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https://www.readbyqxmd.com/read/28188886/propofol-inhibits-high-glucose-induced-pp2a-expression-in-human-umbilical-vein-endothelial-cells
#1
Qichao Wu, Yanjun Zhao, Wenming Duan, Yi Liu, Xiangyuan Chen, Minmin Zhu
Perioperative hyperglycemia is a common clinical metabolic disorder. Hyperglycemia could induce endothelial apoptosis, dysfunction and inflammation, resulting in endothelial injury. Propofol is a widely used anesthetic drug in clinical settings. Our previous studies indicated that propofol, via inhibiting high glucose-induced PP2A expression, attenuated high glucose-induced reactive oxygen species (ROS) accumulation, thus improving endothelial apoptosis, dysfunction and inflammation. However, the mechanisms by which propofol attenuated high glucose-induced PP2A expression is still obscure...
February 8, 2017: Vascular Pharmacology
https://www.readbyqxmd.com/read/28154194/autophosphorylation-based-calcium-ca2-sensitivity-priming-and-ca2-calmodulin-inhibition-of-arabidopsis-thaliana-ca2-dependent-protein-kinase-28-cpk28
#2
Kyle W Bender, R Kevin Blackburn, Jacqueline Monaghan, Paul Derbyshire, Frank Lh Menke, Cyril Zipfel, Michael B Goshe, Raymond E Zielinski, Steven C Huber
Plant calcium (Ca(2+)) dependent protein kinases (CPKs) represent the primary Ca(2+)- dependent protein kinase activities in plant systems. CPKs are composed of a dual specificity (Ser/Thr and Tyr) kinase domain tethered to a Calmodulin-like domain (CLD) via an autoinhibitory junction (J). While regulation of CPKs by Ca(2+) has been extensively studied, the contribution of autophosphorylation in controlling CPK activity is less well understood. Furthermore, whether Calmodulin (CaM) contributes to CPK regulation, as is the case for Ca(2+)/CaM-dependent protein kinases (CaMKs) outside the plant lineage, remains an open question...
January 30, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28130410/mechanisms-for-pacap-induced-prolactin-gene-expression-in-grass-carp-pituitary-cells
#3
Chengyuan Lin, Xue Jiang, Mulan He, Ling Zhao, Tao Huang, Zhaoxiang Bian, Anderson O L Wong
In mammals, pituitary adenylate cyclase-activating polypeptide (PACAP) is a pleiotropic hormone with diverse functions but its role in prolactin (PRL) regulation is highly controversial. To shed light on PRL regulation by PACAP in fish model, grass carp pituitary cells was used as a model to examine the receptor specificity and signal transduction for PACAP modulation of PRL gene expression in the carp pituitary. Using RT-PCR, PACAP-selective PAC-I receptor was detected in carp lactotrophs. In carp pituitary cells, nanomolar doses of PACAP, but not VIP, could elevate PRL secretion and protein production with concurrent rise in PRL mRNA and these stimulatory effects were blocked by PACAP antagonist but not VIP antagonist...
January 27, 2017: Journal of Endocrinology
https://www.readbyqxmd.com/read/28130256/ca2-calmodulin-dependent-protein-kinase-ii%C3%AE-and-ii%C3%AE-mediate-tgf%C3%AE-induced-transduction-of-fibronectin-and-collagen-in-human-pulmonary-fibroblasts
#4
Subhendu Mukherjee, Wei Sheng, Rui Sun, Luke J Janssen
It is now clear that in addition to activating several complex kinase pathways (Smad, MAP kinase, PI3 kinase), TGFβ also acts by elevating [Ca2+] within the cytosol of human pulmonary fibroblasts. Ca2+/calmodulin-dependent protein kinase II (CamK II) is also known to regulate gene expression in fibroblasts. In this study, we examined the interactions between calcium signaling, activation of CamK and other kinases, and extracellular matrix (ECM) gene expression. Human pulmonary fibroblasts were cultured and stimulated with artificially-generated Ca2+-pulses in the absence of TGFβ, or with TGFβ (1 nM) or vehicle in the presence of various blockers of Ca2+ signaling...
January 27, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28031098/mechanical-strain-regulates-osteoblast-proliferation-through-ca-2-camk-creb-signal-pathway
#5
Yong Guo, Qi Lv, Xian-Qiong Zou, Zhi-Xiong Yan, Yu-Xian Yan
Objective To investigate the effects of mechanical strain on Ca(2+)-calmodulin dependent kinase (CaMK)-cAMP response element binding protein (CREB) signal pathway and proliferation of osteoblasts.Methods Using a four-point bending device, MC3T3-E1 cells were exposed to mechanical tensile strains of 2500 µs and 5000 µs at 0.5 Hz respectively. The intracellular free Ca(2+) ([Ca(2+)]i) concentration and calmodulin activity were assayed by fluorospectrophotometry, CaMK II β, CREB, and phosphorylated (activated) CREB (p-CREB) were assessed by Western blot, and cells proliferation was assayed with MTT...
June 20, 2016: Chinese Medical Sciences Journal, Chung-kuo i Hsüeh K'o Hsüeh Tsa Chih
https://www.readbyqxmd.com/read/28017803/ca-2-dependent-surface-trafficking-of-norepinephrine-transporters-depends-on-threonine-30-and-ca-2-calmodulin-kinases
#6
Uhna Sung, Francesca Binda, Valentina Savchenko, William A Owens, Lynette C Daws
The antidepressant-sensitive norepinephrine (NE) transporter (NET) inactivates NE released during central and peripheral neuronal activity by transport into presynaptic cells. Altered NE clearance due to dysfunction of NET has been associated with the development of mental illness and cardiovascular diseases. NET activity in vivo is influenced by stress, neuronal activity, hormones and drugs. We investigated the mechanisms of Ca(2+) regulation of NET and found that Ca(2+) influenced both Vmax and Km for NE transport into cortical synaptosomes...
December 23, 2016: Journal of Chemical Neuroanatomy
https://www.readbyqxmd.com/read/28011257/cellular-subtype-expression-and-activation-of-camkii-regulate-the-fate-of-atherosclerotic-plaque
#7
Angela Serena Maione, Ersilia Cipolletta, Daniela Sorriento, Francesco Borriello, Maria Soprano, Maria Rosaria Rusciano, Vittoria D'Esposito, Abdul Karim Markabaoui, Giovanni Domenico De Palma, Giovanni Martino, Lucio Maresca, Giuseppe Nobile, Pietro Campiglia, Pietro Formisano, Michele Ciccarelli, Gianni Marone, Bruno Trimarco, Guido Iaccarino, Maddalena Illario
BACKGROUND AND AIMS: Atherosclerosis is a degenerative process of the arterial wall implicating activation of macrophages and proliferation of vascular smooth muscle cells. Calcium-calmodulin dependent kinase type II (CaMKII) in vascular smooth muscle cells (VSMCs) regulates proliferation, while in macrophages, this kinase governs diapedesis, infiltration and release of extracellular matrix enzymes. We aimed at understanding the possible role of CaMKII in atherosclerosis plaques to regulate plaque evolution towards stability or instability...
January 2017: Atherosclerosis
https://www.readbyqxmd.com/read/27986626/propofol-attenuates-pancreatic-cancer-malignant-potential-via-inhibition-of-nmda-receptor
#8
Xiangyuan Chen, Qichao Wu, Li You, Sisi Chen, Minmin Zhu, Changhong Miao
Propofol is a commonly used intravenous anesthetic, and could attenuate cancer cells malignant potential via inhibiting hypoxia-inducible factor-1α (HIF-1α) expression. However, the mechanism is still inclusive. In the present study, we mainly focus on the mechanism by which propofol down-regulated HIF-1α expression and malignant potential in pancreatic cancer cells. Human pancreatic cancer cells (Miapaca-2 and Panc-1) in vitro and murine pancreatic cancer cell (Panc02) in vivo were used to assess the effect of propofol on vascular endothelial growth factor (VEGF) expression and migration of pancreatic cancer cells...
January 15, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/27888323/the-calmodulin-gene-in-neurospora-crassa-is-required-for-normal-vegetative-growth-ultraviolet-survival-and-sexual-development
#9
Vijya Laxmi, Ranjan Tamuli
We isolated a Neurospora crassa mutant of the calmodulin (cmd) gene using repeat-induced point mutation and studied its phenotypes. The cmd (RIP) mutant showed a defect in growth, reduced aerial hyphae, decreased carotenoid accumulation, a severe reduction in viability upon ultraviolet (UV) irradiation, and a fertility defect. Moreover, meiotic silencing of the cmd gene resulted in a barren phenotype. In addition, we also performed site-directed mutational analysis of the calcium/calmodulin-dependent kinase-2 (Ca(2+)/CaMK-2), a target of the CaM protein encoded by the cmd gene...
November 25, 2016: Archives of Microbiology
https://www.readbyqxmd.com/read/27883090/sphingosine-1-phosphate-lyase-ablation-disrupts-presynaptic-architecture-and-function-via-an-ubiquitin-proteasome-mediated-mechanism
#10
Daniel N Mitroi, André U Deutschmann, Maren Raucamp, Indulekha Karunakaran, Konstantine Glebov, Michael Hans, Jochen Walter, Julie Saba, Markus Gräler, Dan Ehninger, Elena Sopova, Oleg Shupliakov, Dieter Swandulla, Gerhild van Echten-Deckert
The bioactive lipid sphingosine 1-phosphate (S1P) is a degradation product of sphingolipids that are particularly abundant in neurons. We have shown previously that neuronal S1P accumulation is toxic leading to ER-stress and an increase in intracellular calcium. To clarify the neuronal function of S1P, we generated brain-specific knockout mouse models in which S1P-lyase (SPL), the enzyme responsible for irreversible S1P cleavage was inactivated. Constitutive ablation of SPL in the brain (SPL(fl/fl/Nes)) but not postnatal neuronal forebrain-restricted SPL deletion (SPL(fl/fl/CaMK)) caused marked accumulation of S1P...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27878554/the-involvement-of-the-decrease-of-astrocytic-wnt5a-in-the-cognitive-decline-in-minimal-hepatic-encephalopathy
#11
Saidan Ding, Zhu Xu, Jianjing Yang, Leping Liu, Xueli Huang, Xuebao Wang, Qichuan Zhuge
Wnt signaling plays a key role in neuroprotection and synaptic plasticity. We speculate that the impairment of Wnt signaling may mediate astrocytic neurotrophins (NTs) production and the impairment of Wnt signaling to astrocytic NTs production contributes to the pathogenesis of minimal hepatic encephalopathy (MHE). Here, we found that induction of astrocytic NTs synthesis was by Wnt5a via the calcium/calmodulin-sensitive protein kinase II (CaMK II)-cAMP-response element-binding protein (CREB) pathway in PCAs...
November 22, 2016: Molecular Neurobiology
https://www.readbyqxmd.com/read/27863837/the-effects-of-ropivacaine-hydrochloride-on-the-expression-of-camk-ii-mrna-in-the-dorsal-root-ganglion-neurons
#12
Xianjie Wen, Xiaohong Lai, Xiaohong Li, Tao Zhang, Hua Liang
In this study, we identified the subtype of Calcium/calmodulin-dependent protein kinase II (CaMK II) mRNA in dorsal root ganglion neurons and observed the effects of ropivacaine hydrochloride in different concentration and different exposure time on the mRNA expression. Dorsal root ganglion neurons were isolated from the SD rats and cultured in vitro. The mRNA of the CaMK II subtype in dorsal root ganglion neurons were detected by real-time PCR. As well as, the dorsal root ganglion neurons were treated with ropivacaine hydrochloride in different concentration (1mM,2mM, 3mM and 4mM) for the same exposure time of 4h, or different exposure time (0h,2h,3h,4h and 6h) at the same concentration(3mM)...
December 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27823926/cisplatin-induced-neuropathic-pain-is-mediated-by-upregulation-of-n-type-voltage-gated-calcium-channels-in-dorsal-root-ganglion-neurons
#13
Markus Leo, Linda-Isabell Schmitt, Martin Erkel, Margarita Melnikova, Jürgen Thomale, Tim Hagenacker
Cisplatin is important in the treatment of various types of cancer. Although it is highly effective, it also has severe side effects, with neurotoxicity in dorsal root ganglion (DRG) neurons being one of the most common. The key mechanisms of neurotoxicity are still controversially discussed; however, disturbances of the calcium homeostasis in DRG neurons have been suggested to mediate cisplatin neurotoxicity. By using the whole-cell patch-clamp technique, immunostaining and behavioral experiments with Sprague-Dawley rats, we examined the influence of short- and long-term exposure to cisplatin on voltage-gated calcium channel (VGCC) currents (ICa(V)) in small DRG neurons...
February 2017: Experimental Neurology
https://www.readbyqxmd.com/read/27806760/-the-crosstalk-between-canonical-and-noncanonical-wnt-signaling-pathway-in-osteoblast-differentiation-of-periodontal-ligament-stem-cells-in-inflammatory-microenvironments
#14
N Liu, H G Shi, W Zhang, B Gu
Objective: To investigate the crosstalk between canonical Wnt/β-catenin and noncanonical Wnt/Ca(2+) pathway in osteoblast differentiation process of periodontal ligament stem cell (PDLSC) in inflammatory microenvironments. Methods: PDLSCs were obtained from human healthy individuals(H-PDLSC) and patients with periodontitis(P-PDLSC). The H/P-PDLSCs were transfected with β-catenin siRNA. Cell morphology was observed under fluorescent microscope and transfection efficiency was easured by Western blotting after transfection of PDLSC...
November 9, 2016: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
https://www.readbyqxmd.com/read/27796283/the-pseudokinase-camkv-is-required-for-the-activity-dependent-maintenance-of-dendritic-spines
#15
Zhuoyi Liang, Yi Zhan, Yang Shen, Catherine C L Wong, John R Yates, Florian Plattner, Kwok-On Lai, Nancy Y Ip
Dendritic spine stabilization depends on afferent synaptic input and requires changes in actin cytoskeleton dynamics and protein synthesis. However, the underlying molecular mechanism remains unclear. Here we report the identification of 'calmodulin kinase-like vesicle-associated' (CaMKv), a pseudokinase of the CaMK family with unknown function, as a synaptic protein crucial for dendritic spine maintenance. CaMKv mRNA localizes at dendrites, and its protein synthesis is regulated by neuronal activity. CaMKv function is inhibited upon phosphorylation by cyclin-dependent kinase 5 (Cdk5) at Thr345...
October 31, 2016: Nature Communications
https://www.readbyqxmd.com/read/27734911/novelty-during-a-late-postacquisition-time-window-attenuates-the-persistence-of-fear-memory
#16
Cynthia Katche, Micol Tomaiuolo, Guido Dorman, Jorge H Medina, Haydee Viola
Learning to avoid threats in the environment is highly adaptive. However, sometimes a dysregulation of fear memories processing may underlie fear-related disorders. Despite recent advances, a major question of how to effectively attenuate persistent fear memories in a safe manner remains unresolved. Here we show experiments employing a behavioural tool to target a specific time window after training to limit the persistence of a fear memory in rats. We observed that exposure to a novel environment 11 h after an inhibitory avoidance (IA) training that induces a long-lasting memory, attenuates the durability of IA memory but not its formation...
October 13, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27708635/the-kinome-of-edible-and-medicinal-fungus-wolfiporia-cocos
#17
Wei Wei, Shaohua Shu, Wenjun Zhu, Ying Xiong, Fang Peng
Wolfiporia cocos is an edible and medicinal fungus that grows in association with pine trees, and its dried sclerotium, known as Fuling in China, has been used as a traditional medicine in East Asian countries for centuries. Nearly 10% of the traditional Chinese medicinal preparations contain W. cocos. Currently, the commercial production of Fuling is limited because of the lack of pine-based substrate and paucity of knowledge about the sclerotial development of the fungus. Since protein kinase (PKs) play significant roles in the regulation of growth, development, reproduction, and environmental responses in filamentous fungi, the kinome of W...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27667720/g9a-inhibits-mef2c-activity-to-control-sarcomere-assembly
#18
Jin Rong Ow, Monica Palanichamy Kala, Vinay Kumar Rao, Min Hee Choi, Narendra Bharathy, Reshma Taneja
In this study, we demonstrate that the lysine methyltransferase G9a inhibits sarcomere organization through regulation of the MEF2C-HDAC5 regulatory axis. Sarcomeres are essential for muscle contractile function. Presently, skeletal muscle disease and dysfunction at the sarcomere level has been associated with mutations of sarcomere proteins. This study provides evidence that G9a represses expression of several sarcomere genes and its over-expression disrupts sarcomere integrity of skeletal muscle cells. G9a inhibits MEF2C transcriptional activity that is essential for expression of sarcomere genes...
September 26, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27616426/membrane-receptor-cross-talk-in-gonadotropin-igf-i-and-insulin-mediated-steroidogenesis-in-fish-ovary-an-overview
#19
Dilip Mukherjee, Suravi Majumder, Sujata Roy Moulik, Puja Pal, Shreyasi Gupta, Payel Guha, Dhynendra Kumar
Gonadal steroidogenesis is critical for survival and reproduction of all animals. The pathways that regulate gonadal steroidogenesis are therefore conserved among animals from the steroidogenic enzymes to the intracellular signaling molecules and G protein-coupled receptors (GPCRs) that mediate the activity of these enzymes. Regulation of fish ovarian steroidogenesis in vitro by gonadotropin (GtH) and GPCRs revealed interaction between adenylate cyclase and calcium/calmodulin-dependent protein kinases (CaMKs) and also MAP kinase pathway...
January 1, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/27595734/vasostatin-1-stops-structural-remodeling-and-improves-calcium-handling-via-the-enos-no-pkg-pathway-in-rat-hearts-subjected-to-chronic-%C3%AE-adrenergic-receptor-activation
#20
Dandan Wang, Yingguang Shan, Yan Huang, Yanhong Tang, Yuting Chen, Ran Li, Jing Yang, Congxin Huang
PURPOSE: Chronically elevated catecholamine levels activate cardiac β-adrenergic receptors, which play a vital role in the pathogenesis of heart failure. Evidence suggests that vasostatin-1 (VS-1) exerts anti-adrenergic effects on isolated and perfused hearts in vitro. Whether VS-1 ameliorates hypertrophy/remodeling by inducing the chronic activation of β-adrenergic receptors is unknown. The present study aims to test the efficacy of using VS-1 to treat the advanced hypertrophy/remodeling that result from chronic β-adrenergic receptor activation and to determine the cellular and molecular mechanisms that underlie this response...
October 2016: Cardiovascular Drugs and Therapy
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