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https://www.readbyqxmd.com/read/29148877/characterization-and-horizontal-transfer-of-antimicrobial-resistance-genes-and-integrons-in-bacteria-isolated-from-cooked-meat-products-in-china
#1
Tao Yu, Xiaobing Jiang, Yu Liang, Yanping Zhu, Jinhe Tian, Hao Ying, Xuannian Wang, Lei Shi
The aim of this study was to investigate antimicrobial resistance and the presence and transferability of corresponding resistance genes and integrons in bacteria isolated from cooked meat samples in the People's Republic of China. A total of 150 isolates (22 species belonging to 15 genera) were isolated from 49 samples. Resistance of these isolates to antimicrobials was commonly observed; 42.7, 36.0, and 25.3% of the isolates were resistant to tetracycline, streptomycin, and ampicillin, respectively. Multidrug resistance was observed in 41 (27...
November 17, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/29147117/colistin-resistance-of-pseudomonas-aeruginosa-isolated-from-snakes-in-taiwan
#2
Po-Yu Liu, Ling-Ling Weng, Shu-Ying Tseng, Chou-Chen Huang, Ching-Chang Cheng, Yan-Chiao Mao, Kwong-Chung Tung
This study included fifty-eight isolates of P. aeruginosa from the oral cavity of snakes that were recruited from clinical cases, captive and wild snakes. The minimum inhibitory concentrations (MICs) for the determination of susceptibility were identified by the broth microdilution method. Polymerase chain reaction (PCR) was employed to detect β-lactamases genes. With regard to antipseudomonal antibiotics, the lowest nonsusceptible rates were in aztreonam (15%), piperacillin/tazobactam (12%), and amikacin (9%)...
2017: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/29138113/prevalence-of-extended-spectrum-beta-lactamase-and-carbapenemase-encoding-genes-in-poultry-feces-from-algeria-and-marseille-france
#3
Chabou Selma, Leulmi Hamza, Davoust Bernard, Aouadi Atef, Jean-Marc Rolain
OBJECTIVE: The aim of this study was to evaluate the prevalence of extended-spectrum beta lactamase (ESBLs) and carbapenemase-encoding genes in poultry from Algeria and Marseille, France. MATERIALS AND METHODS: The samples consisted of feces from broilers from France and Algeria between 2014 and 2015. DNA extraction and quantitative PCR were performed on 833 fecal samples to screen for the presence of ESBLs (TEM, SHV, CTX-M) and carbapenemase-encoding genes (NDM, OXA48, OXA58)...
November 11, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29125052/prevalence-of-extended-spectrum-beta-lactamase-and-carbapenemase-producing-bloodstream-isolates-of-klebsiella-pneumoniae-in-a-tertiary-care-hospital
#4
Henrietta Abodakpi, Kai-Tai Chang, Ana María Sánchez Díaz, Rafael Cantón, Todd M Lasco, Katrina Chan, Amelia K Sofjan, Vincent H Tam
To improve prescribing of empiric therapy, the local molecular epidemiology of extended-spectrum beta-lactamases (ESBLs) and Klebsiella pneumoniae carbapenemases (KPCs) in bloodstream isolates of K. pneumoniae were evaluated. Isolates resistant to third generation cephalosporins were screened phenotypically for ESBLs and carbapenemases, and subsequently confirmed by PCR for the presence of ESBL (blaTEM, blaSHV and blaCTX-M) and carbapenemase (blaKPC, blaVIM, blaNDM and blaOXA-48) genes. Hydrolytic activity (functional gene expression) was quantified using a nitrocefin degradation assay and correlated to ceftazidime or meropenem MIC...
November 10, 2017: Journal of Chemotherapy
https://www.readbyqxmd.com/read/29109700/exploring-the-genome-and-phenotype-of-multi-drug-resistant-klebsiella-pneumoniae-of-clinical-origin
#5
João Anes, Daniel Hurley, Marta Martins, Séamus Fanning
Klebsiella pneumoniae is an important nosocomial pathogen with an extraordinary resistant phenotype due to a combination of acquired resistant-elements and efflux mechanisms. In this study a detailed molecular characterization of 11 K. pneumoniae isolates of clinical origin was carried out. Eleven clinical isolates were tested for their susceptibilities, by disk diffusion and broth microdilution and interpreted according to CLSI guidelines. Efflux activity was determined by measuring the extrusion of ethidium bromide and biofilm formation was assessed following static growth in Müeller-Hinton and minimal media M9 broths at two temperatures and time points...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29107607/emergence-and-mechanism-of-carbapenem-resistant-escherichia-coli-in-henan-china-2014
#6
Wen-Juan Liang, Hui-Ying Liu, Guang-Cai Duan, Yong-Xin Zhao, Shuai-Yin Chen, Hai-Yan Yang, Yuan-Lin Xi
The emergence and dissemination of carbapenem-resistant Escherichia coli (E. coli) strains is a main risk for global public health, but little is known of carbapenemase producing E. coli in Henan, China. The study was undertaken to investigate the prevalence and mechanism of carbapenem-resistant E. coli strains in a hospital in Xinxiang, Henan, China, 2014. A total of 5 carbapenemase-producing E. coli strains were screened from 1014 isolates. We found that they were all resistant to meropenem and imipenem. Amikacin showed the best sensitivity, with gentamicin coming up next...
October 26, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/29077916/comparison-of-prevalence-phenotype-and-antimicrobial-resistance-of-salmonella-serovars-isolated-from-turkeys-in-taiwan
#7
J-C Yeh, C-L Chen, C-S Chiou, D-Y Lo, J-C Cheng, H-C Kuo
Salmonella spp. is a foodborne pathogen that causes zoonotic disease worldwide. The aim of this study was to investigate the prevalence of antimicrobial resistance of Salmonella isolated from turkey farms in Taiwan. During the past 2 yr, 243 strains of Salmonella were isolated from 2,040 samples (11.9%) from turkey farms, including 32.5% (52/160) from the intestines of 12-day-old turkey poults, 14.2% (119/840) from feces collected from the turkey growing periods, and 6.9% (72/1,040) from finishing periods. S...
October 25, 2017: Poultry Science
https://www.readbyqxmd.com/read/29076938/gut-colonization-with-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-may-increase-disease-activity-in-biologic-naive-outpatients-with-ulcerative-colitis-an-interim-analysis
#8
Vita Skuja, Aleksejs Derovs, Katrina Pekarska, Dace Rudzite, Elvira Lavrinovica, Linda Piekuse, Inga Kempa, Zane Straume, Jelena Eglite, Aivars Lejnieks, Angelika Krumina, Rami Eliakim
BACKGROUND: Certain Enterobacteriaceae strains have been associated with the development of ulcerative colitis (UC). Extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae are the most commonly found multi-drug-resistant (MDR) bacteria colonizing the gut in UC patients and might trigger a more severe disease activity in UC patients. OBJECTIVE: The aim of this study was to evaluate whether disease activity is higher in UC patients with gut colonization with ESBL-producing Enterobacteriaceae...
October 25, 2017: European Journal of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/29075329/occurrence-of-salmonella-enterica-and-escherichia-coli-in-raw-chicken-and-beef-meat-in-northern-egypt-and-dissemination-of-their-antibiotic-resistance-markers
#9
Amira A Moawad, Helmut Hotzel, Omnia Awad, Herbert Tomaso, Heinrich Neubauer, Hafez M Hafez, Hosny El-Adawy
BACKGROUND: The global incidence of foodborne infections and antibiotic resistance is recently increased and considered of public health concern. Currently, scarcely information is available on foodborne infections and ESBL associated with poultry and beef meat in Egypt. METHODS: In total, 180 chicken and beef meat samples as well as internal organs were collected from different districts in northern Egypt. The samples were investigated for the prevalence and antibiotic resistance of Salmonella enterica serovars and Escherichia coli...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/29064348/the-activity-of-fosfomycin-against-extended-spectrum-beta-lactamase-producing-isolates-of-enterobacteriaceae-recovered-from-urinary-tract-infections-a-single-center-study-over-a-period-of-12-years
#10
Parisa Aris, Mohammad Ali Boroumand, Mohammad Rahbar, Masoumeh Douraghi
Despite global efforts to tackle resistance in extended-spectrum beta-lactamase (ESBL)-producing isolates via old antibiotics, there are limited data on the efficacy of fosfomycin-an old oral antibiotic-against Enterobacteriaceae in the Middle East. The purpose of this study was to evaluate the in vitro activity of fosfomycin against urinary ESBL-producing isolates of Enterobacteriaceae. Between 2004 and 2015, 363 isolates of ESBL-producing Enterobacteriaceae were recovered from high-risk patients suffering from cardiac disorders and were subjected to polymerase chain reaction using specific primers for the blaTEM, blaSHV, and blaCTX-M genes...
October 24, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29063811/emergence-of-colistin-resistance-in-enterobacter-aerogenes-from-croatia
#11
Branka Bedenić, Mirna Vranić-Ladavac, Carolina Venditti, Arjana Tambić-Andrašević, Nada Barišić, Marija Gužvinec, Natalie Karčić, Nicola Petrosillo, Ranko Ladavac, Antonino di Caro
A colistin-resistant Enterobacter aerogenes [study code 12264] was isolated from the tracheal aspirate of a 71-year-old male patient in the General Hospital [GH] in Pula, Croatia. The patient was previously treated in University Hospital Centre in Rijeka with colistin in order to eradicate Acinetobacter baumannii isolate, susceptible only to colistin and tigecycline. Genes encoding ESBLs [blaTEM, blaSHV, blaCTX-M, blaPER-1] were screened by PCR. The strain was shown to possess blaCTX-M-15 and blaTEM-1 genes...
October 24, 2017: Journal of Chemotherapy
https://www.readbyqxmd.com/read/29062330/molecular-screening-of-antibiotic-resistant-determinants-among-multidrug-resistant-clinical-isolates-of-proteus-mirabilis-from-southwest-nigeria
#12
Olumuyiwa Samuel Alabi, Nuno Mendonça, Olufemi Ezekiel Adeleke, Gabriela Jorge da Silva
BACKGROUND: Globally, and particularly in developing countries, the menace of anti-microbial resistance is an accelerating problem. In Nigeria, increase in bacterial resistance has been phenotypically established but due to high cost, few molecular studies have been reported. OBJECTIVES: This study screened for presence of transferable resistance genes and mobile genetic elements (MGEs) such as integron among multi-drug resistant (MDR) P. mirabilis. METHODS: A total of 108 P...
June 2017: African Health Sciences
https://www.readbyqxmd.com/read/29058340/molecular-typing-and-occurrence-of-beta-lactam-resistance-genes-of-shigella-sonnei-strains-isolated-during-1983-2014-in-the-s%C3%A3-o-paulo-state-in-brazil
#13
Amanda Ap Seribelli, Miliane R Frazão, Marta I Cazentini Medeiros, Eliana G Stehling, Juliana P Falcão
Shigella sonnei is generally associated with dysentery in developed countries; but it has recently emerged in developing countries. Specifically, in Brazil there are few published studies that molecularly characterized this species. The aims of this study were to analyze the efficacy of the MLVA typing method, to study the phylogeny by MLST and to assess the presence of some beta-lactam resistance genes in S. sonnei strains isolated from human diarrhoeic faeces in the São Paulo State in Brazil between 1983-2014...
October 23, 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/29050976/evolution-in-situ-of-ari-a-in-pb2-1-a-type-1-incc-plasmid-recovered-from-klebsiella-pneumoniae-and-stability-of-tn4352b
#14
Christopher J Harmer, Ruth M Hall
The IncC plasmid pB2-1, from a Klebsiella pneumoniae isolate recovered in Brisbane prior to 1995, belongs to a subtype of type 1 IncC plasmids, here designated type 1a, that includes those carrying carbapenem resistance genes such as blaNDM and blaKPC. pB2-1 carries a 2358bp deletion in the rhs1 gene found in four other type 1a IncC plasmids. pB2-1 confers resistance to ampicillin, gentamicin, kanamycin, neomycin, tobramycin, sulfamethoxazole, tetracycline and trimethoprim. It transferred at a frequency of 4...
October 16, 2017: Plasmid
https://www.readbyqxmd.com/read/29039715/antibiotic-resistance-in-salmonella-from-retail-foods-of-animal-origin-and-its-association-with-disinfectant-and-heavy-metal-resistance
#15
Wenwen Deng, Yuan Quan, Shengzhi Yang, Lijuan Guo, Xiuli Zhang, Shuliang Liu, Shujuan Chen, Kang Zhou, Li He, Bei Li, Yunfu Gu, Shaohua Zhao, Likou Zou
This study aims to demonstrate the antibiotic resistance and its association with disinfectant and heavy metal resistance in 152 Salmonella isolates recovered from retail foods of animal origins. Susceptibility testing demonstrated that 92.8% isolates were resistant to at least one antibiotic, and the resistance was highest to oxytetracycline (80.9%), followed by trimethoprim (64.5%), amoxicillin (28.9%), ampicillin (28.3%), levofloxacin (21.7%), ciprofloxacin (16.4%), and gentamicin (10.5%), respectively. The blaTEM and tetA genes (44...
October 17, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29038771/detection-of-antibiotic-resistance-genes-in-the-feces-of-young-adult-japanese
#16
Yuji Ohashi, Tomohiko Fujisawa
Antibiotic resistance genes in the feces of healthy young adult Japanese were analyzed with polymerase chain reaction using specific primers. Antibiotic resistance genes against macrolides (ermB, ermF, ermX, and mefA/E), tetracyclines (tetW, tetQ, tetO, and tetX), β-lactam antibiotics (blaTEM ), and streptomycin (aadE) were detected in more than 50% of subjects. These antibiotic resistance genes are likely widespread in the large intestinal bacteria of young adult Japanese.
2017: Bioscience of Microbiota, Food and Health
https://www.readbyqxmd.com/read/29031406/prevalence-of-antibiotic-resistance-genes-from-effluent-of-coastal-aquaculture-south-korea
#17
Hyun Min Jang, Young Beom Kim, Sangki Choi, Yunho Lee, Seung Gu Shin, Tatsuya Unno, Young Mo Kim
The wide use of antibiotics in aquaculture for prophylactic and therapeutic purposes can potentially lead to the prevalence of antibiotic resistance genes (ARGs). This study reports for the first time the profile of ARGs from effluents of coastal aquaculture located in South Jeolla province and Jeju Island, South Korea. Using quantitative PCR (qPCR), twenty-two ARGs encoding tetracycline resistance (tetA, tetB, tetD, tetE, tetG, tetH, tetM, tetQ, tetX, tetZ, tetBP), sulfonamide resistance (sul1, sul2), quinolone resistance (qnrD, qnrS, aac(6')-Ib-cr), β-lactams resistance (blaTEM, blaCTX, blaSHV), macrolide resistance (ermC), florfenicol resistance (floR) and multidrug resistance (oqxA) and a class 1 integrons-integrase gene (intI1) were quantified...
October 12, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29022829/migratory-white-stork-ciconia-ciconia-a-potential-vector-of-the-oxa-48-producing-escherichia-coli-st38-clone-in-algeria
#18
Amira Bouaziz, Lotfi Loucif, Ammar Ayachi, Karima Guehaz, Esma Bendjama, Jean-Marc Rolain
The emergence of carbapenemase-producing Enterobacteriaceae is of great concern to public health worldwide. The aim of this study was to screen for the presence of carbapenemase-producing Enterobacteriaceae in white stork (Ciconia ciconia) migratory bird stools, and to investigate their molecular support on β-lactamase production. In March 2015, 32 fecal samples of white stork were collected in the Commune of El Madher Wilaya de Batna, in eastern Algeria. Samples were subjected to selective isolation of carbapenem-resistant Enterobacteriaceae...
October 12, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29017452/whole-genome-sequencing-for-the-molecular-characterization-of-carbapenem-resistant-klebsiella-pneumoniae-strains-isolated-at-the-italian-asst-fatebenefratelli-sacco-hospital-2012-2014
#19
Sara Giordana Rimoldi, Bernardina Gentile, Cristina Pagani, Annamaria Di Gregorio, Anna Anselmo, Anna Maria Palozzi, Antonella Fortunato, Valentina Pittiglio, Anna Lisa Ridolfo, Maria Rita Gismondo, Giuliano Rizzardini, Florigio Lista
BACKGROUND: The emergence of carbapenem-resistant Klebsiella pneumoniae strains is threatening antimicrobial treatment. METHODS: Sixty-eight carbapenemase-producing K. pneumoniae strains isolated at Luigi Sacco University Hospital-ASST Fatebenefratelli Sacco (Milan, Italy) between 2012 and 2014 were characterised microbiologically and molecularly. They were tested for drug susceptibility and carbapenemase phenotypes, investigated by means of repetitive extra-genic palindromic polymerase chain reaction (REP-PCR), and fully sequenced by means of next-generation sequencing for the in silico analysis of multi-locus sequence typing (MLST), their resistome, virulome and plasmid content, and their core single nucleotide polymorphism (SNP) genotypes...
October 10, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28987805/emergence-of-carbapenem-resistant-escherichia-coli-isolates-producing-blandm-and-blaoxa-48-like-carried-on-inca-c-and-incl-m-plasmids-at-two-iranian-university-hospitals
#20
Hamid Solgi, Christian G Giske, Farzad Badmasti, Shadi Aghamohammad, Seyed Asghar Havaei, Shahram Sabeti, Kamyar Mostafavizadeh, Fereshteh Shahcheraghi
The emergence of carbapenem resistance among Escherichia coli is a serious threat to public health. The objective of this study was to investigate resistance genes and clonality of carbapenem resistant E. coli in Iran. Between February 2015 and July 2016, a total of 32 non-duplicate E. coli isolates that were ertapenem resistant or intermediate (R/I-ETP) were collected from patient clinical or surveillance cultures (rectal swabs) at two university hospitals. Resistance genes were identified by PCR and sequencing...
October 4, 2017: Infection, Genetics and Evolution
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