keyword
MENU ▼
Read by QxMD icon Read
search

polymer and gene

keyword
https://www.readbyqxmd.com/read/29042138/managing-the-excessive-proliferation-of-glycogen-accumulating-organisms-in-industrial-activated-sludge-by-nitrogen-supplementation-a-fish-nanosims-approach
#1
Cristobal A Onetto, Kathryn L Eales, Paul Guagliardo, Matt R Kilburn, Joanna M Gambetta, Paul R Grbin
Defluviicoccus vanus-related glycogen accumulating organisms (GAO) regularly proliferate in industrial wastewater treatment plants handling high carbon but nitrogen deficient wastes. When GAO dominate, they are associated with poor performance, characterised by slow settling biomass and turbid effluents. Although their ecophysiology has been studied thoroughly in domestic waste treatment plants, little attention has been paid to them in aerobic industrial systems. In this study, the effect of nitrogen addition on GAO carbon metabolism was investigated during an 8h cycle...
October 2, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/29040803/synthesis-and-characterization-of-fatty-acid-grafted-chitosan-polymer-and-their-nanomicelles-for-non-viral-gene-delivery-applications
#2
Divya Sharma, Jagdish Singh
The aim of this study was to synthesize and characterize fatty acid-grafted-chitosan (fatty acid-g-CS) polymer and their nanomicelles for using as carriers for gene delivery. CS was hydrophobically modified using saturated fatty acids of increasing fatty acyl chain length. Carbodiimide along with N-hydroxysuccinimide was used for coupling carboxyl group of fatty acids with amine groups of CS. Proton nuclear magnetic resonance and Fourier transform-infrared spectroscopy were used to quantify fatty acyl substitution onto CS backbone...
October 17, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29038065/cationic-nanoemulsions-for-nucleic-acids-delivery
#3
REVIEW
Helder Ferreira Teixeira, Fernanda Bruxel, Michelle Fraga, Roselena Silvestri Schuh, Giovanni Konat Zorzi, Ursula Matte, Elias Fattal
Since the first clinical studies, knowledge in the field of gene therapy has advanced significantly, and these advances led to the development and subsequent approval of the first gene medicines. Although viral vectors-based products offer efficient gene expression, problems related to their safety and immune response have limited their clinical use. Thus, design and optimization of nonviral vectors is presented as a promising strategy in this scenario. Nonviral systems are nanotechnology-based products composed of polymers or lipids, which are usually biodegradable and biocompatible...
October 13, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29036344/comparison-of-the-in-vivo-induction-and-transmission-of-%C3%AE-synuclein-pathology-by-mutant-%C3%AE-synuclein-fibril-seeds-in-transgenic-mice
#4
Nicola J Rutherford, Jess-Karan S Dhillon, Cara J Riffe, Jasie K Howard, Mieu Brooks, Benoit I Giasson
Parkinson's disease is one of many neurodegenerative diseases termed synucleinopathies, neuropathologically defined by inclusions containing aggregated α-synuclein. α-synuclein gene (SNCA) mutations can directly cause autosomal dominant Parkinson's disease. In vitro studies demonstrated that SNCA missense mutations may either enhance or diminish α-synuclein aggregation but cross seeding of mutant and wild-type α-synuclein proteins appear to reduce aggregation efficiency. Here we extended these studies by assessing the effects of seeded α-synuclein aggregation in α-synuclein transgenic mice through intracerebral or peripheral injection of various mutant α-synuclein fibrils...
September 27, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/29035132/hybrid-lipid-polymer-nanoparticles-for-pulmonary-delivery-of-sirna-development-and-fate-upon-in-vitro-deposition-on-the-human-epithelial-airway-barrier
#5
Ivana d'Angelo, Gabriella Costabile, Estelle Durantie, Paola Brocca, Valeria Rondelli, Annapina Russo, Giulia Russo, Agnese Miro, Fabiana Quaglia, Alke Petri-Fink, Barbara Rothen-Rutishauser, Francesca Ungaro
BACKGROUND: Nowadays, the downregulation of genes involved in the pathogenesis of severe lung diseases through local siRNA delivery appears an interesting therapeutic approach. In this study, we propose novel hybrid lipid-polymer nanoparticles (hNPs) consisting of poly(lactic-co-glycolic) acid (PLGA) and dipalmitoyl phosphatidylcholine (DPPC) as siRNA inhalation system. METHODS: A panel of DPPC/PLGA hNPs was prepared by emulsion/solvent diffusion and fully characterized...
October 16, 2017: Journal of Aerosol Medicine and Pulmonary Drug Delivery
https://www.readbyqxmd.com/read/29034997/everything-you-always-wanted-to-know-about-poly-l-lysine-dendrigrafts-but-were-afraid-to-ask
#6
Jean-Patrick Francoia, Laurent Vial
Less than a decade ago, dendrigrafts of poly-L-lysine (DGLs) joined the family of polycationic dendritic macromolecules. Resulting from the iterative polycondensation of a N-carboxyanhydride in water, four generations of the dendrigraft can be obtained on a multi-gram scale and without chromatographic purification. DGLs share features with both dendrimers and hyperbranched polymers, but turned out to have unique biophysical and bioactive properties. The macromolecules - in their native form or functionalized - have been extensively characterized by various analytical and computational methods, and already found numerous applications in the biomedical field such as drug and gene delivery, biomaterials, tissue engineering, bioimaging, and biosensing...
October 16, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29032473/oligosaccharide-biotechnology-an-approach-of-prebiotic-revolution-on-the-industry
#7
REVIEW
Mario Cezar Rodrigues Mano, Iramaia Angélica Neri-Numa, Juliana Bueno da Silva, Bruno Nicolau Paulino, Marina Gabriel Pessoa, Gláucia Maria Pastore
Oligosaccharides are polymers with two to ten monosaccharide residues which have sweetener functions and sensory characteristics, in addition to exerting physiological effects on human health. The ones called nondigestible exhibit a prebiotic behavior being fermented by colonic microflora or stimulating the growth of beneficial bacteria, playing roles in the immune system, protecting against cancer, and preventing cardiovascular and metabolic issues. The global prebiotics market is expected to grow around 12...
October 14, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29026706/development-of-novel-drug-eluting-stents-for-acute-myocardial-infarction
#8
REVIEW
Doo Sun Sim, Myung Ho Jeong
Delayed arterial healing at culprit sites after drug-eluting stent (DES) placement for acute myocardial infarction (AMI) is associated with increased risk of late stent thrombosis. The Korea Acute Myocardial Infarction Registry was established in commemoration of the 50(th) anniversary of Korea Circulation Society. Between November 2005 and December 2016, more than 62,000 patients were registered from 50 primary percutaneous coronary intervention (PCI) centers in Korea. DES in AMI may be safe and effective, however, there is concern about increased stent thrombosis after DES implantation in AMI patients, requiring longer-term dual anti-platelet therapy to reduce the risk of late stent thrombosis...
September 2017: Chonnam Medical Journal
https://www.readbyqxmd.com/read/29024311/collagen-inducing-biologization-of-prosthetic-material-for-hernia-repair-polypropylene-meshes-coated-with-polyp-collagen
#9
Maximilian Ackermann, Xiaohong Wang, Shunfeng Wang, Meik Neufurth, Heinz C Schröder, Friedrich-Eckart Isemer, Werner E G Müller
Prostethic mesh material such as nonabsorbable polypropylene used in open and laparoscopic hernia repair are characterized by controllable mechanical properties but may elicit undesirable physiological reactions due to the nonphysiological inert polymer material. We succeeded in developing a biocompatible coating for these meshes, based on a physiological inorganic polymer, polyphosphate (polyP) that is morphogenetically active and used as a metabolic energy source, and a collagen matrix. The polyP/collagen hydrogel material was prepared by a freeze-extraction method, with amorphous Ca-polyP microparticles...
October 10, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29021549/protease-resistance-of-porcine-acidic-mammalian-chitinase-under-gastrointestinal-conditions-implies-that-chitin-containing-organisms-can-be-sustainable-dietary-resources
#10
Eri Tabata, Akinori Kashimura, Satoshi Wakita, Misa Ohno, Masayoshi Sakaguchi, Yasusato Sugahara, Yasutada Imamura, Shiro Seki, Hitoshi Ueda, Vaclav Matoska, Peter O Bauer, Fumitaka Oyama
Chitin, a polymer of N-acetyl-D-glucosamine (GlcNAc), is a major structural component in chitin-containing organism including crustaceans, insects and fungi. Mammals express two chitinases, chitotriosidase (Chit1) and acidic mammalian chitinase (AMCase). Here, we report that pig AMCase is stable in the presence of other digestive proteases and functions as chitinolytic enzyme under the gastrointestinal conditions. Quantification of chitinases expression in pig tissues using quantitative real-time PCR showed that Chit1 mRNA was highly expressed in eyes, whereas the AMCase mRNA was predominantly expressed in stomach at even higher levels than the housekeeping genes...
October 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29020919/cell-wall-metabolism-and-hexose-allocation-contribute-to-biomass-accumulation-in-high-yielding-extreme-segregants-of-a-saccharum-interspecific-f2-population
#11
Ching Man Wai, Jisen Zhang, Tyler C Jones, Chifumi Nagai, Ray Ming
BACKGROUND: Sugarcane is an emerging dual-purpose biofuel crop for energy and sugar production, owing to its rapid growth rate, high sucrose storage in the stems, and high lignocellulosic yield. It has the highest biomass production reaching 1.9 billion tonnes in 2014 worldwide. RESULTS: To improve sugarcane biomass accumulation, we developed an interspecific cross between Saccharum officinarum 'LA Purple' and Saccharum robustum 'MOL5829'. Selected F1 individuals were self-pollinated to generate a transgressive F2 population with a wide range of biomass yield...
October 11, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29020443/the-solution-conformation-of-polymer-brushes-determines-their-interactions-with-dna-and-transfection-efficiency
#12
Mahentha Krishnamoorthy, Danyang Li, Amir S Sharili, Tina Gulin-Sarfraz, Jessica M Rosenholm, Julien Ezra Gautrot
Polymer brush-functionalised nanomaterials offer interesting features for the design of gene delivery vectors as their physico-chemical and structural properties can be designed independently of the chemistry, size and shape of the nanomaterial core. However, little is known of the parameters regulating the adsorption and infiltration of DNA molecules at the surface of positively charged polymer brushes, despite the importance of such processes for gene delivery. Here we investigate the role of the molecular environment (e...
October 11, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/29018466/differential-lipid-composition-and-gene-expression-in-the-semi-russeted-cox-orange-pippin-apple-variety
#13
Sylvain Legay, Emmanuelle Cocco, Christelle M André, Cédric Guignard, Jean-Francois Hausman, Gea Guerriero
Russeting is characterized by a particular rough and brown phenotype, which is mainly due to the accumulation of suberin in the inner part of the epidermal cell walls. In our previous bulk transcriptomic analysis, comparing fully russeted, and waxy apple varieties, showed, in apple fruit skin, a massive decreased expression of cutin, wax and some pentacyclic triterpene biosynthesis genes in the russeted varieties, with an expected concomitant enhanced expression of the suberin biosynthetic genes. In the present work, we performed a deep investigation of the aliphatic composition of the cutin, suberin, waxes, and triterpenes in the waxy and russeted patches of the semi-russeted apple variety "Cox Orange Pippin...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29018117/reciprocal-prioritization-to-dietary-glycans-by-gut-bacteria-in-a-competitive-environment-promotes-stable-coexistence
#14
Yunus E Tuncil, Yao Xiao, Nathan T Porter, Bradley L Reuhs, Eric C Martens, Bruce R Hamaker
When presented with nutrient mixtures, several human gut Bacteroides species exhibit hierarchical utilization of glycans through a phenomenon that resembles catabolite repression. However, it is unclear how closely these observed physiological changes, often measured by altered transcription of glycan utilization genes, mirror actual glycan depletion. To understand the glycan prioritization strategies of two closely related human gut symbionts, Bacteroides ovatus and Bacteroides thetaiotaomicron, we performed a series of time course assays in which both species were individually grown in a medium with six different glycans that both species can degrade...
October 10, 2017: MBio
https://www.readbyqxmd.com/read/29017823/erythroblast-differentiation-at-spleen-in-q137e-mutant-ribosomal-protein-s19-gene-knock-in-c57bl-6j-mice
#15
Koji Yamanegi, Naoko Yamada, Keiji Nakasho, Hiroshi Nishiura
We recently found that erythroblast-like cells derived from human leukaemia K562 cells express C5a receptor (C5aR) and produce its antagonistic and agonistic ligand ribosomal protein S19 (RP S19) polymer, which is cross-linked between K122 and Q137 by tissue transglutaminases. RP S19 polymer binds to the reciprocal C5aRs on erythroblast-like cells and macrophage-like cells derived from human monocytic THP-1 cells and promotes differentiation into reticulocyte-like cells through enucleation in vitro. To examine the roles of RP S19 polymer in mouse erythropoiesis, we prepared Q137E mutant RP S19 gene knock-in C57BL/6J mice...
October 5, 2017: Immunobiology
https://www.readbyqxmd.com/read/29017784/arginine-rich-cross-linking-peptides-with-different-sv40-nuclear-localization-signal-content-as-vectors-for-intranuclear-dna-delivery
#16
Mariia Bogacheva, Anna Egorova, Anna Slita, Marianna Maretina, Vladislav Baranov, Anton Kiselev
The major barriers for intracellular DNA transportation by cationic polymers are their toxicity, poor endosomal escape and inefficient nuclear uptake. Therefore, we designed novel modular peptide-based carriers modified with SV40 nuclear localization signal (NLS). Core peptide consists of arginine, histidine and cysteine residues for DNA condensation, endosomal escape promotion and interpeptide cross-linking, respectively. We investigated three polyplexes with different NLS content (10 mol%, 50 mol% and 90 mol% of SV40 NLS) as vectors for intranuclear DNA delivery...
October 3, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29017070/conducting-electrospun-fibres-with-polyanionic-grafts-as-highly-selective-label-free-electrochemical-biosensor-with-a-low-detection-limit-for-non-hodgkin-lymphoma-gene
#17
Thomas E Kerr-Phillips, Nihan Aydemir, Eddie Wai Chi Chan, David Barker, Jenny Malmstro M, Cedric Plesse, Jadranka Travas-Sejdic
A highly selective, label-free sensor for the non-Hodgkin lymphoma gene, with an aM detection limit, utilizing electrochemical impedance spectroscopy (EIS) is presented. The sensor consists of a conducting electrospun fibre mat, surface-grafted with poly(acrylic acid) (PAA) brushes and a conducting polymer sensing element with covalently attached oligonucleotide probes. The sensor was fabricated from electrospun NBR rubber, embedded with poly(3,4-ethylenedioxythiophene) (PEDOT), followed by grafting poly(acrylic acid) brushes and then electrochemically polymerizing a conducting polymer monomer with ssDNA probe sequence pre-attached...
September 28, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28987032/functionalized-pva-silk-blended-nanofibrous-mats-promote-diabetic-wound-healing-via-regulation-of-extracellular-matrix-and-tissue-remodeling
#18
Dimple Chouhan, G Janani, Bijayashree Chakraborty, Samit K Nandi, Biman B Mandal
Chronic cutaneous ulcers, a complex pathophysiological diabetic condition, represent a critical clinical challenge in the current diabetes mellitus pandemic. Consequently, there is a compelling need for bioactive dressings which can trigger healing processes for complete wound repair. Silk fibroin (SF), a natural protein polymer from mulberry and non-mulberry silkworms, has properties that support accelerated wound healing rate. SF from non-mulberry variety possesses additional cell-binding motifs (RGD), offering cell-material interactions...
October 7, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28982015/synthesis-of-polyhydroxyalkanoates-phas-from-vegetable-oils-and-free-fatty-acids-by-wild-type-and-mutant-strains-of-pseudomonas-chlororaphis
#19
Parveen Kumar Sharma, Rifat Munir, Teresa de Kievit, David B Levin
Pseudomonas chlororaphis strain PA23 was isolated from soybean roots as a plant growth-promoting rhizobacterium (PGPR) and secretes a wide-range of compounds, including the antibiotics phenazine-1-caroxymide (PCA), pyrrolnitrin, and 2-hydroxyphenazine. We have determined that P. chlororaphis PA23 can synthesize medium chain length polyhydroxyalkanoate (PHA) polymers utilizing free fatty acids, such as octanoic acid and nonanoic acid, as well as vegetable oils as sole carbon sources. Genome analysis identified a pha operon containing 7 genes in P...
October 5, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28976121/strategies-used-in-the-clinical-trials-of-gene-therapy-for-cancer
#20
Thekkuttuparambil Ananthanarayanan Ajith
Advances in understanding and manipulating genes have set the stage for scientists to alter a person's genetic material to prevent or treat diseases. Over the past decade, somatic gene therapy has been increasingly applied in clinical trials where the genetic material (DNA and RNA) introduced into a person's cell. Mutation and inactivation of the tumor suppressor genes are the unified concept of the development of tumor in humans. Therefore, researchers have discovered potential of gene therapies in the treatment of cancer...
September 2017: Journal of Experimental Therapeutics & Oncology
keyword
keyword
5786
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"