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https://www.readbyqxmd.com/read/28527695/cytoprotection-proliferation-and-epidermal-differentiation-of-adipose-tissue-derived-stem-cells-on-emu-oil-based-electrospun-nanofibrous-mat
#1
Younes Pilehvar-Soltanahmadi, Mohammad Nouri, Mikaël M Martino, Amir Fattahi, Effat Alizadeh, Masoud Darabi, Mohammad Rahmati-Yamchi, Nosratollah Zarghami
Electrospun nanofibrous scaffolds containing natural substances with wound healing properties such as Emu oil (EO) may have a great potential for increasing the efficiency of stem cell-based skin bioengineering. For this purpose, EO blended PCL/PEG electrospun nanofibrous mats were successfully fabricated and characterized using FE-SEM, FTIR and Universal Testing Machine. The efficiency of the scaffolds in supporting the adherence, cytoprotection, proliferation and differentiation of adipose tissue-derived stem cells (ADSCs) to keratinocyte was evaluated...
May 17, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28525708/affinity-based-assembly-of-peptides-on-plasmonic-nanoparticles-delivered-intracellularly-with-light-activated-control
#2
Demosthenes P Morales, William Wonderly, Xiao Huang, Meghan McAdams, Amanda Chron, Norbert O Reich
We report a universal strategy for functionalizing near-infrared light-responsive nanocarriers with both a peptide "cargo" and an orthogonal cell-penetrating peptide. Modularity of both the cargo and internalization peptide attachment are an important feature of these materials relying on the robust affinity of polyhistidine tags to nitrilotriacetic acid in the presence of nickel as well as the affinity of biotin labeled peptides to streptavidin. Attachment to the gold surface uses thiol labeled scaffolds terminated with the affinity partner...
May 19, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28522639/definition-of-a-rack1-interaction-network-in-drosophila-melanogaster-using-swath-ms
#3
Lauriane Kuhn, Karim Majzoub, Evelyne Einhorn, Johana Chicher, Pompon Julien, Jean-Luc Imler, Philippe Hammann, Carine Meignin
Receptor for Activated C kinase 1 (RACK1) is a scaffold protein that has been found in association with several signaling complexes, and with the 40S subunit of the ribosome. Using the model organism Drosophila melanogaster, we recently showed that RACK1 is required at the ribosome for IRES-mediated translation of viruses. Here, we report a proteomic characterization of the interactome of RACK1 in Drosophila S2 cells. We carried out Label-Free quantitation using both Data-Dependent and Data-Independent Acquisition and observed a significant advantage for the Sequential Window Acquisition of all THeoretical fragment-ion spectra (SWATH) method both in terms of identification of interactants and quantification of low abundance proteins...
May 18, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28518068/3d-magnetic-stem-cell-aggregation-and-bioreactor-maturation-for-cartilage-regeneration
#4
Aurore Van de Walle, Claire Wilhelm, Nathalie Luciani
Cartilage engineering remains a challenge due to the difficulties in creating an in vitro functional implant similar to the native tissue. An approach recently explored for the development of autologous replacements involves the differentiation of stem cells into chondrocytes. To initiate this chondrogenesis, a degree of compaction of the stem cells is required; hence, we demonstrated the feasibility of magnetically condensing cells, both within thick scaffolds and scaffold-free, using miniaturized magnetic field sources as cell attractors...
April 27, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28516460/taxane-grafted-metal-oxide-nanoparticles-as-a-new-theranostic-tool-against-cancer-the-promising-example-of-docetaxel-functionalized-titanate-nanotubes-on-prostate-tumors
#5
Alexis Loiseau, Julien Boudon, Céline Mirjolet, Gilles Créhange, Nadine Millot
The combination of anticancer drugs and metal oxide nanoparticles is of great interest in cancer nanomedicine. Here, the development of a new nanohybrid, titanate nanotube-docetaxel (TiONts-DTX) is reported, the two parts of which are conjugated by covalent linkages. Unlike most nanoparticles currently being developed for biomedical purposes, TiONts present a needle-shaped morphology. The surface of TiONts is linked with 3-aminopropyl triethoxysilane and with a hetero-bifunctional polymer (polyethylene glycol) to create well-dispersed and biocompatible nanovectors...
May 18, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28508073/three-dimensional-system-enabling-the-maintenance-and-directed-differentiation-of-pluripotent-stem-cells-under-defined-conditions
#6
Denise Zujur, Kosuke Kanke, Alexander C Lichtler, Hironori Hojo, Ung-Il Chung, Shinsuke Ohba
The development of in vitro models for the maintenance and differentiation of pluripotent stem cells (PSCs) is an active area of stem cell research. The strategies used so far are based mainly on two-dimensional (2D) cultures, in which cellular phenotypes are regulated by soluble factors. We show that a 3D culture system with atelocollagen porous scaffolds can significantly improve the outcome of the current platforms intended for the maintenance and lineage specification of mouse PSCs (mPSCs). Unlike 2D conditions, the 3D conditions maintained the undifferentiated state of mouse embryonic stem cells (mESCs) without exogenous stimulation and also supported endoderm, mesoderm, and ectoderm differentiation of mESCs under serum-free conditions...
May 2017: Science Advances
https://www.readbyqxmd.com/read/28504242/simultaneous-microfluidic-spinning-of-multiple-strands-of-submicron-fiber-for-the-production-of-free-standing-porous-membranes-for-biological-application
#7
DoYeun Park, JiSoo Park, Heeyeong Jang, Jie Cheng, Soo-Hyun Kim, Sang-Hoon Lee
Microfibers produced using electrospinning and microfluidics-based technologies have been developed as a powerful tool in tissue engineering applications such as drug delivery and scaffolds. The applications of these fibers, however, have been limited because of the hazardous solvents used to make them, difficulties in controlling the pore sizes of their membrane forms, and downscaling the size of the fiber. Nevertheless, extending the use of these fibers such as the production of a free-standing porous membrane appropriate for cell-based research is highly needed for tissue engineering, organ-on-a-chip, and drug delivery research and applications...
May 15, 2017: Biofabrication
https://www.readbyqxmd.com/read/28504224/denmark-coronary-and-structural-heart-interventions-from-2010-to-2015
#8
Hans-Henrik Tilsted, Ole Ahlehoff, Christian J Terkelsen, Frants Pedersen, Cengiz Özcan, Troels H Jørgensen, Jens E Nielsen-Kudsk, Jan Ravkilde, Henrik Nissen, Sune A Pedersen, Ole Havndrup, Jens F Lassen
Interventional cardiology in Denmark has been carried out since the mid 1980s. Interventional cardiology is only performed at a few high-volume centres. Healthcare coverage is universal and is essentially free of charge. Hospitals are mostly publicly owned and financed by fixed budgets and, in part, an activity-based funding system. Approximately 30,000 coronary angiographies (CAG), 10,000 percutaneous coronary interventions (PCIs) of which approximately 25% are primary PCIs, and 500 transcatheter aortic valve implantations (TAVIs) are carried out each year...
May 15, 2017: EuroIntervention
https://www.readbyqxmd.com/read/28503405/nanoparticle-design-strategies-for-effective-cancer-immunotherapy
#9
Praveena Velpurisiva, Aniket Gad, Brandon Piel, Rahul Jadia, Prakash Rai
Cancer immunotherapy is a rapidly evolving and paradigm shifting treatment modality that adds a strong tool to the collective cancer treatment arsenal. It can be effective even for late stage diagnoses and has already received clinical approval. Tumors are known to not only avoid immune surveillance but also exploit the immune system to continue local tumor growth and metastasis. Because of this, most immunotherapies, particularly those directed against solid cancers, have thus far only benefited a small minority of patients...
2017: J Biomed (Syd)
https://www.readbyqxmd.com/read/28500664/ligand-promoted-rhodium-iii-catalyzed-ortho-c-h-amination-with-free-amines
#10
Huai-Wei Wang, Yi Lu, Bing Zhang, Jian He, Hua-Jin Xu, Yan-Shang Kang, Wei-Yin Sun, Jin-Quan Yu
Ligand development for Rh(III)-catalyzed C-H activation reactions are largely limited to Cp based scaffolds. 2-Methylquinoline is identified as a feasible ligand which can coordinate to the metal center of Cp*RhCl to accelerate the cleavage of C-H bond of N-pentafluorophenylbenzamides, providing a new structural lead for ligand design. The compatibility of this reaction with secondary free amines and anilines also overcomes the limitations of Pd(II)-catalyzed C-H amination reactions.
May 12, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28500524/cytoskeletal-proteins-in-caulobacter-crescentus-spatial-orchestrators-of-cell-cycle-progression-development-and-cell-shape
#11
Kousik Sundararajan, Erin D Goley
Caulobacter crescentus, an aquatic Gram-negative α-proteobacterium, is dimorphic, as a result of asymmetric cell divisions that give rise to a free-swimming swarmer daughter cell and a stationary stalked daughter. Cell polarity of vibrioid C. crescentus cells is marked by the presence of a stalk at one end in the stationary form and a polar flagellum in the motile form. Progression through the cell cycle and execution of the associated morphogenetic events are tightly controlled through regulation of the abundance and activity of key proteins...
2017: Sub-cellular Biochemistry
https://www.readbyqxmd.com/read/28498040/pivotal-role-of-non-cardiomyocytes-in-electromechanical-and-therapeutic-potential-of-induced-pluripotent-stem-cell-derived-engineered-cardiac-tissue
#12
Hiroko Iseoka, Shigeru Miyagawa, Satsuki Fukushima, Atsuhiro Saito, Shigeo Masuda, Shin Yajima, Emiko Ito, Nagako Sougawa, Maki Takeda, Akima Harada, Jong-Kook Lee, Yoshiki Sawa
Although engineered cardiac tissues (ECTs) derived from induced pluripotent stem cells (iPSCs) are promising for myocardial regenerative therapy, the appropriate ratio of cardiomyocytes to non-cardiomyocytes is not fully understood. Here, we determined whether ECT-cell content is a key determinant of its structure/function, thereby affecting ECT therapeutic potential for advanced heart failure. Scaffold-free ECTs containing different ratios (25%, 50%, 70%, or 90%) of iPSC-derived cardiomyocytes were generated by magnetic-activated cell sorting using cardiac-specific markers...
May 12, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28490814/matrix-elasticity-modified-scaffold-loaded-with-sdf-1%C3%AE-improves-the-in-situ-regeneration-of-segmental-bone-defect-in-rabbit-radius
#13
Guobao Chen, Yonggang Lv
The effectiveness of stem-cell based therapy has been hampered by the limited availability of stem cell sources, immune rejection, and difficulties in clinical adoption and regulatory approval. These obstacles can be partially circumvented by using in situ tissue engineering that recruits the endogenous stem/progenitor cells and provides cues to direct stem cell phenotype. Here, decellularized bone scaffold is mechanically modified by coating of collagen (Col)/hydroxyapatite (HA) mixture with optimal ratio and loaded with chemokine stromal cell-derived factor-1α (SDF-1α), in which endogenous stem cell recruitment can be improved by chemokine and stem cell fate can be regulated by matrix elasticity of the scaffold...
May 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28486802/synthesis-and-characterization-of-diol-based-unsaturated-polyesters-poly-diol-fumarate-and-poly-diol-fumarate-co-succinate
#14
Alexander M Tatara, Emma Watson, Tejus Satish, David W Scott, Dimitrios P Kontoyiannis, Paul S Engel, Antonios G Mikos
In this work, we describe the synthesis and characterization of variants of poly(diol fumarate) and poly(diol fumarate-co-succinate). Through a Fischer esterification, α,ω-diols and dicarboxylic acids were polymerized to form aliphatic polyester comacromers. Because of the carbon-carbon double bond of fumaric acid, incorporating it into the macromer backbone structure resulted in unsaturated chains. By choosing α,ω-diols of different lengths (1,6-hexanediol, 1,8-octanediol, and 1,10-decanediol) and controlling the amount of fumaric acid in the dicarboxylic acid monomer feed (33, 50, and 100 mol %), nine diol-based macromer variants were synthesized and characterized for molecular weight, number of unsaturated bonds per chain, and thermal properties...
May 17, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28486019/role-of-bone-marrow-mononuclear-cell-seeding-for-nanofiber-vascular-grafts
#15
Takuma Fukunishi, Cameron A Best, Chin Siang Ong, Tyler Groehl, James Reinhardt, Tai Yi, Hideki Miyachi, Huaitao Zhang, Toshiharu Shinoka, Christopher K Breuer, Jed Johnson, Narutoshi Hibino
OBJECTIVE: Electrospinning is a promising technology that provides biodegradable nanofiber scaffolds for cardiovascular tissue engineering. However, success with these materials has been limited, and the optimal combination of scaffold parameters for a tissue-engineered vascular graft (TEVG) remains elusive. The purpose of the present study is to evaluate the effect of bone marrow mononuclear cell (BM-MNC) seeding in electrospun scaffolds to support the rational design of optimized TEVGs...
May 9, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28485490/label-free-relative-quantification-of-secreted-proteins-as-a-non-invasive-method-for-the-quality-control-of-chondrogenesis-in-bioengineered-substitutes-for-cartilage-repair
#16
Christel Henrionnet, Pierre Gillet, Didier Mainard, Jean-Baptiste Vincourt, Astrid Pinzano
Cartilage tissue engineering is making progress but the competing available strategies still leave room for improvement and consensual overviews regarding the best combinations of scaffolds and cell sources are limited by the capacity to compare them directly. In addition, because most strategies involve autologous cell transfer, once these will be optimized, the resulting implants require individual quality control prior to grafting in order to emphasize patient-to-patient differential responsiveness to engineering processes...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28485205/effect-of-epigallocatechin-3-gallate-on-the-increase-in-type-ii-collagen-accumulation-in-cartilage-like-msc-sheets
#17
Keigo Sato, Hisashi Mera, Shigeyuki Wakitani, Mutsumi Takagi
With the aim to increase type II collagen content in the scaffold-free cartilage-like cell sheet using human bone marrow mesenchymal stem cells, we examined the effect of epigallocatechin-3-gallate (EGCG) addition to the chondrogenic medium for the cell sheet culture. The addition of EGCG (10 μM) increased the content of type II collagen 2-fold, while the addition did not markedly change the expression level of the genes encoding type II collagen and Sox 9. The reactive oxygen species level in the cells in cell sheets was thought to be too low to suppress the accumulation of type II collagen...
June 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28483696/spatial-distributions-of-pericellular-stiffness-in-natural-extracellular-matrices-are-dependent-on-cell-mediated-proteolysis-and-contractility
#18
M Keating, A Kurup, M Alvarez-Elizondo, A J Levine, E Botvinick
Bulk tissue stiffness has been correlated with regulation of cellular processes and conversely cells have been shown to remodel their pericellular tissue according to a complex feedback mechanism critical to development, homeostasis, and disease. However, bulk rheological methods mask the dynamics within a heterogeneous fibrous extracellular matrix (ECM) in the region proximal to a cell (pericellular region). Here, we use optical tweezers active microrheology (AMR) to probe the distribution of the complex material response function (α = α' + α'', in units of µm/nN) within a type I collagen ECM, a biomaterial commonly used in tissue engineering...
May 5, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28481437/effective-light-directed-assembly-of-building-blocks-with-microscale-control
#19
Ngoc-Duy Dinh, Rongcong Luo, Maria Tankeh Asuncion Christine, Weikang Nicholas Lin, Wei-Chuan Shih, James Cho-Hong Goh, Chia-Hung Chen
Light-directed forces have been widely used to pattern micro/nanoscale objects with precise control, forming functional assemblies. However, a substantial laser intensity is required to generate sufficient optical gradient forces to move a small object in a certain direction, causing limited throughput for applications. A high-throughput light-directed assembly is demonstrated as a printing technology by introducing gold nanorods to induce thermal convection flows that move microparticles (diameter = 40 µm to several hundreds of micrometers) to specific light-guided locations, forming desired patterns...
May 8, 2017: Small
https://www.readbyqxmd.com/read/28480130/depolymerizable-poly-o-vinyl-carbamate-alt-sulfones-as-customizable-macromolecular-scaffolds-for-mucosal-drug-delivery
#20
Kaushlendra Kumar, Eduard Jimenez Castaño, Andrew R Weidner, Adem Yildirim, Andrew P Goodwin
Interest in stimulus responsive materials and polymers has grown over the years, having shown great promise in a diverse set of applications. For drug delivery, stimulus-responsive polymers have been shown to encapsulate therapeutic cargo such as small molecule drugs or proteins, deliver them to specific locations in the body, and release them so that they can induce a therapeutic effect in the patient. Most hydrolytically degradable polymers are synthesized via nucleophilic, anionic, or cationic polymerization, which generally requires protection of nucleophilic or protic side chains prior to polymerization...
May 17, 2016: ACS Macro Letters
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