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https://www.readbyqxmd.com/read/29434938/genetic-characterization-and-in-vitro-activity-of-antimicrobial-combinations-of-multidrug-resistant-acinetobacter-baumannii-from-a-general-hospital-in-china
#1
Fang Chen, Ling Wang, Min Wang, Yixin Xie, Xiaomeng Xia, Xianping Li, Yanhua Liu, Wei Cao, Tingting Zhang, Pengling Li, Min Yang
The present study aimed to develop a rational therapy based on the genetic epidemiology, molecular mechanism evaluation and in vitro antibiotic combinations activity in multidrug-resistant Acinetobacter baumannii (MDRAB). MDRAB was screened by the Kirby-Bauer method. The random amplified polymorphic DNA technique was used to establish genetic fingerprinting, and a series of resistance genes were detected by polymerase chain reaction. Antimicrobial agents including amikacin (AK), cefoperazone/sulbactam (SCF I/II), meropenem (MEM), minocycline (MINO) and ciprofloxacin (CIP) were used to determine the minimum inhibitory concentrations (MICs) and interactions between antibiotics by the broth microdilution method and chequerboard assays...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29365094/a-multiplex-lateral-flow-immunoassay-for-the-rapid-identification-of-ndm-kpc-imp-and-vim-type-and-oxa-48-like-carbapenemase-producing-enterobacteriaceae
#2
Hervé Boutal, Anaïs Vogel, Sandrine Bernabeu, Karine Devilliers, Elodie Creton, Garence Cotellon, Marc Plaisance, Saoussen Oueslati, Laurent Dortet, Agnès Jousset, Stéphanie Simon, Thierry Naas, Hervé Volland
Objectives: The global spread of carbapenemase-producing Enterobacteriaceae represents a substantial challenge in clinical practice and rapid and reliable detection of these organisms is essential. The aim of this study was to develop and validate a lateral flow immunoassay (Carba5) for the detection of the five main carbapenemases (KPC-, NDM-, VIM- and IMP-type and OXA-48-like). Methods: Carba5 was retrospectively and prospectively evaluated using 296 enterobacterial isolates from agar culture...
January 22, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29348071/a-focused-fragment-library-targeting-the-antibiotic-resistance-enzyme-oxacillinase-48-synthesis-structural-evaluation-and-inhibitor-design
#3
Sundus Akhter, Bjarte Aarmo Lund, Aya Ismael, Manuel Langer, Johan Isaksson, Tony Christopeit, Hanna-Kirsti S Leiros, Annette Bayer
β-Lactam antibiotics are of utmost importance when treating bacterial infections in the medical community. However, currently their utility is threatened by the emergence and spread of β-lactam resistance. The most prevalent resistance mechanism to β-lactam antibiotics is expression of β-lactamase enzymes. One way to overcome resistance caused by β-lactamases, is the development of β-lactamase inhibitors and today several β-lactamase inhibitors e.g. avibactam, are approved in the clinic. Our focus is the oxacillinase-48 (OXA-48), an enzyme reported to spread rapidly across the world and commonly identified in Escherichia coli and Klebsiella pneumoniae...
December 30, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29332105/the-emergence-of-carbapenem-resistant-klebsiella-pneumoniae-isolates-producing-oxa-48-and-ndm-in-the-southern-asir-province-saudi-arabia
#4
Ibrahim A Al-Zahrani, Bander A Alsiri
To identify the prevalence of carbapenem-resistant Klebsiella pneumoniae (CRKP) and the most common types of cabapenemases among CRKP in the Southern (Asir) province hospitals, Saudi Arabia. Methods: The cross-sectional study was conducted between late April and September in 2015. A total of 54 Klebsiella pneumoniae (K. pneumoniae) isolates with reduced sensitivity to carbapenems were obtained from various clinical specimens of the 2 largest hospitals in the Southern province. Minimum inhibitory concentrations (MICs) of carbapenems were confirmed using E-test...
January 2018: Saudi Medical Journal
https://www.readbyqxmd.com/read/29305547/evaluation-of-the-amplidiag-carbar-vre-kit-for-the-accurate-detection-of-carbapenemase-producing-bacteria
#5
Saoussen Oueslati, Delphine Girlich, Laurent Dortet, Thierry Naas
As carbapenemase-producing Gram-negative bacilli (CP-GNB) ( Enterobacteriaceae , Pseudomonadaceae and Acinetobacter spp.) are becoming a major public health issue, there is an urgent need for accurate and fast diagnostic tests. Amplidiag® Carba-R+VRE assay is a multiplex nucleic acid-based in-vitro diagnostic test intended for the detection of CP-GNB and vancomycin resistant enterococci (VRE) from cultured colonies.We have evaluated its ability to detect carbapenemases genes in 100 well-characterized GNB, and in 200 consecutive enterobacterial isolates with reduced susceptibility to carbapenems and that were referred to the French National Reference Center for carbapenem-resistance...
January 5, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29283077/species-distribution-sequence-types-and-antimicrobial-resistance-of-acinetobacter-spp-from-cystic-fibrosis-patients
#6
G A Rocha, D F Lima, E R Rodrigues, R S Leão, T W Folescu, M C Firmida, R W F Cohen, R M Albano, E A Marques
Acinetobacter spp. are important healthcare pathogens, being closely linked to antibiotic resistance and outbreaks worldwide. Although such species are rarely observed in patients with cystic fibrosis (CF), we describe the characteristics of 53 strains of Acinetobacter spp. isolated from the sputum of 39 Brazilian patients with CF. The species distribution was A. baumannii (n = 29), A. pittii (n = 13), A. nosocomialis (n = 8), A. seifertii (n = 1), A. soli (n = 1) and A. variabilis (n = 1) determined by partial rpoB gene sequencing...
December 28, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/29277554/arrival-of-carbapenem-hydrolyzing-oxacillinases-in-acinetobacter-baumannii-in-bosnia-and-herzegovina
#7
Tanja Petrović, Selma Uzunović, Ivan Barišić, Josefa Luxner, Andrea Grisold, Gernot Zarfel, Amir Ibrahimagić, Sanja Jakovac, Domagoj Slaćanac, Branka Bedenić
No abstract text is available yet for this article.
December 22, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29152950/investigation-of-class-d-beta-lactamases-causing-carbapenem-resistance-in-clinical-acinetobacter-baumannii-isolates
#8
Iskandar Davandeh, Bayrı Eraç, Sabire Şöhret Aydemir
Background/aim: Acinetobacter baumannii is an important causative agent of nosocomial infections, and carbapenems have been frequently used in the treatment of these infections. This study was designed to investigate the prevalence of primary carbapenem hydrolyzing oxacillinase (CHO) types in clinical A. bumannii strains. Materials and methods: Minimum inhibitory concentration (MIC) values of 76 imipenem nonsusceptible A. baumannii strains, isolated from a tertiary care hospital, were determined by microdilution method...
November 13, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/29127051/genomic-analysis-of-in-vivo-acquired-resistance-to-colistin-and-rifampin-in-acinetobacter-baumannii
#9
Nadia Jaidane, Thierry Naas, Wejdene Mansour, Bechir Ben Radhia, Sofiene Jerbi, Noureddine Boujaafar, Olfa Bouallegue, Rémy A Bonnin
Acinetobacter baumannii is an opportunistic pathogen in health-care facilities responsible for nosocomial infections mostly in immune-compromised patients. Colistin resistance is increasingly reported worldwide in that species. We describe here in vivo selection of colistin and rifampin resistance in a carbapenem-resistant A. baumannii (Ab). Antimicrobial susceptibility testing, plasmid analysis, and Whole Genome Sequencing (WGS) were performed to fully characterize the resistome of AbS1 and AbS2 clinical isolates...
November 7, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29075243/acinetobacter-baumannii-virulence-traits-a-comparative-study-of-a-novel-sequence-type-with-other-italian-endemic-international-clones
#10
Cecilia Ambrosi, Daniela Scribano, Marta Aleandri, Carlo Zagaglia, Laura Di Francesco, Lorenza Putignani, Anna Teresa Palamara
Carbapenem-resistant Acinetobacter baumannii (CRAb) have emerged in recent decades as major causes of nosocomial infections. Resistance is mainly due to overexpression of intrinsic and/or acquired carbapenemases, especially oxacillinases (OXA). In Italy, although the sequence type (ST) 2 and the ST78 are the most frequently detected, we recently reported ST632, a single locus variant of ST2. Therefore, this study was aimed at unraveling common bacterial surface virulence factors involved in pathogenesis and antibiotic resistance in representative CRAb of these ST genotypes...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29063891/identification-of-carbapenemase-mediated-resistance-among-enterobacteriaceae-bloodstream-isolates-a-molecular-study-from-india
#11
Srujana Mohanty, Mittal Gajanand, Rajni Gaind
Acquired resistance in carbapenem-resistant Enterobacteriaceae (CRE) conferred by carbapenemases is a major concern worldwide. Consecutive, non-duplicate isolates of Escherichia coli (EC) and Klebsiella pneumoniae from clinically diagnosed bloodstream infections were screened for the presence of carbapenem resistance by standard disk-diffusion method and minimum inhibitory concentration breakpoints using the Clinical and Laboratory Standards Institute guidelines. Carbapenemase-encoding genes were amplified by polymerase chain reaction...
July 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28980827/emergence-of-isaba1-harboring-carbapenem-resistant%C3%A2-acinetobacter-baumannii-isolates-in-pakistan
#12
Mohsin Khurshid, Muhammad Hidayat Rasool, Usman Ali Ashfaq, Bilal Aslam, Muhammad Waseem
AIM: The ISAba1 insertion sequence supports the overexpression of various oxacillinases genes by providing promoter and therefore, confers carbapenem resistance. The study aimed to determine the clonal diversity, genetic attributes of carbapenem resistance and the role of ISAba1 in the expression of oxacillinases genes among Acinetobacter baumannii clinical isolates. MATERIALS & METHODS: PCR was performed to amplify various antimicrobial resistance determinants and ISAba1 insertion elements in 137 A...
October 5, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28954001/glimpse-into-the-genome-sequence-of-a-multidrug-resistant-acinetobacter-pittii-st950-clinical-isolate-carrying-the-blaoxa-72-and-blaoxa-533-genes-in-china
#13
Zhi Ruan, Yan Chen, Jianfeng Wang
The development of carbapenem-resistant Acinetobacter species is of serious concern in the hospital settings and naturally occurring oxacillinase genes (blaOXA) have been identified in several Acinetobacter species. In this study, we report the genome sequence of A. pittii TCM178 belongs to ST950, a multidrug-resistant isolate that harbored the blaOXA-72 and blaOXA-533 genes simultaneous. The genome size was estimated to be 3,789,564 bp with 3,501 predicted coding regions, and G+C content is 37.60%. Our findings have raised awareness of the possible constitution of a reservoir for peculiar carbapenemase genes in A...
October 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28735048/emergence-of-different-acinetobacter-baumannii-clones-in-a-croatian-hospital-and-correlation-with-antibiotic-susceptibility
#14
Ranko Ladavac, Branka Bedenić, Mirna Vranić-Ladavac, Nada Barišić, Natalie Karčić, Karoline Pompe, Antun Ferenčić, Aleksandar Stojanović, Harald Seifert, Stjepan Katić, Paul G Higgins
OBJECTIVES: During routine diagnostic laboratory work, the clinical microbiologist observed an increase of Acinetobacter baumannii isolates with three different carbapenem susceptibility patterns: susceptible, intermediate and resistant. Isolates belonging to the same carbapenem susceptibility phenotype exhibited identical susceptibility/resistance patterns to non-β-lactam antibiotics. This prompted us to analyse the mechanisms of carbapenem-resistance and the molecular epidemiology of the isolates...
September 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28692823/high-hiv-infection-prevalence-in-a-group-of-men-who-have-sex-with-men
#15
Raquel Maria Cardoso Torres, Marly Marques da Cruz, André Reynaldo Santos Périssé, Denise Ribeiro Franqueira Pires
Brazil is characterized by a concentrated AIDS epidemic, it has a prevalence of less than 1% in the general population. However, there are higher rates in specific populations, especially in men who have sex with men. The study's aim was to analyze the association between sociodemographic characteristics, sexual practices, sexual behaviors and the HIV infection in a group of men who have sex with men. Secondary data was collected between June 2014 and September 2015 in a research of cross-sectional design in the city of Rio de Janeiro, Brazil...
July 8, 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28652234/meropenem-vaborbactam-tested-against-contemporary-gram-negative-isolates-collected-worldwide-during-2014-including-carbapenem-resistant-kpc-producing-multidrug-resistant-and-extensively-drug-resistant-enterobacteriaceae
#16
Mariana Castanheira, Michael D Huband, Rodrigo E Mendes, Robert K Flamm
We evaluated the activity of meropenem-vaborbactam against contemporary nonfastidious Gram-negative clinical isolates, including Enterobacteriaceae isolates with resistance phenotypes and carbapenemase genotypes. Meropenem-vaborbactam (inhibitor at 8 μg/ml) and comparators were susceptibility tested by reference broth microdilution methods against 14,304 Gram-negative clinical isolates collected worldwide during 2014. Carbapenemase-encoding genes were screened by PCR and sequencing. Meropenem-vaborbactam (MIC50/90, ≤0...
September 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28487993/acinetobacter%C3%A2-baumannii-quorum-sensing-signalling-molecule-induces-the-expression-of-drug-resistance-genes
#17
Yi Dou, Fei Song, Feng Guo, Zengding Zhou, Cailian Zhu, Jun Xiang, Jingning Huan
Quorum-sensing signalling molecules such as N‑acyl homoserine lactones (AHLs) enable certain Gram‑negative bacteria to respond to environmental changes through behaviours, such as biofilm formation and flagellar movement. The present study aimed to identify Acinetobacter baumannii AHLs and assess their influence on antibiotic resistance. A clinical isolate of A. baumannii strain S (AbS) was collected from the wound of a burn patient and high‑performance liquid chromatography and tandem quadrupole or quadrupole time‑of‑flight high‑resolution mass spectrometry was used to identify AbS AHLs...
June 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28486994/the-emergence-of-a-novel-sequence-type-of-mdr-acinetobacter-baumannii-from-the-intensive-care-unit-of-an-egyptian-tertiary-care-hospital
#18
Doaa Mohammad Ghaith, Mai Mahmoud Zafer, Mohamed Hamed Al-Agamy, Essam J Alyamani, Rayan Y Booq, Omar Almoazzamy
BACKGROUND AND AIM OF WORK: Acinetobacter baumannii is known for nosocomial outbreaks worldwide. In this study, we aimed to investigate the antibiotic susceptibility patterns and the clonal relationship of A. baumannii isolates from the intensive care unit (ICU) of an Egyptian hospital. METHODS: In the present study, 50 clinical isolates of multidrug resistant (MDR)-A. baumannii were obtained from patients admitted into the ICU from June to December 2015. All isolates were analyzed for antimicrobial susceptibilities...
May 10, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28448572/clonality-virulence-determinants-and-profiles-of-resistance-of-clinical-acinetobacter-baumannii-isolates-obtained-from-a-spanish-hospital
#19
Elias Dahdouh, Rosa Gómez-Gil, Sonsoles Pacho, Jesús Mingorance, Ziad Daoud, Monica Suárez
INTRODUCTION: Acinetobacter baumannii is a nosocomial pathogen that is showing increasing rates of carbapenem resistance. Multi-Drug Resistant (MDR) International Clones (ICs), associated with certain oxacillinases, are being reported globally. This organism also harbors numerous virulence determinants. In this study, we aim at characterizing A. baumannii isolated from a Spanish hospital in terms of antimicrobial susceptibility, clonality, carbapenemase genes harbored, and virulence determinants expressed...
2017: PloS One
https://www.readbyqxmd.com/read/28445009/evaluation-of-six-phenotypic-methods-for-the-detection-of-carbapenemases-in-gram-negative-bacteria-with-characterized-resistance-mechanisms
#20
Kunling Sun, Xiuyu Xu, Jinrong Yan, Liping Zhang
BACKGROUND: We compared the performance of the modified Hodge test (MHT), Triton Hodge test (THT), Carba NP test (CNPt), simplified Carba NP test (CNPt-direct), blue-Carba NP test (BCT), and carbapenem inactivation method (CIM) for rapid and accurate carbapenemase detection. METHODS: The methods were evaluated by using 256 gram-negative isolates, including 197 Enterobacteriaceae (79 Enterobacter spp., 74 Klebsiella spp., 33 Escherichia coli, 10 Citrobacter spp., and 1 Serratia marcescens), 51 Acinetobacter baumannii, and 8 Pseudomonas aeruginosa strains...
July 2017: Annals of Laboratory Medicine
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