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https://www.readbyqxmd.com/read/28425271/molecular-characteristics-and-antibiotic-resistance-of-acinetobacter-baumanniibeta-lactamase-producing-isolates-a-predominance-of-intrinsic-blaoxa-51-and-detection-of-tem-and-ctx-m-genes
#1
Amir Ibrahimagić, Farah Kamberović, Selma Uzunović, Branka Bedenić, Emina Idrizović
BACKGROUND/AIM: The aim of this study was to determine the molecular characteristics and antibiotic resistance of 13 (10 inpatient and three outpatient) Acinetobacter baumannii beta-lactamase-producing isolates collected in Bosnia and Herzegovina between December 2009 and May 2010. MATERIALS AND METHODS: Susceptibility testing was performed by disk diffusion and broth microdilution methods. The modified Hodge and combined disk test with EDTA/phenylboronic acid was used to screen for carbapenemase production...
April 18, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28404680/development-and-validation-of-a-lateral-flow-immunoassay-for-the-rapid-detection-of-ndm-producing-enterobacteriaceae
#2
Hervé Boutal, Thierry Naas, Karine Devilliers, Saoussen Oueslati, Laurent Dortet, Sandrine Bernabeu, Stéphanie Simon, Hervé Volland
The global spread of carbapenemase-producing Enterobacteriaceae (CPE) that are often resistant to most, if not all, classes of antibiotics is a major public health concern. NDM-1 carbapenemase is among the most worrisome carbapenemases given its rapid worldwide spread. We have developed and evaluated a lateral flow immunoassay (LFIA, called NDM LFIA) for rapid and reliable detection of NDM-like carbapenemase-producing Enterobacteriaceae from culture colonies.We evaluated the NDM LFIA using 175 reference enterobacterial isolates with characterized ß-lactamase gene content and 74 non-duplicate consecutive carbapenem-resistant clinical isolates referred for expertise to the French National Reference Center (NRC) for antibiotic resistance during a one-week period (in June 2016)...
April 12, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28357923/performance-of-carbanp-and-cim-tests-in-oxa-48-carbapenemase-producing-enterobacteriaceae
#3
Serap Süzük Yıldız, Banu Kaşkatepe, Havva Avcıküçük, Şükran Öztürk
This study applied two phenotypic tests, namely "Carbapenemase Nordmann-Poirel" (CarbaNP) test and "Carbapenem Inactivation Method" (CIM), against the isolates carrying the carbapenem resistance genes. The study included 83 carbapenem-resistant Enterobacteriaceae isolates producing oxacillinase-48 (OXA-48) and 30 carbapenem-sensitive Enterobacteriaceae isolates. Out of the total isolates studied, 77 isolates (92.77%) were identified as Klebsiella pneumoniae and six isolates (7.23%) were identified as Escherichia coli by Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry...
March 1, 2017: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/28268175/dissemination-of-carbapenem-resistant-acinetobacter-baumannii-in-patients-with-burn-injuries
#4
Saeed Shoja, Mojtaba Moosavian, Soodabeh Rostami, Abbas Farahani, Amir Peymani, Khadijeh Ahmadi, Nasim Ebrahimifard
BACKGROUND: Carbapenem-resistant Acinetobacter baumannii has emerged as an important cause of infection in burn patients. This study aimed to characterize the antimicrobial susceptibility pattern, determine the prevalence of oxacillinase and metallo-beta-lactamase (MBL) genes, and type the A. baumannii isolates obtained from burn patients. METHODS: During a 1-year period, a total of 40 nonduplicated isolates of A. baumannii were obtained from burn patients who were hospitalized in the Taleghani Burn Hospital in Ahvaz, in the southwest of Iran...
March 3, 2017: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/28251105/carbapenemase-producing-enterobacteriaceae-risk-factors-for-infection-and-impact-of-resistance-on-outcomes
#5
Shanthi Mariappan, Uma Sekar, Arunagiri Kamalanathan
BACKGROUND: Carbapenemase-producing Enterobacteriaceae (CPE) have increased in recent years leading to limitations of treatment options. The present study was undertaken to detect CPE, risk factors for acquiring them and their impact on clinical outcomes. METHODS: This retrospective observational study included 111 clinically significant Enterobacteriaceae resistant to cephalosporins subclass III and exhibiting a positive modified Hodge test. Screening for carbapenemase production was done by phenotypic methods, and polymerase chain reaction was performed to detect genes encoding them...
January 2017: International Journal of Applied and Basic Medical Research
https://www.readbyqxmd.com/read/28191309/co-existence-of-blaoxa-23-and-blandm-1-genes-of-acinetobacter-baumannii-isolated-from-nepal-antimicrobial-resistance-and-clinical-significance
#6
Prabhu Raj Joshi, Mahesh Acharya, Trishna Kakshapati, Udomluk Leungtongkam, Rapee Thummeepak, Sutthirat Sitthisak
BACKGROUND: Molecular analysis of carbapenem-resistant genes in Acinetobacter baumannii, an emerging pathogen, is less commonly reported from Nepal. In this study we determined the antibiotic susceptibility profile and genetic mechanism of carbapenem resistance in clinical isolates of A. baumannii. METHODS: A. baumannii were isolated from various clinical specimens and identified based on Gram staining, biochemical tests, and PCR amplification of organism specific 16S rRNA and blaOXA-51 genes...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/27988171/evaluation-of-a-rapid-immunochromatographic-test-for-the-detection-of-oxa-48-carbapenemase
#7
Elisa Rubio, Yuliya Zboromyrska, Cristina Pitart, Irene Campo, Izaskun Alejo-Cancho, Assumpta Fasanella, Andrea Vergara, Francesc Marco, Jordi Vila
We evaluated the OXA-48K-Set, a rapid immunochromatographic test for the detection of Oxacillinase-48 (OXA-48) carbapenemases, among 37 strains expressing OXA-48 and OXA-48-like carbapenemases and 20 additional strains harboring other β-lactamases. The test showed 100% sensitivity and specificity and the results were obtained in 15minutes.
March 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27934820/molecular-characterisation-of-antimicrobial-resistance-in-pseudomonas-aeruginosa-and-acinetobacter-baumannii-during-2014-and-2015-collected-across-india
#8
MULTICENTER STUDY
A K Pragasam, S Vijayakumar, Y D Bakthavatchalam, A Kapil, B K Das, P Ray, V Gautam, S Sistla, S C Parija, K Walia, V C Ohri, S Anandan, B Veeraraghavan
BACKGROUND: Surveillance of antimicrobial resistance (AMR) is of great importance. Pseudomonas aeruginosa and Acinetobacter baumannii are important pathogens and emergence of resistance in these have increased the morbidity and mortality rates. This surveillance study was initiated by the Government of India - Indian Council of Medical Research. The aim of this study is to determine the antimicrobial susceptibility profile and to characterise the enzyme mediated antimicrobial resistance such as extended spectrum beta-lactamases (ESBLs) and carbapenemases among multidrug-resistant (MDR) P...
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27933276/acinetobacter-baumannii-isolated-from-lebanese-patients-phenotypes-and-genotypes-of-resistance-clonality-and-determinants-of-pathogenicity
#9
Elias Dahdouh, Micheline Hajjar, Monica Suarez, Ziad Daoud
Introduction:Acinetobacter baumannii is a nosocomial pathogen that usually affects critically ill patients. High mortality rates have been associated with MDR A. baumannii infections. Carbapenem resistance among these isolates is increasing worldwide and is associated with certain International Clones (ICs) and oxacillinases (OXAs). Moreover, this organism possesses a wide range of virulence factors, whose expression is not yet fully understood. In this study, clinical A. baumannii isolates are characterized in terms of antibiotic resistance, mechanisms of carbapenem resistance, clonality, and virulence...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27706778/prevalence-of-%C3%AE-lactamase-classes-a-c-and-d-among-clinical-isolates-of-pseudomonas-aeruginosa-from-a-tertiary-level-hospital-in-bangkok-thailand
#10
C Katvoravutthichai, K Boonbumrung, R Tiyawisutsri
Pseudomonas aeruginosa is one of the most important causes of nosocomial infection and it has increasing resistance to many antimicrobial agents. β-lactamase production is the most frequent mechanism for β-lactam resistance in P. aeruginosa. We evaluated the prevalence of β-lactamase genes in P. aeruginosa for classes A, C, and D by polymerase chain reaction, and investigated clonal diversity by pulsed-field gel electrophoresis (PFGE). We used the disk diffusion method to test 118 non-duplicate clinical isolates of P...
September 23, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27705609/emergence-of-oxacillinases-in-environmental-carbapenem-resistant-acinetobacter-baumannii-associated-with-clinical-isolates
#11
Ivana Goic-Barisic, Jasna Hrenovic, Ana Kovacic, Martina Šeruga Musić
Six carbapenem-resistant isolates of Acinetobacter baumannii were recovered from untreated and treated municipal wastewater of the capital city of Zagreb, Croatia. Molecular identification of environmental isolates of A. baumannii was performed by amplification, sequencing, and phylogenetic analyses of rpoB gene. The presence of blaOXA genes encoding OXA-type carbapenemases (OXA-51-like, OXA-23, and OXA-40-like) was confirmed by multiplex PCR and sequencing. Phylogenetic analyses corroborated the affiliation of detected blaOXA genes to three different clusters and showed association of environmental OXAs with those described from clinical isolates...
October 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27598629/the-role-of-the-genetic-elements-bla-oxa-and-is-aba-1-in-the-acinetobacter-calcoaceticus-acinetobacter-baumannii-complex-in-carbapenem-resistance-in-the-hospital-setting
#12
Vanessa Cristine Kobs, Jéssica Augustini Ferreira, Thaís Alexandra Bobrowicz, Leslie Ecker Ferreira, Roseneide Campos Deglmann, Glauco Adrieno Westphal, Paulo Henrique Condeixa de França
INTRODUCTION: Members of the Acinetobacter genus are key pathogens that cause healthcare-associated infections, and they tend to spread and develop new antibiotic resistance mechanisms. Oxacillinases are primarily responsible for resistance to carbapenem antibiotics. Higher rates of carbapenem hydrolysis might be ascribed to insertion sequences, such as the ISAba1 sequence, near bla OXA genes. The present study examined the occurrence of the genetic elements bla OXA and ISAba1 and their relationship with susceptibility to carbapenems in clinical isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex...
July 2016: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/27597874/diversity-of-molecular-mechanisms-conferring-carbapenem-resistance-to-pseudomonas-aeruginosa-isolates-from-saudi-arabia
#13
Mohamed H Al-Agamy, Katy Jeannot, Taghrid S El-Mahdy, Hassan A Samaha, Atef M Shibl, Patrick Plésiat, Patrice Courvalin
Background. This study described various molecular and epidemiological characters determining antibiotic resistance patterns in Pseudomonas aeruginosa isolates. Methods. A total of 34 carbapenem-resistant P. aeruginosa clinical isolates were isolated from samples collected at a tertiary hospital in Riyadh, Saudi Arabia, from January to December 2011. Susceptibility testing, serotyping, molecular characterization of carbapenem resistance, and pulsed-field gel electrophoresis (PFGE) were performed. Results. All isolates were resistant to ceftazidime, and more than half were highly resistant (minimum inhibitory concentration (MIC) > 256 mg/L)...
2016: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/27584588/reducing-the-impact-of-carbapenem-resistant-enterobacteriaceae-on-vulnerable-patient-groups-what-can-be-done
#14
Gökhan Metan, Murat Akova
PURPOSE OF REVIEW: Carbapenem-resistant Enterobacteriaceae (CRE) is a worldwide challenge and associated with a high mortality rate in critically ill patients. This review focused on rapid diagnosis, optimization of antimicrobial therapy, and implication of effective infection control precautions to reduce impact of CRE on vulnerable patients. RECENT FINDINGS: Several new diagnostic assays have recently been described for the early diagnosis of CRE. Retrospective studies are supportive for colistin plus meropenem combination for the treatment of CRE infections; however, solid evidence is still lacking...
December 2016: Current Opinion in Infectious Diseases
https://www.readbyqxmd.com/read/27578505/prevalence-and-molecular-characteristics-of-carbapenemase-producing-enterobacteriaceae-from-five-hospitals-in-korea
#15
Seok Hoon Jeong, Han Sung Kim, Jae Seok Kim, Dong Hoon Shin, Hyun Soo Kim, Min Jeong Park, Saeam Shin, Jun Sung Hong, Seung Soon Lee, Wonkeun Song
BACKGROUND: The emergence of carbapenemase-producing Enterobacteriaceae (CPE) represents a major clinical problem because these bacteria are resistant to most antibiotics. CPE remain relatively uncommon in Korea. We report the prevalence, clinical characteristics, and molecular epidemiology of CPE isolates collected from five university hospitals in Korea. METHODS: Between January and December 2015, 393 non-duplicated isolates that were nonsusceptible to ertapenem were analyzed...
November 2016: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/27553951/molecular-characterization-of-invasive-carbapenem-resistant-acinetobacter-baumannii-from-a-tertiary-care-hospital-in-south-india
#16
Saranya Vijayakumar, Radha Gopi, Priya Gunasekaran, Manjurekar Bharathy, Kamini Walia, Shalini Anandan, Balaji Veeraraghavan
INTRODUCTION: Acinetobacter baumannii is an important opportunistic pathogen responsible for causing nosocomial infections. Carbapenems are considered to be the drug of choice to treat infections caused by multidrug-resistant A. baumannii. The prevalent mechanism of carbapenem resistance in A. baumannii is enzymatic degradation by β-lactamases. Therefore, the aim of the study is to determine the prevalence and distribution of molecular determinants among the clinical isolates of carbapenem-resistant A...
September 2016: Infectious Diseases and Therapy
https://www.readbyqxmd.com/read/27551277/carbapenem-resistance-in-acinetobacter-baumannii-and-other-acinetobacter-spp-causing-neonatal-sepsis-focus-on-ndm-1-and-its-linkage-to-isaba125
#17
Somdatta Chatterjee, Saswati Datta, Subhasree Roy, Lavanya Ramanan, Anindya Saha, Rajlakshmi Viswanathan, Tapas Som, Sulagna Basu
Carbapenem-resistant determinants and their surrounding genetic structure were studied in Acinetobacter spp. from neonatal sepsis cases collected over 7 years at a tertiary care hospital. Acinetobacter spp. (n = 68) were identified by ARDRA followed by susceptibility tests. Oxacillinases, metallo-β-lactamases (MBLs), extended-spectrum β-lactamases and AmpCs, were detected phenotypically and/or by PCR followed by DNA sequencing. Transconjugants possessing the bla NDM-1(New Delhi metallo-β-lactamase) underwent further analysis for plasmids, integrons and associated genes...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27540456/characterization-of-oxacillinase-and-metallo-%C3%AE-lactamas-genes-and-molecular-typing-of-clinical-isolates-of-acinetobacter-baumannii-in-ahvaz-south-west-of-iran
#18
Saeed Shoja, Mojtaba Moosavian, Soodabeh Rostami, Fariba Abbasi, Mohammad Amin Tabatabaiefar, Amir Peymani
BACKGROUND: Carbapenem resistant Acinetobacter baumannii is an important nosocomial pathogen associated with a variety of infections. OBJECTIVES: The current study aimed to characterize the antimicrobial susceptibility, analyze the prevalence of oxacillinase and metallo-β-lactamase (MBL) genes and molecular typing of clinical isolates of A. baumannii. MATERIALS AND METHODS: A total of 124 non-repetitive isolates of A. baumannii were collected from various clinical specimens in two teaching hospitals in Ahvaz, south-west of Iran...
May 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27525401/-evaluation-of-the-efficacy-of-colistin-sulbactam-combination-on-carbapenem-resistant-acinetobacter-baumannii-strains
#19
Yeliz Çetinkol, Murat Telli, Arzu Altunçekiç Yıldırım, Mustafa Kerem Çalgın
Acinetobacter baumannii strains, are opportunistic pathogens that cause severe nosocomial infections that are difficult to treat due to development of resistance to multiple antibiotics. As the antibiotic choices to be used in treatment are limited, combinations of a variety of antibiotics are used. The aims of this study were to identify the minimal inhibitory concentration (MIC) values of colistin and sulbactam against A.baumannii isolates and to determine the in vitro activity of colistin-sulbactam combination...
July 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/27499434/characterisation-of-class-3-integrons-with-oxacillinase-gene-cassettes-in-hospital-sewage-and-sludge-samples-from-france-and-luxembourg
#20
Pierrette Landrie Simo Tchuinte, Thibault Stalder, Silvia Venditti, Antoinette Ngandjio, Christophe Dagot, Marie-Cécile Ploy, Olivier Barraud
In this study, antibiotic resistance class 3 integrons in Gram-negative bacteria isolated from hospital sewage and sludge and their genetic contents were characterised. Two samples of hospital effluent from France and Luxembourg and one sample of sludge from a wastewater treatment plant in France were collected in 2010 and 2011. Bacteria were cultured on selective agar plates and integrons were detected in colonies by quantitative PCR. Integron gene cassette arrays and their genetic environments were analysed by next-generation sequencing...
October 2016: International Journal of Antimicrobial Agents
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