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https://www.readbyqxmd.com/read/28241697/-alterations-of-mirna-profiles-and-function-analysis-in-paraquat-induced-apoptosis-of-hnpcs
#1
M Huang, Q Cai, H H Li, D Lou
Objective: To investigate the impacts of paraquat on microRNA profiles in apoptosis of human neural progenitor cells (hNPCs) and to explore miRNA targets and biological functions. Methods: We used hNPCs as a popular in vitro cell model system for characterizing the neurotoxicity. Cell apoptosis was detected by Annexin V-APC/7-AAD after 24 h treatment with different concentrations of PQ (0, 5, 10, 20, 40, 80 μmol/L). Microarray profiling expression of PQ treated cell line and their corresponding control was determined and differentially expression miRNAs were confirmed by quantitatively real-time PCR...
January 20, 2017: Chinese Journal of Industrial Hygiene and Occupational Diseases
https://www.readbyqxmd.com/read/28241004/differential-sensitivity-of-target-genes-to-translational-repression-by-mir-17-92
#2
Hyun Yong Jin, Hiroyo Oda, Pengda Chen, Chao Yang, Xiaojuan Zhou, Seung Goo Kang, Elizabeth Valentine, Jennifer Kefauver, Lujian Liao, Yaoyang Zhang, Alicia Gonzalez-Martin, Jovan Shepherd, Gareth J Morgan, Tony S Mondala, Steven R Head, Pyeung-Hyeun Kim, Nengming Xiao, Guo Fu, Wen-Hsien Liu, Jiahuai Han, James R Williamson, Changchun Xiao
MicroRNAs (miRNAs) are thought to exert their functions by modulating the expression of hundreds of target genes and each to a small degree, but it remains unclear how small changes in hundreds of target genes are translated into the specific function of a miRNA. Here, we conducted an integrated analysis of transcriptome and translatome of primary B cells from mutant mice expressing miR-17~92 at three different levels to address this issue. We found that target genes exhibit differential sensitivity to miRNA suppression and that only a small fraction of target genes are actually suppressed by a given concentration of miRNA under physiological conditions...
February 27, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28240738/inflammation-induced-mirna-155-inhibits-self-renewal-of-neural-stem-cells-via-suppression-of-ccaat-enhancer-binding-protein-%C3%AE-c-ebp%C3%AE-expression
#3
Kayoko Obora, Yuta Onodera, Toshiyuki Takehara, John Frampton, Joe Hasei, Toshifumi Ozaki, Takeshi Teramura, Kanji Fukuda
Intracerebral inflammation resulting from injury or disease is implicated in disruption of neural regeneration and may lead to irreversible neuronal dysfunction. Analysis of inflammation-related microRNA profiles in various tissues, including the brain, has identified miR-155 among the most prominent miRNAs linked to inflammation. Here, we hypothesize that miR-155 mediates inflammation-induced suppression of neural stem cell (NSC) self-renewal. Using primary mouse NSCs and human NSCs derived from induced pluripotent stem (iPS) cells, we demonstrate that three important genes involved in NSC self-renewal (Msi1, Hes1 and Bmi1) are suppressed by miR-155...
February 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28240250/regulation-of-tfpi%C3%AE-expression-by-mir-27a-b-3p-in-human-endothelial-cells-under-normal-conditions-and-in-response-to-androgens
#4
Ana B Arroyo, Salam Salloum-Asfar, Carlos Pérez-Sánchez, Raúl Teruel-Montoya, Silvia Navarro, Nuria García-Barberá, Ginés Luengo-Gil, Vanessa Roldán, John-Bjarne Hansen, Chary López-Pedrera, Vicente Vicente, Rocío González-Conejero, Constantino Martínez
The increased risk of cardiovascular events in older men is multifactorial, but the significant reduction of testosterone levels has been involved. As this hormone regulates the expression of TFPI by unknown mechanisms, we aimed to evaluate the role of miRNAs in the regulation of TFPIα expression under normal conditions and in response to androgens. In silico studies allowed the selection of 4 miRNAs as potential TFPIα regulators. Only miR-27a/b-3p significantly reduced TFPIα expression in two endothelial cell lines...
February 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28239816/long-non-coding-rna-carlo-5-upregulation-associates-with-poor-prognosis-in-patients-suffering-gastric-cancer
#5
J-N Liu, Y-M Shangguan
OBJECTIVE: Accumulating evidence has demonstrated that microRNAs (miRNAs) play critical roles in cancer initiation and development. The present study aims to explore the clinical significance of long noncoding RNA CARLo-5 (CARLo-5) in gastric cancer. PATIENTS AND METHODS: CARLo-5 expression in normal gastric tissue, gastric cancer were quantified by Quantitative reverse transcription PCR. The relationship between CARLo-5 expression and the clinical features of gastric cancer was assessed...
February 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28239814/microrna15b-induced-smcc7721-apoptosis-via-down-regulation-of-xiap
#6
W-B Ding, Y-X Wang, C-W Dong
OBJECTIVE: microRNA can regulate cell growth, proliferation, and death; also, it can promote or inhibit cell growth and proliferation through regulation of apoptotic proteins. XIAP is a newly discovered pro-apoptotic protein, but how is XIAP regulated remains unclear. This study investigated the role of microRNA-15b in liver cancer cells' proliferation and apoptosis. MATERIALS AND METHODS: microRNA15b and control microRNA (miRNA) were purchased and transfected with hepatoma SMCC7721 cells using liposomal transfection techniques...
February 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28239651/profiling-of-circulating-micrornas-in-children-with-recent-onset-of-type-1-diabetes
#7
Suheda Erener, Ashish Marwaha, Rusung Tan, Constadina Panagiotopoulos, Timothy J Kieffer
Type 1 diabetes (T1D) is an autoimmune disease that is clinically silent until the majority of β cells are destroyed. There is an unmet need for reliable and cost-effective biomarkers to predict and diagnose diabetes at an early stage. A number of stable microRNAs (miRNAs) have been reported in serum and plasma and are now being investigated as biomarkers of different diseases. We measured the levels of 745 miRNAs in sera of children with recent-onset T1D and age-matched controls using locked nucleic acid-enhanced (LNA-enhanced) quantitative PCR profiling...
February 23, 2017: JCI Insight
https://www.readbyqxmd.com/read/28238835/hypoxia-regulates-microrna-expression-in-the-human-carotid-body
#8
Souren Mkrtchian, Kian Leong Lee, Jessica Kåhlin, Anette Ebberyd, Lorenz Poellinger, Malin Jonsson Fagerlund, Lars I Eriksson
The carotid body (CB) is the key sensing organ for physiological oxygen levels in the body. Under conditions of low oxygen (hypoxia), the CB plays crucial roles in signaling to the cardiorespiratory center in the medulla oblongata for the restoration of oxygen homeostasis. How hypoxia regulates gene expression in the human CB remains poorly understood. While limited information on transcriptional regulation in animal CBs is available, the identity and impact of important post-transcriptional regulators such as non-coding RNAs, and in particular miRNAs are not known...
February 23, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28238728/an-integrative-transcriptomic-analysis-reveals-bisphenol-a-exposure-induced-dysregulation-of-microrna-expression-in-human-endometrial-cells
#9
Wei-Chun Chou, Pei-Hsuan Lee, Yan-Yan Tan, Ho-Chen Lin, Chung-Wei Yang, Kuan-Hsueh Chen, Chun-Yu Chuang
Bisphenol A (BPA) are commonly used in the manufacture of polycarbonate plastics. Higher BPA exposure levels have been found in patients with endometrial hyperplasia that is one of risk factors of endometrial cancer (EC). Aberrant microRNAs (miRNAs) regulation has been observed in the development of cancer. Thus, this study investigated whether BPA exposure can disrupt miRNA regulation and its gene expression regarding to EC carcinogenic progress. Microarray experiments of miRNA and mRNA were performed in human endometrial cancer RL95-2 cells with treatment of low-to-moderate (10, 10(3) and 10(5)nM) BPA to explore the aberrant genes corresponding to human EC progression...
February 23, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28238240/altered-plasma-microrna-expression-in-patients-with-mitral-chordae-tendineae-rupture
#10
Mehmet Bulent Vatan, Aysel Kalaycı Yigin, Ramazan Akdemir, Mustafa Tarik Agac, Mehmet Akif Cakar, Murat Aksoy, Ersan Tatli, Harun Kilic, Huseyin Gunduz, Derya Guzel, Keziban Karacan
BACKGROUND: Mitral chordae tendineae rupture (MCTR) is a progressive disorder which leads to severe mitral regurgitation. Despite its importance, the precise pathogenetic mechanism of MCTR remains unclear. The study aim was to investigate the expression profile of circulating microRNAs (miRNAs) as being potentially involved in the development of MCTR. METHODS: Twenty-one patients with 'primary' MCTR, and 30 age- and gender-matched controls, were enrolled in the study...
September 2016: Journal of Heart Valve Disease
https://www.readbyqxmd.com/read/28237816/mir-146a-promotes-remyelination-in-a-cuprizone-model-of-demyelinating-injury
#11
Jing Zhang, Zheng Gang Zhang, Mei Lu, Xinli Wang, Xia Shang, Stanton B Elias, Michael Chopp
The death of mature oligodendrocytes (OLs) which are the sole myelinating cells of the central nervous system (CNS), leads to demyelination and functional deficits. Currently, there is lack of effective remyelination therapies for patients with demyelinating diseases. MicroRNAs (miRNAs) mediate OL function. We hypothesized that miR-146a, by inactivating interleukin-1 receptor-associated kinase 1 (IRAK1), promotes differentiation of oligodendrocyte progenitor cells (OPCs) and thereby enhances remyelination. To test this hypothesis, a demyelination model induced by a cuprizone (CPZ) diet was employed, in which C57BL/6J mice were fed with a CPZ diet for 5 weeks...
February 22, 2017: Neuroscience
https://www.readbyqxmd.com/read/28237528/identification-of-potential-therapeutic-target-genes-key-mirnas-and-mechanisms-in-oral-lichen-planus-by-bioinformatics-analysis
#12
Cuihua Gong, Shangtong Sun, Bing Liu, Jing Wang, Xiaodong Chen
The study aimed to identify the potential target genes and key miRNAs as well as to explore the underlying mechanisms in the pathogenesis of oral lichen planus (OLP) by bioinformatics analysis. The microarray data of GSE38617 were downloaded from Gene Expression Omnibus (GEO) database. A total of 7 OLP and 7 normal samples were used to identify the differentially expressed genes (DEGs) and miRNAs. The DEGs were then performed functional enrichment analyses. Furthermore, DEG-miRNA network and miRNA-function network were constructed by Cytoscape software...
February 12, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28236743/direct-binding-of-microrna-21-pre-element-with-regorafenib-an-alternative-mechanism-for-anti-colorectal-cancer-chemotherapy
#13
Xiaobing Chen, Bojian Xie, Liang Cao, Feng Zhu, Beibei Chen, Huifang Lv, Xingxing Fan, Lili Han, Liangyu Bie, Xinguang Cao, Xiaokun Shen, Feilin Cao
The Regorafenib is a broad-spectrum kinase inhibitor that has been approved to treat colorectal cancer (CRC). However, evidences have shown that the agent is also implicated in drug interaction with microRNA-21 (miR-21), an oncogenic miRNA which plays a key role in resisting programmed cell death in CRC cells. Here, we supposed that, instead of kinase inhibition, Regorafenib can directly bind to and then stabilize miR-21 pre-element, thus preventing RNase Dicer-meditated cleavage of the pre-element to mature miR-21...
February 10, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28236026/downregulation-of-mir-221-3p-contributes-to-il-1%C3%AE-induced-cartilage-degradation-by-directly-targeting-the-sdf1-cxcr4-signaling-pathway
#14
Xin Zheng, Feng-Chao Zhao, Yong Pang, Dong-Ya Li, Sheng-Cheng Yao, Shao-Song Sun, Kai-Jin Guo
Osteoarthritis (OA) is characterized by degradation of chondrocyte extracellular matrix (ECM). Accumulating evidence suggests that microRNAs (miRNAs) are associated with OA, but little is known of their function in chondrocyte ECM degradation. The objective of this study was to investigate the expression and function of miRNAs in OA. miRNA expression profile was determined in OA cartilage tissues and controls, employing Solexa sequencing and reverse transcription quantitative PCR (RT-qPCR). According to a modified Mankin scale, cartilage degradation was evaluated...
February 24, 2017: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/28235637/inhibition-of-mir-92d-3p-enhances-inflammation-responses-in-genetically-improved-farmed-tilapia-gift-oreochromis-niloticus-with-streptococcus-iniae-infection-by-modulating-complement-c3
#15
Jun Qiang, Yi-Fan Tao, Jie He, Hong-Xia Li, Pao Xu, Jin-Wen Bao, Yi-Lan Sun
MicroRNAs (miRNAs) are small, non-coding RNAs that regulate target gene expression by binding to the 3'-untranslated regions (3'-UTRs) of their target mRNAs. The miR-92 family is an important miRNA family, which was discovered to be related to regulation of tumor proliferation, apoptosis, invasion, and metastasis. Inhibition of miR-92d-3p was found previously in head kidney of genetically improved farmed tilapia (GIFT, Oreochromis niloticus) exposed to Streptococcus iniae infection. In this study, we found that miR-92d-3p regulated complement C3 mRNA levels by binding to its 3'-UTR by 3'-UTR luciferase reporter assay, and reduced miR-92d-3p expression resulted in increased C3 mRNA levels...
February 21, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28231299/exploration-of-inhibitory-mechanisms-of-curcumin-in-lung-cancer-metastasis-using-a-mirna-transcription-factor-target-gene-network
#16
De-Min Jiao, Li Yan, Li-Shan Wang, Hui-Zhen Hu, Xia-Li Tang, Jun Chen, Jian Wang, You Li, Qing-Yong Chen
The present study was aimed to unravel the inhibitory mechanisms of curcumin for lung cancer metastasis via constructing a miRNA-transcription factor (TF)-target gene network. Differentially expressed miRNAs between human high-metastatic non-small cell lung cancer 95D cells treated with and without curcumin were identified using a TaqMan human miRNA array followed by real-time PCR, out of which, the top 6 miRNAs (miR-302b-3p, miR-335-5p, miR-338-3p, miR-34c-5p, miR-29c-3p and miR-34a-35p) with more verified target genes and TFs than other miRNAs as confirmed by a literature review were selected for further analysis...
2017: PloS One
https://www.readbyqxmd.com/read/28230866/oridonin-induces-autophagy-via-inhibition-of-glucose-metabolism-in-p53-mutated-colorectal-cancer-cells
#17
Zhuo Yao, Fuhua Xie, Min Li, Zirui Liang, Wenli Xu, Jianhua Yang, Chang Liu, Hongwangwang Li, Hui Zhou, Liang-Hu Qu
The Warburg effect is an important characteristic of tumor cells, making it an attractive therapeutic target. Current anticancer drug development strategies predominantly focus on inhibitors of the specific molecular effectors involved in tumor cell proliferation. These drugs or natural compounds, many of which target the Warburg effect and the underlying mechanisms, still need to be characterized. To elucidate the anticancer effects of a natural diterpenoid, oridonin, we first demonstrated the anticancer activity of oridonin both in vitro and in vivo in colorectal cancer (CRC) cells...
February 23, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28230276/dissection-of-regulatory-elements-during-direct-conversion-of-somatic-cells-into-neurons
#18
Tahereh Soleimani, Nafiseh Falsafi, Hossein Fallahi
A revolutionary approach that involves direct conversion of somatic cells into almost any other types of cells showed promising results for regenerative medicine. Currently, producing valuable cell types including neurons, cardiomyocytes and hepatocytes through direct conversion of somatic cells appear to be a feasible option for regenerative medicine. The process involves inducing the cells by chemical cocktails or by expression of different types of transcription factors. In this concept, in vitro neurogenesis considered to be able to produce neuron cells to replace damaged neurons especially in Alzheimer and Parkinson disease...
February 23, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28230206/mirna-21-mediates-the-antiangiogenic-activity-of-metformin-through-targeting-pten-and-smad7-expression-and-pi3k-akt-pathway
#19
Mao Luo, Xiaoyong Tan, Lin Mu, Yulin Luo, Rong Li, Xin Deng, Ni Chen, Meiping Ren, Yongjie Li, Liqun Wang, Jianbo Wu, Qin Wan
Metformin, an anti-diabetic drug commonly used for type 2 diabetes therapy, is associated with anti-angiogenic effects in conditions beyond diabetes. miR-21 has been reported to be involved in the process of angiogenesis. However, the precise regulatory mechanisms by which the metformin-induced endothelial suppression and its effects on miR-21-dependent pathways are still unclear. Bioinformatic analysis and identification of miR-21 and its targets and their effects on metformin-induced antiangiogenic activity were assessed using luciferase assays, quantitative real-time PCR, western blots, scratch assays, CCK-8 assays and tubule formation assays...
February 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28229988/endometrial-microrna-signature-during-the-window-of-implantation-changed-in-patients-with-repeated-implantation-failure
#20
Cheng Shi, Huan Shen, Li-Juan Fan, Jing Guan, Xin-Bang Zheng, Xi Chen, Rong Liang, Xiao-Wei Zhang, Qing-Hua Cui, Kun-Kun Sun, Zhu-Ran Zhao, Hong-Jing Han
BACKGROUND: At present, a diagnostic tool with high specificity for impaired endometrial receptivity, which may lead to implantation failure, remains to be developed. We aimed to assess the different endometrial microRNA (miRNA) signatures for impaired endometrial receptivity by microarray analysis. METHODS: A total of 12 repeated implantation failure (RIF) patients and 10 infertile patients, who conceived and delivered after one embryo transfer attempt, were recruited as RIF and control groups, respectively...
2017: Chinese Medical Journal
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