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https://www.readbyqxmd.com/read/28052400/programmed-death-1-ligand-1-and-2-expression-in-cutaneous-squamous-cell-carcinoma-and-their-relationship-with-tumor-infiltrating-dendritic-cells
#1
Qingqing Jiao, Cuiping Liu, Wenzhong Li, Wei Li, Fuming Fang, Qihong Qian, Xueguang Zhang
The Programmed Death-1 (PD-1) receptor ligands, PD-L1 and PD-L2, are costimulatory molecules that contribute to the negative regulation of T-lymphocyte activation. It is still unclear whether there is correlation between PD-L1 or PD-L2 and tumor infiltrating dendritic cells (TIDCs) in cutaneous squamous cell carcinoma (CSCC). The aim of this study was to analyze PD-L1 and PD-L2 expression and dendritic cells (DCs) infiltration in tumor tissue of CSCC patients and investigate their clinical significance. Immunohistochemical analysis was used to evaluate the expression of PD-L1, PD-L2, CD1a, and CD83 in 61 CSCC tissues...
January 3, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28031342/transcriptional-classification-and-functional-characterization-of-human-airway-macrophage-and-dendritic-cell-subsets
#2
Vineet I Patel, J Leland Booth, Elizabeth S Duggan, Steven Cate, Vicky L White, David Hutchings, Susan Kovats, Dennis M Burian, Mikhail Dozmorov, Jordan P Metcalf
The respiratory system is a complex network of many cell types, including subsets of macrophages and dendritic cells that work together to maintain steady-state respiration. Owing to limitations in acquiring cells from healthy human lung, these subsets remain poorly characterized transcriptionally and phenotypically. We set out to systematically identify these subsets in human airways by developing a schema of isolating large numbers of cells by whole-lung bronchoalveolar lavage. Six subsets of phagocytic APC (HLA-DR(+)) were consistently observed...
December 28, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28031109/-immature-dendritic-cells-phagocytosing-apoptotic-human-spleen-cells-treated-with-puva-inhibits-the-maturation-induced-by-lps
#3
Shaozhen Yang, Guosheng Du, Yuxiang Wei, Longlong Suo, Lin Zhou, Likui Feng, Jiyong Song, Zhidong Zhu, Yonggen Zheng
Objective To investigate whether lipopolysaccharide (LPS) can induce the maturation of immature dendritic cells (imDCs) which phagocytose apoptotic spleen lymphocytes. Methods Human peripheral blood mononuclear cells (PBMCs) were induced to produce DCs by interleukin 4 (IL-4) and recombinant human granulocyte macrophage colony stimulating factor (rhGM-CSF). Human spleen cells (hSPs) were isolated and treated with psoralen combined with ultraviolet A(PUVA) to obtain apoptotic PUVA-hSPs. Co-culture of imDCs with PUVA-hSPs resulted in extracorporeal photochemotherapeutic dendritic cells (ecpDCs)...
January 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28017828/effect-of-dezocine-on-il-12-and-il-10-secretion-and-lymphocyte-activation-by-culturing-dendritic-cells-from-human-umbilical-cord-blood
#4
Chang Feng, Man Feng, Ran Jiao, Dongyi Liu, Yanwu Jin, Xin Zhao, Ruixue Xiao
Dezocine has been generally utilized for pain therapy and auxiliary anesthesia. Although it has some advantages on the prevention of some anesthesia related complications, its effect on immune responses remains unclear. Our study investigated the effects of Dezocine on IL-10 and IL-12 secretion and lymphocytes activation by culturing dendritic cells (DCs), and revealed the underlying mechanism. Mononuclear cells were divided into negative control group (GN), positive control group (GP), experimental group (GD; GD5, D7, D9)...
December 22, 2016: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28005579/spontaneous-activation-of-antigen-presenting-cells-by-genes-encoding-truncated-homo-oligomerizing-derivatives-of-cd40
#5
Noam Levin, Aviad Pato, Gal Cafri, Galit Eisenberg, Tamar Peretz, Alon Margalit, Michal Lotem, Gideon Gross
The interaction between the CD40 receptor on antigen-presenting cells (APCs) and its trimeric ligand on CD4 T cells is essential for the initiation and progression of the adaptive immune response. Here we undertook to endow CD40 with the capacity to trigger spontaneous APC activation through ligand-independent oligomerization. To this end we exploited the GCN4 yeast transcriptional activator, which contains a leucine zipper DNA-binding motif that induces homophilic interactions. We incorporated GCN4 variants forming homodimers, trimers, or tetramers at the intracellular domain of human and mouse CD40 and replaced the extracellular portion with peptide-β2m or other peptide tags...
December 21, 2016: Journal of Immunotherapy
https://www.readbyqxmd.com/read/27987042/using-dendritic-cells-to-evaluate-how-burkholderia-cenocepacia-clonal-isolates-from-a-chronically-infected-cystic-fibrosis-patient-subvert-immune-functions
#6
M Guadalupe Cabral, Marília Pereira, Zélia Silva, Inês Iria, Carla Coutinho, Andreia Lopes, Isabel Sá-Correia, Paula A Videira
Infection with Burkholderia cepacia complex (Bcc) bacteria is a threat to cystic fibrosis (CF) patients, commonly leading to a fatal pneumonia, the cepacia syndrome. It causes a massive production of pro-inflammatory cytokines and leucocyte recruitment to airway epithelium without resolving infection and contributing to tissue lesion. To dissect how Bcc bacteria subvert the immune response, we developed a co-culture model with human dendritic cells (DCs) and B. cenocepacia clonal variants isolated from a chronically infected CF patient, who died with cepacia syndrome...
December 16, 2016: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/27973435/antithymocyte-globulin-induces-a-tolerogenic-phenotype-in-human-dendritic-cells
#7
Tobias Roider, Michael Katzfuß, Carina Matos, Katrin Singer, Kathrin Renner, Peter J Oefner, Katja Dettmer-Wilde, Wolfgang Herr, Ernst Holler, Marina Kreutz, Katrin Peter
Antithymocyte globulin (ATG) is used in the prevention of graft-versus-host disease during allogeneic hematopoietic stem cell transplantation. It is generally accepted that ATG mediates its immunosuppressive effect primarily via depletion of T cells. Here, we analyzed the impact of ATG-Fresenius (now Grafalon(®)) on human monocyte-derived dendritic cells (DC). ATG induced a semi-mature phenotype in DC with significantly reduced expression of CD14, increased expression of HLA-DR, and intermediate expression of CD54, CD80, CD83, and CD86...
December 11, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27965080/influence-of-platelet-rich-plasma-on-the-immune-response-of-human-monocyte-derived-dendritic-cells-and-macrophages-stimulated-with-aspergillus-fumigatus
#8
Kristin Czakai, Marcus Dittrich, Martin Kaltdorf, Tobias Müller, Sven Krappmann, Anette Schedler, Michael Bonin, Sybille Dühring, Stefan Schuster, Cornelia Speth, Günter Rambach, Hermann Einsele, Thomas Dandekar, Jürgen Löffler
Dendritic cells (DCs) and macrophages (MΦ) are critical for protection against pathogenic fungi including Aspergillus fumigatus. To analyze the role of platelets in the innate immune response, human DCs and MΦs were challenged with A. fumigatus in presence or absence of human platelet rich plasma (PRP). Gene expression analyses and functional investigations were performed. A systems biological approach was used for initial modelling of the DC - A. fumigatus interaction. DCs in a quiescent state together with different corresponding activation states were validated using gene expression data from DCs and MΦ stimulated with A...
December 9, 2016: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/27940254/apa2h1-the-first-head-domain-of-apa2-trimeric-autotransporter-adhesin-activates-mouse-bone-marrow-derived-dendritic-cells-and-immunization-with-apa2h1-protects-against-actinobacillus-pleuropneumoniae-infection
#9
Wanhai Qin, Lei Wang, Ruidong Zhai, Qiuyue Ma, Jianfang Liu, Chuntong Bao, Diangang Sun, Hu Zhang, Changjiang Sun, Xin Feng, Jingmin Gu, Chongtao Du, Wenyu Han, P R Langford, Liancheng Lei
Actinobacillus pleuropneumoniae is the causative pathogen of porcine pleuropneumonia, which results in large economic losses in the pig industry worldwide. There are, however, no effective subunit vaccines are available in the market owing to the various serotypes and the absence of cross-protection against this pathogen. Therefore, the selection of protective components is of great significance for vaccine development. We previously showed that trimeric autotransporter adhesins are important virulence factors of A...
January 2017: Molecular Immunology
https://www.readbyqxmd.com/read/27932984/curcumin-suppressed-activation-of-dendritic-cells-via-jak-stat-socs-signal-in-mice-with-experimental-colitis
#10
Hai-Mei Zhao, Rong Xu, Xiao-Ying Huang, Shao-Min Cheng, Min-Fang Huang, Hai-Yang Yue, Xin Wang, Yong Zou, Ai-Ping Lu, Duan-Yong Liu
Dendritic cells (DCs) play a pivotal role as initiators in the pathogenesis of inflammatory bowel disease and are regulated by the JAK/STAT/SOCS signaling pathway. As a potent anti-inflammatory compound, curcumin represents a viable treatment alternative or adjunctive therapy in the management of chronic inflammatory bowel disease (IBD). The mechanism of curcumin treated IBD on DCs is not completely understood. In the present study, we explored the mechanism of curcumin treated experimental colitis by observing activation of DCs via JAK/STAT/SOCS signaling pathway in colitis mice...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27895173/human-regulatory-t-cells-mediate-transcriptional-modulation-of-dendritic-cell-function
#11
Emily Mavin, Lindsay Nicholson, Syed Rafez Ahmed, Fei Gao, Anne Dickinson, Xiao-Nong Wang
Regulatory T cells (Treg) attenuate dendritic cell (DC) maturation and stimulatory function. Current knowledge on the functional impact of semimature DC is limited to CD4(+) T cell proliferation and cytokine production. Little is known about the molecular basis underpinning the functional effects of Treg-treated DC (Treg-DC). We present novel evidence that Treg-DC skewed CD4(+) naive T cell polarization toward a regulatory phenotype and impaired CD8(+) T cell allo-reactive responses, including their ability to induce target tissue damage in a unique in vitro human graft-versus-host disease skin explant model...
January 1, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27882218/adenovirus-co-expressing-cd40-ligand-and-interleukin-il-2-contributes-to-maturation-of-dendritic-cells-and-production-of-il-12
#12
Zhi Guo, Hong-Yan Gao, Tian-Yang Zhang, Jin-Xing Lou, Kai Yang, Xiao-Dong Liu, Xue-Peng He, Hui-Ren Chen
The aim of the present study was to construct a chimeric adenovirus (Ad)5/F35 co-expressing human CD4O ligand (CD4OL) and interleukin (IL)-2 (Ad5/F35 CD40L-IL-2). The infection efficiency to human monocyte-derived dendritic cells (Mo-DCs), expression of genes, phenotype changes and IL-12 production of Mo-DC by Ad5/F35 CD40L-IL-2 were investigated. CD40L and IL-2 from total RNA extracted from human peripheral blood mononuclear cells (PBMCs) were cloned by reverse transcription-polymerase chain reaction and used to construct Ad5/F35 CD40L-IL-2...
November 2016: Biomedical Reports
https://www.readbyqxmd.com/read/27837105/the-analysis-of-cd83-expression-on-human-immune-cells-identifies-a-unique-cd83-activated-t-cell-population
#13
Xinsheng Ju, Pablo A Silveira, Wei-Hsun Hsu, Zehra Elgundi, Renz Alingcastre, Nirupama D Verma, Phillip D Fromm, Jennifer L Hsu, Christian Bryant, Ziduo Li, Fiona Kupresanin, Tsun-Ho Lo, Candice Clarke, Kenneth Lee, Helen McGuire, Barbara Fazekas de St Groth, Stephen R Larsen, John Gibson, Kenneth F Bradstock, Georgina J Clark, Derek N J Hart
CD83 is a member of the Ig gene superfamily, first identified in activated lymphocytes. Since then, CD83 has become an important marker for defining activated human dendritic cells (DC). Several potential CD83 mRNA isoforms have been described, including a soluble form detected in human serum, which may have an immunosuppressive function. To further understand the biology of CD83, we examined its expression in different human immune cell types before and after activation using a panel of mouse and human anti-human CD83 mAb...
December 15, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27832135/intestinal-dendritic-cells-are-altered-in-number-maturity-and-chemotactic-ability-in-fulminant-hepatic-failure
#14
Mei Liu, Peng Wang, Min Zhao, D Y Liu
Fulminant hepatic failure (FHF) is defined as rapid acute liver injury, often complicated with spontaneous bacterial peritonitis (SBP). The precise onset of FHF with SBP is still unknown, but it is thought that SBP closely correlates with a weakened intestinal barrier. Dendritic cells (DCs) play a crucial role in forming the intestinal immune barrier, therefore the number, maturity and chemotactic ability of intestinal DCs were studied in FHF. Mouse intestinal and spleen DCs were isolated by magnetic-activated cell sorting (MACS) and surface markers of DCs, namely CD11c, CD74, CD83 and CD86, were identified using flow cytometry...
2016: PloS One
https://www.readbyqxmd.com/read/27825104/human-15-lipoxygenase-1-is-a-regulator-of-dendritic-cell-spreading-and-podosome-formation
#15
Hongya Han, Xiuming Liang, Monica Ekberg, Joanna S Kritikou, Åsa Brunnström, Benjamin Pelcman, Maria Matl, Xinyan Miao, Margareta Andersson, Xiaotian Yuan, Frida Schain, Selina Parvin, Eva Melin, Jan Sjöberg, Dawei Xu, Lisa S Westerberg, Magnus Björkholm, Hans-Erik Claesson
Dendritic cells (DCs) involved in proinflammatory immune responses derive mainly from peripheral monocytes, and the cells subsequently mature and migrate into the inflammatory micromilieu. Here we report that suppressing of 15-lipoxygenase-1 led to a substantial reduction in DC spreading and podosome formation in vitro. The surface expression of CD83 was significantly lower in both sh-15-lipoxygenase-1 (15-LOX-1)-transduced cells and DCs cultivated in the presence of the inhibitor. The T-cell response against tetanus-pulsed DCs was only affected to a minor extent on inhibition of 15-LOX-1...
October 17, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27823917/alternaria-alternata-acts-on-human-monocyte-derived-dendritic-cells-to-mediate-th2-th17-polarisation
#16
A Loghmani, R Raoofi, A Ownagh, N Delirezh
INTRODUCTION: Although the mechanism of asthma is not precisely understood in humans, clinical and epidemiological studies have offered a potential relationship between exposure to environmental fungi, such as Alternaria alternata (A. alternata) and the development and exacerbation of asthma. The aim of this project is to investigate the mechanisms of Th2 responses by A. alternata as a clinically relevant model for the environmental exposure. MATERIALS AND METHODS: Plastic adherent monocytes were cultured with granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) to convert these cells into Monocyte-derived Dendritic cells (MoDc) and then matured in the presence of Monocyte-Conditioned Medium (MCM) as the control group and MCM+ A...
November 4, 2016: Allergologia et Immunopathologia
https://www.readbyqxmd.com/read/27815198/comparative-immunity-of-salmo-salar-and-oncorhynchus-kisutch-during-infestation-with-the-sea-louse-caligus-rogercresseyi-an-enrichment-transcriptome-analysis
#17
Valentina Valenzuela-Muñoz, Sebastian Boltaña, Cristian Gallardo-Escárate
Caligus rogercresseyi, an ectoparasite affecting the Chilean salmon industry, can cause immunosuppression and physiological stress in farmed fish. Interestingly, coho salmon (Oncorhynchus kisutch) are notably resistant to infestation, whereas Atlantic salmon (Salmo salar) are phenotypically more susceptible to sea lice. However, comparative studies on immune responses to C. rogercresseyi have not been conducted. In this study, Illumina sequencing was conducted to evaluate head kidney and skin samples taken 7 and 14 days post-infestation, yielding a total of 1492 and 1522 contigs annotated to immune-related genes for Atlantic and coho salmon, respectively...
December 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27812247/chronic-myelocytic-leukemia-cml-patient-derived-dendritic-cells-transfected-with-autologous-total-rna-induces-cml-specific-cytotoxicity
#18
Li Yu, Ting Hu, Tian Zou, Qingzhi Shi, Guoan Chen
The oncogenic bcr/abl1 fusion gene is a chronic myelogenous leukemia (CML)-specific antigen which is absent in normal tissues. This makes bcr/abl1 a perfect target for developing CML vaccines that elicit specific immune responses against minimal residual disease while sparing normal tissue. The aim of this study was to use different methods to induce dendritic cells (DCs) derived from patients with CML (CML-DCs) and analyze them for CML-specific tumor cytotoxicity for immune therapy. Bone marrow-derived mononuclear cells from ten CML patients were studied to induce CML-DC differentiation in the presence of recombinant human interleukin-4, rh-granulocyte-macrophage-colony stimulating factor, and tumor necrosis factor-alpha with either a total RNA-lipofectamine complex, total RNA or CML tumor lysate (freeze-thawed)...
December 2016: Indian Journal of Hematology & Blood Transfusion
https://www.readbyqxmd.com/read/27730669/highly-pathogenic-avian-influenza-h5n1-virus-induces-cytokine-dysregulation-with-suppressed-maturation-of-chicken-monocyte-derived-dendritic-cells
#19
Semmannan Kalaiyarasu, Manoj Kumar, Dhanapal Senthil Kumar, Sandeep Bhatia, Sandeep Kumar Dash, Sushant Bhat, Rohit K Khetan, Shanmugasundaram Nagarajan
One of the major causes of death in highly pathogenic avian influenza virus (HPAIV) infection in chickens is acute induction of pro-inflammatory cytokines (cytokine storm), which leads to severe pathology and acute mortality. DCs and respiratory tract macrophages are the major antigen presenting cells that are exposed to mucosal pathogens. We hypothesized that chicken DCs are a major target for induction of cytokine dysregulation by H5N1 HPAIV. It was found that infection of chicken peripheral blood monocyte-derived dendritic cells (chMoDCs) with H5N1 HPAIV produces high titers of progeny virus with more rounding and cytotoxicity than with H9N2 LPAIV...
October 2016: Microbiology and Immunology
https://www.readbyqxmd.com/read/27723792/effect-of-cryopreserved-amniotic-membrane-orientation-on-the-expression-of-limbal-mesenchymal-and-epithelial-stem-cell-markers-in-prolonged-limbal-explant-cultures
#20
Zala Lužnik, Marko Hawlina, Elvira Maličev, Marina Bertolin, Andreja Nataša Kopitar, Alojz Ihan, Stefano Ferrari, Petra Schollmayer
PURPOSE: To evaluate the effect of prolonged limbal explants cultured without any scaffolds or on amniotic membrane (AM) on the viability, proliferation and differentiation potential of putative phenotypically defined cultured limbal mesenchymal (LMSC) and epithelial stem cells (LESC). METHODS: Limbal explants were cultivated on cryopreserved intact AM or plastic plates using medium supplemented with only human serum. AM was positioned with either the epithelial or stromal side up...
2016: PloS One
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