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https://www.readbyqxmd.com/read/28806336/musical-rehabilitation-in-adult-cochlear-implant-recipients-with-a-self-administered-software
#1
Leah Smith, Lee Bartel, Samidha Joglekar, Joseph Chen
OBJECTIVE: The goal of this study was to determine if a self-administered computer-based rehabilitation program could improve music appreciation and speech understanding in adults who have a cochlear implant (CI). STUDY DESIGN: Prospective study. SETTING: Tertiary adult CI program. PATIENTS: Twenty-one postlingually deafened cochlear implant users between the ages of 27 and 79 years were recruited. INTERVENTIONS(S): A self-administered music rehabilitative software was designed to help improve the perception of musical patterns of increasing complexity, as well as pitch and timbre perception, premised on focused and divided attention...
September 2017: Otology & Neurotology
https://www.readbyqxmd.com/read/28799737/method-to-assemble-genomic-dna-fragments-or-genes-on-human-artificial-chromosome-with-regulated-kinetochore-using-a-multi-integrase-system
#2
Nicholas C O Lee, Jung-Hyun Kim, Nikolai Petrov, Hee-Sheung Lee, Hiroshi Masumoto, William C Earnshaw, Vladimir Larionov, Natalay Kouprina
The production of cells capable of carrying multiple transgenes to Mb-size genomic loci has multiple applications in biomedicine and biotechnology. In order to achieve this goal, three key steps are required: i) Cloning of large genomic segments; ii) Insertion of multiple DNA blocks at a precise location and iii) The capability to eliminate the assembled region from cells. In this study, we designed the Iterative Integration System (IIS) that utilizes recombinases Cre, ΦC31 and ΦBT1, and combined it with Human Artificial Chromosome (HAC) possessing a regulated kinetochore (alphoid(tetO)-HAC)...
August 11, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28798846/characterization-and-analysis-of-real-time-capillary-convective-pcr-toward-commercialization
#3
Xianbo Qiu, Shiyin Zhang, Lanju Mei, Di Wu, Qi Guo, Ke Li, Shengxiang Ge, Xiangzhong Ye, Ningshao Xia, Michael G Mauk
Almost all the reported capillary convective polymerase chain reaction (CCPCR) systems to date are still limited to research use stemming from unresolved issues related to repeatability, reliability, convenience, and sensitivity. To move CCPCR technology forward toward commercialization, a couple of critical strategies and innovations are discussed here. First, single- and dual-end heating strategies are analyzed and compared between each other. Especially, different solutions for dual-end heating are proposed and discussed, and the heat transfer and fluid flow inside the capillary tube with an optimized dual-end heating strategy are analyzed and modeled...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28790966/randomized-controlled-study-of-a-remote-flipped-classroom-neuro-otology-curriculum
#4
Frederick Robert Carrick, Mahera Abdulrahman, Ahmed Hankir, Maksim Zayaruzny, Kinda Najem, Palita Lungchukiet, Roger A Edwards
CONTEXT: Medical Education can be delivered in the traditional classroom or via novel technology including an online classroom. OBJECTIVE: To test the hypothesis that learning in an online classroom would result in similar outcomes as learning in the traditional classroom when using a flipped classroom pedagogy. DESIGN: Randomized controlled trial. A total of 274 subjects enrolled in a Neuro-otology training program for non-Neuro-otologists of 25 h held over a 3-day period...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28783745/an-integrated-surgical-protocol-for-adult-patients-with-hemifacial-microsomia-methods-and-outcome
#5
Kazuaki Yamaguchi, Daniel Lonic, Ellen Wen-Ching Ko, Lun-Jou Lo
BACKGROUND: Hemifacial microsomia (HFM) features hypoplasia and asymmetry in skeletal as well as soft tissue, and correction of the deformity is difficult in terms of aesthetic outcome. The purpose of this study is to examine the validity of an integrated treatment protocol for correction of this facial deformity. PATIENTS AND METHODS: A retrospective study was performed on adult HFM patients who received two-jaw orthognathic surgery combined with facial contouring procedures in the first stage, and fat injection for the residual facial deficiency in the second stage...
2017: PloS One
https://www.readbyqxmd.com/read/28781166/cryo-em-structure-of-a-pre-catalytic-human-spliceosome-primed-for-activation
#6
Karl Bertram, Dmitry E Agafonov, Olexandr Dybkov, David Haselbach, Majety N Leelaram, Cindy L Will, Henning Urlaub, Berthold Kastner, Reinhard Lührmann, Holger Stark
Little is known about the spliceosome's structure before its extensive remodeling into a catalytically active complex. Here, we report a 3D cryo-EM structure of a pre-catalytic human spliceosomal B complex. The U2 snRNP-containing head domain is connected to the B complex main body via three main bridges. U4/U6.U5 tri-snRNP proteins, which are located in the main body, undergo significant rearrangements during tri-snRNP integration into the B complex. These include formation of a partially closed Prp8 conformation that creates, together with Dim1, a 5' splice site (ss) binding pocket, displacement of Sad1, and rearrangement of Brr2 such that it contacts its U4/U6 substrate and is poised for the subsequent spliceosome activation step...
August 10, 2017: Cell
https://www.readbyqxmd.com/read/28780544/using-mixed-methods-to-assess-fidelity-of-delivery-and-its-influencing-factors-in-a-complex-self-management-intervention-for-people-with-osteoarthritis-and-low-back-pain
#7
Elaine Toomey, James Matthews, Deirdre A Hurley
OBJECTIVES AND DESIGN: Despite an increasing awareness of the importance of fidelity of delivery within complex behaviour change interventions, it is often poorly assessed. This mixed methods study aimed to establish the fidelity of delivery of a complex self-management intervention and explore the reasons for these findings using a convergent/triangulation design. SETTING: Feasibility trial of the Self-management of Osteoarthritis and Low back pain through Activity and Skills (SOLAS) intervention (ISRCTN49875385), delivered in primary care physiotherapy...
August 4, 2017: BMJ Open
https://www.readbyqxmd.com/read/28779782/future-research-directions-to-improve-fistula-maturation-and-reduce-access-failure
#8
REVIEW
Haidi Hu, Sandeep Patel, Jesse J Hanisch, Jeans M Santana, Takuya Hashimoto, Hualong Bai, Tambudzai Kudze, Trenton R Foster, Jianming Guo, Bogdan Yatsula, Janice Tsui, Alan Dardik
With the increasing prevalence of end-stage renal disease, there is a growing need for hemodialysis. Arteriovenous fistulae (AVF) are the preferred type of vascular access for hemodialysis, but maturation and failure continue to present significant barriers to successful fistula use. AVF maturation integrates outward remodeling with vessel wall thickening in response to drastic hemodynamic changes in the setting of uremia, systemic inflammation, oxidative stress, and pre-existent vascular pathology. AVF can fail due to both failure to mature adequately to support hemodialysis and development of neointimal hyperplasia that narrows the AVF lumen, typically near the fistula anastomosis...
December 2016: Seminars in Vascular Surgery
https://www.readbyqxmd.com/read/28777799/implementation-of-a-diabetes-in-pregnancy-clinical-register-in-a-complex-setting-findings-from-a-process-evaluation
#9
Renae Kirkham, Cherie Whitbread, Christine Connors, Elizabeth Moore, Jacqueline A Boyle, Richa Richa, Federica Barzi, Shu Li, Michelle Dowden, Jeremy Oats, Chrissie Inglis, Margaret Cotter, Harold D McIntyre, Marie Kirkwood, Paula Van Dokkum, Stacey Svenson, Paul Zimmet, Jonathan E Shaw, Kerin O'Dea, Alex Brown, Louise Maple-Brown
BACKGROUND: Rates of diabetes in pregnancy are disproportionately higher among Aboriginal than non-Aboriginal women in Australia. Additional challenges are posed by the context of Aboriginal health including remoteness and disadvantage. A clinical register was established in 2011 to improve care coordination, and as an epidemiological and quality assurance tool. This paper presents results from a process evaluation identifying what worked well, persisting challenges and opportunities for improvement...
2017: PloS One
https://www.readbyqxmd.com/read/28774291/health-service-changes-to-address-diabetes-in-pregnancy-in-a-complex-setting-perspectives-of-health-professionals
#10
R Kirkham, J A Boyle, C Whitbread, M Dowden, C Connors, S Corpus, L McCarthy, J Oats, H D McIntyre, E Moore, K O'Dea, A Brown, L Maple-Brown
BACKGROUND: Australian Aboriginal and Torres Strait Islander women have high rates of gestational and pre-existing type 2 diabetes in pregnancy. The Northern Territory (NT) Diabetes in Pregnancy Partnership was established to enhance systems and services to improve health outcomes. It has three arms: a clinical register, developing models of care and a longitudinal birth cohort. This study used a process evaluation to report on health professional's perceptions of models of care and related quality improvement activities since the implementation of the Partnership...
August 3, 2017: BMC Health Services Research
https://www.readbyqxmd.com/read/28764752/facilitated-interprofessional-implementation-of-a-physical-rehabilitation-guideline-for-stroke-in-inpatient-settings-process-evaluation-of-a-cluster-randomized-trial
#11
Nancy M Salbach, Sharon Wood-Dauphinee, Johanne Desrosiers, Janice J Eng, Ian D Graham, Susan B Jaglal, Nicol Korner-Bitensky, Marilyn MacKay-Lyons, Nancy E Mayo, Carol L Richards, Robert W Teasell, Merrick Zwarenstein, Mark T Bayley
BACKGROUND: The Stroke Canada Optimization of Rehabilitation by Evidence-Implementation Trial (SCORE-IT) showed that a facilitated knowledge translation (KT) approach to implementing a stroke rehabilitation guideline was more likely than passive strategies to improve functional walking capacity, but not gross manual dexterity, among patients in rehabilitation hospitals. This paper presents the results of a planned process evaluation designed to assess whether the type and number of recommended treatments implemented by stroke teams in each group would help to explain the results related to patient outcomes...
August 1, 2017: Implementation Science: IS
https://www.readbyqxmd.com/read/28750775/breakdown-of-long-range-temporal-correlations-in-brain-oscillations-during-general-anesthesia
#12
Dominik Krzemiński, Maciej Kamiński, Artur Marchewka, Michał Bola
Consciousness has been hypothesized to emerge from complex neuronal dynamics, which prevails when brain operates in a critical state. Evidence supporting this hypothesis comes mainly from studies investigating neuronal activity on a short time-scale of seconds. However, a key aspect of criticality is presence of scale-free temporal dependencies occurring across a wide range of time-scales. Indeed, robust long-range temporal correlations (LRTCs) are found in neuronal oscillations during conscious states, but it is not known how LRTCs are affected by loss of consciousness...
July 24, 2017: NeuroImage
https://www.readbyqxmd.com/read/28743884/molecular-mechanisms-of-bdp1-in-tfiiib-assembly-and-rna-polymerase-iii-transcription-initiation
#13
Jerome Gouge, Nicolas Guthertz, Kevin Kramm, Oleksandr Dergai, Guillermo Abascal-Palacios, Karishma Satia, Pascal Cousin, Nouria Hernandez, Dina Grohmann, Alessandro Vannini
Initiation of gene transcription by RNA polymerase (Pol) III requires the activity of TFIIIB, a complex formed by Brf1 (or Brf2), TBP (TATA-binding protein), and Bdp1. TFIIIB is required for recruitment of Pol III and to promote the transition from a closed to an open Pol III pre-initiation complex, a process dependent on the activity of the Bdp1 subunit. Here, we present a crystal structure of a Brf2-TBP-Bdp1 complex bound to DNA at 2.7 Å resolution, integrated with single-molecule FRET analysis and in vitro biochemical assays...
July 25, 2017: Nature Communications
https://www.readbyqxmd.com/read/28739882/zebrafish-b-cell-development-without-a-pre-b-cell-stage-revealed-by-cd79-fluorescence-reporter-transgenes
#14
Xingjun Liu, Yue-Sheng Li, Susan A Shinton, Jennifer Rhodes, Lingjuan Tang, Hui Feng, Cicely A Jette, A Thomas Look, Kyoko Hayakawa, Richard R Hardy
CD79a and CD79b proteins associate with Ig receptors as integral signaling components of the B cell Ag receptor complex. To study B cell development in zebrafish, we isolated orthologs of these genes and performed in situ hybridization, finding that their expression colocalized with IgH-μ in the kidney, which is the site of B cell development. CD79 transgenic lines were made by linking the promoter and upstream regulatory segments of CD79a and CD79b to enhanced GFP to identify B cells, as demonstrated by PCR analysis of IgH-μ expression in sorted cells...
July 24, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28736221/scaffold-curvature-mediated-novel-biomineralization-process-originates-a-continuous-soft-tissue-to-bone-interface
#15
Michael Paris, Andreas Götz, Inga Hettrich, Cécile M Bidan, John W C Dunlop, Hajar Razi, Ivo Zizak, Dietmar W Hutmacher, Peter Fratzl, Georg N Duda, Wolfgang Wagermaier, Amaia Cipitria
A myriad of shapes are found in biological tissues, often naturally evolved to fulfill a particular function. In the field of tissue engineering, substrate geometry influences cell behavior and tissue formation in-vitro, yet little is known how this translates to an in-vivo scenario. Here we investigate scaffold curvature-induced tissue growth, without additional growth factors or cells, in an ovine animal model. We show that soft tissue formation follows a curvature-driven tissue growth model. The highly organized endogenous soft matrix, potentially under mechanical strain, leads to a non-standard form of biomineralization, whereby the pre-existing organic matrix is mineralized without collagen remodeling and without an intermediate cartilage ossification phase...
July 20, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28732770/pharmacologic-modeling-of-primary-mitochondrial-respiratory-chain-dysfunction-in-zebrafish
#16
James Byrnes, Rebecca Ganetzky, Richard Lightfoot, Michael Tzeng, Eiko Nakamaru-Ogiso, Christoph Seiler, Marni J Falk
Mitochondrial respiratory chain (RC) disease is a heterogeneous and highly morbid group of energy deficiency disorders for which no proven effective therapies exist. Robust vertebrate animal models of primary RC dysfunction are needed to explore the effects of variation in RC disease subtypes, tissue-specific manifestations, and major pathogenic factors contributing to each disorder, as well as their pre-clinical response to therapeutic candidates. We have developed a series of zebrafish (Danio rerio) models that inhibit, to variable degrees, distinct aspects of RC function, and enable quantification of animal development, survival, behaviors, and organ-level treatment effects on function as well as mitochondrial biochemistry and physiology...
July 18, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/28721026/a-community-virtual-ward-model-to-support-older-persons-with-complex-health-care-and-social-care-needs
#17
C Lewis, Z Moore, F Doyle, A Martin, D Patton, L E Nugent
BACKGROUND: Globally the older population is increasing rapidly. As a result there is an increase in frail older persons living within the community, with increased risks of a hospital admission and higher mortality and morbidity rates. Due to complexity of care, health care professionals face challenges in providing effective case management and avoiding unplanned admissions to hospital. A community virtual ward (CVW) model was developed to assist health care professionals to support older persons at home during periods of illness and/or functional decline...
2017: Clinical Interventions in Aging
https://www.readbyqxmd.com/read/28710131/gene-regulatory-networks-for-the-haploid-to-diploid-transition-of-chlamydomonas-reinhardtii
#18
Sunjoo Joo, Yoshiki Nishimura, Evan Cronmiller, Ran Ha Hong, Thamali Kariyawasam, Ming Hsiu Wang, Nai Chun Shao, Saif-El-Din El Akkad, Takamasa Suzuki, Tetsuya Higashiyama, EonSeon Jin, Jae-Hyeok Lee
The sexual cycle of the unicellular Chlamydomonas reinhardtii culminates in the formation of diploid zygotes which differentiate into dormant spores that eventually undergo meiosis. Mating between gametes induces rapid cell wall shedding via the enzyme g-lysin; cell fusion is followed by heterodimerization of sex-specific homeobox transcription factors, GSM1 and GSP1, and initiation of zygote-specific gene expression. To investigate the genetic underpinnings of the zygote developmental pathway, we performed comparative transcriptome analysis of both pre- and post-fertilization samples...
July 14, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28709139/the-raman-and-sers-spectra-of-indigo-and-indigo-ag2-complex-dft-calculation-and-comparison-with-experiment
#19
Marilena Ricci, Cristiana Lofrumento, Maurizio Becucci, Emilio M Castellucci
Using time-dependent density functional theory in conjunction with B3LYP functional and LANL2DZ/6-31+g(d,p) basis sets, static and pre-resonance Raman spectra of the indigo-Ag2 complex have been calculated. Structure optimization, excitation energies and pre-resonance Raman spectra of the indigo molecule have been obtained at the same level of theory. The available experimental Raman spectra at 1064, 785 and 514nm and the SERS spectra at 785 and 514nm have been well reproduced by the calculation. Experimental SERS spectra are confronted with the calculated pre-resonance Raman spectra obtained for the indigo-Ag2 complex...
July 6, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28685140/when-a-ribosomal-protein-grows-up-the-ribosome-assembly-path-of-rps3
#20
COMMENT
Brigitte Pertschy
The biogenesis of ribosomes is a central process in all dividing cells. Eukaryotic ribosomes are composed of a large 60S and a small 40S subunit, each comprising a complex assembly of ribosomal RNA (rRNA) and ribosomal proteins (r-proteins). The synthesis of these constituents is spatially separated, with r-proteins being produced by translation in the cytoplasm, while rRNA is generated by transcription in the nucleus. Hence, the arrangement of r-proteins and rRNA into large ribonucleoprotein complexes requires dedicated mechanisms ensuring their encounter in the same compartment...
March 27, 2017: Microbial Cell
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