Read by QxMD icon Read


Mario Agurto, Rudolf O Schlechter, Grace Armijo, Esteban Solano, Carolina Serrano, Rodrigo A Contreras, Gustavo E Zúñiga, Patricio Arce-Johnson
Fungal pathogens are the cause of the most common diseases in grapevine and among them powdery mildew represents a major focus for disease management. Different strategies for introgression of resistance in grapevine are currently undertaken in breeding programs. For example, introgression of several resistance genes (R) from different sources for making it more durable and also strengthening the plant defense response. Taking this into account, we cross-pollinated P09-105/34, a grapevine plant carrying both RUN1 and REN1 pyramided loci of resistance to Erysiphe necator inherited from a pseudo-backcrossing scheme with Muscadinia rotundifolia and Vitis vinifera 'Dzhandzhal Kara,' respectively, with the susceptible commercial table grape cv...
2017: Frontiers in Plant Science
Dániel Pap, Summaira Riaz, Ian B Dry, Angelica Jermakow, Alan C Tenscher, Dario Cantu, Róbert Oláh, M Andrew Walker
BACKGROUND: Grapevine powdery mildew Erysiphe necator is a major fungal disease in all grape growing countries worldwide. Breeding for resistance to this disease is crucial to avoid extensive fungicide applications that are costly, labor intensive and may have detrimental effects on the environment. In the past decade, Chinese Vitis species have attracted attention from grape breeders because of their strong resistance to powdery mildew and their lack of negative fruit quality attributes that are often present in resistant North American species...
July 29, 2016: BMC Plant Biology
Paola Barba, Lance Cadle-Davidson, Erin Galarneau, Bruce Reisch
Vitis rupestris B38 is a North American grapevine resistant to the major pathogen of cultivated grapevines, Erysiphe necator. Sources of powdery mildew resistance, like V. rotundifolia, are widely used in grape breeding but are already threatened, even before commercialization, by isolates that can reproduce on Run1 and other rotundifolia-derived breeding lines. Thus, complementary sources of resistance are needed to improve resistance durability. The segregation of foliar powdery mildew severity in an F1 family, derived from a cross of V...
August 2015: Phytopathology
Angela Feechan, Marianna Kocsis, Summaira Riaz, Wei Zhang, David M Gadoury, M Andrew Walker, Ian B Dry, Bruce Reisch, Lance Cadle-Davidson
The Toll/interleukin-1 receptor nucleotide-binding site leucine-rich repeat gene, "resistance to Uncinula necator 1" (RUN1), from Vitis rotundifolia was recently identified and confirmed to confer resistance to the grapevine powdery mildew fungus Erysiphe necator (syn. U. necator) in transgenic V. vinifera cultivars. However, sporulating powdery mildew colonies and cleistothecia of the heterothallic pathogen have been found on introgression lines containing the RUN1 locus growing in New York (NY). Two E. necator isolates collected from RUN1 vines were designated NY1-131 and NY1-137 and were used in this study to inform a strategy for durable RUN1 deployment...
August 2015: Phytopathology
Tahereh Jafarzadeh Ghehi, Soheil Mortezaeifar, Mitra Gholami, Roshanak Rezaei Kalantary, Amir Hossein Mahvi
BACKGROUND: Simultaneous nitrogen, phosphorous and COD removal in a pilot-scale enhanced Sequencing Batch Reactor (eSBR) was investigated. METHODS: The reactor consisted of a pre-anoxic zone and internal recycle and was fed with synthetic wastewater. The study was performed by operating the reactor in 6-hour cycles in three different operational modes during a time frame of 279 days. RESULTS: Under the best operational conditions, the average removal rate of COD, TN, and TP were obtained as 93...
2014: Journal of Environmental Health Science & Engineering
Nedal Massalha, Asher Brenner, Chaim Sheindorf, Isam Sabbah
The main goal of this study was to test the effect of various drying methods of granular anaerobic biomass on biomass survival, potential and rate of methane re-production, and structure. This may facilitate the development of drying methods to preserve excess anaerobic biomass in dry form for re-inoculation of existing digesters after process failure or wash out or for the start-up of new digesters. To that end, anaerobic granular biomass was collected from an up-flow anaerobic sludge blanket (UASB) reactor...
October 15, 2014: Water Research
Olumide David Olukanni, Akinniyi A Osuntoki, Ayodeji Olushola Awotula, Dayanand C Kalyani, George Olabode Gbenle, Sanjay P Govindwar
A dye-decolorizing bacterium was isolated from a soil sample and identified as Bacillus thuringiensis using 16S rRNA sequencing. The bacterium was able to decolorize three different textile dyes, namely, Reactive blue 13, Reactive red 58, and Reactive yellow 42, and a real dyehouse effluent up to 80-95% within 6 h. Some non-textile industrially important dyes were also decolorized to different extents. Fourier transform infrared spectroscopy and gas chromatography-mass spectrometer analysis of the ethyl acetate extract of Congo red dye and its metabolites showed that the bacterium could degrade it by the asymmetric cleavage of the azo bonds to yield sodium (4- amino-3-diazenylnaphthalene-1-sulfonate) and phenylbenzene...
June 28, 2013: Journal of Microbiology and Biotechnology
Qifan Sun, Chuanhui Han, Lan Liu, Yizhi Wang, Hongyu Deng, Lin Bai, Tao Jiang
NESCA, a newly discovered signaling adapter protein in the NGF-pathway, contains a RUN domain at its N-terminus. Here we report the crystal structure of the NESCA RUN domain determined at 2.0-Å resolution. The overall fold of the NESCA RUN domain comprises nine helices, resembling the RUN domain of RPIPx and the RUN1 domain of Rab6IP1. However, compared to the other RUN domains, the RUN domain of NESCA has significantly different surface electrostatic distributions at the putative GTPase-interacting interface...
August 2012: Protein & Cell
Humberto Fernandes, Edward Franklin, Florence Jollivet, Katharina Bliedtner, Amir R Khan
Eukaryotic cells have developed a diverse repertoire of Rab GTPases to regulate vesicle trafficking pathways. Together with their effector proteins, Rabs mediate various aspects of vesicle formation, tethering, docking and fusion, but details of the biological roles elicited by effectors are largely unknown. Human Rab6 is involved in the trafficking of vesicles at the level of Golgi via interactions with numerous effector proteins. We have previously determined the crystal structure of Rab6 in complex with DENND5, alternatively called Rab6IP1, which comprises two RUN domains (RUN1 and RUN2) separated by a PLAT domain...
2012: PloS One
Humberto Fernandes, Edward Franklin, Amir R Khan
Effectors of the Rab small GTPases are large multi-domain proteins which have proved difficult to express in soluble form in Escherichia coli. Generally, effectors are recruited to a distinct subcellular compartment by active (GTP-bound) Rabs, which are linked to membranes by one or two prenylated Cys residues at their C-termini. Following recruitment via their Rab-binding domain (RBD), effectors carry out various aspects of vesicle formation, transport, tethering and fusion through their other domains. Previously, successful purification of the RUN-PLAT tandem domains (residues 683-1061) of the 1263-residue Rab6-interacting protein 1 (R6IP1) required co-expression with Rab6, as attempts to solubly express the effector alone were unsuccessful...
May 1, 2011: Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Jinlei Nie, Keith P George, Tom K Tong, Ye Tian, Qingde Shi
PURPOSE: This study examined the effect of two bouts of prolonged exercise performed on the same day on cardiac biomarkers and left ventricular (LV) function in trained adolescent runners. METHODS: Twelve male adolescent runners (age = 14.5 ± 1.5 yr) performed two 45-min constant-load treadmill runs (RUN1 and RUN2), separated by 255 min of recovery, with intensity set at the running speeds that corresponded to ventilatory threshold. Serum cardiac troponin T (cTnT) and N-terminal pro-brain natriuretic peptide (NT-pro-BNP) were assessed before (PRE1, PRE2), immediately after (POST1, POST2), and 255 min after each run (POST1+4, POST2+4) where PRE2 and POST1+4 were the same sample...
November 2011: Medicine and Science in Sports and Exercise
Feifei Wang, Yuanhong Ding, Lei Ge, Hongqiang Ren, Lili Ding
The effect of high-strength ammonia nitrogen acclimation on sludge activity in sequencing batch reactor (SBR) was investigated. Two batch experiments, RUN1 and RUN2, were conducted with the influent ammonia nitrogen concentrations 60 and 500 mg/L, respectively. The sludges inoculated from RUN1 and RUN2 were used to treat a series of influent with ammonia nitrogen concentrations of 59, 232, 368, 604 and 1152 mg/L. It is found that the activated sludge acclimated to higher ammonia nitrogen concentrations revealed higher COD and NH4(+)-N removal efficiencies, and slower DHA decrease...
2010: Journal of Environmental Sciences (China)
S Riaz, A C Tenscher, D W Ramming, M A Walker
A limited genetic mapping strategy based on simple sequence repeat (SSR) marker data was used with five grape populations segregating for powdery mildew (Erysiphe necator) resistance in an effort to develop genetic markers from multiple sources and enable the pyramiding of resistance loci. Three populations derived their resistance from Muscadinia rotundifolia 'Magnolia'. The first population (06708) had 97 progeny and was screened with 137 SSR markers from seven chromosomes (4, 7, 9, 12, 13, 15, and 18) that have been reported to be associated with powdery or downy mildew resistance...
April 2011: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
I Mishima, J Nakajima
The control of membrane fouling is an essential issue in membrane bioreactor (MBR) process. It has been recognized that the most important factors that affect membrane fouling are presence of soluble microbial products (SMP) and extracellular polymeric substances (EPS) in a reactor. The objective of this study was to examine the effect of the coagulant addition on the membrane fouling in the MBR process. Accordingly, laboratory scale batch experiments and MBR experiments were conducted using coagulant. In batch experiments, effective SMP removal and control of EPS release were observed by coagulant addition...
2009: Water Science and Technology: a Journal of the International Association on Water Pollution Research
S W Chung, H S Lee
In monsoon climate area, turbidity flows typically induced by flood runoffs cause numerous environmental impacts such as impairment of fish habitat and river attraction, and degradation of water supply efficiency. This study was aimed to characterize the physical dynamics of turbidity plume induced into a stratified reservoir using field monitoring and numerical simulations, and to assess the effect of different withdrawal scenarios on the control of downstream water quality. Three different turbidity models (RUN1, RUN2, RUN3) were developed based on a two-dimensional laterally averaged hydrodynamic and transport model, and validated against field data...
2009: Water Science and Technology: a Journal of the International Association on Water Pollution Research
Y J Wu, L M Whang, S J Huang, Y F Yang, C N Lei, S S Cheng
In Taiwan, a substantial amount of thin-film transistor liquid crystal display (TFT-LCD) wastewater is produced daily due to an increasing production of the opto-electronic industry in recent years. The main components of TFT-LCD wastewater include dimethyl sulphoxide (DMSO), monoethanolamine (MEA), and tetra-methyl ammonium hydroxide (TMAH), which are recognized as non-or slow-biodegradable organic compounds and limited information is available regarding their biological treatablility. This study was conducted to evaluate the long-term performance of two bioreactors, anaerobic-aerobic (A/O) sequencing batch reactor (SBR) and aerobic membrane bioreactor (MBR), treating synthetic TFT-LCD wastewater containing DMSO, MEA, and TMAH with different loadings...
2008: Water Science and Technology: a Journal of the International Association on Water Pollution Research
Hai-jun Zhang, Yu-wen Ni, Xue-ping Zhang, Qing Zhang, Liang Zhao, Ning Zhang, Ji-ping Chen
PCDD/F distributions in a two-stage reciprocating grate municipal solid waste (MSW) incinerator under two kinds of operating conditions were investigated by sampling the ashes, flue gases and bottom ashes at different positions, and the mass balance was calculated. At the boiler outlet, the concentration of PCDD/F in the flue gas is 2.602 ng x m(-3) and 1.236 ng x m(-3) under operating condition 1 (RUNI) and operating condition 2 (RUN2), respectively. A significant formation of PCDD/F was found in the sections of semi-dry scrubber (SDS) and cyclone precipitator (CP), in which the temperature of flue gas was in the range of 260-180 degrees C...
April 2008: Huan Jing Ke Xue= Huanjing Kexue
Lucille L Smith, Andrew J McKune, Stuart J Semple, Emmanuel Sibanda, Helen Steel, Ronald Anderson
The purpose of this study was to examine changes in serum cytokines after repeated bouts of aerobically biased eccentric exercise. Six untrained males ran down a -13.5% treadmill grade for 60 min on two occasions (RUN1 and RUN2) at a speed equal to 75% of their VO2 peak on a level grade; runs were spaced 14 d apart. Serum was collected before, after, and every hour for 12 h, and every 24 h for 6 d. Cytokines were assessed using 17 multiplex bead technology (Bio-Rad). Creatine kinase (CK) and delayed-onset muscle soreness (DOMS) were assessed before and 24-120 h after...
April 2007: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
C L Barker, T Donald, J Pauquet, M B Ratnaparkhe, A Bouquet, A-F Adam-Blondon, M R Thomas, I Dry
Resistance to grapevine powdery mildew is controlled by Run1, a single dominant gene present in the wild grapevine species, Muscadinia rotundifolia, but absent from the cultivated species, Vitis vinifera. Run1 has been introgressed into V. vinifera using a pseudo-backcross strategy, and genetic markers have previously been identified that are linked to the resistance locus. Here we describe the construction of comprehensive genetic and physical maps spanning the resistance locus that will enable future positional cloning of the resistance gene...
July 2005: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
T. M. Donald, F. Pellerone, A.-F. Adam-Blondon, A. Bouquet, M. R. Thomas, I. B. Dry
Oligonucleotide primers, designed to conserved regions of nucleotide binding site (NBS) motifs within previously cloned pathogen resistance genes, were used to amplify resistance gene analogs (RGAs) from grapevine. Twenty eight unique grapevine RGA sequences were identified and subdivided into 22 groups on the basis of nucleic acid sequence-identity of approximately 70% or greater. Representatives from each group were used in a bulked segregant analysis strategy to screen for restriction fragment length polymorphisms linked to the powdery mildew resistance locus, Run1, introgressed into Vitis vinifera L...
March 2002: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"