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https://www.readbyqxmd.com/read/29040409/towards-dynamic-genome-scale-models
#1
David Gilbert, Monika Heiner, Yasoda Jayaweera, Christian Rohr
The analysis of the dynamic behaviour of genome-scale models of metabolism (GEMs) currently presents considerable challenges because of the difficulties of simulating such large and complex networks. Bacterial GEMs can comprise about 5000 reactions and metabolites, and encode a huge variety of growth conditions; such models cannot be used without sophisticated tool support. This article is intended to aid modellers, both specialist and non-specialist in computerized methods, to identify and apply a suitable combination of tools for the dynamic behaviour analysis of large-scale metabolic designs...
October 13, 2017: Briefings in Bioinformatics
https://www.readbyqxmd.com/read/29032558/recombinant-e-coli-dualistic-role-as-an-antigen-adjuvant-delivery-vehicle-for-oral-immunization
#2
Maria Gomes-Solecki, Luciana Richer
Escherichia coli is the mainstay tool for fundamental microbiology research due to its ease of cultivation and safety. Auxotrophic strains of the K-12 and B lineages of E. coli are the organisms of choice to produce recombinant proteins. Components present in the cell envelope of bacteria are also potent immune modulators and have been used to develop adjuvants. We used live E. coli, after induction of recombinant protein expression, to develop a vehicle which has a dualistic function of producing vaccine while presenting itself as the adjuvant to deliver oral vaccines against a number of infectious diseases, including Lyme disease...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28994355/treating-depression-with-physical-activity-in-adolescents-and-young-adults-a-systematic-review-and-meta-analysis-of-randomised-controlled-trials
#3
A P Bailey, S E Hetrick, S Rosenbaum, R Purcell, A G Parker
We aimed to establish the treatment effect of physical activity for depression in young people through meta-analysis. Four databases were searched to September 2016 for randomised controlled trials of physical activity interventions for adolescents and young adults, 12-25 years, experiencing a diagnosis or threshold symptoms of depression. Random-effects meta-analysis was used to estimate the standardised mean difference (SMD) between physical activity and control conditions. Subgroup analysis and meta-regression investigated potential treatment effect modifiers...
October 10, 2017: Psychological Medicine
https://www.readbyqxmd.com/read/28986371/genotypic-and-phenotypic-characteristics-in-association-with-biofilm-formation-in-different-pathotypes-of-human-clinical-escherichia-coli-isolates
#4
Juliane Schiebel, Alexander Böhm, Jörg Nitschke, Michał Burdukiewicz, Jörg Weinreich, Aamir Ali, Dirk Roggenbuck, Stefan Rödiger, Peter Schierack
Bacterial biofilm formation is a widespread phenomenon and a complex process requiring a set of genes facilitating the initial adhesion, maturation, production of extracellular polymeric matrix (EPS) and subsequent dispersal of bacteria. Most studies on Escherichia coli (E. coli) biofilm formation have investigated non-pathogenic E. coli K-12 strains. Due to the extensive focus on laboratory strains in most studies, there is poor information regarding biofilm formation by pathogenic E. coli isolates. In this study, we genotypically and phenotypically characterized 187 human clinical E...
October 6, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28983282/binding-of-escherichia-coli-does-not-protect-tulane-virus-from-heat-inactivation-regardless-the-expression-of-hbga-like-molecules
#5
Qianqian Li, Dapeng Wang, David Yang, Lei Shan, Peng Tian
Histo-blood group antigens (HBGAs) are considered as receptors/co-receptors for human norovirus (HuNoV). It has been reported that binding of HuNoV-derived virus-like particles (VLPs) to HBGA-like molecules-expressing bacteria increased the stability of VLPs to heat-denaturation (HD). In this study, we tested for HBGA-like-binding-conveyed protection against HD on viral replication using Tulane virus (TV) and Escherichia coli O86:H2 (O86:H2), with E. coli K-12 (K-12) used as a control. Expression of HBGA type B was confirmed by ELISA in O86:H2 but not in K-12...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28982238/carbon-kinetic-isotope-effects-and-the-mechanisms-of-acid-catalyzed-decarboxylation-of-2-4-dimethoxybenzoic-acid-and-co2-incorporation-into-1-3-dimethoxybenzene
#6
Adelle A Vandersteen, Graeme W Howe, Barbara Sherwood Lollar, Ronald Kluger
The rate of decarboxylation of 2,4-dimethoxybenzoic acid (1) is accelerated in parallel to the extent that the carboxyl group acquires a second proton (1H(+)). However, the conjugate acid would resist C-C bond breaking as that would lead to formation of doubly protonated CO2. An alternative via formation of a higher-energy protonated phenyl tautomer (2H(+)) prior to C-C bond breaking would produce protonated CO2, an energetically inaccessible species that can be avoided by transfer of the carboxyl proton to water in the same step...
October 13, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28964852/the-effectiveness-of-school-based-physical-activity-interventions-for-adolescent-girls-a-systematic-review-and-meta-analysis
#7
REVIEW
Michael B Owen, Whitney B Curry, Charlotte Kerner, Lisa Newson, Stuart J Fairclough
Physical activity (PA) decreases during the transition from childhood to adolescence, with larger declines observed in girls. School-based interventions are considered the most promising approach for increasing adolescents' PA levels although, it is unclear which types of school-based interventions have the greatest impact. The objective of this systematic review is to assess the impact and design of school-based PA interventions targeting adolescent girls. A systematic search was conducted using four electronic databases (PubMed, Web of Science, SPORTDiscus and PsychInfo)...
September 28, 2017: Preventive Medicine
https://www.readbyqxmd.com/read/28961148/developing-an-engineering-design-process-assessment-using-mixed-methods
#8
Stefanie A Wind, Meltem Alemdar, Jeremy A Lingle, Jessica D Gale, Roxanne A Moore
Recent reforms in science education worldwide include an emphasis on engineering design as a key component of student proficiency in the Science, Technology, Engineering, and Mathematics disciplines. However, relatively little attention has been directed to the development of psychometrically sound assessments for engineering. This study demonstrates the use of mixed methods to guide the development and revision of K-12 Engineering Design Process (EDP) assessment items. Using results from a middle-school EDP assessment, this study illustrates the combination of quantitative and qualitative techniques to inform item development and revisions...
2017: Journal of Applied Measurement
https://www.readbyqxmd.com/read/28959742/the-biofilm-inhibitor-carolacton-enters-gram-negative-cells-studies-using-a-tolc-deficient-strain-of-escherichia-coli
#9
Jannik Donner, Michael Reck, Boyke Bunk, Michael Jarek, Constantin Benjamin App, Jan P Meier-Kolthoff, Jörg Overmann, Rolf Müller, Andreas Kirschning, Irene Wagner-Döbler
The myxobacterial secondary metabolite carolacton inhibits growth of Streptococcus pneumoniae and kills biofilm cells of the caries- and endocarditis-associated pathogen Streptococcus mutans at nanomolar concentrations. Here, we studied the response to carolacton of an Escherichia coli strain that lacked the outer membrane protein TolC. Whole-genome sequencing of the laboratory E. coli strain TolC revealed the integration of an insertion element, IS5, at the tolC locus and a close phylogenetic relationship to the ancient E...
September 2017: MSphere
https://www.readbyqxmd.com/read/28951266/fast-growth-phenotype-of-e-coli-k-12-from-adaptive-laboratory-evolution-does-not-require-intracellular-flux-rewiring
#10
Christopher P Long, Jacqueline E Gonzalez, Adam M Feist, Bernhard O Palsson, Maciek R Antoniewicz
Adaptive laboratory evolution (ALE) is a widely-used method for improving the fitness of microorganisms in selected environmental conditions. It has been applied previously to Escherichia coli K-12 MG1655 during aerobic exponential growth on glucose minimal media, a frequently used model organism and growth condition, to probe the limits of E. coli growth rate and gain insights into fast growth phenotypes. Previous studies have described up to 1.6-fold increases in growth rate following ALE, and have identified key causal genetic mutations and changes in transcriptional patterns...
September 23, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28945444/helping-america-s-most-vulnerable-children-and-parents
#11
Michael S Wald
This article focuses on ways the government should allocate resources in order to support America's most vulnerable children and parents. Government policies on helping children seek to achieve many outcomes, but four are especially important: (a) protecting children's basic physical safety and mental safety (i.e., sense of emotional security in relationships with one's parents) during childhood; (b) ensuring that children do not suffer from serious mental health problems that impair their adjustment and ability to function well at home, at school, and in social relations; (c) helping all children acquire the academic skills needed for economic "success" (i...
2017: American Journal of Orthopsychiatry
https://www.readbyqxmd.com/read/28938191/blood-lead-and-preeclampsia-a-meta-analysis-and-review-of-implications
#12
Arthur E Poropat, Mark A S Laidlaw, Bruce Lanphear, Andrew Ball, Howard W Mielke
BACKGROUND: Multiple cross-sectional studies suggest that there is an association between blood lead and preeclampsia. OBJECTIVES: We performed a systematic review and meta-analysis to summarize information on the association between preeclampsia and lead poisoning. METHODS: Searches of Medline, Web of Science, Scopus, Pubmed, Science Direct and ProQuest (dissertations and theses) identified 2089 reports, 46 of which were downloaded after reviewing the abstracts, and 11 studies were evaluated as meeting the selection criteria...
September 19, 2017: Environmental Research
https://www.readbyqxmd.com/read/28927723/antimutagenic-activity-of-vitamin-b1-against-damages-induced-by-chemical-and-physical-mutagens-in-salmonella-typhimurium-and-escherichia-coli
#13
Jaime Sánchez-Navarrete, Myriam Arriaga-Alba, Nancy Jannet Ruiz-Pérez, Julia Dolores Toscano-Garibay
Thiamine (vitamin B1) is an essential nutrient acting mainly as an enzymatic cofactor on diverse cell processes. It has been reported that vitamin B1 has a significant role in the signaling pathways related to the response to adverse environmental conditions (chemical and physical). The objectives of this study were to evaluate the antimutagenic potential of vitamin B1 in front of DNA-alkylating agents in the presence/absence of ogt and ada repairing genes in Salmonella typhimurium strains and against damage induced by ultraviolet light type C in Escherichia coli strains mutated at the uvrABC system and recBCD enzymes...
December 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28925955/investing-in-the-early-childhood-mental-health-workforce-development-enhancing-professionals-competencies-to-support-emotion-and-behavior-regulation-in-young-children
#14
Shulamit N Ritblatt, Audrey Hokoda, Charles Van Liew
This paper delineates a preventive approach to early childhood mental health by preparing the workforce to provide relational, sensitive care to young children ages 0-5. One of the most prevalent issues in early childhood is behavioral challenges and the inability of young children to regulate themselves. This leads to an expulsion rate in early childhood (3-4 times higher than K-12 expulsion rate) and future mental health issues. The Early Childhood Social-Emotional and Behavior Regulation Intervention Specialist (EC-SEBRIS) graduate level certificate program was created to strengthen early care and education providers with the knowledge and practice of how to support emotion and behavior regulation in young children in their groups...
September 19, 2017: Brain Sciences
https://www.readbyqxmd.com/read/28915089/transgender-teachers-the-personal-pedagogical-and-political
#15
Kristopher Wells
This empirical research study examines the experiences of three male-to-female transgender teachers who transitioned genders, in three different decades (1980s, 1990s, 2000s) while actively teaching within Canadian K-12 public schools. I utilize poststructural storylines to explore how these transgender teachers navigated the personal, pedagogical, and political and the survival and transition strategies they developed to become intelligible within their schools. Their storylines illustrate how they developed counternarratives to challenge traditional discourses of trans invisibility, silence, shame, and fear...
September 15, 2017: Journal of Homosexuality
https://www.readbyqxmd.com/read/28906353/immunomodulatory-treatments-for-persistent-and-chronic-immune-thrombocytopenic-purpura-a-prisma-compliant-systematic-review-and-meta-analysis-of-28-studies
#16
REVIEW
Emmanuelle Weber, Quitterie Reynaud, Romain Fort, Stéphane Durupt, Pascal Cathébras, Isabelle Durieu, Jean-Christophe Lega
BACKGROUND: Corticosteroid sparing is required in 15% to 40% of adults with persistent or chronic primary immune thrombocytopenic purpura (ITP). Herein, the efficacy of immunomodulatory drugs (dapsone, interferon alpha, danazol, and hydroxychloroquine as second-third-line therapies in ITP is investigated. METHODS: MEDLINE was searched for studies that included patients with persistent or chronic primary ITP and published before the end of December 2014. Two investigators independently extracted data regarding study design, patient characteristics, dosage schedule, time to response, and occurrence of adverse events...
September 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28904250/building-a-complete-image-of-genome-regulation-in-the-model-organism-escherichia-coli
#17
Akira Ishihama
The model organism, Escherichia coli, contains a total of more than 4,500 genes, but the total number of RNA polymerase (RNAP) core enzyme or the transcriptase is only about 2,000 molecules per genome. The regulatory targets of RNAP are, however, modulated by changing its promoter selectivity through two-steps of protein-protein interplay with 7 species of the sigma factor in the first step, and then 300 species of the transcription factor (TF) in the second step. Scientists working in the field of prokaryotic transcription in Japan have made considerable contributions to the elucidation of genetic frameworks and regulatory modes of the genome transcription in E...
September 12, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28876228/hicab-toxin-antitoxin-complex-from-escherichia-coli-expression-and-crystallization
#18
Jingsi Yang, Bingshuang Xu, Zengqiang Gao, Ke Zhou, Peng Liu, Yuhui Dong, Jianjun Zhang, Quansheng Liu
Toxin-antitoxin (TA) systems are widespread in both bacteria and archaea, where they enable cells to adapt to environmental cues. TA systems play crucial roles in various cellular processes, such as programmed cell death, cell growth, persistence and virulence. Here, two distinct forms of the type II toxin-antitoxin complex HicAB were identified and characterized in Escherichia coli K-12, and both were successfully overexpressed and purified. The two proposed forms, HicAB(L) and HicAB(S), differed in the presence or absence of a seven-amino-acid segment at the N-terminus in the antitoxin HicB...
September 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28875432/a-meta-analytic-review-of-the-association-between-cortisol-reactivity-in-response-to-a-stressor-and-attention-deficit-hyperactivity-disorder
#19
Jaclyn M Kamradt, Allison M Momany, Molly A Nikolas
A substantial literature suggests that abnormal cortisol reactivity may be a vulnerability for deleterious mental health outcomes, including ADHD. ADHD has been linked with difficulty in emotion regulation and increased risk of experiencing stressors, both of which may be related to psychobiological abnormalities (e.g., abnormal cortisol reactivity). Research has been mixed regarding the association between cortisol reactivity and ADHD. Therefore, the present meta-analytic review (k = 12) sought to quantify this association and review the relevant methodological issues and theoretical implications of this area of research...
September 5, 2017: Attention Deficit and Hyperactivity Disorders
https://www.readbyqxmd.com/read/28870009/comprehensive-analysis-of-proteomic-differences-between-escherichia-coli-k-12-and-b-strains-using-multiplexed-isobaric-tandem-mass-tag-tmt-labeling
#20
Mee-Jung Han
The Escherichia coli K-12 and B strains are among the most frequently used bacterial hosts for scientific research and biotechnological applications. However, omics analyses have revealed that E. coli K-12 and B exhibit notably different genotypic and phenotypic attributes, even though they were derived from the same ancestor. In a previous study, we identified a limited number of proteins from the two strains using two-dimensional gel electrophoresis and tandem mass spectrometry (MS/MS). In this study, an in-depth analysis of the physiological behavior of the E...
September 5, 2017: Journal of Microbiology and Biotechnology
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