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citric acid cycle

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https://www.readbyqxmd.com/read/29244243/metabolic-interaction-between-urea-cycle-and-citric-acid-cycle-shunt-a-guided-approach
#1
Rossana Pesi, Francesco Balestri, Piero L Ipata
This article is a guided pedagogical approach, devoted to postgraduate students specializing in biochemistry, aimed at presenting all single reactions and overall equations leading to the metabolic interaction between ureagenesis and citric acid cycle to be incorporated into a two-three lecture series about the interaction of urea cycle with other metabolic pathways. We emphasize that citrate synthetase, aconitase, and isocitrate dehydrogenase, three enzymes of the citric acid cycle are not involved, thus creating a shunt in citric acid cycle...
December 15, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29243276/the-influence-of-shc-proteins-and-high-fat-diet-on-energy-metabolism-of-mice
#2
W A Baldassini, J J Ramsey, K Hagopian, D P D Lanna
The purpose of this study was to determine if Shc proteins influence the metabolic response to acute (7 days) feeding of a high-fat diet (HFD). To this end, whole animal energy expenditure (EE) and substrate oxidation were measured in the Shc knockout (ShcKO) and wild-type (WT) mice fed a control or HFD. The activities of enzymes of glycolysis, the citric acid cycle, electron transport chain (ETC), and β-oxidation were also investigated in liver and skeletal muscle of ShcKO and WT animals. The study showed that ShcKO increases (P < ...
December 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/29235277/-targets-identification-and-mechanism-analysis-for-macrophage-activation-of-low-molecular-weight-saccharides-from-cistanche-deserticola-based-on-molecular-affinity-chromatography
#3
Li-Chao Wang, Dan Liu, Yong Jiang, Peng-Fei Tu, Ke-Wu Zeng
This study aims to investigate the targets and targets-involved mechanism for the macrophage activation of low molecular weight saccharides from Cistanche deserticola (LMSC). The phagocytic activity and NO release of RAW264.7 cells were detected, and results showed that LMSC exerts immune activation effect by significantly increasing the phagocytic activity and NO release. LMSC-conjugated epoxy-activated sepharose beads were prepared as affinity reagent to capture the target proteins. Twenty-four proteins such as Eef2 were identified by LC-MS/MS analysis...
October 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29233539/effect-of-tetracalcium-phosphate-monetite-toothpaste-on-dentin-remineralization-and-tubule-occlusion-in-vitro
#4
L Medvecky, R Stulajterova, M Giretova, J Mincik, M Vojtko, J Balko, J Briancin
OBJECTIVES: To investigate the tubule occlusion and remineralization potential of a novel toothpaste with active tetracalcium phosphate/monetite mixtures under de/remineralization cycling. METHODS: Dentin de/remineralization cycling protocol consisted of demineralization in 1% citric acid at pH 4.6 with following remineralization with toothpastes and soaking in artificial saliva. Effectiveness of toothpastes to promote remineralization was evaluated by measurement of microhardness recovery, analysis of surface roughness, thickness of coating and scanning electron microscopy...
December 9, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29216582/cellular-and-mitochondrial-determination-of-low-molecular-mass-organic-acids-by-lc-ms-ms
#5
Jessica Fiori, Elisa Amadesi, Flaminia Fanelli, Concetta Valentina Tropeano, Michela Rugolo, Roberto Gotti
A selective and sensitive method for the determination of low molecular mass organic acids (LMMOAs) in cell and mitochondrial extracts is presented. The analytical method consists in the separation by reversed phase liquid chromatography and detection with tandem mass spectrometry (LC-MS/MS) of the LMMOAs like malic, succinic, formic and citric acids. These acids are among the cellular intermediates of the tricarboxylic acid cycle (TCA), thus their quantitation can provide essential information about the catabolic and anabolic processes occurring in cells under physiological and pathological conditions...
November 29, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29211784/urinary-metabolomic-analysis-to-identify-preterm-neonates-exposed-to-histological-chorioamnionitis-a-pilot-study
#6
Claudia Fattuoni, Carlo Pietrasanta, Lorenza Pugni, Andrea Ronchi, Francesco Palmas, Luigi Barberini, Angelica Dessì, Roberta Pintus, Vassilios Fanos, Antonio Noto, Fabio Mosca
OBJECTIVE: Chorioamnionitis is a leading cause of preterm birth worldwide, with higher incidence at lower gestational ages. An early and reliable diagnosis of histological chorioamnionitis (HCA) in preterm infants may be helpful in guiding postnatal management, especially the administration of prophylactic antibiotics to prevent early-onset sepsis. The main aim of this study was to investigate metabolomic analysis of urines collected in the first 24 hours of life as diagnostic tool of HCA...
2017: PloS One
https://www.readbyqxmd.com/read/29208371/integrated-semi-targeted-metabolomics-analysis-reveals-distinct-metabolic-dysregulation-in-pleural-effusion-caused-by-tuberculosis-and-malignancy
#7
Nanying Che, Yan Ma, Huabin Ruan, Lina Xu, Xueying Wang, Xinting Yang, Xiaohui Liu
BACKGROUND: Tuberculous pleural effusion (TPE) and malignant pleural effusion (MPE) are the 2 most frequent causes of exudative pleural effusions (PEs). However, the clinical differentiation is challenging. METHODS: Metabolic signatures in pleural effusion from 156 patients were profiled. An integrated semi-targeted metabolomics platform was incorporated for high throughput metabolite identification and quantitation. In this platform, orbitrap based mass spectrometry with data dependent MS/MS acquisition was applied in the analysis...
December 2, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29207610/integration-of-micrornaome-proteomics-and-metabolomics-to-analyze-arsenic-induced-malignant-cell-transformation
#8
Youyou Zhou, Yanfu Wang, Juan Su, Zheng Wu, Chao Wang, Weiming Zhong, Xiaomei Liu, Linhui Cui, Xiaoyu Zhou, Yufang Ma, Yi Xin, Jianglin Zhang, Lisha Wu, Xing Hu, Xiang Chen, Cong Peng, MingYang Gao
Long-term exposure to arsenic has been linked to tumorigenesis in different organs and tissues, such as skin; however, the detailed mechanism remains unclear. In this present study, we integrated "omics" including microRNAome, proteomics and metabolomics to investigate the potential molecular mechanisms. Compared with non-malignant human keratinocytes (HaCaT), twenty-six miRNAs were significantly altered in arsenic-induced transformed cells. Among these miRNAs, the differential expression of six miRNAs was confirmed using Q-RT-PCR, representing potential oxidative stress genes...
October 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/29207315/8-oxoguanine-dna-glycosylase-ogg1-controls-hepatic-gluconeogenesis
#9
Katja Scheffler, Lyudmila Rachek, Panpan You, Alexander D Rowe, Wei Wang, Anna Kuśnierczyk, Lene Kittelsen, Magnar Bjørås, Lars Eide
Mitochondrial DNA (mtDNA) resides in close proximity to metabolic reactions, and is maintained by the 8-oxoguanine DNA glycosylase (Ogg1) and other members of the base excision repair pathway. Here, we tested the hypothesis that changes in liver metabolism as under fasting/feeding conditions would be sensed by liver mtDNA, and that Ogg1 deficient mice might unravel a metabolic phenotype. Wild type (WT) and ogg1-/- mice were either fed ad libitum or subjected to fasting for 24h, and the corresponding effects on liver gene expression, DNA damage, as well as serum values were analyzed...
November 28, 2017: DNA Repair
https://www.readbyqxmd.com/read/29197344/lactation-related-metabolic-mechanism-investigated-based-on-mammary-gland-metabolomics-and-4-biofluids-metabolomics-relationships-in-dairy-cows
#10
Hui-Zeng Sun, Kai Shi, Xue-Hui Wu, Ming-Yuan Xue, Zi-Hai Wei, Jian-Xin Liu, Hong-Yun Liu
BACKGROUND: Lactation is extremely important for dairy cows; however, the understanding of the underlying metabolic mechanisms is very limited. This study was conducted to investigate the inherent metabolic patterns during lactation using the overall biofluid metabolomics and the metabolic differences from non-lactation periods, as determined using partial tissue-metabolomics. We analyzed the metabolomic profiles of four biofluids (rumen fluid, serum, milk and urine) and their relationships in six mid-lactation Holstein cows and compared their mammary gland (MG) metabolomic profiles with those of six non-lactating cows by using gas chromatography-time of flight/mass spectrometry...
December 2, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29187232/long-term-continuous-renal-replacement-therapy-and-anticoagulation-with-citrate-in-critically-ill-patients-with-severe-liver-dysfunction
#11
Matthias Klingele, Theresa Stadler, Danilo Fliser, Timo Speer, Heinrich V Groesdonk, Alexander Raddatz
BACKGROUND: As of 2009, anticoagulation with citrate was standard practice in continuous renal replacement therapy (CRRT) for critically ill patients at the University Medical Centre of Saarland, Germany. Partial hepatic metabolism of citrate means accumulation may occur during CRRT in critically ill patients with impaired liver function. The aim of this study was to evaluate the actual influence of hepatic function on citrate-associated complications during long-term CRRT. METHODS: In a retrospective study conducted between January 2009 and November 2012, all cases of dialysis therapy performed in the interdisciplinary surgical intensive care unit were analysed...
November 29, 2017: Critical Care: the Official Journal of the Critical Care Forum
https://www.readbyqxmd.com/read/29186773/metabolic-perturbations-in-a-bacillus-subtilis-clpp-mutant-during-glucose-starvation
#12
(no author information available yet)
Proteolysis is essential for all living organisms to maintain the protein homeostasis and to adapt to changing environmental conditions. ClpP is the main protease in Bacillus subtilis, and forms complexes with different Clp ATPases. These complexes play crucial roles during heat stress, but also in sporulation or cell morphology. Especially enzymes of cell wall-, amino acid-, and nucleic acid biosynthesis are known substrates of the protease ClpP during glucose starvation. The aim of this study was to analyze the influence of a clpP mutation on the metabolism in different growth phases and to search for putative new ClpP substrates...
November 24, 2017: Metabolites
https://www.readbyqxmd.com/read/29179970/an-interproximal-model-to-determine-the-erosion-protective-effect-of-calcium-silicate-sodium-phosphate-fluoride-formulations
#13
Natalie J Wood, Siân Bodfel Jones, Nick Chapman, Andrew Joiner, Carole J Philpotts, Nicola X West
OBJECTIVES: Previous work has shown the effectiveness of a newly developed interproximal model to differentiate between the amount of remineralization caused by toothpastes used with or without a dual-phase gel treatment system containing calcium silicate, sodium phosphate salts and fluoride to repair acid-softened enamel. The aim of this study was to utilize the same interproximal model to identify how effective calcium silicate phosphate toothpastes are at reducing surface softening in the early stages of erosion...
November 24, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29178759/the-abrasive-effect-of-commercial-whitening-toothpastes-on-eroded-enamel
#14
Victor Mosquim, Beatriz Martines Souza, Gerson Aparecido Foratori Junior, Linda Wang, Ana Carolina Magalhães
PURPOSE: To evaluate the in vitro abrasive effect of commercial whitening toothpastes on eroded bovine enamel samples in respect to erosive tooth wear. METHODS: 72 bovine crowns were embedded, polished and subjected to the baseline profile analysis. The samples were then protected in 2/3 of the enamel surface and were randomly assigned to six groups (n= 12/group): G1: Oral-B 3D White, G2: Close-up Diamond Attraction Power White, G3: Sorriso Xtreme White 4D, G4: Colgate Luminous White, G5: Crest (conventional toothpaste), G6:erosion only (control)...
June 2017: American Journal of Dentistry
https://www.readbyqxmd.com/read/29177882/effect-of-the-natural-cytokine-complex-on-the-structure-and-metabolism-of-contractile-myocardium-normally-and-under-increased-hemodynamic-load
#15
M S Tverskaya, L V Gankovskaya, V V Sukhoparova, A O Virganskii
Effect of natural complex of cytokines with activity of IL-1, IL-2, IL-6, TNF, MIF, GTFβ on the structure and metabolism of contractile ventricular cardiomyocytes was assessed in the control and under conditions of acute experimental aortic stenosis. Systemic administration of the complex in the control had no significant effect on myocardial morphology with low number of damaged cardiomyocytes and low degree of structural damage. Administration of the cytokine complex against the background of aortic stenosis did not exert any additional alterative effect on cardiomyocytes, structural damage of contractual nature was moderate...
November 27, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29171938/complementary-strategies-towards-the-aqueous-processing-of-high-voltage-lini0-5mn1-5o4-lithium-ion-cathodes-crosslinking-cmc-and-phosphoric-acid-treatment
#16
Matthias Kuenzel, Dominic Bresser, Thomas Diemant, Diogo Viera Carvalho, Guk-Tae Kim, Juergen Behm, Stefano Passerini
Increasing the environmental benignity of lithium-ion batteries is one of the greatest challenges for their large-scale deployment. Toward this end, we present herein a strategy to enable the aqueous processing of high-voltage LiNi0.5Mn1.5O4 (LNMO) cathodes, which are considered highly, if not the most, promising for the realization of cobalt-free next generation lithium-ion cathodes. Combining the addition of phosphoric acid with the crosslinking of sodium carboxymethyl cellulose by means of citric acid, aqueously processed electrodes with excellent performance are produced...
November 24, 2017: ChemSusChem
https://www.readbyqxmd.com/read/29161329/comparative-metabolomics-profiling-of-engineered-saccharomyces-cerevisiae-lead-to-a-strategy-that-improving-%C3%AE-carotene-production-by-acetate-supplementation
#17
Xiao Bu, Liang Sun, Fei Shang, Guoliang Yan
A comparative metabolomic analysis was conducted on recombinant Saccharomyces cerevisiae strain producing β-carotene and the parent strain cultivated with glucose as carbon source using gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography-mass spectrometry (HPLC-MS) and ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) based approach. The results showed that most of the central intermediates associated with amino acids, carbohydrates, glycolysis and TCA cycle intermediates (acetic acid, glycerol, citric acid, pyruvic acid and succinic acid), fatty acids, ergosterol and energy metabolites were produced in a lower amount in recombinant strain, as compared to the parent strain...
2017: PloS One
https://www.readbyqxmd.com/read/29157838/impaired-citric-acid-cycle-in-nondiabetic-chronic-kidney-disease
#18
Zhanjun Jia
No abstract text is available yet for this article.
November 14, 2017: EBioMedicine
https://www.readbyqxmd.com/read/29156417/the-effect-of-magnesium-hydroxide-containing-dentifrice-using-an-extrinsic-and-intrinsic-erosion-cycling-model
#19
Vanara Florêncio Passos, Lidiany Karla Azevedo Rodrigues, Sérgio Lima Santiago
OBJECTIVE: To evaluate, in vitro, the effect of Mg(OH)2 dentifrice, and the influence of the number of experimental days, on the extrinsic (citric acid -CA) and intrinsic (hydrochloric acid -HCl) enamel erosion models. DESIGN: Human enamel slabs were selected according to surface hardness and randomly assigned to 3 groups (n=9) as follows: non-fluoridated (negative control), NaF (1450ppm F- positive control) and Mg(OH)2 (2%) dentifrices. The slabs were daily submitted to a 2-h period of pellicle formation and, over a period of 5days, submitted to cycles (3×/day) of erosive challenge (CA 0...
November 14, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29152696/degradation-of-the-earthy-and-musty-odorant-2-4-6-tricholoroanisole-by-persulfate-activated-with-iron-of-different-valences
#20
Kejia Zhang, Xinyan Zhou, Tuqiao Zhang, Lang Yu, Zheming Qian, Wenchao Liao, Cong Li
2,4,6-Trichloroanisole (TCA) is an odorous compound that is often detected in tap water and is difficult to be removed via water treatment. In this study, the transformation efficiency of TCA in the presence of persulfate (PS) activated by iron (Fe(2+), Fe(0), and Fe(3+)) was investigated for the first time. The effects of the activator dosage, oxidant dosage, pH, dosing method, chelating agents, humic acid, and temperature were evaluated. The degradation rate of TCA increased with increasing PS dosages (0...
November 19, 2017: Environmental Science and Pollution Research International
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