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Listeria screening

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https://www.readbyqxmd.com/read/29145803/unraveling-the-evolution-and-coevolution-of-small-regulatory-rnas-and-coding-genes-in-listeria
#1
Franck Cerutti, Ludovic Mallet, Anaïs Painset, Claire Hoede, Annick Moisan, Christophe Bécavin, Mélodie Duval, Olivier Dussurget, Pascale Cossart, Christine Gaspin, Hélène Chiapello
BACKGROUND: Small regulatory RNAs (sRNAs) are widely found in bacteria and play key roles in many important physiological and adaptation processes. Studying their evolution and screening for events of coevolution with other genomic features is a powerful way to better understand their origin and assess a common functional or adaptive relationship between them. However, evolution and coevolution of sRNAs with coding genes have been sparsely investigated in bacterial pathogens. RESULTS: We designed a robust and generic phylogenomics approach that detects correlated evolution between sRNAs and protein-coding genes using their observed and inferred patterns of presence-absence in a set of annotated genomes...
November 16, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29105633/evaluation-of-antimicrobial-cytotoxic-and-genotoxic-activities-of-ganoderma-lucidum-reishi-mushroom
#2
Bulent Ergun
Ganoderma lucidum (GL) is a mushroom used as a traditional remedy for the treatment of various infections since ancient times. This study, was aimed to investigate antimicrobial activity potential of GL against Staphylococcus aureus, Enterococcus faecalis, Listeria monocytogenes, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, Candida albicans, Candida glabrata, Candida krusei, and Candida parapsilopsis. Furthermore, it was also aimed to evaluate the toxicity potential of GL. Antimicrobial activities were screened by using microbroth dilution method...
September 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29029084/penicillin-binding-protein-encoded-by-pbp4-is-involved-in-mediating-copper-stress-in-listeria-monocytogenes
#3
Cameron Parsons, Ben Costolo, Phillip Brown, Sophia Kathariou
Listeria monocytogenes raises major food safety and public health concerns due its potential for severe foodborne disease and persistent colonization of food processing facilities. Copper is often employed to control pathogens in agriculture and is increasingly used in healthcare facilities, but mechanisms mediating tolerance of L. monocytogenes to copper remain poorly understood. A mariner-based mutant library of L. monocytogenes 2011L-2858, implicated in the 2011 listeriosis outbreak via whole cantaloupe, was screened for growth at sublethal levels of copper yielding mutant G2B4 with decreased copper tolerance...
October 4, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28990669/effect-of-thyme-oil-alginate-based-coating-on-quality-and-microbial-safety-of-fresh-cut-apples
#4
Sarengaowa, Wenzhong Hu, Aili Jiang, Zhilong Xiu, Ke Feng
BACKGROUND: Food preservation is critical for keeping fresh-cut products fresh, nutritious, safe, attractive and available for consumers. To improve the safety and quality of fresh-cut fruits, fifteen essential oils (EOs) were screened to test the antimicrobial activity against Listeria monocytogenes (LM), Salmonella typhimurium (ST), Staphylococcus aureus (SA) and Escherichia coli O157:H7 (EC O157:H7). The effect of alginate-based edible coating (EC) incorporating with different concentrations thyme oil on fresh-cut Red Fuji apples was investigated...
October 9, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28990290/synthesis-characterization-antimicrobial-activity-and-docking-studies-of-new-triazolic-tripodal-ligands
#5
Tarik Harit, Reda Bellaouchi, Yahya Rokni, Abdelkhalek Riahi, Fouad Malek, Abdeslam Asehraou
The synthesis and characterization of new N-donor bitriazolic tripods were reported. The in vitro antibacterial and antifungal activities of these products were screened against fungal strain (Candida pelliculosa) and against four bacterial strains (Micrococcus luteus, Bacillus subtilis, Listeria innocua and Escherichia coli). Biological data revealed the effect of the chemical structure on antimicrobial activity. Molecular docking studies of some compounds showed that they could act as inhibitors for the biotin carboxylase enzyme...
October 9, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28974981/docking-study-synthesis-and-antimicrobial-evaluation-of-some-novel-4-anilinoquinazoline-derivatives
#6
Rezvan Rezaee Nasab, Farshid Hassanzadeh, Ghadam Ali Khodarahmi, Mahboubeh Rostami, Mahmoud Mirzaei, Ali Jahanian-Najafabadi, Mahboubeh Mansourian
A series of novel 4-anilinoquinazoline derivatives were designed and synthesized from benzoic acid through ring closure, chlorination or nucleophilic substitution. The structures of compounds were characterized by IR, (1)H-NMR and mass spectroscopy. All synthesized derivatives were screened for their antimicrobial activities against Gram-positive (Staphylococcus aurous, Bacillus subtilis, Listeria monocitogenes) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Salmonella entritidis) bacteria and also for antifungal activities against Candida albicans using the conventional micro dilution method...
October 2017: Research in Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28957011/prevalence-of-trichomonas-salmonella-and-listeria-in-wild-birds-from-southeast-texas
#7
Britni Brobey, Ashwini Kucknoor, Jim Armacost
Infectious diseases can be a major threat to wildlife populations, especially in human-modified habitats, but infection rates in populations of wild animals are often poorly studied. Trichomonas, Salmonella, and Listeria are all pathogens known to infect birds, but their infection rates in wild bird populations are not well documented. This study documents infection rates of the three pathogens in wild bird populations inhabiting a suburban to rural gradient in Southeast Texas. Various species of wild birds were sampled at five sites in Southeastern Texas representing rural (<1 house per ha), exurban (approximately 1 house per ha), and suburban (approximately 10 houses per ha) habitat types...
September 2017: Avian Diseases
https://www.readbyqxmd.com/read/28910141/rapid-screening-of-natural-plant-extracts-with-calcium-diacetate-for-differential-effects-against-foodborne-pathogens-and-a-probiotic-bacterium
#8
William Colonna, Byron Brehm-Stecher, Kalidas Shetty, Anthony Pometto Iii
This study focused on advancing a rapid turbidimetric bioassay to screen antimicrobials using specific cocktails of targeted foodborne bacterial pathogens. Specifically, to show the relevance of this rapid screening tool, the antimicrobial potential of generally recognized as safe calcium diacetate (DAX) and blends with cranberry (NC) and oregano (OX) natural extracts was evaluated. Furthermore, the same extracts were evaluated against beneficial lactic acid bacteria. The targeted foodborne pathogens evaluated were Escherichia coli O157:H7, Salmonella spp...
September 14, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28887418/chitinase-expression-in-listeria-monocytogenes-is-influenced-by-lmo0327-which-encodes-an-internalin-like-protein
#9
Dafni Katerina Paspaliari, Vicky Gaedt Kastbjerg, Hanne Ingmer, Magdalena Popowska, Marianne Halberg Larsen
The chitinolytic system of Listeria monocytogenes thus far comprises two chitinases, ChiA and ChiB, and a lytic polysaccharide monooxygenase, Lmo2467. The role of the system in the bacterium appears to be pleiotropic, as besides mediating the hydrolysis of chitin, the second most ubiquitous carbohydrate in nature, the chitinases have been deemed important for the colonization of unicellular molds, as well as mammalian hosts. To identify additional components of the chitinolytic system, we screened a transposon mutant library for mutants exhibiting impaired chitin hydrolysis...
November 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28843123/antimicrobial-efficacy-of-cinnamomum-javanicum-plant-extract-against-listeria-monocytogenes-and-its-application-potential-with-smoked-salmon
#10
Wenqian Yuan, Hui Wen Lee, Hyun-Gyun Yuk
Extracts from medicinal plants have been reported to possess good antimicrobial properties, but a majority of them remain unexplored. This study aimed at identifying a novel plant extract with antimicrobial activity, to validate its efficacy in food model, and to elucidate its composition and antimicrobial mechanism. A total of 125 plant extracts were screened, and Cinnamomum javanicum leaf and stem extract showed potential antimicrobial activity against Listeria monocytogenes (MIC=0.13mg/mL). Total phenolic content of the extract was 78...
August 19, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28827366/spontaneous-loss-of-virulence-in-natural-populations-of-listeria-monocytogenes
#11
Mylène M Maury, Viviane Chenal-Francisque, Hélène Bracq-Dieye, Lei Han, Alexandre Leclercq, Guillaume Vales, Alexandra Moura, Edith Gouin, Mariela Scortti, Olivier Disson, José A Vázquez-Boland, Marc Lecuit
The pathogenesis of Listeria monocytogenes depends on the ability of this bacterium to escape from the phagosome of the host cells via the action of the pore-forming toxin listeriolysin O (LLO). Expression of the LLO-encoding gene (hly) requires the transcriptional activator PrfA, and both hly and prfA genes are essential for L. monocytogenes virulence. Here, we used the hemolytic activity of LLO as a phenotypic marker to screen for spontaneous virulence-attenuating mutations in L. monocytogenes Sixty nonhemolytic isolates were identified among a collection of 57,820 confirmed L...
November 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28825544/crystal-diagnostics-xpress-%C3%A2-lm-kit-for-the-rapid-detection-of-listeria-monocytogenes-from-environmental-surfaces
#12
Curtis H Stumpf, Brian Bullard, Weidong Zhoa, Stephanie Kuzenko-Hentosh, Gary D Niehaus
The Crystal Diagnostics (CDx) Xpress™ LM kit is used for rapid screening of low concentrations of Listeria monocytogenes on environmental surfaces such as stainless steel, plastic, and ceramic tile. In addition to the Xpress LM kit, the CDx Xpress System comprises an automatic Xpress Reader, a BioCassette™ that incorporates antibody-coupled microspheres, and liquid crystal for selective identification of the intended microbe. All 56 of the 56 tested L. monocytogenes strains evaluated were detected, and 50 of the 50 nontarget bacterial strains were excluded when the test was conducted under the described kit conditions...
January 1, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28821610/a-screen-for-kinase-inhibitors-identifies-antimicrobial-imidazopyridine-aminofurazans-as-specific-inhibitors-of-the-listeria-monocytogenes-pasta-kinase-prka
#13
Adam J Schaenzer, Nathan Wlodarchak, David H Drewry, William J Zuercher, Warren E Rose, Rob Striker, John-Demian Sauer
Bacterial signaling systems such as protein kinases and quorum sensing have become increasingly attractive targets for the development of novel antimicrobial agents in a time of rising antibiotic resistance. The family of bacterial Penicillin-binding-protein And Serine/Threonine kinase-Associated (PASTA) kinases is of particular interest due to the role of these kinases in regulating resistance to β-lactam antibiotics. As such, small-molecule kinase inhibitors that target PASTA kinases may prove beneficial as treatments adjunctive to β-lactam therapy...
October 13, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28803008/two-step-large-volume-magnetic-separation-combined-with-pcr-assay-for-sensitive-detection-of-listeria-monocytogenes-in-pasteurized-milk
#14
Dan Luo, Xiaolin Huang, Yan Mao, Chaochao Chen, Fulai Li, Hengyi Xu, Yonghua Xiong
Immunomagnetic separation (IMS) is an effective tool for the preconcentration and purification of food-borne pathogens from complex food samples because of its high capture efficiency (CE). In conventional IMS, antibodies are usually conjugated on the surface of magnetic beads (MB); the random orientation and conformation changes of antibodies on the MB surface can decrease their bioactivity. Moreover, the Brownian motion of immobilized antibodies is weakened, thereby rendering their binding efficiency with bacteria lower than that of free antibodies...
August 9, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28798957/holographic-deep-learning-for-rapid-optical-screening-of-anthrax-spores
#15
YoungJu Jo, Sangjin Park, JaeHwang Jung, Jonghee Yoon, Hosung Joo, Min-Hyeok Kim, Suk-Jo Kang, Myung Chul Choi, Sang Yup Lee, YongKeun Park
Establishing early warning systems for anthrax attacks is crucial in biodefense. Despite numerous studies for decades, the limited sensitivity of conventional biochemical methods essentially requires preprocessing steps and thus has limitations to be used in realistic settings of biological warfare. We present an optical method for rapid and label-free screening of Bacillus anthracis spores through the synergistic application of holographic microscopy and deep learning. A deep convolutional neural network is designed to classify holographic images of unlabeled living cells...
August 2017: Science Advances
https://www.readbyqxmd.com/read/28757183/rapid-screening-and-identification-of-living-pathogenic-organisms-via-optimized-bioorthogonal-non-canonical-amino-acid-tagging
#16
Allison Rae Sherratt, Yanouchka Rouleau, Christian Luebbert, Miroslava Strmiskova, Teodor Veres, Sabah Bidawid, Nathalie Corneau, John Paul Pezacki
Pathogenic bacteria can be a major cause of illness from environmental sources as well as the consumption of contaminated products, giving rise to public health concerns globally. The surveillance of such living organisms in food and water supplies remains an important challenge in mitigating their deleterious societal effects. Here, we have developed an optimized bioorthogonal non-canonical amino acid tagging approach to the imaging, capture, and interrogation of shigatoxigenic/verotoxigenic Escherichia coli (VTEC) and Listeria that enables the distinction between living wild-type pathogenic bacteria...
August 17, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28744832/isolation-and-selection-of-potential-probiotic-bacteria-from-the-pig-gastrointestinal-tract
#17
Luca Lo Verso, Martin Lessard, Guylaine Talbot, Benoit Fernandez, Ismail Fliss
The present study aimed to isolate bacterial strains from the pig gastrointestinal tract that have antagonistic activity against potential pathogens and are able to produce antimicrobial compounds. That ability would be a first requirement for the strains' possible use as probiotics. Samples obtained from pig intestinal mucosa and contents were screened for the presence of antagonistic activity against pathogenic indicator strains of Escherichia coli, Salmonella, and Listeria by means of the double-layer technique...
July 25, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28719838/identification-of-a-prototypical-single-stranded-uracil-dna-glycosylase-from-listeria-innocua
#18
Jing Li, Ye Yang, Jose Guevara, Liangjiang Wang, Weiguo Cao
A recent phylogenetic study on UDG superfamily estimated a new clade of family 3 enzymes (SMUG1-like), which shares a lower homology with canonic SMUG1 enzymes. The enzymatic properties of the newly found putative DNA glycosylase are unknown. To test the potential UDG activity and evaluate phylogenetic classification, we isolated one SMUG1-like glycosylase representative from Listeria innocua (Lin). A biochemical screening of DNA glycosylase activity in vitro indicates that Lin SMUG1-like glycosylase is a single-strand selective uracil DNA glycosylase...
September 2017: DNA Repair
https://www.readbyqxmd.com/read/28703625/characterization-and-application-of-antilisterial-enterocins-on-model-fresh-cheese
#19
Susana C Ribeiro, R Paul Ross, Catherine Stanton, Célia C G Silva
Enterococcus faecalis strains isolated from an artisanal cheese were selected based on enterocin production against Listeria monocytogenes. The strains formed biofilms and presented high hydrophobic character and good autoaggregation and coaggregation capacity with L. monocytogenes. Strains L3A21M3 and L3B1K3 presented high survival under gastrointestinal conditions, were able to adhere to human intestinal cells (Caco-2 and HT-29), and blocked the adhesion and invasion of L. monocytogenes. The antilisterial activity of enterocins was not affected by pH (2 to 12), heating (100°C), and chemical and surfactant agents...
August 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28692184/traditional-empiric-antibiotic-treatment-is-still-effective-for-neonatal-fever
#20
Maytal Kristal, Vika Dor, Daniel Glikman
No abstract text is available yet for this article.
November 2017: Acta Paediatrica
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