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https://www.readbyqxmd.com/read/28732154/ironing-out-genes-in-the-environment-an-experimental-study-of-the-dna-goethite-interface
#1
Michael P Schmidt, Carmen Enid Martinez
DNA fate in soil plays an important role in the cycling of genetic information in the environment. Adsorption onto mineral surfaces has great impact on this function. This study probes the kinetics, equilibrium behavior and bonding mechanisms associated with adsorption of DNA onto goethite, a common soil mineral. Surface sensitive ATR-FTIR and XPS approaches are applied to directly characterize the DNA-goethite interface. Adsorption kinetics follow a pseudo-first order model, suggesting adsorption rate is surface limited...
July 21, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28731571/model-based-investigation-on-the-mass-transfer-and-adsorption-mechanisms-of-mono-pegylated-lysozyme-in-ion-exchange-chromatography
#2
Josefine Morgenstern, Gang Wang, Pascal Baumann, Jürgen Hubbuch
Recent studies highlighted the potential of PEGylated proteins to improve stabilities and pharmacokinetics of protein drugs. Ion-exchange chromatography (IEX) is among the most frequently used purification methods for PEGylated proteins. However, the underlying physical mechanisms allowing for a separation of different PEGamers (proteins with a varying number of attached PEG molecules) are not yet fully understood. In this work, mechanistic chromatography modeling was applied to gain a deeper understanding of the mass transfer and adsorption/desorption mechanisms of mono-PEGylated proteins in IEX...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28730959/chemometric-analysis-of-inter-and-intra-molecular-diversification-factors-by-a-training-simplex-approach-a-review-and-research-on-astragalus-saponins
#3
Abir Sarraj-Laabidi, Habib Messai, Asma Hammami-Semmar Hammami-Semmar, Nabil Semmar
Metabolisms represent highly organized systems characterized by strong regulations obeying to mass conservation principle. This makes the whole chemical resource to be competitively shared at both intra-and inter-molecular scales. Whole resource sharing can be statistically associated to a constant sum-unit constraint which represents the basis of simplex mixture rule. In this work, a new simplex-based simulation approach was developed to learn scaffold information on metabolic processes controlling molecular diversity from a wide set of observed chemical structures...
July 19, 2017: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/28730712/slow-conduction-through-an-arc-of-block-a-basis-for-arrhythmia-formation-post-myocardial-infarction
#4
Eugene Patterson, Benjamin J Scherlag, Edward J Berbari, Ralph Lazzara
INTRODUCTION: The electrophysiologic basis for characteristic rate-dependent, constant-late-coupled (390+54 msec) premature ventricular beats (PVBs) present 4-5 days following coronary artery occlusion were examined in 108 anesthetized dogs. METHODS AND RESULTS: Fractionated/double potentials were observed in injured zone bipolar and composite electrograms at prolonged sinus cycle lengths (1296 ± 396 msec). At shorter cycle lengths, conduction of the delayed potential decremented, separating from the initial electrogram by a progressively prolonged isoelectric interval...
July 21, 2017: Journal of Cardiovascular Electrophysiology
https://www.readbyqxmd.com/read/28730200/how-side-chains-affect-conformation-and-electrical-properties-of-poly-acrylic-acid-in-solution
#5
Xinlu Zhou, Kongshuang Zhao
To better understand the effect of side chains on the chain conformation and electrical properties of polyelectrolytes, dielectric measurements were carried out on solutions of poly(acrylic acid) (PAA), poly(acrylic acid)-graft-dodecyl (PAA-g-dodecyl), and poly(acrylic acid)-graft-poly(ethylene oxide) (PAA-g-PEO) over a wide concentration range. Double dielectric relaxations with counterion distribution were observed for these polymers and a refined double-layer polarization model was proposed to analyze these, by which valuable information about conformations and interfacial electrokinetic properties was obtained...
July 21, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28729870/plastid-nucleus-distance-alters-the-behavior-of-stromules
#6
Jessica L Erickson, Matthias Kantek, Martin H Schattat
Plastids send "retrograde" signals to the nucleus to deliver information regarding their physiological status. One open question concerning this signal transfer is how the signal bridges the cytoplasm. Based on individual reports of plastid derived tubular membrane extensions connecting to nuclei, these so-called stromules have been suggested to function as communication routes between plastids and nuclei in response to biotic stress. However, based on the data currently available it is unclear whether interactions between stromules and nuclei are truly intentional or observed as a result of an inflated stromule frequency throughout the cell, and are thus a random event...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28729556/observation-of-aggregation-triggered-by-resonance-energy-transfer-ret-induced-intermolecular-pairing-force
#7
Xiaoyong Pan, Weizhi Wang, Lin Ke, Nan Zhang
In this report, we showed the existence of RET induced intermolecular pairing force by comparing their fluorescence behaviors under room illumination vs standing in dark area for either PFluAnt solution or PFluAnt&PFOBT mixture. Their prominent emission attenuation under room illumination brought out the critical role of photo, i.e. RET induced intermolecular pairing force in induction of polymer aggregation. Constant UV-Vis absorption and fluorescence spectra in terms of both peak shapes and maximum wavelengths implied no chemical decomposition was involved...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28727889/clinical-assessment-of-uterine-contractions
#8
REVIEW
Wayne R Cohen
The assessment of uterine contractions is important in clinical decision-making, but the precise role for appraising contractions remains controversial. Four clinical approaches to assessing contractions are available: manual palpation; intrauterine pressure determination; external tocodynamometry; and electrohysterography. Palpation is inexpensive and harmless but requires the constant bedside presence of a trained observer. Intrauterine pressure measurement is considered the most sensitive and specific technique, and has become the standard by which other methods are judged; however, its quantitative measurements are not always precise or reproducible...
July 20, 2017: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/28727050/net-energy-of-hemp-hulls-and-processed-hemp-hull-products-fed-to-growing-pigs-and-the-comparison-of-net-energy-determined-via-indirect-calorimetry-and-calculated-from-prediction-equations
#9
J W Kim, C M Nyachoti
An experiment was conducted to determine the NE of hemp hulls (HH), extruded HH (EHH), and a blended product of HH with pea (HHP) fed to growing pigs using indirect calorimetry (IC) and to determine effects of dietary fiber on heat production (HP) and fasting HP (FHP). Twenty-four growing barrows with an average initial BW of 22.9 ± 1.75 kg were individually housed in adjustable metabolism crates. Pigs were randomly allotted to 1 of 4 dietary treatments with 6 replicates per treatment. A corn-soybean meal basal diet was prepared...
June 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28726953/4-4-di-tert-butyl-6-1h-tetrazol-5-yl-2-2-bipyridine-modification-of-a-highly-selective-n-donor-ligand-for-the-separation-of-trivalent-actinides-from-lanthanides
#10
Martin M Maiwald, Anna T Wagner, Jochen Kratsch, Andrej Skerencak-Frech, Michael Trumm, Andreas Geist, Peter W Roesky, Petra J Panak
In the present work, the complexation and extraction behaviour of 4,4'di-tert-butyl-6-(1H-tetrazol-5-yl)-2,2'-bipyridine (HN4(t)bubipy) towards trivalent actinides (An(iii)) and lanthanides (Ln(iii)) is studied by spectroscopic methods, liquid-liquid extraction, and quantum chemical calculations. The ligand synthesis of HN4(t)bubipy as well as its application in coordination chemistry of the 4f elements is described. Reaction of HN4(t)bubipy with [Ln(NO3)3·6H2O] (Ln = Sm, Eu) results in [H2N4(t)bubipy](+)[Ln(N4(t)bubipy)(NO3)3(H2O)](-)...
July 20, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28726875/time-resolved-spectroscopic-analysis-of-the-light-energy-harvesting-mechanism-in-carbazole-dendrimers-with-a-blue-phosphorescent-ir-complex-core
#11
Yang-Jin Cho, So-Yoen Kim, Mi Rang Son, Ho-Jin Son, Dae Won Cho, Sang Ook Kang
In order to investigate the light-energy harvesting mechanism, a series of dendrimers with a heteroleptic iridium(iii) complex core, [Ir(dmb)2(pic-Czn)] (Gn: n = 1, 2, and 3), with 2,6-difluoro-3-(4-methylpyridin-2-yl)benzonitrile (dmb) as the cyclometallating ligand and 3-hydroxypicolinate (pic) as the ancillary ligand, connected to carbazole-based dendrons (Czn: n = 2, 4, and 8) was synthesized. The Ir centred complex [Ir(dmb)2(pic-OCH3), G0] shows a blue emission at <500 nm, which is assigned to metal-to-ligand charge-transfer ((3)MLCT) phosphorescence...
July 20, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28726699/detoxification-of-photo-catalytically-treated-2-chlorophenol-optimization-through-response-surface-methodology
#12
Muhammad Z Ahamd, S Ehtisham-Ul-Haque, Numrah Nisar, Khizar Qureshi, Abdul Ghaffar, Mazhar Abbas, Jan Nisar, Munawar Iqbal
The present study was conducted to degrade and detoxify 2-chlorophenol (2-CP) under UV irradiation in the presence of titanium dioxide (TiO2) and hydrogen peroxide (H2O2). The treatment efficiency was evaluated on the basis of degradation and cytotoxicity reduction as well as biochemical oxygen demand (BOD), chemical oxygen demand (COD) and total organic carbon (TOC) removal. The process variables such as TiO2, pH, UV irradiation time and H2O2 were optimized. Central composite design in combination with response surface methodology was employed to optimize the process variables...
July 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28726139/assessing-trends-in-laparoscopic-colostomy-reversal-and-evaluating-outcomes-when-compared-to-open-procedures
#13
Kevin Y Pei, Kimberly A Davis, Yawei Zhang
BACKGROUND: Laparoscopic colostomy reversal has emerged as a viable option for Hartmann's reversal but the trends in national adoption and postoperative complications are unknown. This study evaluates the practice trends for laparoscopic colostomy and compares complications, length of stay, and operative times between laparoscopic and open colostomy reversal. METHODS: All patients who had open or laparoscopic colostomy reversal surgery (current procedure codes: 44227 and 44626) between 2005 and 2014 were identified from the American College of Surgeons National Surgical Quality Improvement Program...
July 19, 2017: Surgical Endoscopy
https://www.readbyqxmd.com/read/28724866/effect-of-reduced-tube-voltage-on-diagnostic-accuracy-of-ct-colonography
#14
Yoshihiro Futamata, Tomoaki Koide, Riku Ihara
The normal tube voltage in computed tomography colonography (CTC) is 120 kV. Some reports indicate that the use of a low tube voltage (lower than 120 kV) technique plays a significant role in reduction of radiation dose. However, to determine whether a lower tube voltage can reduce radiation dose without compromising diagnostic accuracy, an evaluation of images that are obtained while maintaining the volume CT dose index (CTDIvol) is required. This study investigated the effect of reduced tube voltage in CTC, without modifying radiation dose (i...
2017: Nihon Hoshasen Gijutsu Gakkai Zasshi
https://www.readbyqxmd.com/read/28724647/physiological-responses-to-fluctuating-temperatures-are-characterized-by-distinct-transcriptional-profiles-in-a-solitary-bee
#15
Alex S Torson, George D Yocum, Joseph P Rinehart, Sean A Nash, Kally M Kvidera, Julia H Bowsher
Exposure to stressful low temperatures during development can result in the accumulation of deleterious physiological effects called chill injury. Metabolic imbalances, disruptions in ion homeostasis, and oxidative stress contribute to the increased mortality of chill-injured insects. Interestingly, survival can be significantly increased when chill susceptible insects are exposed to a daily warm-temperature pulse during chilling. We hypothesize that warm pulses allow for the repair of damage associated with chill-injury...
July 19, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28723976/benefits-of-fading-in-perceptual-learning-are-driven-by-more-than-dimensional-attention
#16
Matthew G Wisniewski, Milen L Radell, Barbara A Church, Eduardo Mercado
Individuals learn to classify percepts effectively when the task is initially easy and then gradually increases in difficulty. Some suggest that this is because easy-to-discriminate events help learners focus attention on discrimination-relevant dimensions. Here, we tested whether such attentional-spotlighting accounts are sufficient to explain easy-to-hard effects in auditory perceptual learning. In two experiments, participants were trained to discriminate periodic, frequency-modulated (FM) tones in two separate frequency ranges (300-600 Hz or 3000-6000 Hz)...
2017: PloS One
https://www.readbyqxmd.com/read/28723974/alginate-hydrogels-allow-for-bioactive-and-sustained-release-of-vegf-c-and-vegf-d-for-lymphangiogenic-therapeutic-applications
#17
Kevin T Campbell, Dustin J Hadley, David L Kukis, Eduardo A Silva
Lymphatic dysfunction is associated with the progression of many cardiovascular disorders due to their role in maintaining tissue fluid homeostasis. Promoting new lymphatic vessels (lymphangiogenesis) is a promising strategy to reverse these cardiovascular disorders via restoring lymphatic function. Vascular endothelial growth factor (VEGF) members VEGF-C and VEGF-D are both potent candidates for stimulating lymphangiogenesis, though maintaining spatial and temporal control of these factors represents a challenge to developing efficient therapeutic lymphangiogenic applications...
2017: PloS One
https://www.readbyqxmd.com/read/28723678/carrier-transport-dynamics-in-mn-doped-cdse-quantum-dot-sensitized-solar-cells
#18
Uma Poudyal, Franscis Scott Maloney, Keshab Raj Sapkota, Wenyong Wang
In this work quantum dot sensitized solar cells (QDSSCs) were fabricated with CdSe and Mn-doped CdSe quantum dots (QDs) using the SILAR method. QDSSCs based on Mn-doped CdSe QDs exhibited improved incident photon-to-electron conversion efficiency. Carrier transport dynamics in the QDSSCs were studied using the intensity modulated photocurrent/photovoltage spectroscopy technique, from which transport and recombination time constants could be derived. Compared to CdSe QDSSCs, Mn-CdSe QDSSCs exhibited shorter transport time constant, longer recombination time constant, longer diffusion length, and higher charge collection efficiency...
July 19, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28723155/inhibiting-the-fibrillation-of-serum-albumin-proteins-in-presence-of-surface-active-ionic-liquids-sails-at-low-ph-a-spectroscopic-and-microscopic-study
#19
Sangita Kundu, Chiranjib Banerjee, Nilmoni Sarkar
One of the key necessary steps to prevent human neurological disorders is the efficient disruption of protein aggregation or amyloid fibril. In this article, we have explored the effect of three amphiphilic surface active ionic liquids (SAILs) namely 1-methyl-3-octylimidazolium chloride ([C8mim]Cl), 1-dodecyl-3-methyllimidazolium chloride ([C12mim]Cl) and 1-hexadecyl-3-methyllimidazolium chloride ([C16mim]Cl) having concentration of 5.8 mM, 0.29 mM and 0.08 mM respectively on bovine serum albumin (BSA) and human serum albumin (HSA) fibril...
July 19, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28723146/generalized-noise-study-of-solid-state-nanopores-at-low-frequencies
#20
Chenyu Wen, Shuangshuang Zeng, Kai Arstila, Timo Sajavaara, Yu Zhu, Zhen Zhang, Shi-Li Zhang
Nanopore technology has been extensively investigated for analysis of biomolecules, and a success story in this field concerns DNA sequencing using a nanopore chip featuring an array of hundreds of biological nanopores (BioNs). Solid-state nanopores (SSNs) have been explored to attain longer lifetime and higher integration density than what BioNs can offer, but SSNs are generally considered to generate higher noise whose origin remains to be confirmed. Here, we systematically study low-frequency (including thermal and flicker) noise characteristics of SSNs measuring 7 to 200 nm in diameter drilled through a 20-nm-thick SiNx membrane by focused ion milling...
February 24, 2017: ACS Sensors
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