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https://www.readbyqxmd.com/read/29323530/prevalence-and-molecular-characterization-of-antimicrobial-resistance-in-escherichia-coli-isolated-from-raw-milk-and-raw-milk-cheese-in-egypt
#1
Rabee A Ombarak, Atsushi Hinenoya, Abdel-Rahman M Elbagory, Shinji Yamasaki
The goal of this study was to examine antimicrobial resistance and characterize the implicated genes in 222 isolates of Escherichia coli from 187 samples of raw milk and the two most popular cheeses in Egypt. E. coli isolates were tested for susceptibility to 12 antimicrobials by a disk diffusion method. Among the 222 E. coli isolates, 66 (29.7%) were resistant to one or more antimicrobials, and half of these resistant isolates showed a multidrug resistance phenotype (resistance to at least three different drug classes)...
January 11, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29311074/molecular-epidemiology-and-mechanism-of-sulbactam-resistance-in-acinetobacter-baumannii-isolates-with-diverse-genetic-background-in-china
#2
Yunxing Yang, Ying Fu, Peng Lan, Qingye Xu, Yan Jiang, Yan Chen, Zhi Ruan, Shujuan Ji, Xiaoting Hua, Yunsong Yu
Sulbactam is a plausible option for treating Acinetobacter infection because of its intrinsic antibacterial activity against the Acinetobacter genus, but the mechanisms of sulbactam resistance have not been studied fully in Acinetobacter baumannii In this study, a total of 2197 clinical A. baumannii isolates were collected from 27 provinces in China. Eighty-eight isolates with various minimal inhibitory concentrations (MICs) for sulbactam were selected based on their diverse clonality and underwent multilocus sequence typing, antimicrobial-susceptibility testing and resistance gene screening...
January 8, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29311067/molecular-epidemiology-of-emerging-blaoxa-23-like-and-blaoxa-24-like-carrying-acinetobacter-baumannii-in-taiwan
#3
Shu-Chen Kuo, Wei-Cheng Huang, Tzu-Wen Huang, Hui-Ying Wang, Jui-Fen Lai, Te-Li Chen, Tsai-Ling Lauderdale
Carbapenem-resistant Acinetobacter baumannii (CRAB) has increased significantly in recent decade in Taiwan. This study investigated the molecular epidemiology of CRAB with focus on the mechanisms of resistance and spread in isolates with blaOXA-23-like or blaOXA-24-like All 555 CRAB isolates in our 2002-2010 multi-centered collection were tested for the presence of class A, B and D carbapenemase genes. All isolates with blaOXA-23-like or blaOXA-24-like were subjected to PFGE and 82 isolates (60 blaOXA-23-like and 22 blaOXA-24-like) were selected for MLST to determine sequence type (ST) and clonal group (CG), and detection of additional β-lactamase and aminoglycoside genes...
January 8, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29310399/blaoxa-23-like-and-blatem-rather-than-blaoxa-51-like-contributed-to-a-high-level-of-carbapenem-resistance-in-acinetobacter-baumannii-strains-from-a-teaching-hospital-in-xi-an-china
#4
Lei Han, Jine Lei, Jiru Xu, Shaoshan Han
Acinetobacter baumannii is one of the major threats in clinical infections due to its antibiotic resistance ability. It shows increasing resistance to carbapenems, mainly due to β-lactamase mediated mechanisms. The aim of this study was to investigate carbapenem resistance (CR) profiles and analyze β-lactamases genes composition of clinical A. baumannii strains from a teaching hospital in Xi'an. The resistance patterns to imipenem and meropenem were checked for 51 clinical A. baumannii strains. The existence of 15 β-lactamases genes was detected by polymerase chain reaction (PCR), and the positive genes were sequenced...
December 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29290482/first-report-on-extended-spectrum-beta-lactamase-esbl-producing-escherichia-coli-from-european-free-tailed-bats-tadarida-teniotis-in-portugal-a-one-health-approach-of-a-hidden-contamination-problem
#5
Andreia Garcês, Susana Correia, Francisco Amorim, José Eduardo Pereira, Gilberto Igrejas, Patrícia Poeta
The main aim of this study was to characterize the diversity of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli isolates from European free tailed-bats (Tadarida teniotis) in Portugal. ESBL-producing E. coli isolates were recovered from 14 of 146 faecal samples (9.6%) and a total of 19 isolates were completely characterized. The more prevalent beta-lactamase genes detected were blaCTX-M-1 (57.9%) and blaCTX-M-3 (36.8%), followed by blaSHV (31.6%), blaTEM (21.1%), blaOXA (10.5%) and blaCTX-M-9 (10...
December 23, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29277529/comparative-genomics-of-the-rmtb-carrying-inci1-st136-plasmids-in-avian-escherichia-coli-isolates-from-chickens-in-china
#6
Li Yuan, Jian-Hua Liu, Xiang-Dang Du, Zhi-Yong Zong, Min Chen, Gong-Zheng Hu, Yu-Shan Pan
Eight rmtB-carrying avian Escherichia coli strains from a farm in China were characterized in our previous study, but little is known about the backbones and entire multiresistance regions (MRRs) of these plasmids. Here, three rmtB-carrying IncI1 ST136 plasmids were analyzed by whole-plasmid sequencing and compared. These plasmids were composed of an 83,470-bp IncI1 backbone carrying genes responsible for plasmid replication, transfer, maintenance, and stability functions, and a 17,330-bp MRR for pEC006 and pEC007, and a 34,626-bp MRR for pEC008...
December 22, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29274456/molecular-characterization-of-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-strains-isolated-from-a-tertiary-care-hospital
#7
Jayanti Jena, Nagen Kumar Debata, Rajesh Kumar Sahoo, Mahendra Gaur, Enketeswara Subudhi
Infectious diseases caused by ESBL producing Enterobacteriaceae are an emerging problem worldwide which increases the empirical treatment failure, hospital cost, rate of morbidity and mortality. This also leads to the Hospital infection outbreak. Present study was undertaken to determine the frequency of blaTEM, blaCTX-M and blaSHV genes among Enterobacteriaceae. A total of 751 non-repeated clinical isolates of Enterobacteriaceae family were included in this study. Antibiotic susceptibility test was done and minimal inhibitory concentration (MIC) against four antibiotics was carried out...
December 20, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29234497/insertional-inactivation-of-oprd-in-carbapenem-resistant-pseudomonas-aeruginosa-strains-isolated-from-burn-patients-in-tehran-iran
#8
A Shariati, T Azimi, A Ardebili, A S Chirani, A Bahramian, A Pormohammad, M Sadredinamin, S Erfanimanesh, N Bostanghadiri, S Shams, A Hashemi
In this study, we report the insertion sequence ISPpu21 in the oprD porin gene of carbapenem-resistant Pseudomonas aeruginosa isolates from burn patients in Tehran, Iran. Antibiotic susceptibility tests for P. aeruginosa isolates were determined. Production of metallo-β-lactamases (MBLs) and carbapenemase was evaluated and the β-lactamase-encoding and aminoglycoside-modifying enzyme genes were investigated by PCR and sequencing methods. The mRNA transcription level of oprD and mex efflux pump genes were evaluated by real-time PCR...
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29233495/antibacterial-resistance-patterns-of-extended-spectrum-%C3%AE-lactamase-producing-enteropathogenic-escherichia-coli-strains-isolated-from-children
#9
Pejman Karami, Hassan Bazmamoun, Iraj Sedighi, Amir Sasan Mozaffari Nejad, Mohammad Mehdi Aslani, Mohammad Yousef Alikhani
BACKGROUND AND STUDY AIM: This study aimed to determine the antibacterial resistance patterns of extended spectrum β-lactamase (ESBL)-producing enteropathogenic Escherichia coli (EPEC) isolated from Iranian children and to investigate its genetic patterns. PATIENTS AND METHODS: 192 non-repeats EPEC isolates were collected from stool samples of the children with and without diarrhoea. The EPEC strains were isolated from 1355 stool specimens obtained from 247 children with diarrhoea (0-10 years old; mean age, 5...
December 7, 2017: Arab Journal of Gastroenterology: the Official Publication of the Pan-Arab Association of Gastroenterology
https://www.readbyqxmd.com/read/29227182/first-report-of-the-plasmid-mediated-colistin-resistance-gene-mcr-1-in-escherichia-coli-st405-isolated-from-wildlife-in-bejaia-algeria
#10
Taous Bachiri, Rym Lalaoui, Sofiane Bakour, Meriem Allouache, Nadia Belkebla, Jean Marc Rolain, Abdelaziz Touati
BACKGROUND: The aim of the present study was to screen for the presence of mcr-1 gene in Enterobacteriaceae isolated from Barbary macaques (Macaca sylvanus) in Algeria. MATERIALS AND METHODS: From January to April 2016, a total of 86 fresh stool samples from Barbary macaques were collected in the Toudja forest (Bejaia, Algeria). After isolation, the isolates were identified by matrix-assisted laser desorption and ionization time-of-flight mass spectrometry. Antibiotic susceptibility was determined by disk diffusion method, and minimum inhibitory concentration (MIC) of colistin was determined by E-test...
December 11, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29213998/phenotypic-and-genetic-characterization-of-%C3%AE-lactam-resistance-in-klebsiella-from-retail-chicken-meat-in-mansoura-egypt
#11
Hazem Ramadan, Amal Awad
Background and Objectives: This study was undertaken to characterize antimicrobial resistance phenotypes and genes encoding extended spectrum β-lactamases (ESBLs) in Klebsiella isolated from retail chicken meat in Mansoura, Egypt. Materials and Methods: Three hundred sixty chicken meat samples from 120 eviscerated chicken carcasses (3 cuts each) collected randomly from local retail chicken shops in Mansoura, Egypt during the period from April to June 2015, were assayed for the presence of Klebsiella by conventional bacteriological methods...
April 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29201017/characterization-of-metagenomes-in-urban-aquatic-compartments-reveals-high-prevalence-of-clinically-relevant-antibiotic-resistance-genes-in-wastewaters
#12
Charmaine Ng, Martin Tay, Boonfei Tan, Thai-Hoang Le, Laurence Haller, Hongjie Chen, Tse H Koh, Timothy M S Barkham, Karina Y-H Gin
The dissemination of antimicrobial resistance (AMR) is an escalating problem and a threat to public health. Comparative metagenomics was used to investigate the occurrence of antibiotic resistant genes (ARGs) in wastewater and urban surface water environments in Singapore. Hospital and municipal wastewater (n = 6) were found to have higher diversity and average abundance of ARGs (303 ARG subtypes, 197,816 x/Gb) compared to treated wastewater effluent (n = 2, 58 ARG subtypes, 2,692 x/Gb) and surface water (n = 5, 35 subtypes, 7,985 x/Gb)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29198740/early-transmissible-ampicillin-resistance-in-zoonotic-salmonella-enterica-serotype-typhimurium-in-the-late-1950s-a-retrospective-whole-genome-sequencing-study
#13
Alicia Tran-Dien, Simon Le Hello, Christiane Bouchier, François-Xavier Weill
BACKGROUND: Ampicillin, the first semi-synthetic penicillin active against Enterobacteriaceae, was released onto the market in 1961. The first outbreaks of disease caused by ampicillin-resistant strains of Salmonella enterica serotype Typhimurium were identified in the UK in 1962 and 1964. We aimed to date the emergence of this resistance in historical isolates of S enterica serotype Typhimurium. METHODS: In this retrospective, whole-genome sequencing study, we analysed 288 S enterica serotype Typhimurium isolates collected between 1911 and 1969 from 31 countries on four continents and from various sources including human beings, animals, feed, and food...
November 29, 2017: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/29185915/antibiotic-resistance-gene-detection-in-the-microbiome-context
#14
Thi Thuy Do, Javier Tamames, Robert D Stedtfeld, Xueping Guo, Sinead Murphy, James M Tiedje, Fiona Walsh
Within the past decade, microbiologists have moved from detecting single antibiotic resistance genes (ARGs) to detecting all known resistance genes within a sample due to advances in next generation sequencing. This has provided a wealth of data on the variation and relative abundances of ARGs present in a total bacterial population. However, to use these data in terms of therapy or risk to patients, they must be analyzed in the context of the background microbiome. Using a quantitative PCR ARG chip and 16S rRNA amplicon sequencing, we have sought to identify the ARGs and bacteria present in a fecal sample of a healthy adult using genomic tools...
November 29, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29184377/virulence-and-extended-spectrum-%C3%AE-lactamase-encoding-genes-in-escherichia-coli-recovered-from-chicken-meat-intended-for-hospitalized-human-consumption
#15
Gamal A Younis, Rasha M Elkenany, Mohamed A Fouda, Noura F Mostafa
Aim: This study describes the prevalence of Escherichia coli in frozen chicken meat intended for human consumption with emphasis on their virulence determinants through detection of the virulence genes and recognition of the extended-spectrum β-lactamase (ESBL) encoding genes (blaOXA and blaTEM genes). Materials and Methods: A total of 120 frozen chicken meat samples were investigated for isolation of E. coli. All isolates were subjected to biochemical and serological tests...
October 2017: Veterinary World
https://www.readbyqxmd.com/read/29184370/occurrence-of-virulent-and-antibiotic-resistant-shiga-toxin-producing-escherichia-coli-in-some-food-products-and-human-stool-in-egypt
#16
Osman Mohamed Hamed, Maha Ahmed Sabry, Nawal A Hassanain, Eman Hamza, Ahmed G Hegazi, Marwa Badawy Salman
Aim: Shiga toxin-producing Escherichia coli (STEC) represent a severe public health issue worldwide, causing life-threatening diseases in the human gastrointestinal tract. This study aimed to determine the occurrence of virulent and antibiotic-resistant STEC in retail meat and milk products and human stool samples and to characterize the genes encoding for virulence and antibiotic resistance among the identified STEC isolates. Materials and Methods: A total of 260 food samples were randomly collected from retail markets in different localities of El Giza Governorate, Egypt...
October 2017: Veterinary World
https://www.readbyqxmd.com/read/29184361/phenotypic-and-genotypic-characterization-of-antimicrobial-susceptibility-of-avian-pathogenic-escherichia-coli-isolated-from-broiler-chickens
#17
Gamal Younis, Amal Awad, Nada Mohamed
Aim: Avian pathogenic Escherichia coli (APEC) is pathogenic strains of E. coli that are responsible for one of the most common bacterial diseases affecting poultry worldwide. This study was designed to determine the occurrence, antibiotic resistance profile, and antibiotic resistance genes of E. coli isolated from diseased and freshly dead broilers. Materials and Methods: In that context, a total of 200 broilers samples were examined by standard microbiological techniques for isolation of E...
October 2017: Veterinary World
https://www.readbyqxmd.com/read/29180282/antibiotic-resistance-genes-in-phage-particles-isolated-from-human-feces-and-induced-from-clinical-bacterial-isolates
#18
Maryury Brown-Jaque, William Calero-Cáceres, Paula Espinal, Judith Rodríguez-Navarro, Elisenda Miró, Juan José González-López, Thais Cornejo, Juan Carlos Hurtado, Ferran Navarro, Maite Muniesa
Phage particles have emerged as elements with the potential to mobilize antibiotic resistance genes (ARGs) in different environments, including the intestinal habitat. The aim of this study was to determine the occurrence of ARGs in phage particles present in fecal matter and induced from strains isolated from feces. Nine ARGs (blaTEM, blaCTX-M-1-group, blaCTX-M-9-group, blaOXA-48, qnrA, qnrS, mecA, sul1 and armA) were quantified by qPCR in the phage DNA fractions of 150 fecal samples obtained from healthy individuals...
November 24, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29169020/biomarkers-of-antibiotic-resistance-genes-during-seasonal-changes-in-wastewater-treatment-systems
#19
Ya-Nan Jiao, Zhen-Chao Zhou, Tao Chen, Yuan-Yuan Wei, Ji Zheng, Rui-Xia Gao, Hong Chen
To evaluate the seasonal distribution of antibiotic resistance genes (ARGs) and explore the reason for their patterns in different seasons and different systems, two wastewater treatment systems were selected and analyzed using high-throughput qPCR. Linear discriminant analysis (LDA) effect size (LEfSe) was used to discover the differential ARGs (biomarkers) and estimate the biomarkers' effect size. We found that the total absolute abundances of ARGs in inflows and excess sludge samples had no obvious seasonal fluctuations, while those in winter outflow samples decreased in comparison with the inflow samples...
November 20, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29163400/prevalence-and-antibiotic-resistance-of-non-typhoidal-salmonella-isolated-from-raw-chicken-carcasses-of-commercial-broilers-and-spent-hens-in-tai-an-china
#20
Song Li, Yufa Zhou, Zengmin Miao
The present study was aimed to determine the prevalence and characteristics of Salmonella isolated from meat samples of commercial broilers (CB) and spent hens (SH). Between March and June 2016, 200 retail raw chicken carcasses (100 from CB and 100 from SH) were obtained from local supermarkets in Tai'an city of China, and Salmonella isolates were then analyzed for antibiotic resistance, serotype, β-lactamase genes, and the presence of class 1 integron. Forty Salmonella strains were obtained in this study (CB: 21/100, 21%; SH: 19/100, 19%)...
2017: Frontiers in Microbiology
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