Rebecca S Tooze, Kerry A Miller, Sigrid M A Swagemakers, Eduardo Calpena, Simon J McGowan, Odile Boute, Corinne Collet, David Johnson, Fanny Laffargue, Nicole de Leeuw, Jenny V Morton, Peter Noons, Charlotte W Ockeloen, Julie M Phipps, Tiong Yang Tan, Andrew T Timberlake, Clemence Vanlerberghe, Steven A Wall, Astrid Weber, Louise C Wilson, Elaine H Zackai, Irene M J Mathijssen, Stephen R F Twigg, Andrew O M Wilkie
PURPOSE: Studies previously implicated PRRX1 in craniofacial development, including demonstration of murine Prrx1 expression in the pre-osteogenic cells of the cranial sutures. We investigated the role of heterozygous missense and loss-of-function variants in PRRX1 associated with craniosynostosis. METHODS: Trio-based genome, exome or targeted sequencing were used to screen PRRX1 in patients with craniosynostosis; immunofluorescence analyses were used to assess nuclear localization of wild-type and mutant proteins...
May 5, 2023: Genetics in Medicine: Official Journal of the American College of Medical Genetics