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https://www.readbyqxmd.com/read/29792930/functional-genomics-for-the-oleaginous-yeast-yarrowia-lipolytica
#1
Kurt Patterson, James Yu, Jenny Landberg, Ivan Chang, Farbod Shavarebi, Virginia Bilanchone, Suzanne Sandmeyer
Oleaginous yeasts are valuable systems for biosustainable production of hydrocarbon-based chemicals. Yarrowia lipolytica is one of the best characterized of these yeast with respect to genome annotation and flux analysis of metabolic processes. Nonetheless, progress is hampered by a dearth of genome-wide tools enabling functional genomics. In order to remedy this deficiency, we developed a library of Y. lipolytica insertion mutants via transposon mutagenesis. The Hermes DNA transposon was expressed to achieve saturation mutagenesis of the genome...
May 21, 2018: Metabolic Engineering
https://www.readbyqxmd.com/read/29792802/the-anti-biofouling-properties-of-superhydrophobic-surfaces-are-short-lived
#2
Gi Byoung Hwang, Kristopher Page, Adnan Patir, Sean P Nair, Elaine Allan, Ivan P Parkin
Superhydrophobic surfaces are present in nature on the leaves of many plant species. Water rolls on these surfaces and the rolling motion picks up particles including bacteria and viruses. Man-made superhydrophobic surfaces have been made in an effort to reduce biofouling. We show here that the anti-biofouling property of a superhydrophobic surface is due to an entrapped air-bubble layer that reduces contact between the bacteria and the surface. Further, we show that prolonged immersion of superhydrophobic surfaces in water led to loss of the bubble-layer and subsequent bacterial adhesion that unexpectedly, exceeded that of the control materials...
May 24, 2018: ACS Nano
https://www.readbyqxmd.com/read/29792246/servants-of-the-dying-how-the-death-of-ivan-ilyich-informs-hospice-care-today
#3
Aldis H Petriceks
No abstract text is available yet for this article.
May 24, 2018: Palliative & Supportive Care
https://www.readbyqxmd.com/read/29792108/does-maximalist-footwear-lower-impact-loading-during-level-ground-and-downhill-running
#4
Zoe Y S Chan, Ivan P H Au, Fannie O Y Lau, Eric C K Ching, Janet H Zhang, Roy T H Cheung
A new model of running shoes which features an extreme cushioning and an oversized midsole, known as the maximalist (MAX) was launched. This design claims to provide excellent shock absorption, particularly during downhill running. This study sought to assess the effects of MAX on the external impact loading, footstrike pattern, and stride length during level ground and downhill running on an instrumented treadmill. Twenty-seven distance runners completed four 5-minute running trials in the two footwear conditions (MAX and traditional running shoes (TRS)) on a level surface (0%) and downhill (10%-declination)...
May 24, 2018: European Journal of Sport Science
https://www.readbyqxmd.com/read/29792106/psychometric-properties-of-the-photograph-series-of-daily-activities-short-electronic-version-phoda-sev-in-patients-with-chronic-low-back-pain-according-to-cosmin-checklist
#5
Crystian Bitencourt Oliveira, Marcia Rodrigues Franco, Samantha Janaina Demarchi, Rob Johannes E M Smeets, Ivan P J Huijnen, Priscila Kalil Morelhão, Thalysi Mayumi Hisamatsu, Rafael Zambelli Pinto
Study Design A prospective cohort study. Background The Photograph Daily Activities Series - Short Electronic Version (PHODA-SeV) assesses perceived harmfulness of daily activities in patients with low back pain (LBP). Although there is some evidence that the PHODA-SeV is a reliable and valid tool, its psychometric properties have not been fully investigated. Objectives To investigate the test-retest reliability, measurement error, interpretability, construct validity, internal and external responsiveness of the PHODA-SeV in patients with chronic LBP...
May 23, 2018: Journal of Orthopaedic and Sports Physical Therapy
https://www.readbyqxmd.com/read/29791971/predictors-of-treatment-success-after-collagenase-clostridium-histolyticum-injection-for-peyronie-s-disease-development-of-a-nomogram-from-a-multicentre-single-arm-non-placebo-controlled-clinical-study
#6
Andrea Cocci, Giorgio Ivan Russo, Alberto Briganti, Andrea Salonia, Giovanni Cacciamani, Marco Capece, Marco Falcone, Massimiliano Timpano, Gianmartin Cito, Paolo Verze, Bruno Giammusso, Giuseppe Morgia, Vincenzo Mirone, Andrea Minervini, Mauro Gacci, Tommaso Cai, Sergio Serni, Marco Carini, Gianluca Giubilei, Nicola Mondaini
OBJECTIVE: To build-up a nomogram able to predict treatment success after Collagenase clostridium histolyticum (CCH) for Peyronie's disease (PD). MATERIAL AND METHODS: From November 2016 to November 2017, we enrolled 135 patients with PD in a multicentre single-arm prospective study. All patients enrolled had a treatment with CCH. Success of therapy was defined as a penile curvature decrease of at least 20 degrees from baseline curvature. Treatment satisfaction was assessed using a scale from 1 to 10 and high satisfaction was arbitral defined as a score ≥8...
May 23, 2018: BJU International
https://www.readbyqxmd.com/read/29791888/boosted-nutritional-quality-of-food-by-co-2-enrichment-fails-to-offset-energy-demand-of-herbivores-under-ocean-warming-causing-energy-depletion-and-mortality
#7
Jonathan Y S Leung, Ivan Nagelkerken, Bayden D Russell, Camilo M Ferreira, Sean D Connell
The CO2 -boosted trophic transfer from primary producers to herbivores has been increasingly discovered at natural CO2 vents and in laboratory experiments. Despite the emerging knowledge of this boosting effect, we do not know the extent to which it may be enhanced or dampened by ocean warming. We investigated whether ocean acidification and warming enhance the nutritional quality (C:N ratio) and energy content of turf algae, which is speculated to drive higher feeding rate, greater energy budget and eventually faster growth of herbivores...
May 20, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29791853/p27-kip1-modulates-axonal-transport-by-regulating-%C3%AE-tubulin-acetyltransferase-1-stability
#8
Giovanni Morelli, Aviel Even, Ivan Gladwyn-Ng, Romain Le Bail, Michal Shilian, Juliette D Godin, Elise Peyre, Bassem A Hassan, Arnaud Besson, Jean-Michel Rigo, Miguel Weil, Bert Brône, Laurent Nguyen
The protein p27Kip1 plays roles that extend beyond cell-cycle regulation during cerebral cortex development, such as the regulation of neuronal migration and neurite branching via signaling pathways that converge on the actin and microtubule cytoskeletons. Microtubule-dependent transport is essential for the maturation of neurons and the establishment of neuronal connectivity though synapse formation and maintenance. Here, we show that p27Kip1 controls the transport of vesicles and organelles along the axon of mice cortical projection neurons in vitro...
May 22, 2018: Cell Reports
https://www.readbyqxmd.com/read/29791845/equilibrative-nucleoside-transporter-3-regulates-t-cell-homeostasis-by-coordinating-lysosomal-function-with-nucleoside-availability
#9
Chin-Wen Wei, Chia-Ying Lee, Ding-Jin Lee, Chang-Feng Chu, Ju-Chu Wang, Tien-Chiao Wang, Wann-Neng Jane, Zee-Fen Chang, Chuen-Miin Leu, Ivan L Dzhagalov, Chia-Lin Hsu
T cells are a versatile immune cell population responding to challenges by differentiation and proliferation followed by contraction and memory formation. Dynamic metabolic reprogramming is essential for T cells to meet the biosynthetic needs and the reutilization of biomolecules, processes that require active participation of metabolite transporters. Here, we show that equilibrative nucleoside transporter 3 (ENT3) is highly expressed in peripheral T cells and has a key role in maintaining T cell homeostasis by supporting the proliferation and survival of T cells...
May 22, 2018: Cell Reports
https://www.readbyqxmd.com/read/29791674/disseminating-health-evidence-summaries-to-increase-evidence-use-in-health-care
#10
Maria Cristiane Barbosa Galvao, Fabio Carmona, Roland Grand, Pierre Pluye, Ivan Luiz Marques Ricarte
OBJECTIVE: To verify whether an intervention based on disseminating health evidence summaries by e-mail to health professionals increases access to health evidence databases, and whether health professionals intend to apply the evidence received by e-mail in their clinical practice. METHODS: This quantitative study started with a survey to collect demographic data and patterns of access to health evidence databases. It was followed by a longitudinal intervention, over 48 weeks, that disseminated 143 health evidence summaries to 339 health professionals with higher education degree who work in the Brazilian Unified Health System...
2018: Revista de Saúde Pública
https://www.readbyqxmd.com/read/29791416/oncological-outcomes-in-extended-time-intervals-betweenpreoperative-chemoradiotherapy-with-capecitabine-and-surgery-inoperable-rectal-adenocarcinoma
#11
Miguel A Muñoz, Manuel G Palacios, Jenny Malca, Raúl Mantilla, Paola C Montenegro, Ivan Chavez
OBJECTIVE: To assess whether extended time intervals (8-12, 13-20 and >20 weeks) between the end of neoadjuvant chemoradiotherapy and surgery affect overall survival, disease-free survival. MATERIALS AND METHODS: Retrospective study in 120 patients with rectal adenocarcinoma without evidence of metastasis (T1-4/N0-2/M0) at the time of diagnosis that underwent surgery with curative intent after neoadjuvant chemoradiotherapy with capecitabine and obtained R0 or R1 resection between January 2010 to December 2014 at the National Cancer Institute of Peru...
January 2018: Revista de Gastroenterología del Perú: órgano Oficial de la Sociedad de Gastroenterología del Perú
https://www.readbyqxmd.com/read/29791300/which-chart-elements-accurately-identify-emergency-department-visits-for-suicidal-ideation-or-behavior
#12
Sarah A Arias, Edwin D Boudreaux, Elizabeth Chen, Ivan Miller, Carlos A Camargo, Richard N Jones, Lisa Uebelacker
OBJECTIVE: In an emergency department (ED) sample, we investigated the concordance between identification of suicide-related visits through standardized comprehensive chart review versus a subset of three specific chart elements: ICD-9-CM codes, free-text presenting complaints, and free-text physician discharge diagnoses. METHODS: Review of medical records for adults (≥18 years) at eight EDs across the United States. RESULTS: A total of 3,776 charts were reviewed...
May 23, 2018: Archives of Suicide Research: Official Journal of the International Academy for Suicide Research
https://www.readbyqxmd.com/read/29791176/-a-consensual-therapeutic-recommendation-for-type-2-diabetes-mellitus-by-the-slovak-diabetes-society-2018
#13
Emil Martinka, Vladimír Uličiansky, Marián Mokáň, Ivan Tkáč, Peter Galajda, Silvia Dókušová, Zbynek Schroner
Type 2 diabetes mellitus is a heterogeneous medical condition involving multiple pathophysiological mechanisms. Its successful treatment requires an individualized approach and frequently combined therapy with utilizing its effect on multiple levels. Current possibilities enable the employment of such procedures to an incomparably greater extent than before. The effects of different classes of oral antidiabetic drugs on the reduction of glycemia and HbA1c is mutually comparable. However differences are observed in the proportions of patients who met the required criteria, regarding the increase in weight, incidence of hypoglycemia as well as the effect on cardiovascular, renal or oncologic morbidity and mortality, and severity of specific adverse effects, potential risks and contraindications...
2018: Vnitr̆ní Lékar̆ství
https://www.readbyqxmd.com/read/29791157/a-novel-hybrid-correlation-energy-hyce-approach-based-on-separate-evaluations-of-internal-and-external-components
#14
Joseph Ivanic, Michael W Schmidt
A novel Hybrid Correlation Energy (HyCE) approach is proposed that determines the total correlation energy via distinct computation of its internal and external components. This approach evolved from two related studies. First, rigorous assessment of the accuracies and size extensivities of a number of electron correlation methods, that include perturbation theory (PT2), coupled-cluster (CC), configuration interaction (CI) and coupled electron pair approximation (CEPA), shows that the CEPA(0) variant of the latter and triples-corrected CC methods consistently perform very similarly...
May 23, 2018: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/29791056/dactylomonas-gen-nov-a-novel-lineage-of-heterolobosean-flagellates-with-unique-ultrastructure-closely-related-to-the-amoeba-selenaion-koniopes-park-de-jonckheere-simpson-2012
#15
Pavla Hanousková, Petr Táborský, Ivan Čepička
We report the discovery of a new genus of heterolobosean flagellates, Dactylomonas gen. nov., with two species, D. venusta sp. nov. and D. crassa sp. nov. Phylogenetic analysis of the SSU rRNA gene showed that Dactylomonas is closely related to the amoeba Selenaion, the deepest-branching lineage of Tetramitia. Dactylomonads possess two flagella, and ultrastructural studies revealed an unexpected organization of the flagellar apparatus, which resembled Pharyngomonada (the second lineage of Heterolobosea) instead of Tetramitia: basal bodies were orthogonal to each other and a putative root R1 was present in the mastigont...
May 23, 2018: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/29790994/european-public-health-news
#16
Dineke Zeegers Paget, Natasha Azzopardi Muscat, Dineke Zeegers Paget, Zsuzsanna Jakab, Vytenis Andriukaitis, Ivan Eržen
No abstract text is available yet for this article.
June 1, 2018: European Journal of Public Health
https://www.readbyqxmd.com/read/29789838/elastomeric-microvalve-geometry-affects-haemocompatibility
#17
Crispin Szydzik, Rose J Brazilek, Khashayar Khoshmanesh, Farzan Akbaridoust, Markus Knoerzer, Peter Thurgood, Ineke Muir, Ivan Marusic, Harshal Nandurkar, Arnan Mitchell, Warwick S Nesbitt
This paper reports on the parameters that determine the haemocompatibility of elastomeric microvalves for blood handling in microfluidic systems. Using a comprehensive investigation of blood function, we describe a hierarchy of haemocompatibility as a function of microvalve geometry and identify a "normally-closed" v-gate pneumatic microvalve design that minimally affects blood plasma fibrinogen and von Willebrand factor composition, minimises effects on erythrocyte structure and function, and limits effects on platelet activation and aggregation, while facilitating rapid switching control for blood sample delivery...
May 23, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29789531/mitochondrial-oxphos-influences-immune-cell-fate-lessons-from-hematopoietic-aif-deficient-and-ndufs4-deficient-mouse-models
#18
Audrey Bertaux, Lauriane Cabon, Marie-Noëlle Brunelle-Navas, Sandrine Bouchet, Ivan Nemazanyy, Santos A Susin
No abstract text is available yet for this article.
May 22, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29789486/prevalence-and-risk-factors-for-hepatitis-b-virus-infection-in-roma-and-non-roma-people-in-slovakia
#19
Sylvia Drazilova, Martin Janicko, Pavol Kristian, Ivan Schreter, Monika Halanova, Ingrid Urbancikova, Andrea Madarasova-Geckova, Maria Marekova, Daniel Pella, Peter Jarcuska
Prevalence of Hepatitis B is relatively low in developed European countries. However specific subpopulations may exist within each country with markedly different Hepatitis B burden. Roma minority is very numerous in Slovakia and their lifestyle is completely different to non-Roma population. The aim of this study is to map Hepatitis B prevalence in Roma and compare it to non-Roma population and to explore potential socio-economic and health related risk factors. Cross-sectional epidemiology study was performed in Slovakia that included randomly sampled Roma population and geographically corresponding random sampled non-Roma population...
May 22, 2018: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29789435/molecular-mechanisms-preventing-senescence-in-response-to-prolonged-darkness-in-a-desiccation-tolerant-plant
#20
Meriem Durgud, Saurabh Gupta, Ivan Ivanov, Amin Omidbakhshfard, Maria Benina, Saleh Alseekh, Nikola Staykov, Mareike Hauenstein, Paul Dijkwel, Stefan Hörtensteiner, Valentina Toneva, Yariv Brotman, Alisdair R Fernie, Bernd Mueller-Roeber, Tsanko Gechev
The desiccation-tolerant plant Haberlea rhodopensis can withstand months of darkness without any visible senescence. Here, we investigated the molecular mechanisms of this adaptation to prolonged (30 days) darkness and subsequent return to light. H. rhodopensis plants remained green and viable throughout the dark treatment. Transcriptomic analysis revealed that darkness regulated several transcription factor (TF) genes. Stress- and autophagy-related TFs such as ERF8, HSFA2b, RD26, TGA1, and WRKY33 were upregulated, while chloroplast- and flowering-related TFs such as ATH1, COL2, COL4, RL1, and PTAC7 were repressed...
May 22, 2018: Plant Physiology
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