keyword
MENU ▼
Read by QxMD icon Read
search

fadR

keyword
https://www.readbyqxmd.com/read/27761152/improvement-of-free-fatty-acid-production-using-a-mutant-acyl-coa-thioesterase-i-with-high-specific-activity-in-escherichia-coli
#1
Kwang Soo Shin, Sangwoo Kim, Sung Kuk Lee
BACKGROUND: Microbial production of oleochemicals has been actively studied in the last decade. Free fatty acids (FFAs) could be converted into a variety of molecules such as industrial products, consumer products, and fuels. FFAs have been produced in metabolically engineered Escherichia coli cells expressing a signal sequence-deficient acyl-CoA thioesterase I ('TesA). Nonetheless, increasing the expression level of 'TesA seems not to be an appropriate approach to scale up FFA production because a certain ratio of each component including fatty acid synthase and 'TesA is required for optimal production of FFAs...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27466817/engineered-production-of-short-chain-fatty-acid-in-escherichia-coli-using-fatty-acid-synthesis-pathway
#2
Kamran Jawed, Anu Jose Mattam, Zia Fatma, Saima Wajid, Malik Z Abdin, Syed Shams Yazdani
Short-chain fatty acids (SCFAs), such as butyric acid, have a broad range of applications in chemical and fuel industries. Worldwide demand of sustainable fuels and chemicals has encouraged researchers for microbial synthesis of SCFAs. In this study we compared three thioesterases, i.e., TesAT from Anaerococcus tetradius, TesBF from Bryantella formatexigens and TesBT from Bacteroides thetaiotaomicron, for production of SCFAs in Escherichia coli utilizing native fatty acid synthesis (FASII) pathway and modulated the genetic and bioprocess parameters to improve its yield and productivity...
2016: PloS One
https://www.readbyqxmd.com/read/26944841/transcriptional-repression-of-the-vc2105-protein-by-vibrio-fadr-suggests-that-it-is-a-new-auxiliary-member-of-the-fad-regulon
#3
Rongsui Gao, Jingxia Lin, Han Zhang, Youjun Feng
UNLABELLED: Recently, our group along with others reported that the Vibrio FadR regulatory protein is unusual in that, unlike the prototypical fadR product of Escherichia coli, which has only one ligand-binding site, Vibrio FadR has two ligand-binding sites and represents a new mechanism for fatty acid sensing. The promoter region of the vc2105 gene, encoding a putative thioesterase, was mapped, and a putative FadR-binding site (AA CTG GTA AGA GCA CTT) was proposed. Different versions of the FadR regulatory proteins were prepared and purified to homogeneity...
May 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/26476644/improving-alkane-synthesis-in-escherichia-coli-via-metabolic-engineering
#4
Xuejiao Song, Haiying Yu, Kun Zhu
Concerns about energy security and global petroleum supply have made the production of renewable biofuels an industrial imperative. The ideal biofuels are n-alkanes in that they are chemically and structurally identical to the fossil fuels and can "drop in" to the transportation infrastructure. In this work, an Escherichia coli strain that produces n-alkanes was constructed by heterologous expression of acyl-acyl carrier protein (ACP) reductase (AAR) and aldehyde deformylating oxygenase (ADO) from Synechococcus elongatus PCC7942...
January 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/26460058/assessment-of-bacterial-acyltransferases-for-an-efficient-lipid-production-in-metabolically-engineered-strains-of-e-coli
#5
Annika Röttig, Paul Jannis Zurek, Alexander Steinbüchel
Microbially produced lipids like triacylglycerols or fatty acid ethyl esters are currently of great interest as fuel replacements or other industrially relevant compounds. They can even be produced by non-oleaginous microbes, like Escherichia coli, upon metabolic engineering. However, there is still much room for improvement regarding the yield for a competitive microbial production of lipids or biofuels. We genetically engineered E. coli by expressing fadD, fadR, pgpB, plsB and 'tesA in combination with atfA from Acinetobacter baylyi...
November 2015: Metabolic Engineering
https://www.readbyqxmd.com/read/26433722/transcriptional-regulators-of-gntr-family-in-streptomyces-coelicolor-a3-2-analysis-in-silico-and-in-vivo-of-ytra-subfamily
#6
O Tsypik, O Yushchuk, N Zaburannyi, K Flärdh, S Walker, V Fedorenko, B Ostash
Transcriptional factors of the GntR family regulate numerous physiological and morphological processes in response to the nutrient state of bacterial cells. The number of GntR transcriptional factors in genomes of soil-dwelling actinomycetes is one of the highest among bacteria, reflecting both the large size of their chromosomes and the complex ecological niche that they occupy. However, very little is known about the roles of GntRs in actinomycete biology. Here, we analyzed the genome of model actinomycete, Streptomyces coelicolor A3(2), in an attempt to gain new insights into the function of GntR family...
May 2016: Folia Microbiologica
https://www.readbyqxmd.com/read/26385696/modulation-of-fadr-binding-capacity-for-acyl-coa-fatty-acids-through-structure-guided-mutagenesis
#7
John-Paul Bacik, Chris M Yeager, Scott N Twary, Ricardo Martí-Arbona
FadR is a versatile global regulator in Escherichia coli that controls fatty acid metabolism and thereby modulates the ability of this bacterium to grow using fatty acids or acetate as the sole carbon source. FadR regulates fatty acid metabolism in response to intra-cellular concentrations of acyl-CoA lipids. The ability of FadR to bind acyl-CoA fatty acids is thus of significant interest for the engineering of biosynthetic pathways for the production of lipid-based biofuels and commodity chemicals. Based on the available crystal structure of E...
October 2015: Protein Journal
https://www.readbyqxmd.com/read/26358622/expression-of-shewanella-frigidimarina-fatty-acid-metabolic-genes-in-e-coli-by-crispr-cas9-coupled-lambda-red-recombineering
#8
Jun Xia, Ling Wang, Jian-bo Zhu, Cheng-jun Sun, Ming-gang Zheng, Li Zheng, Ying-hua Lou, Lei Shi
OBJECTIVE: To construct a clustered, regularly interspaced, short palindromic repeats (CRISPR)/cas9 system and use this system to obtain a recombinant Escherichia coli strain possessing the fatty acid metabolism genes from a lipid-rich marine bacterium. RESULTS: The fatty acid regulatory transcription factor (fadR), delta9 (Δ(9) desaturase) and acetyl-CoA carboxylase (acc) genes were cloned from Shewanella frigidimarina. The fatty acid regulatory transcription factor (fadD) and phosphoenolpyruvate carboxylase inactivated strains were used to construct the fadR/delta9 and acc knock-in strains, which are both markerless and "scar"-less, and identified the change in fatty acid composition in the recombinant strains...
January 2016: Biotechnology Letters
https://www.readbyqxmd.com/read/26151451/gntr-family-of-bacterial-transcription-factors-and-their-dna-binding-motifs-structure-positioning-and-co-evolution
#9
Inna A Suvorova, Yuri D Korostelev, Mikhail S Gelfand
The GNTR family of transcription factors (TFs) is a large group of proteins present in diverse bacteria and regulating various biological processes. Here we use the comparative genomics approach to reconstruct regulons and identify binding motifs of regulators from three subfamilies of the GNTR family, FADR, HUTC, and YTRA. Using these data, we attempt to predict DNA-protein contacts by analyzing correlations between binding motifs in DNA and amino acid sequences of TFs. We identify pairs of positions with high correlation between amino acids and nucleotides for FADR, HUTC, and YTRA subfamilies and show that the most predicted DNA-protein interactions are quite similar in all subfamilies and conform well to the experimentally identified contacts formed by FadR from E...
2015: PloS One
https://www.readbyqxmd.com/read/26147209/phytoene-desaturase-from-oryza-sativa-oligomeric-assembly-membrane-association-and-preliminary-3d-analysis
#10
Sandra Gemmecker, Patrick Schaub, Julian Koschmieder, Anton Brausemann, Friedel Drepper, Marta Rodriguez-Franco, Sandro Ghisla, Bettina Warscheid, Oliver Einsle, Peter Beyer
Recombinant phytoene desaturase (PDS-His6) from rice was purified to near-homogeneity and shown to be enzymatically active in a biphasic, liposome-based assay system. The protein contains FAD as the sole protein-bound redox-cofactor. Benzoquinones, not replaceable by molecular oxygen, serve as a final electron acceptor defining PDS as a 15-cis-phytoene (donor):plastoquinone oxidoreductase. The herbicidal PDS-inhibitor norflurazon is capable of arresting the reaction by stabilizing the intermediary FAD(red), while an excess of the quinone acceptor relieves this blockage, indicating competition...
2015: PloS One
https://www.readbyqxmd.com/read/26051676/experimental-null-method-to-guide-the-development-of-technical-procedures-and-to-control-false-positive-discovery-in-quantitative-proteomics
#11
Xiaomeng Shen, Qiang Hu, Jun Li, Jianmin Wang, Jun Qu
Comprehensive and accurate evaluation of data quality and false-positive biomarker discovery is critical to direct the method development/optimization for quantitative proteomics, which nonetheless remains challenging largely due to the high complexity and unique features of proteomic data. Here we describe an experimental null (EN) method to address this need. Because the method experimentally measures the null distribution (either technical or biological replicates) using the same proteomic samples, the same procedures and the same batch as the case-vs-contol experiment, it correctly reflects the collective effects of technical variability (e...
October 2, 2015: Journal of Proteome Research
https://www.readbyqxmd.com/read/26050090/binding-of-shewanella-fadr-to-the-faba-fatty-acid-biosynthetic-gene-implications-for-contraction-of-the-fad-regulon
#12
Huimin Zhang, Beiwen Zheng, Rongsui Gao, Youjun Feng
The Escherichia coli fadR protein product, a paradigm/prototypical FadR regulator, positively regulates fabA and fabB, the two critical genes for unsaturated fatty acid (UFA) biosynthesis. However the scenario in the other Ɣ-proteobacteria, such as Shewanella with the marine origin, is unusual in that Rodionov and coworkers predicted that only fabA (not fabB) has a binding site for FadR protein. It raised the possibility of fad regulon contraction. Here we report that this is the case. Sequence alignment of the FadR homologs revealed that the N-terminal DNA-binding domain exhibited remarkable similarity, whereas the ligand-accepting motif at C-terminus is relatively-less conserved...
September 2015: Protein & Cell
https://www.readbyqxmd.com/read/25938298/crystal-structure-of-fad35r-from-mycobacterium-tuberculosis-h37rv-in-the-apo-state
#13
Appu Kumar Singh, Babu Manjasetty, G L Balasubramani, Sukirte Koul, Abhishek Kaushik, Mary Krishna Ekka, Vijay Singh, S Kumaran
Fad35R from Mycobacterium tuberculosis binds to the promoter site of Fad35 operon and its DNA binding activities are reduced in the presence of tetracycline and palmitoyl-CoA. We resolved the crystal structure of Fad35R using single-wavelength anomalous diffraction method (SAD). Fad35R comprises canonical DNA binding domain (DBD) and ligand binding domain (LBD), but displays several distinct structural features. Two recognition helices of two monomers in the homodimer are separated by ~ 48 Å and two core triangle-shaped ligand binding cavities are well exposed to solvent...
2015: PloS One
https://www.readbyqxmd.com/read/25802297/reassessment-of-the-genetic-regulation-of-fatty-acid-synthesis-in-escherichia-coli-global-positive-control-by-the-dual-functional-regulator-fadr
#14
L My, N Ghandour Achkar, J P Viala, E Bouveret
UNLABELLED: In Escherichia coli, the FadR transcriptional regulator represses the expression of fatty acid degradation (fad) genes. However, FadR is also an activator of the expression of fabA and fabB, two genes involved in unsaturated fatty acid synthesis. Therefore, FadR plays an important role in maintaining the balance between saturated and unsaturated fatty acids in the membrane. We recently showed that FadR also activates the promoter upstream of the fabH gene (L. My, B. Rekoske, J...
June 2015: Journal of Bacteriology
https://www.readbyqxmd.com/read/25607896/the-40-residue-insertion-in-vibrio-cholerae-fadr-facilitates-binding-of-an-additional-fatty-acyl-coa-ligand
#15
Wei Shi, Gabriela Kovacikova, Wei Lin, Ronald K Taylor, Karen Skorupski, F Jon Kull
FadR is a master regulator of fatty acid metabolism and influences virulence in certain members of Vibrionaceae. Among FadR homologues of the GntR family, the Vibrionaceae protein is unusual in that it contains a C-terminal 40-residue insertion. Here we report the structure of Vibrio cholerae FadR (VcFadR) alone, bound to DNA, and in the presence of a ligand, oleoyl-CoA. Whereas Escherichia coli FadR (EcFadR) contains only one acyl-CoA-binding site in each monomer, crystallographic and calorimetric data indicate that VcFadR has two...
January 21, 2015: Nature Communications
https://www.readbyqxmd.com/read/25566241/transcription-factors-fabr-and-fadr-regulate-both-aerobic-and-anaerobic-pathways-for-unsaturated-fatty-acid-biosynthesis-in-shewanella-oneidensis
#16
Qixia Luo, Miaomiao Shi, Yedan Ren, Haichun Gao
As genes for type II fatty acid synthesis are essential to the growth of Escherichia coli, its sole (anaerobic) pathway has significant potential as a target for novel antibacterial drug, and has been extensively studied. Despite this, we still know surprisingly little about fatty acid synthesis in bacteria because this anaerobic pathway in fact is not widely distributed. In this study, we show a novel model of unsaturated fatty acid (UFA) synthesis in Shewanella, emerging human pathogens in addition to well-known metal reducers...
2014: Frontiers in Microbiology
https://www.readbyqxmd.com/read/25527627/rv0494-is-a-starvation-inducible-auto-regulatory-fadr-like-regulator-from-mycobacterium-tuberculosis
#17
Suhail Yousuf, Rajendra Angara, Vaibhav Vindal, Akash Ranjan
Fatty acid metabolism plays an important role in the survival and pathogenesis of Mycobacterium tuberculosis. Lipids are assumed to be the major source of energy during dormancy. Here, we report the characterization of a starvation-inducible, lipid-responsive transcriptional regulator, Rv0494, divergently transcribed from the Rv0493c probable operon. The striking difference in the transcriptional regulatory apparatus between mycobacteria and other well-studied organisms, such as Escherichia coli, is the organization of mycobacterial promoters...
March 2015: Microbiology
https://www.readbyqxmd.com/read/25376905/mcbr-yncc-implications-for-the-mechanism-of-ligand-and-dna-binding-by-a-bacterial-gntr-transcriptional-regulator-involved-in-biofilm-formation
#18
Dana M Lord, Ayse Uzgoren Baran, Valerie W C Soo, Thomas K Wood, Wolfgang Peti, Rebecca Page
MqsR-controlled colanic acid and biofilm regulator (McbR, also known as YncC) is the protein product of a highly induced gene in early Escherichia coli biofilm development and has been regarded as an attractive target for blocking biofilm formation. This protein acts as a repressor for genes involved in exopolysaccharide production and an activator for genes involved in stress response. To better understand the role of McbR in governing the switch from exponential growth to the biofilm state, we determined the crystal structure of McbR to 2...
November 25, 2014: Biochemistry
https://www.readbyqxmd.com/read/25311842/a-new-glimpse-of-fadr-dna-crosstalk-revealed-by-deep-dissection-of-the-e-coli-fadr-regulatory-protein
#19
Yongchang Zhang, Rongsui Gao, Huiyan Ye, Qingjing Wang, Youjun Feng
Escherichia coli (E. coli) FadR regulator plays dual roles in fatty acid metabolism, which not only represses the fatty acid degradation (fad) system, but also activates the unsaturated fatty acid synthesis pathway. Earlier structural and biochemical studies of FadR protein have provided insights into interplay between FadR protein with its DNA target and/or ligand, while the missing knowledge gap (esp. residues with indirect roles in DNA binding) remains unclear. Here we report this case through deep mapping of old E...
December 2014: Protein & Cell
https://www.readbyqxmd.com/read/25241399/substantial-improvements-in-methyl-ketone-production-in-e-coli-and-insights-on-the-pathway-from-in-vitro-studies
#20
Ee-Been Goh, Edward E K Baidoo, Helcio Burd, Taek Soon Lee, Jay D Keasling, Harry R Beller
We previously reported development of a metabolic pathway in Escherichia coli for overproduction of medium-chain methyl ketones (MK), which are relevant to the biofuel and flavor-and-fragrance industries. This MK pathway was a re-engineered version of β-oxidation designed to overproduce β-ketoacyl-CoAs and involved overexpression of the fadM thioesterase gene. Here, we document metabolic engineering modifications that have led to a MK titer of 3.4 g/L after ~45 h of fed-batch glucose fermentation and attainment of 40% of the maximum theoretical yield (the best values reported to date for MK)...
November 2014: Metabolic Engineering
keyword
keyword
58613
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"