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Epstein JI

Hee Jae Huh, Jong Eun Park, Ji Youn Kim, Sun Ae Yun, Myoung Keun Lee, Nam Yong Lee, Jong Won Kim, Chang Seok Ki
There has been increasing interest in standardized and quantitative Epstein-Barr virus (EBV) DNA testing for the management of EBV disease. We evaluated the performance of the Real-Q EBV Quantification Kit (BioSewoom, Korea) in whole blood (WB). Nucleic acid extraction and real-time PCR were performed by using the MagNA Pure 96 (Roche Diagnostics, Germany) and 7500 Fast real-time PCR system (Applied Biosystems, USA), respectively. Assay sensitivity, linearity, and conversion factor were determined by using the World Health Organization international standard diluted in EBV-negative WB...
March 2017: Annals of Laboratory Medicine
Hanah Kim, Mina Hur, Ji Young Kim, Hee Won Moon, Yeo Min Yun, Hyun Chan Cho
BACKGROUND: Cytomegalovirus (CMV) and Epstein-Barr virus (EBV) are increasingly important in immunocompromised patients. Nucleic acid extraction methods could affect the results of viral nucleic acid amplification tests. We compared two automated nucleic acid extraction systems for detecting CMV and EBV using real-time PCR assays. METHODS: One hundred and fifty-three whole blood (WB) samples were tested for CMV detection, and 117 WB samples were tested for EBV detection...
March 2017: Annals of Laboratory Medicine
Ji Mok Kim, Chun Woo Song, Kyu Sang Song, Jae Young Kim
Infectious mononucleosis is Epstein-Barr virus (EBV) inducing a self-limiting clinical syndrome characterized by fever, sore throat, hepatosplenomegaly, and generalized lymphadenopathy. Gastrointestinal symptoms of EBV infection are nonspecific and occur rarely. EBV inducing acute gastrointestinal pathology is poorly recognized without suspicion. Careful consideration is needed to diagnose gastric involvement of EBV infection including gastric lymphoma, gastric cancer, and gastritis. A few recent cases of gastritis associated with EBV infection have been reported in adolescents and adults...
November 2016: Korean Journal of Pediatrics
Tao Huang, Yan Ji, Dan Hu, Baozheng Chen, Hejun Zhang, Chao Li, Gang Chen, Xingguang Luo, Xiong-Wei Zheng, Xiandong Lin
The Epstein-Barr virus (EBV) is associated with a variety of cancers, including gastric cancer, which has one of the highest mortality rates of all human cancers. Long non-coding RNAs (lncRNAs) have been suggested to have important causal roles in gastric cancer. However, the interaction between lncRNAs and EBV has not yet been studied. To this end, we sequenced 11,311 lncRNAs and 144,826 protein-coding transcripts from four types of tissue: one non-EBV-infected gastric carcinoma (EBVnGC) and its adjacent normal tissue, and one EBV-associated gastric carcinoma (EBVaGC) and its adjacent normal tissue...
November 7, 2016: Oncotarget
Cheol Keun Park, Ji Soo Park, Hyo Song Kim, Sun Young Rha, Woo Jin Hyung, Jae-Ho Cheong, Sung Hoon Noh, Sang Kil Lee, Yong Chan Lee, Yong-Min Huh, Hyunki Kim
Although targeted therapy for receptor tyrosine kinases (RTKs) of advanced gastric cancers (AGCs) has been in the spotlight, guidelines for the identification of RTK-amplified gastric cancers (RA-GCs) have not been established. In this study, we investigate clinicopathologic characteristics of RA-GCs and propose a screening algorithm for their identification. We performed immunohistochemistry (IHC) for MLH1, MSH2, PMS2, MSH6, key RTKs (EGFR, HER2, MET), and p53, in situ hybridization for Epstein-Barr virus encoding RNA, and silver in situ hybridization (SISH) for EGFR, HER2, and MET using tissue microarrays of 993 AGCs...
September 27, 2016: Oncotarget
Nathan D Montgomery, Wilborn B Coward, Steven Johnson, Ji Yuan, Margaret L Gulley, Stephanie P Mathews, Kathleen Kaiser-Rogers, Kathleen W Rao, Warren G Sanger, Jennifer N Sanmann, Yuri Fedoriw
Classical Hodgkin lymphoma (CHL) is morphologically characterized by scattered malignant Hodgkin/Reed-Sternberg (HRS) cells that are far outnumbered by surrounding reactive hematolymphoid cells. Approximately half of all cases of CHL are associated with infection by Epstein-Barr virus (EBV), an oncogenic herpesvirus that expresses a number of proteins thought to contribute to transformation. While a small number of published studies have attempted to identify recurrent cytogenetic abnormalities in CHL, no large case series have explored karyotypic differences between EBV-positive and EBV-negative tumors...
September 2016: Cancer Genetics
Lin Ren, Weibing She, Qingyu Gao, Changwei Pan, Chen Ji, Irving R Epstein
Crawling motion mediated by retrograde and direct waves, that is, in the opposite or the same direction, respectively, as the muscular wave that generates it, is a fundamental mode of biological locomotion, from which more complex and sophisticated locomotion modes involving outgrowths such as limbs and wings may have evolved. A detailed general description of muscular wave locomotion and its relationship with other modes of locomotion is a challenging task. We employ a model of a photosensitive self-oscillating gel, in which chemical pulse waves and a stimulus-responsive medium play roles analogous to nerve pulses and deformable muscles in an animal, to generate retrograde and direct waves under non-uniform illumination...
October 13, 2016: Angewandte Chemie
Sun Jiji, Zhao Xiaoxu, Qiao Lihua, Mei Shuang, Nie Zhipeng, Zhang Qinghai, Ji Yanchun, Jiang Pingping, Guan Min-Xin
Mitochondrial DNA (mtDNA) mutations cause a variety of mitochondrial DNA-based diseases which have been studied using Lymphoblastoid cell lines (LCLs) and transmitochondrial cybrids. Individual genetic information is preserved permanently in LCLs while the development of transmitochondrial cybrids provide ex-vivo cellular platform to study molecular mechanism of mitochondrial DNA-based diseases. The cytoplasmic donor cells for previous transmitochondrial cybrids come from patient's tissue or platelet directly...
July 20, 2016: Yi Chuan, Hereditas
Xin-Mao Song, Zhu-Jian Wang, Wen-Jun Cao, Ji-Li, Fu Chen, Sheng-Zi Wang
BACKGROUND: Early diagnosis of nasopharyngeal carcinoma (NPC) needs more reliable biomarkers. The aim of this study was to investigate serum cytokeratin 19 fragment 21.1 (CYFRA21-1) as an NPC biomarker based on data from a large sample. METHODS: From October 2010 to February 2014, 529 subjects were enrolled and divided into three groups-NPC group (n = 274), healthy control group (n = 175) and nasal inflammatory disease group (n = 80). Serum CYFRA21-1 levels were measured prior to radiotherapy/chemoradiotherapy, and their associations with T, N, and clinical classification were determined...
December 2016: International Journal of Clinical Oncology
Hyo Jeong Kang, In-Seob Lee, Young-Soo Park, Won Jin Ho, DaHye Sohn, Ji-Yong Ahn, Jeong-Hwan Yook, Byung-Sik Kim
BACKGROUND: Epstein-Barr virus-positive gastric carcinoma (EBVGC) constitutes approximately 10 % of all gastric carcinoma cases. While distinct molecular features have been characterized previously, there have not been studies identifying their clinical utility. The purpose of this study was to investigate the immunohistochemistry (IHC) profile of EBVGC and to evaluate the potential clinicopathologic and prognostic significance of each marker. METHODS: Clinicopathologic characteristics of 234 patients (203 males and 31 females) who underwent curative gastrectomy for EBVGCs from 1998 to 2013 at Asan Medical Center, were reviewed, and IHC for ARID1A, PTEN, PD-L1, p53, p16(INK4a), MLH1, and MSH2 were performed on tissue microarrays...
October 2016: Annals of Surgical Oncology
Zuojuan Zhang, Juandong Wang, Buqiang Ji, Tatiana von Bahr Greenwood, Yuan Zhang, Yongjing Wang, Dexiao Kong, Ai Li, Yang Jiang, Yanan Guo, Xiaoli Liu, Yingxue Wang, Aixia Dou, Nailin Li, Jan-Inge Henter, Guizhen Sun, Chengyun Zheng
OBJECTIVE: Hemophagocytic lymphohistiocytosis in adults is largely underdiagnosed. To improve the rate and accuracy of diagnosis in adults, the clinical and laboratory characteristics of hemophagocytic lymphohistiocytosis were analyzed in and compared between adults and children in a Chinese cohort. METHOD: Data from 50 hemophagocytic lymphohistiocytosis patients, including 34 adults and 16 children who fulfilled the 2004 hemophagocytic lymphohistiocytosis diagnostic criteria, were collected and analyzed...
April 2016: Clinics
Jin Ho Paik, Ji-Young Choe, Hyojin Kim, Jeong-Ok Lee, Hyoung Jin Kang, Hee Young Shin, Dong Soon Lee, Dae Seog Heo, Chul-Woo Kim, Kwang-Hyun Cho, Tae Min Kim, Yoon Kyung Jeon
Epstein-Barr virus-positive T/NK-cell lymphoproliferative diseases (EBV-T/NK-LPDs) include several overlapping EBV-related conditions with variably aggressive courses. For prognostic categorization, we retrospectively analyzed 42 EBV-T/NK-LPD cases. Male (79% [33/42]), young (≤40 years; 83% [35/42]) patients and T-cell lineage (81% [34/42]; CD8/CD4 = 1.8) were predominant. Clinicopathologically, three systemic and one cutaneous category were developed: hemophagocytic lymphohistiocytosis (HLH; 26% [11/42]), chronic active EBV infection (CAEBV; 31% [13/42]), systemic unclassifiable disease (24% [10/42]), and hydroa vacciniforme/hydroa vacciniforme-like lymphoma (HV/HVL; 19% [8/42])...
January 2017: Leukemia & Lymphoma
Woo Jin Lee, Hyun Ji Kang, Chong Hyun Won, Sung Eun Chang, Jee Ho Choi, Mi Woo Lee
Few studies have evaluated the histopathological features of cutaneous extranodal NK/T-cell lymphoma (ENKTL). The authors' study aims to describe cases of cutaneous ENKTL that histopathologically mimic benign inflammatory dermatosis. The authors searched the database of Asan Medical Center to identify cases of cutaneous ENKTL that were confirmed by skin biopsy between January 1998 and December 2014. The authors included cases that were initially diagnosed as benign inflammatory disorders on the histopathological examination, but finally diagnosed as cutaneous ENKTL on further evaluation...
May 3, 2016: American Journal of Dermatopathology
Wei Dai, Hong Zheng, Arthur Kwok Leung Cheung, Clara Sze-man Tang, Josephine Mun Yee Ko, Bonnie Wing Yan Wong, Merrin Man Long Leong, Pak Chung Sham, Florence Cheung, Dora Lai-Wan Kwong, Roger Kai Cheong Ngan, Wai Tong Ng, Chun Chung Yau, Jianji Pan, Xun Peng, Stewart Tung, Zengfeng Zhang, Mingfang Ji, Alan Kwok-Shing Chiang, Anne Wing-Mui Lee, Victor Ho-Fun Lee, Ka-On Lam, Kwok Hung Au, Hoi Ching Cheng, Harry Ho-Yin Yiu, Maria Li Lung
Multiple factors, including host genetics, environmental factors, and Epstein-Barr virus (EBV) infection, contribute to nasopharyngeal carcinoma (NPC) development. To identify genetic susceptibility genes for NPC, a whole-exome sequencing (WES) study was performed in 161 NPC cases and 895 controls of Southern Chinese descent. The gene-based burden test discovered an association between macrophage-stimulating 1 receptor (MST1R) and NPC. We identified 13 independent cases carrying the MST1R pathogenic heterozygous germ-line variants, and 53...
March 22, 2016: Proceedings of the National Academy of Sciences of the United States of America
Ji Hyun Park, Dok Hyun Yoon, Shin Kim, Jung Sun Park, Chan-Sik Park, Heungsup Sung, Sang-Wook Lee, Jooryung Huh, Cheolwon Suh
Epstein-Barr virus (EBV) in the peripheral blood has become a significant predictor of clinical outcomes in EBV-associated Hodgkin lymphoma (HL). However, due to its relative rarity, prevalence and prognostic role of circulating EBV-DNA has not been well established in Asian patients. Seventy patients with newly diagnosed HL were prospectively registered between October 2007 and January 2013, and underwent pretreatment whole blood (WB) EBV-DNA quantitation using real-time polymerase chain reaction (RT-PCR)...
April 2016: Annals of Hematology
Hai-Juan Xiao, Ji Li, Hong-Mei Song, Zheng-Hong Li, Mei Dong, Xiao-Ge Zhou
Systemic Epstein-Barr virus (EBV)-positive T-cell lymphoproliferative disorders (LPDs) of childhood is a highly aggressive EBV-positive T/natural killer (NK)-cell LPD, which emerges in the background of chronic active EBV infection (CAEBV) or shortly after primary acute EBV infection. The clinical presentations of CAEBV are varied; patients with atypical manifestations are easily misdiagnosed. We described a 14-year-old boy suffering from digestive disorders and intermittent fever for 1 year and 9 months, whose conditions worsened and skin lesions occurred 2 months before hospitalization...
February 2016: Medicine (Baltimore)
Ying Liu, Wenjun Yang, Yaqi Pan, Jiafu Ji, Zheming Lu, Yang Ke
Epstein-Barr virus (EBV) is linked to the development of a variety of malignancies, including EBV-associated gastric carcinoma (EBVaGC). In this study, EBVaGC was detected in 15 (7.3%) of 206 GC cases. To identify the EBV genomic variation, EBV genomic sequences isolated from 9 EBVaGC biopsy specimens were successfully retrieved, designated EBVaGC1 to EBVaGC9. By comparative analysis of these strains with another 6 completely sequenced EBV strains, EBV-wild type, B95-8, AG876, GD1, GD2, and HKNPC1, it was demonstrated that EBVaGC1 to 9 were most closely related to the GD1 strain...
January 26, 2016: Oncotarget
Ji-Qing Chen, Erika Zilahi, Gábor Papp, Sándor Sipka, Margit Zeher
AIM: The microRNA-155 (miR-155) is regarded as a central modulator of T-cell responses and could be a potential therapeutic target for certain inflammatory diseases. In our present study we analyzed the expression rate of miR-155 and its functionally linked gene, the suppressor gene of cytokine signaling 1 (SOCS1) in primary Sjögren's syndrome (pSS). METHOD: We enrolled 23 pSS patients and 10 healthy individuals in the study. The expression of miR-155 and SOCS1 gene were measured by real-time polymerase chain reaction...
December 1, 2015: International Journal of Rheumatic Diseases
Guo-Fang Guan, De-Jun Zhang, Lian-Ji Wen, Duo-Jiao Yu, Yan Zhao, Lin Zhu, Ying-Yuan Guo, Ying Zheng
BACKGROUND: There is increasing evidence demonstrating the role of human trophoblast cell surface antigen 2 (TROP2) in cancer development and progression. However, their prognostic value in Epstein-Barr virus (EBV) associated nasopharyngeal carcinoma (NPC) remains to be elucidated. METHOD: The prognostic significances of TROP2 and Ki-67 were determined by immunohistochemistry in 58 NPC samples. TROP2 mRNA expression level and biological functions were evaluated...
2015: International Journal of Clinical and Experimental Pathology
Xiaofang Bian, Garrick Wallstrom, Amy Davis, Jie Wang, Jin Park, Andrea Throop, Jason Steel, Xiaobo Yu, Clive Wasserfall, Desmond Schatz, Mark Atkinson, Ji Qiu, Joshua LaBaer
The rapid rise in the incidence of type 1 diabetes (T1D) suggests the involvement of environmental factors including viral infections. We evaluated the association between viral infections and T1D by profiling antiviral antibodies using a high-throughput immunoproteomics approach in patients with new-onset T1D. We constructed a viral protein array comprising the complete proteomes of seven viruses associated with T1D and open reading frames from other common viruses. Antibody responses to 646 viral antigens were assessed in 42 patients with T1D and 42 age- and sex-matched healthy control subjects (mean age 12...
January 2016: Diabetes
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