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Listeria screening

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https://www.readbyqxmd.com/read/28803008/two-step-large-volume-magnetic-separation-combined-with-pcr-assay-for-sensitive-detection-of-listeria-monocytogenes-in-pasteurized-milk
#1
Dan Luo, Xiaolin Huang, Yan Mao, Chaochao Chen, Fulai Li, Hengyi Xu, Yonghua Xiong
Immunomagnetic separation (IMS) is an effective tool for the preconcentration and purification of food-borne pathogens from complex food samples because of its high capture efficiency (CE). In conventional IMS, antibodies are usually conjugated on the surface of magnetic beads (MB); the random orientation and conformation changes of antibodies on the MB surface can decrease their bioactivity. Moreover, the Brownian motion of immobilized antibodies is weakened, thereby rendering their binding efficiency with bacteria lower than that of free antibodies...
August 9, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28798957/holographic-deep-learning-for-rapid-optical-screening-of-anthrax-spores
#2
YoungJu Jo, Sangjin Park, JaeHwang Jung, Jonghee Yoon, Hosung Joo, Min-Hyeok Kim, Suk-Jo Kang, Myung Chul Choi, Sang Yup Lee, YongKeun Park
Establishing early warning systems for anthrax attacks is crucial in biodefense. Despite numerous studies for decades, the limited sensitivity of conventional biochemical methods essentially requires preprocessing steps and thus has limitations to be used in realistic settings of biological warfare. We present an optical method for rapid and label-free screening of Bacillus anthracis spores through the synergistic application of holographic microscopy and deep learning. A deep convolutional neural network is designed to classify holographic images of unlabeled living cells...
August 2017: Science Advances
https://www.readbyqxmd.com/read/28757183/rapid-screening-and-identification-of-living-pathogenic-organisms-via-optimized-bioorthogonal-non-canonical-amino-acid-tagging
#3
Allison Rae Sherratt, Yanouchka Rouleau, Christian Luebbert, Miroslava Strmiskova, Teodor Veres, Sabah Bidawid, Nathalie Corneau, John Paul Pezacki
Pathogenic bacteria can be a major cause of illness from environmental sources as well as the consumption of contaminated products, giving rise to public health concerns globally. The surveillance of such living organisms in food and water supplies remains an important challenge in mitigating their deleterious societal effects. Here, we have developed an optimized bioorthogonal non-canonical amino acid tagging approach to the imaging, capture, and interrogation of shigatoxigenic/verotoxigenic Escherichia coli (VTEC) and Listeria that enables the distinction between living wild-type pathogenic bacteria...
July 17, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28744832/isolation-and-selection-of-potential-probiotic-bacteria-from-the-pig-gastrointestinal-tract
#4
Luca Lo Verso, Martin Lessard, Guylaine Talbot, Benoit Fernandez, Ismail Fliss
The present study aimed to isolate bacterial strains from the pig gastrointestinal tract that have antagonistic activity against potential pathogens and are able to produce antimicrobial compounds. That ability would be a first requirement for the strains' possible use as probiotics. Samples obtained from pig intestinal mucosa and contents were screened for the presence of antagonistic activity against pathogenic indicator strains of Escherichia coli, Salmonella, and Listeria by means of the double-layer technique...
July 25, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28719838/identification-of-a-prototypical-single-stranded-uracil-dna-glycosylase-from-listeria-innocua
#5
Jing Li, Ye Yang, Jose Guevara, Liangjiang Wang, Weiguo Cao
A recent phylogenetic study on UDG superfamily estimated a new clade of family 3 enzymes (SMUG1-like), which shares a lower homology with canonic SMUG1 enzymes. The enzymatic properties of the newly found putative DNA glycosylase are unknown. To test the potential UDG activity and evaluate phylogenetic classification, we isolated one SMUG1-like glycosylase representative from Listeria innocua (Lin). A biochemical screening of DNA glycosylase activity in vitro indicates that Lin SMUG1-like glycosylase is a single-strand selective uracil DNA glycosylase...
July 8, 2017: DNA Repair
https://www.readbyqxmd.com/read/28703625/characterization-and-application-of-antilisterial-enterocins-on-model-fresh-cheese
#6
Susana C Ribeiro, R Paul Ross, Catherine Stanton, Célia C G Silva
Enterococcus faecalis strains isolated from an artisanal cheese were selected based on enterocin production against Listeria monocytogenes. The strains formed biofilms and presented high hydrophobic character and good autoaggregation and coaggregation capacity with L. monocytogenes. Strains L3A21M3 and L3B1K3 presented high survival under gastrointestinal conditions, were able to adhere to human intestinal cells (Caco-2 and HT-29), and blocked the adhesion and invasion of L. monocytogenes. The antilisterial activity of enterocins was not affected by pH (2 to 12), heating (100°C), and chemical and surfactant agents...
July 13, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28692184/traditional-empiric-antibiotic-treatment-is-still-effective-for-neonatal-fever
#7
Maytal Kristal, Vika Dor, Daniel Glikman
Fever may be the only sign of a serious bacterial infection (SBI) in neonates. Ampicillin plus cefotaxime (A+C) and ampicillin plus gentamicin (A+G) are commonly used as empiric therapies for Escherichia coli, Group B Streptococcus (GBS) and Listeria monocytogenes, which are the most likely neonatal SBI pathogens (1). Culture-based screening and prophylaxis has significantly lowered GBS infections and an alarming increase in the antimicrobial resistance rates of Gram-negative bacteria, including cefotaxime and gentamicin resistance, has been reported (1-3)...
July 10, 2017: Acta Paediatrica
https://www.readbyqxmd.com/read/28690383/molecular-docking-based-screening-of-listeriolysin-o-for-improved-inhibitors
#8
Sara Ghafari, Matin Komeilian, Mohaddese Sadat Hashemi, Sareh Oushani, Garshasb Rigi, Behnam Rashidieh, Kamran Yarahmadi, Fatemeh Khoddam
Listeriolysine-O (LLO) is a 50KDa protein responsible for Listeria monocytogenes pathogenicity. The structure of LLO (PDB ID: 4CDB) with domains D1 to D4 is known. Therefore, it is of interest to identify conserved regions among LLO variants for destabilizing oligomerization (50 mer complex) of its monomers using appropriate inhibitors. Therefore, it is of interest to identify suitable inhibitors for inhibiting LLO. Previous reports suggest the use of flavanoids like compounds for inhibiting LLO. Our interest is to identify improved compounds to destabilize LLO oligomerization...
2017: Bioinformation
https://www.readbyqxmd.com/read/28671588/implications-of-neuroinvasive-bacterial-peptides-on-rodents-behaviour-and-neurotransmission
#9
Aneela Taj, Nusrat Jamil
Neuroinvasive microbes are capable of applying their influences on the autonomic nervous system (ANS) of the host followed by the involvement of central nervous system (CNS) by releasing extracellular metabolites that may cause alterations in the biochemical and neurophysiological environment. Consequently synaptic, neuroendocrine, peripheral immune, neuro-immune, and behavioural responses of the host facilitate the progression of infection. The present study was designed to extrapolate the effects of crude and purified extracellular peptides of neuropathogenic bacteria on behavioural responses and neurotransmission of Sprague Dawley (SD) models...
July 2, 2017: Pathogens
https://www.readbyqxmd.com/read/28620364/a-multiplex-rt-pcr-assay-for-s-aureus-l-monocytogenes-and-salmonella-spp-detection-in-raw-milk-with-pre-enrichment
#10
Tian Ding, Yuanjie Suo, Zhaohuan Zhang, Donghong Liu, Xingqian Ye, Shiguo Chen, Yong Zhao
This study firstly developed a multiplex real-time PCR (RT-PCR) technique combined with a pre-enrichment step to simultaneously detect Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes) and Salmonella spp. in raw milk and the dairy farm environment (feces, soil, feed, water) in one reaction. Brain heart infusion (BHI) broth was selected for the enrichment step to increase the density of the target bacteria by using an incubation of 4 h before multiplex RT-PCR. The results showed that the detection limit of the multiplex real-time assay was approximately 10(2) CFU/mL for pure cultures and artificially contaminated milk without enrichment, while 12, 14, and 10 CFU/25 mL, respectively, for S...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28605978/isolation-and-preliminary-characterization-of-a-bacteriocin-producer-bacillus-strain-inhibiting-methicillin-resistant-staphylococcus-aureus
#11
Ankit Kumar Chauhan, Dinesh Kumar Maheshwari, Vivek K Bajpai
In a multivalent approach to discover new antimicrobial substance, a total of 160 Bacilli were isolated from termitarium soil, characterized on the basis of their morphological and physiological characters and screened for their antimicrobial activity by agar well diffusion method against certain drug resistant pathogenic bacteria such as Staphylococcus aureus, Methicillin resistant Staphylococcus aureus and common food contaminating bacteria Listeria monocytogenes. After preliminary screening, sixteen isolates showed inhibitory activity against test pathogens...
June 2017: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28598105/-genetic-recombiniation-and-protein-expression-detection-of-listeria-based-tuberculosis-vaccine-candidates
#12
Chen-Yan Ren, Si-Jing Liu, Qi-Kang Pu, Ming-Juan Jiang, Meng-Ying Zhou, Chuan Wang
OBJECTIVES: Genetic construction of tuberculosis vaccine candidates based on Listeria(L.) monocytogenes,L.ivanovii,and evaluation their protein expression,in order to provide a novel method for research on tuberculosis controlling. METHODS: Two kinds of gene cassettes carrying tuberculosis antigen encoding gene Rv3875 or Rv0129c were inserted into targeting vector harboring L.monocytogenes,L.ivanovii homologous sequences via genetic connection methods and plasmid transformation technology in vitro...
November 2016: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28583150/prevalence-of-pregnancy-relevant-infections-in-a-rural-setting-of-ghana
#13
Fabian Völker, Paul Cooper, Oliver Bader, Angela Uy, Ortrud Zimmermann, Raimond Lugert, Uwe Groß
BACKGROUND: Although infectious diseases still account for a high burden of morbidity and mortality in sub-Saharan Africa, simultaneous investigations on multiple infections affecting maternal and child health are missing. METHODS: We conducted a cross-sectional, single-centre pilot study in a rural area of Ghana to assess the infectiological profile during pregnancy. Screening of 180 expectant mothers was done by vaginal swabs and serology to detect the most common pregnancy-relevant infections...
June 6, 2017: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/28572793/bacteriocin-producing-enterococcus-faecium-lcw-44-a-high-potential-probiotic-candidate-from-raw-camel-milk
#14
Allison Vimont, Benoît Fernandez, Riadh Hammami, Ahlem Ababsa, Hocine Daba, Ismaïl Fliss
Bacterial isolates from raw camel milk were screened for antibacterial activity using the agar diffusion assay. Ten isolates selected for their inhibition of Gram-positive bacteria were identified by 16S sequencing as Enterococcus faecium or durans. An isolate named E. faecium LCW 44 exhibited the broadest antibacterial spectrum with an inhibitory activity against several Gram-positive strains belonging to the genera Clostridium, Listeria, Staphylococcus, and Lactobacillus. E. faecium LCW 44 was shown to produce N-formylated enterocins L50A and L50B, as revealed by mass spectrometry and PCR analyses...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28515653/assessment-of-microbial-and-radioactive-contaminations-in-korean-cold-duck-meats-and-electron-beam-application-for-quality-improvement
#15
Kyung-A An, Yunhee Jo, Muhammad Sajid Arshad, Gui-Ran Kim, Cheorun Jo, Joong-Ho Kwon
Animal-origin food products pose serious threat to public food safety due to high microbial loads. The microbial and radioactive contaminations in commercial cold duck meat products were evaluated. Ten different lots of commercial samples (C1-C10) were classified based on type and smoking process. All samples were highly contaminated (< 4-7 Log CFU/g) with total aerobic bacteria (TAB), yeasts and molds (Y&M), and 7 samples (C1-C7) were positive for coliforms. Furthermore, three samples were contaminated with Listeria monocytogenes (C4-C6) and one with Salmonella typhimurium (C6)...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/28451288/insights-into-clpxp-proteolysis-heterooligomerization-and-partial-deactivation-enhance-chaperone-affinity-and-substrate-turnover-in-listeria-monocytogenes
#16
Dóra Balogh, Maria Dahmen, Matthias Stahl, Marcin Poreba, Malte Gersch, Marcin Drag, Stephan A Sieber
Caseinolytic proteases (ClpP) are important for recognition and controlled degradation of damaged proteins. While the majority of bacterial organisms utilize only a single ClpP, Listeria monocytogenes expresses two isoforms (LmClpP1 and LmClpP2). LmClpPs assemble into either a LmClpP2 homocomplex or a LmClpP1/2 heterooligomeric complex. The heterocomplex in association with the chaperone ClpX, exhibits a boost in proteolytic activity for unknown reasons. Here, we use a combined chemical and biochemical strategy to unravel two activation principles of LmClpPs...
February 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28418717/diversity-of-endophytic-bacteria-in-a-fern-species-dryopteris-uniformis-makino-makino-and-evaluation-of-their-antibacterial-potential-against-five-foodborne-pathogenic-bacteria
#17
Gitishree Das, Seonjoo Park, Kwang-Hyun Baek
The fern plant Dryopteris uniformis has traditionally been used in herbal medicine and possesses many biological activities. This study was conducted to explore the endophytic bacterial diversity associated with D. uniformis and evaluate their antibacterial potential against foodborne pathogenic bacteria (FPB). Among 51 isolated endophytic bacteria (EB), 26 EB were selected based on their morphological characteristics and identified by 16S rRNA gene analysis. The distribution of EB was diverse in the leaf and the stem/root tissues...
May 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28411150/comparative-diagnostic-efficacy-of-recombinant-llo-and-pi-plc-based-elisas-for-detection-of-listeriosis-in-animals
#18
COMPARATIVE STUDY
Rahul D Suryawanshi, Satya Veer Singh Malik, Bhushan Jayarao, Sandeep P Chaudhari, Emily Savage, Jess Vergis, Nitin V Kurkure, Sukhadeo B Barbuddhe, Deepak B Rawool
The present study for the first time evaluates the serodiagnostic efficacy of two recombinant antigens namely, listeriolysin O (rLLO) and phosphatidyl-inositol phospholipase C (rPI-PLC). Indirect ELISA with the above recombinant antigens was used on samples collected from bovines (n=106), goats (n=138) and pigs (n=92) having either a history of abortion, emaciation and/or apparently healthy animals. Isolation of Listeria was attempted from the blood samples using USDA-FSIS method. On screening of test sera by rLLO-based ELISA, antibodies against anti-listeriolysin O (ALLO) were observed in goats (22...
June 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28400017/screening-of-the-two-component-system-histidine-kinases-of-listeria-monocytogenes-egd-e-lias-is-needed-for-growth-under-heat-acid-alkali-osmotic-ethanol-and-oxidative-stresses
#19
Anna Pöntinen, Miia Lindström, Mikael Skurnik, Hannu Korkeala
To study the role of each two-component system (TCS) histidine kinase (HK) in stress tolerance of Listeria monocytogenes EGD-e, we monitored the growth of individual HK deletion mutant strains under heat (42.5 °C), acid (pH 5.6), alkali (pH 9.4), osmotic (6% NaCl), ethanol (3.5 vol%), and oxidative (5 mM H2O2) stresses. The growth of ΔliaS (Δlmo1021) strain was impaired under each stress, with the most notable decrease under heat and osmotic stresses. The ΔvirS (Δlmo1741) strain showed nearly completely restricted growth at high temperature and impaired growth in ethanol...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28399176/pathological-findings-in-the-red-fox-vulpes-vulpes-stone-marten-martes-foina-and-raccoon-dog-nyctereutes-procyonoides-with-special-emphasis-on-infectious-and-zoonotic-agents-in-northern-germany
#20
Charlotte Lempp, Nicole Jungwirth, Miguel L Grilo, Anja Reckendorf, Arlena Ulrich, Abbo van Neer, Rogier Bodewes, Vanessa M Pfankuche, Christian Bauer, Albert D M E Osterhaus, Wolfgang Baumgärtner, Ursula Siebert
Anthropogenic landscape changes contributed to the reduction of availability of habitats to wild animals. Hence, the presence of wild terrestrial carnivores in urban and peri-urban sites has increased considerably over the years implying an increased risk of interspecies spillover of infectious diseases and the transmission of zoonoses. The present study provides a detailed characterisation of the health status of the red fox (Vulpes vulpes), stone marten (Martes foina) and raccoon dog (Nyctereutes procyonoides) in their natural rural and peri-urban habitats in Schleswig-Holstein, Germany between November 2013 and January 2016 with focus on zoonoses and infectious diseases that are potentially threatening to other wildlife or domestic animal species...
2017: PloS One
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