Colwyn A Headley, Shalini Gautam, Angelica Olmo-Fontanez, Andreu Garcia-Vilanova, Varun Dwivedi, Anwari Akhter, Alyssa Schami, Kevin Chiem, Russell Ault, Hao Zhang, Hong Cai, Alison Whigham, Jennifer Delgado, Amberlee Hicks, Philip S Tsao, Jonathan Gelfond, Luis Martinez-Sobrido, Yufeng Wang, Jordi B Torrelles, Joanne Turner
Mitochondrial dysfunction alters cellular metabolism, increases tissue oxidative stress, and may be principal to the dysregulated signaling and function of CD4+ T lymphocytes in the elderly. In this proof of principle study, it is investigated whether the transfer of functional mitochondria into CD4+ T cells that are isolated from old mice (aged CD4+ T cells), can abrogate aging-associated mitochondrial dysfunction, and improve the aged CD4+ T cell functionality. The results show that the delivery of exogenous mitochondria to aged non-activated CD4+ T cells led to significant mitochondrial proteome alterations highlighted by improved aerobic metabolism and decreased cellular mitoROS...
November 21, 2023: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)