keyword
MENU ▼
Read by QxMD icon Read
search

nanobodies

keyword
https://www.readbyqxmd.com/read/28526745/identification-and-characterisation-of-nanobodies-targeting-the-epha4-receptor
#1
Lies Schoonaert, Laura Rué, Bart Roucourt, Mieke Timmers, Susan Little, Lucía Chávez Gutiérrez, Maarten Dewilde, Peter Joyce, Adam Curnock, Peter Weber, Jurgen Haustraete, Gholamreza Hassanzadeh-Ghassabeh, Bart De Strooper, Ludo Van Den Bosch, Philip Van Damme, Robin Lemmens, Wim Robberecht
The ephrin receptor A4 (EphA4) is one of the receptors in the ephrin system that plays a pivotal role in a variety of cell-cell interactions, mostly studied during development. In addition, EphA4 has been found to play a role in cancer biology as well as in the pathogenesis of several neurological disorders such as stroke, spinal cord injury, multiple sclerosis (MS), amyotrophic lateral sclerosis (ALS) and Alzheimer's disease (AD). Pharmacological blocking of EphA4 has been suggested to be a therapeutic strategy for these disorders...
May 19, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28507794/a-novel-nanobody-based-target-module-for-retargeting-of-t-lymphocytes-to-egfr-expressing-cancer-cells-via-the-modular-unicar-platform
#2
Susann Albert, Claudia Arndt, Anja Feldmann, Ralf Bergmann, Dominik Bachmann, Stefanie Koristka, Florian Ludwig, Pauline Ziller-Walter, Alexandra Kegler, Sebastian Gärtner, Marc Schmitz, Armin Ehninger, Marc Cartellieri, Gerhard Ehninger, Hans-Jürgen Pietzsch, Jens Pietzsch, Jörg Steinbach, Michael Bachmann
Recent treatments of leukemias with chimeric antigen receptor (CAR) expressing T cells underline their impressive therapeutic potential. However, once adoptively transferred into patients, there is little scope left to shut them down after elimination of tumor cells or in case adverse side effects occur. This becomes of special relevance if they are directed against commonly expressed tumor associated antigens (TAAs) such as receptors of the ErbB family. To overcome this limitation, we recently established a modular CAR platform technology termed UniCAR...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/28507719/broad-substrate-tolerance-of-tubulin-tyrosine-ligase-enables-one-step-site-specific-enzymatic-protein-labeling
#3
Dominik Schumacher, Oliver Lemke, Jonas Helma, Lena Gerszonowicz, Verena Waller, Tina Stoschek, Patrick M Durkin, Nediljko Budisa, Heinrich Leonhardt, Bettina G Keller, Christian P R Hackenberger
The broad substrate tolerance of tubulin tyrosine ligase is the basic rationale behind its wide applicability for chemoenzymatic protein functionalization. In this context, we report that the wild-type enzyme enables ligation of various unnatural amino acids that are substantially bigger than and structurally unrelated to the natural substrate, tyrosine, without the need for extensive protein engineering. This unusual substrate flexibility is due to the fact that the enzyme's catalytic pocket forms an extended cavity during ligation, as confirmed by docking experiments and all-atom molecular dynamics simulations...
May 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28502790/crystallographic-and-biochemical-characterization-of-the-dimeric-architecture-of-site-2-protease
#4
Magdalena Schacherl, Monika Gompert, Els Pardon, Tobias Lamkemeyer, Jan Steyaert, Ulrich Baumann
Regulated intramembrane proteolysis by members of the site-2 protease family (S2P) is an essential signal transduction mechanism conserved from bacteria to humans. There is some evidence that extra-membranous domains, like PDZ and CBS domains, regulate the proteolytic activity of S2Ps and that some members act as dimers. Here we report the crystal structure of the regulatory CBS domain pair of S2P from Archaeoglobus fulgidus, AfS2P, in the apo and nucleotide-bound form in complex with a specific Nanobody from llama...
May 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28502431/a-novel-photoelectrochemical-immunosensor-by-integration-of-nanobody-and-zno-nanorods-for-sensitive-detection-of-nucleoside-diphosphatase-kinase-a
#5
Anran Liu, Kaifei Yin, Li Mi, Mengyao Ma, Yuanjian Liu, Ying Li, Wei Wei, Yuanjian Zhang, Songqin Liu
Nucleoside diphosphatase kinase A (NDPK-A) is a metastasis-suppressor protein and a biomarker that act on a wide range cancer cells to inhibit the potential metastasis. Herein, we present a simple photoelectrochemical immunosensor based on ZnO nanorod arrays for the sensitive detection of NDPK-A. The ZnO nanorod arrays cosensitized with CdS nanoparticles and Mn(2+) displayed a high and stable photocurrent response under irradiation. After anti-NPDK-A nanobodies were immobilized to the ZnO nanorod arrays, the proposed immunosensor can be utilized for detecting NPDK-A by monitoring the changes in the photocurrent signals of the electrode resulting from immunoreaction...
June 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28498803/differentially-expressed-proteins-in-glioblastoma-multiforme-identified-with-a-nanobody-based-anti-proteome-approach-and-confirmed-by-oncofinder-as-possible-tumor-class-predictive-biomarker-candidates
#6
Ivana Jovčevska, Neja Zupanec, Žiga Urlep, Andrej Vranič, Boštjan Matos, Clara Limbaeck Stokin, Serge Muyldermans, Michael P Myers, Anton A Buzdin, Ivan Petrov, Radovan Komel
Glioblastoma multiforme is the most frequent primary malignancy of the central nervous system. Despite remarkable progress towards an understanding of tumor biology, there is no efficient treatment and patient outcome remains poor. Here, we present a unique anti-proteomic approach for selection of nanobodies specific for overexpressed glioblastoma proteins. A phage-displayed nanobody library was enriched in protein extracts from NCH644 and NCH421K glioblastoma cell lines. Differential ELISA screenings revealed seven nanobodies that target the following antigens: the ACTB/NUCL complex, VIM, NAP1L1, TUFM, DPYSL2, CRMP1, and ALYREF...
April 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/28494149/comparison-of-three-anti-hapten-vhh-selection-strategies-for-the-development-of-highly-sensitive-immunoassays-for-microcystins
#7
Macarena Pírez-Schirmer, Martin A Rossotti, Natalia Badagian, Carmen Leizagoyen, Beatriz Margarita Brena, Gualberto G Gonzalez-Sapienza
Owing to their reproducibility, stability and cost-effective production, the recombinant variable domains of heavy-chain-only antibodies (VHHs) are becoming a salient option as immunoassay reagents. Recently, there have been several reports describing their application to the detection of small molecules (haptens). However, lacking the heavy-light chain interface of conventional antibodies, VHHs are not particularly apt to bind small analytes and failures are not uncommon. Here we describe the construction of a VHH phage display library against the cyanobacterial hepatotoxin microcystin LR, and its selection using competitive panning and two novel panning strategies...
May 11, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28490657/targeting-endogenous-proteins-for-degradation-through-the-affinity-directed-protein-missile-system
#8
Luke J Fulcher, Luke D Hutchinson, Thomas J Macartney, Craig Turnbull, Gopal P Sapkota
Targeted proteolysis of endogenous proteins is desirable as a research toolkit and in therapeutics. CRISPR/Cas9-mediated gene knockouts are irreversible and often not feasible for many genes. Similarly, RNA interference approaches necessitate prolonged treatments, can lead to incomplete knockdowns and are often associated with off-target effects. Targeted proteolysis can overcome these limitations. In this report, we describe an affinity-directed protein missile (AdPROM) system that harbours the von Hippel-Lindau (VHL) protein, the substrate receptor of the Cullin2 (CUL2) E3 ligase complex, tethered to polypeptide binders that selectively bind and recruit endogenous target proteins to the CUL2-E3 ligase complex for ubiquitination and proteasomal degradation...
May 2017: Open Biology
https://www.readbyqxmd.com/read/28482214/active-state-structures-of-g-protein-coupled-receptors-highlight-the-similarities-and-differences-in-the-g-protein-and-arrestin-coupling-interfaces
#9
REVIEW
Byron Carpenter, Christopher G Tate
G protein-coupled receptors (GPCRs) regulate cellular signalling through heterotrimeric G proteins and arrestins in response to an array of extracellular stimuli. Structure determination of GPCRs in an active conformation bound to intracellular signalling proteins has proved to be highly challenging. Nonetheless, three new structures of GPCRs in an active state have been published during the last year, namely the adenosine A2A receptor (A2AR) bound to an engineered G protein, opsin bound to visual arrestin and the μ opioid receptor (μOR) bound to a G protein-mimicking nanobody...
May 5, 2017: Current Opinion in Structural Biology
https://www.readbyqxmd.com/read/28481446/how-different-are-g-protein-stabilized-agonist-gpcr-complexes-from-their-nanobody-stabilized-equivalents
#10
Noureldin Saleh, Passainte Ibrahim, Tim Clark
Protein nanobodies have been used successfully as surrogates for unstable G-proteins in order to crystallize G-protein coupled receptors (GPCRs) in their active states. We have now used molecular dynamics (MD) simulations including metadynamics enhanced sampling to investigate the similarities and differences between GPCR-agonist ternary complexes with the -subunits of the appropriate G-proteins and with the protein nanobodies (intracellular binding partners, IBPs) used for crystallization. In two of three receptors considered, the agonist binding mode differs significantly between the two alternative ternary complexes...
May 8, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28475474/antibodies-targeting-g-protein-coupled-receptors-recent-advances-and-therapeutic-challenges
#11
Mohammed Akli Ayoub, Pascale Crépieux, Markus Koglin, Marc Parmentier, Jean-Philippe Pin, Anne Poupon, Eric Reiter, Martine Smit, Jan Steyaert, Hervé Watier, Trevor Wilkinson
Le STUDIUM conference was held November 24-25, 2016 in Tours, France as a satellite workshop of the 5(th) meeting of the French GDR 3545 on "G Protein-Coupled Receptors (GPCRs) - From Physiology to Drugs", which was held in Tours during November 22-24, 2016. The conference gathered speakers from academia and industry considered to be world leaders in the molecular pharmacology and signaling of GPCRs, with a particular interest in the development of therapeutic GPCR antibodies (Abs). The main topics were new advances and challenges in the development of antibodies targeting GPCRs and their potential applications to the study of the structure and function of GPCRs, as well as their implication in physiology and pathophysiology...
May 5, 2017: MAbs
https://www.readbyqxmd.com/read/28474754/ultrasensitive-measurement-of-ca-2-influx-into-lipid-vesicles-induced-by-protein-aggregates
#12
Patrick Flagmeier, Suman De, David C Wirthensohn, Steven F Lee, Cécile Vincke, Serge Muyldermans, Tuomas P J Knowles, Sonia Gandhi, Christopher M Dobson, David Klenerman
To quantify and characterize the potentially toxic protein aggregates associated with neurodegenerative diseases, a high-throughput assay based on measuring the extent of aggregate-induced Ca(2+) entry into individual lipid vesicles has been developed. This approach was implemented by tethering vesicles containing a Ca(2+) sensitive fluorescent dye to a passivated surface and measuring changes in the fluorescence as a result of membrane disruption using total internal reflection microscopy. Picomolar concentrations of Aβ42 oligomers could be observed to induce Ca(2+) influx, which could be inhibited by the addition of a naturally occurring chaperone and a nanobody designed to bind to the Aβ peptide...
May 5, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28471361/camelid-nanobodies-used-as-crystallization-chaperones-for-different-constructs-of-porm-a-component-of-the-type-ix-secretion-system-from-porphyromonas-gingivalis
#13
Yoan Duhoo, Jennifer Roche, Thi Trang Nhung Trinh, Aline Desmyter, Anaïs Gaubert, Christine Kellenberger, Christian Cambillau, Alain Roussel, Philippe Leone
PorM is a membrane protein that is involved in the assembly of the type IX secretion system (T9SS) in Porphyromonas gingivalis, a major bacterial pathogen that is responsible for periodontal disease in humans. In the context of structural studies of PorM to better understand T9SS assembly, four camelid nanobodies were selected, produced and purified, and their specific interaction with the N-terminal or C-terminal part of the periplasmic domain of PorM was investigated. Diffracting crystals were also obtained, and the structures of the four nanobodies were solved by molecular replacement...
May 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28469136/identification-of-a-nanobody-specific-to-human-pulmonary-surfactant-protein-a
#14
Xian He, Shan-Mei Wang, Zhao Fang Yin, Meng-Meng Zhao, Nan Li, Feng Yu, Liu-Sheng Wang, Yang Hu, Yu-Kui Du, Shan-Shan Du, Yan Li, Ya-Ru Wei, Shan-Shan Chen, Jian-Hua He, Dong Weng, Hui-Ping Li
Nanobody (Nb) is a promising vector for targeted drug delivery. This study aims to identify an Nb that can specifically target the lung by binding human pulmonary surfactant protein A (SP-A). Human lung frozen tissue sections were used for 3 rounds of biospanning of our previously constructed Nb library for rat SP-A to establish a sub-library of Nb, which specifically bound human lung tissues. Phage-ELISA was performed to screen the sub-library to identify Nb4, which specifically bound human SP-A. The binding affinity Kd of Nb4 to recombinant human SP-A was 7...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28468279/applying-unconventional-secretion-in-ustilago-maydis-for-the-export-of-functional-nanobodies
#15
Marius Terfrüchte, Michèle Reindl, Silke Jankowski, Parveen Sarkari, Michael Feldbrügge, Kerstin Schipper
Exploiting secretory pathways for production of heterologous proteins is highly advantageous with respect to efficient downstream processing. In eukaryotic systems the vast majority of heterologous proteins for biotechnological application is exported via the canonical endoplasmic reticulum-Golgi pathway. In the endomembrane system target proteins are often glycosylated and may thus be modified with foreign glycan patterns. This can be destructive for their activity or cause immune reactions against therapeutic proteins...
April 29, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28461199/new-structural-formats-of-therapeutic-antibodies-for-rheumatology
#16
Christophe Dumet, Jérémy Pottier, Valérie Gouilleux, Hervé Watier
Pharmaceutical companies strive continuously to develop better medications in order to remain competitive. In the arena of monoclonal antibodies and related biologics (fusion proteins containing an IgG Fc fragment), the thrust is not only toward identifying new targets, but also toward developing new molecular formats. Here, new-generation antibodies used to treat rheumatic diseases are discussed, with emphasis on relations linking structure to pharmacological effects and on the improvements expected from the new formats...
April 28, 2017: Joint, Bone, Spine: Revue du Rhumatisme
https://www.readbyqxmd.com/read/28460522/nanobody-based-immunoassay-for-human-soluble-epoxide-hydrolase-detection-using-polymeric-horseradish-peroxidase-polyhrp-for-signal-enhancement-the-rediscovery-of-polyhrp
#17
Dongyang Li, Yongliang Cui, Christophe Morisseau, Shirley J Gee, Candace S Bever, Xiangjiang Liu, Jian Wu, Bruce D Hammock, Yibin Ying
Soluble epoxide hydrolase (sEH) is a potential pharmacological target for treating hypertension, vascular inflammation, cancer, pain, and multiple cardiovascular related diseases. A variable domain of the heavy chain antibody (termed single domain antibody (sdAb), nanobody, or VHH) possesses the advantages of small size, high stability, ease of genetic manipulation, and ability for continuous manufacture, making such nanobody a superior choice as an immunoreagent. In this work, we developed an ultrasensitive nanobody based immunoassay for human sEH detection using polymeric horseradish peroxidase (PolyHRP) for signal enhancement...
May 10, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28451377/dna-barcoded-labeling-probes-for-highly-multiplexed-exchange-paint-imaging
#18
Sarit S Agasti, Yu Wang, Florian Schueder, Aishwarya Sukumar, Ralf Jungmann, Peng Yin
Recent advances in super-resolution fluorescence imaging allow researchers to overcome the classical diffraction limit of light, and are already starting to make an impact in biology. However, a key challenge for traditional super-resolution methods is their limited multiplexing capability, which prevents a systematic understanding of multi-protein interactions on the nanoscale. Exchange-PAINT, a recently developed DNA-based multiplexing approach, in theory facilitates spectrally-unlimited multiplexing by sequentially imaging target molecules using orthogonal dye-labeled 'imager' strands...
April 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28449310/rational-design-of-nanobody80-loop-peptidomimetics-towards-biased-%C3%AE-2-adrenergic-receptor-ligands
#19
Charlotte Martin, Samuel Moors, Mia Danielsen, Cecilia Betti, Cecilia Fabris, Daniel Sejer Pedersen, Els Pardon, Marion Peyressatre, Krisztina Fehér, Jose C Martins, Jesper Mosolff Mathiesen, May Morris, Nick Devoogdt, Vicky Caveliers, Frank De Proft, Jan Steyaert, Steven Ballet
G protein-coupled receptors (GPCRs) play an important role for many cellular responses, and as such their mechanism of action is of utmost interest. To gain insight into the active conformation of GPCRs, the X-ray crystal structures of Nanobody(Nb)-stabilized β2-adrenergic receptor (β2AR) have been reported. Nb80 in particular is able to bind the intracellular G protein binding site of β2AR and stabilize the receptors in an active conformation. Within Nb80, the complementarity-determining region 3 (CDR3) is responsible for most of the binding interactions...
April 27, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28448738/site-specific-recruitment-of-epigenetic-factors-with-a-modular-crispr-cas-system
#20
Tobias Anton, Sebastian Bultmann
Dissecting the complex network of epigenetic modifications requires tools that combine precise recognition of DNA sequences with the capability to modify epigenetic marks. The CRISPR/Cas system has been proven to be a valuable addition to existing methodologies that fulfill these tasks. So far, sequence-specific editing of epigenetic modifications such as DNA methylation and histone posttranslational modifications relied on direct fusions of enzymatically inactivated Cas9 (dCas9) with epigenetic effectors. Here, we report a novel, modular system that facilitates the recruitment of any GFP-tagged protein to desired genomic loci...
February 23, 2017: Nucleus
keyword
keyword
58440
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"