound at 30 kb...
January 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28057460/a-characterization-of-structural-proteins-expressed-by-bombyx-mori-bidensovirus
#18
Peng Lü, Yali Xing, Zhaoyang Hu, Yanhua Yang, Ye Pan, Kangmin Chen, Feifei Zhu, Yajing Zhou, Keping Chen, Qin Yao
Bombyx mori bidensiovirus (BmBDV) is a species of Bidensovirus that has been was placed into a new genus within the new family Bidnaviridae by the International Committee on Taxonomy of Viruses. BmBDV causes fatal flacherie disease in silkworms, which causes large losses to the sericulture industry. BmBDV contains two sets of complementary linear single-stranded DNAs of approximately 6.5 kb (viral DNA 1, VD1) and 6.0 kb (viral DNA 2, VD2). VD1 and VD2 are encapsidated in separate icosahedral non-enveloped capsids, which are similar in size and shape...
January 2, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28057375/description-of-a-new-anaerobic-thermophilic-bacterium-thermoanaerobacterium-butyriciformans-sp-nov
#19
G López, S J Cañas-Duarte, A M Pinzón-Velasco, N E Vega-Vela, M Rodríguez, S Restrepo, S Baena
Strain USBA-019(T), an anaerobic and thermophilic strain, was identified as a new member of the genus Thermoanaerobacterium. USBA-019(T) cells are gram-positive, strictly anaerobic, thermophilic, chemoorganotrophic, moderately acidophilic, non-motile, endospore-forming, slightly curved, and rod-shaped. Cells measure 0.4×3.0-7.0μm. Optimal growth occurs at 50-55°C (35-65°C). Optimum pH is 5.0-5.5 (4.0-8.5). Thiosulfate, elemental sulfur and nitrate were utilized as electron acceptors. Fermentation of glucose, lactose, cellobiose, galactose, arabinose, xylose, starch and xylan primarily produced acetate and butyrate...
December 16, 2016: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28056225/xenorhabdus-thuongxuanensis-sp-nov-and-xenorhabdus-eapokensis-sp-nov-isolated-from-steinernema-species
#20
Peter Kämpfer, Nicholas J Tobias, Long Phan Ke, Helge B Bode, Stefanie P Glaeser
Two slightly yellowish pigmented, oxidase-negative, rod-shaped and Gram-negative bacterial strains (30TX1T and DL20T), isolated from Steinernema sangi and Steinernema eapokense, respectively, during soil sampling in Vietnam were studied in a polyphasic taxonomic approach. Strain 30TX1T showed highest 16S rRNA gene sequence similarity to Xenorhabdus ehlersii (98.9%) and strain DL20T to Xenorhabdus ishibashii (98.7%). Sequence similarities to all other Xenorhabdus species were lower (< 98.4%). The two strains shared 98% 16S rRNA gene sequence similarity...
January 4, 2017: International Journal of Systematic and Evolutionary Microbiology
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