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https://www.readbyqxmd.com/read/28819897/teachers-and-parents-perspectives-on-a-curricular-subject-of-religion-and-spirituality-for-indian-schools-a-pilot-study-toward-school-mental-health-program
#1
Parameshwaran Ramakrishnan, Andrew Baccari, Uma Ramachandran, Syed Faiz Ahmed, Harold G Koenig
Religious-spiritual (R/S) education helps medical students cope with caregiving stress and gain skills in interpersonal empathy needed for clinical care. Such R/S education has been introduced into K-12 and college curricula in some developed nations and has been found to positively impact student's mental health. Such a move has not yet been seen in the Indian education system. This paper aimed to examine perspectives of teachers and parents in India on appropriateness, benefits, and challenges of including R/S education into the school curriculum and also to gather their impressions on how a R/S curriculum might promote students' health...
August 17, 2017: Journal of Religion and Health
https://www.readbyqxmd.com/read/28818744/cytotoxic-genotoxic-and-antimicrobial-activity-of-caffeic-and-rosmarinic-acids-and-their-lithium-sodium-and-potassium-salts-as-potential-anticancer-compounds
#2
Marzena Matejczyk, Renata Świsłocka, Aleksandra Golonko, Włodzimierz Lewandowski, Eliza Hawrylik
PURPOSE: The aim of this study was to examine the cytotoxic, genotoxic, antioxidant and antimicrobial activity of caffeic and rosmarinic acids and their salts with Li, Na and K with use of Escherichia coli K-12 recA:gfp strain as a model organism. METHODS: Cytotoxic potency of tested chemicals were calculated on the basis on the dose that confers inhibition percentage such as 20% for each concentrations of analysed chemicals. Genotoxic properties were calculated on the basis of the fold increase (FI) of SFI values normalized with control...
August 14, 2017: Advances in Medical Sciences
https://www.readbyqxmd.com/read/28816485/risks-resources-and-depressive-symptomatology-among-marshallese-adolescents
#3
Kevin M Fitzpatrick, Don Willis
This study examined risks and resources to assess depressive symptom variation among a sample of Marshallese adolescents attending a Northwest Arkansas high school. A self-administered questionnaire was completed by 10th-12th-grade students (N = 1,493). With a general response rate in excess of 78%, the subsample (n = 208) represented 75% of all Marshallese students attending the high school and approximately 9% of the total K-12 Marshallese population. Average depressive symptom (CES-D) scores for Marshallese adolescents were 17...
August 17, 2017: American Journal of Orthopsychiatry
https://www.readbyqxmd.com/read/28799833/plasmid-mediated-colistin-resistance-gene-mcr-1-in-an-escherichia-coli-st10-bloodstream-isolate-in-the-sultanate-of-oman
#4
Jalila Mohsin, Tibor Pál, Jorgen Eskild Petersen, Dania Darwish, Akela Ghazawi, Tanveer Ashraf, Agnes Sonnevend
AIMS: To identify plasmid-mediated colistin resistance in clinical Enterobacteriaceae isolates in Oman, where this resistance mechanism has not been encountered yet. MATERIALS/METHODS: Twenty-two colistin-resistant Enterobacteriaceae clinical isolates collected between July 2014 and June 2016 in a tertiary care hospital in Muscat were screened by PCR for the mcr-1 and mcr-2 genes. The strain identified as mcr-1 positive was genotyped and its antibiotic susceptibility was established...
August 11, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28794860/minion-analysis-and-reference-consortium-phase-2-data-release-and-analysis-of-r9-0-chemistry
#5
Miten Jain, John R Tyson, Matthew Loose, Camilla L C Ip, David A Eccles, Justin O'Grady, Sunir Malla, Richard M Leggett, Ola Wallerman, Hans J Jansen, Vadim Zalunin, Ewan Birney, Bonnie L Brown, Terrance P Snutch, Hugh E Olsen
BACKGROUND: Long-read sequencing is rapidly evolving and reshaping the suite of opportunities for genomic analysis. For the MinION in particular, as both the platform and chemistry develop, the user community requires reference data to set performance expectations and maximally exploit third-generation sequencing. We performed an analysis of MinION data derived from whole genome sequencing of Escherichiacoli K-12 using the R9.0 chemistry, comparing the results with the older R7.3 chemistry...
2017: F1000Research
https://www.readbyqxmd.com/read/28782004/second-flexner-century-the-democratization-of-medical-knowledge-repurposing-a-general-pathology-course-into-multigrade-level-gateway-courses
#6
Ronald S Weinstein, Amy L Waer, John B Weinstein, Margaret M Briehl, Michael J Holcomb, Kristine A Erps, Angelette L Holtrust, Julie M Tomkins, Gail P Barker, Elizabeth A Krupinski
Starting in 1910, the "Flexner Revolution" in medical education catalyzed the transformation of the US medical education enterprise from a proprietary medical school dominated system into a university-based medical school system. In the 21st century, what we refer to as the "Second Flexner Century" shifts focus from the education of medical students to the education of the general population in the "4 health literacies." Compared with the remarkable success of the first Flexner Revolution, retrofitting medical science education into the US general population today, starting with K-12 students, is a more daunting task...
January 2017: Acad Pathol
https://www.readbyqxmd.com/read/28774286/development-of-a-new-fluorescent-reporter-operator-system-location-of-arac-regulated-genes-in-escherichia-coli-k-12
#7
Laura E Sellars, Jack A Bryant, María-Antonia Sánchez-Romero, Eugenio Sánchez-Morán, Stephen J W Busby, David J Lee
BACKGROUND: In bacteria, many transcription activator and repressor proteins regulate multiple transcription units that are often distally distributed on the bacterial genome. To investigate the subcellular location of DNA bound proteins in the folded bacterial nucleoid, fluorescent reporters have been developed which can be targeted to specific DNA operator sites. Such Fluorescent Reporter-Operator System (FROS) probes consist of a fluorescent protein fused to a DNA binding protein, which binds to an array of DNA operator sites located within the genome...
August 3, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28766320/nutrition-education-and-body-mass-index-in-grades-k-12-a-systematic-review
#8
Cayla Price, Deborah Cohen, Peter Pribis, Jean Cerami
BACKGROUND: Overweight and obese body mass index (BMI) status affects an increasing number of children in the United States. The school setting has been identified as a focus area to implement obesity prevention programs. METHODS: A database search of PubMed, Education Search Complete, and Cumulative Index to Nursing and Allied Health Literature (CINAHL) was conducted for peer-reviewed articles published between January 2005 and December 2015 pertaining to programs offered in the school setting to grades K-12 in the United States with a nutrition education component and measured BMI percentile or BMI z-score as an outcome...
September 2017: Journal of School Health
https://www.readbyqxmd.com/read/28745488/fabrication-of-tio2-reduced-graphene-oxide-nanorod-composition-spreads-using-combinatorial-hydrothermal-synthesis-and-their-photocatalytic-and-photoelectrochemical-applications
#9
Wen-Chung Lu, Li-Chun Tseng, Kao-Shuo Chang
This study is the first to employ combinatorial hydrothermal synthesis and facile spin-coating technology to fabricate TiO2-reduced graphene oxide (rGO) nanorod composition spreads. The features of this study are (1) the development of a self-designed spin-coating wedge, (2) the systemic investigation of the structure-property relationship of the system, (3) the high-throughput screening of the optimal ratio from a wide range of compositions for photocatalytic and photoelectrochemical (PEC) applications, and (4) the effective coupling between the density gradient TiO2 nanorod array and the thickness gradient rGO...
August 8, 2017: ACS Combinatorial Science
https://www.readbyqxmd.com/read/28733283/osmotic-compounds-enhance-antibiotic-efficacy-against-acinetobacter-baumannii-biofilm-communities
#10
Azeza Falghoush, Haluk Beyenal, Thomas E Besser, Anders Omsland, Douglas R Call
Biofilm-associated infections are a clinical challenge in part because a hydrated matrix protects the bacterial community from antibiotics. Herein, we evaluated how different osmotic compounds [maltodextrin, sucrose and polyethylene glycol (PEG)] enhance antibiotic efficacy against Acinetobacter baumannii biofilm communities. Established test-tube biofilms (24 h, strain ATCC 17978) were treated with osmotic compounds in the presence or absence of 10X the minimum inhibitory concentration of different antibiotics (50 μg/ml tobramycin, 20 μg/ml ciprofloxacin, 300 μg/ml chloramphenicol, 30 μg/ml nalidixic acid or 100 μg/ml erythromycin)...
July 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28727911/ultrasound-processing-of-liquid-system-s-and-its-antimicrobial-mechanism-of-action
#11
David Spiteri, Claire Chot-Plassot, Julie Sclear, Kimon Andreas Karatzas, Chris Scerri, Vasilis Valdramidis
Ultrasound creates cavitation phenomena, resulting in the formation of several free radicals, namely OH• and H•, due to the breakdown of the H2 O molecule. These radicals affect the cellular integrity of the bacteria, causing the inactivation of several processes, and thus it is important to unravel the mechanism of action of this technology. This research looks into the application and mechanism of action of ultrasound technology as a means of disinfection by acoustic cavitation. Sterile water and synthetic waste water were inoculated with different mutants of E...
July 20, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28699876/characterization-of-vsr-endonucleases-from-neisseria-meningitidis
#12
Milena Bażlekowa, Monika Adamczyk-Popławska, Agnieszka Kwiatek
DNA methylation is a common modification occurring in all living organisms. 5-methylcytosine, which is produced in a reaction catalysed by C5-methyltransferases, can spontaneously undergo deamination to thymine, leading to the formation of T:G mismatches and C→T transitions. In Escherichia coli K-12, such mismatches are corrected by the Very Short Patch (VSP) repair system, with Vsr endonuclease as the key enzyme. Neisseria meningitidis possesses genes that encode DNA methyltransferases, including C5-methyltransferases...
July 12, 2017: Microbiology
https://www.readbyqxmd.com/read/28684575/draft-genome-sequence-of-escherichia-coli-k-12-atcc-29425
#13
Kathleen C Engelbrecht, Catherine Putonti, David W Koenig, Alan J Wolfe
A draft genome sequence for Escherichia coli ATCC 29425 was investigated. The size of the genome was 4,608,319 bp, with an observed G+C content of 50.68%. This assembly consisted of 80 contigs, with an average coverage of 122.2×, including one contig representative of the complete genome for the temperate phage P1.
July 6, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28684574/draft-genome-sequence-of-escherichia-coli-k-12-atcc-10798
#14
Daniela Dimitrova, Kathleen C Engelbrecht, Catherine Putonti, David W Koenig, Alan J Wolfe
Here, we present the draft genome sequence of Escherichia coli ATCC 10798. E. coli ATCC 10798 is a K-12 strain, one of the most well-studied model microorganisms. The size of the genome was 4,685,496 bp, with a G+C content of 50.70%. This assembly consists of 62 contigs and the F plasmid.
July 6, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28681361/top-down-lesa-mass-spectrometry-protein-analysis-of-gram-positive-and-gram-negative-bacteria
#15
Klaudia I Kocurek, Leanne Stones, Josephine Bunch, Robin C May, Helen J Cooper
We have previously shown that liquid extraction surface analysis (LESA) mass spectrometry (MS) is a technique suitable for the top-down analysis of proteins directly from intact colonies of the Gram-negative bacterium Escherichia coli K-12. Here we extend the application of LESA MS to Gram-negative Pseudomonas aeruginosa PS1054 and Gram-positive Staphylococcus aureus MSSA476, as well as two strains of E. coli (K-12 and BL21 mCherry) and an unknown species of Staphylococcus. Moreover, we demonstrate the discrimination between three species of Gram-positive Streptococcus (Streptococcus pneumoniae D39, and the viridans group Streptococcus oralis ATCC 35037 and Streptococcus gordonii ATCC35105), a recognized challenge for matrix-assisted laser desorption ionization time-of-flight MS...
July 5, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28679572/developing-a-nationwide-k-12-outreach-model-physiology-understanding-phun-week-10-years-later
#16
Margaret Stieben, Patricia A Halpin, Marsha Lakes Matyas
Since 2005, nearly 600 Physiology Understanding Week (PhUn Week) events have taken place across the U.S., involving American Physiological Society (APS) members in K-12 outreach. The program seeks to build student understanding of physiology and physiology careers, assist teachers in recognizing physiology in their standards-based curriculum, and involve more physiologists in K-12 outreach. Formative goals included program growth (sites, participants, and leaders), diversification of program models, and development of a community of practice of physiologists and trainees involved in outreach...
September 1, 2017: Advances in Physiology Education
https://www.readbyqxmd.com/read/28671706/an-integrative-machine-learning-strategy-for-improved-prediction-of-essential-genes-in-escherichia-coli-metabolism-using-flux-coupled-features
#17
Sutanu Nandi, Abhishek Subramanian, Ram Rup Sarkar
Prediction of essential genes helps to identify a minimal set of genes that are absolutely required for the appropriate functioning and survival of a cell. The available machine learning techniques for essential gene prediction have inherent problems, like imbalanced provision of training datasets, biased choice of the best model for a given balanced dataset, choice of a complex machine learning algorithm, and data-based automated selection of biologically relevant features for classification. Here, we propose a simple support vector machine-based learning strategy for the prediction of essential genes in Escherichia coli K-12 MG1655 metabolism that integrates a non-conventional combination of an appropriate sample balanced training set, a unique organism-specific genotype, phenotype attributes that characterize essential genes, and optimal parameters of the learning algorithm to generate the best machine learning model (the model with the highest accuracy among all the models trained for different sample training sets)...
July 25, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28666008/the-whole-set-of-the-constitutive-promoters-recognized-by-four-minor-sigma-subunits-of-escherichia-coli-rna-polymerase
#18
Tomohiro Shimada, Kan Tanaka, Akira Ishihama
The promoter selectivity of Escherichia coli RNA polymerase (RNAP) is determined by the sigma subunit. The model prokaryote Escherichia coli K-12 contains seven species of the sigma subunit, each recognizing a specific set of promoters. For identification of the "constitutive promoters" that are recognized by each RNAP holoenzyme alone in the absence of other supporting factors, we have performed the genomic SELEX screening in vitro for their binding sites along the E. coli K-12 W3110 genome using each of the reconstituted RNAP holoenzymes and a collection of genome DNA segments of E...
2017: PloS One
https://www.readbyqxmd.com/read/28663823/sequencing-a-piece-of-history-complete-genome-sequence-of-the-original-escherichia-coli-strain
#19
Karl A Dunne, Roy R Chaudhuri, Amanda E Rossiter, Irene Beriotto, Douglas F Browning, Derrick Squire, Adam F Cunningham, Jeffrey A Cole, Nicholas Loman, Ian R Henderson
In 1885, Theodor Escherich first described the Bacillus coli commune, which was subsequently renamed Escherichia coli. We report the complete genome sequence of this original strain (NCTC 86). The 5 144 392 bp circular chromosome encodes the genes for 4805 proteins, which include antigens, virulence factors, antimicrobial-resistance factors and secretion systems, of a commensal organism from the pre-antibiotic era. It is located in the E. coli A subgroup and is closely related to E. coli K-12 MG1655. E...
March 2017: Microbial Genomics
https://www.readbyqxmd.com/read/28654677/ecoblmcrx-a-classical-modification-dependent-restriction-enzyme-in-escherichia-coli-b-characterization-in-vivo-and-in-vitro-with-a-new-approach-to-cleavage-site-determination
#20
Alexey Fomenkov, Zhiyi Sun, Deborah K Dila, Brian P Anton, Richard J Roberts, Elisabeth A Raleigh
Here we characterize the modification-dependent restriction enzyme (MDE) EcoBLMcrX in vivo, in vitro and in its genomic environment. MDE cleavage of modified DNAs protects prokaryote populations from lethal infection by bacteriophage with highly modified DNA, and also stabilizes lineages by reducing gene import when sparse modification occurs in the wrong context. The function and distribution of MDE families are thus important. Here we describe the properties of EcoBLMcrX, an enzyme of the E. coli B lineage, in vivo and in vitro...
2017: PloS One
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