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senstivity and specificity

Senem Yaman Karadam, Sema Ertuğ, Hatice Ertabaklar
OBJECTIVE: The aim of this study was to compare direct microscopic examination, direct fluorescent antibody assay (DFA), and the immunochromatographic method (IK) and identify the best suitable method for the diagnosis of Giardia intestinalis. METHODS: In this study, 25 stool samples that had been diagnosed as being infected with G. intestinalis using the native-Lugol and/or formol-ethyl acetate concentration method and 25 non-parasite-infected samples (the control group) were examined...
March 2016: Türkiye Parazitolojii Dergisi
R Nagi, Y-B Reddy-Kantharaj, N Rakesh, S Janardhan-Reddy, S Sahu
BACKGROUND: Earlier detection of oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMD) is essential for dental professionals to improve patient survival rates. The aim of this systematic review is to to evaluate the effectiveness of devices that utilise the principles of chemiluminescence and tissue autofluorescence as adjuncts in the detection of OSCC and OPMD. MATERIAL AND METHODS: The electronic retrieval systems and databases searched for relevant articles were PubMed [MEDLINE] and Science direct...
2016: Medicina Oral, Patología Oral y Cirugía Bucal
Hang J Lee, Yoongul Oh, KyeHee Cho, MinYoung Kim, Hee-kyu Kwon
PURPOSE: This study is to determine the diagnostic utility of optimal recording electrode placement for distal latency comparison of median-second lumbrical and ulnar-interossei/third lumbrical (M2L-UI3L) in carpal tunnel syndrome. METHODS: Sixty-five hands of control and 75 hands of 62 clinically suspected carpal tunnel syndromes were used for the M2L-UI3L and standard conduction studies. To obtain optimal M2L-UI3L, the recording active electrode (E1) was placed at the midpalm over the third metacarpal bone, whereas the reference electrode (E2) was attached to the palmar digital crease area...
April 2016: Journal of Clinical Neurophysiology: Official Publication of the American Electroencephalographic Society
Fengjiao He, Yunchang Xiong, Jing Liu, Feifei Tong, Danyang Yan
Au-IDE/CFP10-ESAT6 aptamer/DNA-AuNPs MSPQC for rapid detection of Mycobacterium tuberculosis was constructed based on specific detection of specific fused antigen CFP10-ESAT6 which secreted only by pathogenic M. tuberculosis in its early culture time. CFP10-ESAT6 aptamer was used as sensor specific probe of CFP10-ESAT6 antigen. Au nanoparticles (NPs) was employed to increase sensor senstivity. The Au-IDE/CFP10-ESAT6 aptamer/DNA-AuNPs electrode probe was prepared by modifying of the complementary DNA-AuNPs on to interdigital array microelectrode with CFP10-ESAT6 aptamer...
March 15, 2016: Biosensors & Bioelectronics
Vinod Kumar, Munish Shorie, Ashok K Ganguli, Priyanka Sabherwal
We report a label free electrochemical detection of cardiac bio-marker myoglobin (Mb) on aptamer functionalized rGO/CNT nanostructured electrodes by measuring its direct electron transfer (DET). Configured as a highly responsive aptasensor, the newly developed biosensing platform exhibits synergistic effect of the nano-hybrid functional construct by combining good electrical properties and the facile chemical functionality of nanohybrid for the compatible bio-interface development. The specific anti-Mb aptamer was generated by five iterative SELEX (Systematic evolution of ligands by exponential enrichment) rounds, showing high senstivity (KD ~65 pM)...
October 15, 2015: Biosensors & Bioelectronics
Martin D Witte, Tongfei Wu, Carla P Guimaraes, Christopher S Theile, Annet E M Blom, Jessica R Ingram, Zeyang Li, Lenka Kundrat, Shalom D Goldberg, Hidde L Ploegh
Transpeptidation catalyzed by sortase A allows the preparation of proteins that are site-specifically and homogeneously modified with a wide variety of functional groups, such as fluorophores, PEG moieties, lipids, glycans, bio-orthogonal reactive groups and affinity handles. This protocol describes immobilization of sortase A on a solid support (Sepharose beads). Immobilization of sortase A simplifies downstream purification of a protein of interest after labeling of its N or C terminus. Smaller batch and larger-scale continuous-flow reactions require only a limited amount of enzyme...
March 2015: Nature Protocols
Devi Dayal, Hemant Jain, Savita Verma Attri, Bhavneet Bharti, Anil Kumar Bhalla
CONTEXT: High senstivity C-reactive protein (hsCRP) levels correlate well other parameters of obesity related metabolic syndrome (MS) and can be used as predictors of future cardiovascular disease risk. There is limited data on hsCRP levels in Indian children with simple obesity. AIM: To study the relationship of hsCRP levels with various anthropometric as well as metabolic parameters in children with simple overweight and obesity. MATERIALS AND METHODS: This case control study was conducted in Paediatric Endocrinology clinic of a tertiary care hospital in Northern India...
August 2014: Journal of Clinical and Diagnostic Research: JCDR
Bineeta Kashyap, Namita Srivastava, Iqbal R Kaur, Rajat Jhamb, Deepak K Singh
BACKGROUND: Tuberculosis (TB) is an increasing public health concern worldwide. On a global scale it has a devastating impact in developing nations. Genital TB, an extrapulmonary form, is not uncommon particularly in areas where pulmonary TB is prevalent. Genital TB may be asymptomatic or may even masquerade as other gynaecological conditions; hence, diagnosis requires a high degree of suspicion and the use of appropriate investigations. OBJECTIVE: This study attempted to identify endometrial TB in endometrial biopsies taken from women evaluated for infertility by comparison of various staining techniques...
July 2013: Iranian Journal of Reproductive Medicine
Changwei Luo, Zachary Y Huang, Kun Li, Xiaoming Chen, You Chen, Yongyu Sun
The Eastern honey bee, [Apis cerana (F.)], is an important and common pollinator for an important biodiesel tree, [Jatropha curcas (L.)]. To understand sensitivity of A. cerana to different floral compounds, we quantified volatile floral compounds of J. curcas, then determined electroantennogram (EAG) responses of A. cerana to 11 compounds each at five doses (0.4, 4, 40, 400, and 4,000 microg) of six most active floral compounds. Our results demonstrated that floral compounds of J. curcas differ in variety and quantity while linalool is always a major constituent in floral blends from three different plantations...
August 2013: Journal of Economic Entomology
Mikhail A Kutuzov, Alexandra V Andreeva, Nelly Bennett
Rhodopsin kinase (GRK1) is a member of G protein-coupled receptor kinase family and a key enzyme in the quenching of photolysed rhodopsin activity and desensitisation of the rod photoreceptor neurons. Like some other rod proteins involved in phototransduction, GRK1 is posttranslationally modified at the C terminus by isoprenylation (farnesylation), endoproteolysis and α-carboxymethylation. In this study, we examined the potential mechanisms of regulation of GRK1 methylation status, which have remained unexplored so far...
December 2012: Cellular Signalling
Yajuan Wang, Marc Simon, Pramod Bonde, Bronwyn U Harris, Jeffrey J Teuteberg, Robert L Kormos, James F Antaki
Right ventricular failure is a significant complication following implantation of a left ventricular assist device (LVAD), which increases morbidity and mortality. Consequently, researchers have sought predictors that may identify patients at risk. However, they have lacked sensitivity and/or specificity. This study investigated the use of a decision tree technology to explore the preoperative data space for combinatorial relationships that may be more accurate and precise. We retrospectively analyzed the records of 183 patients with initial LVAD implantation at the Artificial Heart Program, University of Pittsburgh Medical Center, between May 1996 and October 2009...
May 2012: IEEE Transactions on Information Technology in Biomedicine
Juhi Upadhyay, Nirmala N Rao, Ram B Upadhyay, Pankaj Agarwal
Being simple and inexpensive toluidine blue has been in use for more than two decades for the detection of potentially malignant oral lesions (PMOL's) and malignant lesions. Although there has been concensus that staining often assists in the identification of these lesions, results have been diverse. In most studies false negative were not recorded as biopsies of lesions that did not retain toluidine blue were not performed. Thus the present study attempted to evaluate the efficacy of toluidine blue vital dye for detection of PMOL's...
2011: Asian Pacific Journal of Cancer Prevention: APJCP
Lina Chen, Jianuo Liu, Changshui Xu, James Keblesh, Weijin Zang, Huangui Xiong
Human immunodeficiency virus type 1 (HIV-1)-associated dementia (HAD) usually occurs late in the course of HIV-1 infection and the mechanisms underlying HAD pathogenesis are not well understood. Accumulating evidence indicates that neuronal voltage-gated potassium (Kv) channels play an important role in memory processes and acquired neuronal channelopathies in HAD. To examine whether Kv channels are involved in HIV-1-associated neuronal injury, we studied the effects of HIV-1 glycoprotein 120 (gp120) on outward K+ currents in rat cortical neuronal cultures using whole-cell patch techniques...
2011: PloS One
Sudhakar Morankar, Ayalew Tegene, Wondwossen Kassahun, Sultan Sulueiman, Yihenew Alem Negatu, Meseret Yazachew, Franco Pagnoni
BACKGROUND: Malaria shows variations in its epidemiologic pattern in relation to the local situation. Rapid diagnostic tests (RDTs) have the potential to provide an accurate diagnosis and to determine the prevalence of malaria in ureas. OBJECTIVE: The purpose of the study was to determine the prevalence of malaria and to evaluate the sensitivity and specificity of RDT for diagnosis of malaria. METHODS: A health facility based cross-sectional study was conducted from September to November 2006 among Jebrile children of less than five years of age as part of interventional study to develop and test home management of malaria in Jimma Town...
April 2011: Ethiopian Medical Journal
P Marín Reina, I Ruiz Alcántara, S Vidal Micó, J L López-Prats Lucea, V Modesto I Alapont
OBJECTIVE: To evaluate the diagnostic accuracy of serum procalcitonin (PCT) to detect severe bacterial infection (SBI) in ambulatory children attended in the emergency room (ER) for fever without source (FWS). MATERIAL AND METHODS: A search was made in MEDLINE, OVID and EMBASE (to January 2010). We searched for papers that evaluated the diagnostic accuracy of serum PCT to detect SBI in children that, being previously well, were seen in the ER for FWS. We rated the methodological quality of each paper using objective validity criteria (QUADAS, CASPE) and included only those with the maximum quality in the analysis...
June 2010: Anales de Pediatría: Publicación Oficial de la Asociación Española de Pediatría (A.E.P.)
Andrei L Kindzelskii, Howard R Petty
We have tested Galvanovskis and Sandblom's prediction that ion channel clustering enhances weak electric field detection by cells as well as how the elicited signals couple to metabolic alterations. Electric field application was timed to coincide with certain known intracellular chemical oscillators (phase-matched conditions). Polarized, but not spherical, neutrophils labeled with anti-K(v)1.3, FL-DHP, and anti-TRP1, but not anti-T-type Ca(2+) channels, displayed clusters at the lamellipodium. Resonance energy transfer experiments showed that these channel pairs were in close proximity...
December 2005: European Biophysics Journal: EBJ
Tulay Koken, Orhan C Aktepe, Mustafa Serteser, Murat Samli, Ahmet Kahraman, Nurhan Dogan
Leucocytes and bacteria in urine are the important indices for diagnosing urinary tract infections. Urine specimens from 260 patients were examined for leucocytes and bacteria with the UF-100 flow cytometer (Sysmex Corporation, Japan). The results obtained with the UF-100 were compared with those obtained by bacteriologic cultures of the same urine samples. Bacteriologic samples were found to be positive in 48 urine samples. The best detected cut-off value for bacteria is 3800/microL (senstivity = 73%, specificity = 78%) and 25/microL for leucocytes (sensitivity = 73%, specificity = 92%)...
2002: International Urology and Nephrology
Daoyu Hu, Chengyuan Wang, M Langer
To elevate the clinical value of 3D-FLASH in the diagnosis of anal fistula and compare it with convertioned MR sequence, MR sequences, consisting of spin echo T1WI, turbo invertion recovery magnitude (TIRM), fast low-angle shot image (FLASH), mon-enhancement and enhancement substraction and coronary reconstructing, was conducted in 15 cases suspected of anal fistula. Comparison was made among the three sequences in display rate of internal fistula, external fistula, the branch of fistule connulas. Our results showed that 1 patient had perianal abscess...
2002: Journal of Huazhong University of Science and Technology. Medical Sciences
U A Germann, P J Ford, D Shlyakhter, V S Mason, M W Harding
Overexpression of the multidrug resistance MDR1 gene product P-glycoprotein and/or the multidrug resistance-associated protein MRP confers multidrug resistance to cancer cells. The pipecolinate derivative VX-710 has previously been demonstrated to reverse MDR1-mediated multidrug resistance at concentrations of 0.5-2.5 microM by direct interaction with P-glycoprotein and inhibition of its drug efflux activity. In this study we investigated whether VX-710 as well as four other known MDR1 modulators could also reverse multidrug resistance mediated by MRP...
February 1997: Anti-cancer Drugs
H Kirchhoff, J Heitmann, W Bisping
Mycoplasmas were isolated from 11 (=#6,8%) of 161 cervix swabs from infertile mares. A total of 17 strains was isolated and characterized by indirect immunofluorescent test and metabolic inhibition test as Mycoplasma equigenitalium (11 strains), Mycoplasma subdolum (2 strains), Acholeplasma laidlawii (3 strains) and Acholeplasma equifetale (1 strain). In addition cervix swabs of the mares were investigated for bacteria. There was no specific correlation between presence of mycoplasmas and bacteria (table 1)...
March 1980: Zentralblatt Für Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie
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