keyword
MENU ▼
Read by QxMD icon Read
search

polymer and gene

keyword
https://www.readbyqxmd.com/read/28431248/spontaneous-chitin-accumulation-in-airways-and-age-related-fibrotic-lung-disease
#1
Steven J Van Dyken, Hong-Erh Liang, Ram P Naikawadi, Prescott G Woodruff, Paul J Wolters, David J Erle, Richard M Locksley
The environmentally widespread polysaccharide chitin is degraded and recycled by ubiquitous bacterial and fungal chitinases. Although vertebrates express active chitinases from evolutionarily conserved loci, their role in mammalian physiology is unclear. We show that distinct lung epithelial cells secrete acidic mammalian chitinase (AMCase), which is required for airway chitinase activity. AMCase-deficient mice exhibit premature morbidity and mortality, concomitant with accumulation of environmentally derived chitin polymers in the airways and expression of pro-fibrotic cytokines...
April 20, 2017: Cell
https://www.readbyqxmd.com/read/28428056/recent-development-of-synthetic-nonviral-systems-for-sustained-gene-delivery
#2
REVIEW
Yonggang Xiang, Nwe Nwe Linn Oo, Jin Ping Lee, Zibiao Li, Xian Jun Loh
Sustained gene delivery is of particular importance today because it circumvents the need for repeated therapeutic administration and provides spatial and temporal control of the release profile. Better understanding of the genetic basis of diseases and advances in gene therapy have propelled significant research on biocompatible gene carriers for therapeutic purposes. Varied biodegradable polymer-based architectures have been used to create new compositions with unique properties suitable for sustained gene delivery...
April 17, 2017: Drug Discovery Today
https://www.readbyqxmd.com/read/28423276/supramolecular-%C3%AE-sheets-stabilized-protein-nanocarriers-for-drug-delivery-and-gene-transfection
#3
Yuan Ping, Dawei Ding, Ricardo Almeida Neves Sampayo Ramos, Harini Mohanram, Kanagavel Deepankumar, Jianqing Gao, Guping Tang, Ali Miserez
Suckerin proteins, recently discovered in the sucker ring teeth of squids, represent a family of promising structural biomacromolecules that can form supramolecular networks stabilized by nanoconfined β-sheets. Exploiting this feature as well as their specific amino acid composition, we demonstrate that artificial suckerin-19 (S-19) can be engineered into nanocarriers for efficient drug delivery and gene transfection in vitro and in vivo. First, we demonstrated that S-19 is self-assembled into β-sheet stabilized nanoparticles with controlled particle sizes of 100-200 nm that are able to encapsulate hydrophobic drugs for pH-dependent release in vitro, and that could effectively inhibit tumor growth in vivo...
April 19, 2017: ACS Nano
https://www.readbyqxmd.com/read/28423213/systemic-tumor-targeted-sodium-iodide-symporter-nis-gene-therapy-of-hepatocellular-carcinoma-mediated-by-b6-peptide-polyplexes
#4
Sarah Urnauer, Kathrin Klutz, Geoffrey K Grünwald, Stephan Morys, Nathalie Schwenk, Christian Zach, Franz-Josef Gildehaus, Wolfgang Roedl, Manfred Ogris, Ernst Wagner, Christine Spitzweg
BACKGROUND: Non-viral polymer-based gene transfer represents an adaptable system for tumor-targeted gene therapy, as various design strategies of shuttle systems together with the mechanistic concept of active tumor targeting lead to improved gene delivery vectors resulting in higher tumor specificity, efficacy and safety. METHODS: Using the sodium iodide symporter (NIS) as theranostic gene, non-viral gene delivery vehicles based on linear polyethylenimine (LPEI), polyethylene glycol (PEG) and coupled with the synthetic peptide B6 (LPEI-PEG-B6), which specifically binds to tumor cells, were investigated in a hepatocellular carcinoma (HCC) xenograft model for tumor-selectivity and transduction efficiency...
April 19, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28423187/characterization-of-the-binding-capacity-of-mercurial-species-in-lactobacillus-strains
#5
Cristina Alcántara, Carlos Jadán-Piedra, Dinoraz Vélez, Vicenta Devesa, Manuel Zúñiga, Vicente Monedero
BACKGROUND: Metal sequestration by bacteria has been proposed as a strategy to counteract metal contamination in foodstuffs. Lactobacilli can interact with metals, but studies with important foodborne metals such as inorganic [Hg(II)] or organic (CH3 Hg) mercury are lacking. Lactobacilli were evaluated for their potential to bind these contaminants and the nature of the interaction was assessed by the use of metal competitors, chemical and enzymatical treatments and mutants affected in the cell-wall structure...
April 19, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28421280/systems-biology-of-seeds-deciphering-the-molecular-mechanisms-of-seed-storage-dormancy-and-onset-of-germination
#6
EDITORIAL
Nese Sreenivasulu
Seeds are heterogeneous storage reserves with wide array of storage compounds that include various soluble carbohydrates, starch polymer, storage proteins and lipids. These stored reserves comprise 70% of the world's caloric intake in the form of food and animal feed produced through sustainable agriculture, which contributes to food and nutritional security. Seed systems biology remains an enigmatic subject in understanding seed storage processes, maturation and pre-germinative metabolism. The reviews and research articles covered in this special issue of Plant Cell Reports highlight recent advances made in the area of seed biology that cover various systems biology applications such as gene regulatory networks, metabolomics, epigenetics and the role of micro-RNA in seed development...
April 18, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28416815/in-situ-programming-of-leukaemia-specific-t-cells-using-synthetic-dna-nanocarriers
#7
Tyrel T Smith, Sirkka B Stephan, Howell F Moffett, Laura E McKnight, Weihang Ji, Diana Reiman, Emmy Bonagofski, Martin E Wohlfahrt, Smitha P S Pillai, Matthias T Stephan
An emerging approach for treating cancer involves programming patient-derived T cells with genes encoding disease-specific chimeric antigen receptors (CARs), so that they can combat tumour cells once they are reinfused. Although trials of this therapy have produced impressive results, the in vitro methods they require to generate large numbers of tumour-specific T cells are too elaborate for widespread application to treat cancer patients. Here, we describe a method to quickly program circulating T cells with tumour-recognizing capabilities, thus avoiding these complications...
April 17, 2017: Nature Nanotechnology
https://www.readbyqxmd.com/read/28416401/polymeric-nanoparticles-for-sirna-delivery-production-and-applications
#8
Gennara Cavallaro, Carla Sardo, Emanuela Fabiola Craparo, Barbara Porsio E Gaetano Giammona
Gene therapy through the use of siRNA and a polymeric carrier are becoming an efficient therapeutic option to conventional pharmaceutical formulations for the treatment of deadly diseases, such as cancer, pulmonary, ocular and neurodegenerative diseases. However, several considerations regarding the stability, formulation, and efficacy have to be faced up until these systems could be considered to be a marketable pharmaceutical products for to extend siRNA application to clinical practice. This review is focused on the key challenges of siRNA therapeutics, with special attention on the faced obstacles and on the formulation-related difficulties, providing a list of requirements needed for obtaining an ideal carrier for siRNA...
April 14, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28415444/formulation-of-thermoreversible-gel-of-cranberry-juice-concentrate-evaluation-biocompatibility-studies-and-its-antimicrobial-activity-against-periodontal-pathogens
#9
Rajeshwari H R, Dinesh Dhamecha, Satveer Jagwani, Dhanashree Patil, Satisha Hegde, Raksha Potdar, Renuka Metgud, Sunil Jalalpure, Subarna Roy, Kiran Jadhav, Neeraj Kumar Tiwari, Sravani Koduru, Shweta Hugar, Suneel Dodamani
The present work aims to investigate the efficacy of thermoreversible gel of cranberry juice concentrate (CJC) as local drug delivery for the treatment of periodontitis. CJC was initially tested for its antimicrobial activities like MIC, MBC, antiadhesion, antibiofilm and time kill assay against the panel of organisms (S. mutans (SM), E. faecalis (EF), A. actinomycetemcomitans (AA), P. gingivalis (PG), T. forsythia (TF)) responsible for periapical and periodontal infections. Antimicrobial activity of CJC showed MIC value of 50mg/ml and MBC value of 100mg/ml with desirable antiadhesion (83-90%) and antibiofilm activity (70-85%)...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28411641/nanoarmoring-of-proteins-by-conjugation-to-block-copolymer-micelles
#10
Nisaraporn Suthiwangcharoen, Ramanathan Nagarajan
The creation of polymer nanoparticles with protein functionality is of great interest to many applications such as targeted drug or gene delivery, diagnostic imaging, cancer theranostics, delivery of protein therapeutics, sensing chemical and biomolecular analytes in complex environments, and design of protective clothing resembling a second skin. Many approaches to achieving this goal are being explored in the current literature. In this chapter, we describe a relatively simple and flexible approach of conjugating the protein to an amphiphilic block copolymer and creating polymer nanoparticles with protein functionality by taking advantage of the intrinsic self-assembly behavior of the amphiphilic block copolymer...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28407530/redox-responsive-nanocarriers-for-drug-and-gene-co-delivery-based-on-chitosan-derivatives-modified-mesoporous-silica-nanoparticles
#11
Jian-Tao Lin, Zong-Kun Liu, Qing-Ling Zhu, Xiao-Hui Rong, Cui-Ling Liang, Jie Wang, Dong Ma, Jing Sun, Guan-Hai Wang
Stimuli-responsive nanocarriers for anticancer drug and gene co-delivery are promising strategy in cancer therapy. The ultimate goal is to deliver high local concentration of therapeutic agents with no premature release and result in synergistic effects for combination therapies. In this work, we developed a redox stimuli-responsive and synergistic co-delivery system for anticancer drug DOX and p53 gene for potential cancer therapy. A dendronized chitosan derivative (CP) as a "gatekeeper" to control release the drug was used to modify MSNs via a disulfide linker and improve the gene transfection efficiency...
April 5, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28404942/a-novel-bioactive-osteogenesis-scaffold-delivers-ascorbic-acid-%C3%AE-glycerophosphate-and-dexamethasone-in-vivo-to-promote-bone-regeneration
#12
Chao Wang, Xuecheng Cao, Yongxian Zhang
Ascorbic acid, β-glycerophosphate, and dexamethasone have been used in osteogenesis differentiation medium for in vitro cell culture, nothing is known for delivering these three bioactive compounds in vivo. In this study, we synthesized a novel bioactive scaffold by combining these three compounds with a lysine diisocyanate-based polyurethane. These bioactive compounds were released from the scaffold during the degradation process. The cell culture showed that the sponge-like structure in the scaffold was critical in providing a large surface area to support cell growth and all degradation products of the polymer were non-toxic...
February 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28400331/four-paralog-gelsolin-genes-are-differentially-expressed-in-the-earthworm-lumbricus-terrestris
#13
Prasath Thiruketheeswaran, Paul Thomalla, Evelyn Krüger, Horst Hinssen, Jochen D'Haese
We have identified and characterized four distinct variants of the gelsolin-related protein (EWAM P1-P4) in the earthworm L. terrestris. All of these proteins biochemically qualify as gelsolins since they sever actin filaments in a calcium dependent manner. P1, P2 and P3 are present in the Lumbricus body wall muscle whereas in the gizzard muscle P3 and P4 were found. P1-P4 are encoded by four paralog genes and are differentially expressed in various muscle cell tissues. While the genes for P1 and P2 contain one intron, there was no intron in both P3 and P4 genes...
April 8, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28399108/decreased-levels-of-baseline-and-drug-induced-tubulin-polymerisation-are-hallmarks-of-resistance-to-taxanes-in-ovarian-cancer-cells-and-are-associated-with-epithelial-to-mesenchymal-transition
#14
George E Duran, Yan C Wang, François Moisan, E Brian Francisco, Branimir I Sikic
BACKGROUND: ABCB1 expression is uncommon in ovarian cancers in the clinical setting so we investigated non-MDR mechanisms of resistance to taxanes. METHODS: We established eight taxane-resistant variants from the human ovarian carcinoma cell lines A2780/1A9, ES-2, MES-OV and OVCAR-3 by selection with paclitaxel or docetaxel, with counter-selection by the transport inhibitor valspodar. RESULTS: Non-MDR taxane resistance was associated with reduced intracellular taxane content compared to parental controls, and cross-resistance to other microtubule stabilising drugs...
April 11, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28397966/synthesis-and-assembly-of-click-nucleic-acid-containing-peg-plga-nanoparticles-for-dna-delivery
#15
Albert Harguindey, Dylan W Domaille, Benjamin D Fairbanks, Justine Wagner, Christopher N Bowman, Jennifer N Cha
Co-delivery of both chemotherapy drugs and siRNA from a single delivery vehicle can have a significant impact on cancer therapy due to the potential for overcoming issues such as drug resistance. However, the inherent chemical differences between charged nucleic acids and hydrophobic drugs have hindered entrapment of both components within a single carrier. While poly(ethylene glycol)-block-poly(lactic-co-glycolic acid) (PEG-PLGA) copolymers have been used successfully for targeted delivery of chemotherapy drugs, loading of DNA or RNA has been poor...
April 11, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28397188/brain-targeted-polymers-for-gene-delivery-in-the-treatment-of-brain-diseases
#16
REVIEW
Yifei Lu, Chen Jiang
Gene therapies have become a promising strategy for treating neurological disorders, such as brain cancer and neurodegenerative diseases, with the help of molecular biology interpreting the underlying pathological mechanisms. Successful cellular manipulation against these diseases requires efficient delivery of nucleic acids into brain and further into specific neurons or cancer cells. Compared with viral vectors, non-viral polymeric carriers provide a safer and more flexible way of gene delivery, although suffering from significantly lower transfection efficiency...
April 2017: Topics in Current Chemistry (Journal)
https://www.readbyqxmd.com/read/28393560/evaluating-polyethyleneimine-dna-nanoparticles-mediated-damage-to-cellular-organelles-using-endoplasmic-reticulum-stress-profile
#17
Maryam Dabbaghi, Reza Kazemi Oskuee, Khadijeh Hashemi, Amir Afkhami Goli
Gene therapy has emerged as an influential tool for treating the genetic and specific acquired disorders. Among all kinds of gene delivery systems, the cationic polymer polyethyleneimine (PEI) is considered as a promising non-viral gene delivery vector, although there are still concerns about its magnitude of cytotoxicity. While any cell insult leads to unfolded/misfolded protein accumulation and its consequent unfold protein response, evaluating the expression profile of ER-stress genes would be a sensitive indicator of cell stress...
April 10, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28391040/pegylated-composite-nanoparticles-of-plga-and-polyethylenimine-for-safe-and-efficient-delivery-of-pdna-to-lungs
#18
Atul Kolte, Sushilkumar Patil, Pierre Lesimple, John W Hanrahan, Ambikanandan Misra
Achieving stable, efficient and non-toxic pulmonary gene delivery is most challenging requirement for successful gene therapy to lung. Composite nanoparticles (NPs) of the poly(lactic-co-glycolic acid) (PLGA) and cationic polymer polyethyleneimine (PEI) is an efficient alternative to viral and liposomal vectors for the pulmonary delivery of pDNA. NPs with different weight ratios (0-12.5%w/w) of PLGA/PEI were prepared and characterized for size, morphology, surface charge, pDNA loading and in vitro release. The in vitro cell uptake and transfection studies in the CFBE41o-cell line revealed that NPs with 10% w/w PEI were more efficient but they exhibited significant cytotoxicity in MTT assays, challenging the safety of this formulation...
April 6, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28388842/polyanionic-antimicrobial-membranes-an-experimental-and-theoretical-study
#19
Jiangna Guo, Qiming Xu, Rongwei Shi, Zhiqiang Zheng, Hailei Mao, Feng Yan
Polycationic polymers have been widely used as antimirobial materials because of their broad spectrum activity and potential use as new antibiotics. Herein, we report the synthesis of polyanionic antimicrobial membranes by in situ photo-cross-linking of a sulfate based anionic monomer and followed by cation-exchange with organic (quaternary ammonium or imidazolium) or metal (Ag +, Cu (2+) , Fe (3+), Zn (2+), Na (+), K (+)) cations. The resultant polyanionic membranes show high and broad spectrum antibacterial activities against both bacteria (Escherichia coli (E...
April 7, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28376367/injectable-nanofibrous-spongy-microspheres-for-nr4a1-plasmid-dna-transfection-to-reverse-fibrotic-degeneration-and-support-disc-regeneration
#20
Ganjun Feng, Zhanpeng Zhang, Ming Dang, Xiaojin Zhang, Yasmine Doleyres, Yueming Song, Di Chen, Peter X Ma
Safe and efficient gene therapy is highly desired for controlling pathogenic fibrosis of nucleus pulposus (NP) tissue, which would result in intervertebral disc (IVD) degeneration and disability if left untreated. In this work, a hyperbranched polymer (HP) with high plasmid DNA (pDNA) binding affinity and negligible cytotoxicity is synthesized, which can self-assemble into nano-sized polyplexes with a "double shell" structure that can transfect pDNA into NP cells with very high efficiency. These polyplexes are then encapsulated in biodegradable nanospheres (NS) to enable two-stage delivery: 1) temporally-controlled release of pDNA-carrying polyplexes and 2) highly efficient delivery of pDNA into cells by the released polyplexes...
March 24, 2017: Biomaterials
keyword
keyword
5786
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"