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https://www.readbyqxmd.com/read/29165900/development-of-a-pre-column-derivatization-hplc-method-with-diode-array-detection-for-the-determination-of-amino-sugars-in-peat-and-soil-humic-acids
#1
Erik Beňo, Róbert Góra, Milan Hutta
The work is focused on the development of a high-performance liquid chromatography method with diode-array detection for the separation and quantitation of the three most abundant amino sugars; d-glucosamine, d-galactosamine, and d-mannosamine. The high-performance liquid chromatography separation was carried out by reversed-phase chromatography on Chromolith Performance RP-18e monolithic column after acid hydrolysis (5 M HCl) and pre-column derivatization of samples using diethyl ethoxymethylenemalonate. Gradient elution and a mobile phase composed of ammonium formate buffer solution (10 mmol L(-1) , pH 3...
November 22, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/29165829/simultaneous-determination-and-method-validation-of-clethodim-and-its-metabolites-clethodim-sulfoxide-and-clethodim-sulfone-in-tobacco-by-lc-ms-ms
#2
Fei Wang, Guoqiang Yang, Jin Xu, Weiwei Yu, Lihong Shi, Song Zeng, Lingzhu Chen, Deyu Hu, Kankan Zhang
A simple method was developed and validated for the simultaneous determination of clethodim, clethodim sulfoxide, and clethodim sulfone in soil and tobacco by liquid chromatography with tandem mass spectrometry. The three target compounds were extracted from tobacco and soil with acetonitrile, and the extracts were purified using octadecyl silane. The proposed method showed satisfactory linearity (R(2) ≥ 0.9973) for the target compounds. The limits of detection and quantitation of the three analytes in all matrices were 0...
November 22, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29165649/a-comprehensive-study-of-residential-geogenic-and-water-radon-in-the-north-area-of-mure%C3%A5-county-romania
#3
Botond Papp, Alexandra Cucos Dinu, Constantin Cosma
This study presents results of a complex survey about residential, soil and water radon in the North of Mureş county (Romania). Indoor radon measurements were performed by using CR-39 track detectors, while radon concentrations in soil and in water were measured by using the LUK3C device and accessories. The indoor radon concentrations of 157 houses ranged from 9 to 414 Bq m-3, with an arithmetic mean of 131 Bq m-3 and a geometric mean of 105 Bq m-3. In ~3.2% of the investigated houses exceed the recommended reference level of 300 Bq m-3...
November 18, 2017: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/29165439/proteomic-signatures-differentiating-bacillus-anthracis-sterne-sporulation-on-soil-relative-to-laboratory-media
#4
D S Wunschel, J R Hutchison, B L Deatherage Kaiser, E D Merkley, B M Hess, A Lin, M G Warner
The process of sporulation is vital for the stability and infectious cycle of Bacillus anthracis. The spore is the infectious form of the organism and therefore relevant to biodefense. While the morphological and molecular events occurring during sporulation have been well studied, the influence of growth medium and temperature on the proteins expressed in sporulated cultures is not well understood. Understanding the features of B. anthracis sporulation specific to natural vs. laboratory production will address an important question in microbial forensics...
November 22, 2017: Analyst
https://www.readbyqxmd.com/read/29165218/molecular-identification-of-trichuris-suis-and-trichuris-trichiura-eggs-in-human-populations-from-thailand-lao-pdr-and-myanmar
#5
Issarapong Phosuk, Oranuch Sanpool, Tongjit Thanchomnang, Lakkhana Sadaow, Rutchanee Rodpai, Witthaya Anamnart, Penchom Janwan, Adulsak Wijit, Sakhone Laymanivong, Win Pa Pa Aung, Pewpan M Intapan, Wanchai Maleewong
Trichuris trichiura is a soil-transmitted helminth infecting human populations globally. Human cases caused by Trichuris suis and Trichuris vulpis have also been reported. Molecular identifications of Trichuris species infecting human populations in Lao PDR and Myanmar are lacking. Here, we explored molecular data obtained from Trichuris eggs recovered from human fecal samples from these countries and compared these with new and existing data from Thailand. Nuclear ribosomal DNA (18S and ITS2) sequences were amplified from Trichuris eggs and sequenced...
November 20, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29165214/fecal-contamination-on-produce-from-wholesale-and-retail-food-markets-in-dhaka-bangladesh
#6
Angela R Harris, Mohammad Aminul Islam, Leanne Unicomb, Alexandria B Boehm, Stephen Luby, Jennifer Davis, Amy J Pickering
Fresh produce items can become contaminated with enteric pathogens along the supply chain at the preharvest (e.g., irrigation water, soil, fertilizer) or postharvest (e.g., vendor handling or consumer handling) stages. This study assesses the concentrations of fecal indicator bacteria Escherichia coli, enterococci (ENT), and Bacteriodales on surfaces of carrots, eggplants, red amaranth leaves, and tomatoes obtained from both a wholesale market (recently harvested) and neighborhood retail markets in Dhaka, Bangladesh...
November 20, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29164461/seasonal-toxicity-variation-in-light-textured-soil-amended-with-urban-sewage-sludge-interaction-effect-on-cadmium-nickel-and-phytotoxicity
#7
Rahma Inès Zoghlami, Helmi Hamdi, Khaoula Boudabbous, Sarra Hechmi, Mohamed Naceur Khelil, Naceur Jedidi
Sewage sludge is increasingly used as an organic amendment to agricultural soils, especially to soils containing little organic matter. However, little is known on the impact of this biowaste on seasonal changes of nickel and cadmium toxicity in a sandy loam soil. Accordingly, the aim of this field-scale study was to evaluate the seasonal phytotoxicity according to Cd, Ni, and dehydrogenase variation in an agricultural soil during two successive annual amendments with increasing amounts of urban sludge (0, 40, 80, and 120 t ha(-1) year(-1))...
November 21, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29163572/root-and-rhizosphere-bacterial-phosphatase-activity-varies-with-tree-species-and-soil-phosphorus-availability-in-puerto-rico-tropical-forest
#8
Kristine G Cabugao, Collin M Timm, Alyssa A Carrell, Joanne Childs, Tse-Yuan S Lu, Dale A Pelletier, David J Weston, Richard J Norby
Tropical forests generally occur on highly weathered soils that, in combination with the immobility of phosphorus (P), often result in soils lacking orthophosphate, the form of P most easily metabolized by plants and microbes. In these soils, mineralization of organic P can be the major source for orthophosphate. Both plants and microbes encode for phosphatases capable of mineralizing a range of organic P compounds. However, the activity of these enzymes depends on several edaphic factors including P availability, tree species, and microbial communities...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29163453/an-integrated-insight-into-the-relationship-between-soil-microbial-community-and-tobacco-bacterial-wilt-disease
#9
Hongwu Yang, Juan Li, Yunhua Xiao, Yabing Gu, Hongwei Liu, Yili Liang, Xueduan Liu, Jin Hu, Delong Meng, Huaqun Yin
The soil microbial communities play an important role in plant health, however, the relationship between the below-ground microbiome and above-ground plant health remains unclear. To reveal such a relationship, we analyzed soil microbial communities through sequencing of 16S rRNA gene amplicons from 15 different tobacco fields with different levels of wilt disease in the central south part of China. We found that plant health was related to the soil microbial diversity as plants may benefit from the diverse microbial communities...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163397/functionality-of-root-associated-bacteria-along-a-salt-marsh-primary-succession
#10
Miao Wang, Erqin Li, Chen Liu, Alexandre Jousset, Joana F Salles
Plant-associated bacteria are known for their high functional trait diversity, from which many are likely to play a role in primary and secondary succession, facilitating plant establishment in suboptimal soils conditions. Here we used an undisturbed salt marsh chronosequence that represents over 100 years of soil development to assess how the functional traits of plant associated bacteria respond to soil type, plant species and plant compartment. We isolated and characterized 808 bacterial colonies from the rhizosphere soil and root endosphere of two salt marsh plants, Limonium vulgare and Artemisia maritima, along the chronosequence...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163387/a-multiplex-sybr-green-real-time-pcr-assay-for-the-detection-of-three-colistin-resistance-genes-from-cultured-bacteria-feces-and-environment-samples
#11
Jiyun Li, Xiaomin Shi, Wenjuan Yin, Yang Wang, Zhangqi Shen, Shuangyang Ding, Shaolin Wang
The aim of the study was to develop a multiplex assay for rapid detection of mcr-1, mcr-2, and mcr-3, a group of genes of conferring resistance to colistin mediated by plasmid in Enterobacteriaceae. A SYBR Green based real-time PCR assay has been designed to detect the mcr genes, and applied to cultured bacteria, feces and soil samples. All three mcr genes could be detected with a lower limit of 10(2) cultured bacteria. This test was highly specific and sensitive, and generated no false-positive results. The assay was also conclusive when applied to feces and soil samples containing mcr-1-positive Escherichia coli, which could facilitate the screening of mcr genes not only in the bacteria, but also directly from the environment...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163375/isolation-and-characterization-of-pseudomonas-spp-strains-that-efficiently-decompose-sodium-dodecyl-sulfate
#12
Ewa M Furmanczyk, Michal A Kaminski, Grzegorz Spolnik, Maciej Sojka, Witold Danikiewicz, Andrzej Dziembowski, Leszek Lipinski, Adam Sobczak
Due to their particular properties, detergents are widely used in household cleaning products, cosmetics, pharmaceuticals, and in agriculture as adjuvants tailoring the features of pesticides or other crop protection agents. The continuously growing use of these various products means that water soluble detergents have become one of the most problematic groups of pollutants for the aquatic and terrestrial environments. Thus it is important to identify bacteria having the ability to survive in the presence of large quantities of detergent and efficiently decompose it to non-surface active compounds...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29162906/characterisation-of-the-semi-volatile-component-of-dissolved-organic-matter-by-thermal-desorption-proton-transfer-reaction-mass-spectrometry
#13
Dušan Materić, Mike Peacock, Matthew Kent, Sarah Cook, Vincent Gauci, Thomas Röckmann, Rupert Holzinger
Proton Transfer Reaction - Mass Spectrometry (PTR-MS) is a sensitive, soft ionisation method suitable for qualitative and quantitative analysis of volatile and semi-volatile organic vapours. PTR-MS is used for various environmental applications including monitoring of volatile organic compounds (VOCs) emitted from natural and anthropogenic sources, chemical composition measurements of aerosols, etc. Here we apply thermal desorption PTR-MS for the first time to characterise the chemical composition of dissolved organic matter (DOM)...
November 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29161675/a-comparison-of-two-digestion-methods-for-assessing-heavy-metals-level-in-urban-soils-influenced-by-mining-and-industrial-activities
#14
Khaled A M Alsaleh, Helmut Meuser, Adel R A Usman, Mohammad I Al-Wabel, Abdullah S Al-Farraj
A comparison between two digestion methods of hot plate Hossner (total-total) and USEPA method 3051 (total-recoverable) was carried out to suggest a proper method for determining nine heavy metals (Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb and Zn) content of three urban soils affected by mining (Mahd AD'Dahab) or industrial activities (Riyadh and Jubail) at Saudi Arabia. The results showed no significant differences between two digestion methods for Cd, Cu, Pb and Zn in soils affected by mining and for Cr, Cu, Pb and Zn in soils affected by industrial activities...
November 18, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/29161650/how-the-morphological-properties-of-mentha-longifolia-l-huds-may-be-affected-by-geographical-differences
#15
Mohammad Mohammadi, Hossein Ali Asadi-Gharneh
With respect to the benefits of medicinal plants for human health and their use as food, they are extensively being researched and planted in different parts of the world including Iran. Among the most important parameters, which may affect the growth and the yield of medicinal plants is their geographical (ecotype) properties. Accordingly, a research work was proposed to investigate the effects of 10 different regions or ecotypes on the morphological properties of pennyroyal [Mentha longifolia. (L.) Huds.] from the Lamiaceae family in one of the Iranian provinces, namely Ilam...
October 28, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29160699/diversity-and-abundance-of-high-molecular-weight-azaarenes-in-pah-contaminated-environmental-samples
#16
Zhenyu Tian, Joaquim Vila, Hanyan Wang, Wanda Bodnar, Michael D Aitken
Azaarenes are N-heterocyclic polyaromatic pollutants that co-occur with polycyclic aromatic hydrocarbons (PAHs) in contaminated soils. Despite the known toxicity of some high-molecular-weight azaarenes, their diversity, abundance, and fate in contaminated soils remain to be elucidated. We applied high-resolution mass spectrometry and mass-defect filtering to four PAH-contaminated samples from geographically distant sites and detected 232 azaarene congeners distributed in eight homologous series, including alkylated derivatives and two hitherto unknown series...
November 21, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29159702/evaluation-of-levels-sources-and-health-hazards-of-road-dust-associated-toxic-metals-in-jalalabad-and-kabul-cities-afghanistan
#17
Waqar Azeem Jadoon, Wahdatullah Khpalwak, Russel Chrispine Garven Chidya, Sherif Mohamed Mohamed Ali Abdel-Dayem, Kazuhiko Takeda, Masood Arshad Makhdoom, Hiroshi Sakugawa
This study was designed to investigate selected road-dust associated heavy metals, their relations with natural and anthropogenic sources, and potential human and environmental health risks. For this purpose, 42 and 36 road-dusts samples were collected from Jalalabad and Kabul cities (Afghanistan), respectively. The following elements were found in descending concentrations: Mn, Zn, Pb, Ni, Cu, Cr, Co, and Cd in Jalalabad; and Mn, Zn, Ni, Cu, Cr, Pb, Co, and Cd in Kabul. Except for Ni, all the elemental contents were less than the Canadian permissible limits in residential/parkland soils...
November 20, 2017: Archives of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/29158972/short-term-effects-of-co2-leakage-on-the-soil-bacterial-community-in-a-simulated-gas-leakage-scenario
#18
Jing Ma, Wangyuan Zhang, Shaoliang Zhang, Qianlin Zhu, Qiyan Feng, Fu Chen
The technology of carbon dioxide (CO2) capture and storage (CCS) has provided a new option for mitigating global anthropogenic emissions with unique advantages. However, the potential risk of gas leakage from CO2 sequestration and utilization processes has attracted considerable attention. Moreover, leakage might threaten soil ecosystems and thus cannot be ignored. In this study, a simulation experiment of leakage from CO2 geological storage was designed to investigate the short-term effects of different CO2 leakage concentration (from 400 g m(-2) day(-1) to 2,000 g m(-2) day(-1)) on soil bacterial communities...
2017: PeerJ
https://www.readbyqxmd.com/read/29158868/prevalence-of-papular-urticaria-caused-by-flea-bites-and-associated-factors-in-children-1-6%C3%A2-years-of-age-in-bogot%C3%A3-d-c
#19
Evelyne Halpert, Elizabeth Borrero, Milciades Ibañez-Pinilla, Pablo Chaparro, Jorge Molina, Maritza Torres, Elizabeth García
Background: Papular urticaria is a chronic inflammatory disease caused by exposure to arthropod bites. The disease has been reported in children attending medical centers, but the causes as the risk factors associated with the disease have not been established. The objective of this study was to determine the prevalence of papular urticaria caused by flea bite and identify the risk factors in children between 1 to 6 years of age in Bogotá D.C, between March 2009 and June 2011. Methods: A cross-sectional, two-stage, clustered study using random probability sampling and stratified with proportional allocation was carried out in children (1-6 years of age) in educational institutions in Bogotá D...
2017: World Allergy Organization Journal
https://www.readbyqxmd.com/read/29158606/detecting-macroecological-patterns-in-bacterial-communities-across-independent-studies-of-global-soils
#20
Kelly S Ramirez, Christopher G Knight, Mattias de Hollander, Francis Q Brearley, Bede Constantinides, Anne Cotton, Si Creer, Thomas W Crowther, John Davison, Manuel Delgado-Baquerizo, Ellen Dorrepaal, David R Elliott, Graeme Fox, Robert I Griffiths, Chris Hale, Kyle Hartman, Ashley Houlden, David L Jones, Eveline J Krab, Fernando T Maestre, Krista L McGuire, Sylvain Monteux, Caroline H Orr, Wim H van der Putten, Ian S Roberts, David A Robinson, Jennifer D Rocca, Jennifer Rowntree, Klaus Schlaeppi, Matthew Shepherd, Brajesh K Singh, Angela L Straathof, Jennifer M Bhatnagar, Cécile Thion, Marcel G A van der Heijden, Franciska T de Vries
The emergence of high-throughput DNA sequencing methods provides unprecedented opportunities to further unravel bacterial biodiversity and its worldwide role from human health to ecosystem functioning. However, despite the abundance of sequencing studies, combining data from multiple individual studies to address macroecological questions of bacterial diversity remains methodically challenging and plagued with biases. Here, using a machine-learning approach that accounts for differences among studies and complex interactions among taxa, we merge 30 independent bacterial data sets comprising 1,998 soil samples from 21 countries...
November 20, 2017: Nature Microbiology
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