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https://www.readbyqxmd.com/read/28225218/synthesis-of-n-n-dialkylamino-nor-dihydroxanthene-hemicyanine-fused-near-infrared-fluorophores-and-their-first-water-soluble-and-or-bioconjugatable-analogues
#1
Jean-Alexandre Richard, Michelle Jui Hsien Ong, Sylvain Debieu, Mathieu Moreau, Anthony Romieu
The effective synthesis of extended conjugated N,N-dialkylamino-nor-dihydroxanthene-based fluorophores is described from diversely functionalized salicylic aldehydes. The access to these original fluorescent derivatives proceeded in two steps through a one-pot construction of the unusual nor-dihydroxanthene (nor-DHX) scaffold followed by a diversification step providing a wide variety of nor-DHX-hemicyanine fused dyes emitting in the range 730-790 nm. The versatility of our approach has enabled a further extension to the late-stage introduction of negatively/positively charged polar groups onto their terminal nitrogen heterocyclic subunit giving access to the first water-soluble and/or bioconjugatable members of this emerging class of NIR fluorophores...
February 22, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28221782/the-cation-%C3%AF-interaction-enables-a-halo-tag-fluorogenic-probe-for-fast-no-wash-live-cell-imaging-and-gel-free-protein-quantification
#2
Yu Liu, Kun Miao, Noah P Dunham, Hongbin Liu, Matthew Fares, Amie K Boal, Xiaosong Li, Xin Zhang
Design of fluorogenic probes for Halo-tag is highly desirable but challenging. Previous work achieved this goal by controlling the chemical switch of spirolactones upon the covalent conju-gation between Halo-tag and probes or by incorporating a 'channel dye' into the substrate bind-ing tunnel of Halo-tag. In this work, we have developed a novel class of Halo-tag fluorogenic probes that are derived from solvatochromic fluorophores. The optimal probe, harboring a ben-zothiadiazole scaffold, exhibits a 1000-fold fluorescence enhancement upon reaction with Halo-tag...
February 21, 2017: Biochemistry
https://www.readbyqxmd.com/read/28219011/inhibition-of-the-aldehyde-dehydrogenase-1-2-family-by-psoralen-and-coumarin-derivatives
#3
Cameron D Buchman, Thomas D Hurley
Aldehyde dehydrogenase 2 (ALDH2), one of 19 ALDH superfamily members, catalyzes the NAD+-dependent oxidation of aldehydes to their respective carboxylic acids. In this study, we further characterized the inhibition of four psoralen and coumarin derivatives towards ALDH2 and compared them to the ALDH2 inhibitor daidzin for selectivity against five ALDH1/2 isoenzymes. Compound 2 (Ki=19 nM) binds within the aldehyde-binding site of the free enzyme species of ALDH2. Thirty-three structural analogs were examined to develop a stronger SAR profile...
February 20, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28217993/assessment-of-label-free-quantification-in-discovery-proteomics-and-impact-of-technological-factors-and-natural-variability-of-protein-abundance
#4
Mhd Rami Al Shweiki, Susann Mönchgesang, Petra Majovsky, Domenika Thieme, Diana Trutschel, Wolfgang Hoehenwarter
We evaluated the state of label-free discovery proteomics focusing especially on technological contributions and contributions of naturally occurring differences in protein abundance to the inter-sample variability in protein abundance estimates in this highly peptide-centric technology. First, the performance of popular quantitative proteomics software, Proteome Discoverer, Scaffold, MaxQuant and Progenesis QIP was benchmarked using their default parameters and some modified settings. Beyond this the inter- sample variability in protein abundance estimates was decomposed into variability introduced by the entire technology itself and variable protein amounts inherent to individual plants of the Arabidopsis thaliana Col-0 accession...
February 20, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28214847/contribution-of-p62-to-phenotype-transition-of-coronary-arterial-myocytes-with-defective-autophagy
#5
Junxiang Bao, Guangbi Li, Xinxu Yuan, Erich Gulbins, Pin-Lan Li
BACKGROUND: Autophagy disorder contributes to dedifferentiation of arterial smooth muscle cells, but the mechanisms are poorly understood. Here, we sought to investigate the role of scaffolding adaptor p62/SQSTM1 (p62) in phenotype switching of mouse coronary arterial myocytes (CAMs) induced by CD38 gene deficiency or lysosomal dysfunction which blocks autophagic flux in the cells. METHODS: Protein expression was measured by western blot analysis and immunofluorescent staining...
3, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28212040/synthesis-and-speciation-dependent-properties-of-a-multimetallic-model-complex-of-nisod-that-exhibits-unique-hydrogen-bonding
#6
Ramsey A Steiner, Stephen P Dzul, Timothy L Stemmler, Todd C Harrop
The complex Na3[{Ni(II)(nmp)}3S3BTA(alk))] (1) (nmp(2-) = deprotonated form of N-(2-mercaptoethyl)picolinamide; H3S3BTA(alk) = N(1),N(3),N(5)-tris(2-mercaptoethyl)benzene-1,3,5-tricarboxamide, where H = dissociable protons), supported by the thiolate-benzenetricarboxamide scaffold (S3BTA(alk)), has been synthesized as a trimetallic model of nickel-containing superoxide dismutase (NiSOD). X-ray absorption spectroscopy (XAS) and (1)H NMR measurements on 1 indicate that the Ni(II) centers are square-planar with N2S2 coordination, and Ni-N and Ni-S distances of 1...
February 17, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28211875/a-hyaluronan-hydrogel-scaffold-based-xeno-free-culture-system-for-ex-vivo-expansion-of-human-corneal-epithelial-stem-cells
#7
D Chen, Y Qu, X Hua, L Zhang, Z Liu, S C Pflugfelder, D-Q Li
PurposeTo develop a hyaluronan hydrogel scaffold-based xeno-free culture system for ex vivo cultivation of human corneal epithelial stem cells (CESCs).Patients and MethodsCESCs were cultivated from donor limbal explants on the HyStem-C Hydrogel bio-scaffold in 12-well plates for 3 weeks. Group A used the traditional supplemented hormonal epidermal medium (SHEM) and group B used the defined SHEM (without fetal bovine serum and toxin A, adding 20% serum replacement). The growth and morphology of the cultured cells were assessed by phase contrast microscope...
February 17, 2017: Eye
https://www.readbyqxmd.com/read/28210971/scaffold-free-approach-produces-neocartilage-tissue-of-similar-quality-as-the-use-of-hystem%C3%A2-and-hydromatrix%C3%A2-scaffolds
#8
Janne H Ylärinne, Chengjuan Qu, Mikko J Lammi
Numerous biomaterials are being considered for cartilage tissue engineering, while scaffold-free systems have also been introduced. Thus, it is important to know do the scaffolds improve the formation of manufactured neocartilages. This study compares scaffold-free cultures to two scaffold-containing ones. Six million bovine primary chondrocytes were embedded in HyStem™ or HydroMatrix™ scaffolds, or suspended in scaffold-free chondrocyte culture medium, and then loaded into agarose gel supported culture well pockets...
April 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28208578/role-of-cortico-cancellous-heterologous-bone-in-human-periodontal-ligament-stem-cell-xeno-free-culture-studied-by-synchrotron-radiation-phase-contrast-microtomography
#9
Serena Mazzoni, Sara Mohammadi, Giuliana Tromba, Francesca Diomede, Adriano Piattelli, Oriana Trubiani, Alessandra Giuliani
This study was designed to quantitatively demonstrate via three-dimensional (3D) images, through the Synchrotron Radiation Phase-Contrast Microtomography (SR-PhC-MicroCT), the osteoinductive properties of a cortico-cancellous scaffold (Osteobiol Dual Block-DB) cultured with human Periodontal Ligament Stem Cells (hPDLSCs) in xeno-free media. In vitro cultures of hPDLSCs, obtained from alveolar crest and horizontal fibers of the periodontal ligament, were seeded onto DB scaffolds and cultured in xeno-free media for three weeks...
February 10, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28199884/three-dimensional-growth-of-human-endothelial-cells-in-an-automated-cell-culture-experiment-container-during-the-spacex-crs-8-iss-space-mission-the-spheroids-project
#10
Jessica Pietsch, Samuel Gass, Stefano Nebuloni, David Echegoyen, Stefan Riwaldt, Christin Baake, Johann Bauer, Thomas J Corydon, Marcel Egli, Manfred Infanger, Daniela Grimm
Human endothelial cells (ECs) were sent to the International Space Station (ISS) to determine the impact of microgravity on the formation of three-dimensional structures. For this project, an automatic experiment unit (EU) was designed allowing cell culture in space. In order to enable a safe cell culture, cell nourishment and fixation after a pre-programmed timeframe, the materials used for construction of the EUs were tested in regard to their biocompatibility. These tests revealed a high biocompatibility for all parts of the EUs, which were in contact with the cells or the medium used...
February 7, 2017: Biomaterials
https://www.readbyqxmd.com/read/28194961/universal-dynamic-dna-assembly-programmed-surface-hybridization-effect-for-single-step-reusable-and-amplified-electrochemical-nucleic-acid-biosensing
#11
Shufeng Liu, Li Fang, Yanqun Wang, Li Wang
The traditional sensitive electrochemical biosensors are commonly confronted with the cumbersome interface operation and washing procedures and the inclusion of extra exogenous reagents, which impose the challenge on the detection simplicity, reliability, and reusability. Herein, we present the proof-of-principle of a unique biosensor architecture based on dynamic DNA assembly programmed surface hybridization, which confers the single-step, reusable, and enzyme-free amplified electrochemical nucleic acid analysis...
February 10, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28194860/a-review-of-decellurization-methods-caused-by-an-urgent-need-for-quality-control-of-cell-free-extracellular-matrix-scaffolds-and-their-role-in-regenerative-medicine
#12
REVIEW
Marek Kawecki, Wojciech Łabuś, Agnieszka Klama-Baryla, Diana Kitala, Malgorzata Kraut, Justyna Glik, Marcelina Misiuga, Mariusz Nowak, Tomasz Bielecki, Aleksandra Kasperczyk
The natural extracellular matrix (ECM),thanks to its specific properties (e.g., collagenous lattice, a reservoir of growth factors, ECM-cell anchoring areas, an optimal pH and CO2 ),ensures an optimal microenvironment for homeostatic and regenerative cell development. In the context of regenerative medicine, ECM is a lair for residual and infiltrative cells. The aim of the clinical usage of cell-free ECM scaffolds is the enhancement of tissue regeneration with possible minimization of an adverse host reaction on allogeneic or xenogeneic biomaterial...
February 14, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28193145/constrained-cage-culture-improves-engineered-cartilage-functional-properties-by-enhancing-collagen-network-stability
#13
Robert J Nims, Alexander D Cigan, Krista M Durney, Brian K Jones, John D O'Neill, Wing-Sum A Law, Gordana Vunjak-Novakovic, Clark Tung-Hui Hung, Gerard A Ateshian
When cultured with sufficient nutrient supply, engineered cartilage synthesizes proteoglycans rapidly, producing an osmotic swelling pressure that destabilizes immature collagen and prevents the development of a robust collagen framework, a hallmark of native cartilage. We hypothesized that mechanically constraining the proteoglycan-induced tissue swelling would enhance construct functional properties through the development of a more stable collagen framework. To test this hypothesis, we developed a novel "cage" growth system to mechanically prevent tissue constructs from swelling while ensuring adequate nutrient supply to the growing construct...
February 14, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28192527/the-efficacy-of-a-scaffold-free-bio-3d-conduit-developed-from-human-fibroblasts-on-peripheral-nerve-regeneration-in-a-rat-sciatic-nerve-model
#14
Hirofumi Yurie, Ryosuke Ikeguchi, Tomoki Aoyama, Yukitoshi Kaizawa, Junichi Tajino, Akira Ito, Souichi Ohta, Hiroki Oda, Hisataka Takeuchi, Shizuka Akieda, Manami Tsuji, Koichi Nakayama, Shuichi Matsuda
BACKGROUND: Although autologous nerve grafting is the gold standard treatment of peripheral nerve injuries, several alternative methods have been developed, including nerve conduits that use supportive cells. However, the seeding efficacy and viability of supportive cells injected in nerve grafts remain unclear. Here, we focused on a novel completely biological, tissue-engineered, scaffold-free conduit. METHODS: We developed six scaffold-free conduits from human normal dermal fibroblasts using a Bio 3D Printer...
2017: PloS One
https://www.readbyqxmd.com/read/28192154/electrospun-polyurethane-nanofiber-scaffolds-with-ciprofloxacin-oligomer-effect-on-drug-release-and-cell-attachment-versus-free-drug
#15
Meghan Ee Wright, Ian C Parrag, Meilin Yang, J Paul Santerre
An electrospun degradable polycarbonate urethane (PCNU) nanofiber scaffold loaded with antibiotic was investigated in terms of antibacterial efficacy and cell compatibility for potential use in gingival tissue engineering. Antimicrobial oligomer (AO), a compound which consists of two molecules of ciprofloxacin (CF) covalently bound via hydrolysable linkages to triethylene glycol (TEG), was incorporated via a one-step blend electrospinning process using a single solvent system at 7 and 15% w/w equivalent CF with respect to the PCNU...
February 10, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28186213/multifunctional-biocompatible-and-ph-responsive-carbon-nanotube-and-graphene-oxide-tectomer-hybrid-composites-and-coatings
#16
Rosa Garriga, Izabela Jurewicz, Shayan Seyedin, Niki Bardi, Stella Totti, Brigitta Matta-Domjan, Eirini G Velliou, Mohammed A Alkhorayef, Vicente L Cebolla, Joselito M Razal, Alan B Dalton, Edgar Muñoz
Here we present a route for non-covalent functionalization of carboxylated multi-walled carbon nanotubes and graphene oxide with novel two-dimensional peptide assemblies. We show that self-assembled amino-terminated biantennary and tetraantennary oligoglycine peptides (referred to as tectomers) effectively coat carboxylated multi-walled carbon nanotubes and also strongly interact with graphene oxide due to electrostatic interactions and hydrogen bonding as the driving force, respectively. The resulting hybrids can be made into free-standing conducting composites or applied in the form of thin, pH-switchable bioadhesive coatings onto graphene oxide fibers...
February 10, 2017: Nanoscale
https://www.readbyqxmd.com/read/28181209/heparin-modification-of-a-biomimetic-bone-matrix-modulates-osteogenic-and-angiogenic-cell-response-in-vitro
#17
M Quade, S Knaack, D Weber, U König, B Paul, P Simon, A Rösen-Wolff, R Schwartz-Albiez, M Gelinsky, A Lode
In this study, the effect of heparin-modified collagen type I/hydroxyapatite (HA) nanocomposites on key processes of bone regeneration - osteogenesis and angiogenesis - was characterised in vitro. Two approaches were applied for heparin modification: it was either integrated during material synthesis (in situ) or added to the porous scaffolds after their fabrication (post). Cultivation of human bone marrow-derived stromal cells (hBMSC), in heparin-modified versus heparin-free scaffolds, revealed a positive effect of the heparin modification on their proliferation and osteogenic differentiation...
February 9, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28180061/kinase-suppressor-of-ras-2-ksr2-expression-in-the-brain-regulates-energy-balance-and-glucose-homeostasis
#18
Lili Guo, Diane L Costanzo-Garvey, Deandra R Smith, Beth K Neilsen, Richard G MacDonald, Robert E Lewis
OBJECTIVE: Kinase Suppressor of Ras 2 (KSR2) is a molecular scaffold coordinating Raf/MEK/ERK signaling that is expressed at high levels in the brain. KSR2 disruption in humans and mice causes obesity and insulin resistance. Understanding the anatomical location and mechanism of KSR2 function should lead to a better understanding of physiological regulation over energy balance. METHODS: Mice bearing floxed alleles of KSR2 (KSR2(fl/fl)) were crossed with mice expressing the Cre recombinase expressed by the Nestin promoter (Nes-Cre) to produce Nes-CreKSR2(fl/fl) mice...
February 2017: Molecular Metabolism
https://www.readbyqxmd.com/read/28177642/transition-metal-free-amine-oxidation-a-chemoselective-strategy-for-the-late-stage-formation-of-lactams
#19
Robert J Griffiths, Glenn A Burley, Eric P A Talbot
A metal-free strategy for the formation of lactams via selective oxidation of cyclic secondary and tertiary amines is described. Molecular iodine facilitates both chemoselective and regioselective oxidation of C-H bonds directly adjacent to a cyclic amine. The mild conditions, functional group tolerance, and substrate scope are demonstrated using a suite of diverse small molecule cyclic amines, including clinically approved drug scaffolds.
February 8, 2017: Organic Letters
https://www.readbyqxmd.com/read/28174781/complexes-of-mn2s2%C3%A2-fe-%C3%AE-5-c5r5-co-as-platform-for-exploring-cooperative-heterobimetallic-effects-in-her-electrocatalysis
#20
Pokhraj Ghosh, Manuel Quiroz, Ning Wang, Nattamai Bhuvanesh, Marcetta Y Darensbourg
The control of aggregation at sulfur by metallodithiolates (MN2S2) has made them prime candidates as building blocks for the synthesis of biomimetics of various bimetallic enzyme active sites, with reactivity consequences implicating redox control by both metal centers. Recent studies of MN2S2 (M = Ni(2+), Fe(NO)(2+)) bound to [(η(5)-C5H5)Fe(CO)](+) as electrocatalysts for proton reduction, the hydrogen evolution reaction, demonstrated reduction-induced hemi-lability of the bridging cis-dithiolates as a key step in the electrochemical proton reduction process (Ding, et al...
February 8, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
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