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https://www.readbyqxmd.com/read/28797447/evaluation-of-the-xpert%C3%A2-hpv-assay-in-the-detection-of-human-papillomavirus-in-formalin-fixed-paraffin-embedded-oropharyngeal-carcinomas
#1
Maria Gabriella Donà, Francesca Rollo, Barbara Pichi, Giuseppe Spriano, Raul Pellini, Renato Covello, Edoardo Pescarmona, Giulia Fabbri, Manuela Scalfari, Tarik Gheit, Maria Benevolo
OBJECTIVES: The increasing incidence of HPV-related Oropharyngeal Squamous Cell Carcinoma (OPSCC) and the improved survival of HPV-positive OPSCC highlight the need for effective tools in evaluating HPV status on formalin-fixed paraffin-embedded (FFPE) cancers. To date, there is no agreement regarding the most appropriate method for HPV testing on FFPE materials. We aimed to investigate the performance of the Xpert® HPV assay (Cepheid) on crude lysates from OPSCC FFPE tissues. MATERIALS AND METHODS: Crude lysates were obtained by proteinase K digestion of FFPE tissues that had already been analyzed by the INNO-LiPA HPV assay and p16(ink4a) immunostaining...
September 2017: Oral Oncology
https://www.readbyqxmd.com/read/28782649/comparison-of-methods-available-for-the-identification-of-mycobacterium-chimaera
#2
Emmanuel Lecorche, Sophie Haenn, Faiza Mougari, Sylvain Kumanski, Nicolas Veziris, Hanaa Benmansour, Laurent Raskine, Laurent Moulin, Emmanuelle Cambau
OBJECTIVES: Mycobacterium chimaera is a recently described nontuberculous mycobacterium belonging to the M. avium complex (MAC). Because this species is implicated in a worldwide outbreak due to contaminated heater-cooler unit water tanks during open-heart surgery, it becomes mandatory for clinical microbiology laboratories to be able to differentiate M. chimaera from the other MAC species, especially M. intracellulare. Such identification was so far restricted to specialized laboratories, since it required the analysis of several gene sequences...
August 4, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28782185/identification-of-hepatitis-c-virus-2k-1b-intergenotypic-recombinants-in-georgia
#3
Mamuka Zakalashvili, Jaba Zarkua, Michael Weizenegger, Jan Bartel, Monika Raabe, Lela Zangurashvili, Nino Kankia, Nino Jashiashvili, Maka Lomidze, Tengiz Telia, Vakhtang Kerashvili, Maia Zhamutashvili, Nikoloz Abramishvili, Charlotte Hedskog, Krishna Chodavarapu, Diana M Brainard, John G McHutchison, Hongmei Mo, Evguenia Svarovskaia, Robert G Gish, Irakli Rtskhiladze, David Metreveli
BACKGROUND AND AIMS: This study aimed to evaluate the prevalence of the hepatitis C virus intergenotype recombinant strain RF1_2k/1b in Georgia, confirm viral recombination by full genome sequencing, and determine a genetic relationship to previously described recombinant hepatitis C viruses. METHODS: We retrospectively analyzed data from 1421 Georgian patients with chronic hepatitis C. Genotyping was performed with the INNO-LiPA VERSANT HCV Genotype 2.0 Assay. RESULTS: Virus isolates were assigned to nonspecific hepatitis C genotypes 2a/2c (n=387) as performed by sequencing of core and NS5B genes...
August 7, 2017: Liver International: Official Journal of the International Association for the Study of the Liver
https://www.readbyqxmd.com/read/28766104/protein-engineering-and-homologous-expression-of-serratia-marcescens-lipase-for-efficient-synthesis-of-a-pharmaceutically-relevant-chiral-epoxyester
#4
Ke-Cai Chen, Ming-Min Zheng, Jiang Pan, Chun-Xiu Li, Jian-He Xu
The lipase isolated from Serratia marcescens (LipA) is a useful biocatalyst for kinetic resolution of a pharmaceutically relevant epoxyester, (±)-3-(4'-methoxyphenyl) glycidic acid methyl ester [(±)-MPGM], to afford optically pure (-)-MPGM, a key intermediate for the synthesis of diltiazem hydrochloride. Two mutants, LipAL315S and LipAS271F, were identified from the combinatorial saturation mutation library of 14 amino acid residues lining the substrate-binding pocket. LipAL315S, LipAS271F, and their combination LipAL315S/S271F showed 2...
August 1, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28753662/ns3-genomic-sequencing-and-phylogenetic-analysis-as-alternative-to-a-commercially-available-assay-to-reliably-determine-hepatitis-c-virus-subtypes-1a-and-1b
#5
Karin Neukam, Alfredo P Martínez, Andrés C A Culasso, Ezequiel Ridruejo, Gabriel García, Federico A Di Lello
OBJECTIVE: To evaluate the use of hepatitis C virus (HCV) NS3 sequencing as alternative to the comercially available Versant HCV 2.0 reverse hybridization line-probe assay (LiPA 2.0) to determine HCV genotype 1 (HCV-1) subtypes. PATIENTS AND METHODS: A cohort of 104 patients infected by HCV-1 according to LiPA 2.0 was analyzed in a cross-sectional study conducted in patients seen from January 2012 to June 2016 at an outpatient clinic in Buenos Aires, Argentina. RESULTS: The samples were included within well supported subtype clades: 64 with HCV-1b and 39 with HCV-1a infection...
2017: PloS One
https://www.readbyqxmd.com/read/28749631/human-papillomavirus-hpv-genotype-distribution-in-patients-with-recurrent-respiratory-papillomatosis-rrp-in-iran
#6
Negin Sadat Eftekhaar, Mohammad Hadi Karbalaie Niya, Farzad Izadi, Sedigheh Teaghinezhad-S, Hossein Keyvani
Background: Currently, recurrent respiratory papillomatosis (RRP), common laryngeal warts in the upper airway systems of children and adults are on the increase. Human papillomaviruses (HPVs) are suspected as causative agents. This study concerned HPV incidence and genotype distribution in Iranian RRP patients. Methods: Specimens were collected from RRP patients referred to hospitals affiliated with Iran University of Medical Sciences, Tehran, Iran, from Dec 2014 to Feb 2016 in a cross sectional study. After DNA extraction with an QIAamp® DNA FFPE Tissue Kit, conventional PCR was performed and products were sequenced...
July 27, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28741709/prenatal-diagnosis-of-dural-sinus-malformation-in-a-fetus-presenting-with-general-oedema
#7
Michal Lipa, Przemyslaw Kosinski, Ritsuko K Pooh, Miroslaw Wielgos
Dural sinus malformation (DSM) is an extremely rare, congenital dilatation of the dural sinus pouch. DSM cases associated with arteriovenous shunt (AV shunt) usually have a poor pregnancy outcome due to an excessive hemodynamic load and progressive cardiac failure.
July 25, 2017: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28677147/looking-beyond-human-papillomavirus-hpv-genotype-16-and-18-defining-hpv-genotype-distribution-in-cervical-cancers-in-australia-prior-to-vaccination
#8
Julia M L Brotherton, Sepehr N Tabrizi, Samuel Phillips, Jan Pyman, Alyssa M Cornall, Neil Lambie, Lyndal Anderson, Margaret Cummings, Diane Payton, James P Scurry, Marsali Newman, Raghwa Sharma, Marion Saville, Suzanne M Garland
Australia has implemented a high-coverage HPV vaccination program but has not, to date, established the distribution of HPV types that occur in cervical cancers in Australia. This information is important for determining the potential for cervical cancer prevention with both current and broader spectrum HPV vaccines. We analysed 847 cervical cancers diagnosed 2005 to 2015 in tertiary centres in the three most populous Australian states with resolution of specimens containing multiple HPV types using laser-capture microdissection...
July 5, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28659158/neural-stem-cells-for-disease-modeling-of-wolman-disease-and-evaluation-of-therapeutics
#9
Francis Aguisanda, Charles D Yeh, Catherine Z Chen, Rong Li, Jeanette Beers, Jizhong Zou, Natasha Thorne, Wei Zheng
BACKGROUND: Wolman disease (WD) is a rare lysosomal storage disorder that is caused by mutations in the LIPA gene encoding lysosomal acid lipase (LAL). Deficiency in LAL function causes accumulation of cholesteryl esters and triglycerides in lysosomes. Fatality usually occurs within the first year of life. While an enzyme replacement therapy has recently become available, there is currently no small-molecule drug treatment for WD. RESULTS: We have generated induced pluripotent stem cells (iPSCs) from two WD patient dermal fibroblast lines and subsequently differentiated them into neural stem cells (NSCs)...
June 28, 2017: Orphanet Journal of Rare Diseases
https://www.readbyqxmd.com/read/28657227/prospective-comparison-of-hybrid-capture-2-and-spf%C3%A2-%C3%A2-lipa-for-carcinogenic-human-papillomavirus-detection-and-risk-prediction-of-cervical-cancer-a-population-based-cohort-study-in-china
#10
Li Dong, Rui Mei Feng, Li Zhang, Xiao Qian Xu, Xue Lian Zhao, Margaret Zhuoer Wang, You Lin Qiao, Fang Hui Zhao
OBJECTIVE: To investigate the extent of the cross-reactivity of hybrid capture 2 (HC2) assay and evaluate the potential effect of cross-reactivity on the long-term risk for cervical cancer and precancers. METHODS: Based on the Shanxi Province Cervical Cancer Screening Study-I (SPOCCS-I) cohort from 2005 to 2014 in Shanxi, China, SPF₁₀-line probe assay (LiPA) was performed in all 598 HC2 positive and 300 random-selected HC2 negative cervical specimens. Ten-year cumulative incidence rate (CIR) of cervical intraepithelial neoplasia grade 2 or worse (CIN2+) of these two tests was evaluated using Kaplan-Meier methods...
September 2017: Journal of Gynecologic Oncology
https://www.readbyqxmd.com/read/28655332/conditional-knock-out-of-lipoic-acid-protein-ligase-1-reveals-redundancy-pathway-for-lipoic-acid-metabolism-in-plasmodium-berghei-malaria-parasite
#11
Min Wang, Qiong Wang, Xiang Gao, Zhong Su
BACKGROUND: Lipoic acid is a cofactor for α-keto acid dehydrogenase system that is involved in the central energy metabolism. In the apicomplexan parasite, Plasmodium, lipoic acid protein ligase 1 (LplA1) and LplA2 catalyse the ligation of acquired lipoic acid to the dehydrogenase complexes in the mitochondrion. The enzymes LipB and LipA mediate lipoic acid synthesis and ligation to the enzymes in the apicoplast. These enzymes in the lipoic acid metabolism machinery have been shown to play important roles in the biology of Plasmodium parasites, but the relationship between the enzymes is not fully elucidated...
June 27, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28642584/molecular-switching-system-using-glycosylphosphatidylinositol-to-select-cells-highly-expressing-recombinant-proteins
#12
Emmanuel Matabaro, Zeng'an He, Yi-Shi Liu, Hui-Jie Zhang, Xiao-Dong Gao, Morihisa Fujita
Although many pharmaceutical proteins are produced in mammalian cells, there remains a challenge to select cell lines that express recombinant proteins with high productivity. Since most biopharmaceutical proteins are secreted by cells into the medium, it is difficult to select cell lines that produce large amounts of the target protein. To address this issue, a new protein expression system using the glycosylphosphatidylinositol (GPI)-anchor was developed. PGAP2 is involved in processing GPI-anchored proteins (GPI-APs) during transport...
June 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28615446/lysosome-mediated-degradation-of-a-distinct-pool-of-lipid-droplets-during-hepatic-stellate-cell-activation
#13
Maidina Tuohetahuntila, Martijn R Molenaar, Bart Spee, Jos F Brouwers, Richard Wubbolts, Martin Houweling, Cong Yan, Hong Du, Brian C VanderVen, Arie B Vaandrager, J Bernd Helms
Activation of hepatic stellate cells (HSCs) is a critical step in the development of liver fibrosis. During activation, HSCs lose their lipid droplets (LDs) containing triacylglycerols (TAGs), cholesteryl esters, and retinyl esters (REs). We previously provided evidence for the presence of two distinct LD pools, a preexisting and a dynamic LD pool. Here we investigate the mechanisms of neutral lipid metabolism in the preexisting LD pool. To investigate the involvement of lysosomal degradation of neutral lipids, we studied the effect of lalistat, a specific lysosomal acid lipase (LAL/Lipa) inhibitor on LD degradation in HSCs during activation in vitro The LAL inhibitor increased the levels of TAG, cholesteryl ester, and RE in both rat and mouse HSCs...
July 28, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28611758/genome-wide-analyses-reveal-genes-subject-to-positive-selection-in-pasteurella-multocida
#14
Peili Cao, Dongchun Guo, Jiasen Liu, Qian Jiang, Zhuofei Xu, Liandong Qu
Pasteurella multocida, a Gram-negative opportunistic pathogen, has led to a broad range of diseases in mammals and birds, including fowl cholera in poultry, pneumonia and atrophic rhinitis in swine and rabbit, hemorrhagic septicemia in cattle, and bite infections in humans. In order to better interpret the genetic diversity and adaptation evolution of this pathogen, seven genomes of P. multocida strains isolated from fowls, rabbit and pigs were determined by using high-throughput sequencing approach. Together with publicly available P...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28591813/the-energy-expenditure-benefits-of-reallocating-sedentary-time-with-physical-activity-a-systematic-review-and-meta-analysis
#15
Aviroop Biswas, Paul I Oh, Guy E Faulkner, Alis Bonsignore, Maureen T Pakosh, David A Alter
Background: We compared direct and daily cumulative energy expenditure (EE) differences associated with reallocating sedentary time to physical activity in adults for meaningful EE changes. Methods: Peer-reviewed studies in PubMed, Medline, EMBASE, CINAHL, PsycINFO, Cochrane Central Register of Controlled Trials and Cochrane Database of Systematic Reviews were searched from inception to March 2017. Randomized and non-randomized interventions with sedentary time and EE outcomes in adults were included...
June 7, 2017: Journal of Public Health
https://www.readbyqxmd.com/read/28561039/sedentary-behavior-and-compensatory-mechanisms-in-response-to-different-doses-of-exercise-a-randomized-controlled-trial-in-overweight-and-obese-adults
#16
E A Castro, P B Júdice, A M Silva, P J Teixeira, P J Benito
BACKGROUND/OBJECTIVES: To examine compensatory changes in sedentary behavior (SB) and light-intensity physical activities (LIPA) in response to a 22-week exercise training program in overweight/obese adults; and to determine if different forms of exercise training and physical activity recommendations interact with these compensatory changes. SUBJECTS/METHODS: Eighty-nine overweight and obese individuals (body mass index (BMI): 25-34.9 kg/m(2), 48% males), aged 18-50 years, were randomized into four intervention groups (strength, endurance, combined strength + endurance and physical activity recommendations) with a 25-30% caloric restriction of total daily energy expenditure for 22 weeks...
May 31, 2017: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28559511/diagnostic-performance-of-genotype-%C3%A2-mtbdrplus-line-probe-assay-in-bronchoalveolar-lavage-for-pulmonary-tuberculosis-diagnosis-in-sputum-scarce-and-smear-negative-patients
#17
Farah Idrees, Muhammad Irfan, Kauser Jabeen, Joveria Farooqi, Rumina Hasan
BACKGROUND: MTBDRplus line probe assay (LiPA) has been endorsed by the World Health Organization for pulmonary tuberculosis (TB) diagnosis. However, its value for Mycobacterium tuberculosis (MTB) detection in bronchoalveolar lavage (BAL) needs exploration. This study determined the diagnostic performance of MTBDRplus in BAL for MTB complex detection and isoniazid/rifampicin resistance in smear-negative and sputum scarce patients. MATERIALS AND METHODS: Retrospective evaluation of data (January-December 2013) from patients who underwent bronchoscopy was done...
April 2017: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28541628/high-risk-human-papillomavirus-genotype-distribution-and-attribution-to-cervical-cancer-and-precancerous-lesions-in-a-rural-chinese-population
#18
Xue Lian Zhao, Shang Ying Hu, Qian Zhang, Li Dong, Rui Mei Feng, Ross Han, Fang Hui Zhao
OBJECTIVE: To explore the genotype distribution of high-risk human papillomavirus (HR-HPV) and its attribution to different grades of cervical lesions in rural China, which will contribute to type-specific HPV screening tests and the development of new polyvalent HPV vaccines among the Chinese population. METHODS: One thousand two hundred ninety-two subjects were followed based on the Shanxi Province Cervical Cancer Screening Study I (SPOCCS-I), and screened by HPV DNA testing (hybrid capture® 2 [HC2]), liquid-based cytology (LBC), and if necessary, directed or random colposcopy-guided quadrant biopsies...
July 2017: Journal of Gynecologic Oncology
https://www.readbyqxmd.com/read/28539489/lipa-variants-in-genome-wide-association-studies-of-coronary-artery-diseases-loss-of-function-or-gain-of-function
#19
EDITORIAL
Hanrui Zhang, Muredach P Reilly
No abstract text is available yet for this article.
June 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28538091/wolman-disease-a-mimic-of-infant-leukemia
#20
Kaduveettil G Gopakumar, Priyakumari Thankamony, Sheela Nampoothiri, Deeksha Bali, Jubie Raj, Jayasudha A Vasudevan, Ramachandran K Nair
BACKGROUND: Infant leukemia most commonly present with pallor and hepatosplenomegaly. The possibility of other differential diagnosis also has to be kept in mind during evaluation, as identifying the precise etiology for this clinical presentation is crucial for management. OBSERVATION: An infant, was referred to us with suspected infant leukemia and was subsequently diagnosed to have lysosomal acid lipase deficiency/Wolman disease with a novel 5 bp deletion "c...
May 23, 2017: Journal of Pediatric Hematology/oncology
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