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https://www.readbyqxmd.com/read/28334731/characterisation-of-cct271850-a-selective-oral-and-potent-mps1-inhibitor-used-to-directly-measure-in-vivo-mps1-inhibition-vs-therapeutic-efficacy
#1
Amir Faisal, Grace W Y Mak, Mark D Gurden, Cristina P R Xavier, Simon J Anderhub, Paolo Innocenti, Isaac M Westwood, Sébastien Naud, Angela Hayes, Gary Box, Melanie R Valenti, Alexis K De Haven Brandon, Lisa O'Fee, Jessica Schmitt, Hannah L Woodward, Rosemary Burke, Rob L M vanMontfort, Julian Blagg, Florence I Raynaud, Suzanne A Eccles, Swen Hoelder, Spiros Linardopoulos
BACKGROUND: The main role of the cell cycle is to enable error-free DNA replication, chromosome segregation and cytokinesis. One of the best characterised checkpoint pathways is the spindle assembly checkpoint, which prevents anaphase onset until the appropriate attachment and tension across kinetochores is achieved. MPS1 kinase activity is essential for the activation of the spindle assembly checkpoint and has been shown to be deregulated in human tumours with chromosomal instability and aneuploidy...
March 23, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28334186/rnascclust-clustering-rna-sequences-using-structure-conservation-and-graph-based-motifs
#2
Milad Miladi, Alexander Junge, Fabrizio Costa, Stefan E Seemann, Jakob Hull Havgaard, Jan Gorodkin, Rolf Backofen
Motivation: Clustering RNA sequences with common secondary structure is an essential step towards studying RNA function. Whereas structural RNA alignment strategies typically identify common structure for orthologous structured RNAs, clustering seeks to group paralogous RNAs based on structural similarities. However, existing approaches for clustering paralogous RNAs, do not take the compensatory base pair changes obtained from structure conservation in orthologous sequences into account...
February 27, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28333117/fiber-optic-sensor-embedment-study-for-multi-parameter-strain-sensing
#3
Monssef Drissi-Habti, Venkadesh Raman, Aghiad Khadour, Safiullah Timorian
The fiber optic sensors (FOSs) are commonly used for large-scale structure monitoring systems for their small size, noise free and low electrical risk characteristics. Embedded fiber optic sensors (FOSs) lead to micro-damage in composite structures. This damage generation threshold is based on the coating material of the FOSs and their diameter. In addition, embedded FOSs are aligned parallel to reinforcement fibers to avoid micro-damage creation. This linear positioning of distributed FOS fails to provide all strain parameters...
March 23, 2017: Sensors
https://www.readbyqxmd.com/read/28328013/validation-of-surface-to-volume-ratio-measurements-derived-from-oscillating-gradient-spin-echo-on-a-clinical-scanner-using-anisotropic-fiber-phantoms
#4
Gregory Lemberskiy, Steven H Baete, Martijn A Cloos, Dmitry S Novikov, Els Fieremans
A diffusion measurement in the short-time surface-to-volume ratio (S/V) limit (Mitra et al., Phys Rev Lett. 1992;68:3555) can disentangle the free diffusion coefficient from geometric restrictions to diffusion. Biophysical parameters, such as the S/V of tissue membranes, can be used to estimate microscopic length scales non-invasively. However, due to gradient strength limitations on clinical MRI scanners, pulsed gradient spin echo (PGSE) measurements are impractical for probing the S/V limit. To achieve this limit on clinical systems, an oscillating gradient spin echo (OGSE) sequence was developed...
March 22, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/28326907/skin-sensitizers-in-cosmetics-and-beyond-potential-multiple-mechanisms-of-action-and-importance-of-t-cell-assays-for-in-vitro-screening
#5
Stanislav Vukmanović, Nakissa Sadrieh
Allergic contact dermatitis (ACD) is a delayed-type hypersensitivity (DTH) reaction induced by repeated contact with sensitizers. The ability of a chemical to act as a sensitizer has most frequently been tested in animals. As the use of animals for these purposes is gradually and globally being phased out, there is a need for reliable in vitro surrogate assays. Currently proposed in vitro assays are designed to test four key events of the adverse outcome pathway (AOP) involving covalent modification of self-proteins by sensitizers (haptenation) and presentation of new antigens (hapten/carrier complexes) to the immune system...
March 22, 2017: Critical Reviews in Toxicology
https://www.readbyqxmd.com/read/28324166/redescription-of-chloromyxum-ellipticum-li-nie-1973-myxosporea-chloromyxidae-infecting-the-gall-bladder-of-grass-carp-ctenopharyngodon-idellus-valenciennes-1844-supplemented-by-morphological-and-molecular-characteristics
#6
J Y Zhang, Y L Zhao, M D Batueva, D Luo, Z F Xing, Q Q Zhang, X H Liu
The traditional taxonomy of the genus Chloromyxum Mingazzini, 1890 has been intensively challenged to be paraphyletic by recent ribosomal DNA (rDNA)-based phylogenetic analysis. Undersampling to get rich sequence data to infer more scientific phylogenetic relationships makes scientists conservatively assign all non-marine elasmobranch-infecting species as Chloromyxum sensu lato. Although complex ridge pattern on the spore surface observed by scanning electron microscopy was thought to be critical for the identification of Chloromyxum species, insufficient data also prevent this ultrastructural data to be a valid taxonomic feature for this genus...
March 21, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28321805/introduction-of-a-computer-based-method-for-automated-planning-of-reduction-paths-under-consideration-of-simulated-muscular-forces
#7
Jan Buschbaum, Rainer Fremd, Tim Pohlemann, Alexander Kristen
PURPOSE: Reduction is a crucial step in the surgical treatment of bone fractures. Finding an optimal path for restoring anatomical alignment is considered technically demanding because collisions as well as high forces caused by surrounding soft tissues can avoid desired reduction movements. The repetition of reduction movements leads to a trial-and-error process which causes a prolonged duration of surgery. By planning an appropriate reduction path-an optimal sequence of target-directed movements-these problems should be overcome...
March 20, 2017: International Journal of Computer Assisted Radiology and Surgery
https://www.readbyqxmd.com/read/28319898/immunodetection-of-salivary-biomarkers-by-an-optical-microfluidic-biosensor-with-polyethylenimine-modified-polythiophene-c70-organic-photodetectors
#8
Tao Dong, Nuno Miguel Matos Pires
This work reports a novel optical microfluidic biosensor with highly sensitive organic photodetectors (OPDs) for absorbance-based detection of salivary protein biomarkers at the point of care. The compact and miniaturized biosensor has comprised OPDs made of polythiophene-C70 bulk heterojunction for the photoactive layer; whilst a calcium-free cathode interfacial layer, made of linear polyethylenimine, was incorporated to the photodetectors to enhance the low cost. The OPDs realized onto a glass chip were aligned to antibody-functionalized chambers of a poly(methyl methacrylate) microfluidic chip, in where immunogold-silver assays were conducted...
March 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28315407/controlled-delivery-of-platelet-derived-proteins-enhances-porcine-wound-healing
#9
Daniel W Long, Noah R Johnson, Eric M Jeffries, Hidetaka Hara, Yadong Wang
Platelet-rich plasma (PRP) is widely used for many clinical indications including wound healing due to the high concentrations of growth factors. However, the short half-life of these therapeutic proteins requires multiple large doses, and their efficacy is highly debated among clinicians. Here we report a method of protecting these proteins and releasing them in a controlled manner via a heparin-based coacervate delivery vehicle to improve wound healing in a porcine model. Platelet-derived proteins incorporated into the coacervate were protected and slowly released over 3weeks in vitro...
March 14, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28301134/oxygen-vacancies-in-shape-controlled-cu2o-reduced-graphene-oxide-in2o3-hybrid-for-promoted-photocatalytic-water-oxidation-and-degradation-of-environmental-pollutants
#10
Jie Liu, Jun Ke, Degang Li, Hongqi Sun, Ping Liang, Xiaoguang Duan, Wenjie Tian, Moses O Tade, Shaomin Liu, Shaobin Wang
A novel shape controlled Cu2O/reduced graphene oxide/In2O3 (Cu2O/RGO/In2O3) hybrid with abundant oxygen vacancies was prepared by a facile, surfactant-free method. The hybrid photocatalyst exhibits an increased photocatalytic activity in water oxidation and degradation of environmental pollutants (methylene blue and Cr6+ solutions) compared with pure In2O3 and Cu2O materials. The presence of oxygen vacancies in Cu2O/RGO/In2O3 and the formation of heterojunction between In2O3 and Cu2O induce extra diffusive electronic states above the valence band (VB) edge and reduce the band gap of the hybrid consequently...
March 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28300202/thermoalign-a-genome-aware-primer-design-tool-for-tiled-amplicon-resequencing
#11
Felix Francis, Michael D Dumas, Randall J Wisser
Isolating and sequencing specific regions in a genome is a cornerstone of molecular biology. This has been facilitated by computationally encoding the thermodynamics of DNA hybridization for automated design of hybridization and priming oligonucleotides. However, the repetitive composition of genomes challenges the identification of target-specific oligonucleotides, which limits genetics and genomics research on many species. Here, a tool called ThermoAlign was developed that ensures the design of target-specific primer pairs for DNA amplification...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28296898/a-new-survey-to-evaluate-conflict-of-interest-policies-at-academic-medical-centers
#12
Marcia Hams, Wells G Wilkinson, Lynn Zentner, Cory Schmidt, Raed A Dweik, Matthew Karafa, Susannah L Rose
BACKGROUND: A majority of academic medical centers (AMCs) have now adopted conflict of interest policies (COI) to address relationships with pharmaceutical and device industries that can increase the risk of bias in patient care, education and research. However, AMCs may have little information on the impact of their policies. This paper provides a new method, which is a free, publicly-available survey, to fill this information gap and improve COI programs at AMCs. METHODS & FINDINGS: The survey, piloted in three AMCs and designed in collaboration with national conflicts of interest policy experts, covers a range of universal compliance-related concerns, which allows institutions to tailor questions to align with their own policies and culture...
2017: PloS One
https://www.readbyqxmd.com/read/28295727/new-mo6-te6-sub-nanometer-diameter-nanowire-phase-from-2h-mote2
#13
Hui Zhu, Qingxiao Wang, Chenxi Zhang, Rafik Addou, Kyeongjae Cho, Robert M Wallace, Moon J Kim
A novel phase transition, from multilayered 2H-MoTe2 to a parallel bundle of sub-nanometer-diameter metallic Mo6 Te6 nanowires (NWs) driven by catalyzer-free thermal-activation (400-500 °C) under vacuum, is demonstrated. The NWs form along the 〈11-20〉 2H-MoTe2 crystallographic directions with lengths in the micrometer range. The metallic NWs can act as an efficient hole injection layer on top of 2H-MoTe2 due to favorable band-alignment. In particular, an atomically sharp MoTe2 /Mo6 Te6 interface and van der Waals gap with the 2H layers are preserved...
March 10, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28289998/slim-slit-lamp-image-mosaicing-handling-reflection-artifacts
#14
Kristina Prokopetc, Adrien Bartoli
PURPOSE: The slit lamp is an essential instrument for eye care. It is used in navigated laser treatment with retina mosaicing to assist diagnosis. Specifics of the imaging setup introduce bothersome illumination artifacts. They not only degrade the quality of the mosaic but may also affect the diagnosis. Existing solutions in SLIM manage to deal with strong glares which corrupt the retinal content entirely while leaving aside the correction of semitransparent specular highlights and lens flare...
March 13, 2017: International Journal of Computer Assisted Radiology and Surgery
https://www.readbyqxmd.com/read/28289437/best-hits-of-11110110111-model-free-selection-and-parameter-free-sensitivity-calculation-of-spaced-seeds
#15
Laurent Noé
BACKGROUND: Spaced seeds, also named gapped q-grams, gapped k-mers, spaced q-grams, have been proven to be more sensitive than contiguous seeds (contiguous q-grams, contiguous k-mers) in nucleic and amino-acid sequences analysis. Initially proposed to detect sequence similarities and to anchor sequence alignments, spaced seeds have more recently been applied in several alignment-free related methods. Unfortunately, spaced seeds need to be initially designed. This task is known to be time-consuming due to the number of spaced seed candidates...
2017: Algorithms for Molecular Biology: AMB
https://www.readbyqxmd.com/read/28288001/programmable-snapping-composites-with-bio-inspired-architecture
#16
Jascha U Schmied, Hortense Le Ferrand, Paolo Ermanni, André R Studart, Andres F Arrieta
The development of programmable self-shaping materials enables the onset of new and innovative functionalities in many application fields. Commonly, shape adaptation is achieved by exploiting diffusion-driven swelling or nano-scale phase transition, limiting the change of shape to slow motion predominantly determined by the environmental conditions and/or the materials specificity. To address these shortcomings, we report shape adaptable programmable shells that undergo morphing via a snap-through mechanism inspired by the Dionaea muscipula leaf, known as the Venus fly trap...
March 13, 2017: Bioinspiration & Biomimetics
https://www.readbyqxmd.com/read/28287090/controllable-conversion-of-quasi-freestanding-polymer-chains-to-graphene-nanoribbons
#17
Chuanxu Ma, Zhongcan Xiao, Honghai Zhang, Liangbo Liang, Jingsong Huang, Wenchang Lu, Bobby G Sumpter, Kunlun Hong, J Bernholc, An-Ping Li
In the bottom-up synthesis of graphene nanoribbons (GNRs) from self-assembled linear polymer intermediates, surface-assisted cyclodehydrogenations usually take place on catalytic metal surfaces. Here we demonstrate the formation of GNRs from quasi-freestanding polymers assisted by hole injections from a scanning tunnelling microscope (STM) tip. While catalytic cyclodehydrogenations typically occur in a domino-like conversion process during the thermal annealing, the hole-injection-assisted reactions happen at selective molecular sites controlled by the STM tip...
March 13, 2017: Nature Communications
https://www.readbyqxmd.com/read/28281438/engineered-microvasculature-in-pdms-networks-using-endothelial-cells-derived-from-human-induced-pluripotent-stem-cells
#18
Amogh Sivarapatna, Mahboobe Ghaedi, Yang Xiao, Edward Han, Binod Aryal, Jing Zhou, Carlos Fernandez-Hernando, Yibing Qyang, Karen K Hirschi, Laura E Niklason
In this study, we used a PDMS-based platform for the generation of intact, perfusioncompetent microvessel networks <i>in vitro</i>. COMSOL Multiphysics, a finite element analysis and simulation software package, was used to obtain simulated velocity, pressure, and shear stress profiles. Transgene-free human iPSCs were differentiated into partially arterialized endothelial cells (hiPSC-ECs) in 5 days under completely chemically defined conditions, using the small molecule GSK3β inhibitor CHIR99021 and thoroughly characterized for functionality and arteriallike marker expression...
March 9, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28277651/nanoengineering-of-core-shell-magnetic-mesoporous-microspheres-with-tunable-surface-roughness
#19
Qin Yue, Yu Zhang, Yongjian Jiang, Jialuo Li, Hongwei Zhang, Chengzhong Yu, Ahmed A Elzatahry, Abdulaziz Alghamdi, Yonghui Deng, Dongyuan Zhao
Functional core-shell mesoporous microspheres with integrated functions, controlled structure, and surface properties and morphologies have received increasing attention due to their excellent physicochemical properties. Herein, core-shell magnetic mesoporous materials with cauliflower-like morphology and tunable surface roughness have been synthesized through a kinetics-controlled interface co-assembly and deposition of mesostructured nanocomposites on Fe3O4@RF microspheres (RF refers to resorcinol formaldehyde resin)...
March 22, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28264954/value-based-insurance-design-benefit-offsets-reductions-in-medication-adherence-associated-with-switch-to-deductible-plan
#20
Mary E Reed, E Margaret Warton, Eileen Kim, Matthew D Solomon, Andrew J Karter
Enrollment in high-deductible health plans is increasing out-of-pocket spending. But innovative plans that pair deductibles with value-based insurance designs can help preserve low-cost access to high-value treatments for patients by aligning coverage with clinical value. Among adults in high-deductible health plans who were prescribed medications for chronic conditions, we examined what impact a value-based pharmacy benefit that offered free chronic disease medications had on medication adherence. Overall, we found that the value-based plan offset reductions in medication adherence associated with switching to a deductible plan...
March 1, 2017: Health Affairs
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