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https://www.readbyqxmd.com/read/27910874/comprehensive-evaluation-of-molecular-enhancers-of-the-isothermal-exponential-amplification-reaction
#1
Ellie Mok, Eugene Wee, Yuling Wang, Matt Trau
The exponential amplification reaction (EXPAR) is an emerging isothermal nucleic acid amplification method with high potential for molecular diagnostics due to its isothermal nature and high amplification efficiency. However, the use of EXPAR is limited by the high levels of non-specific amplification. Hence, methods that can improve the specificity of EXPAR are desired to facilitate its widespread adoption in practice. Herein, we proposed a strategy to improve EXPAR performance by using molecular enhancers...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27909769/evaluation-of-131-i-scintigraphy-and-stimulated-thyroglobulin-levels-in-the-follow-up-of-patients-with-dtc-a-retrospective-analysis-of-1420-patients
#2
Jose Manuel Gonzalez Carvalho, Dennis Görlich, Otmar Schober, Christian Wenning, Burkhard Riemann, Frederik Anton Verburg, Alexis Vrachimis
AIM: To study the clinical yield of diagnostic whole body (131)I scintigraphy (DxWBS) in the follow-up of differentiated thyroid carcinoma (DTC) patients in relation to stimulated thyroglobulin (sTg) in the initial post-ablation setting, as well as in the setting of repeated monitoring in course of further DTC follow-up. METHODS: Data of 1420 thyroidectomized and radioiodine remnant-ablated DTC patients following a well-defined therapy and standardized follow-up protocol were evaluated...
December 1, 2016: European Journal of Nuclear Medicine and Molecular Imaging
https://www.readbyqxmd.com/read/27902900/sensitivity-and-specificity-estimation-for-the-clinical-diagnosis-of-highly-pathogenic-avian-influenza-in-the-egyptian-participatory-disease-surveillance-program
#3
C Verdugo, I El Masry, Y Makonnen, H Hannah, F Unger, M Soliman, S Galal, J Lubroth, D Grace
Many developing countries lack sufficient resources to conduct animal disease surveillance. In recent years, participatory epidemiology has been used to increase the cover and decrease the costs of surveillance. However, few diagnostic performance assessments have been carried out on participatory methods. The objective of the present study was to estimate the diagnostic performance of practitioners working for the Community-Based Animal Health and Outreach (CAHO) program, which is a participatory disease surveillance system for the detection of highly pathogenic avian influenza outbreaks in Egypt...
December 2016: Avian Diseases
https://www.readbyqxmd.com/read/27901394/computed-tomographic-anatomy-of-the-heads-of-blue-and-gold-macaws-ara-ararauna-african-grey-parrots-psittacus-erithacus-and-monk-parakeets-myiopsitta-monachus
#4
Irene A Veladiano, Tommaso Banzato, Luca Bellini, Alessandro Montani, Salvatore Catania, Alessandro Zotti
OBJECTIVE To create an atlas of the normal CT anatomy of the head of blue-and-gold macaws (Ara ararauna), African grey parrots (Psittacus erithacus), and monk parakeets (Myiopsitta monachus). ANIMALS 3 blue-and-gold macaws, 5 African grey parrots, and 6 monk parakeets and cadavers of 4 adult blue-and-gold macaws, 4 adult African grey parrots, and 7 monk parakeets. PROCEDURES Contrast-enhanced CT imaging of the head of the live birds was performed with a 4-multidetector-row CT scanner. Cadaveric specimens were stored at -20°C until completely frozen, and each head was then sliced at 5-mm intervals to create reference cross sections...
December 2016: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/27899974/validation-of-the-diagnostic-utility-of-urinary-midkine-for-the-detection-of-bladder-cancer
#5
Dana Vu Van, Ulrike Heberling, Manfred P Wirth, Susanne Fuessel
As it has been demonstrated previously that midkine (also known as neurite growth-promoting factor 2) protein levels in urine of bladder cancer (BCa) patients are increased compared to healthy controls, the present study validated the diagnostic utility of midkine in an independent patient cohort and compared the observed values with voided urine cytology (VUC), which is the current reference standard for non-invasive diagnosis of BCa. Voided urine samples were prospectively collected from 92 BCa patients and 70 control subjects...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27898397/multicenter-performance-evaluation-of-a-second-generation-cortisol-assay
#6
Michael Vogeser, Jürgen Kratzsch, Yoon Ju Bae, Mathias Bruegel, Uta Ceglarek, Tom Fiers, Alexander Gaudl, Hedwig Kurka, Christoph Milczynski, Cristina Prat Knoll, Anna C Suhr, Daniel Teupser, Ingrid Zahn, Richard E Ostlund
BACKGROUND: Untreated disorders of the adrenocortical system, such as Cushing's or Addison's disease, can be fatal, and accurate quantification of a patient's cortisol levels is vital for diagnosis. The objective of this study was to assess the analytical performance of a new fully-automated Elecsys® Cortisol II assay (second generation) to measure cortisol levels in serum and saliva. METHODS: Four European investigational sites assessed the intermediate precision and reproducibility of the Cortisol II assay (Roche Diagnostics) under routine conditions...
November 29, 2016: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/27893962/proteopathic-strains-and-the-heterogeneity-of-neurodegenerative-diseases
#7
Lary C Walker
Most age-related neurodegenerative diseases are associated with the misfolding and aberrant accumulation of specific proteins in the nervous system. The proteins self-assemble and spread by a prion-like process of corruptive molecular templating, whereby abnormally folded proteins induce the misfolding and aggregation of like proteins into characteristic lesions. Despite the apparent simplicity of this process at the molecular level, diseases such as Alzheimer's, Parkinson's, Creutzfeldt-Jakob, and others display remarkable phenotypic heterogeneity, both clinically and pathologically...
November 23, 2016: Annual Review of Genetics
https://www.readbyqxmd.com/read/27893691/valuable-hematological-indicators-for-the-diagnosis-and-severity-assessment-of-chinese-children-with-community-acquired-pneumonia-prealbumin
#8
Jingjing Ning, Xiaonan Shao, Yibo Ma, Darong Lv
Chest X-ray is a "golden standard" for the diagnosis and severity assessment of community-acquired pneumonia (CAP). However, it cannot be used as routine examination of CAP in children. The present study aims to investigate the roles of prealbumin (PA) in CAP in children and further determine the usefulness of PA in diagnosis and severity assessment of CAP in children.This was a retrospective analysis of 174 cases of hospitalized children with CAP. The following indicators were recorded: vital sign, inflammatory indexes, PA, and respiratory pathogens immunoglobulin M antibody test results...
November 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27893664/genetic-aspects-of-pituitary-carcinoma-a-systematic-review
#9
Zijiang Yang, Ting Zhang, Heng Gao
BACKGROUND: Pituitary carcinoma (PC) is a rare type of malignant intracranial neoplasm defined as distant metastasis of pituitary adenoma (PA). Although PC incidence is low because only 0.1% to 0.2% of PAs ultimately develop into PCs, the prognosis is poor and 66% of patients die within the first year. Existing therapeutic measures, including surgical removal, chemotherapy, and radiotherapy, have limited effectiveness. The lack of efficacy of current treatments is largely caused by the limited understanding of the molecular pathogenesis of PA and the malignant transformation to PC...
November 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27892880/mri-cross-sectional-atlas-of-normal-canine-cervical-musculoskeletal-structure
#10
M Alizadeh, C Zindl, M J Allen, G G Knapik, N Fitzpatrick, W S Marras
Although magnetic resonance imaging (MRI) has been increasingly used as a diagnostic tool for cervical spine injuries in canines, a comprehensive normal MRI anatomy of the canine cervical spine muscles is lacking. Therefore, the purpose of this study was to build a magnetic resonance imaging atlas of the normal cross sectional anatomy of the muscles of the canine cervical spine. MRI scans were performed on a canine cadaver using a combination of T1 and T2-weighted images in the transverse, sagittal and dorsal planes acquired at a slice thickness of 1mm...
September 30, 2016: Research in Veterinary Science
https://www.readbyqxmd.com/read/27890084/comparison-of-molecular-and-conventional-methods-for-the-diagnosis-of-fasciola-hepatica-infection-in-the-field
#11
Maria Immaculata Arifin, Johan Höglund, Adam Novobilský
The liver fluke, Fasciola hepatica, is one of the major parasite threats to livestock industries world-wide. In sheep and cattle, F. hepatica infection is commonly diagnosed using a range of methods. Aside from conventional coprological and serological diagnostic methods, there are also several molecular methods available based on the detection of liver fluke DNA in faeces. In this study, the outcomes of faecal egg count (FEC), serology and coproantigen ELISA (cELISA) were compared with the performance of polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) in diagnosis of F...
December 15, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27886991/radiation-dose-for-pediatric-and-young-adult-ct-a-survey-to-establish-age-based-reference-levels-of-2015-2016-in-korea
#12
MoonChan Kim, KwangHyun Chang, JeongHoon Hwang, YoonChul Nam, DongKyoon Han, Joon Yoon
To assess the doses delivered to pediatric patients during computed tomography (CT) examinations of the brain, chest, high-resolution lung and abdomen, and to establish diagnostic reference levels (DRLs) for various age groups in Korea. Dose survey was done to the 19 hospitals performing CT on children, addressing the scan parameters, volume CT dose index (CTDIvol) and dose length product (DLP). Per five age group (0, 1, 2-5, 6-10, 11-17 y of age), the proposed DRLs for brain, chest, high-resolution lung and abdomen CT are, respectively, in terms of CTDIvol: 18, 23, 26, 31, 36 mGy; 2, 3, 4, 6, 8 mGy; 2, 3, 4, 5, 7 mGy; 3, 4, 5, 6, 9 mGy; and in terms of DLP: 260, 350, 420, 500, 620 mGy•cm; 50, 80, 100, 170, 340 mGy•cm; 30, 40, 60, 90, 280 mGy•cm; 70, 80, 200, 300, 500 mGy•cm...
November 24, 2016: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/27885225/the-physical-signs-of-impending-dehydration-among-elderly-people-in-nursing-homes-the-association-with-axillary-skin-temperature-humidity-intraoral-moisture-content-and-salivary-components
#13
Mayumi Okuyama, Masumi Nishida
PURPOSE: The aim of the present study was to examine the association between impending dehydration among elderly people in nursing homes and physical signs, including the axillary skin temperature, humidity, intraoral moisture content, and salivary components. METHODS: The study included 78 elderly individuals who required long-term care in a nursing home (11 men and 67 women; average age, 86.6±7.3 years). The elderly subjects were classified in two groups according to their serum osmolality levels: those with levels between the upper limit reference value (292 mOsm/kg H2O) and the diagnostic reference value of dehydration (300 mOsm/kg H2O) were classified into the boundary zone group and those with levels of <292 mOsm/kg H2O were classified into the normal range group...
2016: Nihon Ronen Igakkai Zasshi. Japanese Journal of Geriatrics
https://www.readbyqxmd.com/read/27885093/proposals-for-the-establishment-of-national-diagnostic-reference-levels-for-radiography-for-adult-patients-based-on-regional-dose-surveys-in-russian-federation
#14
A V Vodovatov, M I Balonov, V Yu Golikov, I G Shatsky, L A Chipiga, C Bernhardsson
In 2009-2014, dose surveys aimed to collect adult patient data and parameters of most common radiographic examinations were performed in six Russian regions. Typical patient doses were estimated for the selected examinations both in entrance surface dose and in effective dose. 75%-percentiles of typical patient effective dose distributions were proposed as preliminary regional diagnostic reference levels (DRLs) for radiography. Differences between the 75%-percentiles of regional typical patient dose distributions did not exceed 30-50% for the examinations with standardized clinical protocols (skull, chest and thoracic spine) and a factor of 1...
November 24, 2016: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/27882399/-circadian-rhythm-influence-on-epworth-sleepiness-scale-score
#15
M Herzog, A Bedorf, C Rohrmeier, T Kühnel, B Herzog, T Bremert, S Plontke, S Plößl
BACKGROUND: The Epworth Sleepiness Scale (ESS) is frequently used to determine daytime sleepiness in patients with sleep-disordered breathing. It is still unclear whether different levels of alertness induced by the circadian rhythm influence ESS score. OBJECTIVE: The aim of this study is to investigate the influence of circadian rhythm-dependent alertness on ESS performance. MATERIALS AND METHODS: In a monocentric prospective noninterventional observation study, 97 patients with suspected sleep-disordered breathing were investigated with respect to daytime sleepiness in temporal relationship to polysomnographic examination and treatment...
November 23, 2016: HNO
https://www.readbyqxmd.com/read/27881416/metagenomics-of-two-severe-foodborne-outbreaks-provides-diagnostic-signatures-and-signs-of-co-infection-not-attainable-by-traditional-methods
#16
Andrew D Huang, Chengwei Luo, Angela Pena-Gonzalez, Michael R Weigand, Cheryl Tarr, Konstantinos T Konstantinidis
: Diagnostic testing for foodborne pathogens relies on culture-based techniques that are not rapid enough for real-time disease surveillance and do not give a quantitative picture of pathogen abundance or the response of the natural microbiome. Powerful sequence-based, culture-independent approaches such as shotgun metagenomics could sidestep these limitations, and potentially reveal a pathogen-specific signature on the microbiome that would have implications not only for diagnostics but also for better understanding disease progression and pathogen ecology...
November 23, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27878805/pancreatic-enzyme-replacement-therapy-for-people-with-cystic-fibrosis
#17
REVIEW
Usha Rani Somaraju, Arturo Solis-Moya
BACKGROUND: Most people with cystic fibrosis (80% to 90%) need pancreatic enzyme replacement therapy to prevent malnutrition. Enzyme preparations need to be taken whenever food is taken, and the dose needs to be adjusted according to the food consumed. A systematic review on the efficacy and safety of pancreatic enzyme replacement therapy is needed to guide clinical practice, as there is variability between centres with respect to assessment of pancreatic function, time of commencing treatment, dose and choice of supplements...
November 23, 2016: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/27878270/alteration-of-histone-h3-lysine-9-dimethylation-in-peripheral-white-blood-cells-of-septic-patients-with-trauma-and-cancer
#18
Linghui Jiang, Yiqin Wang, Duming Zhu, Zhanggang Xue, Hailei Mao
The present study aimed to investigate the clinical significance of histone methylation in sepsis. A total of 43 blood samples from trauma and esophageal cancer patients with or without sepsis were collected. Immunofluorescence staining of isolated peripheral white blood cells (WBCs) was conducted. Co‑stained 293T cells served as a reference, to allow the levels of histone methylation in different types of WBCs from patients to be determined. Immunostaining analyses revealed different levels of histone 3 lysine 9 dimethylation (H3K9me2) in neutrophils (Neu), lymphocytes (Lym), and monocytes (Mon) from trauma patients...
November 22, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27876645/diagnostic-accuracy-of-rklo8-versus-rk26-elisas-for-screening-of-canine-visceral-leishmaniasis
#19
Lily P Martínez Abad, Caroline S Almeida, Ana Márcia M Mattos, Ana Carolina P Mendonça, Márcio J M Alves, Aimara C Pinheiro, Renato Porrozzi, Elfadil Abass, Ulrich Steinhoff, Henrique C Teixeira
Canine visceral leishmaniasis (CVL) represents an important public health issue. Despite numerous diagnostic tests available, CVL diagnosis still needs to be improved to achieve a more accurate detection rate. Recently, rKLO8, a new antigenic protein of Sudanese Leishmania donovani, was studied for the first time in diagnosis of human visceral leishmaniasis (HVL) and showed good performance. The present study aimed to evaluate serum reactivity to rKL08 and the reference antigen rK26, and to compare both diagnostic proteins with the combined DPP(®) CVL rapid test and ELISA (EIE-Bio-Manguinhos) confirmatory test, which are both recommended for the diagnosis of CVL in Brazil...
November 19, 2016: Acta Tropica
https://www.readbyqxmd.com/read/27876272/evaluation-of-rib-fractures-on-a-single-in-plane-image-reformation-of-the-rib-cage-in-ct-examinations
#20
Peter Dankerl, Hannes Seuss, Stephan Ellmann, Alexander Cavallaro, Michael Uder, Matthias Hammon
RATIONALE AND OBJECTIVES: This study aimed to evaluate the diagnostic performance of using a reformatted single-in-plane image reformation of the rib cage for the detection of rib fractures in computed tomography (CT) examinations, employing different levels of radiological experience. MATERIALS AND METHODS: We retrospectively evaluated 10 consecutive patients with and 10 patients without rib fractures, whose CT scans were reformatted to a single-in-plane image reformation of the rib cage...
November 18, 2016: Academic Radiology
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