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leukemia inhibitory factor

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https://www.readbyqxmd.com/read/28928847/anti-angiogenic-effect-of-arsenic-trioxide-in-lung-cancer-via-inhibition-of-endothelial-cell-migration-proliferation-and-tube-formation
#1
Meng-Hang Yang, Ke-Jie Chang, Jin-Cheng Zheng, Hai Huang, Guang-Yuan Sun, Xue-Wei Zhao, Bing Li, Qing-Yu Xiu
Arsenic trioxide (As2O3) exhibits a remarkable effect on leukemia treatment; however, its effect on solid tumors remains poorly explored. The present study demonstrated the inhibitory effect of As2O3 on lung cancer and explored its possible mechanism. It was observed that As2O3 significantly inhibited the growth of lung cancer xenografts and tumor angiogenesis in vivo. The inhibitory effect of As2O3 on cell proliferation in vitro was more remarkable in vascular endothelial cells than in lung cancer cells. It was also observed that As2O3 inhibited the migration of vascular endothelial cells and disrupted vascular tube formation on Matrigel assays...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28906466/therapeutic-effect-of-exogenous-truncated-ik-protein-in-inflammatory-arthritis
#2
Seulgi Choi, HyeLim Park, SeoYeon Jung, Eun-Kyung Kim, Mi-La Cho, Jun-Ki Min, Su-Jin Moon, Sang-Myeong Lee, Jang-Hee Cho, Dong-Hee Lee, Jae-Hwan Nam
Inhibitor K562 (IK) protein was first isolated from the culture medium of K562, a leukemia cell line. It is known to be an inhibitory regulator of interferon-γ-induced major histocompatibility complex class (MHC) II expression. Previously, we found that transgenic (Tg) mice constitutively expressing truncated IK (tIK) showed reduced numbers of pathogenic Th1 and Th17 cells, which are known to be involved in the development of rheumatoid arthritis (RA). Here, we investigated whether exogenous tIK protein has a therapeutic effect in arthritis in disease models and analyzed its mechanism...
September 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28902633/ginkgetin-inhibits-proliferation-of-human-leukemia-cells-via-the-tnf-%C3%AE-signaling-pathway
#3
Ling-Ling Pan, Wen-Jun Wu, Gao-Feng Zheng, Xiao-Yan Han, Jing-Song He, Zhen Cai
Ginkgetin is known to be an anticancer agent in many studies. However, its effectiveness in treating chronic lymphoblastic leukemia (CLL) remains unknown. The present study aimed to evaluate the effects of ginkgetin on the growth of the K562 cell line. The MTT assay was employed to examine the proliferation of K562, and a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) staining was conducted to detect the apoptotic rates. Furthermore, changes of tumor necrosis factor-α (TNF-α) were detected by Western blot analysis...
September 13, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28901404/effects-of-anticholinergic-agent-on-mirna-profiles-and-transcriptomes-in-a-murine-model-of-allergic-rhinitis
#4
Minghua Hou, Wei Li, Zuozhong Xie, Jingang Ai, Bo Sun, Guolin Tan
Anticholinergic agent, ipratropium bromide (IB) ameliorates symptoms of allergic rhinitis (AR) using neuroimmunologic mechanisms. However, the underlying molecular mechanism remains largely unclear. In the present study, 27 mice with AR induced by ovalbumin were randomly allocated to one of three groups: Model group, model group with IB treatment for 2 weeks, and model group with IB treatment for 4 weeks. Allergic symptoms were evaluated according to symptoms scores. Differentially expressed genes [microRNAs (miRNAs) and messenger RNAs (mRNAs)] of nasal mucosa were identified by microarray analysis...
August 31, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28889080/reprogramming-of-rabbit-induced-pluripotent-stem-cells-toward-epiblast-and-chimeric-competency-using-kr%C3%A3-ppel-like-factors
#5
Yann Tapponnier, Marielle Afanassieff, Irène Aksoy, Maxime Aubry, Anaïs Moulin, Lucas Medjani, Wilhelm Bouchereau, Chloé Mayère, Pierre Osteil, Jazmine Nurse-Francis, Ioannis Oikonomakos, Thierry Joly, Luc Jouneau, Catherine Archilla, Barbara Schmaltz-Panneau, Nathalie Peynot, Harmonie Barasc, Alain Pinton, Jérome Lecardonnel, Elen Gocza, Nathalie Beaujean, Véronique Duranthon, Pierre Savatier
Rabbit induced pluripotent stem cells (rbiPSCs) possess the characteristic features of primed pluripotency as defined in rodents and primates. In the present study, we reprogrammed rbiPSCs using human Krüppel-like factors (KLFs) 2 and 4 and cultured them in a medium supplemented with fetal calf serum and leukemia inhibitory factor. These cells (designated rbEKA) were propagated by enzymatic dissociation for at least 30 passages, during which they maintained a normal karyotype. This new culturing protocol resulted in transcriptional and epigenetic reconfiguration, as substantiated by the expression of transcription factors and the presence of histone modifications associated with naïve pluripotency...
September 5, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28877726/the-effect-of-bovine-bst2a1-on-the-release-and-cell-to-cell-transmission-of-retroviruses
#6
Zhibin Liang, Yang Zhang, Jie Song, Hui Zhang, Suzhen Zhang, Yue Li, Juan Tan, Wentao Qiao
BACKGROUND: Human BST2 (hBST2, also called Tetherin) is a host restriction factor that blocks the release of various enveloped viruses. BST2s from different mammals also possess antiviral activity. Bovine BST2s (bBST2s), bBST2A1 and bBST2A2, reduce production of cell-free bovine leukemia virus (BLV) and vesicular stomatitis virus (VSV). However, the effect of bBST2 on other retroviruses remains unstudied. RESULTS: Here, we studied the antiviral activity of wildtype and mutant bBST2A1 proteins on retroviruses including human immunodeficiency virus type 1 (HIV-1), prototypic foamy virus (PFV), bovine foamy virus (BFV) and bovine immunodeficiency virus (BIV)...
September 6, 2017: Virology Journal
https://www.readbyqxmd.com/read/28874583/smad7-enables-stat3-activation-and-promotes-pluripotency-independent-of-tgf-%C3%AE-signaling
#7
Yi Yu, Shuchen Gu, Wenjian Li, Chuang Sun, Fenfang Chen, Mu Xiao, Lei Wang, Dewei Xu, Ye Li, Chen Ding, Zongping Xia, Yi Li, Sheng Ye, Pinglong Xu, Bin Zhao, Jun Qin, Ye-Guang Chen, Xia Lin, Xin-Hua Feng
Smad7 is a negative feedback product of TGF-β superfamily signaling and fine tunes a plethora of pleiotropic responses induced by TGF-β ligands. However, its noncanonical functions independent of TGF-β signaling remain to be elucidated. Here, we show that Smad7 activates signal transducers and activators of transcription 3 (STAT3) signaling in maintaining mouse embryonic stem cell pluripotency in a manner independent of the TGF-β receptors, yet dependent on the leukemia inhibitory factor (LIF) coreceptor glycoprotein 130 (gp130)...
September 5, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28872141/derivation-of-stem-cell-lines-from-mouse-preimplantation-embryos
#8
Marta Vila-Cejudo, Elena Ibañez, Josep Santalo
Mouse embryonic stem cell (mESC) derivation is the process by which pluripotent cell lines are established from preimplantation embryos. These lines retain the ability to either self-renew or differentiate under specific conditions. Due to these properties, mESC are a useful tool in regenerative medicine, disease modeling, and tissue engineering studies. This article describes a simple protocol to obtain mESC lines with high derivation efficiencies (60-80%) by culturing blastocysts from permissive mouse strains on feeder cells in defined medium supplemented with leukemia inhibitory factor...
August 20, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28865476/inhibition-of-hypothalamic-leukemia-inhibitory-factor-exacerbates-diet-induced-obesity-phenotype
#9
Milena Fioravante, Bruna Bombassaro, Albina F Ramalho, Nathalia R Dragano, Joseane Morari, Carina Solon, Natalia Tobar, Celso D Ramos, Licio A Velloso
BACKGROUND: The consumption of large amounts of dietary fats can trigger an inflammatory response in the hypothalamus and contribute to the dysfunctional control of caloric intake and energy expenditure commonly present in obesity. The objective of this study was to identify chemokine-related transcripts that could be involved in the early stages of diet-induced hypothalamic inflammation. METHODS: We used immunoblot, PCR array, real-time PCR, immunofluorescence staining, glucose and insulin tolerance tests, and determination of general metabolic parameters to evaluate markers of inflammation, body mass variation, and glucose tolerance in mice fed a high-fat diet...
September 2, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28845418/v64m-mutation-in-leukemia-inhibitory-factor-gene-in-women-infertility
#10
Gholamreza Niaei, Bita Amir Taghavi, Amir Niaei
No abstract text is available yet for this article.
July 2017: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/28836399/multiple-sclerosis-gene-therapy-with-recombinant-viral-vectors-overexpression-of-il-4-leukemia-inhibitory-factor-and-il-10-in-wharton-s-jelly-stem-cells-used-in-eae-mice-model
#11
Ahmad Hosseini, Hajar Estiri, Haleh Akhavan Niaki, Akram Alizadeh, Baharak Abdolhossein Zadeh, Sayyed Mohammad Hossein Ghaderian, Akbar Farjadfar, Ali Fallah
OBJECTIVES: Immunotherapy and gene therapy play important roles in modern medicine. The aim of this study is to evaluate the overexpression of interleukin-4 (IL-4), IL-10 and leukemia inhibitory factor (LIF) in Wharton's jelly stem cells (WJSCs) in the experimental autoimmune encephalomyelitis (EAE) mice model. MATERIALS AND METHODS: In this experimental study, a DNA construction containing IL- 4, IL-10 and LIF was assembled to make a polycistronic vector (as the transfer vector)...
October 2017: Cell Journal
https://www.readbyqxmd.com/read/28835937/leukemia-inhibitory-factor-increases-the-proliferation-of-human-endometrial-stromal-cells-and-expression-of-genes-related-to-pluripotency
#12
Mojdeh Salehnia, Mehri Fayazi, Shokreya Ehsani
BACKGROUND: Concerning the low population of human endometrial mesenchymal cells within the tissue and their potential application in the clinic and tissue engineering, some researches have been focused on their in vitro expansion. OBJECTIVE: The aim of this study was to evaluate the effect of leukemia inhibitory factor (LIF) as a proliferative factor on the expansion and proliferation of human endometrial stromal cells. MATERIALS AND METHODS: In this experimental study, the isolated and cultured human endometrial stromal cells from women at ovulatory phase aged 20-35 years, after fourth passage were divided into control and LIF-treated groups...
April 2017: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/28827150/the-role-of-the-leukemia-inhibitory-factor-receptor-in-neuroprotective-signaling
#13
REVIEW
Stephanie M Davis, Keith R Pennypacker
Several neurotropic cytokines relay their signaling through the leukemia inhibitory factor receptor. This 190kDa subunit couples with the 130kDa gp130 subunit to transduce intracellular signaling in neurons and oligodendrocytes that leads to expression of genes associated with neurosurvival. Moreover, activation of this receptor alters the phenotype of immune cells to an anti-inflammatory one. Although cytokines that activate the leukemia inhibitory factor receptor have been studied in the context of neurodegenerative disease, therapeutic targeting of the specific receptor subunit has been understudied in by comparison...
August 18, 2017: Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28822229/vasculogenesis-and-angiogenesis-initiation-under-normoxic-conditions-through-wnt-%C3%AE-catenin-pathway-in-gliomas
#14
Alexandre Vallée, Rémy Guillevin, Jean-Noël Vallée
The canonical Wnt/β-catenin pathway is up-regulated in gliomas and involved in proliferation, invasion, apoptosis, vasculogenesis and angiogenesis. Nuclear β-catenin accumulation correlates with malignancy. Hypoxia activates hypoxia-inducible factor (HIF)-1α by inhibiting HIF-1α prolyl hydroxylation, which promotes glycolytic energy metabolism, vasculogenesis and angiogenesis, whereas HIF-1α is degraded by the HIF prolyl hydroxylase under normoxic conditions. We focus this review on the links between the activated Wnt/β-catenin pathway and the mechanisms underlying vasculogenesis and angiogenesis through HIF-1α under normoxic conditions in gliomas...
August 19, 2017: Reviews in the Neurosciences
https://www.readbyqxmd.com/read/28816340/coagulation-factor-xii-regulates-inflammatory-responses-in-human-lungs
#15
Rosanna Hess, Lukasz Wujak, Christina Hesse, Katherina Sewald, Danny Jonigk, Gregor Warnecke, Hans-Gerd Fieguth, Steven de Maat, Coen Maas, Francesco Bonella, Klaus T Preissner, Benjamin Weiss, Liliana Schaefer, Wolfgang M Kuebler, Philipp Markart, Malgorzata Wygrecka
Increased procoagulant activity in the alveolar compartment and uncontrolled inflammation are hallmarks of the acute respiratory distress syndrome (ARDS). Here, we investigated whether the contact phase system of coagulation is activated and may regulate inflammatory responses in human lungs. Components of the contact phase system were characterized in bronchoalveolar lavage fluids (BALF) from 54 ARDS patients and 43 controls, and their impact on cytokine/chemokine expression in human precision cut lung slices (PCLS) was assessed by a PCR array...
August 17, 2017: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/28808483/therapeutic-immune-monitoring-of-cd4-cd25-t-cells-in-chronic-myeloid-leukemia-patients-treated-with-tyrosine-kinase-inhibitors
#16
Ziyuan Lu, Na Xu, Xuan Zhou, Guanlun Gao, Lin Li, Jixian Huang, Yuling Li, Qisi Lu, Bolin He, Chengyun Pan, Xiaoli Liu
Tyrosine kinase inhibitors (TKIs), including imatinib, dasatinib and nilotinib, are effective forms of therapy for various types of solid cancers and Philadelphia chromosome-positive (Ph(+)) chronic myeloid leukemia. A number of TKIs have been known to have strong effects on T cells, particularly cluster of differentiation (CD) 4(+)CD25(+) T cells, also known as regulatory T cells (Tregs). There is currently a deficit in the available clinical data regarding this area of study. In the present study, a total of 108 peripheral blood samples were collected from patients with chronic myeloid leukemia (CML) at diagnosis (n=31), and at 3 and 6 months following treatment with TKI [imatinib (n=12), dasatinib (n=11) and nilotinib groups (n=8)] and healthy controls (n=15)...
August 2017: Oncology Letters
https://www.readbyqxmd.com/read/28795083/mechanical-instability-and-titanium-particles-induce-similar-transcriptomic-changes-in-a-rat-model-for-periprosthetic-osteolysis-and-aseptic-loosening
#17
Mehdi Amirhosseini, Göran Andersson, Per Aspenberg, Anna Fahlgren
Wear debris particles released from prosthetic bearing surfaces and mechanical instability of implants are two main causes of periprosthetic osteolysis. While particle-induced loosening has been studied extensively, mechanisms through which mechanical factors lead to implant loosening have been less investigated. This study compares the transcriptional profiles associated with osteolysis in a rat model for aseptic loosening, induced by either mechanical instability or titanium particles. Rats were exposed to mechanical instability or titanium particles...
December 2017: Bone Reports
https://www.readbyqxmd.com/read/28794289/differentiation-of-mesenchymal-stem-cells-towards-nephrogenic-lineage-and-their-enhanced-resistance-to-oxygen-peroxide-induced-oxidative-stress
#18
Asima Tayyeb, Naveed Shahzad, Gibran Ali
INTRODUCTION: Mesenchymal stem cells (MSCs) have been publicized to ameliorate kidney injury both in vitro and in vivo. However, very less is known if MSCs can be differentiated towards renal lineages and their further application potential in kidney injuries. MATERIALS AND METHODS: The present study developed a conditioning system of growth factors fibroblast growth factor 2, transforming growth factor-β2, and leukemia inhibitory factor for in vitro differentiation of MSCs isolated from different sources towards nephrogenic lineage...
July 2017: Iranian Journal of Kidney Diseases
https://www.readbyqxmd.com/read/28780571/the-critical-role-of-uterine-cd31-as-a-post-progesterone-signal-in-early-pregnancy
#19
Seo-Ho Lee, Byung-Ju Kim, Uh-Hyun Kim
CD31 has been shown to play a role in endothelial cell migration and angiogenesis, which are critical to the formation and function of the endometrium and myometrium in uterine development during early pregnancy. However, the role of CD31 in uterine receptivity during blastocyst implantation is poorly understood. The pregnancy rate in CD31-/- female mice mated with CD31+/+ male mice was higher than that observed in CD31+/+ female mice mated with CD31+/+ male mice. During the receptive phase of implantation, uterine glands were more developed in CD31-/- mice than in CD31+/+ mice, and the uterine weights of CD31-/- mice were increased...
August 5, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28755912/examination-of-pathways-involved-in-leukemia-inhibitory-factor-lif-induced-cell-growth-arrest-using-label-free-proteomics-approach
#20
Syed Azmal Ali, Gurjeet Kaur, Jai Kumar Kaushik, Dhruba Malakar, Ashok Kumar Mohanty, Sudarshan Kumar
Leukemia inhibitory factor (LIF) is a multifunctional highly glycosylated protein, synthesized and secreted in various body tissues. Besides the abundance in multiple organs, the molecular mechanism underlying the LIF interactions for cell survival and polarity is poorly understood. In the present study, high-resolution LC-MS/MS based LFQ approach identified 2083 proteins with the overall PSM as 16,032. This proteomics data reviles that LIF promotes the AKT-mTOR signaling pathway. It induces cell growth arrest by an intracellular pathways loop to increase the half-life of the cell...
July 26, 2017: Journal of Proteomics
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