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Tracker stem cell

Xiaolei Cai, Chong-Jing Zhang, Frances Ting Wei Lim, Su Jing Chan, Aishwarya Bandla, Chan Kim Chuan, Fang Hu, Shidang Xu, Nitish V Thakor, Lun-De Liao, Bin Liu
Stem-cell based therapy is an emerging therapeutic approach for ischemic stroke treatment. Bone marrow stromal cells (BMSCs) are in common use as a cell source for stem cell therapy and show promising therapeutic outcomes for stroke treatment. One challenge is to develop a reliable tracking strategy to monitor the fate of BMSCs and assess their therapeutic effects in order to improve the success rate of such treatment. Herein, TPEEP, a fluorogen with aggregation-induced emission characteristics and near-infrared emission are designed and synthesized and further fabricated into organic nanoparticles (NPs)...
September 4, 2016: Small
Meng Gao, Junjian Chen, Gengwei Lin, Shiwu Li, Lin Wang, Anjun Qin, Zujin Zhao, Li Ren, Yingjun Wang, Ben Zhong Tang
Bone marrow-derived mesenchymal stem cells (BMSCs) have shown great potential for bone repair due to their strong proliferation ability and osteogenic capacity. To evaluate and improve the stem cell-based therapy, long-term tracking of stem cell differentiation into bone-forming osteoblasts is required. However, conventional fluorescent trackers such as fluorescent proteins, quantum dots, and fluorophores with aggregation-caused quenching (ACQ) characteristics have intrinsic limitations of possible interference with stem cell differentiation, heavy metal cytotoxicity, and self-quenching at a high labeling intensity...
July 20, 2016: ACS Applied Materials & Interfaces
Hsiangkuo Yuan, Jose A Gomez, Jennifer S Chien, Lunan Zhang, Christy M Wilson, Shuqin Li, Andrew M Fales, Yang Liu, Gerald A Grant, Maria Mirotsou, Victor J Dzau, Tuan Vo-Dinh
High-resolution tracking of stem cells remains a challenging task. An ultra-bright contrast agent with extended intracellular retention is suitable for in vivo high-resolution tracking of stem cells following the implantation. Here, a plasmonic-active nanoplatform was developed for tracking mesenchymal stromal cells (MSCs) in mice. The nanoplatform consisted of TAT peptide-functionalized gold nanostars (TAT-GNS) that emit ultra-bright two-photon photoluminescence capable of tracking MSCs under high-resolution optical imaging...
April 2016: Journal of Biophotonics
Christopher J Hillary, Sabiniano Roman, Anthony J Bullock, Nicola H Green, Christopher R Chapple, Sheila MacNeil
BACKGROUND: Polypropylene mesh used as a mid-urethral sling is associated with severe clinical complications in a significant minority of patients. Current in vitro mechanical testing shows that polypropylene responds inadequately to mechanical distension and is also poor at supporting cell proliferation. AIMS AND OBJECTIVES: Our objective therefore is to produce materials with more appropriate mechanical properties for use as a sling material but which can also support cell integration...
2016: PloS One
Karin E Sisti, María C de Andrés, David Johnston, Edson Almeida-Filho, Antonio C Guastaldi, Richard O C Oreffo
PURPOSE: To evaluate the osteo-regenerative potential of Titanium (Ti) modified by Light Amplification by Stimulated Emission of Radiation (LASER) beam (Yb-YAG) upon culture with human Skeletal Stem Cells (hSSCs(1)). METHODS: Human skeletal cell populations were isolated from the bone marrow of haematologically normal patients undergoing primary total hip replacement following appropriate consent. STRO-1(+) hSSC(1) function was examined for 10 days across four groups using Ti discs: i) machined Ti surface group in basal media (Mb(2)), ii) machined Ti surface group in osteogenic media (Mo(3)), iii) LASER-modified Ti group in basal media (Lb(4)) and, iv) LASER-modified Ti group in osteogenic media (Lo(5))...
May 6, 2016: Biochemical and Biophysical Research Communications
Chunmei Shi, Lingling Zhao, Junjie Wang, Chiping Zhang, Xiaohong Su, Peijun Ma
Tracking micro-objects in the noisy microscopy image sequences is important for the analysis of dynamic processes in biological objects. In this paper, an automated tracking framework is proposed to extract the trajectories of micro-objects. This framework uses a probability hypothesis density particle filtering (PF-PHD) tracker to implement a recursive state estimation and trajectories association. In order to increase the efficiency of this approach, an elliptical target model is presented to describe the micro-objects using shape parameters instead of point-like targets which may cause inaccurate tracking...
April 2016: Journal of Mathematical Biology
C C Ude, M H Ng, C H Chen, O Htwe, N S Amaramalar, S Hassan, I Djordjevic, R A Rani, J Ahmad, N M Yahya, A B Saim, R B Hj Idrus
OBJECTIVES: Our previous studies on osteoarthritis (OA) revealed positive outcome after chondrogenically induced cells treatment. Presently, the functional improvements of these treated OA knee joints were quantified followed by evaluation of the mechanical properties of the engineered cartilages. METHODS: Baseline electromyogram (EMGs) were conducted at week 0 (pre-OA), on the locomotory muscles of nine un-castrated male sheep (Siamese long tail cross) divided into controls, adipose-derived stem cells (ADSCs) and bone marrow stem cells (BMSCs), before OA inductions...
August 2015: Osteoarthritis and Cartilage
Dan Ding, Duo Mao, Kai Li, Xiaomin Wang, Wei Qin, Rongrong Liu, David Shunzhong Chiam, Nikodem Tomczak, Zhimou Yang, Ben Zhong Tang, Deling Kong, Bin Liu
Monitoring and understanding long-term fate and regenerative therapy of administrated stem cells in vivo is of great importance. Herein we report organic nanodots with aggregation-induced emission characteristics (AIE dots) for long-term tracking of adipose-derived stem cells (ADSCs) and their regenerative capacity in living mice. The AIE dots possess high fluorescence (with a high quantum yield of 25±1%), excellent biological and photophysical stabilities, low in vivo toxicity, and superb retention in living ADSCs with negligible interference on their pluripotency and secretome...
December 23, 2014: ACS Nano
Caroline Santos Capitelli, Carolina Salomão Lopes, Angélica Cristina Alves, Janaína Barbiero, Lucas Felipe Oliveira, Valdo José Dias da Silva, Maria Aparecida Barbato Frazão Vital
The 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) animal model is a useful tool to study Parkinson's disease (PD) and was used in the present study to investigate the potential beneficial as well as deleterious effects of systemic bone-marrow mononuclear cell (BMMC) or mesenchymal stem cell (BM-MSC) transplantation. MPTP administration resulted in a breakdown of the blood-brain barrier and motor impairment in the open field test 24 h after surgery. Three and 7 days after receiving the lesion, the injured animals showed remaining motor impairment compared to the sham groups along with a significant loss of tyrosine hydroxylase-immunoreactive (TH-ir) cells in the substantia nigra pars compacta (SNpc)...
2014: International Journal of Medical Sciences
Oksana Kehoe, Alison Cartwright, Ayman Askari, Alicia J El Haj, Jim Middleton
BACKGROUND: Rheumatoid arthritis (RA) is a debilitating and painful disease leading to increased morbidity and mortality and novel therapeutic approaches are needed. The purpose of this study was to elucidate if mesenchymal stem cells (MSCs) injected in the joints of mice with arthritis are therapeutic, reducing joint swelling and cartilage destruction. METHODS: Murine mesenchymal stem cells (mMSCs) were isolated from bone marrow of C57Bl/6 mice and expanded in culture...
2014: Journal of Translational Medicine
R Tao, T-J Sun, Y-Q Han, G Xu, J Liu, Y-F Han
BACKGROUND AND OBJECTIVES: Human umbilical cord-derived mesenchymal stem cells (hUCMSCs) are a novel source of seed cells for cell therapy and tissue engineering. However, in vitro labeling methods for hUCMSCs need to be optimized for better detection of transplanted cells. AIM OF THE STUDY: To identify the most stable and efficient method for labeling hUCMSCs in vitro. MATERIALS AND METHODS: hUCMSCs were isolated using a modified enzymatic digestion procedure and cultured...
2014: European Review for Medical and Pharmacological Sciences
Kerstin Buttler, Muhammad Badar, Virginia Seiffart, Sandra Laggies, Gerhard Gross, Jörg Wilting, Herbert A Weich
Cellular pro-angiogenic therapies may be applicable for the treatment of peripheral vascular diseases. Interactions between mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) may provide such a treatment option. With the exception of some studies in man, experiments have only been performed in immunodeficient mice and rats. We studied an immunocompetent syngeneic mouse model. We isolated MSCs from bone marrow and EPCs from the lung of adult C57/Bl.6 mice and co-injected them in Matrigel subcutaneously in adult C57/Bl...
April 2014: Cellular and Molecular Life Sciences: CMLS
Kai Li, Wei Qin, Dan Ding, Nikodem Tomczak, Junlong Geng, Rongrong Liu, Jianzhao Liu, Xinhai Zhang, Hongwei Liu, Bin Liu, Ben Zhong Tang
Long-term noninvasive cell tracing by fluorescent probes is of great importance to life science and biomedical engineering. For example, understanding genesis, development, invasion and metastasis of cancerous cells and monitoring tissue regeneration after stem cell transplantation require continual tracing of the biological processes by cytocompatible fluorescent probes over a long period of time. In this work, we successfully developed organic far-red/near-infrared dots with aggregation-induced emission (AIE dots) and demonstrated their utilities as long-term cell trackers...
2013: Scientific Reports
Samantha C W Chan, Alexander Bürki, Harald M Bonél, Lorin M Benneker, Benjamin Gantenbein-Ritter
BACKGROUND CONTEXT: Proteolytic enzyme digestion of the intervertebral disc (IVD) offers a method to simulate a condition of disc degeneration for the study of cell-scaffold constructs in the degenerated disc. PURPOSE: To characterize an in vitro disc degeneration model (DDM) of different severities of glycosaminoglycans (GAG) and water loss by using papain, and to determine the initial response of the human mesenchymal stem cells (MSCs) introduced into this DDM...
March 2013: Spine Journal: Official Journal of the North American Spine Society
Nezamoddin N Kachouie, Paul Fieguth, John Ramunas, Eric Jervis
The study of cell behavior is of crucial importance in drug and disease research. The fields of bioinformatics and biotechnology rely on the collection, processing, and analysis of huge numbers of biocellular images, including cell features such as cell size, shape, and motility. However manual methods of inferring these values are so onerous that automated methods of cell tracking and segmentation are in high demand. In this paper, a novel model-based cell tracker is designed to locate and track individual cells...
2006: International Journal of Biomedical Imaging
Krishna C Vallabhaneni, Hermann Haller, Inna Dumler
Multipotent mesenchymal stem cells (MSCs) are promising candidates for regenerative cell-based therapy. The mechanisms underlying MSC differentiation and other functions relevant to therapeutic avenues remain however a matter of debate. Recent reports imply a critical role for intercellular contacts in MSC differentiation. We studied MSC differentiation to vascular smooth muscle cells (VSMCs) in a coculture model using human primary MSCs and VSMCs. We observed that under these conditions, MSCs did not undergo the expected differentiation process...
November 20, 2012: Stem Cells and Development
Chakradhar Velagapudi, Rune-Par Nilsson, Myung Ja Lee, Hannah S Burns, Jill M Ricono, Mazen Arar, Veronique L Barnes, Hanna E Abboud, Jeffrey L Barnes
Kidney development is regulated by a coordinated reciprocal induction of metanephric mesenchymal (MM) and ureteric bud (UB) cells. Here, established MM and UB progenitor cell lines were recombined in three-dimensional Matrigel implants in SCID mice. Differentiation potential was examined for changes in phenotype, organization, and the presence of specialized proteins using immunofluorescence and bright-field and electron microscopy. Both cell types, when grown alone, did not develop into specialized structures...
February 2012: American Journal of Pathology
Chia-Yi Fang, V Vaijayanthimala, Chi-An Cheng, Shih-Hua Yeh, Ching-Fang Chang, Chung-Leung Li, Huan-Cheng Chang
Fluorescent nanodiamond (FND) has excellent biocompatibility and photostability, making it well suited for long-term labeling and tracking of cancer and stem cells. To prove the concept, the exocytosis of FND particles (size ≈100 nm) from three cell lines--HeLa cervical cancer cells, 3T3-L1 pre-adipocytes, and 489-2.1 multipotent stromal cells--is studied in detail. FND labeling is performed by incubating the cells in a serum-free medium containing 80 μg mL(-1) FND for 4 h. No significant alteration in growth or proliferation of the FND-labeled cells, including the multipotent stromal cells, is observed for up to 8 days...
December 2, 2011: Small
Jens M Kelm, Martin Breitbach, Gregor Fischer, Bernhard Odermatt, Irina Agarkova, Bernd K Fleischmann, Simon P Hoerstrup
Current implantation formats to deliver bone marrow-derived mesenchymal stem cells (MSCs) to the site of myocardial injury resulted only in limited cell retention and integration. As an alternative concept to single cell transplantation, we investigated the fate of cell tracker-labeled syngenic rat MSC microtissue implants, injected into the scar area in a chronic rat myocardial infarction model. Analysis of the explants after 2 and 7 days revealed substantial amounts of the cell tracker within the infarct region...
April 2012: Tissue Engineering. Part A
Kerstin M Galler, Jeffrey D Hartgerink, Adriana C Cavender, Gottfried Schmalz, Rena N D'Souza
Root canal therapy is common practice in dentistry. During this procedure, the inflamed or necrotic dental pulp is removed and replaced with a synthetic material. However, recent research provides evidence that engineering of dental pulp and dentin is possible by using biologically driven approaches. As tissue engineering strategies hold the promise to soon supersede conventional root canal treatment, there is a need for customized scaffolds for stem cell delivery or recruitment. We hypothesize that the incorporation of dental pulp-derived stem cells with bioactive factors into such a scaffold can promote cell proliferation, differentiation, and angiogenesis...
January 2012: Tissue Engineering. Part A
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