Yu-Ling Hung, Syuan-Fei Hong, Wei-Lun Wei, Shiuan Cheng, Jia-Zhen Yu, Veny Tjita, Qian-Yuan Yong, Ryuichi Nishihama, Takayuki Kohchi, John Bowman, Yuan-Chi Chien, Yen-Hsin Chiu, Ho-Chun Yang, Mei-Yeh Jade Lu, Zhao-Jun Pan, Chun-Neng Wang, Shih-Shun Lin
The miR390-derived TAS3 trans-acting short-interfering RNAs (tasiRNAs) module represents a conserved RNA silencing pathway in the plant kingdom; however, its characterization in the bryophyte Marchantia polymorpha is limited. This study elucidated that MpDCL4 processes MpTAS3 double-stranded RNA (dsRNA) to generate tasiRNAs, primarily from the 5'- and 3'-ends of dsRNA. Notably, we discovered a novel tasiRNA, tasi78A, can negatively regulate a cytochrome P450 gene, MpCYP78A101. Additionally, tasi78A was abundant in MpAGO1, and transient expression assays underscored the role of tasi78A in repressing MpCYP78A101...
March 28, 2024: Plant & Cell Physiology