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leukemia inhibitory factor

Hyangju Kang, Youngmin Park, Yongjik Lee, Yun-Joo Yoo, Inhwan Hwang
Plants represent promising systems for producing various recombinant proteins. One key area of focus for improving this technology is developing methods for producing recombinant proteins at high levels. Many methods have been developed to increase the transcript levels of recombinant genes. However, methods for increasing protein production involving steps downstream of transcription, including translation, have not been fully explored. Here, we investigated the effects of N-glycosylation on protein production and provide evidence that N-glycosylation greatly increases the expression levels of ER-targeted recombinant proteins...
March 15, 2018: Scientific Reports
Tom Bongiorno, Jeremy Gura, Priyanka Talwar, Dwight Chambers, Katherine M Young, Dalia Arafat, Gonghao Wang, Emily L Jackson-Holmes, Peng Qiu, Todd C McDevitt, Todd Sulchek
The highly proliferative and pluripotent characteristics of embryonic stem cells engender great promise for tissue engineering and regenerative medicine, but the rapid identification and isolation of target cell phenotypes remains challenging. Therefore, the objectives of this study were to characterize cell mechanics as a function of differentiation and to employ differences in cell stiffness to select population subsets with distinct mechanical, morphological, and biological properties. Biomechanical analysis with atomic force microscopy revealed that embryonic stem cells stiffened within one day of differentiation induced by leukemia inhibitory factor removal, with a lagging but pronounced change from spherical to spindle-shaped cell morphology...
2018: PloS One
Juan M Adrian-Segarra, Natalie Schindler, Praveen Gajawada, Holger Lörchner, Thomas Braun, Jochen Pöling
Oncostatin M (OSM) and leukemia inhibitory factor (LIF) are closely related members of the interleukin-6 (IL-6) cytokine family. Both cytokines share a common origin and structure, and both interact through a specific region, termed binding site III, to activate a dimeric receptor complex formed by glycoprotein 130 (gp130) and LIF receptor (LIFR) in humans. However, only OSM activates the OSM receptor (OSMR)/gp130 complex. The molecular features that enable OSM to specifically activate the OSMR are currently unknown...
March 6, 2018: Journal of Biological Chemistry
Ling Wang, Zongliang Jiang, Delun Huang, Jingyue Duan, Chang Huang, Shannon Sullivan, Kaneha Vali, Yexuan Yin, Ming Zhang, Jill Wegrzyn, Xiuchun Cindy Tian, Young Tang
BACKGROUND: The generation of induced pluripotent stem cells (iPSCs) has underdefined mechanisms. In addition, leukemia inhibitory factor (LIF) activated Janus kinase/signal transducer and activator of transcription 3 (JAK/STAT3) pathway is the master regulator for naïve-state pluripotency achievement and maintenance. However, the regulatory process to attain naïve pluripotent iPSCs is not well understood. RESULTS: We performed transcriptome analysis to dissect the genomic expression during mouse iPSC induction, with or without blocking the JAK/STAT3 activity...
March 6, 2018: BMC Genomics
Amina M Abdul-Aziz, Manar S Shafat, Yu Sun, Christopher R Marlein, Rachel E Piddock, Stephen D Robinson, Dylan R Edwards, Zhigang Zhou, Angela Collins, Kristian M Bowles, Stuart A Rushworth
Approximately 80% of patients diagnosed with acute myeloid leukemia (AML) die as a consequence of failure to eradicate the tumor from the bone marrow microenvironment. We have recently shown that stroma-derived interleukin-8 (IL-8) promotes AML growth and survival in the bone marrow in response to AML-derived macrophage migration inhibitory factor (MIF). In the present study we show that high constitutive expression of MIF in AML blasts in the bone marrow is hypoxia-driven and, through knockdown of MIF, HIF1α and HIF2α, establish that hypoxia supports AML tumor proliferation through HIF1α signaling...
February 28, 2018: Oncogene
Suyatno, Yuka Kitamura, Shuntaro Ikeda, Naojiro Minami, Masayasu Yamada, Hiroshi Imai
Undifferentiated spermatogonia eventually differentiate in the testis to produce haploid sperm. Within this cell population, there is a small number of spermatogonial stem cells (SSCs). SSCs are rare cells in the testis, and their cellular characteristics are poorly understood. Establishment of undifferentiated cell line would provide an indispensable tool for studying their biological nature and spermiogenesis/spermatogenesis in vitro. However, there have been few reports on the long-term culture of undifferentiated spermatogonia in species other than rodents...
January 17, 2018: Molecular Reproduction and Development
Tsung-Hsien Yen, Chia-Ling Hsieh, Tsu-Te Liu, Chih-Sheng Huang, Yen-Chung Chen, Yao-Chen Chuang, Song-Shei Lin, Fei-Ting Hsu
The goal of the present study was to investigate anticancer effect of amentoflavone on glioblastoma cells in vitro. Our results demonstrated that amentoflavone not only significantly reduced cell viability, nuclear factor-ĸappa B (NF-ĸB) activation, and protein expression of cellular Fas-associated protein with death domain-like interleukin 1 beta-converting enzyme inhibitory protein (C-FLIP) and myeloid cell leukemia 1 (MCL1), but significantly triggered cell accumulation at the sub-G1 phase, loss of mitochondrial membrane potential, and expression of active caspase-3 and -8...
March 2018: In Vivo
Yae Ohata, Maiko Tsuchiya, Hideaki Hirai, Satoshi Yamaguchi, Takumi Akashi, Kei Sakamoto, Akira Yamaguchi, Tohru Ikeda, Kou Kayamori
The interaction between cancer cells and the cancer stroma plays a crucial role in tumor progression and metastasis in diverse malignancies, including oral cancer. However, the mechanism underlying this interaction remains incompletely elucidated. Here, to investigate the interaction between oral cancer cells and fibroblasts, which are major cellular components of the tumor stroma, we conducted an in vitro study by using human oral squamous cell carcinoma (OSCC) cell lines and normal human dermal fibroblasts (NHDFs)...
2018: PloS One
Suhui Lou, Yanfang Wang, Zujiang Yu, Kelei Guan, Quancheng Kan
BACKGROUND: Curcumin has been used as an alternative medicine for the treatment of infantile hemangiomas (IHs); however, the mechanism underlying the effectiveness of curcumin in IHs remains largely unclear. METHODS: In this study, we isolated primary human hemangioma endothelial cells (HemECs) from fresh surgical specimens of 3 patients. We treated HemECs by curcumin and investigated the alterations in proliferative and apoptotic signaling pathways with cell counting kit-8, flow cytometry, western blotting, immunofluorescence, and real-time polymerase chain reaction...
February 2018: Medicine (Baltimore)
Chihiro Udagawa, Yasushi Sasaki, Hiroshi Suemizu, Yasuyuki Ohnishi, Hiroshi Ohnishi, Takashi Tokino, Hitoshi Zembutsu
Although there has been progress moving from a 'one-size-fits-all' cytotoxic approach to personalized molecular medicine, the majority of patients with cancer receive chemotherapy using cytotoxic anticancer drugs. The sequencing analysis of 409 genes associated with cancer was conducted in the present study using 59 DNA sequences extracted from human cancer xenografts implanted into nude mice, of which sensitivity to 9 cytotoxic anticancer drugs [5-fluorouracil, nimustine, adriamycin, cyclophosphamide, cisplatin, mitomycin C (MMC), methotrexate, vincristine (VCR), and vinblastine] was examined...
February 2018: Experimental and Therapeutic Medicine
Pei-Yuan Zhao, Yong-Qiang Wang, Xi-Hong Liu, Ying-Jun Zhu, Hui Zhao, Qiu-Xia Zhang, Fang Qi, Jun-Ling Li, Nan Zhang, Yong-Ping Fan, Kang-Ning Li, Yuan-Yuan Zhao, Jian-Feng Lei, Lei Wang
ETHNOPHARMACOLOGICAL RELEVANCE: Bu Shen Yi Sui capsule (BSYSC), based on traditional Chinese formula Liu Wei Di Huang pill, is effective for the treatment of multiple sclerosis (MS) in clinical experience and trials. Our previous studies confirmed that BSYSC had the neuroprotective effect in MS and its animal model, experimental autoimmune encephalomyelitis (EAE); however, its mechanism of action was not clear. Thus, the effect of BSYSC on remyelination and the underlying mechanisms were investigated in the EAE mice...
February 8, 2018: Journal of Ethnopharmacology
Li Chen, Ning Hu, Chao Wang, Hong-Mian Zhao
OBJECTIVE: To explore the effect of hypoxia on the cell proliferation and expression of hypoxia-inducible factor-1α(HIF-1α) of human leukemia K562 cells. METHODS: The leukemia cells were divided into 2 groups: hypoxia-treated group and conventional oxygen group as control. The K562 cells in hypoxia-treated group were treated with 50, 200, 400 and 800 µmol/L of CoCl2, while the K562 cells in control group were cultured in conventional oxygen condition, then the K562 cells in 2 groups were colleted after treatment for 24, 48 and 72 hours...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Anna Coulibaly, Astrid Haas, Simone Steinmann, Anke Jakobs, Thomas J Schmidt, Karl-Heinz Klempnauer
Myb is a key regulator of hematopoietic progenitor cell proliferation and differentiation and has emerged as a potential target for the treatment of acute leukemia. Using a myeloid cell line with a stably integrated Myb-inducible reporter gene as a screening tool we have previously identified Celastrol, a natural compound with anti-tumor activity, as a potent Myb inhibitor that disrupts the interaction of Myb with the co-activator p300. We showed that Celastrol inhibits the proliferation of acute myeloid leukemia (AML) cells and prolongs the survival of mice in an in vivo model of AML, demonstrating that targeting Myb with a small-molecule inhibitor is feasible and might have potential as a therapeutic approach against AML...
2018: PloS One
Maryam Farzaneh, Masoumeh Zare, Seyedeh-Nafiseh Hassani, Hossein Baharvand
Pluripotent stem cell (PSC) lines derived from embryonated avian eggs are a convenient platform for production of various recombinant proteins and vaccines. In chicks, both embryonic stem cells (ESC) and embryonic germ cells (EGC) are considered to be pluripotent cells obtained from early blastodermal cells (stage X) and gonadal tissues (stage HH28), respectively. However, the establishment and long-term maintenance of avian PSC lines faces several challenges and differs in efficiency between chick strains...
February 2, 2018: Journal of Cellular Biochemistry
Thomas Pannicke, Lysann Wagner, Andreas Reichenbach, Antje Grosche
Leukemia inhibitory factor (LIF) is a cytokine that exerts different effects in the nervous system. It is involved in neuronal injuries and diseases and is assumed to be neuroprotective and to regulate reactive gliosis. In LIF-deficient (LIF-/-) mice, expression of glial fibrillary acidic protein in retinal Müller glial cells as a hallmark of reactive gliosis is suppressed during retinal degenerations. Here, we detected expression of LIF and its receptors in Müller cells of the murine retina. Moreover, electrophysiological alterations of Müller cells 7 days after transient retinal ischemia were studied by the patch-clamp technique...
January 29, 2018: Neuroscience Letters
Reza Ranjbar, Ansar Karimian, Masoud Aghai, Mehdi Tourani, Seyed Mostafa Mir, Javid Sabour, Farhad Jadidi, Bahman Yousefi
Acute lymphoblastic leukemia (ALL), one of the most common malignant human disorders, originates in different important genetic lesions in T-cell or B-cell progenitors. ALL is a malignant lymphoid progenitor with peak prevalence in children (2-5 years). The rate of survival when one is suffering from ALL depends on various agents including the age of the patient, responses to anti-leukemic therapy, and cell biology. miRNAs and epigenetics are important regulatory factors in the expression of genes. miRNAs are noncoding RNA with inhibitory effectors on specific mRNA...
January 31, 2018: Journal of Cellular Physiology
Ehab Mohamed Gedawy
Novel 4-substitutedaminothieno[2,3-d]pyrimidine derivatives 4a-i and 5a-c were synthesized via reacting corresponding 4-chlorothieno[2,3-d]pyrimidine derivatives 3a-c with N-methylpiperazine, morpholine, N-phenylpiperazine or 1,3-propanediamine. All of the newly synthesized compounds were submitted to the National Cancer Institute (USA) for anticancer evaluation. Six compounds (2a, 4d, 4e, 5a-c) were selected and evaluated using 60 different human tumor cell lines using a single dose (10-5 Molar). Compound 4d showed significant cytotoxicity against colon, renal, prostate and breast cancer...
January 24, 2018: Anti-cancer Agents in Medicinal Chemistry
Hua Li, Juilei Yao, Xingwen Chang, Jinting Wu, Tao Duan, Kai Wang
Preeclampsia (PE) is a pregnancy-specific disorder that is the main cause of maternal and perinatal morbidity and mortality worldwide. Inadequate trophoblastic invasion and endothelial dysfunction in the placenta are considered the foundation of the pathogenesis of preeclampsia in which soluble endoglin (sEng) plays an antiangiogenic role in the development of PE. The leukemia inhibitory factor receptor (LIFR) has been widely studied and is highly involved in arterial injury in vivo and in the migration of cancer cells in vitro...
January 23, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
Yiran Chen, Xiaoling Xie, Anqin Wu, Lei Wang, Yuxing Hu, Honghao Zhang, Yuhua Li
BACKGROUND: Oncogenic roles of epidermal growth factor receptor pathway substrate no.8 (EPS8) have been widely reported in various tumors, making targeting of EPS8 an appealing prospect. Here, we describe the role of EPS8 in acute myeloid leukemia (AML) and consider the potential of EPS8 as an anti-AML target. Nuclear localization signal (NLS) residues of tumor-associated proteins are crucial for cell cycle progression, and specific inhibitors derived from the NLS have inhibitory effect on cancer cells...
January 22, 2018: Journal of Experimental & Clinical Cancer Research: CR
Hongjun Jin, Liyuan Yang, Lu Wang, Zailin Yang, Qian Zhan, Yao Tao, Qin Zou, Yuting Tang, Jingrong Xian, Shuaishuai Zhang, Yipei Jing, Ling Zhang
BACKGROUND: Acute myeloid leukemia (AML) with mutated nucleophosmin (NPM1) has been recognized as a distinct leukemia entity in the 2016 World Health Organization (WHO) classification. The genetic events underlying oncogenesis in NPM1-mutated AML that is characterized by a normal karyotype remain unclear. Inositol polyphosphate 4-phosphatase type II (INPP4B), a new factor in the phosphoinositide-3 kinase (PI3K) pathway-associated cancers, has been recently found a clinically relevant role in AML...
January 17, 2018: Journal of Experimental & Clinical Cancer Research: CR
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