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https://www.readbyqxmd.com/read/28932883/helicase-dependent-isothermal-amplification-a-novel-tool-in-the-development-of-molecular-based-analytical-systems-for-rapid-pathogen-detection
#1
REVIEW
Susana Barreda-García, Rebeca Miranda-Castro, Noemí de-Los-Santos-Álvarez, Arturo J Miranda-Ordieres, María Jesús Lobo-Castañón
Highly sensitive testing of nucleic acids is essential to improve the detection of pathogens, which pose a major threat for public health worldwide. Currently available molecular assays, mainly based on PCR, have a limited utility in point-of-need control or resource-limited settings. Consequently, there is a strong interest in developing cost-effective, robust, and portable platforms for early detection of these harmful microorganisms. Since its description in 2004, isothermal helicase-dependent amplification (HDA) has been successfully applied in the development of novel molecular-based technologies for rapid, sensitive, and selective detection of viruses and bacteria...
September 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28927935/different-effect-of-percutaneous-plate-insertion-via-anteromedial-vs-anterolateral-approach-on-intracompartmental-pressure-of-the-leg-a-cadaveric-study
#2
Chavarin Amarase, Wanchat Thimasarn, Saran Tantavisut, Thanasil Huanmanop, Yongsak Wangroongsub, Worawat Limthongkul
BACKGROUND: Currently Minimally Invasive Plate Osteosynthesis (MIPO) technique for tibial shaft fracture management has gained wide attention. However, an increased intracompartmental pressure after the plate insertion may result in postoperative acute compartment syndrome. We reported the difference of immediate effect of percutaneous plate insertion using 2 approaches of MIPO technique on anterior compartment pressure of the legs. MATERIALS AND METHODS: Eight soft cadaveric legs (one female and three males) without previous history of skeletal trauma or surgery were infused with normal saline to create the sustained intracompartmental pressure of 20mm Hg in all four compartments...
September 6, 2017: Injury
https://www.readbyqxmd.com/read/28918110/emergent-behaviors-in-rbcs-flows-in-micro-channels-using-digital-particle-image-velocimetry
#3
F Cairone, D Ortiz, P J Cabrales, M Intaglietta, M Bucolo
The key points in the design of microfluidic Lab-On-a-Chips for blood tests are the simplicity of the microfluidic chip geometry, the portability of the monitoring system and the ease on-chip integration of the data analysis procedure. The majority of those, recently designed, have been used for blood separation, however their introduction, also, for pathological conditions diagnosis would be important in different biomedical contexts. To overcome this lack is necessary to establish the relation between the RBCs flow and blood viscosity changes in micro-vessels...
September 13, 2017: Microvascular Research
https://www.readbyqxmd.com/read/28917795/portable-near-infrared-spectroscopy-applied-to-quality-control-of-brazilian-coffee
#4
Radigya M Correia, Flavia Tosato, Eloilson Domingos, Rayza R T Rodrigues, Luiz Felipe M Aquino, Paulo R Filgueiras, Valdemar Lacerda, Wanderson Romão
The use of portable micro-spectrometers such as a micro near infrared region (microNIR) spectrometer is a promising technique for solving analytical problems in several areas of science. This work evaluated the potential of microNIR in quality control of Arabica coffee. Arabica coffee has a high commercial value product, motivating the development of analytical methods with high sensitivity and accuracy for detection of its adulteration. Herein, microNIR was successfully used to determine the quality of Arabica coffee by identification and quantification of adulterations such as Robusta coffee (in different roasting levels), as well as corn, peels, and sticks...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917688/a-randomized-trial-of-pocket-echocardiography-integrated-mobile-health-device-assessments-in-modern-structural-heart-disease-clinics
#5
Sanjeev P Bhavnani, Srikanth Sola, David Adams, Ashwin Venkateshvaran, P K Dash, Partho P Sengupta
OBJECTIVES: This study sought to determine whether mobile health (mHealth) device assessments used as clinical decision support tools at the point-of-care can reduce the time to treatment and improve long-term outcomes among patients with rheumatic and structural heart diseases (SHD). BACKGROUND: Newly developed smartphone-connected mHealth devices represent promising methods to diagnose common diseases in resource-limited areas; however, the impact of technology-based care on long-term outcomes has not been rigorously evaluated...
September 9, 2017: JACC. Cardiovascular Imaging
https://www.readbyqxmd.com/read/28915947/immunosensor-array-platforms-based-on-self-assembled-dithiols-for-the-electrochemical-detection-of-tetrodotoxins-in-puffer-fish
#6
Laia Reverté, Katrina Campbell, Maria Rambla-Alegre, Christopher T Elliott, Jorge Diogène, Mònica Campàs
The recent detection of tetrodotoxins (TTXs) in European fish and shellfish has emphasized the urgent need to develop specific, selective, rapid and easy-to-use methods for their detection to assess the potential risk posed to human health. For this purpose, a dithiol self-assembled monolayer (SAM)-based immunoassay previously performed on maleimide plates (mELISA) has been adapted to gold electrode arrays for the development of an electrochemical immunosensor for TTX. The electrochemical SAM-based immunosensor designed herein, provided an oriented, stable and spaced sensing platform for the determination of TTX, attaining a limit of detection of 2...
October 9, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28915277/a-portable-bioelectronic-sensing-system-bessy-for-environmental-deployment-incorporating-differential-microbial-sensing-in-miniaturized-reactors
#7
Alyssa Y Zhou, Moshe Baruch, Caroline M Ajo-Franklin, Michel M Maharbiz
Current technologies are lacking in the area of deployable, in situ monitoring of complex chemicals in environmental applications. Microorganisms metabolize various chemical compounds and can be engineered to be analyte-specific making them naturally suited for robust chemical sensing. However, current electrochemical microbial biosensors use large and expensive electrochemistry equipment not suitable for on-site, real-time environmental analysis. Here we demonstrate a miniaturized, autonomous bioelectronic sensing system (BESSY) suitable for deployment for instantaneous and continuous sensing applications...
2017: PloS One
https://www.readbyqxmd.com/read/28914453/potential-effectiveness-of-visible-and-near-infrared-spectroscopy-coupled-with-wavelengths-selection-for-real-time-grapevine-leaf-water-status-measurement
#8
Valentina Giovenzana, Roberto Beghi, Simone Parisi, Lucio Brancadoro, Riccardo Guidetti
BACKGROUND: Increasing attention is paid to non-destructive methods for water status real time monitoring as a potential solution, replacing the tedious conventional techniques which are time consuming and not easily to perform directly in field. The objective was to test the potential effectiveness of two portable optical devices (visible/near infrared, vis/NIR; and near infrared, NIR) for a rapid and non-destructive evaluation of the water status of grapevine leaves. Moreover, a variable selection methodology was proposed to determine a set of candidate variables for the prediction of water potential (Ψ; MPa) related to leaf water status in a view of a simplified optical device...
September 15, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28903044/engineering-synthetic-signaling-pathways-with-programmable-dcas9-based-chimeric-receptors
#9
Toni A Baeumler, Ahmed Ashour Ahmed, Tudor A Fulga
Synthetic receptors provide a powerful experimental tool for generation of designer cells capable of monitoring the environment, sensing specific input signals, and executing diverse custom response programs. To advance the promise of cellular engineering, we have developed a class of chimeric receptors that integrate a highly programmable and portable nuclease-deficient CRISPR/Cas9 (dCas9) signal transduction module. We demonstrate that the core dCas9 synthetic receptor (dCas9-synR) architecture can be readily adapted to various classes of native ectodomain scaffolds, linking their natural inputs with orthogonal output functions...
September 12, 2017: Cell Reports
https://www.readbyqxmd.com/read/28902149/highly-portable-sensor-based-system-for-human-fall-monitoring
#10
Aihua Mao, Xuedong Ma, Yinan He, Jie Luo
Falls are a very dangerous situation especially among elderly people, because they may lead to fractures, concussion, and other injuries. Without timely rescue, falls may even endanger their lives. The existing optical sensor-based fall monitoring systems have some disadvantages, such as limited monitoring range and inconvenience to carry for users. Furthermore, the fall detection system based only on an accelerometer often mistakenly determines some activities of daily living (ADL) as falls, leading to low accuracy in fall detection...
September 13, 2017: Sensors
https://www.readbyqxmd.com/read/28901736/facile-fabrication-of-flexible-linbo3-piezoelectric-sensor-through-hot-pressing-for-biomechanical-monitoring
#11
Muzhen Xu, Hua Kang, Li Guan, Huayi Li, Meining Zhang
Wearable pressure sensors due to their portability and flexibility, have been attracting increasing attention for biomechanical monitoring. However, although great advances have been made by many efforts, the sensors with good performance produced by facile methods are insufficient. Here, we present a simple method for simple manufacturing one flexible and self-powered piezoelectric sensor based on LiNbO3 (LN) particles. The LN particles are dispersed in polypropylene (PP) doped with multi-walled carbon nanotubes (MWCNTs) through hot-pressing (200°C), forming LN/MWCNTs/PP piezoelectric composite film (PCF) flexible sensor...
September 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28895397/screen-printed-polyaniline-based-electrodes-for-the-real-time-monitoring-of-lamp-reactions
#12
David Gosselin, Maxime Gougis, Mélissa Baque, Fabrice P Navarro, Mohamed Naceur Belgacem, Didier Chaussy, Anne-Gaelle Bourdat, Pascal Mailley, Jean Berthier
Nucleic acid amplification testing is a very powerful method to perform efficient and early diagnostics. However, the integration of a DNA amplification reaction with its associ-ated detection in a low-cost, portable and autonomous device remains challenging. Addressing this challenge, the use of screen-printed electrochemical sensor is reported. To achieve the detection of the DNA amplification reaction, a real-time monitoring of the hydronium ions concentration, a by-product of this reaction, is performed...
September 12, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28895272/stretchable-electronic-sensors-of-nanocomposite-network-films-for-ultrasensitive-chemical-vapor-sensing
#13
Hong Yan, Mengjuan Zhong, Ze Lv, Pengbo Wan
A stretchable, transparent, and body-attachable chemical sensor is assembled from the stretchable nanocomposite network film for ultrasensitive chemical vapor sensing. The stretchable nanocomposite network film is fabricated by in situ preparation of polyaniline/MoS2 (PANI/MoS2 ) nanocomposite in MoS2 suspension and simultaneously nanocomposite deposition onto prestrain elastomeric polydimethylsiloxane substrate. The assembled stretchable electronic sensor demonstrates ultrasensitive sensing performance as low as 50 ppb, robust sensing stability, and reliable stretchability for high-performance chemical vapor sensing...
September 12, 2017: Small
https://www.readbyqxmd.com/read/28893111/using-tablet-based-technology-to-deliver-time-efficient-ototoxicity-monitoring
#14
Douglas Brungart, Jaclyn Schurman, Dawn Konrad-Martin, Kelly Watts, Jay Buckey, Odile Clavier, Peter G Jacobs, Samuel Gordon, Marilyn F Dille
OBJECTIVE: The goal of this article is to highlight mobile technology that is not yet standard of care but could be considered for use in an ototoxicity monitoring programme (OMP) as an adjunct to traditional audiometric testing. Current guidelines for ototoxicity monitoring include extensive test protocols performed by an audiologist in an audiometric booth. This approach is comprehensive, but it may be taxing for patients suffering from life-threatening illnesses and cost prohibitive if it requires serial clinical appointments...
September 12, 2017: International Journal of Audiology
https://www.readbyqxmd.com/read/28884162/modifier-free-microfluidic-electrochemical-sensor-for-heavy-metal-detection
#15
Liu-Liu Shen, Gui-Rong Zhang, Wei Li, Markus Biesalski, Bastian J M Etzold
Heavy-metal pollution poses severe threat to ecological systems and presents a great challenge for global sustainability. Portable point-of-care sensing platform for detection/monitoring of heavy-metal pollution in the environment is urgently demanded. Herein, a highly sensitive, robust, and low-cost microfluidic electrochemical carbon-based sensor (μCS) for detection of trace heavy metals is presented. The miniaturized μCS devices are based on a microfluidic paper channel combined with a novel three-dimensional layout with working and counter electrodes facing each other and analyte flowing along the microfluidic channel between these two electrodes...
August 31, 2017: ACS Omega
https://www.readbyqxmd.com/read/28882019/usefulness-of-hospital-at-home-in-nosocomial-infections-advantages-and-limitations
#16
REVIEW
V J González Ramallo, M Mirón Rubio, O Estrada Cuxart, M E García Leoni
Hospital at Home units allows ambulatory treatment and monitoring of complex and serious infections. Nosocomial infections produce an extension of the stay in hospital often specifying long intravenous treatments without any effective oral alternatives. Daily dosing of antimicrobial are easier to administer at home. The use of portable programmable pump infusion and elastomeric devices allow efficient and safe infu-sions for most antimicrobials at home. Some antibiotics against multidrug-resistant organisms of recent introduction have a suitable profile for outpatient intravenous treatment...
September 2017: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/28880397/transcranial-doppler-a-stethoscope-for-the-brain-neurocritical-care-use
#17
REVIEW
Chiara Robba, Danilo Cardim, Mypinder Sekhon, Karol Budohoski, Marek Czosnyka
Transcranial Doppler (TCD) ultrasonography is a noninvasive bedside monitoring technique that can evaluate cerebral blood flow hemodynamics in the intracranial arterial vasculature. TCD allows assessment of linear cerebral blood flow velocity, with a high temporal resolution and is inexpensive, reproducible, and portable. The aim of this review is to provide an overview of the most commonly used TCD derived signals and measurements used commonly in neurocritical care. We describe both basic (flow velocity, pulsatility index) and advanced concepts, including critical closing pressure, wall tension, autoregulation, noninvasive intracranial pressure, brain compliance, and cerebrovascular time constant; we also describe the clinical applications of TCD to highlight their utility in the diagnosis and monitoring of cerebrovascular diseases as the "stethoscope for the brain...
September 7, 2017: Journal of Neuroscience Research
https://www.readbyqxmd.com/read/28871304/an-electrochemiluminescence-cloth-based-biosensor-with-smartphone-based-imaging-for-detection-of-lactate-in-saliva
#18
Yong Yao, Huijie Li, Dan Wang, Cuiling Liu, Chunsun Zhang
Cloth fabrics and smartphones have become the two things people are most familiar with. Particularly, smartphones are used as portable personal computers, revolutionizing communication and lifestyle. Here, screen-printing technology is applied to fabricate carbon electrodes and electrochemical chambers on a single hydrophilic cloth, while the cloth-based electrochemiluminescence (ECL) signals are read out by using an inexpensive smartphone. Therefore, the ECL detection is available in both low-cost disposable sensors and a portable format, which may be very suitable to be used as a non-invasive monitoring tool for medical diagnostics...
September 5, 2017: Analyst
https://www.readbyqxmd.com/read/28865923/rapid-and-label-free-electrochemical-dna-biosensor-for-detecting-hepatitis-a-virus
#19
Marisa Manzano, Sara Viezzi, Sandra Mazerat, Robert S Marks, Jasmina Vidic
Diagnostic systems that can deliver highly specific and sensitive detection of hepatitis A virus (HAV) in food and water are of particular interest in many fields including food safety, biosecurity and control of outbreaks. Our aim was the development of an electrochemical method based on DNA hybridization to detect HAV. A ssDNA probe specific for HAV (capture probe) was designed and tested on DNAs from various viral and bacterial samples using Nested-Reverse Transcription Polymerase Chain Reaction (nRT-PCR)...
August 19, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28865401/spatial-and-temporal-determinants-of-a-weighted-and-frequency-specific-sound-levels-an-elastic-net-approach
#20
Erica D Walker, Jaime E Hart, Petros Koutrakis, Jennifer M Cavallari, Trang VoPham, Marcos Luna, Francine Laden
BACKGROUND: Urban sound levels are a ubiquitous environmental stressor and have been shown to be associated with a wide variety of health outcomes. While much is known about the predictors of A-weighted sound pressure levels in the urban environment, far less is known about other frequencies. OBJECTIVE: To develop a series of spatial-temporal sound models to predict A-weighted sound pressure levels, low, mid, and high frequency sound for Boston, Massachusetts. METHODS: Short-term sound levels were gathered at n = 400 sites from February 2015 - February 2016...
August 30, 2017: Environmental Research
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