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https://www.readbyqxmd.com/read/29457597/injectable-collagen-rgd-systems-for-bone-tissue-engineering-applications
#1
Fu-Chen Kung
Imbalance crosslink density and polymer concentration gradient is formed within the traditional alginate hydrogel using calcium chloride as a crosslinking agent in external gelation for instantaneously process. In this studying, type I collagen (Col I) blended calcium salt form of poly(γ-glutamic acid) (γCaPGA) was mixing with RGD-modified alginate with convenient gelation process and suitable for practical use. The hydrophilicity of the resulting hydrogels was evaluated through swelling tests, water retention capacity tests, and water vapor permeation tests...
2018: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/29454157/nanofibrous-plga-electrospun-scaffolds-modified-with-type-i-collagen-influence-hepatocyte-function-and-support-viability-in-vitro
#2
Jessica H Brown, Prativa Das, Michael D DiVito, David Ivancic, Lay Poh Tan, Jason A Wertheim
A major challenge of maintaining primary hepatocytes in vitro is progressive loss of hepatocyte-specific functions, such as protein synthesis and cytochrome P450 (CYP450) catalytic activity. We developed a three-dimensional (3D) nanofibrous scaffold made from poly(L-lactide-co-glycolide) (PLGA) polymer using a newlyoptimizedwet electrospinning techniquethat resulted in a highly porous structure that accommodated inclusion of primary human hepatocytes. Extracellular matrix (ECM) proteins (type I collagen or fibronectin) at varying concentrations were chemically linked to electrospun PLGA using amine coupling to develop an in vitro culture system containing the minimal essential ECM components of the liver micro-environment that preserve hepatocyte function in vitro...
February 14, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29452948/photo-tearable-tape-close-wrapped-upconversion-nanocapsules-for-near-infrared-modulated-efficient-sirna-delivery-and-therapy
#3
Yue Zhang, Kewei Ren, Xiaobo Zhang, Zhicong Chao, Yuqin Yang, Deju Ye, Zhihui Dai, Ying Liu, Huangxian Ju
RNA interference (RNAi) has become an appealing therapeutic approach for cancer and other diseases. One key challenge is the effective protection of these small fragile biomolecules against complicated physiological environments as well as efficient on-demand release. Here we design a photo-tearable polymer tape close-wrapped nanocapsule for efficient NIR modulated siRNA delivery. The photo-tearable nanocapsules comprise core-shell upconversion nanoparticles (UCNPs) coated with mesoporous silica layer for loading of photosensitizer hypocrellin A (HA) and small interfering RNA (siRNA) against polo-like kinase 1 (PLK1), and covalently bound thin membranes of polyethylene glycol (PEG) via a synthesized photocleavable linker (PhL)...
February 9, 2018: Biomaterials
https://www.readbyqxmd.com/read/29450862/recent-progress-in-dendrimer-based-nanomedicine-development
#4
REVIEW
Yejin Kim, Eun Ji Park, Dong Hee Na
Dendrimers offer well-defined nanoarchitectures with spherical shape, high degree of molecular uniformity, and multiple surface functionalities. Such unique structural properties of dendrimers have created many applications for drug and gene delivery, nanomedicine, diagnostics, and biomedical engineering. Dendrimers are not only capable of delivering drugs or diagnostic agents to desired sites by encapsulating or conjugating them to the periphery, but also have therapeutic efficacy in their own. When compared to traditional polymers for drug delivery, dendrimers have distinct advantages, such as high drug-loading capacity at the surface terminal for conjugation or interior space for encapsulation, size control with well-defined numbers of peripheries, and multivalency for conjugation to drugs, targeting moieties, molecular sensors, and biopolymers...
February 15, 2018: Archives of Pharmacal Research
https://www.readbyqxmd.com/read/29449882/mathematical-models-of-lignin-biosynthesis
#5
Mojdeh Faraji, Luis L Fonseca, Luis Escamilla-Treviño, Jaime Barros-Rios, Nancy Engle, Zamin K Yang, Timothy J Tschaplinski, Richard A Dixon, Eberhard O Voit
Background: Lignin is a natural polymer that is interwoven with cellulose and hemicellulose within plant cell walls. Due to this molecular arrangement, lignin is a major contributor to the recalcitrance of plant materials with respect to the extraction of sugars and their fermentation into ethanol, butanol, and other potential bioenergy crops. The lignin biosynthetic pathway is similar, but not identical in different plant species. It is in each case comprised of a moderate number of enzymatic steps, but its responses to manipulations, such as gene knock-downs, are complicated by the fact that several of the key enzymes are involved in several reaction steps...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29449176/rubber-gloves-biodegradation-by-a-consortium-mixed-culture-and-pure-culture-isolated-from-soil-samples
#6
Chairat Nawong, Kamontam Umsakul, Natthawan Sermwittayawong
An increasing production of natural rubber (NR) products has led to major challenges in waste management. In this study, the degradation of rubber latex gloves in a mineral salt medium (MSM) using a bacterial consortium, a mixed culture of the selected bacteria and a pure culture were studied. The highest 18% weight loss of the rubber gloves were detected after incubated with the mixed culture. The increased viable cell counts over incubation time indicated that cells used rubber gloves as sole carbon source leading to the degradation of the polymer...
February 3, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29446934/guanidinium-functionalized-interpolyelectrolyte-complexes-enable-rnai-in-resistant-insect-pests
#7
Keith H Parsons, Mosharrof H Mondal, Charles L McCormick, Alex S Flynt
RNAi-based technologies are ideal for pest control as they can provide species specificity and spare non-target organisms. However, in some pests biological barriers prevent use of RNAi, and therefore broad application. In this study we tested the ability of a synthetic cationic polymer, poly-[N-(3-guanidinopropyl)methacrylamide] (pGPMA), that mimics arginine-rich cell penetrating peptides to trigger RNAi in an insensitive animal-Spodoptera frugiperda. Polymer-dsRNA interpolyelectrolyte complexes (IPECs) were found to be efficiently taken up by cells, and to drive highly efficient gene knockdown...
February 15, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29446914/simultaneous-detection-of-human-c-terminal-p53-isoforms-by-single-template-molecularly-imprinted-polymers-mips-coupled-with-liquid-chromatography-tandem-mass-spectrometry-lc-ms-ms-based-targeted-proteomics
#8
Wenting Jiang, Liang Liu, Yun Chen
Abnormal expression of C-terminal p53 isoforms α, β, and γ can cause the development of cancers including breast cancer. To date, much evidence has demonstrated that these isoforms can differentially regulate target genes and modulate their expression. Thus, quantification of individual isoforms may help to link clinical outcome to p53 status and to improve cancer patient treatment. However, there are few studies on accurate determination of p53 isoforms, probably due to sequence homology of these isoforms and also their low abundance...
February 15, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29442622/chitosan-stabilized-gold-folate-poly-lactide-co-glycolide-nanoplexes-facilitate-efficient-gene-delivery-in-hepatic-and-breast-cancer-cells
#9
Jude Akinyelu, Moganavelli Singh
The biodegradable polymer, poly(lactide-co-glycolide) is a popular polymer of choice in many nanotherapeutic studies. Herein, we report on the synthesis and evaluation of four chitosan stabilized poly(lactide-co-glycolide) nanoparticles with and without coating with gold, and the targeting ligand, folic acid, as potential non-viral gene delivery vectors. The poly(lactide-co-glycolide) nanoparticles were synthesized via nanoprecipitation/solvent evaporation method in conjunction with the surface functionalizing folic acid and chitosan...
July 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29436180/sweet-vector-for-gene-delivery-the-sugar-decoration-of-polyplexes-reduces-cytotoxicity-with-a-balanced-effect-on-gene-expression
#10
Lindomar J C Albuquerque, Alex C Alavarse, Maria C Carlan da Silva, Morgana S Zilse, Maitê T Barth, Ismael C Bellettini, Fernando C Giacomelli
The use of sugar-functionalized polyplexes as a nonviral gene delivery vector with lower cytotoxicity than the well-known polymeric carrier branched polyethyleneimine (BPEI) is investigated. The substitution of primary amine groups in the BPEI chains with lactose residues leads to larger polyplexes, presumably due to the higher amount of polymer required to complete DNA condensation. Nevertheless, the sugar functionalization substantially reduces the cytotoxicity of the assemblies. The nanocomplexes are taken up by the cells to a greater extent, whereas the levels of gene expression are maintained compared to those obtained using BPEI, which is known for its excellent transfection efficiency...
February 2018: Macromolecular Bioscience
https://www.readbyqxmd.com/read/29433939/targeted-mrna-therapy-for-ornithine-transcarbamylase-deficiency
#11
Mary G Prieve, Pierrot Harvie, Sean D Monahan, Debashish Roy, Allen G Li, Teri L Blevins, Amber E Paschal, Matt Waldheim, Eric C Bell, Anna Galperin, Jean-Rene Ella-Menye, Michael E Houston
We describe a novel, two-nanoparticle mRNA delivery system and show that it is highly effective as a means of intracellular enzyme replacement therapy (i-ERT) using a murine model of ornithine transcarbamylase deficiency (OTCD). Our Hybrid mRNA Technology delivery system (HMT) comprises an inert lipid nanoparticle that protects the mRNA from nucleases in the blood as it distributes to the liver and a polymer micelle that targets hepatocytes and triggers endosomal release of mRNA. This results in high-level synthesis of the desired protein specifically in the liver...
January 4, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29428387/characterization-of-a-new-oligoalginate-lyase-from-marine-bacterium-vibrio-sp
#12
Zuochen Yu, Benwei Zhu, Wenxia Wang, Haidong Tan, Heng Yin
A new oligoalginate lyase encoding gene, designed oal17A, was cloned from marine bacterium Vibrio sp. W13, and then expressed in Escherichia coli. The recombinant Oal17A was purified by NTA-Ni resin with maximal activity at 30 °C and pH 7.0. Oal17A exhibited broad substrate specificity, and preferred to degrade alginate than polyM or polyG into monosaccharide acid. The specific activity of Oal17A toward alginate, polyM and polyG was 21.14 U/mg, 12.31 U/mg and 7.43 U/mg, respectively. With features of high-level expression and broad substrate specificity, Oal17A would be a potential tool for alginate monomer production process of alginate utilizing for biofuels and bioethanol production...
February 8, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29427541/asymmetric-nano-textured-surfaces-influence-neuron-viability-and-polarity
#13
Andreea Belu, Mehmet Yilmaz, Elmar Neumann, Andreas Offenhäusser, Gokhan Demirel, Dirk Mayer
Three dimensional, nanostructured surfaces have attracted considerable attention in biomedical research since they have proven to represent a powerful platform to influence cell fate. In particular, nanorods and nanopillars possess great potential for the control of cell adhesion and differentiation, gene and biomolecule delivery, optical and electrical stimulation and recording, as well as cell patterning. Here, we investigate the influence of asymmetric poly(dichloro-p-xylene) (PPX) columnar films on the adhesion and maturation of cortical neurons...
February 10, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29427431/new-insights-into-the-function-and-global-distribution-of-polyethylene-terephthalate-pet-degrading-bacteria-and-enzymes-in-marine-and-terrestrial-metagenomes
#14
Dominik Danso, Christel Schmeisser, Jennifer Chow, Wolfgang Zimmermann, Ren Wei, Christian Leggewie, Xiangzhen Li, Terry Hazen, Wolfgang R Streit
Polyethylene terephthalate (PET) is one of the most important synthetic polymers used nowadays. Unfortunately, the polymers accumulate in nature and until now, no highly active enzymes are known that can degrade it at high velocity. Enzymes involved in PET degradation are mainly α/β-hydrolases like cutinases and related enzymes (E.C. 3.1.-). Currently, only a small number of such enzymes are well characterized. Within this work, a search algorithm was developed that identified 504 possible PET hydrolase candidate genes from various databases...
February 2, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29425184/bioreducible-polymer-micelles-based-on-acid-degradable-poly-ethylene-glycol-poly-amino-ketal-enhance-the-stromal-cell-derived-factor-1%C3%AE-gene-transfection-efficacy-and-therapeutic-angiogenesis-of-human-adipose-derived-stem-cells
#15
Tae-Jin Lee, Min Suk Shim, Taekyung Yu, Kyunghee Choi, Dong-Ik Kim, Soo-Hong Lee, Suk Ho Bhang
Adipose-derived stem cells (ADSCs) have the potential to treat ischemic diseases. In general, ADSCs facilitate angiogenesis by secreting various pro-angiogenic growth factors. However, transplanted ADSCs have a low therapeutic efficacy in ischemic tissues due to their poor engraftment and low viability. Stromal cell-derived factor-1α (SDF-1α) improves the survival rate of stem cells transplanted into ischemic regions. In this study, we developed acid-degradable poly(ethylene glycol)-poly(amino ketal) (PEG-PAK)-based micelles for efficient intracellular delivery of SDF-1α plasmid DNA...
February 9, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29423922/polymer-coated-viral-vectors-hybrid-nanosystems-for-gene-therapy
#16
REVIEW
Pratheppa Rajagopal, Sowmiya Duraiswamy, Swaminathan Sethuraman, Giridhara R Jayandharan, Uma Maheswari Krishnan
AIM: The advantages and critical aspects of nano-dimensional polymer-coated viral vector systems potentially applicable for gene delivery are reviewed in this article. DISCUSSION: Various viral and non-viral vectors have been explored for gene therapy. Viral gene transfer methods though highly efficient, are limited by their immunogenicity. Non-viral vectors have lower transfection efficiency due to their inability to escape from the endosome. To overcome these drawbacks, novel nanotechnology-mediated interventions that involve coating or modification of virus using polymers have emerged as a new paradigm in gene therapy...
February 8, 2018: Journal of Gene Medicine
https://www.readbyqxmd.com/read/29422010/berberine-encapsulated-plga-peg-nanoparticles-modulates-pcsk-9-in-hepg2-cells
#17
Chinedu Ochin, Mahdi Garelnabi
BACKGROUND: The developments of new parenteral approaches to target PCSK-9 for the treatment LDL-Cholesterol has yielded impressive results; and have shown significant decreases in the risk of mortality associated with hypercholesterolemia. However improved and convenient alternate approaches that exploit the beneficial drug target properties of PCSK-9 also need to be explored and developed. One such approach is the oral administration of Berberine using nanotechnology. METHODS: Nanoprecipitation encapsulation and physiochemical characterization of Berberine Chloride in PLGA-PEG-PLGA block copolymer has been developed and characterized in Hep-G2 cells using Berberine Chloride encapsulated nanoparticle (BC-NP)...
February 1, 2018: Cardiovascular & Hematological Disorders Drug Targets
https://www.readbyqxmd.com/read/29421514/gallocatechin-biosynthesis-via-a-flavonoid-3-5-hydroxylase-is-a-defense-response-in-norway-spruce-against-infection-by-the-bark-beetle-associated-sap-staining-fungus-endoconidiophora-polonica
#18
Almuth Hammerbacher, Bettina Raguschke, Louwrance P Wright, Jonathan Gershenzon
One of the best-studied defense responses to fungal infection in Norway spruce (Picea abies) is the biosynthesis of flavan-3-ols, which accumulate as monomers or polymers known as proanthocyanidins. The individual flavan-3-ol units consist of compounds with a 3',4'-dihydroxylated B ring [2,3-(trans)-(+)-catechin or 2,3-(cis)-(-)-epicatechin] and compounds with a 3',4',5'-trihydroxylated B ring [2,3 (trans)-(+)-gallocatechin or 2,3-(cis)-(-)-epigallocatechin]. While much is known about the biosynthesis and biological activity of catechin in Norway spruce, there is little comparable information about gallocatechin or epigallocatechin...
February 1, 2018: Phytochemistry
https://www.readbyqxmd.com/read/29416559/a-systematic-optimization-of-styrene-biosynthesis-in-escherichia-coli-bl21-de3
#19
Changqing Liu, Xiao Men, Hailin Chen, Meijie Li, Zhaorui Ding, Guoqiang Chen, Fan Wang, Haobao Liu, Qian Wang, Youshuang Zhu, Haibo Zhang, Mo Xian
Background: Styrene is a versatile commodity petrochemical used as a monomer building-block for the synthesis of many useful polymers. Although achievements have been made on styrene biosynthesis in microorganisms, several bottleneck problems limit factors for further improvement in styrene production. Results: A two-step styrene biosynthesis pathway was developed and introduced into Escherichia coli BL21(DE3). Systematic optimization of styrene biosynthesis, such as enzyme screening, codon and plasmid optimization, metabolic flow balance, and in situ fermentation was performed...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29415514/covalent-strategies-for-targeting-messenger-and-non-coding-rnas-an-updated-review-on-sirna-mirna-and-antimir-conjugates
#20
REVIEW
Santiago Grijalvo, Adele Alagia, Andreia F Jorge, Ramon Eritja
Oligonucleotide-based therapy has become an alternative to classical approaches in the search of novel therapeutics involving gene-related diseases. Several mechanisms have been described in which demonstrate the pivotal role of oligonucleotide for modulating gene expression. Antisense oligonucleotides (ASOs) and more recently siRNAs and miRNAs have made important contributions either in reducing aberrant protein levels by sequence-specific targeting messenger RNAs (mRNAs) or restoring the anomalous levels of non-coding RNAs (ncRNAs) that are involved in a good number of diseases including cancer...
February 6, 2018: Genes
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