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https://www.readbyqxmd.com/read/28420242/high-prevalence-of-ndm-metallo-%C3%AE-lactamase-among-esbl-producing-escherichia-coli-%C3%A4-solates
#1
Yeliz Çetinkol, Cemal Sandalli, Mustafa Kerem Çalgin, Arzu Altunçekiç Yildirim, Esma Akyildiz, Esin Karaman, Ayşegül Çopur Çiçek
Resistance to β-lactams in Enterobacteriaceae has been increasing worldwide. This study aimed to determine the frequency of β-lactamase genes and antibiotic resistance rates of 140 extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates obtained from urinary tract infection in Ordu Province, Turkey. Isolates were identified by classic methods and by automated system. ESBL production was confirmed by double disk synergy test and antimicrobial susceptibility was investigated by disk diffusion method...
April 19, 2017: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/28412384/molecular-epidemiology-of-penicillinase-producing-neisseria-gonorrhoeae-isolates-in-france
#2
Maité Micaëlo, Agathe Goubard, Guy La Ruche, Erick Denamur, Olivier Tenaillon, Emmanuelle Cambau, Hervé Jacquier, Béatrice Bercot
OBJECTIVES: Characterising the molecular epidemiology of antibiotic resistance is crucial for a better understanding of the evolution and spread of resistance in Neisseria gonorrhoeae. Here, we examine the molecular epidemiology of penicillinase-producing N. gonorrhoeae (PPNG) isolates in France. METHODS: We investigated 176 PPNG isolates collected between 2010 and 2012 by the National Reference Centre in France. Genotyping was performed using the NG-MAST technique, blaTEM genes were Sanger-sequenced, and plasmids were characterized by PCR-typing...
April 13, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28402509/identification-of-is1r-and-is10r-elements-inserted-into-ompk36-porin-gene-of-two-multidrug-resistant-klebsiella-pneumoniae-hospital-strains
#3
Anastasia I Lev, Evgeny I Astashkin, Rima Z Shaikhutdinova, Mikhail E Platonov, Nikolay N Kartsev, Nikolay V Volozhantsev, Olga N Ershova, Edward A Svetoch, Nadezhda K Fursova
Hospital Klebsiella pneumoniae strains (n = 196) were collected in 2012-16 from the patients of Moscow neurosurgical intensive care unit. K. pneumoniae strains were multi drug resistant (MDR) and carried beta-lactamase genes blaSHV (97.4% strains), blaCTX-M (84.7%), blaTEM (56.1%), blaOXA-48-like (49.0%), and blaNDM-1 (one strain), class 1 integrons (43.4% strains) and porin protein ompK36 gene (100% strains). The ompK36 porin protein gene disruption by IS-elements and OmpK36 production loss in two strains were detected in this study...
April 11, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28400764/antimicrobial-resistance-of-salmonella-enterica-serovar-typhimurium-in-shanghai-china
#4
Jinyan Wang, Yongrui Li, Xuebin Xu, Beibei Liang, Fuli Wu, Xiaoxia Yang, Qiuxia Ma, Chaojie Yang, Xiaofeng Hu, Hongbo Liu, Hao Li, Chunyu Sheng, Jing Xie, Xinying Du, Rongzhang Hao, Shaofu Qiu, Hongbin Song
We aimed to analyze the antimicrobial resistance phenotypes and to elucidate the molecular mechanisms underlying resistance to cephalosporins, ciprofloxacin, and azithromycin in Salmonella enterica serovar Typhimurium isolates identified from patients with diarrhea in Shanghai. The isolates showed high rates of resistance to traditional antimicrobials, and 20.6, 12.7, and 5.5% of them exhibited decreased susceptibility to cephalosporins, ciprofloxacin, and azithromycin, respectively. Notably, 473 (84.6%) isolates exhibited multidrug resistance (MDR), including 161 (28...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28379732/prevalence-of-plasmid-mediated-fosfomycin-resistance-gene-fosa3-among-ctx-m-producing-escherichia-coli-isolates-from-chickens-in-china
#5
Wei Jiang, Shuai Men, Linghan Kong, Suzhen Ma, Yongqiang Yang, Yongxiang Wang, Qiwu Yuan, Guangyang Cheng, Wencheng Zou, Hongning Wang
The aim of this study was to investigate the prevalence of fosfomycin resistance gene fosA3 and characterize plasmids harboring fosA3 among CTX-M-producing Escherichia coli from chickens in China. A total of 234 CTX-M-producing E. coli isolates collected from chickens from 2014 to 2016 were screened for the presence of plasmid-mediated fosfomycin resistance genes (fosA, fosA3, and fosC2). Clonal relatedness of fosA3-positive isolates was determined by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST)...
April 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28365323/pathogenic-potentials-of-aeromonas-species-isolated-from-aquaculture-and-abattoir-environments
#6
Isoken H Igbinosa, Abeni Beshiru, Emmanuel E Odjadjare, Collins N Ateba, Etinosa O Igbinosa
The present study elucidated the presence of antibiotics resistance, virulence genes and biofilm potentials among Aeromonas species isolated from abattoir and aquaculture environments in Benin City, Nigeria. A total of 144 wastewater samples were obtained from two independent aquaculture and abattoir environments between May and October 2016. Aeromonas species were isolated on Glutamate Starch Phenol Red (GSP) agar and confirmed using API 20NE kits. Antimicrobial susceptibility profile of the isolates was carried out using standard disc diffusion assay while biofilm potentials were detected by the microtitre plate method and PCR technique was used to detect antibiotics resistance and virulence gene markers...
March 30, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28359833/first-report-in-africa-of-two-clinical-isolates-of-proteus-mirabilis-carrying-salmonella-genomic-island-sgi1-variants-sgi1-pmabb-and-sgi1-w
#7
Ahmed M Soliman, Ashraf M Ahmed, Toshi Shimamoto, Ramadan A El-Domany, Hirofumi Nariya, Tadashi Shimamoto
Two Proteus mirabilis strains, designated PmTAN59 and PmKAF126, were isolated from two different Egyptian cities in 2014 and 2015, respectively. PmTAN59 was isolated from a sputum swab from a pneumonia patient in Tanta University Teaching Hospital. PmKAF126 was isolated from a patient with a diabetic foot infection in a hospital in the city of Kafr El-Sheikh. The two isolates were identified with bacterial small ribosomal RNA (16S rRNA) gene amplification and sequencing and tested for antimicrobial sensitivity with a Kirby-Bauer disk diffusion assay...
March 28, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28344395/characterization-of-salmonella-gallinarum-from-an-outbreak-in-raigarh-chhattisgarh
#8
Chandrahas Sannat, Anil Patyal, Nidhi Rawat, R C Ghosh, D K Jolhe, R K Shende, S D Hirpurkar, Sanjay Shakya
AIM: The present investigation was conducted to isolate and characterize Salmonella Gallinarum from an outbreak of fowl typhoid in layer birds. MATERIALS AND METHODS: Clinically ill and dead layer birds from an outbreak were investigated. History, clinical signs, and postmortem lesions were suggestive of fowl typhoid. Postmortem samples including heart blood, intestinal contents, pieces of ovary, and liver were collected and processed immediately for bacterial culture, serotyping and antibiotic sensitivity tests...
February 2017: Veterinary World
https://www.readbyqxmd.com/read/28339845/expanded-spectrum-%C3%AE-lactamase-producing-escherichia-coli-isolated-from-chickens-with-colibacillosis-in-egypt
#9
D A El-Shazly, S A Nasef, F F Mahmoud, Daniel Jonas
Throughout the world, expanded spectrum β-lactamases (ESBL) are increasing among clinical isolates of Enterobacteriaceae, both in humans and animals. Unfortunately, there is a paucity of data on ESBL or Ampicillin class C β-lactamase (AmpC) in Egypt, although antimicrobial consumption is high in this developing country. This study aims to characterize the resistance mechanisms to expanded spectrum cephalosporins among resistant veterinary Escherichia coli isolates in Egypt. We investigated 50 clinical multi-resistant E...
February 23, 2017: Poultry Science
https://www.readbyqxmd.com/read/28338012/prevalence-of-st1193-clone-and-inci1-st16-plasmid-in-e-coli-isolates-carrying-blactx-m-55-gene-from-urinary-tract-infections-patients-in-china
#10
Liang Xia, Yang Liu, Shu Xia, Timothy Kudinha, Shu-Nian Xiao, Nan-Shan Zhong, Guo-Sheng Ren, Chao Zhuo
To study molecular epidemiology of CTX-M-55-carrying Escherichia coli isolates from urinary tract infections (UTIs) in China. 111 blaCTX-M-55-positive E.coli isolates from UTIs patients in China were studied. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used to analyze the homologies among the strains. Conjugation experiments, S1nuclease PFGE and PCR analysis were performed to characterize plasmids harboring blaCTX-M-55 and their genetic environment. 111 isolates were clustered into 86 individual pulsotypes and three clusters by PFGE...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28337342/first-indian-report-of-incx3-plasmid-carrying-blandm-7-in-escherichia-coli-from-bloodstream-infection-potential-for-rapid-dissemination
#11
N K Devanga Ragupathi, D P Muthuirulandi Sethuvel, R Gajendiran, J L K Daniel, K Walia, B Veeraraghavan
Enterobacteriaceae with blaNDM-7 is only infrequently observed. Self-transmissible plasmids carrying the blaNDM gene increase the dissemination of carbapenem resistance in developing countries. This study investigates the whole genome sequence of a blaNDM-7-positive Escherichia coli. The isolate was an extended-spectrum β-lactamase producer by combined disc diffusion test and carbapenemase producer by CarbaNP method. Sequencing results revealed the isolate as E. coli ST-167 with IncX3 plasmid carrying blaNDM-7 in addition to blaTEM-1 and blaCMY-42 genes...
May 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/28323856/risk-factors-for-esbl-producing-escherichia-coli-on-pig-farms-a-longitudinal-study-in-the-context-of-reduced-use-of-antimicrobials
#12
Wietske Dohmen, Alejandro Dorado-García, Marc J M Bonten, Jaap A Wagenaar, Dik Mevius, Dick J J Heederik
The presence of extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-E. coli) in food animals is a public health concern. This study aimed to determine prevalence of ESBL-E. coli on pig farms and to assess the effect of reducing veterinary antimicrobial use (AMU) and farm management practices on ESBL-E. coli occurrence on pig farms. During 2011-2013, 36 Dutch conventional pig farms participated in a longitudinal study (4 sampling times in 18 months). Rectal swabs were taken from 60 pigs per farm and pooled per 6 pigs within the same age category...
2017: PloS One
https://www.readbyqxmd.com/read/28320725/occurrence-of-acquired-16s-methyltransferase-mediated-aminoglycoside-resistance-in-clinical-isolates-of-enterobacteriaceae-within-a-tertiary-referral-hospital-of-northeast-india
#13
Jayalaxmi Wangkheimayum, Deepjyoti Paul, Debadatta Dhar, Rajlakshmi Nepram, Shiela Chetri, Deepshikha Bhowmik, Atanu Chakravarty, Amitabha Bhattacharjee
Objectives: Methylation of ribosomal target leads to a high level of resistance towards the entire clinically relevant aminoglycoside antibiotics, so early detection of these resistant determinants will help to reduce the treatment failure as well as will lessen the dissemination rate. Here, we characterize different 16s methyltransferase responsible for aminoglycoside resistance and their epidemiological background in clinical isolates of enterobacteriaceae in a tertiary referral hospital of India.Methods: All aminoglycoside resistant isolates were screened for different 16s methyltransferase by PCR assay and incompatibility typing of the conjugable plasmid harboring resistant genes was determined by PCR based replicon typing...
March 20, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28304216/characterizing-the-multidrug-resistance-of-non-o157-shiga-toxin-producing-escherichia-coli-isolates-from-cattle-farms-and-abattoirs
#14
Carrie-Ann Kennedy, Séamus Fanning, Maria Karczmarczyk, Brian Byrne, Áine Monaghan, Declan Bolton, Torres Sweeney
Non-O157 Shiga toxin-producing Escherichia coli (STECs) are not as well characterized as O157 STEC cases, despite their similar prevalence in many countries. Hence, the objective of this study was to investigate the phenotypic and genotypic basis of multidrug resistance (MDR) in non-O157 STEC farm- and abattoir-sourced isolates and assess the potential dissemination of these MDR profiles in vitro. Susceptibility testing to 20 antimicrobials was performed on 146 non-O157 STECs isolated from farm and abattoir environments...
March 17, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28302074/clonality-outer-membrane-proteins-profile-and-efflux-pump-in-kpc-producing-enterobacter-sp-in-brazil
#15
Juliana Ferraz Rosa, Camila Rizek, Ana Paula Marchi, Thais Guimaraes, Lourdes Miranda, Claudia Carrilho, Anna S Levin, Silvia F Costa
BACKGROUND: Carbapenems resistance in Enterobacter spp. has increased in the last decade, few studies, however, described the mechanisms of resistance in this bacterium. This study evaluated clonality and mechanisms of carbapenems resistance in clinical isolates of Enterobacter spp. identified in three hospitals in Brazil (Hospital A, B and C) over 7-year. METHODS: Antibiotics sensitivity, pulsed-field gel electrophoresis (PFGE), PCR for carbapenemase and efflux pump genes were performed for all carbapenems-resistant isolates...
March 17, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28300643/draft-genome-sequence-of-multidrug-resistant-salmonella-enterica-serovar-brancaster-strain-ps01-isolated-from-chicken-meat-malaysia
#16
Pui San Chin, Choo Yee Yu, Geik Yong Ang, Wai-Fong Yin, Kok-Gan Chan
OBJECTIVES: Salmonella spp. represent one of the main diarrhoeal pathogens that are transmitted via the food supply chain. Here we report the draft genome sequence of a multidrug-resistant Salmonella enterica serovar Brancaster (PS01) that was isolated from poultry meat in Malaysia. METHODS: Genomic DNA was extracted from Salmonella strain PS01 and was sequenced using an Illumina HiSeq 2000 platform. The generated reads were de novo assembled using CLC Genomics Workbench...
March 12, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28293235/distribution-of-cfr-in-staphylococcus-spp-and-escherichia-coli-strains-from-pig-farms-in-china-and-characterization-of-a-novel-cfr-carrying-f43-a-b-plasmid
#17
Xiao-Qin Liu, Jing Wang, Wei Li, Li-Qing Zhao, Yan Lu, Jian-Hua Liu, Zhen-Ling Zeng
The multi-resistance gene cfr is widely distributed among various gram-positive and gram-negative species in livestock in China. To better understand the epidemiology of cfr among Staphylococcus spp. and E. coli isolates, 254 Staphylococcus spp. and 398 E. coli strains collected from six swine farms in China were subjected to prevalence and genetic analysis. Forty (15.7%) Staphylococcus spp. isolates, including 38 Staphylococcus sciuri strains, one Staphylococcus chromogenes strain, and one Staphylococcus lentus strain, and two (0...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28288398/characterization-of-extended-spectrum-%C3%AE-lactamases-esbls-producing-salmonella-in-retail-raw-chicken-carcasses
#18
Jing Qiao, Qiang Zhang, Walid Q Alali, Jiawei Wang, Lingyuan Meng, Yingping Xiao, Hua Yang, Sheng Chen, Shenghui Cui, Baowei Yang
Extended-spectrum β-lactamases (ESBLs)-producing Salmonella is considered a serious concern to public health worldwide. However, limited information is available on ESBLs-producing Salmonella in retail chicken products in China. The objective of this study was to characterize ESBLs-producing Salmonella isolates from retail chickens in China. A total of 890 Salmonella isolates from retail chicken carcasses collected from 4 provinces were firstly screened for ESBLs-production phenotype via the double-disk synergy test method...
May 2, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28250772/molecular-characteristics-and-antibiotic-resistance-profiles-of-klebsiella-isolates-in-mthatha-eastern-cape-province-south-africa
#19
Sandeep Vasaikar, Larry Obi, Isaac Morobe, Mary Bisi-Johnson
The increase in the incidence of extended-spectrum β-lactamase- (ESBL-) producing Klebsiella species has become a serious problem worldwide, because of their incrimination in antibiotic resistance. The objective of this study is to investigate the resistance genes responsible for ESBL-producing Klebsiella species and carbapenemase-producing Klebsiella (CRE) isolated in Mthatha and to study their epidemiology. A prospective, descriptive study of 202 nonrepetitive samples from patients was obtained from Nelson Mandela Academic Hospital...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28243225/prevalence-and-molecular-characteristics-of-extended-spectrum-%C3%AE-lactamase-genes-in-escherichia-coli-isolated-from-diarrheic-patients-in-china
#20
Li Bai, Lili Wang, Xiaorong Yang, Juan Wang, Xin Gan, Wei Wang, Jin Xu, Qian Chen, Ruiting Lan, Séamus Fanning, Fengqin Li
Background: The emergence and spread of antimicrobial resistance has become a major global public health concern. A component element of this is the spread of the plasmid-encoded extended-spectrum b-lactamase (ESBL) genes, conferring resistance to third-generation cephalosporins. The purpose of this study was to investigate the molecular characteristics of ESBL-encoding genes identified in Escherichia coli cultured from diarrheic patients in China from 2013 to 2014. Materials and Methods: A total of 51 E. coli were confirmed as ESBL producers by double-disk synergy testing of 912 E...
2017: Frontiers in Microbiology
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