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https://www.readbyqxmd.com/read/28173613/illumina-sequencing-based-analysis-of-sediment-bacteria-community-in-different-trophic-status-freshwater-lakes
#1
Yu Wan, Xiaohong Ruan, Yaping Zhang, Rongfu Li
Sediment bacterial community is the main driving force for nutrient cycling and energy transfer in aquatic ecosystem. A thorough understanding of the community's spatiotemporal variation is critical for us to understand the mechanisms of cycling and transfer. Here, we investigated the sediment bacterial community structures and their relations with environmental factors, using Lake Taihu as a model system to explore the dependence of biodiversity upon trophic level and seasonality. To combat the limitations of conventional techniques, we employed Illumina MiSeq Sequencing and LeFSe cladogram to obtain a more comprehensive view of the bacterial taxonomy and their variations of spatiotemporal distribution...
February 7, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28149554/body-mass-index-percent-forced-vital-capacity-respiratory-hospitalization-new-staging-for-idiopathic-pulmonary-fibrosis-patients
#2
Tomoo Kishaba, Hiroaki Nagano, Yuichiro Nei, Shin Yamashiro
BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is relentless progressive interstitial lung disease. Evaluating predictor of mortality for IPF patients is crucial. The aim of this study was to evaluate the serial trend of important indicators of prognosis and create a useful staging method for IPF patients. METHODS: We retrospectively searched medical records, pulmonary function tests (PFTs), and chest high resolution computed tomography (HRCT) scans from January 1, 2008 through June 30, 2015 at our hospital...
December 2016: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28116682/deltas-freshwater-discharge-and-waves-along-the-young-sound-ne-greenland
#3
Aart Kroon, Jakob Abermann, Mette Bendixen, Magnus Lund, Charlotte Sigsgaard, Kirstine Skov, Birger Ulf Hansen
A wide range of delta morphologies occurs along the fringes of the Young Sound in Northeast Greenland due to spatial heterogeneity of delta regimes. In general, the delta regime is related to catchment and basin characteristics (geology, topography, drainage pattern, sediment availability, and bathymetry), fluvial discharges and associated sediment load, and processes by waves and currents. Main factors steering the Arctic fluvial discharges into the Young Sound are the snow and ice melt and precipitation in the catchment, and extreme events like glacier lake outburst floods (GLOFs)...
February 2017: Ambio
https://www.readbyqxmd.com/read/28062612/cardiac-troponin-assays-guide-to-understanding-analytical-characteristics-and-their-impact-on-clinical-care
#4
REVIEW
Fred S Apple, Yader Sandoval, Allan S Jaffe, Jordi Ordonez-Llanos
BACKGROUND: Cardiac troponin I (cTnI) and cardiac troponin T (cTnT) determinations are fixtures in clinical practice and research. Cardiac troponin testing has been the standard of practice for the diagnosis of acute myocardial infarction (AMI), early rule-out, risk stratification, and outcomes assessment in patients presenting with acute coronary syndrome (ACS) and non-ACS myocardial injury. We recognize from reading the literature over the past several years how poorly understood the analytical characteristics are for cTnI and cTnT assays by laboratorians, clinicians, and scientists who use these assays...
January 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28040609/in-situ-observation-of-lubricant-film-formation-in-thr-considering-real-conformity-the-effect-of-diameter-clearance-and-material
#5
D Nečas, M Vrbka, F Urban, J Gallo, I Křupka, M Hartl
The aim of the present study is to provide an analysis of protein film formation in hip joint replacements considering real conformity based on in situ observation of the contact zone. The main attention is focused on the effect of implant nominal diameter, diametric clearance and material. For this purpose, a pendulum hip joint simulator equipped with electromagnetic motors enabling to apply continuous swinging flexion-extension motion was employed. The experimental configuration consists of femoral component (CoCrMo, BIOLOX®forte, BIOLOX®delta) and acetabular cup from optical glass fabricated according to the dimensions of real cups...
December 23, 2016: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28005993/decreased-peak-expiratory-flow-associated-with-muscle-fiber-type-switching-in-spinal-and-bulbar-muscular-atrophy
#6
Shinichiro Yamada, Atsushi Hashizume, Yasuhiro Hijikata, Tomonori Inagaki, Keisuke Suzuki, Naohide Kondo, Kaori Kawai, Seiya Noda, Hirotaka Nakanishi, Haruhiko Banno, Akihiro Hirakawa, Haruki Koike, Katherine Halievski, Cynthia L Jordan, Masahisa Katsuno, Gen Sobue
The aim of this study was to characterize the respiratory function profile of subjects with spinal and bulbar muscular atrophy (SBMA), and to explore the underlying pathological mechanism by comparing the clinical and biochemical indices of this disease with those of amyotrophic lateral sclerosis (ALS). We enrolled male subjects with SBMA (n = 40) and ALS (n = 25) along with 15 healthy control subjects, and assessed their respiratory function, motor function, and muscle strength. Predicted values of peak expiratory flow (%PEF) and forced vital capacity were decreased in subjects with SBMA compared with controls...
2016: PloS One
https://www.readbyqxmd.com/read/27916688/effects-of-repeated-treatment-with-a-delta-opioid-receptor-agonist-knt-127-on-hyperemotionality-in-olfactory-bulbectomized-rats
#7
Leo Gotoh, Akiyoshi Saitoh, Misa Yamada, Hideaki Fujii, Hiroshi Nagase, Mitsuhiko Yamada
We previously demonstrated that a single treatment of a non-peptidic delta opioid receptor agonist, KNT-127, has an antidepressant-like effect in rodents in the forced swim test. Here we evaluated the effect of repeated administration of the potential antidepressant KNT-127 in an olfactory-bulbectomized (OBX) rat model. Male Wistar rats (8-12 weeks old) underwent olfactory bulbectomy. From 14days after surgery each was weighed and administered either KNT-127 (3mgkg(-1)/day), the selective serotonin reuptake inhibitor (SSRI) fluoxetine (10mgkg(-1)/day), or vehicle, daily for 14 days...
December 1, 2016: Behavioural Brain Research
https://www.readbyqxmd.com/read/27915392/sediment-suspension-and-the-dynamic-mechanism-during-storms-in-the-yellow-river-delta
#8
Shuhua Bian, Zjian Hu, Jianqiang Liu, Zichen Zhu
The suspension and hydrodynamic characteristics of the Yellow River Delta during storms were analyzed based on suspended samples obtained using automatic samplers during a storm event in the Yellow River Delta. Synchronous data for winds, waves, and tides were also collected from a nearby station. The results show that under wind speeds of 5-15 m/s and wave heights of 50-150 cm, the suspended content reached 5.7-49.6 kg/m(3), which is 10-100 times higher than that under normal weather conditions. The medium diameter of suspended particles was 1...
December 2016: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/27807273/a-new-mechanism-for-spatial-pattern-formation-via-lateral-and-protrusion-mediated-lateral-signalling
#9
Zena Hadjivasiliou, Ginger L Hunter, Buzz Baum
Tissue organization and patterning are critical during development when genetically identical cells take on different fates. Lateral signalling plays an important role in this process by helping to generate self-organized spatial patterns in an otherwise uniform collection of cells. Recent data suggest that lateral signalling can be mediated both by junctional contacts between neighbouring cells and via cellular protrusions that allow non-neighbouring cells to interact with one another at a distance. However, it remains unclear precisely how signalling mediated by these distinct types of cell-cell contact can physically contribute to the generation of complex patterns without the assistance of diffusible morphogens or pre-patterns...
November 2016: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/27734456/-opioidergic-postconditioning-of-heart-muscle-during-ischemia-reperfusion-injury
#10
Marcin Kunecki, Wojciech Płazak, Tomasz Roleder, Jolanta Biernat, Tomasz Oleksy, Piotr Podolec, Krzysztof Stanisław Gołba
BACKGROUND: Ischemic preconditioning (IPC) and postconditioning (POC) are the novel strategies of attaining cardioprotection against ischemia/reperfusion (I/R) injury. Previous studies suggested the role of opioid pathway, however the class of opioid receptors responsible for this effect in humans remains unknown. The aim of the study was to assess the influence of opioids on simulated I/R injury outcomes in the human myocardium. METHODS: Trabeculae of the human right atrium were electrically driven in organ bath and subjected to simulated I/R injury...
October 13, 2016: Cardiology Journal
https://www.readbyqxmd.com/read/27709074/correlation-of-delta-high-resolution-computed-tomography-hrct-score-with-delta-clinical-variables-in-early-systemic-sclerosis-ssc-patients
#11
Suparaporn Wangkaew, Juntima Euathrongchit, Pittaporn Wattanawittawas, Nuntana Kasitanon
BACKGROUND: The correlation of changes (delta: Δ) of high-resolution computed tomography (HRCT) score with the Δ of other clinical variables has not been well studied. The purpose of this study was to determine the correlation of Δ HRCT score with Δ percent predicted forced vital capacity (%pFVC), Δ modified Rodnan Skin Score (mRSS), Δ erythrocyte sedimentation rate (ESR), and Δ percent of oxygen saturation at room air (%SpO2) in patients with early systemic sclerosis (SSc). METHODS: We used an inception cohort of early-SSc patients seen at the Rheumatology Clinic, Chiang Mai University, Thailand, between January 2010 and June 2014...
August 2016: Quantitative Imaging in Medicine and Surgery
https://www.readbyqxmd.com/read/27684742/production-of-materials-with-spatially-controlled-cross-link-density-via-vat-photopolymerization
#12
Gregory I Peterson, Johanna J Schwartz, Di Zhang, Benjamin M Weiss, Mark A Ganter, Duane W Storti, Andrew J Boydston
We describe an efficient method to produce objects comprising spatially controlled and graded cross-link densities using vat photopolymerization additive manufacturing (AM). Using a commercially available diacrylate-based photoresin, 3D printer, and digital light processing (DLP) projector, we projected grayscale images to print objects in which the varied light intensity was correlated to controlled cross-link densities and associated mechanical properties. Cylinder and bar test specimens were used to establish correlations between light intensities used for printing and cross-link density in the resulting specimens...
October 11, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27624599/the-role-of-regulator-of-g-protein-signaling-4-in-delta-opioid-receptor-mediated-behaviors
#13
Isaac J Dripps, Qin Wang, Richard R Neubig, Kenner C Rice, John R Traynor, Emily M Jutkiewicz
RATIONALE: Regulator of G protein signaling (RGS) proteins act as negative modulators of G protein signaling. RGS4 has been shown to negatively modulate G protein signaling mediated by the delta opioid receptor (DOPr) in vitro. However, the role of RGS4 in modulating DOPr-mediated behaviors in vivo has not been elucidated. OBJECTIVE: The aim of this study was to compare the ability of the DOPr agonist SNC80 to induce DOPr-mediated antinociception, antihyperalgesia, antidepressant-like effects, and convulsions in wild-type and RGS4 knockout mice...
January 2017: Psychopharmacology
https://www.readbyqxmd.com/read/27591356/human-kinetochores-are-swivel-joints-that-mediate-microtubule-attachments
#14
Chris A Smith, Andrew D McAinsh, Nigel J Burroughs
Chromosome segregation is a mechanical process that requires assembly of the mitotic spindle - a dynamic microtubule-based force-generating machine. Connections to this spindle are mediated by sister kinetochore pairs, that form dynamic end-on attachments to microtubules emanating from opposite spindle poles. This bi-orientation generates forces that have been reported to stretch the kinetochore itself, which has been suggested to stabilise attachment and silence the spindle checkpoint. We reveal using three dimensional tracking that the outer kinetochore domain can swivel around the inner kinetochore/centromere, which results in large reductions in intra-kinetochore distance (delta) when viewed in lower dimensions...
September 3, 2016: ELife
https://www.readbyqxmd.com/read/27549033/high-time-resolved-elemental-components-in-fine-and-coarse-particles-in-the-pearl-river-delta-region-of-southern-china-dynamic-variations-and-effects-of-meteorology
#15
Shengzhen Zhou, Perry K Davy, Xuemei Wang, Jason Blake Cohen, Jiaquan Liang, Minjuan Huang, Qi Fan, Weihua Chen, Ming Chang, Travis Ancelet, William J Trompetter
Hourly-resolved PM2.5 and PM10-2.5 samples were collected in the industrial city Foshan in the Pearl River Delta region, China. The samples were subsequently analyzed for elemental components and black carbon (BC). A key purpose of the study was to understand the composition of particulate matter (PM) at high-time resolution in a polluted urban atmosphere to identify key components contributing to extreme PM concentration events and examine the diurnal chemical concentration patterns for air quality management purposes...
August 20, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27468593/the-effect-of-benzocaine-and-ketoprofen-gels-on-pain-during-fixed-orthodontic-appliance-treatment-a-randomised-double-blind-crossover-trial
#16
RANDOMIZED CONTROLLED TRIAL
Ladan Eslamian, Ali Borzabadi-Farahani, Hadi Gholami
AIMS: To compare the analgesic effect of topical benzocaine (5%) and ketoprofen (1.60 mg/mL) after 2 mm activation of 7 mm long delta loops used for maxillary en-masse orthodontic space closure. SUBJECTS AND METHODS: Twenty patients (seven males, 13 females, 15-25 years of age, mean age of 19.5 years) participated in a randomised crossover, double-blind trial. After appliance activation, participants were instructed to use analgesic gels and record pain perception at 2, 6, 24 hours and 2, 3 and 7 days (at 18...
May 2016: Australian Orthodontic Journal
https://www.readbyqxmd.com/read/27456840/the-effects-of-helmet-weight-on-hybrid-iii-head-and-neck-responses-by-comparing-unhelmeted-and-helmeted-impacts
#17
Ron Jadischke, David C Viano, Joe McCarthy, Albert I King
Most studies on football helmet performance focus on lowering head acceleration-related parameters to reduce concussions. This has resulted in an increase in helmet size and mass. The objective of this paper was to study the effect of helmet mass on head and upper neck responses. Two independent test series were conducted. In test series one, 90 pendulum impact tests were conducted with four different headform and helmet conditions: unhelmeted Hybrid III headform, Hybrid III headform with a football helmet shell, Hybrid III headform with helmet shell and facemask, and Hybrid III headform with the helmet and facemask with mass added to the shell (n = 90)...
October 1, 2016: Journal of Biomechanical Engineering
https://www.readbyqxmd.com/read/27448348/dyadic-universal-functions-and-simultaneous-near-field-far-field-regularization-of-elastodynamic-dyadic-green-s-functions-for-3-d-mass-loading-analysis-in-microacoustic-devices
#18
Alireza Baghai-Wadji
Assuming an infinite-domain isotropic elastic medium and a Dirac delta function vector-force excitation, [Formula: see text], spectral domain dyadic Green's functions (DGFs) G for 3-D mass-loading analysis in microacoustic devices have been constructed. Thereby, equivalent inhomogeneous plane-wave (2-D Fourier transform) and homogeneous plane-wave (3-D Fourier transform) representations, G(k1,k2) and G(k1,k2,k3) , respectively, have been obtained in closed form. The construction of G(k1,k2) follows an earlier scheme by utilizing the diagonalization of the governing equations subject to the radiation condition...
October 2016: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
https://www.readbyqxmd.com/read/27385703/stability-of-dronabinol-capsules-when-stored-frozen-refrigerated-or-at-room-temperature
#19
Michael F Wempe, Alan Oldland, Nancy Stolpman, Tyree H Kiser
PURPOSE: Results of a study to determine the 90-day stability of dronabinol capsules stored under various temperature conditions are reported. METHODS: High-performance liquid chromatography (HPLC) with ultraviolet (UV) detection was used to assess the stability of dronabinol capsules (synthetic delta-9-tetrahydrocannabinol [Δ9-THC] mixed with high-grade sesame oil and other inactive ingredients and encapsulated as soft gelatin capsules) that were frozen, refrigerated, or kept at room temperature for three months...
July 15, 2016: American Journal of Health-system Pharmacy: AJHP
https://www.readbyqxmd.com/read/27375969/shapes-of-leaves-with-parallel-venation-modelling-of-the-epipactis-sp-orchidaceae-leaves-with-the-help-of-a-system-of-coupled-elastic-beams
#20
Anna Jakubska-Busse, Maciej Janowicz, Luiza Ochnio, Beata Jackowska-Zduniak
Static properties of leaves with parallel venation, with particular emphasis on the genus EpipactisZinn, 1757 (Orchidaceae, Neottieae) have been modelled with coupled quasi-parallel elastic "beams." The non-linear theory of strongly bended beams have been employed. The resulting boundary-value problem has been solved numerically with the help of the finite-difference method. Possible dislocations resulting in additional Dirac-delta like forces have been take into account. Morphological similarity of the model and real leaves has been obtained...
2016: PeerJ
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