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https://www.readbyqxmd.com/read/27751855/sucrose-delays-senescence-and-preserves-functional-compounds-in-asparagus-officinalis-l
#1
Me-Hea Park
The high metabolic rate of harvested asparagus spears (Asparagus officinalis L.) causes rapid deterioration. To extend shelf life, we investigated the effect of sucrose treatment on asparagus during storage. Asparagus spears were treated with 3%, 5%, and 10% sucrose and stored at 2 °C for 20 h. Cellular respiration decreased, but other processes were unaltered by exogenous sucrose. The overall appearance of asparagus treated with 3% sucrose and stored at 2 °C for 18 days was rated as good and excellent, unlike that of untreated spears...
October 14, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27696667/the-anti-ageing-hormone-klotho-induces-nrf2-mediated-antioxidant-defences-in-human-aortic-smooth-muscle-cells
#2
Giuseppe Maltese, Paraskevi-Maria Psefteli, Benedetta Rizzo, Salil Srivastava, Luigi Gnudi, Giovanni E Mann, Richard C M Siow
Vascular ageing in conditions such as atherosclerosis, diabetes and chronic kidney disease, is associated with the activation of the renin angiotensin system (RAS) and diminished expression of antioxidant defences mediated by the transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2). The anti-ageing hormone klotho promotes longevity and protects against cardiovascular and renal diseases. Klotho has been shown to activate Nrf2 and attenuate oxidative damage in neuronal cells, however, the mechanisms by which it protects against vascular smooth muscle cell VSMC dysfunction elicited by Angiotensin II (AngII) remain to be elucidated...
October 3, 2016: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/26923389/evaluation-of-toxicological-biomarkers-in-secreted-proteins-of-hepg2-cells-exposed-to-2-3-7-8-tetrachlorodibenzo-p-dioxin-and-their-expressions-in-the-plasma-of-rats-and-incineration-workers
#3
Sohee Phark, So-Young Park, Yoon-Seok Chang, Seonyoung Choi, Ji-youn Lim, Yoonjin Kim, Jong Bok Seo, Woon-Won Jung, Donggeun Sul
Toxicological biomarkers of 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) were investigated in proteins secreted by HepG2 cells and their expression levels were determined in the plasma of rats exposed to 2,3,7,8-TCDD and in the plasma of incineration workers exposed to dioxins. HepG2 cells were treated with various concentrations of 2,3,7,8-TCDD (0, 0.25, 0.5, 1, 2.5, 5, 10, 25 nM) for 24 or 48 h. MTT and Comet assays were performed to determine cytotoxicities and genotoxicities to select exposure concentrations for the proteomic analysis of proteins secreted by 2,3,7,8-TCDD-treated cells...
May 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/26378793/suppression-of-hydroxycinnamate-network-formation-in-cell-walls-of-rice-shoots-grown-under-microgravity-conditions-in-space
#4
Kazuyuki Wakabayashi, Kouichi Soga, Takayuki Hoson, Toshihisa Kotake, Takashi Yamazaki, Akira Higashibata, Noriaki Ishioka, Toru Shimazu, Keiji Fukui, Ikuko Osada, Haruo Kasahara, Motoshi Kamada
Network structures created by hydroxycinnamate cross-links within the cell wall architecture of gramineous plants make the cell wall resistant to the gravitational force of the earth. In this study, the effects of microgravity on the formation of cell wall-bound hydroxycinnamates were examined using etiolated rice shoots simultaneously grown under artificial 1 g and microgravity conditions in the Cell Biology Experiment Facility on the International Space Station. Measurement of the mechanical properties of cell walls showed that shoot cell walls became stiff during the growth period and that microgravity suppressed this stiffening...
2015: PloS One
https://www.readbyqxmd.com/read/26189658/fibrillin-1-regulates-skeletal-stem-cell-differentiation-by-modulating-tgf%C3%AE-activity-within-the-marrow-niche
#5
Silvia Smaldone, Nicholas P Clayton, Maria del Solar, Gemma Pascual, Seng H Cheng, Bruce M Wentworth, Mitchell B Schaffler, Francesco Ramirez
A full understanding of the microenvironmental factors that control the activities of skeletal stem cells (also known as mesenchymal stem cells [MSCs]) in the adult bone marrow holds great promise for developing new therapeutic strategies to mitigate age-related diseases of bone and cartilage degeneration. Bone loss is an understudied manifestation of Marfan syndrome, a multisystem disease associated with mutations in the extracellular matrix protein and TGFβ modulator fibrillin-1. Here we demonstrate that progressive loss of cancellous bone in mice with limbs deficient for fibrillin-1 (Fbn1(Prx1-/-) mice) is accounted for by premature depletion of MSCs and osteoprogenitor cells combined with constitutively enhanced bone resorption...
January 2016: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/26160718/chlorogenic-acid-prevents-acetaminophen-induced-liver-injury-the-involvement-of-cyp450-metabolic-enzymes-and-some-antioxidant-signals
#6
Chun Pang, Yu-chen Sheng, Ping Jiang, Hai Wei, Li-li Ji
Chlorogenic acid (CGA), a polyphenolic compound, is abundant in fruits, dietary vegetables, and some medicinal herbs. This study investigated the prevention of CGA against acetaminophen (AP)-induced hepatotoxicity and its engaged mechanisms. CGA reversed the decreased cell viability induced by AP in L-02 cells in vitro. In addition, CGA reduced the AP-induced increased serum levels of alanine/aspartate aminotransferase (ALT/AST) in vivo. The effect of CGA on cytochrome P450 (CYP) enzymatic (CYP2E1, CYP1A2, and CYP3A4) activities showed that CGA caused very little inhibition on CYP2E1 and CYP1A2 enzymatic activities, but not CYP3A4...
July 2015: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/26159064/glutathione-is-the-resolving-thiol-for-thioredoxin-peroxidase-activity-of-1-cys-peroxiredoxin-without-being-consumed-during-the-catalytic-cycle
#7
José Rafael Pedrajas, Brian McDonagh, Francisco Hernández-Torres, Antonio Miranda-Vizuete, Raúl González-Ojeda, Emilia Martínez-Galisteo, C Alicia Padilla, José Antonio Bárcena
AIMS: A three-step catalytic cycle is common to all peroxiredoxins (Prxs), despite structural and kinetic differences. The second step in 1-Cys type Prxs is a matter of debate since they lack an additional cysteine to play the resolving role, as happens with the 2-Cys Prxs. The aim of this study was to elucidate the role of glutathione (GSH) in the thioredoxin-dependent peroxidase activity of Saccharomyces cerevisiae mitochondrial Prx1p, a 1-Cys type Prx. RESULTS: The peroxidatic Cys91 residue of two Prx1p peptides can be linked by a disulfide, which can be reduced by thioredoxin and by GSH (Km=6...
January 20, 2016: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/26143575/effects-of-benzalkonium-chloride-proxel-lv-p3-hypochloran-triton-x-100-and-dowfax-63n10-on-anaerobic-digestion-processes
#8
German Antonio Enriquez Flores, Ioannis A Fotidis, Dimitar Borisov Karakashev, Kasper Kjellberg, Irini Angelidaki
In this study, the individual and synergistic toxicity of the following xenobiotics: Benzalkonium Chloride (BKC), Proxel LV (PRX), P3 Hypochloran (HPC), Triton X-100 (TRX), and DOWFAX 63N10 (DWF), on anaerobic digestion (AD) process, was assessed. The experiments were performed in batch and continuous (up-flow anaerobic sludge blanket, UASB) reactors with biochemical-industrial wastewater, as substrate. In batch experiments, half-maximal inhibitory concentrations (IC50) for the tested xenobiotics were found to be 13...
October 2015: Bioresource Technology
https://www.readbyqxmd.com/read/26117319/thioredoxin-1-peroxiredoxin-1-as-sensors-of-oxidative-stress-mediated-by-nadph-oxidase-activity-in-atherosclerosis
#9
Julio Madrigal-Matute, Carlos-Ernesto Fernandez-Garcia, Luis Miguel Blanco-Colio, Elena Burillo, Ana Fortuño, Roxana Martinez-Pinna, Patricia Llamas-Granda, Oscar Beloqui, Jesus Egido, Guillermo Zalba, José Luis Martin-Ventura
To assess the potential association between TRX-1/PRX-1 and NADPH oxidase (Nox) activity in vivo and in vitro, TRX-1/PRX-1 levels were assessed by ELISA in 84 asymptomatic subjects with known phagocytic NADPH oxidase activity and carotid intima-media thickness (IMT). We found a positive correlation between TRX-1/PRX-1 and NADPH oxidase-dependent superoxide production (r=0.48 and 0.47; p<0.001 for both) and IMT (r=0.31 and 0.36; p<0.01 for both) adjusted by age and sex. Moreover, asymptomatic subjects with plaques have higher PRX-1 and TRX plasma levels (p<0...
September 2015: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/26085437/autoregulation-monitoring-and-outcome-prediction-in-neurocritical-care-patients-does-one-index-fit-all
#10
Bernhard Schmidt, Matthias Reinhard, Vesna Lezaic, Damian D McLeod, Marco Weinhold, Heinz Mattes, Jürgen Klingelhöfer
Indexes PRx and Mx have been formerly introduced to assess cerebral autoregulation and have been shown to be associated with 3-month clinical outcome. In a mixed cohort of neurocritical care patients, we retrospectively investigated the impact of selected clinical characteristics on this association. Forty-one patients (18-77 years) with severe traumatic (TBI, N = 20) and non-traumatic (N = 21) brain injuries were studied. Cerebral blood flow velocity, arterial blood pressure and intracranial pressure were repeatedly recorded during 1-h periods...
June 2016: Journal of Clinical Monitoring and Computing
https://www.readbyqxmd.com/read/26079888/sod1-deficiency-induces-the-systemic-hyperoxidation-of-peroxiredoxin-in-the-mouse
#11
Takujiro Homma, Satoshi Okano, Jaeyong Lee, Junitsu Ito, Noriyuki Otsuki, Toshihiro Kurahashi, Eun Sil Kang, Osamu Nakajima, Junichi Fujii
A deficiency of superoxide dismutase 1 (SOD1) or peroxiredoxin (Prx) 2 causes anemia in mice due to elevated oxidative stress. In the current study, we investigated whether intrinsic oxidative stress caused by a SOD1 deficiency affected the redox status of Prx2 and other isoforms in red blood cells (RBCs) and several organs of mice. We observed a marked elevation in hyperoxidized Prx2 levels in RBCs from SOD1-deficient mice. Hyperoxidized Prx2 reportedly undergoes a rhythmic change in isolated RBCs under culture conditions...
August 7, 2015: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/26051873/preclinical-and-first-in-human-evaluation-of-prx-105-a-pegylated-plant-derived-recombinant-human-acetylcholinesterase-r
#12
Jacob Atsmon, Einat Brill-Almon, Carmit Nadri-Shay, Raul Chertkoff, Sari Alon, Dimitri Shaikevich, Inna Volokhov, Kirsten Y Haim, Daniel Bartfeld, Avidor Shulman, Ilya Ruderfer, Tehila Ben-Moshe, Orit Shilovitzky, Hermona Soreq, Yoseph Shaaltiel
PRX-105 is a plant-derived recombinant version of the human 'read-through' acetylcholinesterase splice variant (AChE-R). Its active site structure is similar to that of the synaptic variant, and it displays the same affinity towards organophosphorus (OP) compounds. As such, PRX-105 may serve as a bio-scavenger for OP pesticides and chemical warfare agents. To assess its potential use in prophylaxis and treatment of OP poisoning we conducted several preliminary tests, reported in this paper. Intravenous (IV) PRX-105 was administered to mice either before or after exposure to an OP toxin...
September 15, 2015: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/25990085/autoantibody-signatures-combined-with-epstein-barr-virus-capsid-antigen-iga-as-a-biomarker-panel-for-the-detection-of-nasopharyngeal-carcinoma
#13
COMPARATIVE STUDY
Yu-Hui Peng, Yi-Wei Xu, Li-Sheng Huang, Tian-Tian Zhai, Li-Hua Dai, Si-Qi Qiu, Yu-Su Yang, Wei-Zheng Chen, Li-Qun Zhang, En-Min Li, Li-Yan Xu
Nasopharyngeal carcinoma (NPC) is prevalent in Southern China and Southeast Asia, and autoantibody signatures may improve early detection of NPC. In this study, serum levels of autoantibodies against a panel of six tumor-associated antigens (p53, NY-ESO-1, MMP-7, Hsp70, Prx VI, and Bmi-1) and Epstein-Barr virus capsid antigen-IgA (VCA-IgA) were tested by enzyme-linked immunosorbent assay in a training set (220 NPC patients and 150 controls) and validated in a validation set (90 NPC patients and 68 controls)...
August 2015: Cancer Prevention Research
https://www.readbyqxmd.com/read/25971604/a-novel-1-cys-thioredoxin-peroxidase-gene-in-apis-cerana-cerana-characterization-of-acctpx4-and-its-role-in-oxidative-stresses
#14
Yifeng Huaxia, Fang Wang, Yan Yan, Feng Liu, Hongfang Wang, Xingqi Guo, Baohua Xu
Thioredoxin peroxidase (Tpx), also named peroxiredoxin (Prx), is an important peroxidase that can protect organisms against stressful environments. AccTpx4, a 1-Cys thioredoxin peroxidase gene from the Chinese honey bee Apis cerana cerana, was cloned and characterized. The AccTpx4 gene encodes a protein that is predicted to contain the conserved PVCTTE motif from 1-Cys peroxiredoxin. Quantitative real-time PCR (Q-PCR) and Western blotting revealed that AccTpx4 was induced by various oxidative stresses, such as cold, heat, insecticides, H(2)O(2), and HgCl(2)...
July 2015: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/25955690/differences-in-the-protein-expression-levels-of-trx2-and-prx3-in-the-hippocampal-ca1-region-between-adult-and-aged-gerbils-following-transient-global-cerebral-ischemia
#15
Choong Hyun Lee, Joon Ha Park, Jeong-Hwi Cho, Ji Hyeon Ahn, Eun Joo Bae, Moo-Ho Won
The thioredoxin (Trx) and peroxiredoxin (Prx) redox system is associated with neuronal damage and neuroprotective effects via the regulation of oxidative stress in brain ischemia. In the present study, ischemia-induced changes in the protein expression levels of Trx2 and Prx3 in the stratum pyramidale (SP) of the hippocampal CA1 region were investigated in adult and aged gerbils, subjected to 5 min of transient global cerebral ischemia, using immunohistochemistry and western blot analysis. In the adult ischemia-group, minimal Trx2 immunoreactivity was detected in the SP 2 days after ischemia-reperfusion...
August 2015: Molecular Medicine Reports
https://www.readbyqxmd.com/read/25935550/high-intensity-of-cytoplasmic-peroxiredoxin-vi-expression-is-associated-with-adverse-outcome-in-diffuse-large-b-cell-lymphoma-independently-of-international-prognostic-index
#16
Milla Elvi Linnea Kuusisto, Kirsi-Maria Haapasaari, Taina Turpeenniemi-Hujanen, Esa Jantunen, Ylermi Soini, Pekka Peroja, Risto Bloigu, Peeter Karihtala, Outi Kuittinen
AIMS: Diffuse large B-cell lymphoma (DLBCL) is an aggressive and potentially fatal disease. Prediction of risk of relapse is based on clinical markers. There is a need for more accurate biomarkers to select patients for more aggressive first-line treatments. Peroxiredoxins (Prxs) are a family of potent antioxidant proteins. Their prognostic role in DLBCL is unknown. METHODS: Altogether, 103 diagnostic biopsy samples from patients with DLBCL were immunohistochemically stained for Prxs I, II, III, V and VI...
July 2015: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/25934084/a-potential-antioxidant-enzyme-belonging-to-the-atypical-2-cys-peroxiredoxin-subfamily-characterized-from-rock-bream-oplegnathus-fasciatus
#17
Kasthuri Saranya Revathy, Navaneethaiyer Umasuthan, Ilson Whang, Hyung-Bok Jung, Bong-Soo Lim, Bo-Hye Nam, Jehee Lee
Peroxiredoxins (Prxs), a diverse family of antioxidant enzymes, exert their antioxidant function through which different peroxide species are detoxified. This study describes both structural and functional characterization of a mitochondrial Prx identified in rock bream, Oplegnathus fasciatus (RbPrx5). The ORF (573 bp) of RbPrx5 encoded a protein of 190 amino acids (20 kDa) containing a putative mitochondrial targeting sequence (residues 1-20) and a thioredoxin-2 motif (residues 31-190) and three conserved Cys residues...
September 2015: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/25889122/molecular-characterization-and-functional-analysis-of-a-peroxiredoxin-1-cdna-from-golden-pompano-trachinotus-ovatus
#18
Long Wang, Huayang Guo, Nan Zhang, Zhenhua Ma, Shigui Jiang, Dianchang Zhang
Peroxiredoxin 1 (Prx 1) is an important antioxidant protein that can protect organisms against the toxicity of reactive oxygen species. In this study, a full-length Prx 1 cDNA sequence (ToPrx 1) was identified from golden pompano (Trachinotus ovatus). The ToPrx 1 cDNA was 1049 base pairs (bp) long and contained a 5'-untranslated region (UTR) of 127 nucleotides, a 3'-UTR of 328 nucleotides, and a 594 bp open reading frame (ORF) encoding a 197 amino acid polypeptide. The ToPrx 1 protein showed strong homology (79-91%) with Prx 1 proteins from other species and contained the conserved Prx domain and the signature of the peroxidase catalytic center...
August 2015: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/25881547/peroxynitrite-and-hydrogen-peroxide-elicit-similar-cellular-stress-responses-mediated-by-the-ccp1-sensor-protein
#19
Dorival Martins, Iolie Bakas, Kelly McIntosh, Ann M English
Peroxynitrite [ONOO(H)] is an oxidant associated with deleterious effects in cells. Because it is an inorganic peroxide that reacts rapidly with peroxidases, we speculated that cells may respond to ONOO(H) and H2O2 challenge in a similar manner. We exposed yeast cells to SIN-1, a well-characterized ONOO(H) generator, and observed stimulation of catalase and peroxiredoxin (Prx) activities. Previously, we reported that H2O2 challenge increases these activities in wild-type cells and in cells producing the hyperactive mutant H2O2 sensor Ccp1(W191F) but not in Ccp1-knockout cells (ccp1Δ)...
August 2015: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/25852696/induction-of-protective-immune-responses-against-schistosomiasis-haematobium-in-hamsters-and-mice-using-cysteine-peptidase-based-vaccine
#20
Hatem Tallima, John P Dalton, Rashika El Ridi
One of the major lessons we learned from the radiation-attenuated cercariae vaccine studies is that protective immunity against schistosomiasis is dependent on the induction of T helper (Th)1-/Th2-related immune responses. Since most schistosome larval and adult-worm-derived molecules used for vaccination uniformly induce a polarized Th1 response, it was essential to include a type 2 immune response-inducing molecule, such as cysteine peptidases, in the vaccine formula. Here, we demonstrate that a single subcutaneous injection of Syrian hamsters with 200 μg active papain, 1 h before percutaneous exposure to 150 cercariae of Schistosoma haematobium, led to highly significant (P < 0...
2015: Frontiers in Immunology
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