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https://www.readbyqxmd.com/read/28507242/an-rpohi-dependent-response-promotes-outgrowth-after-extended-stationary-phase-in-the-alphaproteobacterium-rhodobacter-sphaeroides
#1
B Remes, T Rische-Grahl, K M H Müller, K U Förstner, Y Sung-Huan, L Weber, A Jäger, V Peuser, G Klug
Under unfavorable growth conditions, bacteria enter stationary phase and can maintain cell viability over prolonged periods with no increase in cell number. To get insights into the regulatory mechanisms that allow bacteria to resume growth when conditions become favorable again (outgrowth) we performed global transcriptome analyses at different stages of growth for the alphaproteobacterium Rhodobacter sphaeroides The majority of genes was not differentially expressed across growth phases. After short stationary phase (about 20 h after growth starts to slow down), only 7 % of the genes showed altered expression (fold change of > 1...
May 15, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28497282/highly-complex-substrates-lead-to-dynamic-bacterial-community-for-polyhydroxyalkanoates-production
#2
Diogo Queirós, Alexandre Fonseca, Simona Rossetti, Luísa S Serafim, Paulo C Lemos
Mixed microbial cultures (MMC) and waste/surplus substrates, as hardwood spent sulfite liquor, are being used to decrease polyhydroxyalkanoates' (PHA) production costs. The process involves two or three steps, being the selection step a crucial one. For the industrial implementation of this strategy, reactor stability in terms of both performance and microbial community presence has to be considered. A long-term operation of a sequencing batch reactor under feast/famine conditions was performed along with microbial community identification/quantification using FISH and DGGE...
May 11, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28471051/a-presumed-antagonistic-lps-identifies-distinct-functional-organization-of-tlr4-in-mouse-microglia
#3
Christin Döring, Tommy Regen, Ulla Gertig, Denise van Rossum, Anne Winkler, Nasrin Saiepour, Wolfgang Brück, Uwe-Karsten Hanisch, Hana Janova
Microglia as principle innate immune cells of the central nervous system (CNS) are the first line of defense against invading pathogens. They are capable of sensing infections through diverse receptors, such as Toll-like receptor 4 (TLR4). This receptor is best known for its ability to recognize bacterial lipopolysaccharide (LPS), a causative agent of gram-negative sepsis and septic shock. A putative, naturally occurring antagonist of TLR4 derives from the photosynthetic bacterium Rhodobacter sphaeroides. However, the antagonistic potential of R...
May 4, 2017: Glia
https://www.readbyqxmd.com/read/28469140/mitochondria-targeted-antioxidants-as-highly-effective-antibiotics
#4
Pavel A Nazarov, Ilya A Osterman, Artem V Tokarchuk, Marina V Karakozova, Galina A Korshunova, Konstantin G Lyamzaev, Maxim V Skulachev, Elena A Kotova, Vladimir P Skulachev, Yuri N Antonenko
Mitochondria-targeted antioxidants are known to alleviate mitochondrial oxidative damage that is associated with a variety of diseases. Here, we showed that SkQ1, a decyltriphenyl phosphonium cation conjugated to a quinone moiety, exhibited strong antibacterial activity towards Gram-positive Bacillus subtilis, Mycobacterium sp. and Staphylococcus aureus and Gram-negative Photobacterium phosphoreum and Rhodobacter sphaeroides in submicromolar and micromolar concentrations. SkQ1 exhibited less antibiotic activity towards Escherichia coli due to the presence of the highly effective multidrug resistance pump AcrAB-TolC...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28465227/bovine-lactoferrin-reduces-extra-territorial-facial-allodynia-hyperalgesia-following-a-trigeminal-nerve-injury-in-the-rat
#5
Kayo Horie, Mineo Watanabe, Chea Chanbora, Tetsuya Awada, Ryo Kunimatsu, Takashi Uchida, Takashi Takata, Kotaro Tanimoto
There is an urgent clinical need for an effective therapeutic agent to treat neuropathic pain. This study explored whether intrathecal administration of bovine lactoferrin (bLF), in combination with signal transduction pathway inhibition or an inflammatory cytokine production, results in reduced allodynia/hyperalgesia in the whisker pad area following mental nerve transection (MNT) in rats. Rats were intrathecally infused with bLF, lipopolysaccharide from Rhodobacter sphaeroides (LPS-RS), an antagonist of Toll-like receptor 4 (TLR4), or interleukin (IL)-18 binding protein (BP)...
April 29, 2017: Brain Research
https://www.readbyqxmd.com/read/28461447/bacterial-pero-permeases-transport-sulfate-and-related-oxyanions
#6
Marie-Christine Hoffmann, Yvonne Pfänder, Marc Tintel, Bernd Masepohl
Rhodobacter capsulatus synthesizes the high-affinity ABC transporters CysTWA and ModABC to specifically import the chemically related oxyanions sulfate and molybdate, respectively. In addition, R. capsulatus has the low-affinity permease PerO acting as a general oxyanion transporter, whose elimination increases tolerance to molybdate and tungstate. Although widespread in bacteria, the function of PerO-like permeases has not been examined in any other species to date. Here, we present evidence that PerO permeases from the Alphaproteobacteria Agrobacterium tumefaciens, Dinoroseobacter shibae, Rhodobacter sphaeroides, and Sinorhizobium meliloti, and the Gammaproteobacterium Pseudomonas stutzeri functionally substitute for R...
May 1, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28438349/hydrogen-bonds-in-the-vicinity-of-the-special-pair-of-the-bacterial-reaction-center-probed-by-hydrostatic-high-pressure-absorption-spectroscopy
#7
Liina Kangur, Michael R Jones, Arvi Freiberg
Using the native bacteriochlorophyll a pigment cofactors as local probes, we investigated the response to external hydrostatic high pressure of reaction center membrane protein complexes from the photosynthetic bacterium Rhodobacter sphaeroides. Wild-type and engineered complexes were used with a varied number (0, 1 or 2) of hydrogen bonds that bind the reaction center primary donor bacteriochlorophyll cofactors to the surrounding protein scaffold. A pressure-induced breakage of hydrogen bonds was established for both detergent-purified and membrane-embedded reaction centers, but at rather different pressures: between 0...
April 7, 2017: Biophysical Chemistry
https://www.readbyqxmd.com/read/28433189/synergic-regulation-of-redox-potential-and-oxygen-uptake-to-enhance-production-of-coenzyme-q10-in-rhodobacter-sphaeroides
#8
Yongqiang Zhu, Lidan Ye, Zhaofeng Chen, Weijiang Hu, Yanghui Shi, Jianbo Chen, Chenfei Wang, Yong Li, Weifeng Li, Hongwei Yu
The physiological role of Coenzyme Q10 (CoQ10) as an electron carrier suggests its association with redox potential. Overexpression of glyceraldehyde-3-phosphate dehydrogenase type I (gapA-1) in Rhodobacter sphaeroides elevated the NADH/NAD(+) ratio and meanwhile enhanced the CoQ10 content by 58%, but at the sacrifice of biomass. On the other hand, Vitreoscilla hemoglobin was heterologously expressed to enhance the oxygen uptake ability of the cells, leading to 127% improvement of biomass. Subsequent coexpression of gapA-1 and vgb resulted in a CoQ10 titer of 83...
June 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28427254/supplementing-the-culture-of-rhodobacter-sphaeroides-in-the-diet-of-holstein-lactating-cow-may-naturally-produce-coenzyme-q10-enriched-milk
#9
Gui-Seck Bae, Ahreum Choi, Joon Mo Yeo, Jong Nam Kim, Jaeyong Song, Eun Joong Kim, Moon Baek Chang
Objective: To examine the effects of Rhaodobacter sphaeroides supplementation as a direct-fed microbial (DFM) on rumen fermentation in dairy cows and on CoQ10 transition into milk, an in vitro rumen simulation batch culture and an in vivo dairy cow experiments were conducted. Methods: The characteristics of in vitro ruminal fermentation were investigated using rumen fluids from six cannulated Holstein dairy cows at 2 h post-afternoon feeding. A control treatment was included in the experiments based on a typified total mixed ration (TMR) for lactating dairy cows, which was identical to the one used in the in vivo study, plus R...
April 21, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28417144/the-effect-of-reconstruction-works-on-planktonic-bacterial-diversity-of-a-unique-thermal-lake-revealed-by-cultivation-molecular-cloning-and-next-generation-sequencing
#10
Gergely Krett, Attila Szabó, Tamás Felföldi, Károly Márialigeti, Andrea K Borsodi
The aim of this study was to gain detailed information about the diversity of planktonic bacterial communities of a worldwide special peat bedded natural thermal spa lake, and to reveal the effect of a lake wall reconstruction work. To compare the efficiency of different methods used for analyzing bacterial diversity, cultivation, molecular cloning and pyrosequencing were applied simultaneously. Despite the almost unchanged physical-chemical parameters and cell count values of lake water, remarkable differences were observed in the planktonic bacterial community structures during and after the reconstruction by all applied microbiological approaches...
April 17, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28411470/incorporation-of-spheroidene-and-spheroidenone-into-light-harvesting-complexes-from-purple-sulfur-bacteria
#11
Aleksandr Ashikhmin, Zoya Makhneva, Maksim Bolshakov, Andrey Moskalenko
Spheroidene and spheroidenone from the non-sulfur bacterium Rhodobacter (Rba.) sphaeroides were incorporated into diphenylamine (DPA) LH1-RC and LH2 complexes from sulfur bacteria Allochromatium (Alc.) minutissimum and Ectothiorhodospira (Ect.) haloalkaliphila in which carotenoid (Car) biosynthesis was inhibited by ~95%. A series of biochemical characteristics of the modified LH2 complexes was studied (electrophoretic mobility, absorption and CD spectra, Car composition, Car-to-BChl energy transfer and thermal stability)...
March 30, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28409433/reusing-colored-industrial-wastewaters-in-a-photofermentation-for-enhancing-biohydrogen-production-by-using-ultrasound-stimulated-rhodobacter-sphaeroides
#12
Pretty Mori Budiman, Ta Yeong Wu, Ramakrishnan Nagasundara Ramanan, Jamaliah Md Jahim
One-time ultrasonication pre-treatment of Rhodobacter sphaeroides was evaluated for improving biohydrogen production via photofermentation. Batch experiments were performed by varying ultrasonication amplitude (15, 30, and 45%) and duration (5, 10, and 15 min) using combined effluents from palm oil as well as pulp and paper mill as a single substrate. Experimental data showed that ultrasonication at amplitude 30% for 10 min (256.33 J/mL) achieved the highest biohydrogen yield of 9.982 mL H2/mLmedium with 5...
April 13, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28402874/a-molecular-view-of-the-dynamics-of-dsdna-packing-inside-viral-capsids-in-the-presence-of-ions
#13
Andrés Córdoba, Daniel M Hinckley, Joshua Lequieu, Juan J de Pablo
Genome packing in viruses and prokaryotes relies on positively charged ions to reduce electrostatic repulsions, and induce attractions that can facilitate DNA condensation. Here we present molecular dynamics simulations spanning several microseconds of dsDNA packing inside nanometer-sized viral capsids. We use a detailed molecular model of DNA that accounts for molecular structure, basepairing, and explicit counterions. The size and shape of the capsids studied here are based on the 30-nanometer-diameter gene transfer agents of bacterium Rhodobacter capsulatus that transfer random 4...
April 11, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28389962/purification-of-fla2-flagella-of-rhodobacter-sphaeroides
#14
Javier de la Mora, Laura Camarena, Georges Dreyfus
The photosynthetic bacterium R. sphaeroides expresses two flagellar systems that are encoded by two complete gene clusters that have distinct phylogenetic origins. The isolation and purification of the Filament-Hook Basal Body (F-HBB) or the Hook Basal Body (HBB) structure is a troublesome task given the complexity of this nano-machine that is composed of multiple loosely bound substructures that can be lost during the isolation and purification procedure. A successful procedure requires adjustments to the standard method established for Salmonella...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28386667/dynamic-modeling-of-temperature-change-in-outdoor-operated-tubular-photobioreactors
#15
Dominic Deo Androga, Basar Uyar, Harun Koku, Inci Eroglu
In this study, a one-dimensional transient model was developed to analyze the temperature variation of tubular photobioreactors operated outdoors and the validity of the model was tested by comparing the predictions of the model with the experimental data. The model included the effects of convection and radiative heat exchange on the reactor temperature throughout the day. The temperatures in the reactors increased with increasing solar radiation and air temperatures, and the predicted reactor temperatures corresponded well to the measured experimental values...
April 6, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28382525/5-aminolevulinic-acid-production-from-inexpensive-glucose-by-engineering-the-c4-pathway-in-escherichia-coli
#16
Wenwen Ding, Huanjiao Weng, Guocheng Du, Jian Chen, Zhen Kang
5-Aminolevulinic acid (ALA), the first committed intermediate for natural biosynthesis of tetrapyrrole compounds, has recently drawn intensive attention due to its broad potential applications. In this study, we describe the construction of recombinant Escherichia coli strains for ALA production from glucose via the C4 pathway. The hemA gene from Rhodobacter capsulatus was optimally overexpressed using a ribosome binding site engineering strategy, which enhanced ALA production substantially from 20 to 689 mg/L...
April 5, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28374036/dependence-of-the-hydration-status-of-bacterial-light-harvesting-complex-2-on-polyol-cosolvents
#17
Ying Shi, Jie Yu, Long-Jiang Yu, Peng Wang, Li-Min Fu, Jian-Ping Zhang, Zheng-Yu Wang-Otomo
Low molecular weight (MW) polyols are organic osmolytes influencing protein structure and activity. We have intended to investigate the effects of low MW polyols on the optical and the excited-state properties of the light-harvesting complex 2 (LH2) isolated from the photosynthetic bacterium Thermochromatium (Tch.) tepidum, a thermophile growing at ∼50 °C. Steady state spectroscopy demonstrated that, on increasing glycerol or sorbitol fractions up to 60% (polyol/water, v/v), the visible absorption of carotenoids (Crts) remained unchanged, while the near infrared Qy absorption of bacteriochlorophyll a (BChl) at 800 nm (B800) and 850 nm (B850) varied slightly...
May 17, 2017: Photochemical & Photobiological Sciences
https://www.readbyqxmd.com/read/28348828/the-rega-regulon-exhibits-variability-in-response-to-altered-growth-conditions-and-differs-markedly-between-rhodobacter-species
#18
Heidi S Schindel, Carl E Bauer
The RegB/RegA two-component system from Rhodobacter capsulatus regulates global changes in gene expression in response to alterations in oxygen levels. Studies have shown that RegB/RegA controls many energy-generating and energy-utilizing systems such as photosynthesis, nitrogen fixation, carbon fixation, hydrogen utilization, respiration, electron transport and denitrification. In this report, we utilized RNA-seq and ChIP-seq to analyse the breadth of genes indirectly and directly regulated by RegA. A comparison of mRNA transcript levels in wild type cells relative to a RegA deletion strain shows that there are 257 differentially expressed genes under photosynthetic defined minimal growth medium conditions and 591 differentially expressed genes when grown photosynthetically in a complex rich medium...
October 2016: Microbial Genomics
https://www.readbyqxmd.com/read/28325764/the-vitamin-b12-dependent-photoreceptor-aerr-relieves-photosystem-gene-repression-by-extending-the-interaction-of-crtj-with-photosystem-promoters
#19
Mingxu Fang, Carl E Bauer
Purple nonsulfur bacteria adapt their physiology to a wide variety of environmental conditions often through the control of transcription. One of the main transcription factors involved in controlling expression of the Rhodobacter capsulatus photosystem is CrtJ, which functions as an aerobic repressor of photosystem genes. Recently, we reported that a vitamin B12 binding antirepressor of CrtJ called AerR is required for anaerobic expression of the photosystem. However, the mechanism whereby AerR regulates CrtJ activity is unclear...
March 21, 2017: MBio
https://www.readbyqxmd.com/read/28301162/determination-of-cell-doubling-times-from-the-return-on-investment-time-of-photosynthetic-vesicles-based-on-atomic-detail-structural-models
#20
Andrew Hitchcock, C Neil Hunter, Melih Sener
Cell doubling times of the purple bacterium Rhodobacter sphaeroides during photosynthetic growth are determined experimentally and computationally as a function of illumination. For this purpose, energy conversion processes in an intracytoplasmic membrane vesicle, the chromatophore, are described based on an atomic detail structural model. The cell doubling time and its illumination dependence are computed in terms of the return-on-investment (ROI) time of the chromatophore, determined computationally from the ATP production rate, and the mass ratio of chromatophores in the cell, determined experimentally from whole cell absorbance spectra...
April 20, 2017: Journal of Physical Chemistry. B
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