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https://www.readbyqxmd.com/read/27918018/a-planar-model-of-the-vessel-wall-from-cellularized-collagen-scaffolds-focus-on-cell-matrix-interactions-in-mono-bi-and-tri-culture-models
#1
Caroline Loy, Sébastien Meghezi, Lucie Lévesque, Daniele Pezzoli, Heena Kumra, Dieter Reinhardt, Jayachandran N Kizhakkedathu, Diego Mantovani
The acquisition of new thorough knowledge on the interactions existing between vascular cells would represent a step forward in the engineering of vascular tissues. In this light, herein we designed a physiological-like tri-culture in vitro vascular wall model using a planar cellularized collagen gel as the scaffold. The model can be obtained in 24 h and features multi-layered hierarchical organization composed of a fibroblast-containing adventitia-like layer, a media-like layer populated by smooth muscle cells and an intima-like endothelial cell monolayer...
December 5, 2016: Biomaterials Science
https://www.readbyqxmd.com/read/27917703/principles-of-the-kenzan-method-for-robotic-cell-spheroid-based-3d-bioprinting
#2
Nicanor I Moldovan, Narutoshi Hibino, Koichi Nakayama
Bioprinting is a technology with the prospect to change the way many diseases are treated, by replacing the damaged tissues with live, de novo created bio-similar constructs. However, after more than a decade of incubation and many proofs-of-concept, the field is still in its infancy. The current stagnation is the consequence of its early success: the first bioprinters, and most of those which followed, were modified versions of the 3D printers used in additive manufacturing, redesigned for layer-by-layer dispersion of biomaterials...
December 4, 2016: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/27914984/recapitulating-the-human-tumor-microenvironment-colon-tumor-derived-extracellular-matrix-promotes-angiogenesis-and-tumor-cell-growth
#3
Mónica Romero-López, Andrew L Trinh, Agua Sobrino, Michaela M S Hatch, Mark T Keating, Cristhian Fimbres, David E Lewis, Paul D Gershon, Elliot L Botvinick, Michelle Digman, John S Lowengrub, Christopher C W Hughes
Extracellular matrix (ECM) is an essential and dynamic component of all tissues and directly affects cellular behavior by providing both mechanical and biochemical signaling cues. Changes in ECM can alter tissue homeostasis, potentially leading to promotion of cellular transformation and the generation of tumors. Therefore, understanding ECM compositional changes during cancer progression is vital to the development of targeted treatments. Previous efforts to reproduce the native 3D cellular microenvironment have utilized protein gels and scaffolds that incompletely recapitulate the complexity of native tissues...
November 24, 2016: Biomaterials
https://www.readbyqxmd.com/read/27907087/prenylated-polyphenols-from-clusiaceae-and-calophyllaceae-with-immunomodulatory-activity-on-endothelial-cells
#4
Caroline Rouger, Sylvain Pagie, Séverine Derbré, Anne-Marie Le Ray, Pascal Richomme, Béatrice Charreau
Endothelial cells (ECs) are key players in inflammation and immune responses involved in numerous pathologies. Although attempts were experimentally undertaken to prevent and control EC activation, drug leads and probes still remain necessary. Natural products (NPs) from Clusiaceous and Calophyllaceous plants were previously reported as potential candidates to prevent endothelial dysfunction. The present study aimed to identify more precisely the molecular scaffolds that could limit EC activation. Here, 13 polyphenols belonging to 5 different chemical types of secondary metabolites (i...
2016: PloS One
https://www.readbyqxmd.com/read/27895039/region-specific-effect-of-the-decellularized-meniscus-extracellular-matrix-on-mesenchymal-stem-cell-based-meniscus-tissue-engineering
#5
Kazunori Shimomura, Benjamin B Rothrauff, Rocky S Tuan
BACKGROUND: The meniscus is the most commonly injured knee structure, and surgical repair is often ineffective. Tissue engineering-based repair or regeneration may provide a needed solution. Decellularized, tissue-derived extracellular matrices (ECMs) have received attention for their potential use as tissue-engineered scaffolds. In considering meniscus-derived ECMs (mECMs) for meniscus tissue engineering, it is noteworthy that the inner and outer regions of the meniscus have different structural and biochemical features, potentially directing the differentiation of cells toward region-specific phenotypes...
November 28, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27888739/microwave-assisted-one-pot-synthesis-characterization-biological-evaluation-and-molecular-docking-studies-of-steroidal-thiazoles
#6
Mohd Asif, Abad Ali, Atif Zafar, Mohd Farhan, Hena Khanam, S M Hadi, Shamsuzzaman
In the present manuscript, a series of steroidal thiazole derivatives (4-6, 8) have been synthesized in efficient manner by one step reaction methodology employing microwave irradiation. The synthesis involves the reaction of steroidal ketones (1-3, 7) with thiosemicarbazide and phenacyl bromide. Structural assignment of the products was confirmed on the basis of IR, (1)H NMR, (13)C NMR, MS and analytical data. The synthesized compounds were screened for in vitro antioxidant activity by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method...
November 15, 2016: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/27885807/loading-of-bmp2-related-peptide-onto-three-dimensional-nano-hydroxyapatite-scaffolds-accelerates-mineralization-in-critical-sized-cranial-bone-defects
#7
Tingfang Sun, Kui Zhou, Man Liu, Xiaodong Guo, Yanzhen Qu, Wei Cui, ZengWu Shao, Xianglin Zhang, Shuyun Xu
Extrusion free-forming, as a rapid prototyping technique, is extensively applied in fabricating ceramic material in bone tissue engineering. To improve the osteoinductivity of nano-hydroxyapatite (nHA) scaffold fabricated by extrusion free-forming, in this study, we incorporated a new peptide (P28) and optimized the superficial microstructure after shaping by controlling the sintering temperature. P28, a novel bone morphogenic protein 2 (BMP-2)-related peptide, was designed in this study. Analysis of the structure, physicochemical properties, and release kinetics of P28 from nHA sintered at temperatures ranging from 1000 °C to 1400 °C revealed that nHA sintered at 1000 °C had higher porosity, preferable pore size, and better capacity to control P28 release than that sintered at other temperatures...
November 25, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27883153/organocatalyzed-asymmetric-michael-addition-by-an-efficient-bifunctional-carbohydrate-thiourea-hybrid-with-mechanistic-dft-analysis
#8
Chandra S Azad, Imran A Khan, Anudeep K Narula
A series of thiourea based bifunctional organocatalysts having d-glucose as a core scaffold were synthesized and examined as catalysts for the asymmetric Michael addition reaction of aryl/alkyl trans-β-nitrostyrenes over cyclohexanone and other Michael donors having active methylene. Excellent enantioselectivities (<95%), diastereoselectivities (<99%), and yields (<99%) were attained under solvent free conditions using 10 mol% of 1d0. The obtained results were explained through DFT calculations using the B3LYP/6-311G(d,p)//B3LYP/6-31G(d) basic set...
November 24, 2016: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/27881684/conformational-flexibility-of-an-anti-il-13-darpin%C3%A2
#9
Alexey Teplyakov, Thomas J Malia, Galina Obmolova, Steven A Jacobs, Karyn T O'Neil, Gary L Gilliland
Designed ankyrin repeat proteins (DARPin(®)) are artificial non-immunoglobulin binding proteins with potential applications as therapeutic molecules. DARPin 6G9 binds interleukin-13 with high affinity and blocks the signaling pathway and as such is promising for the treatment of asthma and other atopic diseases. The crystal structures of DARPin 6G9 in the unbound form and in complex with IL-13 were determined at high resolution. The DARPin competes for the same epitope as the IL-13 receptor chain 13Rα1 but does not interfere with the binding of the other receptor chain, IL-4Rα...
November 23, 2016: Protein Engineering, Design & Selection: PEDS
https://www.readbyqxmd.com/read/27881642/clinoptilolite-pcl-peg-pcl-composite-scaffolds-for-bone-tissue-engineering-applications
#10
Engin Pazarçeviren, Özge Erdemli, Dilek Keskin, Ayşen Tezcaner
The aim of this study was to prepare and characterize highly porous clinoptilolite/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) composite scaffolds. Scaffolds with different clinoptilolite contents (10% and 20%) were fabricated with reproducible solvent-free powder compression/particulate leaching technique. The scaffolds had interconnective porosity in the range of 55-76%. Clinoptilolite/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) scaffolds showed negligible degradation within eight weeks and displayed less water uptake and higher bioactivity than poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) scaffolds...
November 23, 2016: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/27881410/taz-activation-drives-fibroblast-spheroid-growth-expression-of-pro-fibrotic-paracrine-signals-and-context-dependent-ecm-gene-expression
#11
Amy J Jorgenson, Kyoung Moo Choi, Delphine Sicard, Karry M J Smith, Samantha E Hiemer, Xaralabos Varelas, Daniel J Tschumperlin
Recent studies have implicated the Hippo pathway and its transcriptional effectors YAP and TAZ as necessary for fibroblast activation and tissue fibrosis. To test the specific and sufficient roles for TAZ in driving autonomous fibroblast activation, we cultured NIH3T3 fibroblasts expressing a doxycycline-inducible nuclear-localized mutant of TAZ (TAZ4SA) in scaffold-free 3D hanging drop spheroids, or on matrices of specified mechanical rigidity. Control NIH3T3 fibroblasts formed spheroids in hanging drop culture that remained stable and neither increased nor decreased in size significantly over 15 days...
November 23, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27879223/generation-of-human-induced-pluripotent-stem-cells-in-defined-feeder-free-conditions
#12
Athena Kyrkou, Dimitris Stellas, Marika Syrrou, Apostolos Klinakis, Theodore Fotsis, Carol Murphy
Herein, we describe a modified protocol for the generation of human induced pluripotent stem cells (hiPS) and expansion under defined, serum free and feeder free conditions. These cells exhibit a high level of plasticity towards various differentiation pathways both in vitro and in vivo. Ultimately, hiPS-derived lines achieved high standards of three dimensional differentiations on biomaterial scaffolds and promoted in vivo regeneration of complex organs, such as Anterior Cruciate Ligament (in swine ACL-rupture models) and other tissues as well...
September 2016: Stem Cell Research
https://www.readbyqxmd.com/read/27878118/cancer-related-functions-and-subcellular-localizations-of-septins
#13
REVIEW
Christian Poüs, Laurence Klipfel, Anita Baillet
Since the initial discovery of septin family GTPases, the understanding of their molecular organization and cellular roles keeps being refined. Septins have been involved in many physiological processes and the misregulation of specific septin gene expression has been implicated in diverse human pathologies, including neurological disorders and cancer. In this minireview, we focus on the importance of the subunit composition and subcellular localization of septins relevant to tumor initiation, progression, and metastasis...
2016: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/27876851/the-genome-transcriptome-and-proteome-of-the-nematode-steinernema-carpocapsae-evolutionary-signatures-of-a-pathogenic-lifestyle
#14
Alejandra Rougon-Cardoso, Mitzi Flores-Ponce, Hilda Eréndira Ramos-Aboites, Christian Eduardo Martínez-Guerrero, You-Jin Hao, Luis Cunha, Jonathan Alejandro Rodríguez-Martínez, Cesaré Ovando-Vázquez, José Roberto Bermúdez-Barrientos, Cei Abreu-Goodger, Norberto Chavarría-Hernández, Nelson Simões, Rafael Montiel
The entomopathogenic nematode Steinernema carpocapsae has been widely used for the biological control of insect pests. It shares a symbiotic relationship with the bacterium Xenorhabdus nematophila, and is emerging as a genetic model to study symbiosis and pathogenesis. We obtained a high-quality draft of the nematode's genome comprising 84,613,633 bp in 347 scaffolds, with an N50 of 1.24 Mb. To improve annotation, we sequenced both short and long RNA and conducted shotgun proteomic analyses. S. carpocapsae shares orthologous genes with other parasitic nematodes that are absent in the free-living nematode C...
November 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27875633/design-synthesis-and-evaluation-of-dasatinib-amino-acid-and-dasatinib-fatty-acid-conjugates-as-protein-tyrosine-kinase-inhibitors
#15
Rakesh K Tiwari, Alex Brown, Neda Sadeghiani, Amir Nasrolahi Shirazi, Jared Bolton, Amanda Tse, Gennady Verkhivker, Keykavous Parang, Gongqin Sun
Derivatives of the tyrosine kinase inhibitor dasatinib were synthesized by esterification with 25 carboxylic acids, including amino acids and fatty acids, thereby extending the drug to interact with more diverse sites and to improve specificity. The dasatinib-l-arginine derivative (Das-R, 7) was found to be the most potent of the inhibitors tested, with IC50 values of 4.4, <0.25, and <0.45 nm against Csk, Src, and Abl kinases, respectively. The highest selectivity ratio obtained in our study, 91.4 Csk/Src, belonged to compound 18 (Das-C10 ) with an IC50 value of 3...
November 22, 2016: ChemMedChem
https://www.readbyqxmd.com/read/27870923/covering-the-implant-prosthesis-screw-access-hole-a-biological-approach-to-material-selection-and-technique
#16
Todd Schoenbaum, Chandur Wadhwani, Richard Stevenson
The traditional material for covering the implant abutment screw is cotton. As the implant-abutment junction flexes and wears overtime, leakage from the oral environment penetrates into the screw access chamber where the cotton pellet is located. The cotton serves as a scaffolding for the proliferation of anaerobic bacteria in the warm, oxygen free environment. The bacterial exudate causes foul odors and may cause additional inflammation of the peri-implant tissues. The use of PTFE tape or PVS impression material serves the same role as the cotton pellet, but forms a better seal and limits bacterial growth inside the implant and abutment...
November 21, 2016: Journal of Oral Implantology
https://www.readbyqxmd.com/read/27868204/bioengineering-pediatric-scaffold-free-auricular-cartilaginous-constructs
#17
Pedram Akbari, Stephen D Waldman, Sharon L Cushing, Blake C Papsin, Evan J Propst, Joanna F Weber, Herman Yeger, Walid A Farhat
OBJECTIVE: The use of exogenous materials as scaffolds in cartilage tissue engineering has limited the clinical application of resultant constructs due to the risk of postoperative complications. In an effort to minimize such complications, we aim to generate human, scaffold-free auricular cartilaginous constructs. STUDY DESIGN: Laboratory study using pediatric auricular cartilage. METHODS: Remnant, normal pediatric auricular cartilage samples that would have otherwise been discarded were collected and digested to free cells...
November 21, 2016: Laryngoscope
https://www.readbyqxmd.com/read/27866022/novel-approaches-toward-the-generation-of-bioscaffolds-as-a-potential-therapy-in-cardiovascular-tissue-engineering
#18
REVIEW
Fahimeh Shahabipour, Maciej Banach, Thomas P Johnston, Matteo Pirro, Amirhossein Sahebkar
Cardiovascular disease associated with myocardial infarction (MI) is among the leading causes of mortality worldwide, in part, due to the limited regenerative capacity of tissues. Although various approaches have been employed to generate bioartificial myocardial tissues, including surgical reconstruction and the use of biosynthetic or biological cell-free grafts, many challenges still remain. Natural biomaterials based on decellularization have made significant inroads into the development of favorable biomatrices for myocardial tissue regeneration...
November 9, 2016: International Journal of Cardiology
https://www.readbyqxmd.com/read/27863068/scaffold-free-tissue-engineering-of-functional-corneal-stromal-tissue
#19
Fatima N Syed-Picard, Yiqin Du, Andrew J Hertsenberg, Rachelle Palchesko, Martha L Funderburgh, Adam W Feinberg, James L Funderburgh
Blinding corneal scarring is predominately treated with allogeneic graft tissue, however, there is a worldwide shortage of donor tissue leaving millions in need of therapy. Human corneal stromal stem cells (CSSC) have been shown produce corneal tissue when cultured on nanofiber scaffolding, but this tissue cannot be readily separated from the scaffold. In this study, scaffold-free tissue engineering methods were used to generate biomimetic corneal stromal tissue constructs that can be transplanted in vivo without introducing the additional variables associated with exogenous scaffolding...
November 15, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27861930/scaffold-free-cartilage-tissue-engineering-with-a-small-population-of-human-nasoseptal-chondrocytes
#20
Loraine L Y Chiu, William T H To, John M Lee, Stephen D Waldman
OBJECTIVE: Cartilage tissue engineering is a promising approach to provide suitable materials for nasal reconstruction; however, it typically requires large numbers of cells. We have previously shown that a small number of chondrocytes cultivated within a continuous flow bioreactor can elicit substantial tissue growth, but translation to human chondrocytes is not trivial. Here, we aimed to demonstrate the application of the bioreactor to generate large-sized tissues from a small population of primary human nasoseptal chondrocytes...
November 15, 2016: Laryngoscope
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