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Jiqing Yang, Xiaotong Hu, Jia Xu, Xin Liu, Li Yang
A novel capillary electrophoresis-integrated immobilized enzyme reactor (CE-integrated IMER) is developed using single-step in-situ acetylcholinesterase (AChE)-mediated alginate hydrogelation and enzyme encapsulation. Alginate hydrogelation with "egg-box" structure is triggered inside a capillary with releasing of Ca2+ by changing the pH of the sol solution, which is accomplished in-situ by AChE-catalyzed hydrolysis reaction of acetylthiocholine to produce acetic acid. AChE and any other enzyme initially contained in the sol solution [e...
February 22, 2018: Analytical Chemistry
Attawut Chaibunruang, Kritsada Singha, Hataichanok Srivorakun, Goonnapa Fucharoen, Supan Fucharoen
Hb New York or Hb Kaohsiung [β113(G15)Val→Glu (GTG>GAG), HBB: c.341T>A] has been considered a rare β hemoglobin (Hb) variant found originally in an Iranian woman and later in diverse populations but its genetic origin has not been elucidated. Here we report molecular and hematological descriptions of this variant found in the Thai population. Among 5643 subjects referred for hemoglobinopathy investigation during January 2015 to September 2017, 183 (3.2%) were found to carry several Hb variants, including β chain variants (n = 135, 2...
February 21, 2018: Hemoglobin
Dingsheng Wang, Pingyu Xin, Jia Li, Yu Xiong, Xi Wu, Juncai Dong, Wenxing Chen, Yu Wang, Lin Gu, Jun Luo, Hongpan Rong, Chen Chen, Qing Peng, Yadong Li
Supported metal catalysts are one of the foundations of modern chemical industry. The active species in such catalysts are always elusive to identify, and in the meanwhile large quantities of inert species can cause significant waste. Herein, using a stoichiometrically precise synthetic method, we prepare atomically dispersed palladium-cerium oxide (Pd1/CeO2) and hexapalladium cluster-cerium oxide (Pd6/CeO2), as confirmed by spherical-aberration-corrected transmission electron microscopy and X-ray absorption fine structure spectroscopy...
February 21, 2018: Angewandte Chemie
Xin Liang, Lei Shen
In2 O3 (ZnO)k natural superlattices (where k is an integer), consisting of relatively earth abundant and non-toxic elements with coarsening-resistant nanostructures, are environmentally friendly materials with the potential for high temperature thermoelectric applications. Herein, we report our investigation of the high temperature thermoelectric properties of the In2 O3 (ZnO)4 superlattice bulk polycrystals that were singly and dually doped with Al and Ce. Transport property measurements revealed that Al and Ce did not only enter the ZnO blocks but also modified the InO2 single atomic layers...
February 20, 2018: Nanoscale
Chad T Palumbo, Dominik P Halter, Vamsee K Voora, Guo P Chen, Alan K Chan, Megan E Fieser, Joseph W Ziller, Wolfgang Hieringer, Filipp Furche, Karsten Meyer, William J Evans
The synthesis of 4fn Ln3+ complexes of the tris(aryloxide) mesitylene ligand, ((Ad,Me ArO)3 mes)3- , with Ln = La, Ce, Pr, Sm, and Yb, and their reduction with potassium have revealed that this ligand system can be redox active with some metals. Protonolysis of [Ln(N(SiMe3 )2 )3 ] (Ln = La, Ce, Pr, Sm, Yb) with the tris(phenol) (Ad,Me ArOH)3 mes yielded the Ln3+ complexes [((Ad,Me ArO)3 mes)Ln] (Ln = La, Ce, Pr, Sm, Yb), 1-Ln. Single electron reduction of each 4fn complex, 1-Ln, using potassium yielded the reduced products, [K(2...
February 19, 2018: Inorganic Chemistry
Giovanni Marchegiani, Stefano Andrianello, Alex Borin, Chiara Dal Borgo, Giampaolo Perri, Tommaso Pollini, Giorgia Romanò, Mirko D'Onofrio, Armando Gabbrielli, Aldo Scarpa, Giuseppe Malleo, Claudio Bassi, Roberto Salvia
BACKGROUND: Mural nodules (MNs) have a predominant role in the 2016 revision of the international guidelines on intraductal papillary mucinous neoplasms (IPMN) of the pancreas. The aim of this study was to evaluate MNs as predictors of invasive cancer (iCa) or high-grade dysplasia (HGD) in IPMNs and to investigate the role of MN size in risk prediction. METHODS: A PRISMA-compliant systematic review of the literature and meta-analysis on selected studies were conducted...
February 14, 2018: Surgery
Fiyinfoluwa A Adesioye, Thulani P Makhalanyane, Surendra Vikram, Bryan T Sewell, Wolf-Dieter Schubert, Don A Cowan
A hot desert hypolith metagenomic DNA sequence dataset was screened in-silico for genes annotated as acetyl xylan esterases (AcXEs). One of the genes identified encoded a ∼36 kDa protein (Axe1 NaM1 ). The synthesised gene was cloned, expressed and the resulting protein, purified. NaM1 was optimally active at pH 8.5 and 30°C, and functionally stable at salt concentrations up to 5 M. The specific activity and catalytic efficiency were 488.9 Umg -1 and 3.26x10 6 M -1 s -1 , respectively. The crystal structure of wild type NaM1 was solved at a resolution of 2...
February 16, 2018: Applied and Environmental Microbiology
Yuanyuan Xu, Yu-Qing Jiang, Ce Li, Mindi He, W George Rusyniak, Naga Annamdevula, Juan Ochoa, Silas J Leavesley, Jiangping Xu, Thomas C Rich, Mike T Lin, Xiang-Ming Zha
Acid-sensing ion channels (ASICs) are the major proton receptor in the brain and a key mediator of acidosis-induced neuronal injuries in disease. Most of published data on ASIC function came from studies performed in mice, and relatively little is known about potential differences between human and mouse ASICs (hASIC and mASIC, respectively). This information is critical for us to better interpret the functional importance of ASICs in human disease. Here, we examined the expression of ASICs in acutely resected human cortical tissue...
February 15, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Oliver Reuthebuch, Luca Koechlin, Ulrich Schurr, Martin Grapow, Jens Fassl, Friedrich S Eckstein
OBJECTIVE: To assess the clinical implementation and report preliminary results of a novel technique called the Ozaki procedure for stentless aortic valve replacement through reconstruction of the valve leaflets from autologous pericardium. METHOD: Between September 2015 and May 2017 30 patients (20 males, mean ± standard deviation age 66.83 ± 10.55 years) suffering from aortic stenosis (AS, n = 7), aortic regurgitation (AR, n = 12), or a combination of both (AS/AR, n = 11) were assigned for an Ozaki procedure...
February 14, 2018: Swiss Medical Weekly
Connor Puett, Christina Inscoe, Yueh Z Lee, Otto Zhou, Jianping Lu
Stationary digital breast tomosynthesis (sDBT) is an emerging technology in which the single rotating x-ray tube is replaced by a fixed array of multiple carbon nanotube-enabled sources, providing a higher spatial and temporal resolution. As such, sDBT offers a promising platform for contrast-enhanced (CE) imaging. However, given the minimal enhancement above background with standard operational tube settings and iodine dosing, CE breast imaging requires additional acquisition steps to isolate the iodine signal, using either temporal or dual energy subtraction (TS or DES) protocols...
January 2018: Journal of Medical Imaging
C X Ma, G Y Zheng
Objective: To construct lentiviral-mediated EphA2 overexpression vectors, transfect them into human lens epithelial cells (HLE-B3) in vitro, and investigate the effect of EphA2 gene overexpression on the proliferation and apoptosis of HLE-B3 exposed to high-concentration dexamethasone. Methods: Experimental Study. The pCDH-CMV- MCS-EF1-RFP plasmid was set up by the digestion of NOTⅠand XbaⅠ double restriction enzyme and ligation of CE ligase, and then the plasmid was transformed into DH10B cells. Seven clons were picked for enzymatic digestion and the clons with correct results were chosen for sequencing...
February 11, 2018: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
Anne Bergougnoux, Valeria D'Argenio, Stefanie Sollfrank, Fanny Verneau, Antonella Telese, Irene Postiglione, Karl J Lackner, Mireille Claustres, Giuseppe Castaldo, Heidi Rossman, Francesco Salvatore, Caroline Raynal
BACKGROUND: Many European laboratories offer molecular genetic analysis of the CFTR gene using a wide range of methods to identify mutations causative of cystic fibrosis (CF) and CFTR-related disorders (CFTR-RDs). Next-generation sequencing (NGS) strategies are widely used in diagnostic practice, and CE marking is now required for most in vitro diagnostic (IVD) tests in Europe. The aim of this multicenter study, which involved three European laboratories specialized in CF molecular analysis, was to evaluate the performance of Multiplicom's CFTR MASTR Dx kit to obtain CE-IVD certification...
February 10, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
Shingo Ohira, Kentaro Wada, Takero Hirata, Naoyuki Kanayama, Toshiki Ikawa, Tsukasa Karino, Yuya Nitta, Masaru Isono, Yoshihiro Ueda, Masayoshi Miyazaki, Masahiko Koizumi, Teruki Teshima
BACKGROUND AND PURPOSE: The accurate delineation of pancreatic tumor with respiratory motion is challenging. This study demonstrates the application of contrast-enhanced four-dimensional dual-energy computed tomography (CE-4D-DECT) for tumor delineation and assesses the objective and subjective image quality. MATERIAL AND METHODS: Twelve patients underwent CE-4D-DECT, and quantitative spectral analysis was performed on the resulting virtual monochromatic images (VMI) to determine the optimal VMI (O-VMI) with the highest contrast-to-noise ratio (CNR)...
February 5, 2018: Radiotherapy and Oncology: Journal of the European Society for Therapeutic Radiology and Oncology
Joshua G Cronmiller, Bram F Noble
Cumulative effects (CE) monitoring is foundational to emerging regional and watershed CE management frameworks, yet monitoring is often poorly integrated with cumulative effects management and decision-making processes. The challenges are largely institutional and organizational, more so than scientific or technical. Calls for improved integration of monitoring with CE management and decision-making are not new, but there has been limited research on how best to integrate environmental monitoring programs to ensure credible CE science, and deliver results that respond to the more immediate questions and needs of regulatory decision makers...
February 8, 2018: Integrated Environmental Assessment and Management
Laurence Devesse, David Ballard, Lucinda Davenport, Immy Riethorst, Gabriella Mason-Buck, Denise Syndercombe Court
By using sequencing technology to genotype loci of forensic interest it is possible to simultaneously target autosomal, X and Y STRs as well as identity, ancestry and phenotypic informative SNPs, resulting in a breadth of data obtained from a single run that is considerable when compared to that generated with standard technologies. It is important however that this information aligns with the genotype data currently obtained using commercially available kits for CE-based investigations such that results are compatible with existing databases and hence can be of use to the forensic community...
November 4, 2017: Forensic Science International. Genetics
Martina Kristofova, Yogesh D Aher, Marija Ilic, Bojana Radoman, Predrag Kalaba, Vladimir Dragacevic, Nilima Y Aher, Johann Leban, Volker Korz, Lisa Zanon, Winfried Neuhaus, Marcus Wieder, Thierry Langer, Ernst Urban, Harald H Sitte, Harald Hoeger, Gert Lubec, Jana Aradska
Dopamine reuptake inhibitors have been shown to improve cognitive parameters in various tasks and animal models. We recently reported a series of modafinil analogues, of which the most promising, 5-((benzhydrylsulfinyl)methyl) thiazole (CE-123), was selected for further development. The present study aims to characterize pharmacological properties of CE-123 and to investigate the potential to enhance memory performance in a rat model. In vitro transporter assays were performed in cells expressing human transporters...
February 1, 2018: Behavioural Brain Research
Seungeun Lee, Min Sun Lee, Jun Yeon Won, Jae Sung Lee
In this study, we measured the performance of a newly developed Hamamatsu Photonics R13478 photomultiplier tube (PMT) and compared the results with those for an existing R9800 PMT. In R13478, an accelerating electrode is placed between the focusing electrode and first dynode for time resolution improvement through reduced transit time jitter. We investigated the time resolution dependence on the supply voltage and time pickoff method for R13478 and R9800 PMTs, each coupled with a 2.9 × 2.9 × 20 mm3 fast LGSO:Ce (0...
February 6, 2018: Physics in Medicine and Biology
Clayton Cozzan, Guillaume Lheureux, Nicholas O'Dea, Emily E Levin, Jake Graser, Taylor D Sparks, Shuji Nakamura, Steven P DenBaars, Claude Weisbuch, Ram Seshadri
Solid-state lighting using laser diodes is an exciting new development that requires new phosphor geometries to handle the greater light fluxes involved. The greater flux from the source results in more conversion and therefore more conversion loss in the phosphor, which generates self-heating, surpassing the stability of current encapsulation strategies used for light-emitting diodes, usually based on silicones. Here, we present a rapid method using spark plasma sintering (SPS) for preparing ceramic phosphor composites of the canonical yellow-emitting phosphor Ce-doped yttrium aluminum garnet (Ce:YAG) combined with a chemically compatible and thermally stable oxide, α-Al2O3...
February 5, 2018: ACS Applied Materials & Interfaces
E M Harding-Esch, E C Cousins, S-L C Chow, L T Phillips, C L Hall, N Cooper, S S Fuller, A V Nori, R Patel, S Thomas-William, G Whitlock, S J E Edwards, M Green, J Clarkson, B Arlett, J K Dunbar, C M Lowndes, S T Sadiq
BACKGROUND: Rapid Point-Of-Care Tests for Chlamydia trachomatis (CT) may reduce onward transmission and reproductive sexual health (RSH) sequelae by reducing turnaround times between diagnosis and treatment. The io® single module system (Atlas Genetics Ltd.) runs clinical samples through a nucleic acid amplification test (NAAT)-based CT cartridge, delivering results in 30min. METHODS: Prospective diagnostic accuracy study of the io® CT-assay in four UK Genito-Urinary Medicine (GUM)/RSH clinics on additional-to-routine self-collected vulvovaginal swabs...
January 10, 2018: EBioMedicine
Yaxing Wang, Huangjie Lu, Xing Dai, Tao Duan, Xiaojing Bai, Yawen Cai, Xuemiao Yin, Lanhua Chen, Juan Diwu, Shiyu Du, Ruhong Zhou, Zhifang Chai, Thomas E Albrecht-Schmitt, Ning Liu, Shuao Wang
The coexistence of radioactive contaminants (e.g., thorium, uranium, and their daughters) in rare earth minerals introduces significant environmental, economic, and technological hurdles in modern rare earth production. Efficient, low cost, and green decontamination strategies are therefore desired to ameliorate this problem. We report here a single-step and quantitative decontamination strategy of thorium from rare earths based on a unique periodic trend in the formation of crystalline selenite compounds across the lanthanide series, where Ce(III) is fully oxidized in situ to Ce(IV)...
February 2, 2018: Inorganic Chemistry
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