Lorena Polloni, Tássia Rafaella Costa, Lorena Pinheiro Morais, Bruna Cristina Borges, Samuel Cota Teixeira, Thales Alves de Melo Fernandes, Lucas Ian Veloso Correia, Luciana Machado Bastos, Fernanda Gobbi Amorim, Loïc Quinton, Andreimar Martins Soares, Marcelo José Barbosa Silva, Eloisa Amália Vieira Ferro, Daiana Silva Lopes, Veridiana de Melo Rodrigues Ávila
Cancer cells produce abnormal levels of reactive oxygen species (ROS) that contribute to promote their malignant phenotype. In this framework, we hypothesized that the change in ROS concentration above threshold could impair key events of prostate cancer cells (PC-3) progression. Our results demonstrated that Pollonein-LAAO, a new L-amino acid oxidase obtained from Bothrops moojeni venom, was cytotoxic to PC-3 cells in two-dimensional and in tumor spheroid assays. Pollonein-LAAO was able to increase the intracellular ROS generation that culminates in cell death from apoptosis by both intrinsic and extrinsic pathways due to the up-regulation of TP53, BAX, BAD, TNFRSF10B and CASP8...
June 24, 2023: Cellular Signalling