photofermentation

Increased production of renewable energy sources is becoming increasingly needed. Amidst other strategies, one promising technology that could help achieve this goal is biological hydrogen production. This technology uses micro-organisms to convert organic matter into hydrogen gas, a clean and versatile fuel that can be used in a wide range of applications. While biohydrogen production is in its early stages, several challenges must be addressed for biological hydrogen production to become a viable commercial solution...

Hydrogen production from biological processes has been hailed as a promising strategy for generating sustainable energy. Fermentative hydrogen production processes such as dark and photofermentation are considered more sustainable and economical than other biological methods such as biophotolysis. However, these methods have constraints such as low hydrogen yield and conversion efficiency, so practical implementations still need to be made. The present review provides an assessment and feasibility of producing biohydrogen through dark and photofermentation techniques utilizing various lignocellulosic biomass wastes as substrates...

As the energy crisis has been getting increasingly serious in recent years, the research on biohydrogen (bioH2 ) production as a renewable energy source has been a high-demanding subject. It aims to reduce the pressures set by CO2 emissions and the depletion of fossil fuel supplies. Though bioH2 science is still at the beginning, there have been a lot of reported works on lab and pilot-scale systems with promising futures. This paper presents various potential methods of bioH2 production using biomass resources and comparatively assesses them for environmental impacts with a special emphasis on biological processes...

Photofermentative hydrogen production has gained increasing attention as a source of green energy. To make such photofermentation processes economically competitive, operating costs need to be reduced, possibly through outdoor operation. Because photofermentation processes are light dependent, the emission spectrum and intensity of light both have a significant influence on the hydrogen production and merit investigation. This study investigates the effect of light sources on the hydrogen production and growth of Rhodopseudomonas palustris, comparing the organism's productivity under longer-wavelength light and light mimicking sunlight...

Hydrogen production via microbial photofermentation shows great promise as a method for sustainable hydrogen production; however, operating costs associated with photofermentative hydrogen production need to be reduced. Costs can be reduced using a passive circulation system like the thermosiphon photobioreactor, and by operating it under natural sunlight. In this study, an automated system was implemented to investigate the effect of diurnal light cycles on the hydrogen productivity and growth of Rhodopseudomonas palustris and on the operation of a thermosiphon photobioreactor, under controlled conditions...

This work aimed to obtain polyhydroxyalkanoates (PHA) and 5-aminolevulinic acid (5-ALA) from medium-chain carboxylic acids (MCCA) using a mixed culture enriched in Rhodopseudomnas palustris. MCCA, obtained from residual wine lees, were tested in batch photofermentation experiments. First, the influence of individual MCCA (hexanoic, heptanoic, and octanoic acids) was evaluated; then, the MCCA coming directly from a fermentation reactor (LC-effluent) or after acids extraction (HC-effluent) were studied. Nutrient supplementation, bicarbonate, and acetic acid addition were also tested...

One of the main factors affecting hydrogen production and growth of photofermentative microorganisms is light; low light penetration and utilization are significant bottlenecks in photofermentative hydrogen production systems. In this study, light distribution in a thermosiphon photobioreactor operated with Rhodopseudomonas palustris was investigated. Radiation fields were modelled and simulated using computational fluid dynamics (ANSYS® Fluent, 2019 R2) and a reflector system was evaluated for the enhancement of light distribution in a thermosiphon photobioreactor...

Photosynthetic biohydrogen has the advantages of extensive raw materials, clean and renewable, etc. But, its low substrate utilization rate limit its commercial application. It is reported that the use of additives in the process of biohydrogen by photofermentation is beneficial to increase biohydrogen. However, in practical application, the mechanism of additives in hydrogen production is not understood. This paper, the promotion effect of some additives on biohydrogen by photofermentation was reviewed. Whatever, the existing problems and development trends of various additives are also discussed...

A thermosiphon photobioreactor (TPBR) can potentially be used for biohydrogen production, circumventing the requirement for external mixing energy inputs. In this study, a TPBR is evaluated for photofermentative hydrogen production by Rhodopseudomonas palustris ( R. palustris ). Experiments were conducted in a TPBR, and response surface methodology (RSM), varying biomass concentration, and light intensity and temperature were employed to determine the operating conditions for the enhancement of both hydrogen production as well as biomass suspension...

The current work provides insights for improving the hydrogen output while degrading emerging contaminants using Rhodopseudomonas palustris. The changes in the growth rate of a microorganism due to different substrate inputs affects the hydrogen production due to metabolic route changes. The different ratios of glutamate and glycerol as nitrogen and carbon sources along with the presence of ethinylestradiol (EE2) in the photofermenter affected the flux of electrons being directed towards biosynthesis and biohydrogen generation...

Rhodopseudomonas palustris , a purple nonsulfur bacterium, is a bacterium with the properties of extraordinary metabolic versatility, carbon source diversity and metabolite diversity. Due to its biodetoxification and biodegradation properties, R. palustris has been traditionally applied in wastewater treatment and bioremediation. R. palustris is rich in various metabolites, contributing to its application in agriculture, aquaculture and livestock breeding as additives. In recent years, R. palustris has been engineered as a microbial cell factory to produce valuable chemicals, especially photofermentation of hydrogen...

This study reviews the recent advances and innovations in the application of additives to improve biomethane and biohydrogen production. Biochar, nanostructured materials, novel biopolymers, zeolites, and clays are described in terms of chemical composition, properties and impact on anaerobic digestion, dark fermentation, and photofermentation. These additives can have both a simple physical effect of microbial adhesion and growth, and a more complex biochemical impact on the regulation of key parameters for CH4 and H2 production: in this study, these effects in different experimental conditions are reviewed and described...

Biohydrogen (BioH2 ) is considered as one of the most environmentally friendly fuels and a strong candidate to meet the future demand for a sustainable source of energy. Presently, the production of BioH2 from photosynthetic organisms has raised a lot of hopes in the fuel industry. Moreover, microalgal-based BioH2 synthesis not only helps to combat current global warming by capturing greenhouse gases but also plays a key role in wastewater treatment. Hence, this manuscript provides a state-of-the-art review of the upstream and downstream BioH2 production processes...

BACKGROUND: Rhodospirillum rubrum is a purple non-sulphur bacterium that produces H2 by photofermentation of several organic compounds or by water gas-shift reaction during CO fermentation. Successful strategies for both processes have been developed in light-dependent systems. This work explores a dark fermentation bioprocess for H2 production from water using CO as the electron donor. RESULTS: The study of the influence of the stirring and the initial CO partial pressure (pCO ) demonstrated that the process was inhibited at pCO of 1...

In this study, experimental results of hydrogen producing process based on anaerobic photosynthesis using the purple non-sulfur bacterium Rhodobacter capsulatus are scrutinized. The bacterial culture was carried out in a photo-bioreactor operated in a quasi-continuous mode, using lactate as a carbon source. The method is based on the continuous stirred tank reactors (CSTR) technique to access kinetic parameters. The dynamic evolution of hydrogen production as a function of time was accurately simulated using Luedeking-Piret model and the growth of R...

Annually, approximately 1.3 billion tons of food is lost worldwide, accounting for one-third of annual food production. Therefore, turning food waste into energy is of enormous environmental significance because of its sustainable nature. Nutrients and organic acids present in food waste can be used to produce (bio)products such as biohydrogen through biological processes. However, our understanding of the production of biohydrogen from food waste through photofermentation and dark fermentation is still restricted...

Lignocellulose is a promising feedstock for biofuel production as a renewable, carbohydrate-rich and globally abundant source of biomass. However, challenges faced include environmental and/or financial costs associated with typical lignocellulose pretreatments needed to overcome the natural recalcitrance of the material before conversion to biofuel. Anaerobic fungi are a group of underexplored microorganisms belonging to the early diverging phylum Neocallimastigomycota and are native to the intricately evolved digestive system of mammalian herbivores...

There is a great interest for replacing petroleum-derived chemical processes with biological processes to obtain fuels and plastics from industrial waste. Accordingly, Rhodopseudomonas species are capable of producing hydrogen and polyhydroxybutyrate. Culture conditions for production of both hydrogen and polyhydroxybutyrate with Rhodopseudomonas pseudopalustris (DSM 123) from tequila vinasses were analyzed. The production of hydrogen using tequila vinasses was higher with respect to two synthetic media. Replacing the headspace with N2 increased the production of hydrogen with respect to Argon, while a higher concentration of polyhydroxybutyrate was achieved using Argon as compared to N2 ...

This paper provides a unique review of hydrogen production methods with wastewater treatment to depict a clean and sustainable approach. Various methods for hydrogen production from wastewaters are identified and discussed with recent details by discussing the critical challenges, opportunities, and future directions. Five main performance sectors are considered in detail for each hydrogen production method of the recent case studies, including economic, environmental, social, technical, and reliability. Eight hydrogen production methods are reviewed, including anaerobic method, photo fermentation, dark fermentation, electrolysis, electrodialysis, photocatalysis, photoelectrochemical methods, and super water gasification...

Third generation biomass, i.e. microalgae, has emerged as a promising alternative to first and second generation biomass for biohydrogen production. However, its utilization is still low at present, due to several reasons including the strong and rigidity of the microalgal cell wall that limit the hydrolysis efficiency during dark fermentation (DF) and photofermentation (PF) processes. To improve the utilization efficiency of microalgal biomass, it is crucial that important aspects related to the production of the biomass and the following processes are elaborated...