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https://www.readbyqxmd.com/read/29231595/-effects-of-electroacupuncture-on-activation-of-microglia-cells-in-spinal-cord-in-rats-with-neuropathic-pain
#1
Sisi Li, Pengpeng Gu, Wenzhan Tu, Xia Jiang, Wenci Chen, Qimiao Hu, Songhe Jiang
OBJECTIVE: To observe the effects of electroacupuncture (EA) on the activation of microglia cells in the L4 to L6 spinal cord in rats with neuropathic pain, so as to investigate whether EA could inhibit the activation of spinal microglial cells and regulate the expression of brain-derived neurotrophic factor (BDNF) to achieve the analgesic effect. METHODS: Forty male Sprague Dawley rats were randomly divided into a normal group, a sham-model group, a model group and an EA group, 10 rats in each one...
April 12, 2017: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
https://www.readbyqxmd.com/read/29223561/microglia-and-astrocyte-activation-in-the-spinal-cord-of-lame-horses
#2
Constanza S Meneses, Heine Y Müller, Daniel E Herzberg, Benjamin Uberti, Marianne P Werner, Hedie A Bustamante
OBJECTIVE: To determine the microglial and astrocyte response to painful lameness in horses. STUDY DESIGN: Ionized calcium binding adaptor molecule 1 (Iba-1) and glial fibrillary acidic protein (GFAP) expression, cell density and morphology were determined through immunofluorescence within the dorsal horn of equine spinal cord. ANIMALS: A total of five adult horses with acute or chronic unilateral lameness, previously scheduled for euthanasia...
November 2, 2017: Veterinary Anaesthesia and Analgesia
https://www.readbyqxmd.com/read/29222781/cell-enumeration-assays-application-of-the-mtt-and-sulforhodamine-b-assays-to-lipopolysaccharide-stimulated-neonatal-rodent-microglia
#3
Laura Facci, Stephen D Skaper
Glial cell activation, in particular microglia, plays an important role in the pathogenesis of various neurodegenerative disorders as well as in chronic and neuropathic pain. This chapter compares two established cell enumeration assays, namely, the colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay and the protein-binding sulforhodamine B assay for microglia as a function of culture condition and activation state. The pros and cons of each are then described.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29222778/astrocyte-microglia-cocultures-as-a-model-to-study-neuroinflammation
#4
Laura Facci, Massimo Barbierato, Stephen D Skaper
Glial cell activation plays an important role in the pathogenesis of various neurodegenerative disorders as well as in chronic and neuropathic pain. This chapter describes a model which allows one to assess the individual and combined contributions of astrocytes and microglia in response to a pro-inflammatory stimulus, with emphasis on ionotropic purinergic receptors.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29201256/neuropeptides-and-atp-signaling-in-the-trigeminal-ganglion
#5
REVIEW
Tetsuya Goto, Haruki Iwai, Eriko Kuramoto, Atsushi Yamanaka
Peripheral nociceptive stimuli from orofacial structures are largely transmitted by the trigeminal nerve. According to the peripheral noxious stimuli, neurons in the trigeminal ganglion (TG) produce neuropeptides such as substance P, and calcitonin-gene-related peptide, etc. Beside the production of neuropeptides, there exists unique non-synaptic interaction system between maxillary and mandibular neurons in the TG. Neurons in the TG are surrounded by satellite glial cells (SGCs), which initially receive the signal from TG neurons...
November 2017: Japanese Dental Science Review
https://www.readbyqxmd.com/read/29196874/upregulation-of-spinal-voltage-dependent-anion-channel-1-contributes-to-bone-cancer-pain-hypersensitivity-in-rats
#6
Xiangpeng Kong, Jinrong Wei, Diyu Wang, Xiaoju Zhu, Youlang Zhou, Shusheng Wang, Guang-Yin Xu, Guo-Qin Jiang
Voltage-dependent anion channel 1 (VDAC1) is thought to contribute to the progression of tumor development. However, whether VDAC1 contributes to bone cancer pain remains unknown. In this study, we found that the expression of VDAC1 was upregulated in the L2-5 segments of the spinal dorsal horn at 2 and 3 weeks after injection of tumor cells into the tibial cavity. Intrathecal injection of a VDAC1 inhibitor significantly reversed the pain hypersensitivity and reduced the over-expression of Toll-like receptor 4 (TLR4)...
December 1, 2017: Neuroscience Bulletin
https://www.readbyqxmd.com/read/29179879/p2x4-a-fast-and-sensitive-purinergic-receptor
#7
REVIEW
Jaanus Suurväli, Pierre Boudinot, Jean Kanellopoulos, Sirje Rüütel Boudinot
Extracellular nucleotides have been recognized as important mediators of activation, triggering multiple responses via plasma membrane receptors known as P2 receptors. P2 receptors comprise P2X ionotropic receptors and G protein-coupled P2Y receptors. P2X receptors are expressed in many tissues, where they are involved in a number of functions including synaptic transmission, muscle contraction, platelet aggregation, inflammation, macrophage activation, differentiation and proliferation, neuropathic and inflammatory pain...
October 2017: Biomedical Journal
https://www.readbyqxmd.com/read/29149212/mycolactone-displays-anti-inflammatory-effects-on-the-nervous-system
#8
Caroline Isaac, Annie Mauborgne, Alfonso Grimaldi, Kemy Ade, Michel Pohl, Cristina Limatola, Yves Boucher, Caroline Demangel, Laure Guenin-Macé
BACKGROUND: Mycolactone is a macrolide produced by the skin pathogen Mycobacterium ulcerans, with cytotoxic, analgesic and immunomodulatory properties. The latter were recently shown to result from mycolactone blocking the Sec61-dependent production of pro-inflammatory mediators by immune cells. Here we investigated whether mycolactone similarly affects the inflammatory responses of the nervous cell subsets involved in pain perception, transmission and maintenance. We also investigated the effects of mycolactone on the neuroinflammation that is associated with chronic pain in vivo...
November 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29140922/fingolimod-reduces-neuropathic-pain-behaviors-in-a-mouse-model-of-multiple-sclerosis-by-a-sphingosine-1-phosphate-receptor-1-dependent-inhibition-of-central-sensitization-in-the-dorsal-horn
#9
Suzanne Doolen, Tommaso Iannitti, Benjamin C Shaw, Carolyn M Grachen, Renee R Donahue, Bradley K Taylor
Multiple sclerosis (MS) is an autoimmune-inflammatory neurodegenerative disease that is often accompanied by a debilitating neuropathic pain. Disease-modifying agents slow the progression of MS and prevent relapses, yet it remains unclear if they yield analgesia. We explored the analgesic potential of fingolimod (FTY720), an agonist/functional antagonist at the sphingosine-1-phosphate receptor 1 (S1PR1), because it reduces hyperalgesia in models of peripheral inflammatory and neuropathic pain. We used a myelin oligodendrocyte glycoprotein 35-55 (MOG35-55) mouse model of experimental autoimmune encephalomyelitis (EAE), modified to avoid frank paralysis and thus allow for assessment of withdrawal behaviors to somatosensory stimuli...
November 13, 2017: Pain
https://www.readbyqxmd.com/read/29135586/increased-hyperalgesia-and-proinflammatory-cytokines-in-the-spinal-cord-and-dorsal-root-ganglion-after-surgery-and-or-fentanyl-administration-in-rats
#10
Lu Chang, Fang Ye, Quehua Luo, Yuanxiang Tao, Haihua Shu
BACKGROUND: Perioperative fentanyl has been reported to induce hyperalgesia and increase postoperative pain. In this study, we tried to investigate behavioral hyperalgesia, the expression of proinflammatory cytokines, such as interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), and the activation of microglia in the spinal cord and dorsal root ganglion (DRG) in a rat model of surgical plantar incision with or without perioperative fentanyl. METHODS: Four groups of rats (n = 32 for each group) were subcutaneously injected with fentanyl at 60 μg/kg or normal saline for 4 times with 15-minute intervals...
November 10, 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/29108992/mir-32-5p-mediated-dusp5-downregulation-contributes-to-neuropathic-pain
#11
Tingfei Yan, Fuguo Zhang, Chenxi Sun, Jingchuan Sun, Yuan Wang, Ximing Xu, Jiangang Shi, Guodong Shi
Previous studies have demonstrated that microRNAs (miRNAs) play important roles in the pathogenesis of neuropathic pain. In the present study, we found that miR-32-5p was significantly upregulated in rats after spinal nerve ligation (SNL), specifically in the spinal microglia of rats with SNL. Functional assays showed that knockdown of miR-32-5p greatly suppressed mechanical allodynia and heat hyperalgesia, and decreased inflammatory cytokine (IL-1β, TNF-α and IL-6) protein expression in rats after SNL. Similarly, miR-32-5p knockdown alleviated cytokine production in lipopolysaccharide (LPS)-treated spinal microglial cells, whereas its overexpression had the opposite effect...
November 3, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29107326/adult-hippocampal-neurogenesis-in-neuropathic-pain-and-alkyl-glycerol-ethers-treatment
#12
Anna A Tyrtyshnaia, Igor V Manzhulo, Ruslan M Sultanov, Ekaterina V Ermolenko
Neuropathic pain manifested by a number of sensory symptoms is often accompanied by disorders of higher nervous activity, such as memory impairment, depression, anxiety, anhedonia, etc. This emphasizes the involvement of supraspinal structures including the hippocampus in neuropathic pain pathogenesis. In the present study, we focused on the impact of chronic neuropathic pain on hippocampal neurogenesis and microglial state. In addition, we test the effect of alkyl glycerol ethers on hippocampal neuronal and microglial plasticity as well as behavioral parameters...
October 26, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/29105386/the-effect-of-ethanolic-extract-of-lippia-citriodora-on-rats-with-chronic-constriction-injury-of-neuropathic-pain
#13
Bahareh Amin, Reyhaneh Noorani, Bibi Marjan Razavi, Hossein Hosseinzadeh
OBJECTIVES: We examined the protective effects of ethanolic extract of Lippia citriodora (L. citriodora) on rats subjected to chronic constriction injury (CCI) of sciatic nerve and possible mechanisms of actions. MATERIALS AND METHODS: In this experimental study, the extract was administered 50, 100 and 200 mg/kg, Intraperitoneally (I.P) from the surgery time for 14 consecutive days. The changes in the spinal cord levels of apoptotic factors, microglia and astroglia markers during the time course of study were assessed by western blotting on days 3, 7 and 14 post-CCI...
January 2018: Cell Journal
https://www.readbyqxmd.com/read/29096659/lidocaine-alleviates-morphine-tolerance-via-ampk-socs3-dependent-neuroinflammation-suppression-in-the%C3%A2-spinal-cord
#14
Yan Zhang, Gao-Jian Tao, Liang Hu, Jie Qu, Yuan Han, Guangqin Zhang, Yanning Qian, Chun-Yi Jiang, Wen-Tao Liu
BACKGROUND: Morphine tolerance is a clinical challenge, and its pathogenesis is closely related to the neuroinflammation mediated by Toll-like receptor 4 (TLR4). In Chinese pain clinic, lidocaine is combined with morphine to treat chronic pain. We found that lidocaine sufficiently inhibited neuroinflammation induced by morphine and improved analgesic tolerance on the basis of non-affecting pain threshold. METHODS: CD-1 mice were utilized for tail-flick test to evaluate morphine tolerance...
November 2, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29096654/the-analgesic-effects-of-triptolide-in-the-bone-cancer-pain-rats-via-inhibiting-the-upregulation-of-hdacs-in-spinal-glial-cells
#15
Xiao-Fan Hu, Xiao-Tao He, Kai-Xiang Zhou, Chen Zhang, Wen-Jun Zhao, Ting Zhang, Jin-Lian Li, Jian-Ping Deng, Yu-Lin Dong
BACKGROUND: Bone cancer pain (BCP) severely compromises the quality of life, while current treatments are still unsatisfactory. Here, we tested the antinociceptive effects of triptolide (T10), a substance with considerable anti-tumor efficacies on BCP, and investigated the underlying mechanisms targeting the spinal dorsal horn (SDH). METHODS: Intratibial inoculation of Walker 256 mammary gland carcinoma cells was used to establish a BCP model in rats. T10 was intrathecally injected, and mechanical allodynia was tested by measuring the paw withdrawal thresholds (PWTs)...
November 2, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29096563/preconditioning-with-hydrogen-sulfide-prevents-bone-cancer-pain-in-rats-through-a-proliferator-activated-receptor-gamma-p38-jun-n-terminal-kinase-pathway
#16
Li Zhuang, Ke Li, Gaowei Wang, Tao Shou, Chunlin Gao, Yong Mao, Mingliang Bao, Mingli Zhao
Bone cancer pain (BCP) is a severe type of hyperpathic pain occurring with primary bone tumors or advanced cancers which metastasize to bones. BCP can detrimentally reduce quality of life and presents a challenge to modern medicine. Studies have shown that exogenous H2S may act as a neuroprotectant to protect against some diseases in central nervous system. The preset study aimed to investigate the antinociceptive effect of H2S in BCP. We first measured the changes of serum H2S in patients with BCP and analyzed the relationship between them, then investigated the effect of H2S preconditioning on BCP, and explored the mechanism in rat model...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29081070/paeoniflorin-attenuates-postoperative-pain-by-suppressing-matrix-metalloproteinase-9-2-in-mice
#17
Y-X Fan, L Hu, S-H Zhu, Y Han, W-T Liu, Y-J Yang, Q-P Li
BACKGROUND: Recent studies have implicated that matrix metalloproteinase (MMP)-9 and MMP-2 play key roles in neuropathic pain due to their facilitation of inflammatory cytokine maturation and induction of neuroinflammation. However, the role of MMP-9/2 in postoperative pain is still unclear. We previously suggested that the natural compound paeoniflorin inhibited microglia activation induced by morphine treatment. In the present study, we demonstrated that paeoniflorin could alleviate postoperative pain via specific inhibition of matrix metalloproteinases (MMPs)...
October 29, 2017: European Journal of Pain: EJP
https://www.readbyqxmd.com/read/29080683/interaction-between-astrocytic-colony-stimulating-factor-and-its-receptor-on-microglia-mediates-central-sensitization-and-behavioral-hypersensitivity-in-chronic-post-ischemic-pain-model
#18
Yuying Tang, Lian Liu, Dan Xu, Wensheng Zhang, Yi Zhang, Jieshu Zhou, Wei Huang
Accumulation of microglia occurs in the dorsal horn in the rodent model of chronic post ischemic pain (CPIP), while the mechanism how microglia affects the development of persistent pain largely remains unknown. Here, using a rodent model of CPIP induced by ischemia-reperfusion (IR) injury in the hindpaw, we observed that microglial accumulation occurred in the ipsilateral dorsal horn after ischemia 3h, and in ipsilateral and contralateral dorsal horn in the rats with ischemia 6h. The accumulated microglia released BDNF, increased neuronal excitability in dorsal horn, and produced pain behaviors in the modeled rodents...
October 30, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/29079733/analgesic-effects-and-pharmacologic-mechanisms-of-the-gelsemium-alkaloid-koumine-on-a-rat-model-of-postoperative-pain
#19
Bo-Jun Xiong, Ying Xu, Gui-Lin Jin, Ming Liu, Jian Yang, Chang-Xi Yu
Postoperative pain (POP) of various durations is a common complication of surgical procedures. POP is caused by nerve damage and inflammatory responses that are difficult to treat. The neuroinflammation-glia-steroid network is known to be important in POP. It has been reported that the Gelsemium alkaloid koumine possesses analgesic, anti-inflammatory and neurosteroid modulating activities. This study was undertaken to test the analgesic effects of koumine against POP and explore the underlying pharmacologic mechanisms...
October 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29076792/targeting-il-33-st2-signaling-regulation-of-immune-function-and-analgesia
#20
Victor Fattori, Miriam S N Hohmann, Ana C Rossaneis, Marilia F Manchope, Jose C Alves-Filho, Thiago M Cunha, Fernando Q Cunha, Waldiceu A Verri
IL-33 signals through ST2 receptor and promotes inflammation by activating downstream pathways culminating in the production of pro-inflammatory mediators such as IL-1β, TNF-α, and IL-6 in an NF-κB-dependent manner. In fact, compelling evidence has demonstrated the importance of IL-33/ST2 in both innate and adaptive immune responses in diseases presenting pain as an important clinical symptom. Areas covered: IL-33 is a pleiotropic cytokine with varied immune functions. Dysregulation of this pathway has been described as a key step in varied immune responses...
November 5, 2017: Expert Opinion on Therapeutic Targets
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