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https://www.readbyqxmd.com/read/29160048/from-biodegradable-polymers-to-bioresorbable-vascular-scaffolds-available-evidence-in-the-current-era-of-new-generation-des
#1
Fabio Magliulo, Raffaele Piccolo, Anna Franzone, Giovanni Esposito, Gian B Danzi
New-generation drug-eluting stents (DES) encompass a large variety of coronary devices, featuring thin struts, biocompatible durable or biodegradable polymer coatings, and limus-eluting drugs. Due to improved early and long-term outcomes among patients undergoing percutaneous coronary intervention, new-generation metallic DES are recommended in almost all patient and lesion subsets. Available evidence from randomized trials indicate a similar safety and efficacy profile between biodegradable and durable polymers new-generation DES...
November 20, 2017: Minerva Cardioangiologica
https://www.readbyqxmd.com/read/29159853/the-use-of-cell-conditioned-medium-for-musculoskeletal-tissue-regeneration
#2
REVIEW
Francesca Veronesi, Veronica Borsari, Maria Sartori, Monia Orciani, Monica Mattioli-Belmonte Cima, Milena Fini
Tissue regenerative medicine combines the use of cells, scaffolds and molecules to repair damaged tissues. Different cell types are employed for musculoskeletal diseases, both differentiated and mesenchymal stromal cells (MSCs). In recent years, the hypothesis that cell-based therapy is guided principally by cell-secreted factors has become increasingly popular. The aim of the present literature review was to evaluate preclinical and clinical studies that used conditioned medium (CM), rich in cell-factors, for musculoskeletal regeneration with a cell-free approach...
November 20, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29159721/design-synthesis-in-silico-and-in-vitro-antimicrobial-screenings-of-novel-1-2-4-triazoles-carrying-1-2-3-triazole-scaffold-with-lipophilic-side-chain-tether
#3
Mohamed Reda Aouad, Mariem Mohammed Mayaba, Arshi Naqvi, Sanaa K Bardaweel, Fawzia Faleh Al-Blewi, Mouslim Messali, Nadjet Rezki
BACKGROUND: 1,2,4-Triazoles and 1,2,3-triazoles have gained significant importance in medicinal chemistry. RESULTS: This study describes a green, efficient and quick solvent free click synthesis of new 1,2,3-triazole-4,5-diesters carrying a lipophilic side chain via 1,3-dipolar cycloaddition of diethylacetylene dicarboxylate with different surfactant azides. Further structural modifications of the resulting 1,2,3-triazole diesters to their corresponding 1,2,4-triazole-3-thiones via multi-step synthesis has been also investigated...
November 21, 2017: Chemistry Central Journal
https://www.readbyqxmd.com/read/29156470/-functional-and-anatomic-outcomes-of-primary-and-secondary-internal-limiting-membrane-transplantation-in-large-and-persistent-macular-holes
#4
Jelka A Hess, Stephan Michels, Matthias D Becker
Background The gold standard therapy for full-thickness macular holes (FTMH) is vitrectomy (PPV) with peeling of the internal limiting membrane (ILM), gas tamponade of the vitreous cavity and postoperative face-down positioning. Nevertheless, eyes with large macular holes (> 400 µm) and surgical failures remain difficult to manage. Recently, ILM transplantation (ILM-TX) techniques were developed with acceptable results, advocating different mechanisms of hole closure: in such a setting, the ILM could serve as a scaffold for neuronal tissue in the pedicle ILM flap technique or the ILM could induce a contraction of the FTMH rims through shrinking of a folded ILM plug...
November 20, 2017: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/29145866/calcium-containing-scaffolds-induce-bone-regeneration-by-regulating-mesenchymal-stem-cell-differentiation-and-migration
#5
Rubén Aquino-Martínez, Alcira P Angelo, Francesc Ventura Pujol
BACKGROUND: Osteoinduction and subsequent bone formation rely on efficient mesenchymal stem cell (MSC) recruitment. It is also known that migration is induced by gradients of growth factors and cytokines. Degradation of Ca(2+)-containing biomaterials mimics the bone remodeling compartment producing a localized calcium-rich osteoinductive microenvironment. The aim of our study was to determine the effect of calcium sulfate (CaSO4) on MSC migration. In addition, to evaluate the influence of CaSO4 on MSC differentiation and the potential molecular mechanisms involved...
November 16, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29144001/biological-evaluation-and-energetic-analyses-of-novel-gsk-3%C3%AE-inhibitors
#6
Denan Zhang, Lei Liu, Lin Pang, Qing Jin, Kehui Ke, Ming Hu, Jingbo Yang, Weifang Ma, Hongbo Xie, Xiujie Chen
Glycogen synthase kinase-3 beta (GSK-3β) is involved in multiple signaling pathways. Consistent with its critical roles in normal cells, abnormalities in GSK-3β activity have been implicated in diabetes, heart disease, Parkinson disease and Alzheimer's disease. In this study, a series of new scaffolds of small molecule inhibitors of GSK-3β were identified by virtual screening and bioassay. Candidates that adhere to drug-like criteria from a virtual library of compounds were tested using computational docking studies...
November 16, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29139602/cell-yield-chondrogenic-potential-and-regenerated-cartilage-type-of-chondrocytes-derived-from-ear-nasoseptal-and-costal-cartilage
#7
Aijuan He, Huitang Xia, Kaiyan Xiao, Tingting Wang, Yu Liu, Jixin Xue, Dan Li, Shengjian Tang, Fangjun Liu, Xiaoyun Wang, Wenjie Zhang, Wei Liu, Yilin Cao, Guangdong Zhou
Functional reconstruction of large cartilage defects in subcutaneous sites remains clinically challenging because of limited donor cartilage. Tissue engineering is a promising and widely accepted strategy for cartilage regeneration. To date however, this strategy has not achieved a significant breakthrough in clinical translation owing to a lack of detailed pre-clinical data on cell yield and functionality of clinically applicable chondrocytes. To address this issue, the current study investigated the initial cell yield, proliferative potential, chondrogenic capacity, and regenerated cartilage-type of human chondrocytes derived from auricular (AU), nasoseptal (NS), and costal (CO) cartilage using a scaffold-free cartilage regeneration model (cartilage sheet)...
November 15, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29138501/targeting-sxip-eb1-interaction-an-integrated-approach-to-the-discovery-of-small-molecule-modulators-of-dynamic-binding-sites
#8
T B Almeida, A J Carnell, I L Barsukov, N G Berry
End binding protein 1 (EB1) is a key element in the complex network of protein-protein interactions at microtubule (MT) growing ends, which has a fundamental role in MT polymerisation. EB1 is an important protein target as it is involved in regulating MT dynamic behaviour, and has been associated with several disease states, such as cancer and neuronal diseases. Diverse EB1 binding partners are recognised through a conserved four amino acid motif, (serine-X-isoleucine-proline) which exists within an intrinsically disordered region...
November 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29134948/effect-of-scaffold-morphology-and-cell-co-culture-on-tenogenic-differentiation-of-hadmsc-on-centrifugal-melt-electrospun-poly-l%C3%A2-lactic-acid-fibrous-meshes
#9
Shaohua Wu, Hao Peng, Xiuhong Li, Philipp N Streubel, Yong Liu, Bin Duan
Engineered tendon grafts offer a promising alternative for grafting during the reconstruction of complex tendon tears. The tissue-engineered tendon substitutes have the advantage of increased biosafety and the option to customize their biochemical and biophysical properties to promote tendon regeneration. In this study, we developed a novel centrifugal melt electrospinning (CME) technique, with the goal of optimizing the fabrication parameters to generate fibrous scaffolds for tendon tissue engineering. The effects of CME processing parameters, including rotational speed, voltage, and temperature, on fiber properties (i...
November 14, 2017: Biofabrication
https://www.readbyqxmd.com/read/29133624/histopathological-histomorphometrical-and-radiological-evaluations-of-hydroxyapatite-bioactive-glass-and-fluorapatite-bioactive-glass-nanocomposite-foams-as-cell-scaffolds-in-rat-tibia-an-in-vivo-study
#10
Maryam Seyedmajidi, Sina Haghanifar, Karimollah Hajian-Tilaki, Seyedali Seyedmajidi
Bone defects are common and persistent problems in clinical orthopedics and dentistry. The development of synthetic reconstruction materials is essential owing to the restricted access to natural bone grafts, disease transmission risks, surgical costs, donor-site morbidity, infections, and immune response-related complications. Accordingly, the aim of the present study was to evaluate the histopathological, histomorphometrical, and radiological characteristics of composite foams containing hydroxyapatite/bioactive glass (HA/BG) and fluorapatite/bioactive glass (FA/BG) as cell scaffolds in rat tibia reconstruction...
November 14, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/29131142/a-consensus-model-of-human-apolipoprotein-a-i-in-its-monomeric-and-lipid-free-state
#11
John T Melchior, Ryan G Walker, Allison L Cooke, Jamie Morris, Mark Castleberry, Thomas B Thompson, Martin K Jones, Hyun D Song, Kerry-Anne Rye, Michael N Oda, Mary G Sorci-Thomas, Michael J Thomas, Jay W Heinecke, Xiaohu Mei, David Atkinson, Jere P Segrest, Sissel Lund-Katz, Michael C Phillips, W Sean Davidson
Apolipoprotein (apo)A-I is an organizing scaffold protein that is critical to high-density lipoprotein (HDL) structure and metabolism, probably mediating many of its cardioprotective properties. However, HDL biogenesis is poorly understood, as lipid-free apoA-I has been notoriously resistant to high-resolution structural study. Published models from low-resolution techniques share certain features but vary considerably in shape and secondary structure. To tackle this central issue in lipoprotein biology, we assembled a team of structural biologists specializing in apolipoproteins and set out to build a consensus model of monomeric lipid-free human apoA-I...
November 13, 2017: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/29128835/star-poly-%C3%AE%C2%B5-caprolactone-based-electrospun-fibers-as-biocompatible-scaffold-for-doxorubicin-with-prolonged-drug-release-activity
#12
Preethi Balakrishnan, Lorenza Gardella, Mahdi Forouharshad, Teresa Pellegrino, Orietta Monticelli
In this work, a novel drug delivery system consisting of poly(ε-caprolactone) (PCL) electrospun fibers containing an ad-hoc-synthesized star polymer made up of a poly(amido-amine) (PAMAM) core and PCL branches (PAMAM-PCL) was developed. The latter system which was synthesized via the ring opening polymerization of ε-caprolactone, starting from a hydroxyl-terminated PAMAM dendrimer and characterized by means of (1)H NMR, IR and DSC, was found to be compatible with both the polymer matrix and a hydrophilic chemotherapeutic drug, doxorubicin (DOXO), the model drug used in this work...
November 7, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29128541/a-pegylated-platelet-free-plasma-hydrogel-based-composite-scaffold-enables-stable-vascularization-and-targeted-cell-delivery-for-volumetric-muscle-loss
#13
Amit Aurora, Nicole Wrice, Thomas J Walters, Robert J Christy, Shanmugasundaram Natesan
Extracellular matrix (ECM) scaffolds are being used for the clinical repair of soft tissue injuries. Although improved functional outcomes have been reported, ECM scaffolds show limited tissue specific remodeling response with concomitant deposition of fibrotic tissue. One plausible explanation is the regression of blood vessels which may be limiting the diffusion of oxygen and nutrients across the scaffold. Herein we develop a composite scaffold as a vasculo-inductive platform by integrating PEGylated platelet free plasma (PFP) hydrogel with a muscle derived ECM scaffold (m-ECM)...
November 8, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29126441/tgf%C3%AE-3-recruits-endogenous-mesenchymal-stem-cells-to-initiate-bone-regeneration
#14
Moyuan Deng, Tieniu Mei, Tianyong Hou, Keyu Luo, Fei Luo, Aijun Yang, Bo Yu, Hao Pang, Shiwu Dong, Jianzhong Xu
BACKGROUND: The recruitment of a sufficient number of endogenous mesenchymal stem cells (MSCs) is the first stage of in-situ tissue regeneration. Transforming growth factor beta-3 (TGFβ3) could recruit stem or progenitor cells and endothelial cells to participate in tissue regeneration. However, the mechanism of TGFβ3 recruiting MSCs toward bone regeneration has remained obscure. METHODS: We estimated the promigratory property of TGFβ3 on human bone marrow MSCs (hBMSCs) cocultured with the vascular cells (human umbilical artery smooth muscle cells or human umbilical vein endothelial cells) or not by Transwell assay...
November 10, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29125698/engineering-potato-virus-x-particles-for-a-covalent-protein-based-attachment-of-enzymes
#15
Juliane Röder, Rainer Fischer, Ulrich Commandeur
Plant virus nanoparticles are often used to display functional amino acids or small peptides, thus serving as building blocks in application areas as diverse as nanoelectronics, bioimaging, vaccination, drug delivery, and bone differentiation. This is most easily achieved by expressing coat protein fusions, but the assembly of the corresponding virus particles can be hampered by factors such as the fusion protein size, amino acid composition, and post-translational modifications. Size constraints can be overcome by using the Foot and mouth disease virus 2A sequence, but the compositional limitations cannot be avoided without the introduction of time-consuming chemical modifications...
November 10, 2017: Small
https://www.readbyqxmd.com/read/29124934/can-relative-binding-free-energy-predict-selectivity-of-reversible-covalent-inhibitors
#16
Payal Chatterjee, Wesley M Botello-Smith, Han Zhang, Li Qian, Abdelaziz Alsamarah, David Kent, Jerome J Lacroix, Michel Baudry, Yun Luo
Reversible covalent inhibitors have many clinical advantages over noncovalent or covalent drugs. However, apart from selecting a warhead, substantial efforts in design and synthesis are needed to optimize noncovalent interactions to improve target-selective binding. Computational prediction of binding affinity for reversible covalent inhibitors presents a unique challenge since the binding process consists of multiple steps, which are not necessarily independent of each other. In this study, we lay out the relation between relative binding free energy and the overall reversible covalent binding affinity using a two-state binding model...
November 10, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29119734/tissue-engineered-composite-scaffold-of-poly-lactide-co-glycolide-and-hydroxyapatite-nanoparticles-seeded-with-autologous-mesenchymal-stem-cells-for-bone-regeneration
#17
Bing Zhang, Pei-Biao Zhang, Zong-Liang Wang, Zhong-Wen Lyu, Han Wu
OBJECTIVE: A new therapeutic strategy using nanocomposite scaffolds of grafted hydroxyapatite (g-HA)/ poly(lactide-co-glycolide) (PLGA) carried with autologous mesenchymal stem cells (MSCs) and bone morphogenetic protein-2 (BMP-2) was assessed for the therapy of critical bone defects. At the same time, tissue response and in vivo mineralization of tissue-engineered implants were investigated. METHODS: A composite scaffold of PLGA and g-HA was fabricated by the solvent casting and particulate-leaching method...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/29109782/berunda-polypeptides-multi-headed-fusion-proteins-promote-subcutaneous-administration-of-rapamycin-to-breast-cancer-in-vivo
#18
Jugal P Dhandhukia, Zhe Li, Santosh Peddi, Shruti Kakan, Arjun Mehta, David Tyrpak, Jordan Despanie, J Andrew MacKay
Recombinant Elastin-Like Polypeptides (ELPs) serve as attractive scaffolds for nanoformulations because they can be charge-neutral, water soluble, high molecular weight, monodisperse, biodegradable, and decorated with functional proteins. We recently reported that fusion of the FK-506 binding protein 12 (FKBP) to an ELP nanoparticle (FSI) reduces rapamycin (Rapa) toxicity and enables intravenous (IV) therapy in both a xenograft breast cancer model and a murine autoimmune disease model. Rapa has poor solubility, which leads to variable oral bioavailability or drug precipitation following parenteral administration...
2017: Theranostics
https://www.readbyqxmd.com/read/29104524/a-biocatalytic-route-to-highly-enantioenriched-%C3%AE-hydroxydioxinones
#19
Rick C Betori, Eric R Miller, Karl A Scheidt
A novel biocatalytic system to access a wide variety of β-hydroxydioxinones from β-ketodioxinones employing commercial engineered ketoreductases has been developed. This practical system provides a remarkably straightforward solution to limitations in accessing certain chemical scaffolds common in β-hydroxydioxinones that are of great interest due to their diversification capabilities. A few highlights of this system are that it is high yielding, highly enantioselective, and chromatography-free. We have demonstrated both a wide substrate scope and a high degree of scalability...
April 3, 2017: Advanced Synthesis & Catalysis
https://www.readbyqxmd.com/read/29103309/the-effect-of-nanofibre-based-polyethersulfone-pes-scaffold-on-the-chondrogenesis-of-human-induced-pluripotent-stem-cells
#20
Hossein Mahboudi, Masoud Soleimani, Seyed Ehsan Enderami, Mousa Kehtari, Hana Hanaee-Ahvaz, Hossein Ghanbarian, Mojgan Bandehpour, Shahrzad Nojehdehi Nojehdehi Sh, Samaneh Mirzaei, Bahram Kazemi
Human induced pluripotent stem cells (iPSCs) have been shown to have promising potential for regenerative medicine and tissue engineering applications. Chondrogenic differentiation of iPSCs is important for application in cartilage tissue engineering. In this study, we considered the effect of nanofibre-based polyethersulfone (PES) scaffold on the chondrogenesis of iPSCs. IPSC cells were cultured on the PES scaffold and scaffold free method. After 21 d, real-time PCR was performed to evaluate the cartilage-specific genes in the mRNA levels...
November 6, 2017: Artificial Cells, Nanomedicine, and Biotechnology
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