Lena Hess, Verena Moos, Arnel A Lauber, Wolfgang Reiter, Michael Schuster, Natascha Hartl, Daniel Lackner, Thorina Boenke, Anna Koren, Paloma M Guzzardo, Brigitte Gundacker, Anna Riegler, Petra Vician, Claudia Miccolo, Susanna Leiter, Mahesh B Chandrasekharan, Terezia Vcelkova, Andrea Tanzer, Jun Qi Jun, James Bradner, Gerald Brosch, Markus Hartl, Christoph Bock, Tilmann Bürckstümmer, Stefan Kubicek, Susanna Chiocca, Srividya Bhaskara, Christian Seiser
The class I histone deacetylases are essential regulators of cell fate decisions in health and disease. While pan- and class-specific HDAC inhibitors are available, these drugs do not allow a comprehensive understanding of individual HDAC function, or the therapeutic potential of isoform-specific targeting. To systematically compare the impact of individual catalytic functions of HDAC1, HDAC2 and HDAC3, we generated human HAP1 cell lines expressing catalytically inactive HDAC enzymes. Using this genetic toolbox we compare the effect of individual HDAC inhibition with the effects of class I specific inhibitors on cell viability, protein acetylation and gene expression...
August 22, 2022: PLoS Genetics