keyword
MENU ▼
Read by QxMD icon Read
search

Bone Demineralization

keyword
https://www.readbyqxmd.com/read/29166020/solid-digestion-of-demineralized-bone-as-a-method-to-access-potentially-insoluble-proteins-and-post-translational-modifications
#1
Timothy P Cleland
Bone proteomics is an expanding field for understanding protein changes associated with disease as well as characterizing and detecting proteins preserved in fossil bone. Most previous studies have utilized a protocol with demineralization and extraction approach to isolate and characterize proteins from bone. Through near-complete EDTA demineralization, followed by solid digestion of the remaining bone pseudomorph, a total of 92 protein accessions were detected from dog bone. In the EDTA, 14 unique proteins were found, including osteocalcin, an important bone protein...
November 22, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29146168/bone-marrow-derived-mesenchymal-stromal-cells-enhanced-by-platelet-rich-plasma-maintain-adhesion-to-scaffolds-in-arthroscopic-simulation
#2
Alexander R Hoberman, Carl Cirino, Mary Beth McCarthy, Mark P Cote, Leo Pauzenberger, Knut Beitzel, Augustus D Mazzocca, Felix Dyrna
PURPOSE: To assess the response of bone marrow-derived mesenchymal stromal cells (bMSCs) enhanced by platelet-rich plasma (PRP) in the setting of a normal human tendon (NHT), a demineralized bone matrix (DBM), and a fibrin scaffold (FS) with simulated arthroscopic mechanical washout stress. METHODS: Bone marrow was aspirated from the humeral head and concentrated. BMSCs were counted, plated, and grown to confluence. Cells were seeded onto 3 different scaffolds: (1) NHT, (2) DBM, and (3) FS...
November 13, 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/29140136/salt-glycerol-induced-hyperhydration-enhances-fluid-retention-more-than-salt-or-glycerol-induced-hyperhydration
#3
Eric D B Goulet, Adrien De La Flore, Félix A Savoie, Jonathan Gosselin
Hyperhydration has been demonstrated to improve work capacity and cardiovascular and thermoregulatory functions, enhance orthostatic tolerance, slow or neutralize bone demineralization and decrease post-dive bubble formation. Adding sodium or glycerol to a hyperhydration solution optimizes fluid retention. Sodium and glycerol produce their effect through different physiological mechanisms. If combined into a hyperhydration solution their impact on fluid retention could potentially be greater than their singular effect...
November 15, 2017: International Journal of Sport Nutrition and Exercise Metabolism
https://www.readbyqxmd.com/read/29124078/demineralized-bone-matrix-to-augment-tendon-bone-healing-a-systematic-review
#4
Adam T Hexter, Catherine Pendegrass, Fares Haddad, Gordon Blunn
Background: Following injury to the rotator cuff and anterior cruciate ligament, a direct enthesis is not regenerated, and healing occurs with biomechanically inferior fibrous tissue. Demineralized bone matrix (DBM) is a collagen scaffold that contains growth factors and is a promising biological material for tendon and ligament repair because it can regenerate a direct fibrocartilaginous insertion via endochondral ossification. Purpose: To provide a comprehensive review of the literature investigating the use of DBM to augment tendon-bone healing in tendon repair and anterior cruciate ligament reconstruction (ACLR)...
October 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/29117238/tdf-and-quantitative-ultrasound-bone-quality-in-african-patients-on-second-line-art-anrs-12169-2lady-sub-study
#5
Firmin Nongodo Kabore, Sabrina Eymard-Duvernay, Jacques Zoungrana, Stéphanie Badiou, Guillaume Bado, Arsène Héma, Assane Diouf, Eric Delaporte, Sinata Koulla-Shiro, Laura Ciaffi, Amandine Cournil
BACKGROUND: Bone demineralization, which leads to osteoporosis and increased fracture risk, is a common metabolic disorder in HIV-infected individuals. In this study, we aimed to assess the change in bone quality using quantitative ultrasound (QUS) over 96 weeks of follow-up after initiation of second-line treatment, and to identify factors associated with change in bone quality. METHODS AND FINDINGS: In a randomized trial (ANRS 12169), TDF and PI-naïve participants failing standard first-line treatment, from Burkina Faso, Cameroon, and Senegal were randomized to receive either TDF/FTC/LPVr, ABC/ddI/LPVr or TDF/FTC/DRVr...
2017: PloS One
https://www.readbyqxmd.com/read/29113732/oxidative-stress-in-ageing-and-disease-development-studied-by-ft-ir-spectroscopy
#6
Jane Anastassopoulou, Maria Kyriakidou, Stylianos Kyriazis, Andreas F Mavrogenis, Vasiliki Mamareli, Ioannis Mamarelis, Marianna Petra, Efthymia Malesiou, Christoforos Kotoulas, Panagiota Kolovou, Emmanouil Koutoulakis, Athina Markouizou, Theophile Theophanides
FT-IR spectroscopy was used to investigate the effect of oxidative stress and to approach the mechanism on cancer bone demineralization, aortic valve mineralization and heterotopic ossification on disease development. The FT-IR spectra obtained from paediatric, adult bone and ex vivo irradiated adult healthy bone with a dose of 20Gy were compared with those of healthy bone. The increase of band intensity changes of vasCH2,vsCH2 in the region 3000-2850cm(-1) depended on aging, the disease progression and the dose of irradiation...
November 4, 2017: Mechanisms of Ageing and Development
https://www.readbyqxmd.com/read/29095788/is-bone-graft-or-guided-bone-regeneration-needed-when-placing-immediate-dental-implants-a-systematic-review
#7
Hania AlKudmani, Reham Al Jasser, Sebastiano Andreana
AIMS: To systematically review the effect and type of bone graft and guided bone regeneration around immediate implants on hard and soft tissue changes. METHODS: Three electronic databases were searched up to June 2015. Outcomes consisted of hard and soft tissue dimensional changes. RESULTS: Eight studies were included according to inclusion criteria. Immediate implants with bone grafting had superior soft tissue stability and preserved horizontal ridge dimension and buccal plate thickness, when compared to no grafting...
November 1, 2017: Implant Dentistry
https://www.readbyqxmd.com/read/29094007/soft-tissue-swelling-incidence-using-demineralized-bone-matrix-in-the-outpatient-setting
#8
Kingsley R Chin, Fabio J R Pencle, Jason A Seale, Juan M Valdivia
AIM: To assess use of demineralized bone matrix (DBM) use in anterior cervical discectomy and fusion (ACDF) in outpatient setting. METHODS: One hundred and forty-five patients with prospectively collected data undergoing single and two level ACDF with DBM packed within and anterior to polyetheretherketone (PEEK) cages. Two groups created, Group 1 (75) outpatients and control Group 2 (70) hospital patients. Prevertebral soft tissue swelling (PVSTS) was measured anterior to C2 and C6 on plain lateral cervical radiographs preoperatively and one week postoperatively and fusion assessed at two years...
October 18, 2017: World Journal of Orthopedics
https://www.readbyqxmd.com/read/29093779/comparison-of-clinical-and-radiological-outcomes-of-lumbar-interbody-fusion-using-a-combination-of-hydroxyapatite-and-demineralized-bone-matrix-and-autografts-for-lumbar-degenerative-spondylolisthesis
#9
Asrafi Rizki Gatam, Luthfi Gatam, Singkat Dohar Lumban Tobing
Study Design: Prospective, cohort, non-inferiority study. Purpose: This study evaluated the clinical and radiological outcomes of interbody fusion using a combination of demineralized bone matrix (DBM) and hydroxyapatite (HA). Overview of Literature: The use of autografts remains a gold standard in lumbar interbody fusion, but the limited availability and donor site morbidity encourages the use of bone substitutes. In addition to autografts, a combination of HA and DBM is being increasingly use for lumbar interbody fusion...
October 2017: Asian Spine Journal
https://www.readbyqxmd.com/read/29093738/comparative-effectiveness-of-bone-grafting-using-xenograft-freeze-dried-cortical-bovine-allograft-freeze-dried-cortical-new-zealand-white-rabbit-xenograft-hydroxyapatite-bovine-and-xenograft-demineralized-bone-matrix-bovine-in-bone-defect-of-femoral-diaphysis
#10
Ferdiansyah Mahyudin, Dwikora Novembri Utomo, Heri Suroto, Tri Wahyu Martanto, Mouli Edward, Imelda Lumban Gaol
Autogenous bone graft is gold standard in treating bone defects, but it might have difficulty in corporation and rejection reaction. This study is to compare the effectiveness among freeze-dried xenograft, freeze-dried allograft, hydroxyapatite xenograft, and demineralized bone matrix xenograft as bone graft to fill bone defect in femoral diaphysis of white rabbit. Thirty male New Zealand white rabbits were distributed into five groups. Bone defect was filled correspondingly with xenograft freeze-dried cortical bovine, allograft freeze-dried cortical New Zealand white rabbit, xenograft hydroxyapatite bovine, and xenograft demineralized bone matrix bovine...
2017: International Journal of Biomaterials
https://www.readbyqxmd.com/read/29081701/role-of-bone-grafts-and-bone-graft-substitutes-in-isolated-subtalar-joint-arthrodesis
#11
Ashish Shah, Sameer Naranje, Ibukunoluwa Araoye, Osama Elattar, Alexandre Leme Godoy-Santos, Cesar DE Cesar
OBJECTIVES: The purpose of this study was to compare union rates for isolated subtalar arthrodesis with and without the use of bone grafts or bone graft substitutes. METHODS: We retrospectively reviewed 135 subtalar fusions with a mean follow-up of 18 ± 14 months. The standard approach was used for all surgeries. Graft materials included b-tricalcium phosphate, demineralized bone matrix, iliac crest autograft and allograft, and allograft cancellous chips. Successful subtalar fusion was determined clinically and radiographically...
September 2017: Acta Ortopedica Brasileira
https://www.readbyqxmd.com/read/29080346/vegf-bmp-2-loaded-three-dimensional-model-for-enhanced-angiogenic-and-odontogenic-potential-of-dental-pulp-stem-cells
#12
H Aksel, Ş Öztürk, A Serper, K Ulubayram
AIM: To investigate the proliferation and differentiation potential of human dental pulp stem cells (DPSCs) in a three-dimensional culture model (TDM) by incorporation of VEGF and BMP-2. METHODOLOGY: TDM was established using fibrin gel (fg) as a soft tissue matrix and demineralized dentine disc (dd) as a hard tissue matrix. DPSCs and vascular endothelial growth factor (VEGF) were encapsulated in fibrin gel (fg-VEGF) and then inserted into bone morphogenetic protein (BMP-2) coated demineralized dentine discs (dd-BMP-2)...
October 28, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/29077506/a-rare-case-of-chondroblastoma-with-revision-after-graft-rejectiona-case-report
#13
Brandon Gumbiner, Elizabeth Jacobsen, Mary Stancukas, Ngan Nguyen
We present a rare case of calcaneal chondroblastoma with subsequent surgical revision after graft rejection in a 13-year-old boy. Complications were encountered after the injectable bone graft filler was placed in the calcaneus after curettage. With noted subsequent sinus tract formation, revision surgery was performed that involved dissection of the sinus tract, removal of bone void filler, and application of demineralized bone matrix sponge human allograft soaked in vancomycin-impregnated saline. Sixteen weeks after the revision surgical intervention, the patient resumed normal athletic activities without pain or restrictions...
September 2017: Journal of the American Podiatric Medical Association
https://www.readbyqxmd.com/read/29045937/adenovirus-mediated-expression-of-bmp-2-and-bfgf-in-bone-marrow-mesenchymal-stem-cells-combined-with-demineralized-bone-matrix-for-repair-of-femoral-head-osteonecrosis-in-beagle-dogs
#14
Wu-Xun Peng, Lei Wang
BACKGROUND: This study investigated the effect of using adenovirus-mediated expression of bone morphogenetic protein 2 (Ad-BMP-2) and basic fibroblast growth factor (bFGF) in bone marrow mesenchymal stem cells (BMSCs) in combination with a demineralized bone matrix (DBM) to repair osteonecrosis of the femoral head (ONFH) in Beagle dogs. METHODS: A total of 30 Beagle dogs were selected for the isolation of BMSCs, which were cultured and transfected with the recombinant adenovirus vector Ad-BMP2-bFGF-GFP (carrying BMP-2 and bFGF) or a control adenovirus plasmid (encoding green fluorescent protein (Ad-GFP))...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29034241/bone-mineral-density-after-weight-gain-in-160-patients-with-anorexia-nervosa
#15
Najate Achamrah, Moïse Coëffier, Pierre Jésus, Jocelyne Charles, Agnès Rimbert, Pierre Déchelotte, Sébastien Grigioni
Low bone mineral density (BMD) is a frequent complication in anorexia nervosa (AN). There are controversial points of views regarding the restoration of bone mineralization after recovery in AN. We aimed to assess changes of BMD at 3 years in patients with AN and to explore the relationships between body composition, physical activity, and BMD. Patients with AN were included from 2009 to 2011 in a first visit (T0) with evaluation of weight, height, body mass index (BMI), body composition [fat mass (FM) and fat-free mass], and BMD...
2017: Frontiers in Nutrition
https://www.readbyqxmd.com/read/29030467/metabolic-acidosis-and-subclinical-metabolic-acidosis-in-ckd
#16
Kalani L Raphael
Metabolic acidosis is not uncommon in CKD and is linked with bone demineralization, muscle catabolism, and higher risks of CKD progression and mortality. Clinical practice guidelines recommend maintaining serum total CO2 at ≥22 mEq/L to help prevent these complications. Although a definitive trial testing whether correcting metabolic acidosis improves clinical outcomes has not been conducted, results from small, single-center studies support this notion. Furthermore, biologic plausibility supports the notion that a subset of patients with CKD have acid-mediated organ injury despite having a normal serum total CO2 and might benefit from oral alkali before overt acidosis develops...
October 13, 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/29018937/local-effect-of-zoledronic-acid-on-new-bone-formation-in-posterolateral-spinal-fusion-with-demineralized-bone-matrix-in-a-murine-model
#17
Pawel Zwolak, Jan Farei-Campagna, Thorsten Jentzsch, Brigitte von Rechenberg, Clément M Werner
BACKGROUND: Posterolateral spinal fusion is a common orthopaedic surgery performed to treat degenerative and traumatic deformities of the spinal column. In posteriolateral spinal fusion, different osteoinductive demineralized bone matrix products have been previously investigated. We evaluated the effect of locally applied zoledronic acid in combination with commercially available demineralized bone matrix putty on new bone formation in posterolateral spinal fusion in a murine in vivo model...
October 10, 2017: Archives of Orthopaedic and Trauma Surgery
https://www.readbyqxmd.com/read/28992519/posterior-atlantoaxial-fusion-with-a-screw-rod-system-allograft-versus-iliac-crest-autograft
#18
Da-Geng Huang, Xin-Liang Zhang, Ding-Jun Hao, Cheng-Cheng Yu, Bai-Bing Mi, Qi-Ling Yuan, Bao-Rong He, Tuan-Jiang Liu, Hua Guo, Xiao-Dong Wang
OBJECTIVE: To compare the effectiveness of allograft and iliac crest autograft in atlantoaxial fusion. PATIENTS AND METHODS: Between January 2012 and December 2012, 41 consecutive patients underwent posterior atlantoaxial fusion with a screw-rod fixation system in our spine center. The choice to use allograft or iliac crest autograft was made by the patient himself or herself after being informed about the advantages and disadvantages of both methods. In the allograft group, we used mixed material of morcellized demineralized freeze-dried bone allograft and local autograft for posterior atlantoaxial fusion...
November 2017: Clinical Neurology and Neurosurgery
https://www.readbyqxmd.com/read/28986227/anterior-cervical-discectomy-with-instrumented-allograft-fusion-lordosis-restoration-and-comparison-of-functional-outcomes-among-patients-of-different-age-groups
#19
Dario Muzevic, Bruno Splavski, Frederick A Boop, Kenan I Arnautovic
OBJECTIVE: To investigate clinical parameters of anterior cervical discectomy and fusion (ACDF) treatment and outcomes using osseous allograft in different age groups, study postoperative results of restoring of lordosis, and evaluate the utility of bone allografts for ACDF, including graft subsidence. METHODS: We reviewed data from 154 patients having clinical symptoms and radiological signs of disc herniation and/or cervical spondylosis. Decompression was achieved through discectomy, osteophyte ablation, endplate drilling, and foraminotomy...
October 3, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28984044/an-engineered-tendon-ligament-bioscaffold-derived-from-decellularized-and-demineralized-cortical-bone-matrix
#20
Jie-Liang Yang, Xuan Yao, Quan Qing, Yi Zhang, Yan-Lin Jiang, Liang-Ju Ning, Jing-Cong Luo, Ting-Wu Qin
Demineralized bone matrix (DBM), as an extracellular matrix (ECM), has had limited use as a medical replacement although studies have reported a possibility for its use in tendon or ligament tissue engineering. To be an acid-extracted organic matrix, DBM contains much of bone protein, with a small amount of inorganic solids and some cell debris. However, cell debris is a critical factor that triggers inflammatory reaction in clinical reconstructions using ECM. In this study, we used a protocol incorporating the use of detergent with nuclease treatment to prepare decellularized DBM (DCDBM)...
October 6, 2017: Journal of Biomedical Materials Research. Part A
keyword
keyword
58462
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"