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N-glycosylation

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https://www.readbyqxmd.com/read/29164392/maturation-and-cytokine-pattern-of-human-dendritic-cells-in-response-to-different-yeasts
#1
Silvia Boschi Bazan, Barbara Walch-Rückheim, Manfred J Schmitt, Frank Breinig
Activated dendritic cells (DC) induce and polarize T-cell responses by expression of distinct maturation markers and cytokines. This study systematically investigated the capacity of different biotechnically relevant yeast species and strains including Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis, Pichia pastoris, Hansenula polymorpha, Yarrowia lipolytica, and Candida glabrata to initiate maturation of human DC. As important prerequisite for T-cell activation, all yeasts were shown to effectively induce, though to a different extent, the expression of the activation marker CD83, the co-stimulatory molecules CD80, CD86, CD54, CD58, and CD40, as well as the antigen-presenting molecules MHCs I and II...
November 21, 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/29164281/monitoring-of-post-mortem-changes-of-saliva-n-glycosylation-by-nano-lc-ms
#2
Bum Jin Kim, Chanyoung Han, Hantae Moon, Joseph Kwon, Ik-Soon Jang, Si-Keun Lim, Ki-Won Park, Jong-Soon Choi, Hyun Joo An
The estimation of post-mortem interval (PMI) is a crucial part for investigations of crime and untimely deaths in forensic science. However, standard methods of PMI estimation are easily confounded by extenuating circumstances and/or environmental factors. Therefore, a panel of PMI markers obtained from a more acceptable and accurate method is necessary to definitely determine time of death. Saliva, one of the vital fluids encountered at crime scenes, contains various glycoproteins that are highly affected by biochemical environment...
November 21, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29162637/analysis-of-mammalian-o-glycopeptides-%C3%A2-we-have-made-a-good-start-but-there-is-a-long-way-to-go
#3
Zsuzsanna Darula, Katalin F Medzihradszky
Glycosylation is perhaps the most common post-translational modification. Recently there has been growing interest in cataloging the glycan structures, glycoproteins, and specific sites modified and deciphering the biological functions of glycosylation. While the results are piling up for N-glycosylation, O-glycosylation is seriously trailing behind. In our review we reiterate the difficulties researchers have to overcome in order to characterize O-glycosylation. We describe how an ingenious cell engineering method delivered exciting results, and also what could we gain from 'wild-type' samples...
November 21, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29157724/differential-n-glycan-patterns-identified-in-lung-adenocarcinoma-by-n-glycan-profiling-of-formalin-fixed-paraffin-embedded-ffpe-tissue-sections
#4
Xiaoning Wang, Zaian Deng, Chuncui Huang, Tong Zhu, Jiatao Lou, Lin Wang, Yan Li
N-glycan profiling is a powerful approach for analyzing the functional relationship between N-glycosylation and cancer. Current methods rely on either serum or fresh tissue samples; however, N-glycan patterns may differ between serum and tissue, as the proteins of serum originate from a variety of tissues. Furthermore, fresh tissue samples are difficult to ship and store. Here, we used a profiling method based on formalin-fixed paraffin-embedded (FFPE) tissue sections from lung adenocarcinoma patients. We found that our method was highly reproducible...
November 17, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29156678/epigenetic-silencing-of-tumor-suppressor-candidate-3-confers-adverse-prognosis-in-early-colorectal-cancer
#5
Elke Burgermeister, Patrick Höde, Johannes Betge, Tobias Gutting, Andreas Merkel, Wen Wu, Marc Tänzer, Maximilian Mossner, Daniel Nowak, Julia Magdeburg, Felix Rückert, Carsten Sticht, Katja Breitkopf-Heinlein, Nadine Schulte, Nicolai Härtel, Sebastian Belle, Stefan Post, Timo Gaiser, Barbara Ingold Heppner, Hans-Michael Behrens, Christoph Röcken, Matthias P A Ebert
Colorectal cancer (CRC) is a biologically and clinically heterogeneous disease. Even though many recurrent genomic alterations have been identified that may characterize distinct subgroups, their biological impact and clinical significance as prognostic indicators remain to be defined. The tumor suppressor candidate-3 (TUSC3/N33) locates to a genomic region frequently deleted or silenced in cancers. TUSC3 is a subunit of the oligosaccharyltransferase (OST) complex at the endoplasmic reticulum (ER) which catalyzes bulk N-glycosylation of membrane and secretory proteins...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29149631/prostaglandin-j2-promotes-o-glcnacylation-raising-app-processing-by-%C3%AE-and-%C3%AE-secretases-relevance-to-alzheimer-s-disease
#6
Teneka Jean-Louis, Patricia Rockwell, Maria E Figueiredo-Pereira
Regulation of the amyloid precursor protein (APP) processing by α- and β-secretases is of special interest to Alzheimer's disease (AD), as these proteases prevent or mediate amyloid beta formation, respectively. Neuroinflammation is also implicated in AD. Our data demonstrate that the endogenous mediator of inflammation prostaglandin J2 (PGJ2) promotes full-length APP (FL-APP) processing by α- and β-secretases. The decrease in FL-APP was independent of proteasomal, lysosomal, calpain, caspase, and γ-secretase activities...
November 14, 2017: Neurobiology of Aging
https://www.readbyqxmd.com/read/29146600/n-glycan-profile-and-kidney-disease-in-type-1-diabetes
#7
Mairead L Bermingham, Marco Colombo, Stuart J McGurnaghan, Luke A K Blackbourn, Frano Vučković, Maja Pučić Baković, Irena Trbojević-Akmačić, Gordan Lauc, Felix Agakov, Anna S Agakova, Caroline Hayward, Lucija Klarić, Colin N A Palmer, John R Petrie, John Chalmers, Andrew Collier, Fiona Green, Robert S Lindsay, Sandra Macrury, John A McKnight, Alan W Patrick, Sandeep Thekkepat, Olga Gornik, Paul M McKeigue, Helen M Colhoun
OBJECTIVE: Poorer glycemic control in type 1 diabetes may alter N-glycosylation patterns on circulating glycoproteins, and these alterations may be linked with diabetic kidney disease (DKD). We investigated associations between N-glycans and glycemic control and renal function in type 1 diabetes. RESEARCH DESIGN AND METHODS: Using serum samples from 818 adults who were considered to have extreme annual loss in estimated glomerular filtration rate (eGFR; i.e., slope) based on retrospective clinical records, from among 6,127 adults in the Scottish Diabetes Research Network Type 1 Bioresource Study, we measured total and IgG-specific N-glycan profiles...
November 16, 2017: Diabetes Care
https://www.readbyqxmd.com/read/29136858/monoclonal-antibody-n-glycosylation-profiling-using-capillary-electrophoresis-mass-spectrometry-assessment-and-method-validation
#8
Jérémie Giorgetti, Valentina D'Atri, Julie Canonge, Antony Lechner, Davy Guillarme, Olivier Colas, Elsa Wagner-Rousset, Alain Beck, Emmanuelle Leize-Wagner, Yannis-Nicolas François
Characterization of therapeutic proteins represents a major challenge for analytical sciences due to their heterogeneity caused by post-translational modifications (PTM). Among these PTM, glycosylation which is possibly the most prominent, require comprehensive identification because of their major influence on protein structure and effector functions of monoclonal antibodies (mAbs). As a consequence, glycosylation profiling must be deeply characterized. For this application, several analytical methods such as separation-based or MS-based methods, were evaluated...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29133251/immune-response-of-interferon-%C3%AE-inducible-lysosomal-thiol-reductase-gilt-from-chinese-sturgeon-acipenser-sinensis-to-microbial-invasion-and-its-antioxdative-activity-in-lipopolysaccharides-treated-mammalian-dentritic-cells
#9
Xiuling You, Liu Liu, Xiuyu Li, Hejun Du, Dongsong Nie, Xingguo Zhang, Haibing Tong, Mingjiang Wu, Yitian Gao, Zhiyong Liao
Interferon-γ-inducible lysosomal thiol reductase (GILT) plays an important role in the major histocompatibility complex-restricted antigen processing of endocytosed proteins via catalyzing the disulfide bond reduction in the endocytic pathway. Here, the cDNA of Chinese sturgeon (Acipenser sinensis) GILT (CsGILT) was cloned. It contained an open reading frame of 762 nucleotides encoding a protein of 254 amino acids with an estimated molecular weight of 28.1 kDa. The characteristic structural features, including a signature sequence CQHGX2ECX2NX4C, a CXXC motif, two potential N-glycosylation sites, and eight conserved cysteines were detected in the deduced amino acid sequence of CsGILT...
November 10, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29121057/trafficking-and-processing-of-bacterial-proteins-by-mammalian-cells-insights-from-chondroitinase-abc
#10
Elizabeth Muir, Mansoor Raza, Clare Ellis, Emily Burnside, Fiona Love, Simon Heller, Matthew Elliot, Esther Daniell, Debayan Dasgupta, Nuno Alves, Priscilla Day, James Fawcett, Roger Keynes
BACKGROUND: There is very little reported in the literature about the relationship between modifications of bacterial proteins and their secretion by mammalian cells that synthesize them. We previously reported that the secretion of the bacterial enzyme Chondroitinase ABC by mammalian cells requires the strategic removal of at least three N-glycosylation sites. The aim of this study was to determine if it is possible to enhance the efficacy of the enzyme as a treatment for spinal cord injury by increasing the quantity of enzyme secreted or by altering its cellular location...
2017: PloS One
https://www.readbyqxmd.com/read/29119999/ion-mobility-resolved-collision-induced-dissociation-and-electron-transfer-dissociation-of-n-glycopeptides-gathering-orthogonal-connectivity-information-from-a-single-mass-selected-precursor-ion-population
#11
Venkata Kolli, Katherine N Schumacher, Eric D Dodds
Glycopeptide-level mass spectrometry (MS) and tandem mass spectrometry (MS/MS) analyses are commonly performed to establish site-specific protein glycosylation profiles that are of central importance to gaining structure-function insights on glycoproteins. Confoundingly, the complete characterization of glycopeptide connectivity usually requires the acquisition of multiple MS/MS fragmentation spectra. Complementary ion fragmentation techniques such as collision-induced dissociation (CID) and electron transfer dissociation (ETD) are often applied in concert to address this need...
November 9, 2017: Analyst
https://www.readbyqxmd.com/read/29119347/n-glycan-structures-and-downstream-mannose-phosphorylation-of-plant-recombinant-human-alpha-l-iduronidase-toward-development-of-enzyme-replacement-therapy-for-mucopolysaccharidosis-i
#12
Owen M Pierce, Grant R McNair, Xu He, Hiroyuki Kajiura, Kazuhito Fujiyama, Allison R Kermode
Arabidopsis N-glycan processing mutants provide the basis for tailoring recombinant enzymes for use as replacement therapeutics to treat lysosomal storage diseases, including N-glycan mannose phosphorylation to ensure lysosomal trafficking and efficacy. Functional recombinant human alpha-L-iduronidase (IDUA; EC 3.2.1.76) enzymes were generated in seeds of the Arabidopsis thaliana complex-glycan-deficient (cgl) C5 background, which is deficient in the activity of N-acetylglucosaminyl transferase I, and in seeds of the Arabidopsis gm1 mutant, which lacks Golgi α-mannosidase I (GM1) activity...
November 8, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/29113801/producing-a-glycosylating-escherichia-coli-cell-factory-the-placement-of-the-bacterial-oligosaccharyl-transferase-pglb-onto-the-genome
#13
Benjamin Strutton, Stephen R P Jaffé, Jagroop Pandhal, Phillip C Wright
Although Escherichia coli has been engineered to perform N-glycosylation of recombinant proteins, an optimal glycosylating strain has not been created. By inserting a codon optimised Campylobacter oligosaccharyltransferase onto the E. coli chromosome, we created a glycoprotein platform strain, where the target glycoprotein, sugar synthesis and glycosyltransferase enzymes, can be inserted using expression vectors to produce the desired homogenous glycoform. To assess the functionality and glycoprotein producing capacity of the chromosomally based OST, a combined Western blot and parallel reaction monitoring mass spectrometry approach was applied, with absolute quantification of glycoprotein...
November 4, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29111354/three-gpi-anchored-alkaline-phosphatases-are-involved-in-the-intoxication-of-cry1ca-toxin-to-spodoptera-exigua-larvae
#14
Xiang-Liang Ren, Hong-Yan Hu, Wei-Li Jiang, Xiao-Yan Ma, Ya-Jie Ma, Guo-Qing Li, Yan Ma
Glycosylphosphatidylinositol (GPI)-anchored alkaline phosphatase (ALP) isoforms are crucial for the intoxication of crystal proteins (Cry) to several insect species. We cloned five SeALPs from the larval midgut of Spodoptera exigua, a major pest of many crops. All five SeALPs contain a signal peptide at the N-terminus, a phosphatase domain, and a GPI-anchor site at the C-terminus. Additionally, the sequences encode two or three potential N-glycosylation sites. The five SeALPs were highly expressed at the larval stage, especially in the larval gut or Malpighian tubules...
October 27, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/29110469/comparative-analysis-of-whey-n-glycoproteins-in-human-colostrum-and-mature-milk-using-quantitative-glycoproteomics
#15
Xueyan Cao, Dahe Song, Mei Yang, Ning Yang, Qing Ye, Dongbing Tao, Biao Liu, Rina Wu, Xiqing Yue
Glycosylation is a ubiquitous post-translational protein modification that plays a substantial role in various processes. However, whey glycoproteins in human milk have not been completely profiled. Herein, we used quantitative glycoproteomics to quantify whey N-glycosylation sites and their alteration in human milk during lactation; 110 N-glycosylation sites on 63 proteins and 91 N-glycosylation sites on 53 proteins were quantified in colostrum and mature milk whey, respectively. Among these, 68 glycosylation sites on 38 proteins were differentially expressed in human colostrum and mature milk whey...
November 14, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29109265/diminished-ost3-dependent-n-glycosylation-of-the-bip-nucleotide-exchange-factor-sil1-is-an-adaptive-response-to-reductive-er-stress
#16
Kofi L P Stevens, Amy L Black, Kelsi M Wells, K Y Benjamin Yeo, Robert F L Steuart, Colin J Stirling, Benjamin L Schulz, Carl J Mousley
BiP (Kar2 in yeast) is an essential Hsp70 chaperone and master regulator of endoplasmic reticulum (ER) function. BiP's activity is regulated by its intrinsic ATPase activity that can be stimulated by two different nucleotide exchange factors, Sil1 and Lhs1. Both Sil1 and Lhs1 are glycoproteins, but how N-glycosylation regulates their function is not known. Here, we show that N-glycosylation of Sil1, but not of Lhs1, is diminished upon reductive stress. N-glycosylation of Sil1 is predominantly Ost3-dependent and requires a functional Ost3 CxxC thioredoxin motif...
November 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29106622/a-light-sensitive-mutation-in-arabidopsis-lew3-reveals-the-important-role-of-n-glycosylation-in-root-growth-and-development
#17
Concepción Manzano, Mercedes Pallero-Baena, J Silva-Navas, Sara Navarro Neila, Ilda Casimiro, Pedro Casero, Jose M Garcia-Mina, Roberto Baigorri, Lourdes Rubio, Jose A Fernandez, Matthew Norris, Yiliang Ding, Miguel A Moreno-Risueno, Juan C Del Pozo
Plant roots have the potential capacity to grow almost indefinitely if meristematic and lateral branching is sustained. In a genetic screen we identified an Arabidopsis mutant showing limited root growth (lrg1) due to defects in cell division and elongation in the root meristem. Positional cloning determined that lrg1 affects an alpha-1,2-mannosyltransferase gene, LEW3, involved in protein N-glycosylation. The lrg1 mutation causes a synonymous substitution that alters the correct splicing of the fourth intron in LEW3, causing a mix of wild-type and truncated protein...
November 2, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29101027/identification-and-characterization-of-udp-mannose-in-human-cell-lines-and-mouse-organs-differential-distribution-across-brain-regions-and-organs
#18
Kazuki Nakajima, Yasuhiko Kizuka, Yoshiki Yamaguchi, Yoshio Hirabayashi, Kazuo Takahashi, Yukio Yuzawa, Naoyuki Taniguchi
Mannosylation in the endoplasmic reticulum is a key process for synthesizing various glycans. Guanosine diphosphate mannose (GDP-Man) and dolichol phosphate-mannose serve as donor substrates for mannosylation in mammals and are used in N-glycosylation, O-mannosylation, C-mannosylation, and the synthesis of glycosylphosphatidylinositol-anchor (GPI-anchor). Here, we report for the first time that low-abundant uridine diphosphate-mannose (UDP-Man), which can serve as potential donor substrate, exists in mammals...
October 31, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29100363/alterations-in-expressed-prostate-secretion-urine-psa-n-glycosylation-discriminate-prostate-cancer-from-benign-prostate-hyperplasia
#19
Gaozhen Jia, Zhenyang Dong, Chenxia Sun, Fuping Wen, Haifeng Wang, Huaizu Guo, Xu Gao, Chuanliang Xu, Chuanliang Xu, Chenghua Yang, Yinghao Sun
The prostate specific antigen (PSA) test is widely used for early diagnosis of prostate cancer (PCa). However, its limited sensitivity has led to over-diagnosis and over-treatment of PCa. Glycosylation alteration is a common phenomenon in cancer development. Different PSA glycan subforms have been proposed as diagnostic markers to better differentiate PCa from benign prostate hyperplasia (BPH). In this study, we purified PSA from expressed prostate secretions (EPS)-urine samples from 32 BPH and 30 PCa patients and provided detailed PSA glycan profiles in Chinese population...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29089960/proline-hydroxylation-in-cell-wall-proteins-is-it-yet-possible-to-define-rules
#20
Harold Duruflé, Vincent Hervé, Thierry Balliau, Michel Zivy, Christophe Dunand, Elisabeth Jamet
Cell wall proteins (CWPs) play critical and dynamic roles in plant cell walls by contributing to developmental processes and response to environmental cues. Since the CWPs go through the secretion pathway, most of them undergo post-translational modifications (PTMs) which can modify their biological activity. Glycosylation is one of the major PTMs of CWPs and refers to N-glycosylation, O-glycosylation and glypiation. Each of these PTMs occurs in different amino acid contexts which are not all well defined. This article deals with the hydroxylation of Pro residues which is a prerequisite for O-glycosylation of CWPs on hydroxyproline (Hyp) residues...
2017: Frontiers in Plant Science
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