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Pre-integration complex

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https://www.readbyqxmd.com/read/29660771/integrated-approach-to-oral-health-in-aged-care-facilities-using-oral-health-practitioners-and-teledentistry-in-rural-queensland
#1
Anna Tynan, Lisa Deeth, Debra McKenzie, Carolyn Bourke, Shayne Stenhouse, Jacinta Pitt, Helen Linneman
PROBLEM: Residents of residential aged care facilities are at very high risk of developing complex oral diseases and dental problems. Key barriers exist in delivering oral health services to residential aged care facilities, particularly in regional and rural areas. DESIGN: A quality improvement study incorporating pre- and post chart audits and pre- and post consultation with key stakeholders, including staff and residents, expert opinion on cost estimates and field notes were used...
April 16, 2018: Australian Journal of Rural Health
https://www.readbyqxmd.com/read/29656667/haemodialysis-at-home-review-of-current-dialysis-machines
#2
Sabrina Haroon, Andrew Davenport
Only a minority of patients with chronic kidney disease treated by hemodialysis are currently treated at home. Until relatively recently, the only type of hemodialysis machine available for these patients was a slightly smaller version of the standard machines used for in-center dialysis treatments. Areas covered: There are now an alternative generation of dialysis machines specifically designed for home hemodialysis. The home dialysis patient wants a smaller machine, which is intuitive to use, easy to trouble shoot, robust and reliable, quick to set-up and put away, requiring minimal waste disposal...
April 14, 2018: Expert Review of Medical Devices
https://www.readbyqxmd.com/read/29651999/photolithographic-stereolithographic-tandem-fabrication-of-4d-smart-scaffolds-for-improved-stem-cell-cardiomyogenic-differentiation
#3
Shida Miao, Haitao Cui, Margaret Nowicki, Se-Jun Lee, Jose Almeida, Xuan Zhou, Wei Zhu, Xiaoliang Yao, Fahed Masood, Michael W Plesniak, Muhammad Mohiuddin, Lijie Grace Zhang
4D printing is a highly innovative additive manufacturing process for fabricate smart structures with the ability to transform over time. Significantly different from regular 4D printing techniques, this study focuses on creating novel 4D hierarchical micropatterns using a unique photolithographic-stereolithographic-tandem strategy (PSTS) with smart soybean oil epoxidized acrylate (SOEA) inks for effectively regulating human bone marrow mesenchymal stem cell (hMSC) cardiomyogenic behaviors. The 4D effect refers to autonomous conversion of the surficial-patterned scaffold into a pre-designed construct through an external stimulus delivered immediately after printing...
April 13, 2018: Biofabrication
https://www.readbyqxmd.com/read/29643244/sun1-regulates-hiv-1-nuclear-import-in-a-manner-dependent-on-the-interaction-between-the-viral-capsid-and-cellular-cyclophilin-a
#4
Xinlong Luo, Wei Yang, Guangxia Gao
HIV-1 can infect non-dividing cells via passing through the nuclear pore complex. The nuclear membrane imbedded protein SUN2 was recently reported to be involved in the nuclear import of HIV-1. Whether SUN1, which shares many functional similarities with SUN2, is involved in this process remained to be explored. Here, we report that overexpression of SUN1 specifically inhibited the infection of HIV-1, but not simian immunodeficiency virus (SIV) or murine leukemia virus (MLV). Overexpression of SUN1 did not affect reverse transcription, but led to reduced accumulation of the 2-LTR circular DNA and integrated viral DNA, suggesting a block in the process of nuclear import...
April 11, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29643241/truncated-cpsf6-forms-higher-order-complexes-that-bind-and-disrupt-hiv-1-capsid
#5
Jiying Ning, Zhou Zhong, Douglas K Fischer, Gemma Harris, Simon C Watkins, Zandrea Ambrose, Peijun Zhang
Cleavage and polyadenylation specificity factor 6 (CPSF6) is a human protein that binds HIV-1 capsid and mediates nuclear transport and integration targeting of HIV-1 pre-integration complexes. Truncation of the protein at its C-terminal nuclear-targeting arginine/serine-rich (RS-) domain produces a protein, CPSF6-358, that potently inhibits HIV-1 infection by targeting the capsid and inhibiting nuclear entry. To understand the molecular mechanism behind this restriction, the interaction between CPSF6-358 and HIV-1 capsid was characterized using in vitro and in vivo assays...
April 11, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29605924/unstable-protein-purification-through-the-formation-of-stable-complexes
#6
Sylvia Eiler, Nicolas Levy, Benoit Maillot, Julien Batisse, Karine Pradeau Aubreton, Oyindamola Oladosu, Marc Ruff
Purification of proteins containing disordered regions and participating in transient complexes is often challenging because of the small amounts available after purification, their heterogeneity, instability, and/or poor solubility. To circumvent these difficulties, we set up a methodology that enables the production of stable complexes in large amounts for structural and functional studies. In this chapter, we describe the methodology used to establish the best cell culture conditions and buffer compositions to optimize soluble protein production and their stabilization through protein complex formation...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29596291/interprofessional-simulations-promote-knowledge-retention-and-enhance-perceptions-of-teamwork-skills-in-a-surgical-trauma-burn-intensive-care-unit-setting
#7
Katie L George, Beth Quatrara
BACKGROUND: The current state of health care encompasses highly acute, complex patients, managed with ever-changing technology. The ability to function proficiently in critical care relies on knowledge, technical skills, and interprofessional teamwork. Integration of these factors can improve patient outcomes. Simulation provides "hands-on" practice and allows for the integration of teamwork into knowledge/skill training. However, simulation can require a significant investment of time, effort, and financial resources...
May 2018: Dimensions of Critical Care Nursing: DCCN
https://www.readbyqxmd.com/read/29589958/regulation-of-rna-polymerase-i-transcription-in-development-disease-and-aging
#8
Samim Sharifi, Holger Bierhoff
Ribosome biogenesis is a complex and highly energy-demanding process that requires the concerted action of all three nuclear RNA polymerases (Pol I-III) in eukaryotes. The three largest ribosomal RNAs (rRNAs) originate from a precursor transcript (pre-rRNA) that is encoded by multicopy genes located in the nucleolus. Transcription of these rRNA genes (rDNA) by Pol I is the key regulation step in ribosome production and is tightly controlled by an intricate network of signaling pathways and epigenetic mechanisms...
March 28, 2018: Annual Review of Biochemistry
https://www.readbyqxmd.com/read/29588650/outlier-detection-in-blast-hits
#9
Nidhi Shah, Stephen F Altschul, Mihai Pop
Background: An important task in a metagenomic analysis is the assignment of taxonomic labels to sequences in a sample. Most widely used methods for taxonomy assignment compare a sequence in the sample to a database of known sequences. Many approaches use the best BLAST hit(s) to assign the taxonomic label. However, it is known that the best BLAST hit may not always correspond to the best taxonomic match. An alternative approach involves phylogenetic methods, which take into account alignments and a model of evolution in order to more accurately define the taxonomic origin of sequences...
2018: Algorithms for Molecular Biology: AMB
https://www.readbyqxmd.com/read/29588636/enhancing-care-transitions-for-older-people-through-interprofessional-simulation-a-mixed-method-evaluation
#10
Susie Sykes, Lesley Baillie, Beth Thomas, Judy Scotter, Fiona Martin
Introduction: The educational needs of the health and social care workforce for delivering effective integrated care are important. This paper reports on the development, pilot and evaluation of an interprofessional simulation course, which aimed to support integrated care models for care transitions for older people from hospital to home. Theory and methods: The course development was informed by a literature review and a scoping exercise with the health and social care workforce...
November 13, 2017: International Journal of Integrated Care
https://www.readbyqxmd.com/read/29582349/model-reduction-in-mathematical-pharmacology-integration-reduction-and-linking-of-pbpk-and-systems-biology-models
#11
Thomas J Snowden, Piet H van der Graaf, Marcus J Tindall
In this paper we present a framework for the reduction and linking of physiologically based pharmacokinetic (PBPK) models with models of systems biology to describe the effects of drug administration across multiple scales. To address the issue of model complexity, we propose the reduction of each type of model separately prior to being linked. We highlight the use of balanced truncation in reducing the linear components of PBPK models, whilst proper lumping is shown to be efficient in reducing typically nonlinear systems biology type models...
March 26, 2018: Journal of Pharmacokinetics and Pharmacodynamics
https://www.readbyqxmd.com/read/29580223/association-between-braced-curve-behavior-by-pubertal-growth-peak-and-bracing-effectiveness-in-female-idiopathic-scoliosis-a-longitudinal-cohort-study
#12
Sai-Hu Mao, Xu Sun, Ben-Long Shi, Yong Qiu, Bang-Ping Qian, Jack C Y Cheng
BACKGROUND: Pre-pubertal idiopathic scoliosis (IS) is associated with high risk of bracing ineffectiveness. Integrated multidimensional maturity assessments are useful but complex to predict the high-risk occurrence of curve progression. This study is designed to provide a simple screening method for brace effectiveness by determining whether or not the braced curve behavior at growth spurt, being defined as variations in Cobb angle velocity (AV) at peak height velocity (PHV), can be a new factor predictive of brace outcome prescribed before PHV...
March 27, 2018: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/29576318/a-structural-model-of-the-urease-activation-complex-derived-from-ion-mobility-mass-spectrometry-and-integrative-modeling
#13
Joseph D Eschweiler, Mark A Farrugia, Sugyan M Dixit, Robert P Hausinger, Brandon T Ruotolo
The synthesis of active Klebsiella aerogenes urease via an 18-subunit enzyme apoprotein-accessory protein pre-activation complex has been well studied biochemically, but thus far this complex has remained refractory to direct structural characterization. Using ion mobility-mass spectrometry, we characterized several protein complexes between the core urease apoprotein and its accessory proteins, including the 610-kDa (UreABC)3 (UreDFG)3 complex. Using our recently developed computational modeling workflow, we generated ensembles of putative (UreABC)3 (UreDFG)3 species consistent with experimental restraints and characterized the structural ambiguity present in these models...
March 16, 2018: Structure
https://www.readbyqxmd.com/read/29574261/the-expression-of-trpv-channels-prostaglandin-e2-and-pro-inflammatory-cytokines-during-behavioural-fever-in-fish
#14
Sebastian Boltana, Nataly Sanhueza, Andrea Donoso, Andrea Aguilar, Diego Crespo, Daniela Vergara, Gabriel Arriagada, Byron Morales-Lange, Luis Mercado, Sonia Rey, Lluis Tort, Simon Mackenzie
A fever, or increased body temperature, is a symptom of inflammation, which is a complex defence reaction of the organism to pathogenic infections. After pathogens enter the body, immune cells secrete a number of agents, the functions of which stimulate the body to develop a functional immune and fever response. In mammals it is known that PGE2 is the principal mediator of fever. The extent to which PGE2 and other pro-inflammatory cytokines such as TNF-α, IL-6, or IL-1β could be involved in the induction of behavioural fever in fish remains to be clarified...
March 21, 2018: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/29570265/integrated-analysis-of-exosomal-protein-biomarkers-on-alternating-current-electrokinetic-chips-enables-rapid-detection-of-pancreatic-cancer-in-patient-blood
#15
Jean M Lewis, Ankit D Vyas, Yuqi Qiu, Karen S Messer, Rebekah White, Michael J Heller
Pancreatic ductal adenocarcinoma (PDAC) typically has non-specific symptoms, and is often found too late to treat. Because diagnosis of PDAC involves complex, invasive, and expensive procedures, screening populations at increased risk will depend on developing rapid, sensitive, specific, and cost-effective tests. Exosomes, which are nanoscale vesicles shed into blood from tumors, have come into focus as valuable entities for non-invasive liquid biopsy diagnostics. However, rapid capture and analysis of exosomes with their protein and other biomarkers have proven difficult...
March 23, 2018: ACS Nano
https://www.readbyqxmd.com/read/29559921/nitric-oxide-orchestrates-a-power-law-modulation-of-sympathetic-firing-behaviors-in-neonatal-rat-spinal-cords
#16
Chun-Kuei Su, Yi-Yin Chen, Chiu-Ming Ho
Nitric oxide (NO) is a diffusible gas and has multifarious effects on both pre- and postsynaptic events. As a consequence of complex excitatory and inhibitory integrations, NO effects on neuronal activities are heterogeneous. Using in vitro preparations of neonatal rats that retain the splanchnic sympathetic nerves and the thoracic spinal cord as an experimental model, we report here that either enhancement or attenuation of NO production in the neonatal rat spinal cords could increase, decrease, or not change the spontaneous firing behaviors recorded from splanchnic sympathetic single fibers...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29559546/microsurgical-manipulation-reveals-pre-copulatory-function-of-key-genital-sclerites
#17
Oliver T M Cocks, Paul E Eady
The copulatory organs of male insects are generally complex, species-specific arrangements of hardened sclerotized plates bound together by flexible, less sclerotized cuticle. Their extensive morphological diversification is a recurrent pattern in the evolutionary radiation of animals, yet a clear consensus as to what selection pressures drive this divergence is still to emerge. In part, this stems from the fact that the function of individual sclerites that integrate to form the aedeagus are poorly understood...
March 20, 2018: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29557665/deterministic-integration-of-quantum-dots-into-on-chip-multi-mode-interference-beamsplitters-using-in-situ-electron-beam-lithography
#18
Peter Schnauber, Johannes Schall, Samir Bounouar, Theresa Höhne, Suk-In Park, Geun-Hwan Ryu, Tobias Heindel, Sven Burger, Jin Dong Song, Sven Rodt, Stephan Reitzenstein
The development of multi-node quantum optical circuits has attracted great attention in recent years. In particular, interfacing quantum-light sources, gates and detectors on a single chip is highly desirable for the realization of large networks. In this context, fabrication techniques that enable the deterministic integration of pre-selected quantum-light emitters into nanophotonic elements play a key role when moving forward to circuits containing multiple emitters. Here, we present the deterministic integration of an InAs quantum dot into a 50/50 multi-mode interference beamsplitter via in-situ electron beam lithography...
March 20, 2018: Nano Letters
https://www.readbyqxmd.com/read/29555581/polycomb-trithorax-group-dependent-regulation-of-the-neuronal-gene-lim3-involved-in-drosophila-lifespan-control
#19
Olga Y Rybina, Yakov M Rozovsky, Ekaterina R Veselkina, Elena G Pasyukova
Molecular mechanisms governing gene expression and defining complex phenotypes are central to understanding the basics of development and aging. Here, we demonstrate that naturally occurring polymorphisms of the Lim3 regulatory region that are associated with variation in gene expression and Drosophila lifespan control are located exclusively in the Polycomb response element (PRE). We find that the Polycomb group (PcG) protein Polycomb (PC) is bound to the PRE only in embryos where Lim3 is present in both repressed and active states...
March 16, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29538042/the-evolving-genomic-landscape-in-urothelial-cancer
#20
Lara Kujtan, Arif Hussain, Janakiraman Subramanian, Ashiq Masood
PURPOSE OF REVIEW: Recent advances in next-generation sequencing have allowed for detailed molecular analysis of urothelial carcinomas, with potentially significant clinical implications for personalized treatment. Our objective in this review is to highlight studies from the past year that have furthered the understanding of urothelial cancer genomics. RECENT FINDINGS: Recent studies by The Cancer Genome Atlas consortium further characterized urothelial carcinomas via molecular subtyping, and a schema was proposed to match each subtype with potential therapeutic implications...
March 13, 2018: Current Opinion in Oncology
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