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Clostridium ramosum

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https://www.readbyqxmd.com/read/28535609/synthesis-of-%C3%AE-galactooligosaccharide-using-bifidobacterial-%C3%AE-galactosidase-purified-from-recombinant-escherichia-coli
#1
So Young Oh, So Youn Youn, Myung Soo Park, Hyoung Geun Kim, Nam In Baek, Zhi Peng Li, Geun Eog Ji
Galactooligosaccharides (GOS) are known to be selectively utilized by Bifidobacterium, which can bring about healthy changes of the composition of intestinal microflora. In this study, β-GOS are synthesized using bifidobacterial β-galactosidase(G1) purified from recombinant E. coli with a high GOS yield and with high productivity and enhanced bifidogenic activity. The purified recombinant G1 showed maximum production of β-GOS at pH 8.5 and 45°C. A matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis of the major peaks of the produced β-GOS showed MW of 527 and 689, indicating the synthesis of β-GOS at degrees of polymerization (DP) of 3 and DP4, respectively...
May 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28302207/-a-preliminary-analysis-of-changes-in-composition-of-intestinal-microbiota-during-infancy-using-polymerase-chain-reaction-denaturing-gradient-gel-electrophoresis
#2
Shan-Shan Li, Jun-Jie Miao, Zi-Xi Wu, Jian-Rong Yao, Ming Li, Qian Yu, Fang He
OBJECTIVE: To investigate the composition of bacteria in the stools of infants and the colonization of intestinal microbiota during infancy. METHODS: Fresh stools were collected from 15 healthy infants at 0, 2, 4, 7, 10, 14, and 28 days and 3, 6, and 12 months after birth. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was used to analyze the composition of intestinal microbiota, perform sequencing of dominant bacteria, and to analyze the changes in the composition of intestinal microbiota during infancy...
March 2017: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/27058556/the-intestinal-microbiota-in-metabolic-disease
#3
REVIEW
Anni Woting, Michael Blaut
Gut bacteria exert beneficial and harmful effects in metabolic diseases as deduced from the comparison of germfree and conventional mice and from fecal transplantation studies. Compositional microbial changes in diseased subjects have been linked to adiposity, type 2 diabetes and dyslipidemia. Promotion of an increased expression of intestinal nutrient transporters or a modified lipid and bile acid metabolism by the intestinal microbiota could result in an increased nutrient absorption by the host. The degradation of dietary fiber and the subsequent fermentation of monosaccharides to short-chain fatty acids (SCFA) is one of the most controversially discussed mechanisms of how gut bacteria impact host physiology...
April 6, 2016: Nutrients
https://www.readbyqxmd.com/read/26546506/septic-arthritis-due-to-clostridium-ramosum
#4
Antonio García-Jiménez, Núria Prim, Xavier Crusi, Natividad Benito
OBJECTIVES: Clostridium species are anaerobic bacilli that are rarely reported as etiologic agents of infectious arthritis. Previous cases of arthritis caused by Clostridium ramosum have not been reported. We describe the first 2 cases of C. ramosum arthritis. METHODS: We reviewed the etiology of arthritis in our hospital during the previous 15 years. RESULTS: Both patients had underlying immunocompromising conditions and their infections involved a joint with preexisting disease: patient 1 had rheumatic arthritis and a prosthetic joint; patient 2, chronic renal failure on dialysis and hip osteoarthritis...
April 2016: Seminars in Arthritis and Rheumatism
https://www.readbyqxmd.com/read/26293474/isolation-of-genetically-tractable-most-wanted-bacteria-by-metaparental-mating
#5
Páraic Ó Cuív, Wendy J Smith, Sian Pottenger, Sriti Burman, Erin R Shanahan, Mark Morrison
Metagenomics has rapidly advanced our inventory and appreciation of the genetic potential inherent to the gut microbiome. However it is widely accepted that two key constraints to further genetic dissection of the gut microbiota and host-microbe interactions have been our inability to recover new isolates from the human gut, and the paucity of genetically tractable gut microbes. To address this challenge we developed a modular RP4 mobilisable recombinant vector system and an approach termed metaparental mating to support the rapid and directed isolation of genetically tractable fastidious gut bacteria...
2015: Scientific Reports
https://www.readbyqxmd.com/read/25271283/clostridium-ramosum-promotes-high-fat-diet-induced-obesity-in-gnotobiotic-mouse-models
#6
Anni Woting, Nora Pfeiffer, Gunnar Loh, Susanne Klaus, Michael Blaut
UNLABELLED: The intestines of obese humans and mice are enriched with Erysipelotrichi, a class within the Firmicutes. Clostridium ramosum, a member of the Erysipelotrichi, is associated with symptoms of the metabolic syndrome in humans. To clarify the possible obesogenic potential of this bacterial species and to unravel the underlying mechanism, we investigated the role of C. ramosum in obesity development in gnotobiotic mice. Mice were associated with a simplified human intestinal (SIHUMI) microbiota of eight bacterial species, including C...
2014: MBio
https://www.readbyqxmd.com/read/24813985/antibacterial-activity-against-clostridium-genus-and-antiradical-activity-of-the-essential-oils-from-different-origin
#7
Miroslava Kačániová, Nenad Vukovič, Elena Horská, Ivan Salamon, Alica Bobková, Lukáš Hleba, Martina Fiskelová, Alexander Vatľák, Jana Petrová, Marek Bobko
In the present study, the antimicrobial and antiradical activities of 15 essential oils were investigated. The antimicrobial activities were determined by using agar disc diffusion and broth microdilution methods against Clostridium genus and antioxidant properties of essential oils by testing their scavenging effect on DPPH radicals activities. We determined the antibacterial activity of Clostridium butyricum, Clostridium hystoliticum, Clostridium intestinale, Clostridium perfringens and Clostridium ramosum...
2014: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/24283763/clostridium-ramosum-bacteremia-case-report-and-literature-review
#8
REVIEW
Joseph D Forrester, David A Spain
BACKGROUND: Clostridium ramosum is a common enteric anaerobe but infrequently also a cause of pathologic infection. METHODS: Case report and literature review. RESULTS: We reviewed 12 case reports describing infection with C. ramosum. When pathogenic, C. ramosum is cultured most commonly from the inner ear, anaerobic blood samples, or abscesses. Patients with such infections fall into two demographic groups, consisting of young children with ear infections or immunocompromised adults with bacteremia...
June 2014: Surgical Infections
https://www.readbyqxmd.com/read/24179346/non-autoimmune-hemolytic-anemia-with-clostridium-ramosum-bacteremia
#9
Aref Al-Kali, Alok Oswal, Arafat Tfayli
No abstract text is available yet for this article.
2008: Clinical Medicine. Case Reports
https://www.readbyqxmd.com/read/23877700/comparative-in-vitro-activities-of-smt19969-a-new-antimicrobial-agent-against-clostridium-difficile-and-350-gram-positive-and-gram-negative-aerobic-and-anaerobic-intestinal-flora-isolates
#10
Ellie J C Goldstein, Diane M Citron, Kerin L Tyrrell, C Vreni Merriam
The comparative in vitro activity of SMT19969, a novel, narrow-spectrum, nonabsorbable agent, was studied against 50 ribotype-defined Clostridium difficile strains, 174 Gram-positive and 136 Gram-negative intestinal anaerobes, and 40 Gram-positive aerobes. SMT19969 was one dilution more active against C. difficile isolates (MIC range, 0.125 to 0.5 μg/ml; MIC90, 0.25 μg/ml), including ribotype 027 strains, than fidaxomicin (range, 0.06 to 1 μg/ml; MIC90, 0.5 μg/ml) and two to six dilutions lower than either vancomycin or metronidazole...
October 2013: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/23183985/flx-pyrosequencing-analysis-of-the-effects-of-the-brown-algal-fermentable-polysaccharides-alginate-and-laminaran-on-rat-cecal-microbiotas
#11
Choa An, Takashi Kuda, Takahiro Yazaki, Hajime Takahashi, Bon Kimura
Edible brown algae are used as major food material in Far East Asian countries, particularly in South Korea and Japan. They contain fermentable dietary fibers, alginic acid (uronic acid polymer) and laminaran (β-1,3-glucan), that are fermented into organic acids by intestinal bacteria. To clarify the effect of edible algae on the intestinal environment, the cecal microbiotas of rats fed diets containing no dietary fiber (control) or 2% (wt/wt) sodium alginate or laminaran for 2 weeks were analyzed using FLX amplicon pyrosequencing with bar-coded primers targeting the bacterial 16S rRNA gene...
February 2013: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/22838224/comparison-of-the-antibacterial-effect-of-modified-3-mix-paste-versus-ultrapex-over-anaerobic-microorganisms-from-infected-root-canals-of-primary-teeth-an-in-vitro-study
#12
COMPARATIVE STUDY
N Velasco-Loera, Y De Alba-Vazquez, A Garrocho-Rangel, A M Gonzalez-Amaro, H Flores-Reyes, A J Pozos-Guillen
OBJECTIVE: The aim of this study was to evaluate in vitro the antimicrobial efficacy of a modified 3-mix paste and to compare it with an iodoform paste (Ultrapex) against anaerobic microorganisms isolated from root canals of infected or necrotic primary teeth. STUDY DESIGN: An in vitro experimental assay was performed over isolated and identified anaerobic microorganisms of 21 samples, in order to compare the antimicrobial ability of both root canal filling materials, using a disc-diffusion method...
2012: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/22634331/evaluation-of-impact-of-exposure-of-sudan-azo-dyes-and-their-metabolites-on-human-intestinal-bacteria
#13
Hongmiao Pan, Jinhui Feng, Gui-Xin He, Carl E Cerniglia, Huizhong Chen
Sudan azo dyes are banned for food usage in most countries, but they are illegally used to maintain or enhance the color of food products due to low cost, bright staining, and wide availability of the dyes. In this report, we examined the toxic effects of these azo dyes and their potential reduction metabolites on 11 prevalent human intestinal bacterial strains. Among the tested bacteria, cell growth of 2, 3, 5, 5, and 1 strains was inhibited by Sudan I, II, III, IV, and Para Red, respectively. At the tested concentration of 100 μM, Sudan I and II inhibited growth of Clostridium perfringens and Lactobacillus rhamnosus with decrease of growth rates from 14 to 47%...
August 2012: Anaerobe
https://www.readbyqxmd.com/read/22290948/comparative-in-vitro-activities-of-lff571-against-clostridium-difficile-and-630-other-intestinal-strains-of-aerobic-and-anaerobic-bacteria
#14
COMPARATIVE STUDY
Diane M Citron, Kerin L Tyrrell, C Vreni Merriam, Ellie J C Goldstein
The in vitro activities of LFF571, a novel analog of GE2270A that inhibits bacterial growth by binding with high affinity for protein synthesis elongation factor Tu, fidaxomicin, and 10 other antimicrobial agents were determined against 50 strains of Clostridium difficile and 630 other anaerobic and aerobic organisms of intestinal origin. LFF571 possesses potent activity against C. difficile and most other Gram-positive anaerobes (MIC(90), ≤ 0.25 μg/ml), with the exception of bifidobacteria and lactobacilli...
May 2012: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/21637015/human-intestinal-microbiota-characterization-of-a-simplified-and-stable-gnotobiotic-rat-model
#15
Natalie Becker, Julia Kunath, Gunnar Loh, Michael Blaut
The study of host microbe interactions is hampered by the complexity and inter-individual variability of the human gut microbiota. Therefore, a simplified human intestinal microbiota (SIHUMI) consisting of seven bacterial species was introduced into germfree rats. Species selection was based on numerical importance and fermentative abilities in the human gut. Association of the rats with the SIHUMI (Anaerostipes caccae, Bacteroides thetaiotaomicron, Bifidobacterium longum, Blautia producta, Clostridium ramosum, Escherichia coli and Lactobacillus plantarum) resulted in increased faecal concentrations of short chain fatty acids compared to germfree animals...
January 2011: Gut Microbes
https://www.readbyqxmd.com/read/19783749/effect-of-carbohydrate-composition-in-barley-and-oat-cultivars-on-microbial-ecophysiology-and-proliferation-of-salmonella-enterica-in-an-in-vitro-model-of-the-porcine-gastrointestinal-tract
#16
Robert Pieper, Jérôme Bindelle, Brian Rossnagel, Andrew Van Kessel, Pascal Leterme
The influence of the carbohydrate (CHO) composition of cereal cultivars on microbial ecophysiology was studied using an in vitro model of the porcine gastrointestinal tract. Ten hull-less barley cultivars, six barley cultivars with hulls, six oat cultivars, and six oat groats that differed in beta-glucan, nonstarch polysaccharide (NSP), and starch contents and starch type were hydrolyzed enzymatically and incubated for 72 h with pig feces. Fermentation kinetics were modeled, and microbial compositions and short-chain fatty acid (SCFA) profiles were analyzed using terminal restriction fragment length polymorphism and gas chromatography...
November 2009: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/19736399/genetic-diversity-among-toxigenic-clostridia-isolated-from-soil-water-meat-and-associated-polluted-sites-in-south-india
#17
S Sathish, K Swaminathan
PURPOSE: To investigate the genetic diversity of toxigenic Clostridium strains isolated from soil, water, meat and its associated polluted sites of Southern India. MATERIALS AND METHODS: A total of 27 identified isolates of six different toxigenic clostridial species including C. bifermentans , C. botulinum , C. chauvoei , C. ramosum , C. tetani and C. novyi were isolated and characterized by conventional DNA restriction digestion analysis (REA) and by whole-cell and excretory protein patterns on SDS-PAGE...
October 2009: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/19712374/molecular-inventory-of-faecal-microflora-in-patients-with-crohn-s-disease
#18
Irène Mangin, Régis Bonnet, Philippe Seksik, Lionel Rigottier-Gois, Malène Sutren, Yoram Bouhnik, Christel Neut, Matthew D Collins, Jean-Frédéric Colombel, Philippe Marteau, Joël Doré
Intestinal microbial community is involved in the pathogenesis of Crohn's disease, but knowledge of its potential abnormalities has been limited by the impossibility to grow many dominant intestinal bacteria. Using sequence analysis of randomly cloned bacterial 16S ribosomal DNA, the dominant faecal species from four Crohn's disease patients and four controls were compared. Whereas marked inter-individual differences were observed in the faecal microflora of patients, three remained distantly related to controls on the basis of their operational taxonomic unit composition...
October 1, 2004: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/19240076/comparative-in-vitro-activity-of-rep3123-against-clostridium-difficile-and-other-anaerobic-intestinal-bacteria
#19
COMPARATIVE STUDY
Diane M Citron, Yumi A Warren, Kerin L Tyrrell, Vreni Merriam, Ellie J C Goldstein
OBJECTIVES: The aim of this study was to determine the anaerobic spectrum of activity of REP3123, a novel diaryldiamine that inhibits bacterial methionyl-tRNA synthetases in Gram-positive bacteria. METHODS: Fifty recent clinical isolates of Clostridium difficile from patients diagnosed with C. difficile infection and 223 other intestinal normal flora anaerobes were tested for their susceptibility to REP3123 and four or five comparator agents by the agar dilution method using supplemented Brucella agar with 5% laked sheep blood...
May 2009: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/18672296/epidemiology-of-clostridium-species-bacteremia-in-calgary-canada-2000-2006
#20
J Leal, D B Gregson, T Ross, D L Church, K B Laupland
OBJECTIVES: To define the incidence, risk factors for acquisition, and outcomes associated with clostridial bacteremia in a large Canadian health region. METHODS: Retrospective population-based surveillance for clostridial bacteremia was conducted among all residents of the Calgary Health Region (population 1.2 million) during 2000-2006. RESULTS: One hundred and thirty-eight residents had incident Clostridium species bacteremia (1.8 per 100,000/year); 45 (33%) were nosocomial, 55 (40%) were healthcare-associated community onset, and 38 (28%) were community acquired...
September 2008: Journal of Infection
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