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Bone Demineralization

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https://www.readbyqxmd.com/read/29773374/electrospinning-and-wound-healing-activity-of-%C3%AE-chitin-extracted-from-cuttlefish-bone
#1
Hyeong-Seop Jung, Min Hee Kim, Ji Youn Shin, Se Ra Park, Ju-Young Jung, Won Ho Park
A nanofibrous β-chitin web was fabricated via electrospinning for use as a novel wound dressing material. β-chitin was extracted from cuttlefish bone using deproteinization and demineralization. First, cuttlefish bone was alkali-treated to remove the proteins and was then treated with the acid for demineralization. The extracted β-chitin was dissolved in formic acid as solvent to evaluate its electrospinnability, and the electrospinnability increased remarkably when β-chitin was blended with poly(ethylene oxide) (PEO) than without...
August 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29768538/demineralized-bone-matrix-and-calcium-phosphate-cement-in-bone-regeneration-in-rats
#2
Luiz Carlos Ferreira da Silva, Gabriela Granja Porto, Emanuel Sávio de Souza Andrade, José Rodrigues Laureano Filho
PURPOSE: To compare bone regeneration in critical-sized defects in rat calvarium using demineralized bone matrix and calcium phosphate cement. METHODS: Thirty Wistar rats were divided into 3 groups of 10 animals each. Two defects of 5-mm were made in the parietal bones of each animal. Group I had calcium phosphate cement placed in the experimental defect, Group II had filled with demineralized bone matrix and Group III had with the combination of the matrix and cement in equal parts...
April 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29745814/trip-1-in-the-extracellular-matrix-promotes-nucleation-of-calcium-phosphate-polymorphs
#3
Amsaveni Ramachandran, Kun He, Chun-Chieh Huang, Reza Shahbazian-Yassar, Tolou Shokuhfar, Anne George
In search for bone and dentin extracellular matrix (ECM) proteins, transforming growth factor beta receptor II interacting protein 1 (TRIP-1) was identified as a novel protein synthesized by osteoblasts and odontoblasts and exported to the ECM. TRIP-1 is a WD-40 (WD is Tryptophan-Aspartic acid dipeptide) protein that has been well recognized for its physiological role in the endoplasmic reticulum (ER). In the ER, TRIP-1 functions as an essential subunit of eukaryotic elongation initiation factor 3 and is involved in the protein translational machinery...
December 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29738994/protein-based-forensic-identification-using-genetically-variant-peptides-in-human-bone
#4
Katelyn Elizabeth Mason, Deon Anex, Todd Grey, Bradley Hart, Glendon Parker
Bone tissue contains organic material that is useful for forensic investigations and may contain preserved endogenous protein that can persist in the environment for extended periods of time over a range of conditions. Single amino acid polymorphisms in these proteins reflect genetic information since they result from non-synonymous single nucleotide polymorphisms (SNPs) in DNA. Detection of genetically variant peptides (GVPs) - those peptides that contain amino acid polymorphisms - in digests of bone proteins allows for the corresponding SNP alleles to be inferred...
April 22, 2018: Forensic Science International
https://www.readbyqxmd.com/read/29692564/effects-of-mangosteen-peel-extract-combined-with-demineralized-freeze-dried-bovine-bone-xenograft-on-osteocalcin-collagen-1-and-osteoblast-as-alveolar-bone-regeneration-in-socket-preservation
#5
Utari Kresnoadi, Tika Raharjo, Rostiny Rostiny
Background: Tooth extraction will provoke changes in alveolar bone morphology and dimensions. Postextraction bone resorption can lead to significant problems for restorative dentistry. Therefore, the extracted tooth socket needs to be preserved to reduce alveolar ridge bone resorption. This research aimed to analyze the expression and levels of osteocalcin, collagen 1, and osteoblasts in extracted tooth sockets filled with a combination of mangosteen peel extract and demineralized freeze-dried bovine bone xenograft (DFDBBX)...
April 2018: Journal of Indian Prosthodontic Society
https://www.readbyqxmd.com/read/29685704/assessment-of-reamer-irrigator-aspirator-system-ria-filtrate-for-its-osteoinductive-potential-in-a-validated-animal-model
#6
Alex R Wessel, Brett D Crist, James P Stannard, Gregory J Della Rocca, Aaron M Stoker, Chantelle C Bozynski, Cristi R Cook, Keiichi Kuroki, Carin E Ahner, James L Cook
PURPOSE: Previous studies indicate that Reamer Irrigator Aspirator (RIA) filtrate contains proteins that have the potential to stimulate bone healing. This study aimed to determine the osteoinductive capabilities of RIA filtrate in a validated in vivo model. METHODS: With Institutional Review Board approval, RIA filtrates from 9 patients were collected. The filtrate was processed to remove cells and inorganic particles. A portion of each sample was set aside for protein analysis while the remainder was lyophilized and prepared for implantation...
April 17, 2018: Injury
https://www.readbyqxmd.com/read/29682530/efficacy-of-rhbmp-2-loaded-pcl-%C3%AE-tcp-bdecm-scaffold-fabricated-by-3d-printing-technology-on-bone-regeneration
#7
Eun-Bin Bae, Keun-Ho Park, Jin-Hyung Shim, Ho-Yun Chung, Jae-Won Choi, Jin-Ju Lee, Chang-Hwan Kim, Ho-Jun Jeon, Seong-Soo Kang, Jung-Bo Huh
This study was undertaken to evaluate the effect of 3D printed polycaprolactone (PCL)/ β -tricalcium phosphate ( β -TCP) scaffold containing bone demineralized and decellularized extracellular matrix (bdECM) and human recombinant bone morphogenetic protein-2 (rhBMP-2) on bone regeneration. Scaffolds were divided into PCL/ β -TCP, PCL/ β -TCP/bdECM, and PCL/ β -TCP/bdECM/BMP groups. In vitro release kinetics of rhBMP-2 were determined with respect to cell proliferation and osteogenic differentiation. These three reconstructive materials were implanted into 8 mm diameter calvarial bone defect in male Sprague-Dawley rats...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29677404/hybrid-scaffolds-of-mg-alloy-mesh-reinforced-polymer-extracellular-matrix-composite-for-critical-sized-calvarial-defect-reconstruction
#8
Yingqi Chen, Sang-Ho Ye, Hideyoshi Sato, Yang Zhu, Vesselin Shanov, Tarannum Tiasha, Antonio D'Amore, Samuel Luketich, Guojiang Wan, William R Wagner
The challenge of developing scaffolds to reconstruct critical-sized calvarial defects without the addition of high levels of exogenous growth factor remains relevant. Both osteogenic regenerative efficacy as well as suitable mechanical properties for the temporary scaffold system are of importance. In this study, a Mg alloy mesh reinforced polymer/demineralized bone matrix (DBM) hybrid scaffold was designed where the hybrid scaffold was fabricated by a concurrent electrospinning/electrospraying of poly(lactic-co-glycolic) (PLGA) polymer and DBM suspended in hyaluronic acid (HA)...
April 20, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29656495/randomized-controlled-clinical-study-assessing-two-membranes-for-guided-bone-regeneration-of-peri-implant-bone-defects-3-year-results
#9
Tobias Basler, Nadja Naenni, David Schneider, Christoph H F Hämmerle, Ronald E Jung, Daniel S Thoma
OBJECTIVES: To assess two- and three-dimensional changes of the peri-implant tissues as well as clinical, biological, and radiological outcomes of implants having been treated with resorbable or nonresorbable membranes at 3 years. MATERIALS AND METHODS: Twenty-three patients were re-examined after having received a single-tooth implant in the esthetic zone in conjunction with guided bone regeneration (GBR) using either a resorbable (RES) or a titanium-reinforced nonresorbable membrane (N-RES) and demineralized bovine bone mineral...
April 15, 2018: Clinical Oral Implants Research
https://www.readbyqxmd.com/read/29655532/evaluation-of-different-therapeutic-carnoy-s-formulations-on-hard-human-tissues-a-raman-microspectroscopy-microhardness-and-scanning-electron-microscopy-study
#10
Francisco Samuel Rodrigues Carvalho, Victor Pinheiro Feitosa, Paulo Goberlânio de Barros Silva, Eduardo Costa Studart Soares, Thyciana Rodrigues Ribeiro, Cristiane Sá Roriz Fonteles, Fábio Wildson Gurgel Costa
PURPOSE: To evaluate different therapeutic Carnoy's solution formulations on hard human tissues. MATERIALS AND METHODS: An in vitro study was performed with human teeth (n = 36) and bone fragments (n = 18), randomly divided into two experimental groups (Group I = Carnoy solution with chloroform; Group II = Carnoy solution without chloroform) and a control group (saline solution). The groups were subdivided into pre-conditioning, post-conditioning, and post-conditioning with saline washing...
February 21, 2018: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/29629724/born-in-auschwitz-and-survived-a-triumph-over-murderers
#11
George M Weisz, Konrad Kwiet
The discovery of Jewish babies who were born in Nazi concentration camps and survived seems miraculous, but this phenomenon did occur toward the end of World War II. The lives of a small group of mothers and surviving children are of both historical and medical interests. Their survival shows additional support for the hypothesis that maternal nutrition can induce metabolic syndrome and bone demineralization in their offspring. Information obtained through direct contact with some of the surviving children is the basis for this article...
April 2018: Israel Medical Association Journal: IMAJ
https://www.readbyqxmd.com/read/29613862/comparative-histomorphometric-analysis-of-maxillary-sinus-augmentation-with-deproteinized-bovine-bone-and-demineralized-particulate-human-tooth-graft-an-experimental-study-in-rabbits
#12
Xiping Xu, Dong-Seok Sohn, Hyung-Gyun Kim, Sang-Jae Lee, Yong-Suk Moon
PURPOSE: The aim of this animal study is to evaluate, by histomorphometric analysis, new bone formation in rabbit maxillary sinuses with Bio-Oss and demineralized particulate human tooth graft. MATERIALS AND METHODS: Bilateral sinus augmentation procedures were performed in 8 adult male rabbits. After preparation of replaceable bony windows on the lateral wall of the nasal cavity with a piezoelectric surgical device, deproteinized bovine graft (Bio-Oss) was grafted in the new compartment of the maxillary sinus after elevation of the sinus membrane in the control group...
April 2, 2018: Implant Dentistry
https://www.readbyqxmd.com/read/29600260/current-concepts-in-scaffolding-for-bone-tissue-engineering
#13
REVIEW
Toktam Ghassemi, Azadeh Shahroodi, Mohammad H Ebrahimzadeh, Alireza Mousavian, Jebraeel Movaffagh, Ali Moradi
Bone disorders are of significant worry due to their increased prevalence in the median age. Scaffold-based bone tissue engineering holds great promise for the future of osseous defects therapies. Porous composite materials and functional coatings for metallic implants have been introduced in next generation of orthopedic medicine for tissue engineering. While osteoconductive materials such as hydroxyapatite and tricalcium phosphate ceramics as well as some biodegradable polymers are suggested, much interest has recently focused on the use of osteoinductive materials like demineralized bone matrix or bone derivatives...
March 2018: Archives of Bone and Joint Surgery
https://www.readbyqxmd.com/read/29600207/treating-a-recalcitrant-non-union-of-the-radius-using-autogenous-bone-equine-bone-paste-equine-demineralized-bone-matrix-platelet-rich-plasma-and-bone-marrow-aspirate-a-case-report
#14
Ferdinando Da Rin De Lorenzo
Introduction: Non-unions at forearms are usually challenging and difficult to treat. If additionally, an infection is present, reconstructive surgery should be planned only after full debridement, antibiotic treatment, and confirmation, based on clinical observation and laboratory tests that the infection has subsided. Bone grafting may be required for reconstruction. The use of autogenous bone calls for a second surgical site with an increased risk of morbidity. Using bone substitutes may reduce the need for autogenous bone...
November 2017: Journal of Orthopaedic Case Reports
https://www.readbyqxmd.com/read/29594993/-experimental-study-of-demineralized-dentin-matrix-on-osteoinduction-and-related-cells-identification
#15
Yang Shengyin, Chen Ping, Bao Jibo, Ding Yixin, Zou Jinyang, Xie Zhigang
OBJECTIVE: The aim of this study was to explore the theoretical framework of cells and the forms of osteogenesis in the mechanism by which demineralized dentin matrix (DDM) induces osteogenesis. METHODS: A total of 24 New Zealand rabbits were used in this study. A total of 4 erector spinae bags were created in each animal. A total of 3 erector spinae bags were implanted with DDM by random selection, whereas the remaining one erector spinae bag was not implanted with DDM...
February 1, 2018: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29590225/periodontal-regenerative-treatment-of-intrabony-defects-in-the-esthetic-zone-using-modified-vestibular-incision-subperiosteal-tunnel-access-m-vista
#16
Babak Najafi, Parisa Kheirieh, Alireza Torabi, Emil G Cappetta
Periodontal regenerative treatment of intrabony defects in the esthetic zone often poses a significant challenge to clinicians. Various materials and techniques have been proposed to achieve periodontal regeneration. This article presents modified vestibular incision subperiosteal tunnel access (M-VISTA) for treatment of intrabony defects in the esthetic zone. Two cases were treated using the proposed approach with enamel matrix derivatives and demineralized freeze-dried bone allograft to optimize the regenerative outcomes without affecting the soft tissue profile in the esthetic zone...
March 28, 2018: International Journal of Periodontics & Restorative Dentistry
https://www.readbyqxmd.com/read/29571671/impact-of-particulate-deproteinized-bovine-bone-mineral-and-porous-titanium-granules-on-early-stability-and-osseointegration-of-dental-implants-in-narrow-marginal-circumferential-bone-defects
#17
A Verket, S P Lyngstadaas, H Tiainen, H J Rønold, J C Wohlfahrt
The use of two particulate bone graft substitute materials in experimental narrow marginal peri-implant bone defects was investigated with respect to early bone healing and implant stability. Porous titanium granules, oxidized white porous titanium granules (WPTG), and demineralized bovine bone mineral (DBBM) were characterized in vitro, after which the two latter materials were tested in experimental peri-implant bone defects in six minipigs, with empty defects as control. After mandibular premolar extraction, the top 5mm of the alveoli were widened to 6mm in diameter, followed by the placement of six implants, three on each side, in each pig...
March 20, 2018: International Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/29570520/stranger-things-a-whimsical-account-of-a-demineralized-bone-matrix-study-with-unexpected-results
#18
William S Pietrzak
No abstract text is available yet for this article.
March 22, 2018: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/29551486/parathyroid-hormone-gene-activated-matrix-with-dfdba-collagen-composite-matrix-enhances-bone-regeneration-in-rat-calvarial-bone-defects
#19
Po-Hui Lee, Tu-Lai Yew, Yu-Lin Lai, Shyh-Yuan Lee, Hen-Li Chen
BACKGROUND: Gene-activated matrix (GAM) induces sustained local production of growth factors to promote tissue regeneration. GAM contains a plasmid DNA (pDNA) encoding target proteins that is physically entrapped within a biodegradable matrix carrier. GAM with a pDNA encoding the first 34 amino acids of parathyroid hormone (PTH 1-34) and a collagen matrix enhances bone regeneration in long bone defects. Demineralized freeze-dried bone allograft (DFDBA) is a widely used osteoinductive bone graft...
March 15, 2018: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/29550606/the-local-cytokine-and-growth-factor-response-to-rhbmp-2-after-spinal-fusion
#20
John D Koerner, Dessislava Z Markova, Greg D Schroeder, Brian P Calio, Anuj Shah, Corbin W Brooks, Alexander R Vaccaro, D Greg Anderson, Chris K Kepler
BACKGROUND CONTEXT: The systemic response regarding cytokine expression after application of recombinant human Bone Morphogenetic Protein-2 (rhBMP-2) in a rat spinal fusion model has recently been defined1 , but the local response has not. Defining the local cytokine and growth factor response at the fusion site will help explain the roles of these molecules in the fusion process, as well as that of rhBMP-2. Our hypothesis is that application of rhBMP-2 to the fusion site will alter the local levels of cytokines and growth factors throughout the fusion process, in a manner that is different than the systemic response given the tissue-specific effects of rhBMP-2...
March 14, 2018: Spine Journal: Official Journal of the North American Spine Society
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