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https://www.readbyqxmd.com/read/29782947/multifunctional-degradable-electronic-scaffolds-for-cardiac-tissue-engineering
#1
Ron Feiner, Sharon Fleischer, Assaf Shapira, Or Kalish, Tal Dvir
The capability to on-line sense tissue function, provide stimulation to control contractility and efficiently release drugs within an engineered tissue microenvironment may enhance tissue assembly and improve the therapeutic outcome of implanted engineered tissues. To endow cardiac patches with such capabilities we developed elastic, biodegradable, electronic scaffolds. The scaffolds were composed of electrospun albumin fibers that served as both a substrate and a passivation layer for evaporated gold electrodes...
May 18, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29782946/delivery-of-the-improved-bmp-2-advanced-plasmid-dna-within-a-gene-activated-scaffold-accelerates-mesenchymal-stem-cell-osteogenesis-and-critical-size-defect-repair
#2
Rosanne M Raftery, Irene Mencía-Castaño, Simon Sperger, Gang Chen, Brenton Cavanagh, Georg Feichtinger, Heinz Redl, Ara Hacobian, Fergal J O'Brien
Gene-activated scaffolds have been shown to induce controlled, sustained release of functional transgene both in vitro and in vivo. Bone morphogenetic proteins (BMPs) are potent mediators of osteogenesis however we found that the delivery of plasmid BMP-2 (pBMP-2) alone was not sufficient to enhance bone formation. Therefore, the aim of this study was to assess if the use of a series of modified BMP-2 plasmids could enhance the functionality of a pBMP-2 gene-activated scaffold and ultimately improve bone regeneration when implanted into a critical sized bone defect in vivo...
May 18, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29781486/transition-metal-free-one-pot-double-c-h-functionalization-of-quinolines-with-disubstituted-electron-deficient-acetylenes
#3
B A Trofimov, K V Belyaeva, L P Nikitina, A G Mal'kina, A V Afonin, I A Ushakov, A V Vashchenko
Transition metal-free one-pot reaction of quinolines with acylarylacetylenes and water proceeds in the presence of KOH (55-60 °C, MeCN, 48 h) to afford 2-aryl-3-acylquinolines in up to 66% yield. Here, a formal replacement of the acetylene moiety by the aryl and acyl substituents in the quinoline scaffold takes place. In fact, it has been proved experimentally that the reaction involves the ring cleavage, accompanied by the rearrangement and insertion of the electron-deficient acetylene moiety to form a dihydroquinoline intermediate with an aldehyde functional group in position 4...
May 21, 2018: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29781171/fiber-based-fluorescence-lifetime-imaging-of-recellularization-processes-on-vascular-tissue-constructs
#4
Alba Alfonso-Garcia, Jeny Shklover, Benjamin E Sherlock, Alyssa Panitch, Leigh G Griffiths, Laura Marcu
New techniques able to monitor the maturation of tissue engineered constructs over time are needed for a more efficient control of developmental parameters. Here, a label-free fluorescence lifetime imaging (FLIm) approach implemented through a single fiber-optic interface is reported for non-destructive in situ assessment of vascular biomaterials. Recellularization processes of antigen removed bovine pericardium scaffolds with endothelial cells and mesenchymal stem cells were evaluated on the serous and the fibrous sides of the scaffolds, two distinct extracellular matrix niches, over the course of a 7 day culture period...
May 21, 2018: Journal of Biophotonics
https://www.readbyqxmd.com/read/29779446/repair-of-articular-osteochondral-defects-using-an-integrated-and-biomimetic-trilayered-scaffold
#5
Chenjun Zhai, Hao Fei, Junzheng Hu, Zhen Wang, Shun Xu, Qiang Zuo, Zeng Li, Zhen Wang, Wenwei Liang, Weimin Fan
Repair of articular cartilage defects using bilayered scaffolds is problematic because tissue-engineered cartilage is prone to overgrowth toward subchondral bone resulting in structural abnormalities of cartilage and subchondral bone. A "twice freeze-drying" technique was used to construct a dense isolation layer between the cartilage and subchondral bone layers in an integrated bilayered scaffold to prevent cartilage from excessive downgrowth. Briefly, beta-tricalcium phosphate was used for the subchondral bone layer of the scaffold, high-concentration chitosan/gelatin solution for the dense isolation layer, and low-concentration chitosan/gelatin solution for the cartilage layer...
May 19, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29766751/overcoming-challenges-in-engineering-large-scaffold-free-neocartilage-with-functional-properties
#6
Brian J Huang, Wendy E Brown, Thomas Keown, Jerry C Hu, Kyriacos A Athanasiou
Although numerous cartilage engineering methods have been described, few report generation of constructs greater than 4 cm2, which is the typical lesion size considered for cell-based therapies. Furthermore, current cell-based therapies only target focal lesions, while treatment of large non-isolated lesions remains an area of great demand. The objective of this study was to scale-up fabrication of self-assembled neocartilage from standard sizes of 0.2 cm2 to greater than 8 cm2. Passaged sheep articular chondrocytes were self-assembled into 5 mm or 25 mm dia...
May 16, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29765415/human-recombinant-peptide-sponge-enables-novel-less-invasive-cell-therapy-for-ischemic-stroke
#7
Michiyuki Miyamoto, Kentaro Nakamura, Hideo Shichinohe, Tomohiro Yamauchi, Masaki Ito, Hisayasu Saito, Masahito Kawabori, Toshiya Osanai, Tasuku Sasaki, Kiyohiro Houkin, Satoshi Kuroda
Bone marrow stromal cell (BMSC) transplantation has the therapeutic potential for ischemic stroke. However, it is unclear which delivery routes would yield both safety and maximal therapeutic benefits. We assessed whether a novel recombinant peptide (RCP) sponge, that resembles human collagen, could act as a less invasive and beneficial scaffold in cell therapy for ischemic stroke. BMSCs from green fluorescent protein-transgenic rats were cultured and Sprague-Dawley rats were subjected to permanent middle cerebral artery occlusion (MCAo)...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29758107/locking-the-non-template-dna-to-control-transcription
#8
Yuri Nedialkov, Dmitri Svetlov, Georgiy A Belogurov, Irina Artsimovitch
Universally conserved NusG/Spt5 factors reduce RNA polymerase pausing and arrest. In a widely accepted model, these proteins bridge the RNA polymerase clamp and lobe domains across the DNA channel, inhibiting the clamp opening to promote pause-free RNA synthesis. However, recent structures of paused transcription elongation complexes show that the clamp does not open and suggest alternative mechanisms of antipausing. Among these mechanisms, direct contacts of NusG/Spt5 proteins with the nontemplate DNA in the transcription bubble have been proposed to prevent unproductive DNA conformations and thus inhibit arrest...
May 14, 2018: Molecular Microbiology
https://www.readbyqxmd.com/read/29752108/surface-decorated-hydroxyapatite-scaffold-with-on-demand-delivery-of-dexamethasone-and-stromal-cell-derived-factor-1-for-enhanced-osteogenesis
#9
Bingjun Zhang, Hongyu Li, Lei He, Zhiwei Han, Teng Zhou, Wei Zhi, Xiaobo Lu, Xiong Lu, Jie Weng
In the process of bone regeneration, relatively early biological events including inflammatory response, angiogenesis, or stem cell homing, help the accompanying target actions of cell differentiation and calcification. Herein, we proposed a novel cell-guided tissue engineering system based on a surface-functionalized porous hydroxyapatite (HA) scaffolds with the ability to recruit cells and accelerate the differentiation of them along the osteoblastic lineage for optimizing large-sized bone defect repair. Inspired by microstructural properties of natural bone, HA scaffolds similar to the trabecular bone structure were prepared via a sugar sphere leaching technique, in which the inter-pore opening size was controllable...
August 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29751846/generation-of-three-dimensional-human-neuronal-cultures-application-to-modeling-cns-viral-infections
#10
Leonardo D'Aiuto, Jennifer Naciri, Nicholas Radio, Sesha Tekur, Dennis Clayton, Gerard Apodaca, Roberto Di Maio, Yun Zhi, Peter Dimitrion, Paolo Piazza, Matthew Demers, Joel Wood, Charleen Chu, Jason Callio, Lora McClain, Robert Yolken, James McNulty, Paul Kinchington, David Bloom, Vishwajit Nimgaonkar
BACKGROUND: A variety of neurological disorders including neurodegenerative diseases and infection by neurotropic viruses can cause structural and functional changes in the central nervous system (CNS), resulting in long-term neurological sequelae. An improved understanding of the pathogenesis of these disorders is important for developing efficacious interventions. Human induced pluripotent stem cells (hiPSCs) offer an extraordinary window for modeling pathogen-CNS interactions, and other cellular interactions, in three-dimensional (3D) neuronal cultures that can recapitulate several aspects of in vivo brain tissue...
May 11, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29751237/current-anti-diabetic-agents-and-their-molecular-targets-a-review
#11
REVIEW
Nagaraju Kerru, Ashona Singh-Pillay, Paul Awolade, Parvesh Singh
Diabetes mellitus is a medical condition characterized by the body's loss of control over blood sugar. The frequency of diagnosed cases and consequential increases in medical costs makes it a rapidly growing chronic disease that threatens human health worldwide. In addition, its unnerving statistical projections are perilous to both the economy of the nation and man's life expectancy. Type-I and type-II diabetes are the two clinical forms of diabetes mellitus. Type-II diabetes mellitus (T2DM) is illustrated by the abnormality of glucose homeostasis in the body, resulting in hyperglycemia...
May 3, 2018: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29750440/3d-architectures-of-co-x-p-using-silk-fibroin-scaffolds-an-active-and-stable-electrocatalyst-for-hydrogen-generation-in-acidic-and-alkaline-media
#12
Taek-Seung Kim, Hee Jo Song, Jae-Chan Kim, Bobae Ju, Dong-Wan Kim
Developing nonprecious, highly active, and stable catalysts is essential for efficient electrocatalytic hydrogen evolution reaction in water splitting. In this study, the facile synthesis of a 3D flower-like Cox P/carbon architecture is proposed composed of an assembly of nanosheets interconnected by silk fibroin that acts as 3D scaffolds and a carbon source. This unique 3D architecture coupled with a carbon matrix enhances catalytic activity by exposing more active sites and increasing charge transport. The flower-like Cox P/carbon can facilitate a lower overpotential, Tafel slope, charge transfer resistance, and a higher electrochemically active surface than carbon-free and silk-free Cox P...
May 10, 2018: Small
https://www.readbyqxmd.com/read/29745292/unraveling-the-molecular-mechanism-of-benzothiophene-and-benzofuran-scaffold-merged-compounds-binding-to-anti-apoptotic-myeloid-cell-leukemia-1
#13
Parthiban Marimuthu, Kalaimathy Singaravelu
Myeloid cell leukemia 1 (Mcl1), is an anti-apoptotic member of the Bcl-2 family proteins, has gained considerable importance due to its overexpression activity prevents the oncogenic cells to undergo apoptosis. This overexpression activity of Mcl1 eventually develops strong resistance to a wide variety of anticancer agents. Therefore, designing novel inhibitors with potentials to elicit higher binding affinity and specificity to inhibit Mcl1 activity is of greater importance. Thus, Mcl1 acts as an attractive cancer target...
May 10, 2018: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29742341/luminescence-tunable-polynorbornenes-for-simultaneous-multicolor-imaging-in-subcellular-organelles
#14
Nan Xie, Ke Feng, Jianqun Shao, Bin Chen, Chen-Ho Tung, Li-Zhu Wu
Through modular ROMP (ring-opening metathesis polymerization), biofunctional polynorbornenes are designed and fabricated from panchromatic fluorophores, bioactive peptides and PEG solubilizer for organelle-specific multicolor imaging. Attributed to the free permutation and combination of highly fluorescent red rhodamine B, green dichlorofluorescein and blue 9,10-diphenylanthracene fluorophores as well as signaling peptide sequences of Fx rFx K and TAT, we successfully realize simultaneous multicolor imaging toward lysosomes and mitochondria in living cells firstly utilizing polymeric scaffolds...
May 9, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29742311/organocatalyzed-domino-3-2-cycloaddition-payne-type-rearrangement-using-carbon-dioxide-and-epoxy-alcohols
#15
Arjan Willem Kleij, Sergio Sopeña, Mariachiara Cozzolino, Eduardo C Escudero-Adán, Marta Martínez Belmonte, Cristina Maquilón
An unprecedented organocatalytic approach towards highly substituted cyclic carbonates from tri- and tetra-substituted oxiranes and carbon dioxide has been developed. The protocol involves the use of a simple and cheap superbase under mild, additive- and metal-free conditions towards the initial formation of a less substituted carbonate product that equilibrates to a tri- or even tetra-substituted cyclic carbonate under thermodynamic control. The latter are conveniently trapped in situ providing overall a new domino process for synthetically elusive heterocyclic scaffolds...
May 9, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29741627/fast-sg-an-alignment-free-algorithm-for-hybrid-assembly
#16
Alex Di Genova, Gonzalo A Ruz, Marie-France Sagot, Alejandro Maass
Background: Long read sequencing technologies are the ultimate solution for genome repeats, allowing near reference level reconstructions of large genomes. However, long read de novo assembly pipelines are computationally intense and require a considerable amount of coverage, thereby hindering their broad application to the assembly of large genomes. Alternatively, hybrid assembly methods which combine short and long read sequencing technologies can reduce the time and cost required to produce de novo assemblies of large genomes...
May 5, 2018: GigaScience
https://www.readbyqxmd.com/read/29738867/pharmacoinformatics-approach-for-the-identification-of-polo-like-kinase-1-inhibitors-from-natural-sources-as-anti-cancer-agents
#17
Mohamed F AlAjmi, Md Tabish Rehman, Afzal Hussain, Gulam Mohmad Rather
Polo-like kinase-1 (PLK-1) plays a key role in cell cycle progression during mitosis. Overexpression/dysfunction of PLK-1 is directly associated with cancerous transformation and has been reported in different cancer types. Here, we employed high throughput virtual screening and molecular docking to screen Selleck's natural compound library against PLK-1 kinase domain. We have identified eight bioactive compounds (Apigenin, Dihydromyricetin, Diosmetin, Hesperidin, Hesperitin, Naringenin, Phlorizi, and Quercetin) as the potential inhibitors of PLK-1...
May 5, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29738248/regioselective-metalation-and-functionalization-of-the-pyrazolo-1-5-a-pyridine-scaffold-using-mg-and-zn-tmp-bases
#18
Moritz Balkenhohl, Bruno Salgues, Takahiro Hirai, Konstantin Karaghiosoff, Paul Knochel
A regioselective functionalization of the pyrazolo[1,5- a]pyridine scaffold using Mg- and Zn-TMP bases (TMP = 2,2,6,6-tetramethylpiperidyl) in the presence or absence of BF3 ·OEt2 is described. Also, various functionalized pyrazolo[1,5- a]pyridines bearing an ester function (and an NHBoc or ethyl group) are magnesiated and functionalized, leading to polysubstituted heterocycles. Additionally, a sulfoxide directed ortho-metalation, followed by the transition-metal-free amination of a pyrazolo[1,5- a]pyridine sulfoxide, using a magnesium amide, is reported...
May 8, 2018: Organic Letters
https://www.readbyqxmd.com/read/29737151/oxygen-releasing-antioxidant-cryogel-scaffolds-with-sustained-oxygen-delivery-for-tissue-engineering-applications
#19
Parvaiz Ahmad Shiekh, Anamika Singh, Ashok Kumar
With the advancement in biomaterial sciences, tissue engineered scaffolds are developing as a promising strategy for the regeneration of damaged tissues. However, only a few of these scaffolds have been translated into clinical applications. One of the primary drawbacks of the existing scaffolds is the lack of adequate oxygen supply within the scaffolds. Oxygen producing biomaterials have been developed as an alternate strategy but are faced with two major concerns. One is controlling the rate of oxygen generation, and the other is the production of reactive oxygen species...
May 8, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29736582/tissue-engineering-strategies-for-osteochondral-repair
#20
F Raquel Maia, Mariana R Carvalho, J Miguel Oliveira, Rui L Reis
Tissue engineering strategies have been pushing forward several fields in the range of biomedical research. The musculoskeletal field is not an exception. In fact, tissue engineering has been a great asset in the development of new treatments for osteochondral lesions. Herein, we overview the recent developments in osteochondral tissue engineering. Currently, the treatments applied in a clinical scenario have shown some drawbacks given the difficulty in regenerating a fully functional hyaline cartilage. Among the different strategies designed for osteochondral regeneration, it is possible to identify cell-free strategies, scaffold-free strategies, and advanced strategies, where different materials are combined with cells...
2018: Advances in Experimental Medicine and Biology
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