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https://www.readbyqxmd.com/read/29160568/nerve-detection-using-optical-spectroscopy-an-evaluation-in-four-different-models-in-human-and-swine-in-vivo-and-post-mortem
#1
Gerrit C Langhout, Torre M Bydlon, Marjolein van der Voort, Manfred Müller, Jeroen Kortsmit, Gerald Lucassen, Andrea J R Balthasar, Geert-Jan van Geffen, Thorsten Steinfeldt, Henricus J C M Sterenborg, Benno H W Hendriks, Theo J M Ruers
OBJECTIVE: Identification of peripheral nerve tissue is crucial in both surgery and regional anesthesia. Recently, optical tissue identification methods are presented to facilitate nerve identification in transcutaneous procedures and surgery. Optimization and validation of such techniques require large datasets. The use of alternative models to human in vivo, like human post mortem, or swine may be suitable to test, optimize and validate new optical techniques. However, differences in tissue characteristics and thus optical properties, like oxygen saturation and tissue perfusion are to be expected...
November 21, 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/29126388/yrna-expression-predicts-survival-in-bladder-cancer-patients
#2
Yuri Tolkach, Anna Franziska Stahl, Eva-Maria Niehoff, Chenming Zhao, Glen Kristiansen, Stefan Cajetan Müller, Jörg Ellinger
BACKGROUND: Non-coding RNAs play an important role in human carcinogenesis. YRNAs (Ro-associated Y), a novel class of non-coding RNAs, have been identified as biomarker in various malignancies, but remain to be studied in urinary bladder cancer (BCA) patients. METHODS: The expression of all four YRNAs (RNY1, RNY3, RNY4, RNY5) was determined in archival BCA (urothelial carcinoma, n = 88) and normal urothelial bladder (n = 30) tissues using quantitative real-time PCR...
November 10, 2017: BMC Cancer
https://www.readbyqxmd.com/read/29109262/myoanatomy-of-the-velvet-worm-leg-revealed-by-laboratory-based-nanofocus-x-ray-source-tomography
#3
Mark Müller, Ivo de Sena Oliveira, Sebastian Allner, Simone Ferstl, Pidassa Bidola, Korbinian Mechlem, Andreas Fehringer, Lorenz Hehn, Martin Dierolf, Klaus Achterhold, Bernhard Gleich, Jörg U Hammel, Henry Jahn, Georg Mayer, Franz Pfeiffer
X-ray computed tomography (CT) is a powerful noninvasive technique for investigating the inner structure of objects and organisms. However, the resolution of laboratory CT systems is typically limited to the micrometer range. In this paper, we present a table-top nanoCT system in conjunction with standard processing tools that is able to routinely reach resolutions down to 100 nm without using X-ray optics. We demonstrate its potential for biological investigations by imaging a walking appendage of Euperipatoides rowelli, a representative of Onychophora-an invertebrate group pivotal for understanding animal evolution...
November 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29107291/absence-of-the-kinase-s6k1-mimics-the-effect-of-chronic-endurance-exercise-on-glucose-tolerance-and-muscle-oxidative-stress
#4
C Binsch, T Jelenik, A Pfitzer, M Dille, S Müller-Lühlhoff, S Hartwig, S Karpinski, S Lehr, D G Kabra, A Chadt, M Roden, H Al-Hasani, T R Castañeda
OBJECTIVE: Ribosomal protein S6 Kinase-1 (S6K1) has been linked to resistance exercise-mediated improvements in glycemia. We hypothesized that S6K1 may also play a role in regulating glycemic control in response to endurance exercise training. METHODS: S6k1-knockout (S6K1KO) and WT mice on a 60 cal% high-fat diet were trained for 4 weeks on treadmills, metabolically phenotyped, and compared to sedentary controls. RESULTS: WT mice showed improved glucose tolerance after training...
November 2017: Molecular Metabolism
https://www.readbyqxmd.com/read/29054084/occurrence-and-levels-of-persistent-organic-pollutants-pops-in-farmed-and-wild-marine-fish-from-tanzania-a-pilot-study
#5
Eliezer Brown Mwakalapa, Aviti John Mmochi, Mette Helen Bjorge Müller, Robinson Hammerthon Mdegela, Jan Ludvig Lyche, Anuschka Polder
In 2016, farmed and wild milkfish (Chanos chanos) and mullet (Mugil cephalus) from Tanzania mainland (Mtwara) and Zanzibar islands (Pemba and Unguja) were collected for analyses of persistent organic pollutants (POPs). Fish livers were analysed for organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), brominated flame retardants (BFRs). Muscle tissue was used for analyses of perfluoroalkyl substances (PFASs). The major contaminant was p,p'-DDE. The highest p,p'-DDE concentration was found in wild milkfish from Mtwara (715...
September 26, 2017: Chemosphere
https://www.readbyqxmd.com/read/29045997/the-influence-of-bis-monitoring-on-sedative-dose-in-pediatric-patients-undergoing-open-muscle-biopsies-a-randomized-controlled-trial
#6
Eva Tschiedel, Oliver Müller, Adela Della Marina, Ursula Felderhoff-Müser, Christian Dohna-Schwake
Background The use of Propofol and Remifentanil for analgosedation in children is common and safe. For sedation monitoring clinical scores as Comfort Score (CS) as well as bispectral index (BIS) are frequently applied. The impact of BIS for sedation monitoring in pediatric patients is still under debate. This prospective study aims to investigate whether dual sedation monitoring of CS and BIS compared with monitoring of CS alone during muscle biopsies in children can reduce sedative doses, reduce awakening time and prevent complications...
October 18, 2017: Klinische Pädiatrie
https://www.readbyqxmd.com/read/29034984/sensory-quality-and-fatty-acid-content-of-springbok-antidorcas-marsupialis-meat-influence-of-farm-location-and-gender
#7
Jeannine Neethling, Magdalena Muller, Marieta van der Rijst, Louwrens C Hoffman
BACKGROUND: Springbok are harvested for meat production irrespective of farm location or gender from which the meat is derived. This study investigated the influence of farm location (three farms containing different vegetation types) and gender on the sensory quality of springbok longissimus thoracis et lumborum muscle. The sensory profile (aroma, flavour and texture) was determined by descriptive sensory analysis, in addition to determining the physical meat quality, proximate and fatty acid composition...
October 16, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29032316/fiber-reinforced-silicone-for-tracheobronchial-stents-an-experimental-study
#8
Samanta Bianchi Vearick, Kétner Bendo Demétrio, Rogério Gastal Xavier, Alexandre Heitor Moreschi, André Frotta Muller, Paulo Roberto Stefani Sanches, Luis Alberto Loureiro Dos Santos
A trachea is a tubular structure composed of smooth muscle that is reinforced with cartilage rings. Some diseases can cause sagging in smooth muscle and cartilaginous tissue. The end result is reduction (narrowing) of the trachea diameter. A solution to this problem is the use of tracheal stents, which are small tubular devices made of silicone. One is inserted into the trachea to prevent or correct its constriction. The purpose of tracheal stent use is to maintain cartilage support that would otherwise be lost in the airway...
October 9, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/29026936/a-comparative-study-of-full-thickness-blepharotomy-versus-transconjunctival-eyelid-lengthening-in-the-correction-of-upper-eyelid-retraction-in-graves-orbitopathy
#9
Allan C Pieroni Gonçalves, Thiago Nogueira, Ana Carolina Arato Gonçalves, Luzia Diegues Silva, Suzana Matayoshi, Mário L R Monteiro
BACKGROUND: The study was designed to compare the outcome of full-thickness blepharotomy and transconjunctival eyelid lengthening in the correction of upper eyelid retraction (UER) in patients with Graves' orbitopathy (GO). METHODS: This is a prospective randomized interventional study. Following ophthalmic examination, determination of the ocular surface disease index (OSDI) and photography, 27 patients with UER were randomly assigned to either graded full-thickness blepharotomy (G1) or transconjunctival Müller muscle recession and graded disinsertion of the levator palpebrae superioris muscle (G2)...
October 12, 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/29025901/effects-of-age-and-exercise-training-on-coronary-microvascular-smooth-muscle-phenotype-and-function
#10
Judy M Muller-Delp, Kazuki Hotta, Bei Chen, Bradley Jon Behnke, Joshua J Maraj, Michael D Delp, Tiffani R Lucero, Jeremy A Bramy, David B Alarcon, Hannah E Morgan, Morgan R Cowan, Anthony D Haynes
Coronary microvascular function and blood flow responses during acute exercise are impaired in the aged heart, but can be restored by exercise training. Coronary microvascular resistance is directly dependent on vascular smooth muscle function in coronary resistance arterioles; therefore, we hypothesized that age impairs contractile function and alters the phenotype of vascular smooth muscle in coronary arterioles. We further hypothesized that exercise training restores contractile function and reverses age-induced phenotypic alterations of arteriolar smooth muscle...
October 12, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/29024298/detailed-evidence-for-an-unparalleled-interaction-mode-between-calmodulin-and-orai-proteins
#11
Lukas Traxler, Petr Rathner, Marc Fahrner, Michael Stadlbauer, Felix Faschinger, Tatsiana Charnavets, Norbert Müller, Christoph Romanin, Peter Hinterdorfer, Hermann J Gruber
Calmodulin (CaM) binds most of its targets by wrapping around an amphipathic α-helix. The N-terminus of Orai proteins contains a conserved CaM-binding segment but the binding mechanism has been only partially characterized. Here, microscale thermophoresis (MST), surface plasmon resonance (SPR), and atomic force microscopy (AFM) were employed to study the binding equilibria, the kinetics, and the single-molecule interaction forces involved in the binding of CaM to the conserved helical segments of Orai1 and Orai3...
October 11, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28960914/cpap-and-epap-elicit-similar-lung-deflation-in-a-non-equivalent-mode-in-gold-3-4-copd-patients
#12
Paulo de Tarso Müller, Gustavo Christofoletti, Rodrigo Koch, João Henrique Zardetti Nogueira, Luiz Armando Pereira Patusco, Gaspar Rogério Chiappa
INTRODUCTION: Lung hyperinflation is associated with inspiratory muscle strength reduction, nocturnal desaturation, dyspnea, altered cardiac function and poor exercise capacity in advanced COPD. OBJECTIVES: We investigated the responses of inspiratory capacity (IC) and inspiratory muscle strength (PImax), comparing continuous positive airway pressure (CPAP) and expiratory positive airway pressure (EPAP) with the main hypothesis that there would be similar effects on lung deflation...
September 28, 2017: Clinical Respiratory Journal
https://www.readbyqxmd.com/read/28960717/retrospective-evaluation-of-155-adult-equids-and-21-foals-with-tetanus-in-western-northern-and-central-europe-2000-2014-part-1-description-of-history-and-clinical-evolution
#13
Gaby van Galen, Claude Saegerman, Joke Rijckaert, Helene Amory, Lara Armengou, Barbora Bezdekova, Inge Durie, Rikke Findshøj Delany, Nathalie Fouché, Laura Haley, Michael Hewetson, Rene van den Hoven, Anna Kendall, Fernando Malalana, Jessika Muller Cavalleri, Tresemiek Picavet, Katja Roscher, Denis Verwilghen, Meret Wehrli Eser, Cornélie Westermann, Tim Mair
OBJECTIVE: To describe clinical data of hospitalized adult equids and foals with tetanus. DESIGN: Multicenter retrospective study (2000-2014). SETTING: Twenty Western, Northern, and Central European university teaching hospitals and private referral centers. ANIMALS: One hundred fifty-five adult equids (>6 months) and 21 foals (<6 months) with tetanus. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: Information on geographic, annual and seasonal data, demographic- and management-related data, clinical history, clinical examination and blood analysis on admission, complications, treatments, and outcomes were described and statistically compared between adults and foals...
September 28, 2017: Journal of Veterinary Emergency and Critical Care
https://www.readbyqxmd.com/read/28959214/adrenergic-stress-protection-of-human-ips-cell-derived-cardiomyocytes-by-fast-kv7-1-recycling
#14
Ilaria Piccini, Edda Fehrmann, Stefan Frank, Frank U Müller, Boris Greber, Guiscard Seebohm
The fight-or-flight response (FFR), a physiological acute stress reaction, involves positive chronotropic and inotropic effects on heart muscle cells mediated through β-adrenoceptor activation. Increased systolic calcium is required to enable stronger heart contractions whereas elevated potassium currents are to limit the duration of the action potentials and prevent arrhythmia. The latter effect is accomplished by an increased functional activity of the Kv7.1 channel encoded by KCNQ1. Current knowledge, however, does not sufficiently explain the full extent of rapid Kv7...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28940796/gene-activated-tissue-grafts-for-sustained-bone-morphogenetic-protein-2-delivery-and-bone-engineering-is-muscle-with-fascia-superior-to-muscle-and-fat
#15
Bin Ren, Volker M Betz, Christian Thirion, Michael Salomon, Volkmar Jansson, Peter E Müller, Oliver B Betz
BACKGROUND: Previously, we have presented an expedited strategy for sustained delivery of BMP-2 to bone lesions based on the implantation of gene activated fat and muscle fragments. The aim of the present in vitro experiments was to evaluate the potential of muscle with fascia as a BMP-2 delivering osteo-regenerative implant in comparison to fat tissue and muscle alone. METHODS: Subcutaneous fat, muscle and muscle with fascia were harvested from Fischer 344 rats...
September 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28939892/neurons-and-a-sensory-organ-in-the-pedipalps-of-male-spiders-reveal-that-it-is-not-a-numb-structure
#16
Lenka Sentenská, Carsten H G Müller, Stano Pekár, Gabriele Uhl
The primary function of male copulatory organs is depositing spermatozoa directly into the female reproductive tract. Typical male copulatory organs are sensorily active. This is in contrast to the copulatory organs of male spiders (i.e. palpal bulbi), which have been assumed to lack nerves and muscles until recently. Neurons have been found within the bulbus of the spider Hickmania troglodytes, a taxon basal to all Neocribellata. We provide the first evidence for neurons and an internalized multi-sensillar sensory organ in the bulbus of an entelegyne spider (Philodromus cespitum)...
September 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28914711/change-in-eyelid-position-following-muller-s-muscle-conjunctival-resection-with-a-standard-versus-variable-resection-length
#17
Daniel B Rootman, Kunal R Sinha, Robert A Goldberg
PURPOSE: This study compares the use of a standard 7 mm resection length to a variable 4:1 ratio of resection length to desired elevation nomogram when performing Muller's muscle conjunctival resection surgery. METHODS: In this cross-sectional case control study, 2 groups were defined. The first underwent Muller's muscle conjunctival resection surgery with a standard 7 mm resection length and the second underwent the same surgery with a variable resection length determined by a 4:1 ratio of resection length to desired elevation nomogram...
September 12, 2017: Ophthalmic Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28904224/targeted-inhibition-of-gq-signaling-induces-airway-relaxation-in-mouse-models-of-asthma
#18
Michaela Matthey, Richard Roberts, Alexander Seidinger, Annika Simon, Ralf Schröder, Markus Kuschak, Suvi Annala, Gabriele M König, Christa E Müller, Ian P Hall, Evi Kostenis, Bernd K Fleischmann, Daniela Wenzel
Obstructive lung diseases are common causes of disability and death worldwide. A hallmark feature is aberrant activation of Gq protein-dependent signaling cascades. Currently, drugs targeting single G protein (heterotrimeric guanine nucleotide-binding protein)-coupled receptors (GPCRs) are used to reduce airway tone. However, therapeutic efficacy is often limited, because various GPCRs contribute to bronchoconstriction, and chronic exposure to receptor-activating medications results in desensitization. We therefore hypothesized that pharmacological Gq inhibition could serve as a central mechanism to achieve efficient therapeutic bronchorelaxation...
September 13, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28873654/the-truth-is-in-the-isotopes-authenticating-regionally-unique-south-african-lamb
#19
Sara W Erasmus, Magdalena Muller, Mike Butler, Louwrens C Hoffman
Stable isotope ratios ((13)C/(12)C and (15)N/(14)N) of South African lambs from different regions were measured by isotope ratio mass spectrometry (IRMS). Homogenised and defatted meat of the Longissimus lumborum muscle was assessed. The Rûens and Hantam Karoo regions had the lowest (P≤0.05) δ(13)C values related to the presence of C3 plants (lucerne and Karoo bushes, respectively). The Northern Karoo, Namibia and Bushmanland had the highest δ(13)C values likely due to a high proportion of dietary C4 grass species...
January 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/28857984/levator-aponeurosis-and-muller-muscle-plication-reinforced-with-levator-sheath-advancement-for-blepharoptosis-correction
#20
Jin Suk Byun, Kun Hwang, Sang Yun Lee, Hak Tae Kim, Kenneth Kim
The authors innovated the levator aponeurosis and Muller muscle plication reinforced with levator sheath advancement (AMPSA) for blepharoptosis correction. The orbital septum was opened 1 mm above its fusion with the levator aponeurosis. The preaponeurotic fat was retracted and the thickened part of the levator sheath was identified. Two plication sutures were made: medial suture at the medial border of the pupil and lateral between the lateral border of the pupil and the lateral limbus. A needle with 6-0 nylon thread first bit the tarsal plate approximately 1 mm below its upper border, then bit the levator aponeurosis and the Muller muscle together at 3 to 6 mm above the upper border of the tarsal plate...
October 2017: Journal of Craniofacial Surgery
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