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https://www.readbyqxmd.com/read/27917489/nutrition-quality-of-us-school-snack-foods-a-first-look-at-2011-2014-bid-records-in-8-school-districts
#1
Y Claire Wang, Amber Hsiao, Peter Chamberlin, McKenzie Largay, Abbie Archibald, Andrew Malone, JoAnn Stevelos
BACKGROUND: As part of the Healthy, Hunger-Free Kids Act, snacks, and desserts sold in K-12 schools as of the 2014-2015 school year are required to meet the "Smart Snacks" nutritional guidelines. Although studies exist in tracking progress in local and national efforts, the proportion of snack food procured by school districts compliant with the Smart Snacks standard prior to its full implementation is unknown. METHODS: We repurposed a previously untapped database, Interflex, of public bid records to examine the nutritional quality of snacks and desserts procured by school districts...
January 2017: Journal of School Health
https://www.readbyqxmd.com/read/27917101/selective-enzymatic-transformation-to-aldehydes-in-vivo-by-fungal-carboxylate-reductase-from-neurospora-crassa
#2
Daniel Schwendenwein, Giuseppe Fiume, Hansjörg Weber, Florian Rudroff, Margit Winkler
The enzymatic reduction of carboxylic acids is in its infancy with only a handful of biocatalysts available to this end. We have increased the spectrum of carboxylate-reducing enzymes (CARs) with the sequence of a fungal CAR from Neurospora crassa OR74A (NcCAR). NcCAR was efficiently expressed in E. coli using an autoinduction protocol at low temperature. It was purified and characterized in vitro, revealing a broad substrate acceptance, a pH optimum at pH 5.5-6.0, a Tm of 45 °C and inhibition by the co-product pyrophosphate which can be alleviated by the addition of pyrophosphatase...
November 3, 2016: Advanced Synthesis & Catalysis
https://www.readbyqxmd.com/read/27909030/the-college-science-learning-cycle-an-instructional-model-for-reformed-teaching
#3
Michelle Withers
Finding the time for developing or locating new class materials is one of the biggest barriers for instructors reforming their teaching approaches. Even instructors who have taken part in training workshops may feel overwhelmed by the task of transforming passive lecture content to engaging learning activities. Learning cycles have been instrumental in helping K-12 science teachers design effective instruction for decades. This paper introduces the College Science Learning Cycle adapted from the popular Biological Sciences Curriculum Study 5E to help science, technology, engineering, and mathematics faculty develop course materials to support active, student-centered teaching approaches in their classrooms...
2016: CBE Life Sciences Education
https://www.readbyqxmd.com/read/27901648/a-new-drug-design-strategy-killing-drug-resistant-bacteria-by-deactivating-their-hypothetical-genes
#4
Tit-Yee Wong, Jimmy Kuo
Despite a bacterial genome is complicated by large numbers of horizontally transferred (HT) genes and function unknown hypothetical (FUN) genes, the Genic-Transcriptional-Stop-Signals-Ratio (TSSR) of a genome shows that HT and FUN genes are complementary to all other genes in the genome. When HT or certain FUN genes are omitted from the Escherichia coli K-12 genome, its Genomic-TSSR value becomes totally incomparable to other E. coli strains. The Genic-TSSR correlation tree of a pathogen shows that some FUN genes would form a unique cluster...
November 30, 2016: Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews
https://www.readbyqxmd.com/read/27899573/the-ecocyc-database-reflecting-new-knowledge-about-escherichia-coli-k-12
#5
Ingrid M Keseler, Amanda Mackie, Alberto Santos-Zavaleta, Richard Billington, César Bonavides-Martínez, Ron Caspi, Carol Fulcher, Socorro Gama-Castro, Anamika Kothari, Markus Krummenacker, Mario Latendresse, Luis Muñiz-Rascado, Quang Ong, Suzanne Paley, Martin Peralta-Gil, Pallavi Subhraveti, David A Velázquez-Ramírez, Daniel Weaver, Julio Collado-Vides, Ian Paulsen, Peter D Karp
EcoCyc (EcoCyc.org) is a freely accessible, comprehensive database that collects and summarizes experimental data for Escherichia coli K-12, the best-studied bacterial model organism. New experimental discoveries about gene products, their function and regulation, new metabolic pathways, enzymes and cofactors are regularly added to EcoCyc. New SmartTable tools allow users to browse collections of related EcoCyc content. SmartTables can also serve as repositories for user- or curator-generated lists. EcoCyc now supports running and modifying E...
November 28, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27884783/secondary-multidrug-efflux-pump-mutants-alter-escherichia-coli-biofilm-growth-in-the-presence-of-cationic-antimicrobial-compounds
#6
Denice C Bay, Carol A Stremick, Carmine J Slipski, Raymond J Turner
Escherichia coli possesses many secondary active multidrug resistance transporters (MDTs) that confer overlapping substrate resistance to a broad range of antimicrobials via proton and/or sodium motive force. It is uncertain whether redundant MDTs uniquely alter cell survival when cultures grow planktonically or as biofilms. In this study, the planktonic and biofilm growth and antimicrobial resistance of 13 E. coli K-12 single MDT gene deletion strains in minimal and rich media were determined. Antimicrobial tolerance to tetracycline, tobramycin and benzalkonium were also compared for each ΔMDT strain...
November 22, 2016: Research in Microbiology
https://www.readbyqxmd.com/read/27872391/the-role-of-school-health-services-in-addressing-the-needs-of-students-with-chronic-health-conditions-a-systematic-review
#7
Zanie C Leroy, Robin Wallin, Sarah Lee
Children and adolescents in the United States spend many hours in school. Students with chronic health conditions (CHCs) may face lower academic achievement, increased disability, fewer job opportunities, and limited community interactions as they enter adulthood. School health services provide safe and effective management of CHCs, often for students with limited access to health care. A systematic review to assess the role of school health services in addressing CHCs among students in Grades K-12 was completed using primary, peer-reviewed literature published from 2000 to 2015, on selected conditions: asthma, food allergies, diabetes, seizure disorders, and poor oral health...
November 20, 2016: Journal of School Nursing: the Official Publication of the National Association of School Nurses
https://www.readbyqxmd.com/read/27866385/effectiveness-of-universal-school-based-mental-health-awareness-programs-among-youth-in-the-united-states-a-systematic-review
#8
John P Salerno
BACKGROUND: Stigmatizing attitudes toward mental illness and low mental health literacy have been identified as links to social adversity, and barriers to seeking and adhering to treatment among adolescents suffering from mental illness. Prior research has found that it is possible to improve these outcomes using school-based mental health awareness interventions. The purpose of this study was to review empirical literature pertaining to universal mental health awareness interventions aiming to improve mental health related outcomes among students enrolled in US K-12 schools, especially minorities vulnerable to health disparities...
December 2016: Journal of School Health
https://www.readbyqxmd.com/read/27842452/role-of-bola-and-rpos-genes-in-biofilm-formation-and-adherence-pattern-by-escherichia-coli-k-12-mg1655-on-polypropylene-stainless-steel-and-silicone-surfaces
#9
Mohd Adnan, Ana Margarida Sousa, Idalina Machado, Maria Olivia Pereira, Saif Khan, Glyn Morton, Sibte Hadi
Escherichia coli has developed sophisticated means to sense, respond, and adapt in stressed environment. It has served as a model organism for studies in molecular genetics and physiology since the 1960s. Stress response genes are induced whenever a cell needs to adapt and survive under unfavorable growth conditions. Two of the possible important genes are rpoS and bolA. The rpoS gene has been known as the alternative sigma (σ) factor, which controls the expression of a large number of genes, which are involved in responses to various stress factors as well as transition to stationary phase from exponential form of growth...
November 15, 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27834703/complete-genome-sequence-of-neb-5-alpha-a-derivative-of-escherichia-coli-k-12-dh5%C3%AE
#10
Brian P Anton, Elisabeth A Raleigh
Escherichia coli K-12 DH5α is one of the most popular and widely available laboratory strains, but, surprisingly, no complete genome sequence has been publicly available. Here, we report the complete, finished sequence of NEB 5-alpha (DH5α fhuA2). It should serve as a useful reference for researchers working with DH5α.
November 10, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27834244/government-spending-in-health-and-nonhealth-sectors-associated-with-improvement-in-county-health-rankings
#11
J Mac McCullough, Jonathon P Leider
There is little evidence to demonstrate the impact on local health outcomes of spending that occurs outside the health care sector. We leveraged novel data from the US Census Bureau to measure the independent impact of a community's health and nonhealth expenditures on a broad measure of overall health-the County Health Rankings-over time. Using lagged longitudinal models that accounted for correlations of health outcomes and expenditures within counties, we found significant positive associations between expenditures and County Health Rankings for seven of the fourteen expenditure categories examined: community health care and public health, public hospitals, fire protection, K-12 education, corrections, libraries, and housing and community development...
November 1, 2016: Health Affairs
https://www.readbyqxmd.com/read/27832064/project-bioeyes-accessible-student-driven-science-for-k-12-students-and-teachers
#12
Jamie R Shuda, Valerie G Butler, Robert Vary, Steven A Farber
BioEYES, a nonprofit outreach program using zebrafish to excite and educate K-12 students about science and how to think and act like scientists, has been integrated into hundreds of under-resourced schools since 2002. During the week-long experiments, students raise zebrafish embryos to learn principles of development and genetics. We have analyzed 19,463 participating students' pre- and post-tests within the program to examine their learning growth and attitude changes towards science. We found that at all grade levels, BioEYES effectively increased students' content knowledge and produced favorable shifts in students' attitudes about science...
November 2016: PLoS Biology
https://www.readbyqxmd.com/read/27821046/spontaneous-mutations-in-the-flhd-operon-generate-motility-heterogeneity-in-escherichia-coli-biofilm
#13
Shelley M Horne, Joseph Sayler, Nicholas Scarberry, Meredith Schroeder, Ty Lynnes, Birgit M Prüß
BACKGROUND: Heterogeneity and niche adaptation in bacterial biofilm involve changes to the genetic makeup of the bacteria and gene expression control. We hypothesized that i) spontaneous mutations in the flhD operon can either increase or decrease motility and that ii) the resulting motility heterogeneity in the biofilm might lead to a long-term increase in biofilm biomass. RESULTS: We allowed the highly motile E. coli K-12 strain MC1000 to form seven- and fourteen-day old biofilm, from which we recovered reduced motility isolates at a substantially greater frequency (5...
November 8, 2016: BMC Microbiology
https://www.readbyqxmd.com/read/27815466/fluorinated-beta-diketo-phosphorus-ylides-are-novel-efflux-pump-inhibitors-in-bacteria
#14
Annamária Kincses, Ágnes Míra Szabó, Ryosuke Saijo, Genki Watanabe, Masami Kawase, Joseph Molnár, Gabriella Spengler
BACKGROUND: One of the most important resistance mechanisms in bacteria is the increased expression of multidrug efflux pumps. To combat efflux-related resistance, the development of new efflux pump inhibitors is essential. MATERIALS AND METHODS: Ten phosphorus ylides were compared based on their MDR-reverting activity in multidrug efflux pump system consisting of the subunits acridine-resistance proteins A and B (AcrA and AcrB) and the multidrug efflux pump outer membrane factor TolC (TolC) of Escherichia coli K-12 AG100 strain and its AcrAB-TolC-deleted strain...
November 2016: In Vivo
https://www.readbyqxmd.com/read/27808174/essential-oils-and-eugenols-inhibit-biofilm-formation-and-the-virulence-of-escherichia-coli-o157-h7
#15
Yong-Guy Kim, Jin-Hyung Lee, Giyeon Gwon, Soon-Il Kim, Jae Gyu Park, Jintae Lee
Enterohemorrhagic Escherichia coli O157:H7 (EHEC) has caused foodborne outbreaks worldwide and the bacterium forms antimicrobial-tolerant biofilms. We investigated the abilities of various plant essential oils and their components to inhibit biofilm formation by EHEC. Bay, clove, pimento berry oils and their major common constituent eugenol at 0.005% (v/v) were found to markedly inhibit EHEC biofilm formation without affecting planktonic cell growth. In addition, three other eugenol derivatives isoeugenol, 2-methoxy-4-propylphenol, and 4-ethylguaiacol had antibiofilm activity, indicating that the C-1 hydroxyl unit, the C-2 methoxy unit, and C-4 alkyl or alkane chain on the benzene ring of eugenol play important roles in antibiofilm activity...
November 3, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27794218/community-integration-of-people-living-with-epilepsy-in-a-nigerian-population
#16
Emmanuel Chiebuka Okoye, Grace Chinyere Obi, Christopher Olusanjo Akosile, Joseph Onuwa Umunnah, Maduabuchi Joseph Nwankwo, Loveth Oluchi Obiora
BACKGROUND: Epilepsy is a chronic seizure disorder that affects numerous people worldwide. Community integration (CI) is the ultimate goal of rehabilitation of any chronic condition. There seems to be a dearth of research on CI among people living with epilepsy (PLWE). AIM: The present study was designed to investigate on the level of satisfaction with CI and its associated factors in a Nigerian PLWE. METHODOLOGY: This was a cross-sectional survey of 70 adult PLWE (28...
December 2016: Epilepsy Research
https://www.readbyqxmd.com/read/27793830/benzoate-and-salicylate-tolerant-strains-lose-antibiotic-resistance-during-laboratory-evolution-of-escherichia-coli-k-12
#17
Kaitlin E Creamer, Frederick S Ditmars, Preston J Basting, Karina S Kunka, Issam N Hamdallah, Sean P Bush, Zachary Scott, Amanda He, Stephanie R Penix, Alexandra S Gonzales, Elizabeth K Eder, Dominic Camperchioli, Adama Berndt, Michelle W Clark, Kerry A Rouhier, Joan L Slonczewski
: Escherichia coli K-12 W3110 grows in the presence of membrane-permeant organic acids that can depress cytoplasmic pH and accumulate in the cytoplasm. We conducted experimental evolution by daily diluting cultures in increasing concentrations of benzoic acid (up to 20 mM) buffered at external pH 6.5, a pH at which permeant acids concentrate in the cytoplasm. By 2,000 generations, clones isolated from evolving populations showed increasing tolerance to benzoate but were sensitive to chloramphenicol and tetracycline...
October 28, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27780377/parent-perceptions-of-their-child-s-communication-and-academic-experiences-with-cochlear-implants
#18
Lauri H Nelson, Lindsey Herde, Karen Munoz, Karl R White, Michael D Page
OBJECTIVE: To explore parent perceptions of communication and academic experiences of cochlear implant (CI) recipients under the age of 18. DESIGN: Cross-sectional survey design. Two surveys (K-12, birth-5) queried parent perceptions of the impact of CI on communication, listening in various environments and academic achievement. Surveys contained 16-23 multiple choice and/or Likert ratings, plus four open-ended questions to assess reasons for obtaining a CI, satisfaction with CI and suggestions for professionals...
October 26, 2016: International Journal of Audiology
https://www.readbyqxmd.com/read/27753484/efficient-nanofibrous-membranes-for-antibacterial-wound-dressing-and-uv-protection
#19
Shuai Jiang, Beatriz Chiyin Ma, Jonas Reinholz, Qifeng Li, Junwei Wang, Kai A I Zhang, Katharina Landfester, Daniel Crespy
Materials with a hierarchical structure often demonstrate superior properties with combined and even synergistic effects of multiple functions. Herein, we report the design of a new class of material with a multicompartment nanofibrous structure as a promising candidate for antibacterial wound dressing and functional textile applications. The design consists in first synthesizing nanocapsules loaded with functional payloads and subsequently embedding the nanocapsules into polymer nanofibers by using colloid-electrospinning technique...
October 18, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27743797/enhanced-plasmid-loss-in-bacterial-populations-exposed-to-the-antimicrobial-compound-irgasan-delivered-from-interpenetrating-polymer-network-silicone-hydrogels
#20
Leise Riber, Mette Burmølle, Martin Alm, Stefan M Milani, Peter Thomsen, Lars H Hansen, Søren J Sørensen
The spread of antimicrobial resistance, usually mediated by horizontal transfer of plasmids, limits the options of treating bacterial infections and thereby poses a crucial human health problem. The disturbance of plasmid stability within bacterial species in clinical environments serves as a novel strategy to reduce the development and dissemination of antibiotic resistance. We tested the ability of irgasan to destabilize plasmids from Escherichia coli K-12 cells when added directly into liquid growth medium at concentrations below levels of marked bacterial growth inhibition, or when released into liquid growth medium from irgasan-impregnated Interpenetrating Polymer Network (IPN) silicone hydrogel objects, a novel technology developed as drug-delivery platform...
October 12, 2016: Plasmid
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