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https://www.readbyqxmd.com/read/29045810/a-novel-next-generation-sequencing-assay-as-an-alternative-to-currently-available-methods-for-hepatitis-c-virus-genotyping
#1
G Dirani, E Paesini, E Mascetra, P Farabegoli, B Dalmo, B Bartolini, A R Garbuglia, M R Capobianchi, V Samri
Chronic HCV infection is one of the leading causes of liver-related death and in many countries it is a primary reason for having a liver transplant. HCV genotype identification has long been used in the clinical practice, since different genotypes have different response rates and required different doses and durations of IFN/RBV treatment; moreover both the frequency and the pattern of resistance to different Direct-Acting Antivirals (DAAs) classes are subtype specific. Hence the necessity to make an accurate HCV subtyping becomes a fundamental tool to optimize current and future clinical management of HCV infected subjects...
October 15, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/29041190/differential-optical-shadow-sensor-for-sub-nanometer-displacement-measurement-and-its-application-to-drag-free-satellites
#2
Andreas Zoellner, Si Tan, Shailendhar Saraf, Abdul Alfauwaz, Dan DeBra, Sasha Buchman, John A Lipa
We present a method for 3D sub-nanometer displacement measurement using a set of differential optical shadow sensors. It is based on using pairs of collimated beams on opposite sides of an object that are partially blocked by it. Applied to a sphere, our 3-axis sensor module consists of 8 parallel beam-detector sets for redundancy. The sphere blocks half of each beam's power in the nominal centered position, and any displacement can be measured by the differential optical power changes amongst the pairs of detectors...
October 16, 2017: Optics Express
https://www.readbyqxmd.com/read/29026820/a-qualitative-assessment-of-the-journey-to-delayed-breast-reconstruction
#3
Ho Man Cheng, Catherine McMillan, Joan E Lipa, Laura Snell
BACKGROUND: Canada has low immediate breast reconstruction (IBR) rates compared to the United States and Europe. Breast cancer survivors live with mastectomy defects sometimes for years, and this represents an area for improvement in cancer care. PURPOSE: This study qualitatively assessed (1) information provided about breast reconstruction at the time of cancer diagnosis among women seeking delayed breast reconstruction (DBR) and (2) referral practices for plastic surgery consultation for DBR...
August 2017: Plastic Surgery
https://www.readbyqxmd.com/read/29024035/the-role-of-human-papillomavirus-in-p16-positive-oral-cancers
#4
Simone Belobrov, Alyssa M Cornall, Richard J Young, Kendrick Koo, Christopher Angel, David Wiesenfeld, Danny Rischin, Suzanne M Garland, Michael McCullough
BACKGROUND: The aim of the present study was to identify the presence and frequency of human papillomavirus (HPV) nucleic acid in p16 positive oral squamous cell carcinomas (OSCC), to assess if the virus were transcriptionally active, and to assess the utility of p16 overexpression as a surrogate marker for HPV in OSCC. METHODS: Forty-six OSCC patients treated between 2007 and 2011 with available formalin-fixed paraffin embedded (FFPE) specimens were included. Twenty-three patients were positive for p16 by immunohistochemistry (IHC) and these were matched with 23 patients with p16 negative tumours...
October 11, 2017: Journal of Oral Pathology & Medicine
https://www.readbyqxmd.com/read/28963005/production-enhancement-of-the-extracellular-lipase-lipa-in-bacillus-subtilis-effects-of-regulatory-elements-and-sec-pathway-components
#5
Ran Jing Ma, Yan Hong Wang, Lu Liu, Lei Lei Bai, Rui Ban
Lipases are among the most versatile biocatalysts, and are used in a range of industrially relevant bioconversion reactions. However, the production of LipA in recombinant Bacillus subtilis is still limited, due to unresolved issues surrounding the regulation of the expression and secretion systems. In this study, the gene encoding LipA from B. subtilis 168 was expressed in BNA under the control of the P43 and the PAE promoter. The extracellular lipase activity of the resulting strains BNACL and BNAAL was 7...
September 26, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28958330/genetically-confirmed-familial-hypercholesterolemia-in-patients-with-acute-coronary-syndrome
#6
Almudena Amor-Salamanca, Sergio Castillo, Emiliano Gonzalez-Vioque, Fernando Dominguez, Lucía Quintana, Carla Lluís-Ganella, Juan Manuel Escudier, Javier Ortega, Enrique Lara-Pezzi, Luis Alonso-Pulpon, Pablo Garcia-Pavia
BACKGROUND: Genetic screening programs in unselected individuals with increased levels of low-density lipoprotein cholesterol (LDL-C) have shown modest results in identifying individuals with familial hypercholesterolemia (FH). OBJECTIVES: This study assessed the prevalence of genetically confirmed FH in patients with acute coronary syndrome (ACS) and compared the diagnostic performance of FH clinical criteria versus FH genetic testing. METHODS: Genetic study of 7 genes (LDLR, APOB, PCSK9, APOE, STAP1, LDLRAP1, and LIPA) associated with FH and 12 common alleles associated with polygenic hypercholesterolemia was performed in 103 patients with ACS, age ≤65 years, and LDL-C levels ≥160 mg/dl...
October 3, 2017: Journal of the American College of Cardiology
https://www.readbyqxmd.com/read/28954299/durability-of-protection-afforded-by-fewer-doses-of-the-hpv16-18-vaccine-the-cvt-trial
#7
RANDOMIZED CONTROLLED TRIAL
Mahboobeh Safaeian, Joshua N Sampson, Yuanji Pan, Carolina Porras, Troy J Kemp, Rolando Herrero, Wim Quint, Leen Jan van Doorn, John Schussler, Douglas R Lowy, John Schiller, Mark T Schiffman, Ana Cecilia Rodriguez, Mitchell H Gail, Allan Hildesheim, Paula Gonzalez, Ligia A Pinto, Aimée R Kreimer
Background: Previously, we demonstrated similar human papillomavirus (HPV)16/18 vaccine efficacy estimates and stable HPV16/18 antibody levels four years postvaccination in a nonrandomized analysis of women who received a varying number of doses of the bivalent HPV16/18 vaccine. Here we extend data to seven years following initial vaccination. Methods: We evaluated HPV16/18-vaccinated women who received one (n = 134), two (n 0/1 = 193, n 0/6 = 79), or three doses (n = 2043) to a median of 6...
February 1, 2018: Journal of the National Cancer Institute
https://www.readbyqxmd.com/read/28952562/human-papillomavirus-investigation-in-head-and-neck-squamous-cell-carcinoma-initial-report-from-the-low-risk-hpv-types-associations
#8
Mohammad Hadi Karbalaie Niya, Fahimeh Safarnezhad Tameshkel, Mahshid Panahi, Farah Bokharaei Salim, Seyed Hamid Reza Monavari, Hossein Keyvani
Background: Head and neck squamous cell carcinomas (HNSCC) are a major health issue in many parts of the world. Recently, attention has focused on the human papilloma virus (HPV) as a potential causative agent for HNSCC. This study aimed to survey HPV occurrence in HNSCCs as part of a comprehensive molecular epidemiology approach. Methods: In this retrospective study, patients were recruited from hospitals affiliated to the Iran University of Medical Sciences, Tehran, Iran. Formalin-fixed paraffin-embedded (FFPE) blocks were subjected to DNA isolation by QIAamp® DNA FFPE Tissue Kit and nested PCR, HPV-16 specific conventional PCR, and extra INNO-LiPA HPV genotyping assays were subsequently performed...
September 27, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28946879/contribution-of-single-amino-acid-and-codon-substitutions-to-the-production-and-secretion-of-a-lipase-by-bacillus-subtilis
#9
Pia Skoczinski, Kristina Volkenborn, Alexander Fulton, Anuseema Bhadauriya, Christina Nutschel, Holger Gohlke, Andreas Knapp, Karl-Erich Jaeger
BACKGROUND: Bacillus subtilis produces and secretes proteins in amounts of up to 20 g/l under optimal conditions. However, protein production can be challenging if transcription and cotranslational secretion are negatively affected, or the target protein is degraded by extracellular proteases. This study aims at elucidating the influence of a target protein on its own production by a systematic mutational analysis of the homologous B. subtilis model protein lipase A (LipA). We have covered the full natural diversity of single amino acid substitutions at 155 positions of LipA by site saturation mutagenesis excluding only highly conserved residues and qualitatively and quantitatively screened about 30,000 clones for extracellular LipA production...
September 25, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28930366/an-experimental-administration-of-pravastatin-in-patient-with-previous-multiple-pregnancy-losses
#10
Szymon Kozłowski, Michał Lipa, Jacek Lipa, Dorota Bomba-Opoń
No abstract text is available yet for this article.
2017: Ginekologia Polska
https://www.readbyqxmd.com/read/28922321/-patient-reported-outcomes-following-breast-conservation-therapy-and-barriers-to-referral-for-partial-breast-reconstruction
#11
Sebastian Q Vrouwe, Ron B Somogyi, Laura Snell, Catherine McMillan, Danny Vesprini, Joan E Lipa
BACKGROUND: The purpose of this study was to evaluate the self-reported aesthetic outcome of breast conservation therapy (BCT) in a generalized sample of patients, and to describe potential barriers to referral for partial breast reconstruction. METHODS: Consecutive BCT patients completing radiotherapy over a one-year period at a regional cancer centre were identified. Eligible patients were contacted via mail/email and invited to participate. Participants completed the BREAST-Q BCT module along with a questionnaire examining feelings about breast reconstruction...
September 14, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28884505/alternative-oils-tested-as-feedstocks-for-enzymatic-fames-synthesis-toward-a-more-sustainable-process
#12
Belén Infanzón, Silvia Cesarini, Josefina Martínez, F I Javier Pastor, Pilar Diaz
Previously isolated and characterized Pseudomonas lipases were immobilized in a low-cost MP-1000 support by a re-loading procedure that allowed a high activity per weight of support. Immobilized LipA, LipC, and LipCmut lipases, and commercial Novozym® 435 were tested for fatty acid methyl ester (FAMEs) synthesis using conventional and alternative feedstocks. Triolein and degummed soybean oils were used as model substrates, whereas waste cooking oil and M. circinelloides oil were assayed as alternative, low cost feedstocks, whose free fatty acid (FFA), and acylglyceride profile was characterized...
September 7, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28882870/crispr-cas9-mediated-gene-editing-in-human-ipsc-derived-macrophage-reveals-lysosomal-acid-lipase-function-in-human-macrophages
#13
Hanrui Zhang, Jianting Shi, Melanie A Hachet, Chenyi Xue, Robert C Bauer, Hongfeng Jiang, Wenjun Li, Junichiro Tohyama, John Millar, Jeffrey Billheimer, Michael C Phillips, Babak Razani, Daniel J Rader, Muredach P Reilly
OBJECTIVE: To gain mechanistic insights into the role of LIPA(lipase A), the gene encoding LAL (lysosomal acid lipase) protein, in human macrophages. APPROACH AND RESULTS: We used CRISPR (clustered regularly interspaced short palindromic repeats )/Cas9 (CRISPR-associated protein 9) technology to knock out LIPA in human induced pluripotent stem cells and then differentiate to macrophage (human-induced pluripotent stem cells-derived macrophage [IPSDM]) to explore the human macrophage LIPA loss-of-function phenotypes...
September 7, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28881270/molecular-and-clinical-characterization-of-a-series-of-patients-with-childhood-onset-lysosomal-acid-lipase-deficiency-retrospective-investigations-follow-up-and-detection-of-two-novel-lipa-pathogenic-variants
#14
Livia Pisciotta, Giulia Tozzi, Lorena Travaglini, Roberta Taurisano, Tiziano Lucchi, Giuseppe Indolfi, Francesco Papadia, Maja Di Rocco, Lorenzo D'Antiga, Patricia Crock, Komal Vora, Scott Nightingale, Helen Michelakakis, Anastasia Garoufi, Lilia Lykopoulou, Stefano Bertolini, Sebastiano Calandra
BACKGROUND AND AIMS: Childhood/Adult-onset Lysosomal Acid Lipase Deficiency (LAL-D) is a recessive disorder due to loss of function variants of LAL, the enzyme which hydrolyses cholesteryl esters, derived from internalized apoB containing lipoproteins. The disease is characterized by multi-organ involvement including the liver, spleen, intestine and cardiovascular system. The aim of this study was the clinical and molecular characterization of 14 (13 unrelated) previously unreported patients with childhood-onset LAL-D...
October 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28871123/the-role-of-hfq-in-regulation-of-lipa-expression-in-pseudomonas-protegens-pf-5
#15
Wu Liu, Menggang Li, Jinyong Yan, Yunjun Yan
Pseudomonas lipase is a well-studied lipase. However, few studies have been conducted to examine the mechanisms underlying the regulation of the lipase expression. Hfq is a global regulatory protein that, among others, controls the expression of multiple genes, regulate bacterial peristalsis, and participates in the regulation of quorum-sensing (QS) system. In this study, the effects of Hfq on lipase expression were investigated by knocking out the hfq and rsmY genes or overexpressing of hfq and rsmY genes...
September 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28860124/quantitative-analysis-of-the-proteome-response-to-the-histone-deacetylase-inhibitor-hdaci-vorinostat-in-niemann-pick-type-c1-disease
#16
Kanagaraj Subramanian, Navin Rauniyar, Mathieu Lavalleé-Adam, John R Yates, William E Balch
Niemann-Pick type C (NPC) disease is an inherited, progressive neurodegenerative disorder principally caused by mutations in the NPC1 gene. NPC disease is characterized by the accumulation of unesterified cholesterol in the late endosomes (LE) and lysosomes (LE) (LE/Ly). Vorinostat, a histone deacetylase inhibitor (HDACi), restores cholesterol homeostasis in fibroblasts derived from NPC patients; however, the exact mechanism by which Vorinostat restores cholesterol level is not known yet. In this study, we performed comparative proteomic profiling of the response of NPC1I1061T fibroblasts to Vorinostat...
August 31, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28851606/clinical-evaluation-of-a-newly-developed-automated-massively-parallel-sequencing-assay-for-hepatitis-c-virus-genotyping-and-detection-of-resistance-association-variants-comparison-with-a-line-probe-assay
#17
Narathon Manee, Nipa Thongbaiphet, Ekawat Pasomsub, Wasun Chantratita
Hepatitis C virus (HCV) infection is a leading cause of chronic liver disease, cirrhosis and hepatocellular carcinoma. Recently, HCV was classified into 6 major genotypes (GTs) and 67 subtypes (STs). Efficient genotyping has become an essential tool for prognosis and indicating suitable treatment, prior to starting therapy in all HCV-infected individuals. The widely used genotyping assays have limitation with regard to genotype accuracy. This study was a comparative evaluation of exact HCV genotyping in a newly developed automated-massively parallel sequencing (MPS) system, versus the established Line probe assay 2...
August 26, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28797447/evaluation-of-the-xpert%C3%A2-hpv-assay-in-the-detection-of-human-papillomavirus-in-formalin-fixed-paraffin-embedded-oropharyngeal-carcinomas
#18
Maria Gabriella Donà, Francesca Rollo, Barbara Pichi, Giuseppe Spriano, Raul Pellini, Renato Covello, Edoardo Pescarmona, Giulia Fabbri, Manuela Scalfari, Tarik Gheit, Maria Benevolo
OBJECTIVES: The increasing incidence of HPV-related Oropharyngeal Squamous Cell Carcinoma (OPSCC) and the improved survival of HPV-positive OPSCC highlight the need for effective tools in evaluating HPV status on formalin-fixed paraffin-embedded (FFPE) cancers. To date, there is no agreement regarding the most appropriate method for HPV testing on FFPE materials. We aimed to investigate the performance of the Xpert® HPV assay (Cepheid) on crude lysates from OPSCC FFPE tissues. MATERIALS AND METHODS: Crude lysates were obtained by proteinase K digestion of FFPE tissues that had already been analyzed by the INNO-LiPA HPV assay and p16(ink4a) immunostaining...
September 2017: Oral Oncology
https://www.readbyqxmd.com/read/28782649/comparison-of-methods-available-for-identification-of-mycobacterium-chimaera
#19
E Lecorche, S Haenn, F Mougari, S Kumanski, N Veziris, H Benmansour, L Raskine, L Moulin, E Cambau
OBJECTIVES: Mycobacterium chimaera is a recently described nontuberculous mycobacterium belonging to the Mycobacterium avium complex (MAC). Because this species is implicated in a worldwide outbreak due to contaminated heater-cooler unit water tanks during open-heart surgery, it has become mandatory for clinical microbiology laboratories to be able to differentiate M. chimaera from the other MAC species, especially M. intracellulare. Such identification has so far been restricted to specialized laboratories because it required the analysis of several gene sequences...
August 4, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28782185/identification-of-hepatitis-c-virus-2k-1b-intergenotypic-recombinants-in-georgia
#20
Mamuka Zakalashvili, Jaba Zarkua, Michael Weizenegger, Jan Bartel, Monika Raabe, Lela Zangurashvili, Nino Kankia, Nino Jashiashvili, Maka Lomidze, Tengiz Telia, Vakhtang Kerashvili, Maia Zhamutashvili, Nikoloz Abramishvili, Charlotte Hedskog, Krishna Chodavarapu, Diana M Brainard, John G McHutchison, Hongmei Mo, Evguenia Svarovskaia, Robert G Gish, Irakli Rtskhiladze, David Metreveli
BACKGROUND AND AIMS: This study aimed to evaluate the prevalence of the hepatitis C virus intergenotype recombinant strain RF1_2k/1b in Georgia, confirm viral recombination by full genome sequencing, and determine a genetic relationship with previously described recombinant hepatitis C viruses. METHODS: We retrospectively analysed data from 1421 Georgian patients with chronic hepatitis C. Genotyping was performed with the INNO-LiPA VERSANT HCV Genotype 2.0 Assay...
August 7, 2017: Liver International: Official Journal of the International Association for the Study of the Liver
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