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https://www.readbyqxmd.com/read/27922184/europium-iii-and-uranium-vi-complexation-by-natural-organic-matter-nom-effect-of-source
#1
Ralf Kautenburger, Jonas M Sander, Christina Hein
For the safe long-term storage of high level radioactive waste (HLW), detailed information about geo-chemical behavior of radioactive and toxic metal ions under environmental conditions is important. Natural organic matter (NOM) can play a crucial role in the immobilization or mobilization of these metal ions due to its complexation and colloid formation tendency. In this study, the complexation of europium (as chemical homologue of trivalent actinides like americium) and uranium (as main component of HLW) by ten humic acids (HA) from different sources and Suwannee NOM river extract has been analyzed...
December 6, 2016: Electrophoresis
https://www.readbyqxmd.com/read/27910953/three-millennia-of-heavy-rainfalls-in-western-mediterranean-frequency-seasonality-and-atmospheric-drivers
#2
J P Corella, B L Valero-Garcés, S M Vicente-Serrano, A Brauer, G Benito
Documenting subdecadal-scale heavy rainfall (HR) variability over several millennia can rarely be accomplished due to the paucity of high resolution, homogeneous and continuous proxy records. Here, using a unique, seasonally resolved lake record from southern Europe, we quantify temporal changes in extreme HR events for the last 2,800 years in this region and their correlation with negative phases of the Mediterranean Oscillation (MO). Notably, scarce HR dominated by a persistent positive MO mode characterizes the so-called Migration period (CE 370-670)...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910594/quantitative-low-energy-ion-beam-characterization-by-beam-profiling-and-imaging-via-scintillation-screens
#3
S Germer, F Pietag, J Polak, T Arnold
This study presents the imaging and characterization of low-current ion beams in the neutralized state monitored via single crystal YAG:Ce (Y3Al5O12) scintillators. To validate the presented beam diagnostic tool, Faraday cup measurements and test etchings were performed. Argon ions with a typical energy of 1.0 keV were emitted from an inductively coupled radio-frequency (13.56 MHz) ion beam source with total currents of some mA. Different beam properties, such as, lateral ion current density, beam divergence angle, and current density in pulsed ion beams have been studied to obtain information about the spatial beam profile and the material removal rate distribution...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27900511/non-doped-deep-blue-and-doped-white-electroluminescence-devices-based-on-phenanthroimidazole-derivative
#4
Shuo Chen, Yukun Wu, Shoucheng Hu, Yi Zhao, Daining Fang
A novel deep-blue emitter PhImPOTD based on phenathroimidazole was synthesized, which is incorporated by an electron-donating dibenzothiophene unit and electron-withdrawing phenanthroimidazole and diphenylphosphine oxide moieties. Furthermore, the weak π-π stacking and intermolecular aggregation render the photoluminescence quantum yield is as high as 0.34 in the solid state. Non-doped organic light emitting diodes (OLEDs) based on PhImPOTD emitter exhibits a low turn-on voltage of 3.6 V, a favorable efficiency of 1...
November 29, 2016: Journal of Fluorescence
https://www.readbyqxmd.com/read/27900431/interspecies-comparison-of-peptide-substrate-reporter-metabolism-using-compartment-based-modeling
#5
Allison J Tierney, Nhat Pham, Kunwei Yang, Brooks K Emerick, Michelle L Kovarik
Peptide substrate reporters are fluorescently labeled peptides that can be acted upon by one or more enzymes of interest. Peptide substrates are readily synthesized and more easily separated than full-length protein substrates; however, they are often more rapidly degraded by peptidases. As a result, peptide reporters must be made resistant to proteolysis in order to study enzymes in intact cells and lysates. This is typically achieved by optimizing the reporter sequence in a single cell type or model organism, but studies of reporter stability in a variety of organisms are needed to establish the robustness and broader utility of these molecular tools...
November 29, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27898666/algorithm-optimization-in-methylation-detection-with-multiple-rt-qpcr
#6
Lele Song, Yuemin Li, Jia Jia, Guangpeng Zhou, Jianming Wang, Qian Kang, Peng Jin, Jianqiu Sheng, Guoxiang Cai, Sanjun Cai, Xiaoliang Han
Epigenetic markers based on differential methylation of DNA sequences are used in cancer screening and diagnostics. Detection of abnormal methylation at specific loci by real-time quantitative polymerase chain reaction (RT-qPCR) has been developed to enable high-throughput cancer screening. For tests that combine the results of multiple PCR replicates into a single reportable result, both individual PCR cutoff and weighting of the individual PCR result are essential to test outcome. In this opportunistic screening study, we tested samples from 1133 patients using the triplicate Epi proColon assay with various algorithms and compared it with the newly developed single replicate SensiColon assay that measures methylation status of the same SEPT9 gene sequence...
2016: PloS One
https://www.readbyqxmd.com/read/27884429/development-of-separation-methods-for-the-chiral-resolution-of-hexahelicenes
#7
Jan Storch, Květa Kalíková, Eva Tesařová, Vítězslav Maier, Jan Vacek
In this short communication we report optimized procedures for the chiral separation of non-charged [6]helicene (1) and cationic derivative 1-butyl-3-(2-methyl[6]helicenyl)-imidazolium bromide (2) using high-performance liquid chromatography (HPLC) and supercritical fluid chromatography (SFC) methods. The possibility of using capillary electrophoresis (CE) was also tested. The satisfactory results were obtained with SFC, where the highly selective resolution of four enantiopure 1 and 2 helicenes was achieved in a single run within 5min...
December 9, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27873314/characterization-of-a-novel-cellobiose-2-epimerase-from-thermophilic-caldicellulosiruptor-obsidiansis-for-lactulose-production
#8
Qiuming Chen, Roman Levin, Wenli Zhang, Tao Zhang, Bo Jiang, Timo Stressler, Lutz Fischer, Wanmeng Mu
BACKGROUND: Lactulose, a bioactive lactose derivative, has been widely used in food and pharmaceutical industries. Isomerization of lactose to lactulose by cellobiose 2-epimerase (CE) has recently attracted increasing attention, since CE produces lactulose with high yield from lactose as a single substrate. In this study, a new lactulose-producing CE from Caldicellulosiruptor obsidiansis was extensively characterized. RESULTS: The recombinant enzyme exhibited maximal activity at pH 7...
November 21, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27867109/towards-long-lasting-zirconia-based-composites-for-dental-implants-transformation-induced-plasticity-and-its-consequence-on-ceramic-reliability
#9
Helen Reveron, Marta Fornabaio, Paola Palmero, Tobias Fürderer, Erik Adolfsson, Vanni Lughi, Alois Bonifacio, Valter Sergo, Laura Montanaro, Jérôme Chevalier
: Zirconia-based composites were developed through an innovative processing route able to tune compositional and microstructural features very precisely. Fully-dense ceria-stabilized zirconia ceramics (84vol% Ce-TZP) containing equiaxed alumina (8vol%Al2O3) and elongated strontium hexa-aluminate (8vol% SrAl12O19) second phases were obtained by conventional sintering. This work deals with the effect of the zirconia stabilization degree (CeO2 in the range 10.0-11.5mol%) on the transformability and mechanical properties of Ce-TZP-Al2O3-SrAl12O19 materials...
November 17, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27865495/assessment-of-multifunctional-activity-of-bioactive-peptides-derived-from-fermented-milk-by-specific-lactobacillus-plantarum-strains
#10
J E Aguilar-Toalá, L Santiago-López, C M Peres, C Peres, H S Garcia, B Vallejo-Cordoba, A F González-Córdova, A Hernández-Mendoza
Milk-derived bioactive peptides with a single activity (e.g., antioxidant, immunomodulatory, or antimicrobial) have been previously well documented; however, few studies describe multifunctional bioactive peptides, which may be preferred over single-activity peptides, as they can simultaneously trigger, modulate, or inhibit multiple physiological pathways. Hence, the aim of this study was to assess the anti-inflammatory, antihemolytic, antioxidant, antimutagenic, and antimicrobial activities of crude extracts (CE) and peptide fractions (<3 and 3-10 kDa) obtained from fermented milks with specific Lactobacillus plantarum strains...
November 16, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27863712/glycoform-analysis-of-alpha1-acid-glycoprotein-by-capillary-electrophoresis
#11
Chenhua Zhang, David S Hage
A relatively fast and reproducible CE separation was developed for the glycoform analysis of α1-acid glycoprotein (AGP). Factors that were considered included the pH for this separation and various techniques for coating the capillary and/or to minimize electroosmotic flow and protein adsorption. Optimum resolution of the AGP glycoforms was obtained at pH 4.2 with a running buffer containing 0.1% Brij 35 and by using static and dynamic coatings of PEO on the capillary. These conditions made it possible to separate nine AGP glycoform bands in about 20min...
December 2, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27862039/acupuncture-injection-for-field-amplified-sample-stacking-and-glass-microchip-based-capillary-gel-electrophoresis
#12
Ji Won Ha, Jong Hoon Hahn
Acupuncture sample injection is a simple method to deliver well-defined nL-scale sample plugs in polydimethylsiloxane (PDMS) microfluidic channels. This acupuncture injection method in microchip CE has several advantages, including minimization of sample consumption, the capability of serial injections of different sample solutions into the same microchannel, and the capability of injecting sample plugs into any desired position of a microchannel. Herein, we demonstrate that the simple and cost-effective acupuncture sample injection method can be used for PDMS microchip-based field amplified sample stacking (FASS) in the most simplified straight channel by applying a single potential...
November 10, 2016: Electrophoresis
https://www.readbyqxmd.com/read/27852380/-clinical-analysis-of-endovascular-strategies-in-the-treatment-of-vertebrobasilar-dissecting-aneurysms
#13
Y Z Zang, Z G Wang, C W Wang, Y Zhang, X Ding, X F Wang
Objective: To evaluate the efficacy and safety of endovascular strategies for vertebrobasilar dissecting aneurysm (VBDA) and analyze advantages and disadvantages of different endovascular methods for the treatment of VBDA through long-term follow-up. Methods: Retrospectively analyzing was used for the clinical information of 45 cases of vertebrobasilar dissecting aneurysms treated with endovascular methods in our department from January 2007 to December 2015. The treatment modalities were classified into reconstructive surgery and destructive surgery that were based on parent artery preservation...
November 8, 2016: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/27836654/follow-up-of-intracranial-aneurysms-treated-with-stent-assisted-coiling-comparison-of-contrast-enhanced-mra-time-of-flight-mra-and-digital-subtraction-angiography
#14
David Marciano, Sébastien Soize, Georgios Metaxas, Christophe Portefaix, Laurent Pierot
BACKGROUND AND PURPOSE: Data about non-invasive follow-up of aneurysm after stent-assisted coiling is scarce. We aimed to compare time-of-flight (TOF) magnetic resonance angiography (MRA) (3D-TOF-MRA) and contrast-enhanced MRA (CE-MRA) at 3-Tesla, with digital subtraction angiography (DSA) for evaluating aneurysm occlusion and parent artery patency after stent-assisted coiling. MATERIALS AND METHODS: In this retrospective single-center study, patients were included if they had an intracranial aneurysm treated by stent-assisted coiling between March 2008 and June 2015, followed with both MRA sequences (3D-TOF-MRA and CE-MRA) at 3-Tesla and DSA, performed in an interval<48hours...
November 8, 2016: Journal of Neuroradiology. Journal de Neuroradiologie
https://www.readbyqxmd.com/read/27836651/magnetic-resonance-imaging-characteristics-of-ischemic-stroke-in-an-afro-caribbean-population-a-1-year-prospective-mri-study-on-534%C3%A2-consecutive-patients
#15
M Mejdoubi, A Signate, S Colombani, A Arrigo, S Olindo
PURPOSES: Few population-based MRI studies on stroke, particularly in African-descent populations, are available. Based on a 1-year Afro-Caribbean population-based study MRI, ischemic stroke characteristics were extensively analyzed. METHODS: All strokes occurring in Martinique (390,371 inhabitants) were prospectively included. Ascertainment was based, whenever possible, on MRI. All patients were categorized as single- (subclassified as cortical, cortical-subcortical, subcortical, lacunar) or multiple-lesion pattern, and vascular (single, multiple or junctional) territory...
November 8, 2016: Journal of Neuroradiology. Journal de Neuroradiologie
https://www.readbyqxmd.com/read/27830849/review-imaging-technologies-for-flow-cytometry
#16
REVIEW
Yuanyuan Han, Yi Gu, Alex Ce Zhang, Yu-Hwa Lo
High-throughput single cell imaging is a critical enabling and driving technology in molecular and cellular biology, biotechnology, medicine and related areas. Imaging flow cytometry combines the single-cell imaging capabilities of microscopy with the high-throughput capabilities of conventional flow cytometry. Recent advances in imaging flow cytometry are remarkably revolutionizing single-cell analysis. This article describes recent imaging flow cytometry technologies and their challenges.
November 10, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27826994/diagnostic-efficacy-of-single-pass-abdominal-multidetector-row-ct-prospective-evaluation-of-a-low-dose-protocol
#17
Luigi Camera, Immacolata Liccardo, Federica Romano, Raffaele Liuzzi, Antonio Rispo, Massimo Imbriaco, Anna Testa, Gaetano Luglio, Simona De Fronzo, Fabiana Castiglione, Luigi Bucci, Arturo Brunetti
OBJECTIVES: To evaluate the diagnostic efficacy of single-pass contrast-enhanced multi-detector CT (CE-MDCT) performed with a low radiation-high contrast (LR-HC) dose protocol in selected patients with non traumatic acute bowel disease. METHODS: Sixty-five (32 M; 33 F; aged 20-67 years) consecutive patients with non traumatic acute bowel disease underwent single-pass CE-MDCT performed 70-100 sec after i.v. bolus injection of a non ionic iodinated contrast media (370 mgI/ml)...
November 9, 2016: British Journal of Radiology
https://www.readbyqxmd.com/read/27816804/persistent-changes-in-lipoprotein-lipids-after-a-single-infusion-of-ascending-doses-of-mdco-216-apoa-imilano-popc-in-healthy-volunteers-and-stable-coronary-artery-disease-patients
#18
Herman J Kempen, Monica Gomaraschi, Sara Simonelli, Laura Calabresi, Matthijs Moerland, James Otvos, Elias Jeyarajah, David Kallend, Peter L J Wijngaard
BACKGROUND AND AIMS: Effects of single ascending doses of MDCO-216 on plasma lipid and lipoprotein levels were assessed in human healthy volunteers and in patients with stable coronary artery disease (CAD). METHODS: MDCO-216 was infused at a single dose of 5, 10, 20, 30 or 40 mg/kg over 2 h and blood was collected at 2, 4, 8, 24, 48, 168 and 720 h after start of infusion (ASOI). Lipoprotein lipids were assessed by FLPC and by 1H NMR. RESULTS: Plasma concentrations of free cholesterol (FC) displayed a rapid and dose-dependent rise, peaking at 8 h, but remaining above baseline until 48 h ASOI, whereas levels of esterified cholesterol (CE) increased at lower doses but not at higher doses, and even decreased below baseline at the highest dose...
October 24, 2016: Atherosclerosis
https://www.readbyqxmd.com/read/27813287/efficient-decarbonylation-of-furfural-to-furan-catalyzed-by-zirconia-supported-palladium-clusters-with-low-atomicity
#19
Tamao Ishida, Kurumi Kume, Kota Kinjo, Tetsuo Honma, Kengo Nakada, Hironori Ohashi, Takushi Yokoyama, Akiyuki Hamasaki, Haruno Murayama, Yusuke Izawa, Masaru Utsunomiya, Makoto Tokunaga
Decarbonylation of furfural to furan was efficiently catalyzed by ZrO2 -supported Pd clusters in the liquid phase under a N2 atmosphere without additives. Although Pd/C and Pd/Al2 O3 have frequently been used for decarbonylation, Pd/ZrO2 exhibited superior catalytic performance compared with these conventional catalysts. Transmission electron microscopy and X-ray absorption fine structure measurements revealed that the size of the Pd particles decreased with an increase in the specific surface area of ZrO2 ...
November 4, 2016: ChemSusChem
https://www.readbyqxmd.com/read/27811339/leptomeningeal-contrast-enhancement-is-associated-with-progression-of-cortical-atrophy-in-ms-a-retrospective-pilot-observational-longitudinal-study
#20
Robert Zivadinov, Deepa P Ramasamy, Manuela Vaneckova, Sirin Gandhi, Avinash Chandra, Jesper Hagemeier, Niels Bergsland, Paul Polak, Ralph Hb Benedict, David Hojnacki, Bianca Weinstock-Guttman
BACKGROUND: Leptomeningeal contrast enhancement (LM CE) has been recently described in multiple sclerosis (MS) patients as a potential in vivo marker of cortical pathology. OBJECTIVES: To investigate the association of LM CE and development of cortical atrophy in 50 MS patients (27 relapsing-remitting (RR) and 23 secondary-progressive (SP)) followed for 5 years. METHODS: The presence and number of LM CE foci were assessed only at the 5-year follow-up using three-dimensional (3D) fluid-attenuated inversion recovery magnetic resonance imaging (MRI) sequence obtained 10 minutes after single dose of gadolinium injection on 3T scanner...
November 3, 2016: Multiple Sclerosis: Clinical and Laboratory Research
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