Read by QxMD icon Read


Leslie Mertz
Gene editing and CRISPR (a group of repeated DNA sequences in bacteria) typically target disease-causing mutated genes by eliminating the bad gene altogether, by correcting the problem DNA to restore proper gene functioning, or by modifying a different gene to compensate for the faulty gene's lost function. One research group at Duke University in Durham, North Carolina, however, is using a different strategy to fight one of the most common inherited genetic diseases: Duchenne muscular dystrophy (DMD).
March 2018: IEEE Pulse
Michael P Ryan, Lorin A Neuman-Lee, Susan L Durham, Geoffrey D Smith, Susannah S French
Behavioral fever in reptiles is often considered an adaptive response used to eliminate pathogens, yet empirical data showing the wide-spread use of this response is mixed. This behavioral change can be beneficial by enhancing the host's immune response and increasing the animal's chance of survival, but it can also be detrimental in terms of host energetic requirements and enzymatic performance. Thus, we examined whether captive-bred African house snakes (Lamprophis fuliginosus) employed behavioral fever in response to pathogen stimulus...
April 2018: Journal of Thermal Biology
Davina Gale, Andrew R J Lawson, Karen Howarth, Mikidache Madi, Bradley Durham, Sarah Smalley, John Calaway, Shannon Blais, Greg Jones, James Clark, Peter Dimitrov, Michelle Pugh, Samuel Woodhouse, Michael Epstein, Ana Fernandez-Gonzalez, Alexandra S Whale, Jim F Huggett, Carole A Foy, Gerwyn M Jones, Hadas Raveh-Amit, Karin Schmitt, Alison Devonshire, Emma Green, Tim Forshew, Vincent Plagnol, Nitzan Rosenfeld
INTRODUCTION: Detection and monitoring of circulating tumor DNA (ctDNA) is rapidly becoming a diagnostic, prognostic and predictive tool in cancer patient care. A growing number of gene targets have been identified as diagnostic or actionable, requiring the development of reliable technology that provides analysis of multiple genes in parallel. We have developed the InVision™ liquid biopsy platform which utilizes enhanced TAm-Seq™ (eTAm-Seq™) technology, an amplicon-based next generation sequencing method for the identification of clinically-relevant somatic alterations at low frequency in ctDNA across a panel of 35 cancer-related genes...
2018: PloS One
Antoneicka L Harris, Samantha E Lee, Louis K Dawson, Laura A Marlow, Brandy H Edenfield, William F Durham, Thomas J Flotte, Michael Thompson, Daniel L Small, Aidan J Synnott, Svetomir N Markovic, John A Copland
Patient-derived tumor xenograft (PDTX) mouse models were used to discover new therapies for naïve and drug resistant BRAF V600E -mutant melanoma. Tumor histology, oncogenic protein expression, and antitumor activity were comparable between patient and PDTX-matched models thereby validating PDTXs as predictive preclinical models of therapeutic response in patients. PDTX models responsive and non-responsive to BRAF/MEK standard of care (SOC) therapy were used to identify efficacious combination therapies. One such combination includes a CDK4/6 inhibitor that blocks cell cycle progression...
February 16, 2018: Oncotarget
Angelica Geter, Madeline Y Sutton, Carl Armon, Marcus D Durham, Frank J Palella, Ellen Tedaldi, Rachel Hart, Kate Buchacz
[This corrects the article DOI: 10.1371/journal.pone.0189973.].
2018: PloS One
Benjamin H Durham
The systemic histiocytoses encompass a clinically heterogeneous group of disorders leading to tissue damage secondary to the accumulation and infiltration of pathological cells thought to be derived from the dendritic or monocytic lineages with accompanying inflammation. For decades, whether or not the histiocytoses were inflammatory or neoplastic disorders was unclear, and their cellular origins have long been obscure and heavily debated. However, the rise of the molecular era led to the discovery of recurrent BRAFV600E mutations in approximately 50% of patients with Langerhans cell and non-Langerhans cell histiocytoses, which provided the first convincing evidence that these are indeed histiocytic neoplasms...
March 8, 2018: Seminars in Cell & Developmental Biology
Kristina K Durham, Kevin M Chathely, Bernardo L Trigatti
The cardioprotective lipoprotein HDL prevents myocardial infarction , and cardiomyocyte death due to ischemia/reperfusion injury .  The scavenger receptor class B, type 1 (SR-B1) is a high affinity HDL receptor and has been shown to mediate HDL dependent lipid transport as well as signaling in a variety of different cell types.  The contribution of SR-B1 in cardiomyocytes to the protective effects of HDL on cardiomyocyte survival following ischemia has not yet been studied.  Here we use a model of simulated ischemia (oxygen and glucose deprivation; OGD), to assess the mechanistic involvement of SR-B1, PI3K, and AKT in HDL mediated protection of cardiomyocytes from cell death...
March 9, 2018: Biochemical Journal
J L Hawkins, N J Moore, D Miley, P L Durham
The pathology of migraine, a common neurological disease, involves sensitization and activation of trigeminal nociceptive neurons to promote hyperalgesia and allodynia during an attack. Migraineurs often exhibit characteristics of a hyperexcitable or hypervigilant nervous system. One of the primary reported risk factors for development of a hyperexcitable trigeminal system is chronic, unmanaged stress and anxiety. While primary traumatic stress is a commonly cited risk factor for many pain conditions, exposure to secondary traumatic stress early in life is also thought to be a contributing risk factor...
March 6, 2018: Brain Research
Yunxia O'Malley, Pavana G Rotti, Ian M Thornell, Oriana G Vanegas Calderón, Christopher Febres-Aldana, Katelin Durham, Jianrong Yao, Xiaopeng Li, Zheng Zhu, Andrew W Norris, Joseph Zabner, John F Engelhardt, Aliye Uc
Pancreatic ductular epithelial cells comprise the majority of duct cells in pancreas, control cystic fibrosis transmembrane conductance regulator (CFTR)-dependent bicarbonate [HCO3 - ] secretion, but are difficult to grow as a polarized monolayer. Using NIH-3T3-J2 fibroblast feeder cells and a Rho-associated kinase inhibitor, we produced well-differentiated and polarized porcine pancreatic ductular epithelial cells. Cells grown on semipermeable filters at air-liquid interface (ALI) developed typical epithelial cell morphology and stable transepithelial resistance (TER), expressed epithelial cell markers (zona occludens-1 and beta catenin), duct cell markers (SOX-9 and CFTR), but no acinar (amylase) or islet cell (chromogranin) markers...
March 8, 2018: Journal of Applied Physiology
Andrew L Durham, Mei Y Speer, Marta Scatena, Cecilia M Giachelli, Catherine M Shanahan
Vascular calcification is associated with a significant increase in all-cause mortality and atherosclerotic plaque rupture. Calcification has been determined to be an active process driven in part by vascular smooth muscle cell (VSMC) transdifferentiation within the vascular wall. Historically, VSMC phenotype switching has been viewed as binary, with the cells able to adopt a physiological contractile phenotype or an alternate 'synthetic' phenotype in response to injury. More recent work, including lineage tracing has however revealed that VSMCs are able to adopt a number of phenotypes, including calcific (osteogenic, chondrocytic, and osteoclastic), adipogenic, and macrophagic phenotypes...
March 15, 2018: Cardiovascular Research
Ralph Mösges, Claus Bachert, Petr Panzner, Moises A Calderon, Ludo Haazen, Sabine Pirotton, Nathalie Wathelet, Stephen R Durham, Marie-Alix Bonny, Thierry Legon, Remy von Frenckell, Oliver Pfaar, Mohamed H Shamji
BACKGROUND: Immunotherapy with peptide hydrolysates from Lolium perenne (LPP) is an alternative treatment for seasonal allergic rhinitis with or without asthma. The aim of this study was to assess the clinical efficacy and safety of a cumulative dose of 170 μg LPP administered subcutaneously over 3 weeks. METHODS: In a randomized, double blind, placebo-controlled trial, 554 adults with grass pollen rhinoconjunctivitis were randomized (1:2 ratio) to receive 8 subcutaneous injections of placebo or 170 μg LPP administered in increasing doses in 4 visits over 3 weeks...
March 7, 2018: Allergy
Jordan L Hawkins, Paul L Durham
AIMS: To test a commercially available enriched chicken bone broth (ECBB) product for its potential anti-inflammatory properties and to evaluate its ability to reduce nociception and expression of protein kinase A (PKA) in a clinically relevant model of temporomandibular disorder (TMD) caused by prolonged jaw opening in rats. METHODS: The potential of the ECBB and of a homemade broth was investigated using the Folin-Ciocalteu reagent and percent inhibition of cyclooxygenase-2 (COX-2) activity, which was determined using a commercially available kit...
March 6, 2018: Journal of Oral & Facial Pain and Headache
Hope E Arnold, Katherine J Baxter, Heather L Short, Curtis Travers, Amina Bhatia, Megan M Durham, Mehul V Raval
BACKGROUND: Our goal is to determine short- and long-term outcomes of simple gastroschisis (SG) and complicated gastroschisis (CG) patients including quality of life (QoL) measures, surgical reoperation rates, and residual gastrointestinal symptom burden. MATERIALS AND METHODS: Retrospective chart review of patients who underwent surgical repair of gastroschisis between January 1, 2009, and December 31, 2012, was performed at a quaternary children's hospital. Parent telephone surveys were conducted to collect information on subsequent operations and current health status as well as to assess QoL using two validated tools...
April 2018: Journal of Surgical Research
C Aidala, Y Akiba, M Alfred, V Andrieux, K Aoki, N Apadula, H Asano, C Ayuso, B Azmoun, V Babintsev, A Bagoly, N S Bandara, K N Barish, S Bathe, A Bazilevsky, M Beaumier, R Belmont, A Berdnikov, Y Berdnikov, D S Blau, M Boer, J S Bok, M L Brooks, J Bryslawskyj, V Bumazhnov, C Butler, S Campbell, V Canoa Roman, R Cervantes, C Y Chi, M Chiu, I J Choi, J B Choi, Z Citron, M Connors, N Cronin, M Csanád, T Csörgő, T W Danley, M S Daugherity, G David, K DeBlasio, K Dehmelt, A Denisov, A Deshpande, E J Desmond, A Dion, D Dixit, J H Do, A Drees, K A Drees, M Dumancic, J M Durham, A Durum, T Elder, A Enokizono, H En'yo, S Esumi, B Fadem, W Fan, N Feege, D E Fields, M Finger, M Finger, S L Fokin, J E Frantz, A Franz, A D Frawley, Y Fukuda, C Gal, P Gallus, P Garg, H Ge, F Giordano, Y Goto, N Grau, S V Greene, M Grosse Perdekamp, T Gunji, H Guragain, T Hachiya, J S Haggerty, K I Hahn, H Hamagaki, H F Hamilton, S Y Han, J Hanks, S Hasegawa, T O S Haseler, X He, T K Hemmick, J C Hill, K Hill, A Hodges, R S Hollis, K Homma, B Hong, T Hoshino, N Hotvedt, J Huang, S Huang, K Imai, J Imrek, M Inaba, A Iordanova, D Isenhower, Y Ito, D Ivanishchev, B V Jacak, M Jezghani, Z Ji, X Jiang, B M Johnson, V Jorjadze, D Jouan, D S Jumper, J H Kang, D Kapukchyan, S Karthas, D Kawall, A V Kazantsev, V Khachatryan, A Khanzadeev, C Kim, D J Kim, E-J Kim, M Kim, M H Kim, D Kincses, E Kistenev, J Klatsky, P Kline, T Koblesky, D Kotov, S Kudo, K Kurita, Y Kwon, J G Lajoie, E O Lallow, A Lebedev, S Lee, S H Lee, M J Leitch, Y H Leung, N A Lewis, X Li, S H Lim, L D Liu, M X Liu, V-R Loggins, S Lökös, K Lovasz, D Lynch, T Majoros, Y I Makdisi, M Makek, M Malaev, V I Manko, E Mannel, H Masuda, M McCumber, P L McGaughey, D McGlinchey, C McKinney, M Mendoza, W J Metzger, A C Mignerey, D E Mihalik, A Milov, D K Mishra, J T Mitchell, G Mitsuka, S Miyasaka, S Mizuno, P Montuenga, T Moon, D P Morrison, S I M Morrow, T Murakami, J Murata, K Nagai, K Nagashima, T Nagashima, J L Nagle, M I Nagy, I Nakagawa, H Nakagomi, K Nakano, C Nattrass, T Niida, R Nouicer, T Novák, N Novitzky, R Novotny, A S Nyanin, E O'Brien, C A Ogilvie, J D Orjuela Koop, J D Osborn, A Oskarsson, G J Ottino, K Ozawa, V Pantuev, V Papavassiliou, J S Park, S Park, S F Pate, M Patel, W Peng, D V Perepelitsa, G D N Perera, D Yu Peressounko, C E PerezLara, J Perry, R Petti, M Phipps, C Pinkenburg, R P Pisani, A Pun, M L Purschke, P V Radzevich, K F Read, D Reynolds, V Riabov, Y Riabov, D Richford, T Rinn, S D Rolnick, M Rosati, Z Rowan, J Runchey, A S Safonov, T Sakaguchi, H Sako, V Samsonov, M Sarsour, K Sato, S Sato, B Schaefer, B K Schmoll, K Sedgwick, R Seidl, A Sen, R Seto, A Sexton, D Sharma, I Shein, T-A Shibata, K Shigaki, M Shimomura, T Shioya, P Shukla, A Sickles, C L Silva, D Silvermyr, B K Singh, C P Singh, V Singh, M J Skoby, M Slunečka, K L Smith, M Snowball, R A Soltz, W E Sondheim, S P Sorensen, I V Sourikova, P W Stankus, S P Stoll, T Sugitate, A Sukhanov, T Sumita, J Sun, S Syed, J Sziklai, A Takeda, K Tanida, M J Tannenbaum, S Tarafdar, A Taranenko, G Tarnai, R Tieulent, A Timilsina, T Todoroki, M Tomášek, C L Towell, R S Towell, I Tserruya, Y Ueda, B Ujvari, H W van Hecke, S Vazquez-Carson, J Velkovska, M Virius, V Vrba, N Vukman, X R Wang, Z Wang, Y Watanabe, Y S Watanabe, C P Wong, C L Woody, C Xu, Q Xu, L Xue, S Yalcin, Y L Yamaguchi, H Yamamoto, A Yanovich, P Yin, J H Yoo, I Yoon, H Yu, I E Yushmanov, W A Zajc, A Zelenski, S Zharko, L Zou
Recently, multiparticle-correlation measurements of relativistic p/d/^{3}He+Au, p+Pb, and even p+p collisions show surprising collective signatures. Here, we present beam-energy-scan measurements of two-, four-, and six-particle angular correlations in d+Au collisions at sqrt[s_{NN}]=200, 62.4, 39, and 19.6 GeV. We also present measurements of two- and four-particle angular correlations in p+Au collisions at sqrt[s_{NN}]=200  GeV. We find the four-particle cumulant to be real valued for d+Au collisions at all four energies...
February 9, 2018: Physical Review Letters
Carrie L Cormack, Elizabeth Jensen, Catherine O Durham, Gigi Smith, Bonnie Dumas
BACKGROUND: The 360 Degree Evaluation Model is one means to provide a comprehensive view of clinical competency and readiness for progression in an online nursing program. OBJECTIVES: This pilot project aimed to evaluate the effectiveness of implementing a 360 Degree Evaluation of clinical competency of graduate advanced practice nursing students. DESIGN: The 360 Degree Evaluation, adapted from corporate industry, encompasses assessment of student knowledge, skills, behaviors and attitudes and validates student's progression from novice to competent...
February 5, 2018: Nurse Education Today
Ankush Bhatia, Gary Ulaner, Raajit Rampal, David M Hyman, Omar Abdel-Wahab, Benjamin H Durham, Ahmet Dogan, Neval Ozkaya, Mario E Lacouture, Julio Hajdenberg, Chezi Ganzel, Eli L Diamond
No abstract text is available yet for this article.
February 22, 2018: Haematologica
Sarah Rhea, Arlene C Seña, Alison Hilton, Christopher B Hurt, David Wohl, Aaron Fleischauer
Guidance about integration of comprehensive hepatitis C virus (HCV)-related services in sexually transmitted disease (STD) clinics is limited. We evaluated a federally-funded HCV testing and linkage-to-care program at an STD clinic in Durham County, North Carolina. During December 10, 2012-March 31, 2015, the program tested 733 patients for HCV who reported ≥1 HCV risk factor; 81 (11%) were HCV-infected (i.e., HCV antibody-positive and HCV ribonucleic acid-positive). Fifty-one infected patients (63%) were linked to care...
October 19, 2017: Sexually Transmitted Diseases
Ted G Graber, Brandy L Rawls, Bing Tian, William J Durham, Camille R Brightwell, Allan R Brasier, Blake B Rasmussen, Christopher S Fry
Due to immunosenescence, older adults are particularly susceptible to lung-based viral infections, with increased severity of symptoms in those with underlying chronic lung disease. Repeated respiratory viral infections produce lung maladaptations, accelerating pulmonary dysfunction. Toll like 3 receptor (TLR3) is a membrane protein that senses exogenous double-stranded RNA to activate the innate immune response to a viral infection. Polyinosinic-polycytidylic acid [poly(I:C)] mimics double stranded RNA and has been shown to activate TLR3...
February 13, 2018: Experimental Gerontology
Priscilla Savopoulos, Annukka K Lindell
Over 100 years ago Lombroso [(1876/2006). Criminal man. Durham: Duke University Press] proposed a biological basis for criminality. Based on inspection of criminals' skulls he theorized that an imbalance of the cerebral hemispheres was amongst 18 distinguishing features of the criminal brain. Specifically, criminals were less lateralized than noncriminals. As the advent of neuroscientific techniques makes more fine-grained inspection of differences in brain structure and function possible, we review criminals' and noncriminals' structural, functional, and behavioural lateralization to evaluate the merits of Lombroso's thesis and investigate the evidence for the biological underpinning of criminal behaviour...
February 15, 2018: Laterality
Harman S Parhar, Donald W Anderson, Arif S Janjua, J Scott Durham, Eitan Prisman
Objectives There are well-established outcome disparities among different demographic groups with head and neck squamous cell carcinoma (HNSCC). We aimed to investigate the potential contribution of patient choice of nonsurgical treatment to these disparities by estimating the rate of this phenomenon, identifying its predictors, and estimating the effect on cancer-specific survival. Study Design Retrospective nationwide analysis. Settings Surveillance, Epidemiology, and End Results Database (2004-2014). Subjects and Methods Patients with HNSCC, who were recommended for primary surgery, were included...
February 1, 2018: Otolaryngology—Head and Neck Surgery
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"