keyword
MENU ▼
Read by QxMD icon Read
search

nanobodies

keyword
https://www.readbyqxmd.com/read/28915239/structural-basis-for-the-high-specificity-of-a-trypanosoma-congolense-immunoassay-targeting-glycosomal-aldolase
#1
Joar Pinto, Steven Odongo, Felicity Lee, Vaiva Gaspariunaite, Serge Muyldermans, Stefan Magez, Yann G-J Sterckx
BACKGROUND: Animal African trypanosomosis (AAT) is a neglected tropical disease which imposes a heavy burden on the livestock industry in Sub-Saharan Africa. Its causative agents are Trypanosoma parasites, with T. congolense and T. vivax being responsible for the majority of the cases. Recently, we identified a Nanobody (Nb474) that was employed to develop a homologous sandwich ELISA targeting T. congolense fructose-1,6-bisphosphate aldolase (TcoALD). Despite the high sequence identity between trypanosomatid aldolases, the Nb474-based immunoassay is highly specific for T...
September 15, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28913971/chemical-functionalization-strategies-and-intracellular-applications-of-nanobodies
#2
Dominik Schumacher, Jonas Helma, Anselm F L Schneider, Heinrich Leonhardt, Christian Hackenberger
Nanobodies can be considered as next-generation life science tools for the recognition and modulation of antigens that are inaccessible to conventional antibodies. Due to their compact structure and high stability, nanobodies see frequent usage in basic research. Their chemical functionalization facilitates powerful diagnostic tools and opens the way towards promising therapeutic applications. In this review, central aspects of nanobody functionalization are given together with selected applications in molecular cell biology...
September 15, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28901744/time-resolved-fluorescence-immunochromatographic-assay-developed-using-two-idiotypic-nanobodies-for-rapid-quantitative-and-simultaneous-detection-of-aflatoxin-and-zearalenone-in-maize-and-its-products
#3
Xiaoqian Tang, Peiwu Li, Qi Zhang, Zhaowei Zhang, Wen Zhang, Jun Jiang
Aflatoxins and zearalenone (ZEN) are highly common mycotoxins in maize and maize-based products. This study aimed to report a time-resolved fluorescence immunochromatographic assay (TRFICA) developed using two idiotypic nanobodies for rapid, quantitative, and simultaneous detection of aflatoxin B1 (AFB1) and ZEN in maize and its products. A novel Eu/Tb(III) nanosphere with enhanced fluorescence was prepared as a label and conjugated to anti-idiotypic nanobody (AIdnb) and monoclonal antibody (mAb). On the basis of nanosphere-antibody conjugation, two patterns of competitive time-resolved strip methods (AIdnb-TRFICA and mAb-TRFICA) were established and compared...
September 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28901254/therapeutics-and-immunoprophylaxis-against-noroviruses-and-rotaviruses
#4
Souvik Ghosh, Yashpal Singh Malik, Nobumichi Kobayashi
Noroviruses and rotaviruses are important viral etiologies of severe gastroenteritis. Noroviruses are the primary cause of non-bacterial diarrheal outbreaks in humans, whilst rotaviruses are a major cause of childhood diarrhea. Although both enteric pathogens substantially impact human health and economies, there are no approved drugs against noroviruses and rotaviruses, so far. On the other hand, whilst the currently licensed rotavirus vaccines have been successfully implemented in over 100 countries, the most advanced norovirus vaccine has recently completed phase-I and II trials...
September 12, 2017: Current Drug Metabolism
https://www.readbyqxmd.com/read/28884572/nanobody-based-apolipoprotein-e-immunosensor-for-point-of-care-testing
#5
Xiang Ren, Junrong Yan, Dan Wu, Qin Wei, Yakun Wan
Alzheimer's disease (AD) biomarkers can reflect the neurochemical indicators used to estimate the risk in clinical nephrology. Apolipoprotein E (ApoE) is an early biomarker for AD in clinical diagnosis. In this research, through bactrian camel immunization, lymphocyte isolation, RNA extraction, and library construction, ApoE-specific Nbs with high affinity were successfully separated from an immune phage display nanobody library. Herein, a colorimetric immunosensor was developed for the point-of-care testing of ApoE by layer-by-layer nanoassembly techniques and novel nanobodies (Nbs)...
September 11, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28875290/evaluation-of-99m-tc-radiolabeled-macrophage-mannose-receptor-specific-nanobodies-for-targeting-of-atherosclerotic-lesions-in-mice
#6
Gezim Bala, Henri Baudhuin, Isabel Remory, Kris Gillis, Pieterjan Debie, Ahmet Krasniqi, Tony Lahoutte, Geert Raes, Nick Devoogdt, Bernard Cosyns, Sophie Hernot
PURPOSE: Macrophage accumulation characterizes the development of atherosclerotic plaques, and the presence of certain macrophage subsets might be an indicator of plaque phenotype and (in)stability. The macrophage mannose receptor (MMR) is expressed on alternatively activated macrophages and found at sites of intraplaque hemorrhage and neovascularization. It has been proposed as target to identify vulnerable plaques. Therefore, we aimed to assess the feasibility of using anti-MMR nanobodies (Nbs) as molecular tracers for nuclear imaging in an animal model of atherosclerosis...
September 5, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/28874561/localized-cd47-blockade-enhances-immunotherapy-for-murine-melanoma
#7
Jessica R Ingram, Olga S Blomberg, Jonathan T Sockolosky, Lestat Ali, Florian I Schmidt, Novalia Pishesha, Camilo Espinosa, Stephanie K Dougan, K Christopher Garcia, Hidde L Ploegh, Michael Dougan
CD47 is an antiphagocytic ligand broadly expressed on normal and malignant tissues that delivers an inhibitory signal through the receptor signal regulatory protein alpha (SIRPα). Inhibitors of the CD47-SIRPα interaction improve antitumor antibody responses by enhancing antibody-dependent cellular phagocytosis (ADCP) in xenograft models. Endogenous expression of CD47 on a variety of cell types, including erythrocytes, creates a formidable antigen sink that may limit the efficacy of CD47-targeting therapies...
September 5, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28871254/single-domain-antibodies-as-versatile-affinity-reagents-for-analytical-and-diagnostic-applications
#8
REVIEW
Gualberto Gonzalez-Sapienza, Martín A Rossotti, Sofía Tabares-da Rosa
With just three CDRs in their variable domains, the antigen-binding site of camelid heavy-chain-only antibodies (HcAbs) has a more limited structural diversity than that of conventional antibodies. Even so, this does not seem to limit their specificity and high affinity as HcAbs against a broad range of structurally diverse antigens have been reported. The recombinant form of their variable domain [nanobody (Nb)] has outstanding properties that make Nbs, not just an alternative option to conventional antibodies, but in many cases, these properties allow them to reach analytical or diagnostic performances that cannot be accomplished with conventional antibodies...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28871249/neutralization-of-human-interleukin-23-by-multivalent-nanobodies-explained-by-the-structure-of-cytokine-nanobody-complex
#9
Aline Desmyter, Silvia Spinelli, Carlo Boutton, Michael Saunders, Christophe Blachetot, Hans de Haard, Geertrui Denecker, Maarten Van Roy, Christian Cambillau, Heidi Rommelaere
The heterodimeric cytokine interleukin (IL) 23 comprises the IL12-shared p40 subunit and an IL23-specific subunit, p19. Together with IL12 and IL27, IL23 sits at the apex of the regulatory mechanisms shaping adaptive immune responses. IL23, together with IL17, plays an important role in the development of chronic inflammation and autoimmune inflammatory diseases. In this context, we generated monovalent antihuman IL23 variable heavy chain domain of llama heavy chain antibody (VHH) domains (Nanobodies(®)) with low nanomolar affinity for human interleukin (hIL) 23...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28869728/-application-of-nanobody-in-cancer-treatment
#10
Yue Wu, Xiujing Hao, Min Li
Camelidae can produce a unique antibody that lacks light chain called variable heavy chain domain, also known as nanobodies. This antibody contains only one variable region, with high affinity, high stability, strong tissue penetration, efficient expression. Besides, their toxicity and immunogenicity are both low to be used for both therapeutic and diagnostic applications, as well as research tools. In this review, we discuss how nanobody has been explored as therapeutics in oncology, and provide ideas for the further development of nanobody...
July 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28861827/analysis-of-nanobody-epitope-interactions-in-living-cells-via-quantitative-protein-transport-assays
#11
Simone Früholz, Peter Pimpl
Over the past few decades, quantitative protein transport analyses have been used to elucidate the sorting and transport of proteins in the endomembrane system of plants. Here, we have applied our knowledge about transport routes and the corresponding sorting signals to establish an in vivo system for testing specific interactions between soluble proteins.Here, we describe the use of quantitative protein transport assays in tobacco mesophyll protoplasts to test for interactions occurring between a GFP-binding nanobody and its GFP epitope...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28861826/in-vivo-interaction-studies-by-measuring-f%C3%A3-rster-resonance-energy-transfer-through-fluorescence-lifetime-imaging-microscopy-fret-flim
#12
Florian Fäßler, Peter Pimpl
Combinations of multiple fluorescent fusion proteins are commonly generated and used for colocalization studies in live cell imaging but also biochemical analysis of protein-protein interactions by co-immunoprecipitation in vitro. Advanced microscopy techniques like Förster resonance energy transfer through fluorescence lifetime imaging microscopy (FRET/FLIM) nowadays enable the combination of both approaches. This opens up the possibility to perform a location-specific protein-protein interaction analysis in vivo...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28853185/a-camelid-nanobody-against-egfr-was-easily-obtained-through-refolding-of-inclusion-body-expressed-in-escherichia-coli
#13
Li Xu, Xiaoyu Song, Lingyun Jia
Using anti-EGFR (epidermal growth factor receptor) nanobody is a good choice for diagnoses and therapeutics for high EGFR expression diseases. In the present study, the percentage composition of anti-EGFR nanobody attained 25% of the total cell protein expressed in Escherichia coli BL21 (DE3). However, almost all nanobodies were expressed as inclusion bodies. To acquire active nanobodies, a series of dilution refolding procedures were optimized after inclusion bodies were dissolved into 6 M urea and purified with immobilized metal affinity chromatography...
November 7, 2016: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28845963/quantum-dot-based-theranostic-micelles-conjugated-with-an-anti-egfr-nanobody-for-triple-negative-breast-cancer-therapy
#14
Yuyuan Wang, Yidan Wang, Guojun Chen, Yitong Li, Wei Xu, Shaoqin Gong
A quantum-dot (QD)-based micelle conjugated with an anti-epidermal growth factor receptor (EGFR) nanobody (Nb) and loaded with an anticancer drug, aminoflavone (AF), has been engineered for EGFR-overexpressing cancer theranostics. The near-infrared (NIR) fluorescence of the indium phosphate core/zinc sulfide shell QDs (InP/ZnS QDs) allowed for in vivo nanoparticle biodistribution studies. The anti-EGFR nanobody 7D12 conjugation improved the cellular uptake and cytotoxicity of the QD-based micelles in EGFR-overexpressing MDA-MB-468 triple-negative breast cancer (TNBC) cells...
September 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28841807/development-of-a-novel-nano-sized-anti-vegfa-nanobody-with-enhanced-physicochemical-and-pharmacokinetic-properties
#15
Farnaz Khodabakhsh, Dariush Norouzian, Behrouz Vaziri, Reza Ahangari Cohan, Soroush Sardari, Fereidoun Mahboudi, Mahdi Behdani, Kamran Mansouri, Ardavan Mehdizadeh
Since physiological and pathological processes occur at nano-environments, nanotechnology has considered as an efficient tool for designing of next generation specific biomolecules with enhanced pharmacodynamic and pharmacodynamic properties. In the current investigation, by control of the size and hydrodynamic volume at the nanoscale, for the first time, physicochemical and pharmacokinetic properties of an anti-VEGFA nanobody was remarkably improved by attachment of a Proline-Alanine-Serine (PAS) rich sequence...
August 25, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28827559/selection-of-nanobodies-with-broad-neutralizing-potential-against-primary-hiv-1-strains-using-soluble-subtype-c-gp140-envelope-trimers
#16
Kathrin Koch, Sarah Kalusche, Jonathan L Torres, Robyn L Stanfield, Welbeck Danquah, Kamal Khazanehdari, Hagen von Briesen, Eric R Geertsma, Ian A Wilson, Ulrich Wernery, Friedrich Koch-Nolte, Andrew B Ward, Ursula Dietrich
Broadly neutralizing antibodies (bnAbs) against HIV-1 protect from infection and reduce viral load upon therapeutic applications. However no vaccine was able so far to induce bnAbs demanding their expensive biotechnological production. For clinical applications, nanobodies (VHH) derived from heavy chain only antibodies from Camelidae, may be better suited due to their small size, high solubility/stability and extensive homology to human VH3 genes. Here we selected broadly neutralizing nanobodies by phage display after immunization of dromedaries with different soluble trimeric envelope proteins derived from HIV-1 subtype C...
August 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28815497/viral-delivery-of-gfp-dependent-recombinases-to-the-mouse-brain
#17
Jonathan C Y Tang, Stephanie Rudolph, Constance L Cepko
Many genetic tools have been developed that use green fluorescent protein (GFP) and its derivatives for labeling specific cell populations in organisms and in cell culture. To extend the use of GFP beyond labeling purposes, we developed methods and reagents that use GFP as a driver of biological activities. We used nanobodies that bind GFP to engineer CRE-DOG and Flp-DOG, recombinases that can induce Cre/lox and Flp/FRT recombination in a GFP-dependent manner, respectively. Here, we present a protocol to deliver CRE-DOG and Flp-DOG into the mouse brain by recombinant AAV infection...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28811312/myosin-ii-is-not-required-for-drosophila-tracheal-branch-elongation-and-cell-intercalation
#18
Amanda Ochoa-Espinosa, Stefan Harmansa, Emmanuel Caussinus, Markus Affolter
The Drosophila tracheal system consists of an interconnected network of monolayered epithelial tubes that ensures oxygen transport in the larval and adult body. During tracheal dorsal branch (DB) development, individual DBs elongate as a cluster of cells, led by tip cells at the front and trailing cells in the rear. Branch elongation is accompanied by extensive cell intercalation and cell lengthening of the trailing stalk cells. Although cell intercalation is governed by Myosin II (MyoII)-dependent forces during tissue elongation in the Drosophila embryo that lead to germ-band extension, it remained unclear whether MyoII plays a similar active role during tracheal branch elongation and intercalation...
August 15, 2017: Development
https://www.readbyqxmd.com/read/28796912/nanobody-mediated-resistance-to-grapevine-fanleaf-virus-in-plants
#19
Caroline Hemmer, Samia Djennane, Léa Ackerer, Kamal Hleibieh, Aurélie Marmonier, Sophie Gersch, Shahinez Garcia, Emmanuelle Vigne, Véronique Komar, Mireille Perrin, Claude Gertz, Lorène Belval, François Berthold, Baptiste Monsion, Corinne Schmitt-Keichinger, Olivier Lemaire, Bernard Lorber, Carlos Gutiérrez, Serge Muyldermans, Gérard Demangeat, Christophe Ritzenthaler
Since their discovery, single-domain antigen-binding fragments of camelid-derived heavy chain-only antibodies, also known as Nanobodies (Nbs), have proven to be of outstanding interest as therapeutics against human diseases and pathogens including viruses, but their use against phytopathogens remains limited. Many plant viruses including Grapevine fanleaf virus (GFLV), a nematode-transmitted icosahedral virus and causal agent of fanleaf degenerative disease, have worldwide distribution and huge burden on crop yields representing billions of US dollars of losses annually, yet solutions to combat these viruses are often limited or inefficient...
August 10, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28772027/escherichia-coli-surface-display-for-the-selection-of-nanobodies
#20
REVIEW
Valencio Salema, Luis Ángel Fernández
Nanobodies (Nbs) are the smallest functional antibody fragments known in nature and have multiple applications in biomedicine or environmental monitoring. Nbs are derived from the variable segment of camelid heavy chain-only antibodies, known as VHH. For selection, libraries of VHH gene segments from naïve, immunized animals or of synthetic origin have been traditionally cloned in E. coli phage display or yeast display systems, and clones binding the target antigen recovered, usually from plastic surfaces with the immobilized antigen (phage display) or using fluorescence-activated cell sorting (FACS; yeast display)...
August 3, 2017: Microbial Biotechnology
keyword
keyword
58440
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"