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https://www.readbyqxmd.com/read/29313097/efficient-production-of-lycopene-by-engineered-e-coli-strains-harboring-different-types-of-plasmids
#1
Jiali Xu, Xian Xu, Qing Xu, Zhidong Zhang, Ling Jiang, He Huang
The lycopene biosynthetic genes crtE, crtB, and crtI from Deinococcus wulumuqiensis R12 were integrated into three different vector backbones-pET28a, pTrc99A, and pUC18-and the resulting recombinant plasmids pET28a-EBI, pTrc99A-EBI, and pUC18-EBI were introduced into different Escherichia coli hosts. The results showed that lycopene production of strain 28BL was lower than that of 99 series strains without IPTG in LB medium. In addition, lycopene production of 99JM with supplementation of 20% (w/v) glycerol was 1...
January 8, 2018: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/29276501/host-and-pathway-engineering-for-enhanced-lycopene-biosynthesis-in-yarrowia-lipolytica
#2
Cory Schwartz, Keith Frogue, Joshua Misa, Ian Wheeldon
Carotenoids are a class of molecules with commercial value as food and feed additives with nutraceutical properties. Shifting carotenoid synthesis from petrochemical-based precursors to bioproduction from sugars and other biorenewable carbon sources promises to improve process sustainability and economics. In this work, we engineered the oleaginous yeast Yarrowia lipolytica to produce the carotenoid lycopene. To enhance lycopene production, we tested a series of strategies to modify host cell physiology and metabolism, the most successful of which were mevalonate pathway overexpression and alleviating auxotrophies previously engineered into the PO1f strain of Y...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29202518/-construction-of-high-yield-strain-by-optimizing-lycopene-cyclase-for-%C3%AE-carotene-production
#3
Yingfu Jin, Li Han, Shasha Zhang, Shizhong Li, Weifeng Liu, Yong Tao
To optimize key enzymes, such as to explore the gene resources and to modify the expression level, can maximize metabolic pathways of target products. β-carotene is a terpenoid compound with important application value. Lycopene cyclase (CrtY) is the key enzyme in β-carotene biosynthesis pathway, catalyzing flavin adenine dinucleotide (FAD)-dependent cyclization reaction and β-carotene synthesis from lycopene precursor. We optimized lycopene cyclase (CrtY) to improve the synthesis of β-carotene and determined the effect of CrtY expression on metabolic pathways...
November 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29191130/structural-and-functional-analysis-of-the-carotenoid-biosynthesis-genes-of-a-pseudomonas-strain-isolated-from-the-excrement-of-autumn-darter
#4
Yuki Fukaya, Miho Takemura, Takashi Koyanagi, Takashi Maoka, Kazutoshi Shindo, Norihiko Misawa
There are many reports about carotenoid-producing bacteria and carotenoid biosynthesis genes. In databases for Pseudomonas genome sequences, there are genes homologous to carotenoid biosynthesis genes, but the function of these genes in Pseudomonas has not been elucidated. In this study, we cloned the carotenoid biosynthesis genes from a Pseudomonas sp. strain, named Akiakane, which was isolated from the excrement of the Autumn Darter dragonfly. Using an Escherichia coli functional expression system, we confirmed that the idi, crtE, crtB, crtI, and crtY gene products of the Akiakane strain show predictable catalytic activities...
December 1, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28904416/knockout-of-pprm-decreases-resistance-to-desiccation-and-oxidation-in-deinococcus-radiodurans
#5
Yang Zeng, Yun Ma, Fangzhu Xiao, Wuzhou Wang, Shuya He
Deinococcus radiodurans has attracted a great interest in the past decades due to its extraordinary resistance to ionizing radiation and highly efficient DNA repair system. Recent studies indicated that pprM is a putative pleiotropic gene in D. radiodurans and plays an important role in radioresistance and antioxidation, but its underlying mechanisms are poorly elucidated. In this study, pprM mutation was generated to investigate resistance to desiccation and oxidative stress. The result showed that the survival of pprM mutant under desiccation was markedly retarded compared to the wild strain from day 7-28...
September 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/28761150/single-genetic-locus-improvement-of-iron-zinc-and-%C3%AE-carotene-content-in-rice-grains
#6
Simrat Pal Singh, Wilhelm Gruissem, Navreet K Bhullar
Nearly half of the world's population obtains its daily calories from rice grains, which lack or have insufficient levels of essential micronutrients. The deficiency of micronutrients vital for normal growth is a global health problem, and iron, zinc and vitamin A deficiencies are the most prevalent ones. We developed rice lines expressing Arabidopsis NICOTIANAMINE SYNTHASE 1 (AtNAS1), bean FERRITIN (PvFERRITIN), bacterial CAROTENE DESATURASE (CRTI) and maize PHYTOENE SYNTHASE (ZmPSY) in a single genetic locus in order to increase iron, zinc and β-carotene content in the rice endosperm...
July 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28669634/structure-of-phytoene-desaturase-provides-insights-into-herbicide-binding-and-reaction-mechanisms-involved-in-carotene-desaturation
#7
Anton Brausemann, Sandra Gemmecker, Julian Koschmieder, Sandro Ghisla, Peter Beyer, Oliver Einsle
Cyanobacteria and plants synthesize carotenoids via a poly-cis pathway starting with phytoene, a membrane-bound C40 hydrocarbon. Phytoene desaturase (PDS) introduces two double bonds and concomitantly isomerizes two neighboring double bonds from trans to cis. PDS assembles into homo-tetramers that interact monotopically with membranes. A long hydrophobic tunnel is proposed to function in the sequential binding of phytoene and the electron acceptor plastoquinone. The herbicidal inhibitor norflurazon binds at a plastoquinone site thereby blocking reoxidation of FADred...
August 1, 2017: Structure
https://www.readbyqxmd.com/read/28371918/functional-lycopene-cyclase-crua-in-cyanobacterium-arthrospira-platensis-nies-39-and-its-role-in-carotenoid-synthesis
#8
Kenjiro Sugiyama, Masashi Ebisawa, Masaharu Yamada, Yoshiki Nagashima, Hideyuki Suzuki, Takashi Maoka, Shinichi Takaichi
The genus Arthrospira is filamentous, non-nitrogen-fixing cyanobacteria that is commercially important. We identified the molecular structures of carotenoids in Arthrospira platensis NIES-39. The major carotenoid identified was β-carotene. In addition, the hydroxyl derivatives of β-cryptoxanthin and (3R,3'R)-zeaxanthin were also found to be present. The carotenoid glycosides were identified as (3R,2'S)-myxol 2'-methylpentoside and oscillol 2,2'-dimethylpentoside. The methylpentoside moiety was a mixture of fucoside and chinovoside in an approximate ratio of 1 : 4...
April 1, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28337556/homologous-gene-targeting-of-a-carotenoids-biosynthetic-gene-in-rhodosporidium-toruloides-by-agrobacterium-mediated-transformation
#9
Wenyi Sun, Xiaobing Yang, Xueying Wang, Xinping Lin, Yanan Wang, Sufang Zhang, Yushi Luan, Zongbao K Zhao
OBJECTIVES: To target a carotenoid biosynthetic gene in the oleaginous yeast Rhodosporidium toruloides by using the Agrobacterium-mediated transformation (AMT) method. RESULTS: The RHTO_04602 locus of R. toruloides NP11, previously assigned to code the carotenoid biosynthetic gene CRTI, was amplified from genomic DNA and cloned into the binary plasmid pZPK-mcs, resulting in pZPK-CRT. A HYG-expression cassette was inserted into the CRTI sequence of pZPK-CRT by utilizing the restriction-free clone strategy...
March 23, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28284309/alleviation-of-metabolic-bottleneck-by-combinatorial-engineering-enhanced-astaxanthin-synthesis-in-saccharomyces-cerevisiae
#10
Pingping Zhou, Wenping Xie, Aipeng Li, Fan Wang, Zhen Yao, Qi Bian, Yongqiang Zhu, Hongwei Yu, Lidan Ye
Highly efficient biosynthesis of the commercially valuable carotenoid astaxanthin by microbial cells is an attractive alternative to chemical synthesis and microalgae extraction. With the goal of enhancing heterologous astaxanthin production in Saccharomyces cerevisiae, metabolic engineering and protein engineering were integrated to improve both the expression and activity of rate-limiting enzymes. Firstly, to increase the supply of β-carotene as a key precursor for astaxanthin, a positive mutant of GGPP synthase (CrtE03M) was overexpressed together with three other rate-limiting enzymes tHMG1, CrtI and CrtYB...
May 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28263769/development-of-xanthophyllomyces-dendrorhous-as-a-production-system-for-the-colorless-carotene-phytoene
#11
Hendrik Pollmann, Jürgen Breitenbach, Gerhard Sandmann
Phytoene is a colorless carotenoid with increasing economic potential for skin care but with limited availability. The red yeast Xanthophyllomyces dendrorhous which has previously been used as a production platform for carotenoids was engineered as a prototype for the yield of this carotene. Phytoene was accumulated by prevention of its metabolization by desaturation in the carotenoid pathway. In a first step, the phytoene desaturase gene crtI was disrupted by insertion of a hygromycin-resistance gene. Most of the resulting transformants were heterozygote for intact and inactivated crtI...
April 10, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28094982/bacterial-genome-editing-via-a-designed-toxin-antitoxin-cassette
#12
Jie Wu, Aihua Deng, Qinyun Sun, Hua Bai, Zhaopeng Sun, Xiuling Shang, Yun Zhang, Qian Liu, Yong Liang, Shuwen Liu, Yongsheng Che, Tingyi Wen
Manipulating the bacterial genomes in an efficient manner is essential to biological and biotechnological research. Here, we reprogrammed the bacterial TA systems as the toxin counter-selectable cassette regulated by an antitoxin switch (TCCRAS) for genetic modifications in the extensively studied and utilized Gram-positive bacteria, B. subtilis and Corynebacterium glutamicum. In the five characterized type II TA systems, the RelBE complex can specifically and efficiently regulate cell growth and death by the conditionally controlled antitoxin RelB switch, thereby serving as a novel counter-selectable cassette to establish the TCCRAS system...
January 26, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28068433/molecular-and-functional-characterization-of-gr2-r1-event-based-backcross-derived-lines-of-golden-rice-in-the-genetic-background-of-a-mega-rice-variety-swarna
#13
Haritha Bollinedi, Gopala Krishnan S, Kumble Vinod Prabhu, Nagendra Kumar Singh, Sushma Mishra, Jitendra P Khurana, Ashok Kumar Singh
Homozygous Golden Rice lines developed in the background of Swarna through marker assisted backcross breeding (MABB) using transgenic GR2-R1 event as a donor for the provitamin A trait have high levels of provitamin A (up to 20 ppm) but are dwarf with pale green leaves and drastically reduced panicle size, grain number and yield as compared to the recurrent parent, Swarna. In this study, we carried out detailed morphological, biochemical and molecular characterization of these lines in a quest to identify the probable reasons for their abnormal phenotype...
2017: PloS One
https://www.readbyqxmd.com/read/27865019/the-regulation-of-carotenoid-formation-in-tomato-fruit
#14
Eugenia M A Enfissi, Marilise Nogueira, Peter M Bramley, Paul D Fraser
Carotenoid biosynthesis in plants includes a complex series of desaturation/isomerisation reactions, catalyzed by four independent enzymes. In bacteria and fungi one desaturase/isomerase enzyme completes the same series of reactions. In the present study, a bacterial desaturase (crtI) from Pantoea ananatis has been overexpressed in the tangerine mutant of tomato (Solanum lycopersicon) which accumulates cis-carotene isomers in the fruit due to a defective isomerase (CRTISO) and the old gold crimson (og(c) ) tomato mutant, which is defective in the fruit-enhanced lycopene β-cyclase (CYCB)...
November 19, 2016: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/27677495/expression-of-lycopene-biosynthesis-genes-fused-in-line-with-shine-dalgarno-sequences-improves-the-stress-tolerance-of-lactococcus-lactis
#15
Xiangrong Dong, Yanping Wang, Fengyuan Yang, Shanshan Zhao, Bing Tian, Tao Li
OBJECTIVES: Lycopene biosynthetic genes from Deinococcus radiodurans were co-expressed in Lactococcus lactis to produce lycopene and improve its tolerance to stress. RESULTS: Lycopene-related genes from D. radiodurans, DR1395 (crtE), DR0862 (crtB), and DR0861 (crtI), were fused in line with S hine-Dalgarno (SD) sequences and co-expressed in L. lactis. The recombinant strain produced 0.36 mg lycopene g(-1) dry cell wt after 48 h fermentation. The survival rate to UV irradiation of the recombinant strain was higher than that of the non-transformed strain...
January 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/27564699/carotenoid-analysis-of-sweetpotato-ipomoea-batatas-and-functional-identification-of-its-lycopene-%C3%AE-and-%C3%AE%C2%B5-cyclase-genes
#16
Muhammad Zubair Khan, Miho Takemura, Takashi Maoka, Motoyasu Otani, Norihiko Misawa
Sweetpotato Ipomoea batatas is known as a hexaploid species. Here, we analyzed carotenoids contained in the leaves and tubers of sweetpotato cultivars 'White Star' (WS) and W71. These cultivars were found to contain several carotenoids unique to sweetpotato tubers such as β-carotene-5,6,5',8'-diepoxide and β-carotene-5,8-epoxide. Next, we isolated two kinds of carotene cyclase genes that encode lycopene β- and ε-cyclases from the WS and W71 leaves, by RT-PCR and subsequent RACE. Two and three lycopene β-cyclase gene sequences were, respectively, isolated from WS, named IbLCYb1, 2, and from W71, IbLCYb3, 4, 5...
September 1, 2016: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/27512519/draft-genome-sequence-of-carotenoid-producing-yellow-pigmented-planococcus-maritimus-mku009
#17
Ashok Ganapathy, Sridhar Jayavel, Sivakumar Natesan
Planococcus maritimus MKU009 is a Gram positive cocci and a moderate halophilic bacterium isolated from marine water of Pichavaram, South East Coast of India. Here we report the draft genome of Planococcus maritimus MKU009 with a total size of 3,251,644 bp with N50 value of 1681571 bp. The overall G+C content of the genome was 47.27%. The carotenoid producing crtN, crtB, crtP and crtI genes were located within the first contig of the genome assembly. This genome source will provide insights into functional genomics of carotenoid production and metabolic engineering...
2016: Journal of Genomics
https://www.readbyqxmd.com/read/27387846/pediatric-seizure-related-posttraumatic-stress-and-anxiety-symptoms-treated-with-emdr-a-case-series
#18
Elmedina Dautovic, Carlijn de Roos, Yanda van Rood, Agnes Dommerholt, Roos Rodenburg
PURPOSE: To examine the potential effects of eye movement desensitization and reprocessing (EMDR) in children with epilepsy-related posttraumatic stress and/or anxiety symptoms, using a case series design. METHODS: Five children (aged 8-18) with epilepsy identified for seizure-related posttraumatic stress and/or anxiety symptoms were treated with EMDR. To examine potential treatment effects, posttraumatic stress and anxiety symptoms were assessed (CRTI and SCARED) pre- and post-EMDR and at 3-month follow-up...
2016: European Journal of Psychotraumatology
https://www.readbyqxmd.com/read/27376307/production-of-the-marine-carotenoid-astaxanthin-by-metabolically-engineered-corynebacterium-glutamicum
#19
Nadja A Henke, Sabine A E Heider, Petra Peters-Wendisch, Volker F Wendisch
Astaxanthin, a red C40 carotenoid, is one of the most abundant marine carotenoids. It is currently used as a food and feed additive in a hundred-ton scale and is furthermore an attractive component for pharmaceutical and cosmetic applications with antioxidant activities. Corynebacterium glutamicum, which naturally synthesizes the yellow C50 carotenoid decaprenoxanthin, is an industrially relevant microorganism used in the million-ton amino acid production. In this work, engineering of a genome-reduced C. glutamicum with optimized precursor supply for astaxanthin production is described...
June 30, 2016: Marine Drugs
https://www.readbyqxmd.com/read/27346167/a-single-desaturase-gene-from-red-yeast-sporidiobolus-pararoseus-is-responsible-for-both-four-and-five-step-dehydrogenation-of-phytoene
#20
Chunji Li, Ning Zhang, Jia Song, Na Wei, Bingxue Li, Hongtao Zou, Xiaori Han
Carotenoids are one of the most common classes of natural pigments widely occurring within organisms. These structurally diverse pigments are of great importance in different processes such as nutrition, vision, cellular growth and development. While found in various yeast strains, one of the best-studied carotenoid producer is the pigmented species Sporidiobolus pararoseus. However, the precise nature of the genes involved in the biosynthesis of carotenoids in this species remains unclear. Here, we cloned a cDNA copy of the phytoene desaturase gene crtI from Sporidiobolus pararoseus CGMCC 2...
September 15, 2016: Gene
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