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https://www.readbyqxmd.com/read/29050034/mir-29b-regulates-oxidative-stress-by-targeting-sirt1-in-ovarian-cancer-cells
#1
Meng Hou, Xiaohang Zuo, Chen Li, Yan Zhang, Yue Teng
BACKGROUND: Metabolic abnormalities are frequently observed in multiple malignancies including epithelial ovarian cancer (EOC), among which imbalance between generation and elimination of reactive oxygen species (ROS) plays a critical role in EOC onset and progression. Here we investigated the role of miR-29b, a well-established tumor-suppressor miRNA in metabolic regulation of EOC cells. METHODS: cell viability and apoptosis in miR-29b inhibited and over-expressed EOC cells were evaluated by CCK8 and Annexin V-FITC/PI assays...
October 19, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29049980/the-dual-role-of-poly-adp-ribose-polymerase-1-in-modulating-parthanatos-and-autophagy-under-oxidative-stress-in-rat-cochlear-marginal-cells-of-the-stria-vascularis
#2
Hong-Yan Jiang, Yang Yang, Yuan-Yuan Zhang, Zhen Xie, Xue-Yan Zhao, Yu Sun, Wei-Jia Kong
Oxidative stress is reported to regulate several apoptotic and necrotic cell death pathways in auditory tissues. Poly(ADP-ribose) polymerase-1 (PARP-1) can be activated under oxidative stress, which is the hallmark of parthanatos. Autophagy, which serves either a pro-survival or pro-death function, can also be stimulated by oxidative stress, but the role of autophagy and its relationship with parthanatos underlying this activation in the inner ear remains unknown. In this study, we established an oxidative stress model in vitro by glucose oxidase/glucose (GO/G), which could continuously generate low concentrations of H2O2 to mimic continuous exposure to H2O2 in physiological conditions, for investigation of oxidative stress-induced cell death mechanisms and the regulatory role of PARP-1 in this process...
October 7, 2017: Redox Biology
https://www.readbyqxmd.com/read/29049545/contributions-of-kinetic-energy-and-viscous-dissipation-to-airway-resistance-in-pulmonary-inspiratory-and-expiratory-airflows-in-multiscale-symmetric-airway-models-with-various-bifurcation-angles
#3
Sanghun Choi, Jiwoong Choi, Ching-Long Lin
The aim of this study was to investigate and quantify contributions of kinetic energy and viscous dissipation to airway resistance during inspiration and expiration at various flow-rates in airway models of different bifurcation angles. We employed symmetric airway models up to the 20th generation with the following five different bifurcation angles at a tracheal flow-rate of 20 L/min: 15, 25, 35, 45 and 55 degrees. Thus, a total of 10 CFD simulations for both inspiration and expiration were conducted. Furthermore, we performed an additional four simulations with tracheal flow-rate values of 10 and 40 L/min for a bifurcation angle of 35 degree to study the effect of flow-rate on inspiration and expiration...
October 10, 2017: Journal of Biomechanical Engineering
https://www.readbyqxmd.com/read/29049296/genome-wide-expression-analysis-of-salt-stressed-diploid-and-autotetraploid-paulownia-tomentosa
#4
Zhenli Zhao, Yongsheng Li, Haifang Liu, Xiaoqiao Zhai, Minjie Deng, Yanpeng Dong, Guoqiang Fan
Paulownia tomentosa is a fast-growing tree species with multiple uses. It is grown worldwide, but is native to China, where it is widely cultivated in saline regions. We previously confirmed that autotetraploid P. tomentosa plants are more stress-tolerant than the diploid plants. However, the molecular mechanism underlying P. tomentosa salinity tolerance has not been fully characterized. Using the complete Paulownia fortunei genome as a reference, we applied next-generation RNA-sequencing technology to analyze the effects of salt stress on diploid and autotetraploid P...
2017: PloS One
https://www.readbyqxmd.com/read/29047257/rice-7-hydroxymethyl-chlorophyll-a-reductase-is-involved-in-the-promotion-of-chlorophyll-degradation-and-modulates-cell-death-signaling
#5
Weilan Piao, Su-Hyun Han, Yasuhito Sakuraba, Nam-Chon Paek
The loss of green coloration via chlorophyll (Chl) degradation typically occurs during leaf senescence. To date, many Chl catabolic enzymes have been identified and shown to interact with light harvesting complex II to form a Chl degradation complex in senescing chloroplasts; this complex might metabolically channel phototoxic Chl catabolic intermediates to prevent oxidative damage to cells. The Chl catabolic enzyme 7-hydroxymethyl Chl a reductase (HCAR) converts 7-hydroxymethyl Chl a (7-HMC a) to Chl a. The rice (Oryza sativa) genome contains a single HCAR homolog (OsHCAR), but its exact role remains unknown...
October 17, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29047228/organoiridium-photosensitizers-induce-specific-oxidative-attack-on-proteins-within-cancer-cells
#6
Pingyu Zhang, Cookson K C Chiu, Huaiyi Huang, Yuko P Y Lam, Abraha Habtemariam, Thomas Malcomson, Martin J Paterson, Guy J Clarkson, Peter B O'Connor, Hui Chao, Peter J Sadler
Strongly luminescent iridium(III) complexes, [Ir(C,N)2 (S,S)](+) (1) and [Ir(C,N)2 (O,O)] (2), containing C,N (phenylquinoline), O,O (diketonate), or S,S (dithione) chelating ligands, have been characterized by X-ray crystallography and DFT calculations. Their long phosphorescence lifetimes in living cancer cells give rise to high quantum yields for the generation of (1) O2 , with large 2-photon absorption cross-sections. 2 is nontoxic to cells, but potently cytotoxic to cancer cells upon brief irradiation with low doses of visible light, and potent at sub-micromolar doses towards 3D multicellular tumor spheroids with 2-photon red light...
October 19, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29047162/baicalin-protects-h9c2-cardiomyocytes-against-hypoxia-reoxygenation-induced-apoptosis-and-oxidative-stress-through-activation-of-mitochondrial-aldehyde-dehydrogenase-2
#7
Wen-Bin Jiang, Wei Zhao, Hao Chen, You-Yang Wu, Yi Wang, Guo-Sheng Fu, Xiang-Jun Yang
Baicalin, a flavonoid glycoside separated from Scutellaria baicalensis, has cardioprotection against ischemia/reperfusion (I/R) injury. Mitochondrial aldehyde dehydrogenase 2 (ALDH2) is considered as an endogenous protective mechanism against I/R injury depending on its antioxidant and antiapoptotic characteristics. Herein, the present study demonstrate whether ALDH2 contributes to the cardioprotection of baicalin against hypoxia/reoxygenation (H/R)-inudced H9c2 cardiomyocytes injury. Our results observed that H/R treatment resulted in a significant decrease in cells viability and obvious increases in caspase-3 activity and apoptosis rate in H9c2 cells, while these alterations were evidently reversed by baicalin pretreatment...
October 19, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/29047100/mitochondrial-and-metabolic-drivers-of-pulmonary-vascular-endothelial-dysfunction-in-pulmonary-hypertension
#8
Qiujun Yu, Stephen Y Chan
Pulmonary hypertension (PH) is a deadly and increasingly prevalent vascular disease characterized by excessive pulmonary vascular remodeling and right ventricular dysfunction which leads to right heart failure, multiorgan dysfunction, and premature death. The cause of the vascular remodeling in PH remains elusive, and thus current treatments are marginally effective and prognosis of PH remains poor. Increasing evidence indicates the pathogenic importance of endothelial dysfunction in PH. However, the underlying mechanisms of such dysfunction are not well described...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29047097/from-physiological-redox-signalling-to-oxidant-stress
#9
Jeremy P T Ward
Oxidant stress is strongly associated with cardiovascular disease, including pulmonary hypertension, but antioxidant therapies have so far proven ineffective. This is partly due to a lack of understanding of the key role played by reactive oxygen species (ROS) in physiological cell signalling, and partly to the complex interrelationships between generators of ROS (e.g. mitochondria and NADPH oxidases, NOX), cellular antioxidant systems and indeed Ca(2+) signalling. At physiological levels ROS reversibly affect the function of numerous enzymes and transcription factors, most often via oxidation of specific protein thiols...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29047084/ros-signaling-in-the-pathogenesis-of-acute-lung-injury-ali-and-acute-respiratory-distress-syndrome-ards
#10
Manuela Kellner, Satish Noonepalle, Qing Lu, Anup Srivastava, Evgeny Zemskov, Stephen M Black
The generation of reactive oxygen species (ROS) plays an important role for the maintenance of cellular processes and functions in the body. However, the excessive generation of oxygen radicals under pathological conditions such as acute lung injury (ALI) and its most severe form acute respiratory distress syndrome (ARDS) leads to increased endothelial permeability. Within this hallmark of ALI and ARDS, vascular microvessels lose their junctional integrity and show increased myosin contractions that promote the migration of polymorphonuclear leukocytes (PMNs) and the transition of solutes and fluids in the alveolar lumen...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29047078/role-of-transcription-factors-in-pulmonary-artery-smooth-muscle-cells-an-important-link-to-hypoxic-pulmonary-hypertension
#11
Annarita Di Mise, Yong-Xiao Wang, Yun-Min Zheng
Hypoxia, namely a lack of oxygen in the blood, induces pulmonary vasoconstriction and vasoremodeling, which serve as essential pathologic factors leading to pulmonary hypertension (PH). The underlying molecular mechanisms are uncertain; however, pulmonary artery smooth muscle cells (PASMCs) play an essential role in hypoxia-induced pulmonary vasoconstriction, vasoremodeling, and PH. Hypoxia causes oxidative damage to DNAs, proteins, and lipids. This damage (oxidative stress) modulates the activity of ion channels and elevates the intracellular calcium concentration ([Ca(2+)]i, Ca(2+) signaling) of PASMCs...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29047037/diesel-exhaust-particles-and-the-induction-of-macrophage-activation-and-dysfunction
#12
REVIEW
Akeem O Lawal
Diesel exhaust particles (DEP) are an important component of air particulate matter, generated from the incomplete combustion of fossil fuel in diesel engines. Several epidemiological and experimental data have shown the ability of DEP to induce oxidative stress and pro-inflammatory response as mechanisms in macrophage activation and dysfunction. Macrophages are very important to immunity and immune response due to their ability to phagocyte microbes and parasites. They also respond to toxic chemicals, such as DEP, in the environment and studies have shown that their functions may be impaired by their exposure to DEP...
October 19, 2017: Inflammation
https://www.readbyqxmd.com/read/29046782/assessment-of-the-potential-for-resistance-to-antimicrobial-violet-blue-light-in-staphylococcus-aureus
#13
Rachael M Tomb, Michelle Maclean, John E Coia, Scott J MacGregor, John G Anderson
BACKGROUND: Antimicrobial violet-blue light in the region of 405 nm is emerging as an alternative technology for hospital decontamination and clinical treatment. The mechanism of action is the excitation of endogenous porphyrins within exposed microorganisms, resulting in ROS generation, oxidative damage and cell death. Although resistance to 405 nm light is not thought likely, little evidence has been published to support this. This study was designed to establish if there is potential for tolerance development, using the nosocomial pathogen Staphylococcus aureus as the model organism...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29046679/overexpression-of-npr1-in-brassica-juncea-confers-broad-spectrum-resistance-to-fungal-pathogens
#14
Sajad Ali, Zahoor A Mir, Anshika Tyagi, Hailay Mehari, Rajendra P Meena, Javaid A Bhat, Prashant Yadav, Pradeep Papalou, Sandhya Rawat, Anita Grover
Brassica juncea (Indian mustard) is a commercially important oil seed crop, which is highly affected by many biotic stresses. Among them, Alternaria leaf blight and powdery mildew are the most devastating diseases leading to huge yield losses in B. juncea around the world. In this regard, genetic engineering is a promising tool that may possibly allow us to enhance the B. juncea disease resistance against these pathogens. NPR1 (non-expressor of pathogen-related gene 1) is a bonafide receptor of salicylic acid (SA) which modulates multiple immune responses in plants especially activation of induced and systemic acquired resistance (SAR)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29046394/recombinant-human-islet-amyloid-polypeptide-forms-shorter-fibrils-and-mediates-%C3%AE-cell-apoptosis-via-generation-of-oxidative-stress
#15
Richa Dubey, Pooja Minj, Nikita Malik, Devika M Sardesai, Shruti H Kulkarni, Jhankar D Acharya, Neel S Bhavesh, Shilpy Sharma, Ashutosh Kumar
Protein aggregation play an important role in many human diseases including Alzheimer's, Parkinson's and Type 2 diabetes mellitus (T2DM). Human islet amyloid polypeptide (hIAPP) forms amyloid plaques in pancreas of T2DM subjects that are involved in deteriorating islet function and in mediating β- cell apoptosis. However, the detailed mechanism of action, structure and nature of toxic hIAPP species responsible for this effect remains elusive till date mainly due to the high cost associated with the chemical synthesis of pure peptide required for these studies...
October 18, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/29045473/identification-of-functionally-important-micrornas-from-rice-inflorescence-at-heading-stage-of-a-qdty4-1-qtl-bearing-near-isogenic-line-under-drought-conditions
#16
Boon Huat Cheah, Sudhir Jadhao, Madavan Vasudevan, Ratnam Wickneswari, Kalaivani Nadarajah
A cross between IR64 (high-yielding but drought-susceptible) and Aday Sel (drought-tolerant) rice cultivars yielded a stable line with enhanced grain yield under drought screening field trials at International Rice Research Institute. The major effect qDTY4.1 drought tolerance and yield QTL was detected in the IR77298-14-1-2-10 Backcrossed Inbred Line (BIL) and its IR87705-7-15-B Near Isogenic Line (NIL) with 93.9% genetic similarity to IR64. Although rice yield is extremely susceptible to water stress at reproductive stage, currently, there is only one report on the detection of drought-responsive microRNAs in inflorescence tissue of a Japonica rice line...
2017: PloS One
https://www.readbyqxmd.com/read/29045428/the-role-of-pka-in-the-translational-response-to-heat-stress-in-saccharomyces-cerevisiae
#17
Carla E Barraza, Clara A Solari, Irina Marcovich, Christopher Kershaw, Fiorella Galello, Silvia Rossi, Mark P Ashe, Paula Portela
Cellular responses to stress stem from a variety of different mechanisms, including translation arrest and relocation of the translationally repressed mRNAs to ribonucleoprotein particles like stress granules (SGs) and processing bodies (PBs). Here, we examine the role of PKA in the S. cerevisiae heat shock response. Under mild heat stress Tpk3 aggregates and promotes aggregation of eIF4G, Pab1 and eIF4E, whereas severe heat stress leads to the formation of PBs and SGs that contain both Tpk2 and Tpk3 and a larger 48S translation initiation complex...
2017: PloS One
https://www.readbyqxmd.com/read/29045028/biological-effects-of-er-yag-laser-irradiation-on-the-proliferation-of-primary-human-gingival-fibroblasts
#18
Sophannary Kong, Akira Aoki, Kengo Iwasaki, Koji Mizutani, Sayaka Katagiri, Tomonari Suda, Shizuko Ichinose, Mayumi Ogita, Verica Pavlic, Yuichi Izumi
We investigated the biological effects of Er:YAG laser (2,940-nm; DELight(®) , HOYA ConBio, USA) irradiation at fluences of 3.6, 4.2, 4.9, 6.3, 8.1, or 9.7 J cm(-2) at 20 or 30 Hz for 20 or 30 s on primary human gingival fibroblasts (HGFs). Irradiation at 6.3 J cm(-2) promoted maximal cell proliferation, determined by WST-8 assay and crystal violet staining, but was accompanied by lactate dehydrogenase release, on day 3 post-irradiation. Elevation of ATP level, Ki67 staining, and cyclin-A2 mRNA expression confirmed that Er:YAG affected the cell cycle and increased the number of proliferating cells...
October 17, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/29044222/rapid-generation-and-detection-of-biomimetic-oxygen-concentration-gradients-in-vitro
#19
Daud H Khan, Steven A Roberts, John Robert Cressman, Nitin Agrawal
Hypoxic regions exist within most solid tumors and often lead to altered cellular metabolism, metastasis, and drug resistance. Reliable generation and detection of biomimetic gaseous gradients in vitro is challenging due to low spatiotemporal resolution and poor longevity of gradients utilizing microfluidic techniques. Here, we present a novel and simplistic approach for producing gradients of dissolved oxygen (DO) within a lab-on-a-chip platform. Linear and stable DO gradients with high spatial resolution are established by introducing pre-gassed media into the gradient generating network...
October 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29044181/a-novel-%C3%AE-adaptin-c-myc-complex-formation-modulated-by-oxidative-stress-in-the-control-of-the-cell-cycle-in-macrophages-and-its-implication-in-atherogenesis
#20
Victor García-González, Jaime Mas-Oliva
Our study tested the proposal that c-Myc activation in macrophages is differentially carried out dependent on the intracellular oxidative state of cells and potentially associated to the process of atherogenesis. Under our experimental conditions, the generation of reactive oxygen species carried out by the presence of oxidized low density lipoproteins (oxLDL) or Gram negative bacterial lipopolysaccharides (LPS) modifies the expression of cellular adhesion molecules such as c-Abl, calcium transport proteins such as the plasma membrane Ca(2+)-ATPase (PMCA), CD47, procaspase-7, CASP7, CHOP, transcriptional activators such as c-Jun and c-Myc and molecules that participate in the process of endocytosis like α- and β-adaptin...
October 18, 2017: Scientific Reports
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