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https://www.readbyqxmd.com/read/28922058/evolution-of-regions-containing-antibiotic-resistance-genes-in-fii-2-fib-1-colv-colla-virulence-plasmids
#1
Robert A Moran, Ruth M Hall
Three ColV virulence plasmids carrying antibiotic resistance genes were assembled from draft genome sequences of commensal ST95, ST131, and ST2705 Escherichia coli isolates from healthy Australians. Plasmids pCERC4, pCERC5, and pCERC9 include almost identical backbones containing FII-2 and FIB-1 replicons and the conserved ColV virulence region with an additional ColIa determinant. Only pCERC5 includes a complete, uninterrupted F-like transfer region and was able to conjugate. pCERC5 and pCERC9 contain Tn1721, carrying the tet(A) tetracycline resistance determinant in the same location, with Tn2 (blaTEM; ampicillin resistance) interrupting the Tn1721 in pCERC5...
September 18, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28919689/beta-lactamase-antimicrobial-resistance-in-klebsiella-and-enterobacter-species-isolated-from-healthy-and-diarrheic-dogs-in-andhra-pradesh-india
#2
N Mohammad Sharif, B Sreedevi, R K Chaitanya, D Sreenivasulu
AIM: The aim of this study was to characterize beta-lactamase antimicrobial resistance in Klebsiella and Enterobacter species isolated from healthy and diarrheic dogs in Andhra Pradesh. MATERIALS AND METHODS: A total of 136 rectal swabs were collected from healthy (92) and diarrheic (44) dogs, bacteriological cultured for Klebsiella and Enterobacter growth and screened for beta-lactamase antimicrobial resistance phenotypically by disc diffusion method and genotypically by polymerase chain reaction targeting blaTEM, blaSHV, blaOXA, blaCTX-M Group 1, 2, blaAmpC, blaACC, and blaMOX genes...
August 2017: Veterinary World
https://www.readbyqxmd.com/read/28912764/resistance-to-antibiotics-biocides-preservatives-and-metals-in-bacteria-isolated-from-seafoods-co-selection-of-strains-resistant-or-tolerant-to-different-classes-of-compounds
#3
José L Romero, María J Grande Burgos, Rubén Pérez-Pulido, Antonio Gálvez, Rosario Lucas
Multi-drug resistant bacteria (particularly those producing extended-spectrum β-lactamases) have become a major health concern. The continued exposure to antibiotics, biocides, chemical preservatives, and metals in different settings such as the food chain or in the environment may result in development of multiple resistance or co-resistance. The aim of the present study was to determine multiple resistances (biocides, antibiotics, chemical preservatives, phenolic compounds, and metals) in bacterial isolates from seafoods...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28900355/distribution-of-blatem-blashv-and-blactx-m-genes-among-esbl-producing-p-aeruginosa-isolated-from-qazvin-and-tehran-hospitals-iran
#4
REVIEW
A Peymani, T Naserpour-Farivar, E Zare, K H Azarhoosh
INTRODUCTION: Pseudomonas aeruginosa is as an important opportunistic human pathogen, which is associated with several clinical infections that are usually difficult to treat because of resistance to multiple antimicrobials. The production of extendedspectrum ß-lactamases (ESBLs) is an important mechanism of ß-lactam resistance. The aims of this study were to determine the prevalence of ESBLs, antimicrobial susceptibility, and to detect the blaTEM, blaSHV, and blaCTX-M genes. METHODS: In this study, carried out from March 2013 to December 2014, 266 P...
June 2017: Journal of Preventive Medicine and Hygiene
https://www.readbyqxmd.com/read/28892891/distribution-of-class-d-carbapenemase-and-extended-spectrum-%C3%AE-lactamase-genes-among-acinetobacter-baumannii-isolated-from-burn-wound-and-ventilator-associated-pneumonia-infections
#5
Maryam Mohammadi, Setareh Soroush, Somayeh Delfani, Iraj Pakzad, Abolfazl Abbaszadeh, Mahmoud Bahmani, Lidija Bogdanovic, Morovat Taherikalani
INTRODUCTION: Resistance to Acinetobacter baumannii is dramatically on the rise in Iran. Therefore, it is important to study resistance pattern among Acinetobacter isolates which is a common cause of nosocomial infections. AIM: To investigate antibiotic resistance patterns and the role of resistant genes and biofilm formation in the induction of resistance among Acinetobacter baumannii isolated from burn wound and ventilator associated pneumonia infections. MATERIALS AND METHODS: Total 103 isolates such as 33 burn samples from Rasool Akram Hospital and 70 isolates from ventilated patients in Shahid Motahhari Hospital were identified with A...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28887199/temporal-evolution-of-polymyxin-b-resistant-klebsiella-pneumoniae-clones-recovered-from-blood-cultures-in-a-teaching-hospital-during-a-7-year-period
#6
Graziela Braun, Rodrigo Cayô, Adriana Pereira Matos, Juliane de Mello Fonseca, Ana Cristina Gales
The polymyxins have become one of the last resorts to treat serious infections caused by KPC-2-producing Klebsiella pneumoniae worldwide. However, the increase of polymyxin consumption has favored the emergence of resistance to these compounds. In this study we observed an increase in the polymyxin B resistance rates from 0 to 30.6% among 224 K. pneumoniae isolates recovered from blood cultures between 2009 and 2015. Only gentamicin, tigecycline and fosfomycin remained active against the polymyxin B-resistant K...
September 5, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28878891/epidemic-characterization-and-molecular-genotyping-of-shigella-flexneri-isolated-from-calves-with-diarrhea-in-northwest-china
#7
Zhen Zhu, Mingze Cao, Xuzheng Zhou, Bing Li, Jiyu Zhang
BACKGROUND: The widespread presence of antibiotics resistance genes in pathogenic bacteria can cause enormous problems. Food animals are one of the main reservoirs of intestinal pathogens that pose a potential risk to human. Analyzing the epidemiological characteristics and resistance patterns of Shigella flexneri in calves is necessary for animal and human health. METHODS AND RESULTS: A total of 54 Shigella flexneri isolates, including six serotypes (1a, 2a, 2b, 4a, 6 and Xv), were collected from 837 fecal samples obtained from 2014 to 2016...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28876169/ndm-1-hazard-in-the-balkan-states-evidence-of-the-first-outbreak-of-ndm-1-producing-klebsiella-pneumoniae-in-bulgaria
#8
Encho Savov, Lida Politi, Nicholas Spanakis, Angelina Trifonova, Elena Kioseva, Athanasios Tsakris
New Delhi MBL (NDM) carbapenemase-producing Klebsiella pneumoniae has become one of the most concerning multidrug-resistant pathogens. The Balkan counties are considered a reservoir for the spread of such strains based on several reports documenting NDM infections after hospitalization in this region. Nevertheless, NDM-producing K. pneumoniae have been only occasionally documented from Balkans. The current study documents the first polyclonal outbreak caused by NDM-1-producing K. pneumoniae in Bulgaria. From July 2015 to April 2016, all 25 single-patient carbapenem-nonsusceptible K...
September 6, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28876168/complete-sequence-of-pcy-ctx-a-plasmid-carrying-a-phage-like-region-and-an-isecp1-mediated-tn2-element-from-enterobacter-cloacae
#9
Zhenbo Xu, Jinhong Xie, Ling Yang, Dingqiang Chen, Brian M Peters, Mark E Shirtliff
A plasmid pCY-CTX carrying a phage-like backbone from an extensively drug-resistant Enterobacter cloacae strain Guangzhou-ECL001 (previously known as CY01) was identified in this study. By Illumina MiSeq 2 × 250-bp paired-end sequencing, de novo assembly, and PCR, full sequence of pCY-CTX was obtained. Plasmid pCY-CTX was a circular plasmid with a length of 116,700 bp, harboring 136 putative open reading frames with the average G + C content of 50.8%. The backbone of pCY-CTX showed high identity to previously reported phage-like plasmid pHCM2 and phage SSU5...
September 6, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28870291/genomic-characterization-of-an-extensively-drug-resistance-salmonella-enterica-serotype-indiana-strain-harboring-blandm-1-gene-isolated-from-a-chicken-carcass-in-china
#10
Wei Wang, Zixin Peng, Zulqarnain Baloch, Yujie Hu, Jin Xu, Wenhui Zhang, Séamus Fanning, Fengqin Li
OBJECTIVES: The objective of this study was to genetically characterize the antimicrobial resistance mechanisms of Salmonella enterica serotype Indiana C629 isolated from a chicken carcass in China in 2014. METHODS: Antimicrobial susceptibility against a panel of 23 antimicrobial agents was carried out on Salmonella enterica serotype Indiana C629 and assessed according to CLSI standards. Whole-genome sequencing of this isolate was conducted to obtain the complete genome of S...
November 2017: Microbiological Research
https://www.readbyqxmd.com/read/28867789/multiple-antibiotic-resistant-extended-spectrum-%C3%AE-lactamase-esbl-producing-enterobacteria-in-fresh-seafood
#11
Asem Sanjit Singh, Manjusha Lekshmi, Sreepriya Prakasan, Binaya Bhusan Nayak, Sanath Kumar
Members of the family Enterobacteriaceae include several human pathogens that can be acquired through contaminated food and water. In this study, the incidence of extended spectrum β-lactamase (ESBL)-producing enterobacteria was investigated in fresh seafood sold in retail markets. The ESBL-positive phenotype was detected in 169 (78.60%) isolates, with Escherichia coli being the predominant species (53), followed by Klebsiella oxytoca (27), and K. pneumoniae (23). More than 90% of the isolates were resistant to third generation cephalosporins, cefotaxime, ceftazidime, and cefpodoxime...
August 30, 2017: Microorganisms
https://www.readbyqxmd.com/read/28863344/elimination-of-antibiotic-resistance-bacterium-and-its-associated-dissociative-blatem-1-and-aac-3-ii-antibiotic-resistance-genes-in-aqueous-system-via-photoelectrocatalytic-process
#12
Qi Jiang, Hongliang Yin, Guiying Li, Hongli Liu, Taicheng An, Po Keung Wong, Huijun Zhao
The ubiquity of antibiotic-resistance bacteria (ARB) and antibiotic-resistance genes (ARGs) in various environmental matrices is a potential threat to human and ecological health. Therefore, the inactivation of ARB E. coli S1-23 and the elimination of its associated ARGs, blaTEM-1 and aac(3)-II, were investigated using the photoelectrocatalytic (PEC) process. Results indicate that the ARB E. coli S1-23 (1 × 10(8) cfu mL(-1)) and its ARGs (extracellular and intracellular) could be fully inactivated within 10 and 16 h PEC treatment, respectively...
August 23, 2017: Water Research
https://www.readbyqxmd.com/read/28831220/serotyping-antibiotic-susceptibility-and-virulence-genes-screening-of-escherichia-coli-isolates-obtained-from-diarrheic-buffalo-calves-in-egyptian-farms
#13
Ashraf S Hakim, Shimaa T Omara, Sohier M Syame, Ehab A Fouad
AIM: In Egypt as in many other countries, river water buffalo (Bubalus bubalis) is considered an important source of high-quality milk and meat supply. The objective of this study was to investigate serotypes, virulence genes, and antibiotic resistance determinants profiles of Escherichia coli isolated from buffalo at some places in Egypt; noticibly, this issue was not discussed in the country yet. MATERIALS AND METHODS: A number of 58 rectal samples were collected from diarrheic buffalo calves in different regions in Egypt, and bacteriological investigated for E...
July 2017: Veterinary World
https://www.readbyqxmd.com/read/28829687/prevalence-of-ctx-m-producing-klebsiella-spp-in-broiler-kuroiler-and-indigenous-poultry-in-west-bengal-state-india
#14
Achintya Mahanti, Pratik Ghosh, Indranil Samanta, Siddhartha Narayan Joardar, Samiran Bandyopadhyay, Debaraj Bhattacharyya, Jaydeep Banerjee, Subhasis Batabyal, Tapas Kumar Sar, Tapan Kumar Dutta
This study was undertaken to detect the prevalence of CTX-M-producing Klebsiella spp. in healthy broiler, indigenous, and kuroiler birds reared in West Bengal (India) during November 2014-February 2015. In addition to CTX-M gene, the study was also conducted to reveal the occurrence of other β-lactamase and class I integron genes in Klebsiella spp. isolates along with their clonal relationship. A total of 321 cloacal swabs from healthy broiler, indigenous, and kuroiler birds were collected from different places of West Bengal, India...
August 22, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28812563/multicopy-plasmids-potentiate-the-evolution-of-antibiotic-resistance-in-bacteria
#15
Alvaro San Millan, Jose Antonio Escudero, Danna R Gifford, Didier Mazel, R Craig MacLean
Plasmids are thought to play a key role in bacterial evolution by acting as vehicles for horizontal gene transfer, but the role of plasmids as catalysts of gene evolution remains unexplored. We challenged populations of Escherichia coli carrying the blaTEM-1 β-lactamase gene on either the chromosome or a multicopy plasmid (19 copies per cell) with increasing concentrations of ceftazidime. The plasmid accelerated resistance evolution by increasing the rate of appearance of novel TEM-1 mutations, thereby conferring resistance to ceftazidime, and then by amplifying the effect of TEM-1 mutations due to the increased gene dosage...
November 7, 2016: Nature ecology & evolution
https://www.readbyqxmd.com/read/28803933/phage-in-urban-wastewater-have-the-potential-to-disseminate-antibiotic-resistance
#16
Gayathri Upeksha Gunathilaka, Varun Tahlan, Abdullah Ibn Mafiz, Manisha Polur, Yifan Zhang
A total of 29 Escherichia coli phage were isolated from wastewater samples collected from an urban wastewater treatment plant (WWTP) and characterized by host range determination, Transmission Electron Microscopy (TEM), antibiotic resistance gene identification, and phage transduction. β-lactam resistance genes, blaCMY, blaTEM, blaSHV, blaCTXM, and blaOXA, were amplified on phage DNA by PCR. Out of nine host range patterns observed, six were able to multiply in three or more indicator strains, including Shiga-toxin producing E...
August 10, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28800411/antimicrobial-resistance-and-resistance-genes-in-salmonella-strains-isolated-from-broiler-chickens-along-the-slaughtering-process-in-china
#17
Yuanting Zhu, Haimei Lai, Likou Zou, Sheng Yin, Chengtao Wang, Xinfeng Han, Xiaolong Xia, Kaidi Hu, Li He, Kang Zhou, Shujuan Chen, Xiaolin Ao, Shuliang Liu
A total of 189 Salmonella isolates were recovered from 627 samples which were collected from cecal contents of broilers, chicken carcasses, chicken meat after cutting step and frozen broiler chicken products along the slaughtering process at a slaughterhouse in Sichuan province of China. The Salmonella isolates were subjected to antimicrobial susceptibility testing to 10 categories of antimicrobial agents using the Kirby-Bauer disk diffusion method. Those antibiotics-resistant isolates were further investigated for the occurrence of resistance genes, the presence of class 1 integron as well as the associated gene cassettes, and the mutations within the gyrA and parC genes...
August 1, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28797325/plasmid-borne-colistin-resistance-gene-mcr-3-in-salmonella-isolates-from-human-infections-denmark-2009-17
#18
Eva Litrup, Kristoffer Kiil, Anette M Hammerum, Louise Roer, Eva M Nielsen, Mia Torpdahl
This report describes one Salmonella isolate harbouring both mcr-1 and mcr-3. We also found nine other Salmonella isolates positive for the plasmid-borne colistin resistance gene, mcr-3. The strains were isolated from patients in Denmark between 2009 and 2017 and five of the patients had travelled to Asia. In addition to mcr-3, all strains were found positive for blaTEM-1, strA, strB, sul2 and tet(A) or tet(B), and most strains were positive for blaCTX-M-55 and qnrS.
August 3, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28780105/short-communication-extended-spectrum-cephalosporin-resistant-escherichia-coli-in-colostrum-from-new-brunswick-canada-dairy-cows-harbor-blacmy-2-and-blatem-resistance-genes
#19
B B Awosile, J T McClure, J Sanchez, J VanLeeuwen, J C Rodriguez-Lecompte, G Keefe, L C Heider
Dairy calves are colonized shortly after birth by multidrug resistant (MDR) bacteria, including Escherichia coli. The role of dairy colostrum fed to calves as a potential source of MDR bacteria resistance genes has not been investigated. This study determined the recovery rate of extended-spectrum cephalosporin-resistant (ESC-R) E. coli in colostrum from cows. The ESC-R E. coli isolates were further investigated to determine their phenotypic antimicrobial resistance pattern and the genes conferring ESC-R. Fresh colostrum was collected from 452 cows from 8 dairy herds in New Brunswick, Canada...
August 2, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28767588/successful-ceftazidime-avibactam-treatment-of-mdr-kpc-positive-klebsiella-pneumoniae-infection-in-a-patient-with-traumatic-brain-injury-a-case-report
#20
Agnese Gugliandolo, Carla Caio, Maria Lina Mezzatesta, Carmela Rifici, Placido Bramanti, Stefania Stefani, Emanuela Mazzon
RATIONALE: Carbapenem-resistant Enterobacteriaceae infections are a serious health care problem, because of the high mortality. Carbapenem resistance is mainly caused by carbapenemases production, including Klebsiella pneumoniae carbapenemase (KPC). Ceftazidime-avibactam is a new cephalosporin/β-lactamase inhibitor combination for the treatment of complicated urinary, intra-abdominal infections, and nosocomial pneumonia caused by gram negative, or other serious gram-negative infections...
August 2017: Medicine (Baltimore)
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