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https://www.readbyqxmd.com/read/28927044/isolation-and-characterization-of-adult-mammary-stem-cells-from-breast-cancer-adjacent-tissues
#1
Ai-Ping Shi, Zhi-Min Fan, Ke-Wei Ma, Yan-Fang Jiang, Lei Wang, Ke-Wei Zhang, Shi-Bo Fu, Ning Xu, Zhi-Ru Zhang
Normal adult mammary stem cells (AMSCs) are promising sources for breast reconstruction, particularly following the resection of breast tumors. However, carcinogenic events can potentially convert normal AMSCs to cancer stem cells, posing a safety concern for the use of AMSCs for clinical tissue regeneration. In the present study, AMSCs and autologous primary breast cancer cells were isolated and compared for their ability to differentiate, their gene expression profile, and their potential to form tumors in vivo...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28926642/a-late-holocene-pollen-record-from-proglacial-oblong-tarn-mount-kenya
#2
Colin J Courtney Mustaphi, Konrad Gajewski, Rob Marchant, Gunhild Rosqvist
High-elevation ecosystems, such as those on Mount Kenya are undergoing significant changes, with accelerated glacial ice losses over the twentieth century creating new space for alpine plants to establish. These ecosystems respond rapidly to climatic variability and within decades of glacial retreat, Afroalpine pioneering taxa stabilize barren land and facilitate soil development, promoting complex patches of alpine vegetation. Periglacial lake sediment records can be used to examine centennial and millennial scale variations in alpine and montane vegetation compositions...
2017: PloS One
https://www.readbyqxmd.com/read/28926380/osseointegrated-transtibial-implants-in-patients-with-peripheral-vascular-disease-a-multicenter-case-series-of-5-patients-with-1-year-follow-up
#3
Robin Atallah, Jiao Jiao Li, William Lu, Ruud Leijendekkers, Jan Paul Frölke, Munjed Al Muderis
BACKGROUND: Osseointegration is an alternative treatment for amputees who are unable to wear or have difficulty wearing a socket prosthesis. Although the majority of limb amputations are due to vascular disease, such amputations have been perceived as a contraindication to osseointegration surgery. We report the outcomes of osseointegrated reconstruction in a series of 5 patients with limb amputation due to peripheral vascular disease. METHODS: Five patients with transtibial amputation and a history of peripheral vascular disease who received an osseointegration implant from 2014 to 2015 were followed for 12 months...
September 20, 2017: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/28926370/photon-counting-computed-tomography-for-vascular-imaging-of-the-head-and-neck-first-in-vivo-human-results
#4
Rolf Symons, Daniel S Reich, Mohammadhadi Bagheri, Tyler E Cork, Bernhard Krauss, Stefan Ulzheimer, Steffen Kappler, David A Bluemke, Amir Pourmorteza
PURPOSE: The purpose of this study was to evaluate image quality of a spectral photon-counting detector (PCD) computed tomography (CT) system for evaluation of major arteries of the head and neck compared with conventional single-energy CT scans using energy-integrating detectors (EIDs). METHODS: In this institutional review board-approved study, 16 asymptomatic subjects (7 men) provided informed consent and received both PCD and EID contrast-enhanced CT scans of the head and neck (mean age, 58 years; range, 46-75 years)...
September 18, 2017: Investigative Radiology
https://www.readbyqxmd.com/read/28926171/biliary-reconstructive-techniques-and-associated-anatomic-variants-in-adult-living-donor-liver-transplants-the-a2all-experience
#5
Talia B Baker, Michael A Zimmerman, Nathan P Goodrich, Benjamin Samstein, Elizabeth A Pomfret, James J Pomposelli, Brenda W Gillespie, Carl L Berg, Jean C Emond, Robert M Merion
INTRODUCTION: Living donor liver transplantation (LDLT) is a technically demanding endeavor, requiring command of the complex anatomy of partial liver grafts. We examined the influence of anatomic variation and reconstruction technique on surgical outcomes and graft survival in the nine-center A2ALL Study. METHODS: Data from 272 adult LDLT recipients (2011-2015) included details on anatomic characteristics and types of intraoperative biliary reconstruction. Associations were tested between reconstruction technique and complications, which included first biliary complication ([BC]; leak, stricture, or biloma) and first vascular complication (hepatic artery thrombosis [HAT] or portal vein thrombosis [PVT])...
September 19, 2017: Liver Transplantation
https://www.readbyqxmd.com/read/28926122/stem-cells-and-differentiated-cells-differ-in-their-sensitivity-to-urine-in-vitro
#6
Marta Pokrywczyńska, Tomasz Kloskowski, Daria Balcerczyk, Monika Buhl, Arkadiusz Jundziłł, Maciej Nowacki, Kaja Męcińska-Jundziłł, Tomasz Drewa
INTRODUCTION: Urinary tract regeneration using tissue engineering is one of the most challenging issues in the field of reconstructive urology. Cells seeded on scaffold are exposed to urine immediately after the implantation. The outcome of urinary bladder regeneration is depended on the ability of these cells to survive, proliferate and regenerate. The aim of this study was to compare a sensitivity of three different cell lines to urine in vitro. MATERIAL AND METHODS: Three different cell lines were isolated from porcine bladder (urothelial cells, UCs and smooth muscle cells, SMCs) and adipose tissue (adipose-derived stem cells, ADSCs)...
September 19, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28926116/a-molar-microwear-texture-analysis-of-pitheciid-primates
#7
Anna J Ragni, Mark F Teaford, Peter S Ungar
Dental microwear textures have been examined for a broad range of extant primates to assess their efficacy for reconstructing diets of fossil species. To date though, no dental microwear texture data have been published for pitheciid molars, despite reported variation in degree of sclerocarpy and, by extension, the fracture properties of foods these platyrrhines eat. While all pitheciids eat hard or tough seeds, Chiropotes and Pithecia have been documented to consume more than Callicebus. In this study, we explored whether measures of molar microwear texture complexity discriminate taxa following variation in reliance upon seeds, and whether dispersion among variables is greatest in Callicebus, which has the most variable diet...
September 19, 2017: American Journal of Primatology
https://www.readbyqxmd.com/read/28925947/a-dynamic-multi-projection-contour-approximating-framework-for-the-3d-reconstruction-of-buildings-by-super-generalized-optical-stereo-pairs
#8
Yiming Yan, Nan Su, Chunhui Zhao, Liguo Wang
In this paper, a novel framework of the 3D reconstruction of buildings is proposed, focusing on remote sensing super-generalized stereo-pairs (SGSPs). As we all know, 3D reconstruction cannot be well performed using nonstandard stereo pairs, since reliable stereo matching could not be achieved when the image-pairs are collected at a great difference of views, and we always failed to obtain dense 3D points for regions of buildings, and cannot do further 3D shape reconstruction. We defined SGSPs as two or more optical images collected in less constrained views but covering the same buildings...
September 19, 2017: Sensors
https://www.readbyqxmd.com/read/28925823/deep-learning-mr-imaging-based-attenuation-correction-for-pet-mr-imaging
#9
Fang Liu, Hyungseok Jang, Richard Kijowski, Tyler Bradshaw, Alan B McMillan
Purpose To develop and evaluate the feasibility of deep learning approaches for magnetic resonance (MR) imaging-based attenuation correction (AC) (termed deep MRAC) in brain positron emission tomography (PET)/MR imaging. Materials and Methods A PET/MR imaging AC pipeline was built by using a deep learning approach to generate pseudo computed tomographic (CT) scans from MR images. A deep convolutional auto-encoder network was trained to identify air, bone, and soft tissue in volumetric head MR images coregistered to CT data for training...
September 19, 2017: Radiology
https://www.readbyqxmd.com/read/28925512/suprafascial-dissection-of-the-pedicled-groin-flap-a-safe-and-practical-approach-to-flap-harvest
#10
Mohamed Abdelrahman, Jonathan Zelken, Ren-Wen Huang, Chung-Chen Hsu, Chih-Hung Lin, Yu-Te Lin, Cheng-Hung Lin
BACKGROUND: The groin flap has been relied upon for more than 4 decades and is well suited for reconstruction of the mutilated hand. Classic groin flap harvest is subfascial and includes the superficial circumflex iliac artery (SCIA). SCIA perforator flaps have shown that one perforator is sufficient to supply a large flap without breaching fascia. Accordingly, we routinely preserve the fascia and rely wholly on the superficial branch of the SCIA, sparing the deep branch. We aim to investigate the safety of suprafascial flap elevation and encourage a paradigm shift in reconstruction with groin flap transfer...
September 19, 2017: Microsurgery
https://www.readbyqxmd.com/read/28925493/repair-and-sensory-reconstruction-of-the-children-s-finger-pulp-defects-with-perforator-pedicled-propeller-flap-in-proper-digital-artery
#11
S-M Feng, A-G Wang, Z-Y Zhang, Q-Q Sun, Y-L Tao, M-M Zhou, Y-J Hao
OBJECTIVE: This study was designed to investigate the clinical effects of adopting perforator pedicled propeller flap in the proper digital artery for treatment and sensory reconstruction of finger pulp defects in children. PATIENTS AND METHODS: Perforator pedicled propeller flap of proper digital arteries in thirty-one fingers from twenty-three children patients were selected for repairing the pulp defects on the 2nd to the 5th fingers. All cases were treated from September 2012 to December 2013...
August 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28925490/multi-detector-spiral-ct-diagnosis-of-common-bile-duct-ampullary-carcinoma
#12
D-Z Zhao, Y Guo, Y-P Sun, H-M Liu, Z Zhang, Q-L Ma, Y-S Wang, C-L Chen
OBJECTIVE: The aim of this project was to study the imaging characteristics of multi-detector CT (MDCT) in different types of malignant tumor in the common bile duct ampulla. PATIENTS AND METHODS: We examined 30 cases of pancreatic head cancer, 35 of terminal cholangiocarcinoma, 26 of ampullary carcinoma, and 40 of benign lesions, all confirmed by pathology. We used 64-slice spiral CT plain scan and multi-phase enhanced scan with multi-planar reconstruction (MPR) and curved planar reconstruction (CPR) post-processing to obtain three-dimensional images...
August 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28925270/-reconstruction-of-the-posterior-and-central-compartment-with-the-prolift-posterior-cohort-study-with-a-5-year-follow-up
#13
K Brányik, L Krofta, P Kraus, M Krčmář, O Gojiš, I Urbánková, J Feyereisl
OBJECTIVE: To analyse the results of the long-term prospective follow-up study of vaginal prolapse reconstructed using a Prolift Posterior. STUDY DESIGN: Prospective, cohort study. SETTING: Institute for the Care of Mother and Child, Prague. METHODS: Over a 5-year period (I/2006 - XII/2011) we prospectively followed a cohort of patients with posterior vaginal wall defect who underwent surgical reconstruction using a monofilament polypropylene implant Prolift Posterior (Gynecare, Ethicon, Inc...
2017: Ceská Gynekologie
https://www.readbyqxmd.com/read/28925086/optimizing-hepatic-venous-outflow-reconstruction-for-hepatic-vein-stenosis-with-indwelling-stent-in-living-donor-liver-retransplantation
#14
Takanobu Shigeta, Seisuke Sakamoto, Kengo Sasaki, Hajime Uchida, Soichi Narumoto, Akinari Fukuda, Mureo Kasahara
The patient was a boy of 7 years and 5 months of age, who underwent LDLT for acute liver failure at 10 months of age. HV stent placement was performed 8 months after LDLT because of intractable HV stenosis. At 7 years of age, his liver function deteriorated due to chronic rejection. The patient therefore underwent living donor liver retransplantation from his father. The HV was transected with the stent in situ. The IVC was resected due to stenosis. The pericardial cavity was opened and detached around the IVC to elongate the IVC...
September 18, 2017: Pediatric Transplantation
https://www.readbyqxmd.com/read/28924879/a-systematic-review-and-meta-analysis-of-timing-and-outcome-of-intestinal-failure-surgery-in-patients-with-enteric-fistula
#15
Fleur E E de Vries, Jasper J Atema, Oddeke van Ruler, Carolynne J Vaizey, Mireille J Serlie, Marja A Boermeester
BACKGROUND: The timing of intestinal failure (IF) surgery has changed. Most specialized centers now recommend postponing reconstructive surgery for enteric fistula and emphasize that abdominal sepsis has to be resolved and the patient's condition improved. Our aim was to study the outcome of postponed surgery, to identify risk factors for recurrence and mortality, and to define more precisely the optimal timing of reconstructive surgery. METHODS: PubMed, Embase, and the Cochrane Library were systematically reviewed on the outcomes of reconstructive IF surgery (fistula recurrence, mortality, morbidity, hernia recurrence, total closure, enteral autonomy)...
September 18, 2017: World Journal of Surgery
https://www.readbyqxmd.com/read/28924872/-alzheimer-s-disease-from-auguste-deter-to-the-present-progress-disappointments-and-open-questions
#16
REVIEW
Johannes Pantel
AIM: The present article aims to provide a short overview of the discovery history, conceptual development, as well as on current neurobiological and pharmacological research questions in the field of Alzheimer's disease (AD). In view of the long hoped for but so far unachieved therapeutic breakthrough, this also includes a critical reflection of current research paradigms. MATERIAL AND METHODS: Starting from the first case report described by Alois Alzheimer in 1906, the historical impact of his seminal discovery is reconstructed...
September 18, 2017: Zeitschrift Für Gerontologie und Geriatrie
https://www.readbyqxmd.com/read/28924863/evaluation-of-deep-myometrial-invasion-in-endometrial-cancer-patients-is-dual-energy-ct-an-option
#17
Stefania Rizzo, Marco Femia, Davide Radice, Maria Del Grande, Dorella Franchi, Daniela Origgi, Valentina Buscarino, Alberto Mauro, Massimo Bellomi
OBJECTIVES: Assessment of deep (>50%) myometrial invasion by dual-energy CT (DECT) and Trans-Vaginal US (TVUS) in patients with endometrial cancer. METHODS: We retrospectively enrolled patients with endometrial cancer who underwent DECT and TVUS for pre-surgical staging. Three sets of images were evaluated: 70 keV (routine CT images), 50 keV, and iodine-water reconstructions. The gold standard was pathology after surgery. The agreement between the different imaging modalities and the gold standard was estimated...
September 18, 2017: La Radiologia Medica
https://www.readbyqxmd.com/read/28924721/carcinoma-in-the-remnant-stomach-during-long-term-follow-up-after-distal-gastrectomy-for-gastric-cancer-analysis-of-cumulative-incidence-and-associated-risk-factors
#18
Takaaki Hanyu, Atsuhiro Wakai, Takashi Ishikawa, Hiroshi Ichikawa, Hitoshi Kameyama, Toshifumi Wakai
BACKGROUND: The number of patients with remnant gastric cancer following resection of gastric cancer may increase. The aims of this study were to investigate the development of remnant gastric cancer after distal gastrectomy for gastric cancer and to examine its cumulative incidence, clinicopathological characteristics, and risk factors. METHODS: We examined 437 patients with relapse-free survival for 5 years or more after distal gastrectomy with Billroth I reconstruction for gastric cancer performed between 1985 and 2005...
September 18, 2017: World Journal of Surgery
https://www.readbyqxmd.com/read/28924692/sediment-core-data-reconstruct-the-management-history-and-usage-of-a-heavily-modified-urban-lake-in-berlin-germany
#19
Robert Ladwig, Lena Heinrich, Gabriel Singer, Michael Hupfer
Urban surface waters face several stressors associated with industry and urban water management. Over much of the past century, the wastewater treatment in Berlin, Germany, relied on inefficient sewage farms, which resulted in severe eutrophication and sediment contamination in the recipient surface waterbodies. A prominent example is Lake Tegel, where a multitude of management measures were applied in the last decades for the purpose of ecosystem restoration. In this study, we analyzed sediment cores of three lakes with X-ray fluorescence spectroscopy: Lake Tegel, Lake Großer Wannsee, which is environmentally similar but has a different management history, and Lake Userin, which serves as a reference located in a nature protection area...
September 18, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28924672/in-situ-super-resolution-imaging-of-genomic-dna-with-oligostorm-and-oligodna-paint
#20
Brian J Beliveau, Alistair N Boettiger, Guy Nir, Bogdan Bintu, Peng Yin, Xiaowei Zhuang, C-Ting Wu
OligoSTORM and OligoDNA-PAINT meld the Oligopaint technology for fluorescent in situ hybridization (FISH) with, respectively, Stochastic Optical Reconstruction Microscopy (STORM) and DNA-based Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) to enable in situ single-molecule super-resolution imaging of nucleic acids. Both strategies enable ≤20 nm resolution and are appropriate for imaging nanoscale features of the genomes of a wide range of species, including human, mouse, and fruit fly (Drosophila)...
2017: Methods in Molecular Biology
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