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https://www.readbyqxmd.com/read/28927195/protein-sgpr-of-pseudomonas-putida-strain-ak5-is-a-lysr-type-regulator-of-salicylate-degradation-through-gentisate
#1
Irina Yu Filatova, Alexei S Kazakov, Evgeny N Muzafarov, Marina V Zakharova
Pseudomonas putida strain AK5 was the first characterized natural strain containing the 'classical' nah1 operon and nahR gene along with genes whose products are responsible for the less explored pathway of salicylate degradation through gentisate (the sgp operon). The sgp operon was found to be preceded by the divergently directed sgpR gene. The amino acid sequence of the sgpR product qualifies it as a LysR-type transcriptional regulator (LTTR) and suggests its potential function as an sgp operon transcriptional regulator...
July 3, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28927161/needlestick-and-sharp-injuries-among-registered-nurses-a-case-control-study
#2
Gabriele d'Ettorre
Objectives: The prevention of needlestick and sharp injuries (NSSIs) among registered nurses (RNs) employed in hospital wards represents an important issue. Accidental contact with blood, consequent to NSSIs, is the most frequent form of exposure to blood-borne pathogens that can be transmitted by accidental exposure. Aims: This study investigates the relationship occurring between consecutive workdays, night-shifts, cumulative hours, forward-rotating shift schedules (morning-afternoon-night), and occupational NSSIs frequency...
June 1, 2017: Annals of Work Exposures and Health
https://www.readbyqxmd.com/read/28927008/can-more-nanoparticles-induce-larger-viscosities-of-nanoparticle-enhanced-wormlike-micellar-system-newms
#3
Mingwei Zhao, Yue Zhang, Chenwei Zou, Caili Dai, Mingwei Gao, Yuyang Li, Wenjiao Lv, Jianfeng Jiang, Yining Wu
There have been many reports about the thickening ability of nanoparticles on the wormlike micelles in the recent years. Through the addition of nanoparticles, the viscosity of wormlike micelles can be increased. There still exists a doubt: can viscosity be increased further by adding more nanoparticles? To answer this issue, in this work, the effects of silica nanoparticles and temperature on the nanoparticles-enhanced wormlike micellar system (NEWMS) were studied. The typical wormlike micelles (wormlike micelles) are prepared by 50 mM cetyltrimethyl ammonium bromide (CTAB) and 60 mM sodium salicylate (NaSal)...
September 18, 2017: Materials
https://www.readbyqxmd.com/read/28926847/probing-the-stochastic-property-of-endoreduplication-in-cell-size-determination-of-arabidopsis-thaliana-leaf-epidermal-tissue
#4
Kensuke Kawade, Hirokazu Tsukaya
Cell size distribution is highly reproducible, whereas the size of individual cells often varies greatly within a tissue. This is obvious in a population of Arabidopsis thaliana leaf epidermal cells, which ranged from 1,000 to 10,000 μm2 in size. Endoreduplication is a specialized cell cycle in which nuclear genome size (ploidy) is doubled in the absence of cell division. Although epidermal cells require endoreduplication to enhance cellular expansion, the issue of whether this mechanism is sufficient for explaining cell size distribution remains unclear due to a lack of quantitative understanding linking the occurrence of endoreduplication with cell size diversity...
2017: PloS One
https://www.readbyqxmd.com/read/28926778/korean-immigrants-don-t-buy-health-insurance-the-influences-of-culture-on-self-employed-korean-immigrants-focusing-on-structure-and-functions-of-social-networks
#5
Hyunsung Oh, Chung Hyeon Jeong
Culture has been pinpointed as a culprit of disparities in health insurance coverage between Korean immigrants and other ethnic groups. This study explored specific mechanisms by which culture influences a decision to buy health insurance among self-employed Korean immigrants living in ethnic enclaves by focusing on the structure and functions of social networks. Between March and June 2015, we recruited 24 Korean immigrant adults (aged 18 or older) who identified as self-employed and being uninsured for substantial periods before 2014 in Southern California...
September 12, 2017: Social Science & Medicine
https://www.readbyqxmd.com/read/28926716/broadband-acoustic-resonance-dissolution-spectroscopy-bards-a-novel-approach-to-investigate-the-wettability-of-pharmaceutical-powder-blends
#6
Raghu V G Peddapatla, M Rizwan Ahmed, Caroline A Blackshields, M J Sousa-Gallagher, Sean McSweeney, J Krüse, Abina M Crean, Dara Fitzpatrick
The ability of Broadband Acoustic Resonance Dissolution Spectroscopy (BARDS) to assess the wettability of powder blends is investigated. BARDS is a novel analytical technology developed based on the change in acoustic phenomenon observed when material is added into a solvent under resonance. Addition of solid material to the solvent results in the introduction of gas (air) into the solvent, changing the compressibility of the solvent system and reducing the velocity of sound in the solvent. As a material is wetted and dissolved, the gas is released from the solvent and resonance frequency is altered...
September 19, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28926596/bifurcation-kinetics-of-drug-uptake-by-gram-negative-bacteria
#7
David A Westfall, Ganesh Krishnamoorthy, David Wolloscheck, Rupa Sarkar, Helen I Zgurskaya, Valentin V Rybenkov
Cell envelopes of many bacteria consist of two membranes studded with efflux transporters. Such organization protects bacteria from the environment and gives rise to multidrug resistance. We report a kinetic model that accurately describes the permeation properties of this system. The model predicts complex non-linear patterns of drug uptake complete with a bifurcation, which recapitulate the known experimental anomalies. We introduce two kinetic parameters, the efflux and barrier constants, which replace those of Michaelis and Menten for trans-envelope transport...
2017: PloS One
https://www.readbyqxmd.com/read/28926250/the-unimolecular-reactions-of-cf3chf2-studied-by-chemical-activation-assignment-of-rate-constants-and-threshold-energies-to-the-1-2-h-atom-transfer-1-1-hf-elimination-and-1-2-hf-elimination-reactions-and-the-dependence-of-threshold-energies-on-the-number-of
#8
Caleb A Smith, Blanton R Gillespie, George L Heard, Donald W Setser, Bert E Holmes
The recombination of CF3 and CHF2 radicals in a room temperature bath gas was used to prepare CF3CHF2* molecules with 101 kcal mol-1 of vibrational energy. The subsequent 1,2-H-atom transfer, 1,1-HF elimination and 1,2-HF elimination reactions were observed as a function of bath gas pressure by following the CHF3, CF3(F)C: and C2F4 product concentrations by gas chromatography using a mass spectrometer as the detector. The singlet CF3(F)C: concentration was measured by trapping the carbene with trans-2-butene...
September 19, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28925687/film-confinement-induced-jump-percolation-wetting-transition-in-amphiphilic-block-copolymer-films
#9
Namrata Salunke, Asritha Nallapaneni, Guangcui Yuan, Christopher M Stafford, Hui Niu, Matthew D Shawkey, Robert A Weiss, Alamgir Karim
We report a first-order like sharp surface wettability transition with varying film thickness dependent morphology in cast films of an amphiphilic triblock copolymer. Films comprised of poly(2-(N-ethylperfluotooctanesulfonamido) ethyl methylacrylate), poly(FOSM), and poly(N,N'-dimethyl acrylamide), poly(DMA), with thickness (h) in the transition-range, 200 < h < 300 nm, exhibited an abrupt hydrophobic to hydrophilic dynamic water contact angle transition. After an induction time, ti ≈ 40 to 180 s, water contact angle varied as θc ≈ 116° to 40° with an ultra-fast contact angle decay time constant, dθc/dt ≈ -18 °/s...
September 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28924426/changes-in-the-whole-blood-donor-population-in-south-west-germany-2010-versus-2016
#10
Michael Müller-Steinhardt, Christian Weidmann, Harald Klüter
BACKGROUND: In the recent past, the discrepancy between blood supply and future demand may have been overestimated. As medical progress develops rapidly, it will be essential to monitor ongoing demographic changes in the donor population regularly and to re-evaluate retention and recruiting strategies. The aim of the current study was to compare first-time donor (FTD) characteristics and their return rates. We therefore compared whole blood (WB) donations in total and the annual donation frequencies in 2010 and in 2015/2016...
August 2017: Transfusion Medicine and Hemotherapy
https://www.readbyqxmd.com/read/28924036/mechanisms-of-recognition-of-a%C3%AE-monomer-oligomer-and-fibril-by-homologous-antibodies
#11
Jun Zhao, Ruth Nussinov, Buyong Ma
Alzheimers' disease (AD) is one of the most devastating neurodegenerative diseases without effective therapies. Immunotherapy is a promising approach; but amyloidantibody structural information is limited. Here we simulate the recognition of monomeric, oligomeric, and fibril Aβ by three homologous antibodies (solanezumab, crenezumab, and their chimeric CreneFab). Solanezumab only binds the monomer, while crenezumab and CreneFab can recognize different oligomerization states; however, the structural basis for this observation is not understood...
September 18, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28923730/kinetics-analysis-of-interfacial-electron-transfer-processes-in-goethite-suspensions-systems
#12
Jianzhong Ma, Chengzhu Zhu, Jun Lu, Bin Ouyang, Qiaoqin Xie, Haibo Liu, Shuchuan Peng, Tianhu Chen
The photochemical behavior of goethite has been one of the most important topics in the field of environmental science due to it plays a significant role in the removal and transformation process of numerous pollutants. However, the interfacial electron transfer process of goethite is not clear. Using a nanosecond laser flash photolysis spectrometer, we report the transient spectroscopic observations of interfacial electron-transfer reactions in goethite dispersion under UV irradiation. Excitation of goethite generated conduction-band electron (ecb(-)) and hole (h(+))...
September 7, 2017: Chemosphere
https://www.readbyqxmd.com/read/28923113/chinese-version-of-the-constant-murley-questionnaire-for-shoulder-pain-and-disability-a-reliability-and-validation-study
#13
Min Yao, Long Yang, Zuo-Yuan Cao, Shao-Dan Cheng, Shuang-Lin Tian, Yue-Li Sun, Jing Wang, Bao-Ping Xu, Xiao-Chun Hu, Yong-Jun Wang, Ying Zhang, Xue-Jun Cui
BACKGROUND: Shoulder pain is a common musculoskeletal disorder in Chinese population, which affects more than 1,3 billion individuals. To the best of our knowledge, there has been no available Chinese-language version of measurements of shoulder pain and disability so far. Moreover, the Constant-Murley score (CMS) questionnaire is a universally recognized patient-reported questionnaire for clinical practice and research. The present study was designed to evaluate a Chinese translational version of CMS and subsequently assess its reliability and validity...
September 18, 2017: Health and Quality of Life Outcomes
https://www.readbyqxmd.com/read/28922682/the-energy-cost-of-running-with-the-ball-in-soccer
#14
Alessandro Piras, Milena Raffi, Charalampos Atmatzidis, Franco Merni, Rocco Di Michele
Running with the ball is a soccer-specific activity frequently used by players during match play and training drills. Nevertheless, the energy cost (EC) of on-grass running with the ball has not yet been determined. The purpose of this study was therefore to assess the EC of constant-speed running with the ball, and to compare it with the EC of normal running. Eight amateur soccer players performed two 6- min runs at 10 km/h on artificial turf, respectively with and without the ball. EC was measured with indirect calorimetry and, furthermore, estimated with a method based on players' accelerations measured with a GPS receiver...
September 18, 2017: International Journal of Sports Medicine
https://www.readbyqxmd.com/read/28922608/j-si-h-coupling-constants-of-activated-si-h-bonds
#15
Petra Meixner, Kilian Batke, Andreas Fischer, Dominik Schmitz, Georg Eickerling, Marcel Kalter, Klaus Ruhland, Klaus Eichele, José E Barquera-Lozada, Nicola P M Casati, Fabio Montisci, Piero Macchi, Wolfgang Scherer
We outline in this combined experimental and theoretical NMR study that sign and magnitude of J(Si,H) coupling constants provide reliable indicators to evaluate the extent of the oxidative addition of Si-H bonds in hydrosilane complexes. In combination with experimental electron density studies and MO analyses a simple structure-property relationship emerges: positive J(Si,H) coupling constants are observed in cases where M → L π-back-donation (M = transition metal; L = hydrosilane ligand) dominates. The corresponding complexes are located close to the terminus of the respective oxidative addition trajectory...
September 18, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28922543/in-vitro-and-in-vivo-degradation-of-microfiber-bioresorbable-coronary-scaffold
#16
Chi-Hung Huang, Sheng-Yang Lee, Sonida Horng, Louis-Georges Guy, Ting-Bin Yu
The degradation of Mirage Bioresorbable Microfiber Scaffold was evaluated in vitro and in vivo. The degradation in polymer molecular weight (MW), strut morphology, and integrity was accessed using gel permeation chromatography (GPC), X-ray micro-computed tomography (micro-CT) evaluation. To simulate the physiological degradation in vitro, scaffolds were deployed in silicone mock vessels connected to a peristaltic pumping system, which pumps 37°C phosphate-buffered saline (PBS, pH 7.4) at a constant rate. At various time points (30D, 60D, 90D, 180D, 270D, and 360D), the MW of microfibers decreased to 57...
September 18, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28921992/a-mechanistic-understanding-of-the-synergistic-potential-of-azole-fungicides-in-the-aquatic-invertebrate-gammarus-pulex
#17
Andrea Rösch, Michele Gottardi, Caroline Vignet, Nina Cedergreen, Juliane Hollender
Azole fungicides are known inhibitors of the important enzyme class cytochrome P450 monooxygenases (CYPs), thereby influencing the detoxification of co-occurring substances via biotransformation. This synergism in mixtures containing an azole has mostly been studied by effect measurements, while the underlying mechanism has been less well investigated. In this study, six azole fungicides (cyproconazole, epoxiconazole, ketoconazole, prochloraz, propiconazole and tebuconazole) were selected to investigate their synergistic potential and their CYP inhibition strength in the aquatic invertebrate Gammarus pulex...
September 18, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28921959/quantification-and-theoretical-analysis-of-the-electrophilicities-of-michael-acceptors
#18
Dominik S Allgäuer, Harish Jangra, Haruyasu Asahara, Zhen Li, Quan Chen, Hendrik Zipse, Armin R Ofial, Herbert Mayr
In order to quantify the electrophilic reactivities of common Michael acceptors, we measured the kinetics of the reactions of monoacceptor-substituted ethylenes (H2C═CH-Acc, 1) and styrenes (PhCH═CH-Acc, 2) with pyridinium ylides 3, sulfonium ylide 4, and sulfonyl-substituted chloromethyl anion 5. Substitution of the 57 measured second-order rate constants (log k) and the previously reported nucleophile-specific parameters N and sN for 3-5 into the correlation log k = sN(E + N) allowed us to calculate 15 new empirical electrophilicity parameters E for Michael acceptors 1 and 2...
September 18, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28921217/effect-of-lower-and-higher-alcohol-fuel-synergies-in-biofuel-blends-and-exhaust-treatment-system-on-emissions-from-ci-engine
#19
Thiyagarajan Subramanian, Edwin Geo Varuvel, Leenus Jesu Martin, Nagalingam Beddhannan
The present study deals with performance, emission and combustion studies in a single cylinder CI engine with lower and higher alcohol fuel synergies with biofuel blends and exhaust treatment system. Karanja oil methyl ester (KOME), widely available biofuel in India, and orange oil (ORG), a low carbon biofuel, were taken for this study, and equal volume blend was prepared for testing. Methanol (M) and n-pentanol (P) was taken as lower and higher alcohol and blended 20% by volume with KOME-ORG blend. Activated carbon-based exhaust treatment indigenous system was designed and tested with KOME-ORG + M20 and KOME-ORG + P20 blend...
September 18, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28920752/treatment-of-synthetic-refinery-wastewater-in-anoxic-aerobic-sequential-moving-bed-reactors-and-sulphur-recovery
#20
Subrat Kumar Mallick, Saswati Chakraborty
Objective of the present study was to simultaneously biodegrade synthetic petroleum refinery wastewater containing phenol (750 mg/L), sulphide (750 mg/L), hydrocarbon (as emulsified diesel of 300 mg/L), ammonia-nitrogen (350 mg/L) at pH >9 in anoxic-aerobic sequential moving bed reactors. The optimum mixing speed of anoxic reactor was observed at 20 rpm and beyond that, removal rate remained constant. In anoxic reactor the minimum hydraulic retention time was observed to be 2 days for complete removal of sulphide, 40-50% removal of phenol and total hydrocarbons and 52% of sulphur recovery...
September 18, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
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