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https://www.readbyqxmd.com/read/28419111/time-resolved-and-label-free-monitoring-of-extracellular-metabolites-by-surface-enhanced-raman-spectroscopy
#1
Victoria Shalabaeva, Laura Lovato, Rosanna La Rocca, Gabriele C Messina, Michele Dipalo, Ermanno Miele, Michela Perrone, Francesco Gentile, Francesco De Angelis
Metabolomics is an emerging field of cell biology that aims at the comprehensive identification of metabolite levels in biological fluids or cells in a specific functional state. Currently, the major tools for determining metabolite concentrations are mass spectrometry coupled with chromatographic techniques and nuclear magnetic resonance, which are expensive, time consuming and destructive for the samples. Here, we report a time resolved approach to monitor metabolite dynamics in cell cultures, based on Surface Enhanced Raman Scattering (SERS)...
2017: PloS One
https://www.readbyqxmd.com/read/28398342/two-subgroups-of-antipsychotic-naive-first-episode-schizophrenia-patients-identified-with-a-gaussian-mixture-model-on-cognition-and-electrophysiology
#2
N Bak, B H Ebdrup, B Oranje, B Fagerlund, M H Jensen, S W Düring, M Ø Nielsen, B Y Glenthøj, L K Hansen
Deficits in information processing and cognition are among the most robust findings in schizophrenia patients. Previous efforts to translate group-level deficits into clinically relevant and individualized information have, however, been non-successful, which is possibly explained by biologically different disease subgroups. We applied machine learning algorithms on measures of electrophysiology and cognition to identify potential subgroups of schizophrenia. Next, we explored subgroup differences regarding treatment response...
April 11, 2017: Translational Psychiatry
https://www.readbyqxmd.com/read/28363269/establishing-a-large-animal-model-for-in-vivo-reprogramming-of-bile-duct-cells-into-insulin-secreting-cells-to-treat-diabetes
#3
Caitlin Marie Hill, Anannya Banga, Juan E Abrahante, Ce Yuan, Lucas A Mutch, Jody Janecek, Timothy O'Brien, Melanie L Graham, James Dutton
Type I diabetes manifests in autoimmune destruction of beta cells requiring metabolic management with an exogenous replacement source of insulin, either by repeated injection of recombinant insulin or by transplantation of allogenic islets from cadaveric donors. Both of these approaches have severe limitations. Repeated insulin injection requires intensive blood glucose monitoring, is expensive, and is associated with decreased quality of life measures. Islet transplant, while highly effective, is severely limited by shortage of donor organs...
March 31, 2017: Human Gene Therapy. Clinical Development
https://www.readbyqxmd.com/read/28287094/enlightening-discriminative-network-functional-modules-behind-principal-component-analysis-separation-in-differential-omic-science-studies
#4
Sara Ciucci, Yan Ge, Claudio Durán, Alessandra Palladini, Víctor Jiménez-Jiménez, Luisa María Martínez-Sánchez, Yuting Wang, Susanne Sales, Andrej Shevchenko, Steven W Poser, Maik Herbig, Oliver Otto, Andreas Androutsellis-Theotokis, Jochen Guck, Mathias J Gerl, Carlo Vittorio Cannistraci
Omic science is rapidly growing and one of the most employed techniques to explore differential patterns in omic datasets is principal component analysis (PCA). However, a method to enlighten the network of omic features that mostly contribute to the sample separation obtained by PCA is missing. An alternative is to build correlation networks between univariately-selected significant omic features, but this neglects the multivariate unsupervised feature compression responsible for the PCA sample segregation...
March 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28272319/facilitated-visual-interpretation-of-scores-in-principal-component-analysis-by-bioactivity-labeling-of-1h-nmr-spectra-metabolomics-investigation-and-identification-of-a-new-%C3%AE-glucosidase-inhibitor-in-radix-astragali
#5
Yueqiu Liu, Nils T Nyberg, Anna K Jäger, Dan Staerk
Radix Astragali is a component of several traditional medicines used for the treatment of type 2 diabetes in China. Radix Astragali is known to contain isoflavones, which inhibit α-glucosidase in the small intestines, and thus lowers the blood glucose levels. In this study, 21 samples obtained from different regions of China were extracted with ethyl acetate, then the IC50-values were determined, and the crude extracts were analyzed by 1H-NMR spectroscopy. A principal component analysis of the 1H-NMR spectra labeled with their IC50-values, that is, bioactivity-labeled 1H-NMR spectra, showed a clear correlation between spectral profiles and the α-glucosidase inhibitory activity...
March 6, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28193066/proof-of-principle-of-rtlc-an-open-source-software-developed-for-image-evaluation-and-multivariate-analysis-of-planar-chromatograms
#6
Dimitri Fichou, Petar Ristivojević, Gertrud E Morlock
High-performance thin-layer chromatography (HPTLC) is an advantageous analytical technique for analysis of complex samples. Combined with multivariate data analysis, it turns out to be a powerful tool for profiling of many samples in parallel. So far, chromatogram analysis has been time-consuming and required the application of at least two software packages to convert HPTLC chromatograms into a numerical data matrix. Hence, this study aimed to develop a powerful, all in one open-source software for user-friendly image processing and multivariate analysis of HPTLC chromatograms...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/28186705/current-and-future-perspectives-on-alginate-encapsulated-pancreatic-islet
#7
Berit L Strand, Abba E Coron, Gudmund Skjak-Braek
Transplantation of pancreatic islets in immune protective capsules holds the promise as a functional cure for type 1 diabetes, also about 40 years after the first proof of principal study. The concept is simple in using semipermeable capsules that allow the ingress of oxygen and nutrients, but limit the access of the immune system. Encapsulated human islets have been evaluated in four small clinical trials where the procedure has been evaluated as safe, but lacking long-term efficacy. Host reactions toward the biomaterials used in the capsules may be one parameter limiting the long-term function of the graft in humans...
April 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28170242/eu-iii-complex-with-do3a-amino-phosphonate-ligand-as-a-concentration-independent-ph-responsive-contrast-agent-for-magnetic-resonance-spectroscopy-mrs
#8
Tereza Krchová, Vít Herynek, Andrea Gálisová, Jan Blahut, Petr Hermann, Jan Kotek
A new DOTA-like ligand H5do3aNP with a 2-[amino(methylphosphonic acid)]ethyl-coordinating pendant arm was prepared, and its coordinating properties were studied by NMR spectroscopy and potentiometry. The study revealed a rare slow exchange (on the (1)H and (31)P NMR time scale) between protonated and unprotonated complex species with a corresponding acidity constant pKA ∼ 8.0. This unusually slow time scale associated with protonation is caused by a significant geometric change from square-antiprismatic (SA) arrangement observed for protonated complex SA-[Eu(Hdo3aNP)](-) to twisted-square-antiprismatic (TSA) arrangement found for deprotonated complex TSA-[Eu(do3aNP)](2-)...
February 20, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28124834/hybrid-network-model-for-deep-learning-of-chemical-data-application-to-antimicrobial-peptides
#9
Petra Schneider, Alex T Müller, Gisela Gabernet, Alexander L Button, Gernot Posselt, Silja Wessler, Jan A Hiss, Gisbert Schneider
We present a "deep" network architecture for chemical data analysis and classification together with a prospective proof-of-concept application. The model features a self-organizing map (SOM) as the input layer of a feedforward neural network. The SOM converts molecular descriptors to a two-dimensional image for further processing. We implemented lateral neuron inhibition for contrast enhancement. The model achieved improved classification accuracy and predictive robustness compared to feedforward network classifiers lacking the SOM layer...
January 2017: Molecular Informatics
https://www.readbyqxmd.com/read/28088244/study-of-the-variability-of-scapular-inclination-and-the-glenoid-version-considerations-for-preoperative-planning-clinical-radiological-study
#10
Petr Fulin, Martin Kysilko, David Pokorny, Radek Padr, Nikola Kasprikova, Ivan Landor, Antonin Sosna
BACKGROUND: Preoperative planning with the aid of imaging methods is a principal factor in successful surgery on the shoulder. This work aims to evaluate the variability of glenoid version, spiralling twist and scapular inclination in relation to the frontal axis. Studies focusing on measuring the variability of scapular inclination in the standardised rest position are lacking in the literature. METHODS: We evaluated 104 CT scans of the shoulder. We measured the glenoid version with respect to the scapular axis at three levels...
January 14, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/28081583/-early-results-of-adjuvant-topical-treatment-of-recurrent-osteomyelitis-with-absorbable-antibiotic-carriers
#11
Y Gramlich, G Walter, J Gils, R Hoffmann
Background Treatment of musculoskeletal infections principally consists of radical surgical debridement and systemic administration of antibiotics. Additional local antibiotic therapy is not yet generally established, and lacks evidence-based proof of efficacy. Nonetheless, there are a variety of practical approaches, as most specialised departments are unwilling to forego this option. The established polymethylmetacrylate (PMMA) carrier system has a number of practical disadvantages. This has led to the increased use of absorbable carrier systems, and those based on calcium sulphate have given particularly encouraging results...
February 2017: Zeitschrift Für Orthopädie und Unfallchirurgie
https://www.readbyqxmd.com/read/28054957/molecular-biomarkers-for-prediction-of-targeted-therapy-response-in-metastatic-breast-cancer-trick-or-treat
#12
REVIEW
Angela Toss, Marta Venturelli, Chiara Peterle, Federico Piacentini, Stefano Cascinu, Laura Cortesi
In recent years, the study of genomic alterations and protein expression involved in the pathways of breast cancer carcinogenesis has provided an increasing number of targets for drugs development in the setting of metastatic breast cancer (i.e., trastuzumab, everolimus, palbociclib, etc.) significantly improving the prognosis of this disease. These drugs target specific molecular abnormalities that confer a survival advantage to cancer cells. On these bases, emerging evidence from clinical trials provided increasing proof that the genetic landscape of any tumor may dictate its sensitivity or resistance profile to specific agents and some studies have already showed that tumors treated with therapies matched with their molecular alterations obtain higher objective response rates and longer survival...
January 4, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28047240/we-ab-207b-07-dose-cloud-generating-big-data-for-radiation-therapy-treatment-plan-optimization-research
#13
M M Folkerts, T Long, R J Radke, Z Tian, X Jia, M Chen, W Lu, S B Jiang
PURPOSE: To provide a tool to generate large sets of realistic virtual patient geometries and beamlet doses for treatment optimization research. This tool enables countless studies exploring the fundamental interplay between patient geometry, objective functions, weight selections, and achievable dose distributions for various algorithms and modalities. METHODS: Generating realistic virtual patient geometries requires a small set of real patient data. We developed a normalized patient shape model (PSM) which captures organ and target contours in a correspondence-preserving manner...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28009592/assessment-of-acute-trauma-exposure-response-for-fire-ems-personnel
#14
Melissa C Hoffmann
PURPOSE: The purpose of this study was to develop an instrument that measures response to acute trauma exposure for firefighter and emergency medical service (EMS) personnel. The Acute Trauma Exposure Response Scale (ATERS) was intended to assess firefighter and EMS personnel response to acute trauma exposure from analytical, emotional, and physical perspectives. METHODS: Data were analyzed on 97 firefighter and EMS personnel employed by a fire department in a mid-sized city in a western state...
2016: Journal of Applied Measurement
https://www.readbyqxmd.com/read/27958241/psychometric-properties-of-a-turkish-version-of-the-oral-health-impact-profile-14
#15
N Balci, N Alkan, C A Gurgan
OBJECTIVES: The purpose of this study was to analyze and evaluate a Turkish translation of the oral health impact profile-14 (OHIP-14) in a Turkish population to provide an objective standard for future studies. METHODS: This cross-sectional research study consisted of three independent studies. Data were collected utilizing a personal interview and a review of periodontal records. This study was performed on 1205 subjects who were visiting for routine medical check-ups...
January 2017: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/27885707/spatial-resolution-and-velocity-field-improvement-of-4d-flow-mri
#16
Fraser M Callaghan, Stuart M Grieve
PURPOSE: 4D-flow MRI obtains a time-dependent 3D velocity field; however, its use for the calculation of higher-order parameters is limited by noise. We present an algorithm for denoising 4D-flow data. THEORY AND METHODS: By integrating a velocity field and eliminating streamlines in noisy flow, depicted by high curvature, a denoised dataset may be extracted. This method, defined as the velocity field improvement (VFIT) algorithm, was validated in an analytical dataset and using in vivo data in comparison with a computation fluid dynamics (CFD) simulation...
November 24, 2016: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/27872098/systematic-drug-screening-identifies-tractable-targeted-combination-therapies-in-triple-negative-breast-cancer
#17
Vikram B Wali, Casey G Langdon, Matthew A Held, James T Platt, Gauri A Patwardhan, Anton Safonov, Bilge Aktas, Lajos Pusztai, David F Stern, Christos Hatzis
Triple-negative breast cancer (TNBC) remains an aggressive disease without effective targeted therapies. In this study, we addressed this challenge by testing 128 FDA-approved or investigational drugs as either single agents or in 768 pairwise drug combinations in TNBC cell lines to identify synergistic combinations tractable to clinical translation. Medium-throughput results were scrutinized and extensively analyzed for sensitivity patterns, synergy, anticancer activity, and were validated in low-throughput experiments...
January 15, 2017: Cancer Research
https://www.readbyqxmd.com/read/27836429/functional-and-effective-brain-connectivity-for-discrimination-between-alzheimer-s-patients-and-healthy-individuals-a-study-on-resting-state-eeg-rhythms
#18
Katarzyna J Blinowska, Franciszek Rakowski, Maciej Kaminski, Fabrizio De Vico Fallani, Claudio Del Percio, Roberta Lizio, Claudio Babiloni
OBJECTIVE: This exploratory study provided a proof of concept of a new procedure using multivariate electroencephalographic (EEG) topographic markers of cortical connectivity to discriminate normal elderly (Nold) and Alzheimer's disease (AD) individuals. METHOD: The new procedure was tested on an existing database formed by resting state eyes-closed EEG data (19 exploring electrodes of 10-20 system referenced to linked-ear reference electrodes) recorded in 42 AD patients with dementia (age: 65...
April 2017: Clinical Neurophysiology: Official Journal of the International Federation of Clinical Neurophysiology
https://www.readbyqxmd.com/read/27832353/a-proof-of-principal-study-on-the-use-of-direct-pcr-of-semen-and-spermatozoa-and-development-of-a-differential-isolation-protocol-for-use-in-cases-of-alleged-sexual-assault
#19
Shanan S Tobe, Yuvaneswari C Swaran, Lynn Dennany, Ursula Sibbing, Kristina Schulze Johann, Lindsey Welch, Marielle Vennemann
Sexual assault samples are some of the most common samples encountered in forensic analysis. These samples can require a significant time investment due to differential extraction processes. We report on the first record of successful direct amplification of semen for STR analysis. Neat seminal fluid, dilutions ranging from 1:5 to 1:160 and GEDNAP samples were successfully amplified using a direct method. A mild differential isolation technique to enrich spermatozoa was developed and successfully implemented to separate and directly amplify a mixture of semen and female epithelial cells...
January 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/27770634/repair-of-bone-defects-in%C3%A2-vivo-using-tissue-engineered-hypertrophic-cartilage-grafts-produced-from-nasal-chondrocytes
#20
Katie Bardsley, Agnieska Kwarciak, Christine Freeman, Ian Brook, Paul Hatton, Aileen Crawford
The regeneration of large bone defects remains clinically challenging. The aim of our study was to use a rat model to use nasal chondrocytes to engineer a hypertrophic cartilage tissue which could be remodelled into bone in vivo by endochondral ossification. Primary adult rat nasal chondrocytes were isolated from the nasal septum, the cell numbers expanded in monolayer culture and the cells cultured in vitro on polyglycolic acid scaffolds in chondrogenic medium for culture periods of 5-10 weeks. Hypertrophic differentiation was assessed by determining the temporal expression of key marker genes and proteins involved in hypertrophic cartilage formation...
October 11, 2016: Biomaterials
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