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Qtracker stem cell

Toni Uhlendorf, Ruslan Nuryyev, Alex Kopyov, Jessica Ochoa, Shahab Younesi, Randy Cohen, Oleg Kopyov
<p>An emerging avenue for recalcitrant neurodegenerative disease treatment is neural progenitor cell (NPC) transplantation. In this study, we investigated the effectiveness of two different delivery routes of human-derived NPC inoculation: injection into the common carotid artery or unilateral stereotactic implantation into the degenerating cerebellum and hippocampus of spastic Han-Wistar (sHW) rats, a model of ataxia.</p><p>At 30 days of age, sHW mutants were implanted with osmotic pumps pre-loaded with cyclosporine...
7, 2016: Cell Transplantation
Francesca Nicholls, Jessie Liu, Michel Modo
The interpretation of cell transplantation experiments is often dependent on the presence of an exogenous label for theidentification of implanted cells. The exogenous labels Hoechst 33342, 5-Bromo-2’-deoxyuridine (BrdU), PKH26, and Qtracker were compared for their labeling efficiency, cellular effects and reliability to identify a human neural stem cell (hNSC) line implanted intracerebrally into the rat brain. Hoechst 33342 (2 mg/mL) exhibited a delayed cytotoxicity that killed all cells within 7 days...
3, 2016: Cell Transplantation
Evgeny Kushnerev, Susan G Shawcross, Shankari Sothirachagan, Fiona Carley, Arun Brahma, Julian M Yates, M Chantal Hillarby
Purpose: The corneal epithelium is sloughed off surface of the eye by the action of blinking and is continually replaced by division and maturation of the limbal stem cells (LSCs). In the case of injury or disease, LSCs can be lost or damaged to a point at which the corneal epithelial layer is no longer maintained. leading to LSC deficiencies (LSCDs). When this occurs, the opaque conjunctiva overgrows the anterior surface of the eye, leading to vision impairment or loss. Dental pulp stem cells (DPSCs) are promising candidates as autologous LSC substitutes...
October 1, 2016: Investigative Ophthalmology & Visual Science
Meng Gao, Junjian Chen, Gengwei Lin, Shiwu Li, Lin Wang, Anjun Qin, Zujin Zhao, Li Ren, Yingjun Wang, Ben Zhong Tang
Bone marrow-derived mesenchymal stem cells (BMSCs) have shown great potential for bone repair due to their strong proliferation ability and osteogenic capacity. To evaluate and improve the stem cell-based therapy, long-term tracking of stem cell differentiation into bone-forming osteoblasts is required. However, conventional fluorescent trackers such as fluorescent proteins, quantum dots, and fluorophores with aggregation-caused quenching (ACQ) characteristics have intrinsic limitations of possible interference with stem cell differentiation, heavy metal cytotoxicity, and self-quenching at a high labeling intensity...
July 20, 2016: ACS Applied Materials & Interfaces
Dan Ding, Duo Mao, Kai Li, Xiaomin Wang, Wei Qin, Rongrong Liu, David Shunzhong Chiam, Nikodem Tomczak, Zhimou Yang, Ben Zhong Tang, Deling Kong, Bin Liu
Monitoring and understanding long-term fate and regenerative therapy of administrated stem cells in vivo is of great importance. Herein we report organic nanodots with aggregation-induced emission characteristics (AIE dots) for long-term tracking of adipose-derived stem cells (ADSCs) and their regenerative capacity in living mice. The AIE dots possess high fluorescence (with a high quantum yield of 25±1%), excellent biological and photophysical stabilities, low in vivo toxicity, and superb retention in living ADSCs with negligible interference on their pluripotency and secretome...
December 23, 2014: ACS Nano
S Danner, H Benzin, T Vollbrandt, J Oder, A Richter, C Kruse
With the increasing relevance of cell-based therapies, there is a demand for cell-labeling techniques for in vitro and in vivo studies. For the reasonable tracking of transplanted stem cells in animal models, the usage of quantum dots (QDs) for sensitive cellular imaging has major advances. QDs could be delivered to the cytoplasm of the cells providing intense and stable fluorescence. Although QDs are emerging as favourable nanoparticles for bioimaging, substantial investigations are still required to consider their application for adult stem cells...
2013: International Journal of Cell Biology
Uwe M Fischer, Matthew T Harting, Fernando Jimenez, Werner O Monzon-Posadas, Hasen Xue, Sean I Savitz, Glen A Laine, Charles S Cox
Intravenous (IV) stem cell delivery for regenerative tissue therapy has been increasingly used in both experimental and clinical trials. However, recent data suggest that the majority of administered stem cells are initially trapped in the lungs. We sought to investigate variables that may affect this pulmonary first-pass effect. In anesthetized Sprague-Dawley rats, silicone tubing catheters were placed in the left internal jugular vein and common carotid artery. We investigated four different cell types: mesenchymal stromal cells (MSC), multipotent adult progenitor cells (MAPCs), bone marrow-derived mononuclear cells (BMMC), and neural stem cells (NSC)...
June 2009: Stem Cells and Development
Shuan Lin, Xiaoyan Xie, Manishkumar R Patel, Yao-Hung Yang, Zongjin Li, Feng Cao, Oliver Gheysens, Yan Zhang, Sanjiv S Gambhir, Jiang Hong Rao, Joseph C Wu
BACKGROUND: Semiconductor quantum dots (QDs) hold increasing potential for cellular imaging both in vitro and in vivo. In this report, we aimed to evaluate in vivo multiplex imaging of mouse embryonic stem (ES) cells labeled with Qtracker delivered quantum dots (QDs). RESULTS: Murine embryonic stem (ES) cells were labeled with six different QDs using Qtracker. ES cell viability, proliferation, and differentiation were not adversely affected by QDs compared with non-labeled control cells (P = NS)...
October 9, 2007: BMC Biotechnology
Satoshi Murasawa, Atsuhiko Kawamoto, Miki Horii, Shuko Nakamori, Takayuki Asahara
OBJECTIVE: Previous studies from our laboratory have shown therapeutic potential of ex vivo expanded endothelial progenitor cells (EPCs) for myocardial ischemia. Our purpose was to investigate the mechanisms regulating EPC contribution to myocardial regeneration. METHODS AND RESULTS: To evaluate niche-dependent expression profiles of EPCs in vitro, we performed coculture using cultured EPCs derived from human peripheral blood and rat cardiac myoblast cell line (H9C2)...
July 2005: Arteriosclerosis, Thrombosis, and Vascular Biology
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