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https://www.readbyqxmd.com/read/29662627/analysis-of-the-pi3k-akt-mtor-pathway-in-penile-cancer-evaluation-of-a-therapeutically-targetable-pathway
#1
Anthony Adimonye, Elzbieta Stankiewicz, Sakunthala Kudahetti, Giorgia Trevisan, Brendan Tinwell, Cathy Corbishley, Yong-Jie Lu, Nick Watkin, Daniel Berney
Objectives: To determine whether phosphatidylinositol-4,5-bisphosphate 3- kinase, catalytic subunit alpha (PIK3CA) copy number gain is common and could prove a useful marker for the activation status of the PI3K-AKT-mTOR pathway in penile squamous cell carcinoma (PSCC). Methods: Fresh frozen tissue and archival blocks were collected from 24 PSCC patients with 15 matched normal penile epithelium (NPE) tissue from St George's Hospital. PIK3CA mutational and copy number status (CNS) was assessed via Sanger sequencing and fluorescence in-situ hybridisation, respectively...
March 23, 2018: Oncotarget
https://www.readbyqxmd.com/read/29622767/pex5-regulates-autophagy-via-the-mtorc1-tfeb-axis-during-starvation
#2
So Young Eun, Joon No Lee, In-Koo Nam, Zhi-Qiang Liu, Hong-Seob So, Seong-Kyu Choe, RaeKil Park
Defects in the PEX5 gene impair the import of peroxisomal matrix proteins, leading to nonfunctional peroxisomes and other associated pathological defects such as Zellweger syndrome. Although PEX5 regulates autophagy process in a stress condition, the mechanisms controlling autophagy by PEX5 under nutrient deprivation are largely unknown. Herein, we show a novel function of PEX5 in the regulation of autophagy via Transcription Factor EB (TFEB). Under serum-starved conditions, when PEX5 is depleted, the mammalian target of rapamycin (mTORC1) inhibitor TSC2 is downregulated, which results in increased phosphorylation of the mTORC1 substrates, including 70S6K, S6K, and 4E-BP-1...
April 6, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29596990/improved-inducible-expression-of-bacillus-naganoensis-pullulanase-from-recombinant-bacillus-subtilis-by-enhancer-regulation
#3
Yi Deng, Yao Nie, Yu Zhang, Yue Wang, Yan Xu
Pullulanase is crucial to the specific hydrolysis of branch points in amylopectin and is generally employed as an important enzyme in the starch-processing industry. Recombinant Bacillus subtilis that employs an inducible promoter would be a suitable candidate for pullulanase expression because of its safety and controllable production, but its level of pullulanase activity is relatively low. In this study, we investigated the effect of the enhancers DegQ, DegU, and DegS on pullulanase expression in a recombinant B...
March 26, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29587669/ezrin-and-e-cadherin-expression-profile-in-cervical-cytology-a-prognostic-marker-for-tumor-progression-in-cervical-cancer
#4
Ana E Zacapala-Gómez, Napoleón Navarro-Tito, Luz Del C Alarcón-Romero, Carlos Ortuño-Pineda, Berenice Illades-Aguiar, Eduardo Castañeda-Saucedo, Julio Ortiz-Ortiz, Olga L Garibay-Cerdenares, Marco A Jiménez-López, Miguel A Mendoza-Catalán
BACKGROUND: Cervical cancer (CC) is the fourth cause of mortality by neoplasia in women worldwide. The use of immunomarkers is an alternative tool to complement currently used algorithms for detection of cancer, and to improve selection of therapeutic schemes. Aberrant expression of Ezrin and E-cadherin play an important role in tumor invasion. In this study we analyzed Ezrin and E-cadherin expression in liquid-based cervical cytology samples, and evaluated their potential use as prognostic immunomarkers...
March 27, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29582635/possible-synergistic-interactions-among-multiple-hpv-genotypes-in-women-suffering-from-genital-neoplasia
#5
Massoud Hajia, Amir Sohrabi
Objective: Persistence of HPV infection is the true cause of cervical disorders. It is reported that competition may exist among HPV genotypes for colonization. This survey was designed to establish the multiple HPV genotype status in our community and the probability of multiple HPV infections involvement. Methods: All multiple HPV infections were selected for investigation in women suffering from genital infections referred to private laboratories in Tehran, Iran. A total of 160 multi HPV positive specimens from cervical scraping were identified by the HPV genotyping methods, "INNO-LiPA and Geno Array"...
March 27, 2018: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/29568110/compositional-associations-of-time-spent-in-sleep-sedentary-behavior-and-physical-activity-with-obesity-measures-in-children
#6
Robert Talarico, Ian Janssen
BACKGROUND/OBJECTIVES: The 24 h day is made up of four movement behaviors: sleep, sedentary behavior (SB), light intensity physical activity (LIPA), and moderate-to-vigorous physical activity (MVPA). Previous studies examining associations between movement behaviors and obesity have used flawed statistical methods that did not account for the co-dependent and compositional nature of movement behaviors. Our objective was to use compositional data analysis methods to investigate the relationships between movement behaviors and obesity measures among children...
March 5, 2018: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29566005/evaluation-of-the-cobas%C3%A2-gt-hepatitis-c-virus-genotyping-assay-in-g1-6-viruses-including-low-viral-loads-and-lipa-failures
#7
Benjamin Némoz, Léa Roger, Vincent Leroy, Jean-Dominique Poveda, Patrice Morand, Sylvie Larrat
Direct-acting antiviral (DAA) drug performances depend on the viral genotype. So international recommendations give typing of the virus a prerequisite for treatment choice and patient management. Commercially available HCV genotyping kits are scarce and this analysis is often in-house using tedious PCRs and Sanger sequencing, leading to a lack of standardization. A newly commercialized HCV genotyping assay based on real-time PCR has been developed by Roche Diagnostics (Mannheim, Germany). We compared this new assay with our in-house PCRs -sequencing technique on 101 regular samples and 81 LiPA failures or low viral load samples...
2018: PloS One
https://www.readbyqxmd.com/read/29561990/clinical-anthropometric-and-biochemical-characteristics-of-patients-with-or-without-genetically-confirmed-familial-hypercholesterolemia
#8
Andrea De Lorenzo, Juliana Duarte Lopes da Silva, Cinthia E James, Alexandre C Pereira, Annie Seixas Bello Moreira
BACKGROUND: Familial hypercholesterolemia (FH) is a common autosomal dominant disorder, characterized by a high level of low-density lipoprotein cholesterol (LDL-C) and a high risk of premature cardiovascular disease. OBJECTIVE: To evaluate clinical and anthropometric characteristics of patients with the familiar hypercholesterolemia (FH) phenotype, with or without genetic confirmation of FH. METHODS: Forty-five patients with LDL-C > 190 mg/dl were genotyped for six FH-related genes: LDLR, APOB, PCSK9, LDLRAP1, LIPA and APOE...
February 2018: Arquivos Brasileiros de Cardiologia
https://www.readbyqxmd.com/read/29558751/sudden-fetal-hematologic-changes-as-a-complication-of-amnioreduction-in-twin-twin-transfusion-syndrome
#9
Katarzyna Kosinska-Kaczynska, Michal Lipa, Iwona Szymusik, Dorota Bomba-Opon, Robert Brawura-Biskupski-Samaha, Szymon Kozlowski, Lisanne S A Tollenaar, Dick Oepkes, Miroslaw Wielgos, Enrico Lopriore
We present the first case of a monochorionic twin pregnancy in which sudden hematologic changes occurred as a complication of the amnioreduction procedure for twin-twin transfusion syndrome (TTTS). At 33 weeks of gestation, 4 days after the amnioreduction, the recipient developed severe anemia while the donor developed severe polycythemia. Postnatal placental examination revealed several arteriovenous and venoarterial anastomoses, a pale placental mass of the recipient and a congested and plethoric placental mass of the donor...
March 20, 2018: Fetal Diagnosis and Therapy
https://www.readbyqxmd.com/read/29551740/high-prevalence-of-anal-canal-high-risk-human-papillomavirus-infection-in-patients-with-crohn-s-disease
#10
Lucine Vuitton, Elise Jacquin, Anne-Laure Parmentier, Elise Crochet, Francine Fein, Anne-Claire Dupont-Gossart, Laurianne Plastaras, Charles-Henri Bretagne, Frédéric Mauny, Stéphane Koch, Jean-Luc Prétet, Christiane Mougin, Séverine Valmary-Degano
BACKGROUND & AIMS: The increasing incidence of anal canal carcinomas requires better knowledge on anal human papillomavirus (HPV) infection. We aimed to assess anal canal HPV infection prevalence and risk factors among patients seen at a gastroenterology department in France. METHODS: We analyzed anal tissue samples collected from 469 consecutive patients (median age 54 years, 52% women), including 112 who received immunosuppressant therapies and 101 with inflammatory bowel disease (70 with Crohn's disease), who underwent colonoscopy examinations from April 1, 2012 to April 30, 2015...
March 15, 2018: Clinical Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/29547398/lysosomal-acid-lipase-and-lipid-metabolism-new-mechanisms-new-questions-and-new-therapies
#11
Hanrui Zhang
PURPOSE OF REVIEW: Lysosomal acid lipase (LAL), encoded by the LIPA gene, is an essential lysosomal enzyme that hydrolyzes cholesteryl ester and triglyceride delivered to the lysosome. This review highlights the novel pathophysiological role of LAL, the functional genomic discoveries of LIPA as a risk locus for coronary heart diseases (CHD), and the clinical advance in therapies for LAL deficiency. RECENT FINDINGS: The essential role of LAL in lipid metabolism has been confirmed in human and mice with LAL deficiency...
March 15, 2018: Current Opinion in Lipidology
https://www.readbyqxmd.com/read/29546777/luminex-%C3%A2-xmap-%C3%A2-technology-is-an-effective-strategy-for-high-definition-human-leukocyte-antigen-typing-of-cord-blood-units-prior-to-listing
#12
Marco Guarene, Carla Badulli, Anna L Cremaschi, Ilaria Sbarsi, Rosalia Cacciatore, Carmine Tinelli, Annamaria Pasi, Paola Bergamaschi, Cesare G Perotti
INTRODUCTION: Allele-level donor-recipient match at HLA-A, HLA-B, HLA-C and HLA-DRB1 loci impacts the outcome after cord blood transplantation for hematologic malignancies and modifies the strategy of donor selection. High definition of both class I and II HLA loci at time of listing is a way to improve the attractiveness of cord blood bank inventories, reducing the time for donor search and procurement and simplifying donor choice, in particular, for patients of non-European heritage...
March 1, 2018: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/29527374/lysosomal-acid-lipase-deficiency-report-of-five-cases-across-the-age-spectrum
#13
Marco Antonio Curiati, Sandra Obikawa Kyosen, Vanessa Gonçalves Pereira, Francy Reis da Silva Patrício, Ana Maria Martins
Lysosomal acid lipase (LAL) deficiency is an autosomal recessive lysosomal storage disorder caused by mutations in the LIPA gene that leads to premature organ damage and mortality. We present retrospective data from medical records of 5 Brazilian patients, showing the broad clinical spectrum of the disease.
2018: Case Reports in Pediatrics
https://www.readbyqxmd.com/read/29523554/lysosomal-cholesterol-hydrolysis-couples-efferocytosis-to-anti-inflammatory-oxysterol-production
#14
Manon Viaud, Stoyan Ivanov, Nemanja Vujic, Madalina Duta-Mare, Lazaro-Emilio Aira, Thibault Barouillet, Elsa Garcia, François Orange, Isabelle Dugail, Isabelle Hainault, Christian Stehlik, Sandrine Marchetti, Laurent Boyer, Rodolphe Guinamard, Fabienne Foufelle, Andrea E Bochem, Kees G Hovingh, Edward B Thorp, Emmanuel L Gautier, Dagmar Kratky, Paul A Da Silva-Jardine, Laurent Yvan-Charvet
<u>Rationale:</u> Macrophages face a substantial amount of cholesterol following the ingestion of apoptotic cells and the lysosomal acid lipase (LIPA) has a major role in hydrolyzing cholesteryl esters in the endocytic compartment. <u>Objective:</u> Here, we directly investigated the role of LIPA-mediated clearance of apoptotic cells both in vitro and in vivo. <u>Methods and Results:</u> We show that LIPA inhibition causes a defective efferocytic response due to impaired generation of 25-OHC and 27-OHC...
March 9, 2018: Circulation Research
https://www.readbyqxmd.com/read/29522328/modification-of-lipase-with-poly-4-acryloylmorpholine-enhances-solubility-and-transesterification-activity-in-anhydrous-ionic-liquids
#15
Garrett R Chado, Elijah N Holland, Andrew K Tice, Mark P Stoykovich, Joel L Kaar
Tuning the molecular interaction between enzymes and their solvent environment through polymer modification can greatly improve activity and thus utility in biocatalytic reactions. In this work, this approach was exploited to enhance the activity of lipase A (LipA) from Bacillus subtilis in anhydrous ionic liquids (IL), which are highly attractive solvents for biocatalysis. Specifically, we showed that the transesterification activity of LipA in anhydrous 1-butyl-3-methyl imidazolium hexafluorophosphate ([BMIM][PF6]) was improved up to 19-fold via covalently conjugating the enzyme with the IL-soluble polymer poly(4-acryloylmorpholine) (PAcMO)...
March 9, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29516961/role-of-inferior-phrenic-artery-in-the-interventional-treatment-of-lung-metastases-tumor-a-report-of-11-cases
#16
Lin-Zhong Zhu, Ren-Jie Yang, Xu Zhu
Background: Lung metastases have been very common in advanced cancer, which were observed in 30%-40% of cancer cases. Transarterial chemoembolization (TACE) is one of the choice for treating lung cancer. In our center, 119 cases of lung metastases were treated with TACE, and we found that inferior phrenic artery (IPA) played an important role in this procedure. Materials and Methods: From June 2011 to June 2015, 119 cases with malignant lung metastases received TACE procedure in our center...
January 2018: Journal of Cancer Research and Therapeutics
https://www.readbyqxmd.com/read/29512741/macrophage-migration-inhibitory-factor-antagonist-s-r-3%C3%A2-4%C3%A2-hydroxyphenyl-%C3%A2-4-5%C3%A2-dihydro%C3%A2-5%C3%A2-isoxazole-acetic-acid-methyl-ester-attenuates-inflammation-and-lung-injury-in-rats-with-acute-pancreatitis-in-pregnancy
#17
Yu Zhou, Liang Zhao, Fangchao Mei, Yupu Hong, He Xia, Teng Zuo, Youming Ding, Weixing Wang
Macrophage migration inhibitory factor (MIF) is an inflammatory cytokine involved in many acute and chronic inflammatory diseases. However, its role in acute lung injury associated with acute pancreatitis in pregnancy (APIP) has not yet been elucidated. The present study was undertaken to clarify the effect and potential mechanism of MIF antagonist (S,R)3‑(4‑hydroxyphenyl)‑4,5‑dihydro‑5‑isoxazole acetic acid methyl ester (ISO‑1) in the development of acute lung injury in rats with APIP. Eighteen late‑gestation SD rats were randomly assigned to three groups: Sham operation (SO) group, APIP group, and ISO‑1 group...
March 1, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29462790/fungal-lignin-peroxidase-does-not-produce-the-veratryl-alcohol-cation-radical-as-a-diffusible-ligninolytic-oxidant
#18
Carl J Houtman, Eranda Maligaspe, Christopher G Hunt, Elena Fernandez-Fueyo, Angel T Martínez, Kenneth E Hammel
Peroxidases are considered essential agents of lignin degradation by white-rot basidiomycetes. However, low-molecular-weight oxidants likely have a primary role in lignin breakdown because many of these fungi delignify wood before its porosity has sufficiently increased for enzymes to infiltrate. It has been proposed that lignin peroxidases (LiPs, EC 1.11.1.14) fulfill this role by oxidizing the secreted fungal metabolite veratryl alcohol (VA) to its aryl cation radical (VA+• ), releasing it to act as a one-electron lignin oxidant within woody plant cell walls...
February 9, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29447902/the-erythrocyte-osmotic-resistance-test-as-screening-tool-for-cholesterol-related-lysosomal-storage-diseases
#19
Laura López de Frutos, Jorge J Cebolla, Pilar Irún, Ralf Köhler, Pilar Giraldo
BACKGROUND: Erythrocyte volume regulation and membrane elasticity are essential for adaptation to osmotic and mechanical stress, and life span. Here, we evaluated whether defective cholesterol trafficking caused by the rare lysosomal storages diseases (LSDs), Niemann-Pick type C (NPC) and Lysosomal acid lipase (LAL) deficiency (LALD) impairs these properties. Moreover, we tested whether measurements of cholesterol membrane content and osmotic resistance serve as a screening test for these LSDs...
February 12, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29440040/objectively-measured-physical-activity-sedentary-behaviour-and-all-cause-mortality-in-older-men-does-volume-of-activity-matter-more-than-pattern-of-accumulation
#20
Barbara J Jefferis, Tessa J Parsons, Claudio Sartini, Sarah Ash, Lucy T Lennon, Olia Papacosta, Richard W Morris, S Goya Wannamethee, I-Min Lee, Peter H Whincup
OBJECTIVES: To understand how device-measured sedentary behaviour and physical activity are related to all-cause mortality in older men, an age group with high levels of inactivity and sedentary behaviour. METHODS: Prospective population-based cohort study of men recruited from 24 UK General Practices in 1978-1980. In 2010-2012, 3137 surviving men were invited to a follow-up, 1655 (aged 71-92 years) agreed. Nurses measured height and weight, men completed health and demographic questionnaires and wore an ActiGraph GT3x accelerometer...
February 12, 2018: British Journal of Sports Medicine
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