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Sinem Acar, Ece Bulut, Bora Durul, Ilhan Uner, Mehmet Kur, M Dilek Avsaroglu, Hüseyin Avni Kirmaci, Yasar Osman Tel, Fadile Y Zeyrek, Yesim Soyer
192 Food samples (commonly consumed 8 food types), 355 animal samples (animal feces of bovine, ovine, goat and chicken) and 50 samples from clinical human cases in Sanliurfa city, Turkey in a year were collected to determine the Salmonella enterica subsp. enterica mosaic in Turkey. 161 Salmonella isolates represented 17 serotypes, 20 sequence types (STs) and 44 PFGE patterns (PTs). 3 serotypes, S. Enteritidis, S. Typhimurium and S. Kentucky, were recovered from three different hosts. The highest discriminatory power was obtained by PFGE (SID=0...
October 8, 2016: International Journal of Food Microbiology
Rui Seixas, Tânia Raquel Santos, Jorge Machado, Luís Tavares, Fernando Bernardo, Teresa Semedo-Lemsaddek, Manuela Oliveira
The increase in prevalence of Salmonella 1,4,[5],12:i:- related infections over the last few years has been considered a public health issue in many European countries, especially as this serovar may be associated with tetraresistance to ampicillin, streptomycin, sulfonamides, and tetracyclines (R-type ASSuT). Salmonella 1,4,[5],12:i:- isolates (n = 187) obtained by the Portuguese National Laboratory from different sources, including human clinical cases (n = 170), veterinary (n = 10), environmental (n = 6), and food samples (n = 1), were collected from 15 districts between 2006 and 2011...
October 21, 2016: Foodborne Pathogens and Disease
G Odewale, O J Adefioye, J Ojo, F A Adewumi, O A Olowe
Acinetobacter baumannii is a ubiquitous pathogen that has emerged as a major cause of healthcare-associated infections at Ladoke Akintola University Teaching Hospital. Isolates were assayed according to standard protocol. The isolates were subjected to molecular techniques to detect blaOXA, blaTEM, blaCTX-M, and blaSHV genes in strains of the A. baumannii isolates. The prevalence of A. baumannii was 8.5% and was most prevalent among patients in the age group 51-60 (36%); the male patients (63.6%) were more infected than their female counterparts...
September 29, 2016: European Journal of Microbiology & Immunology
Ana Flores-Carrero, Indira Labrador, Alberto Paniz-Mondolfi, David R Peaper, Dana Towle, María Araque
Enterobacter spp. have emerged as an important group of pathogens linked to outbreaks in neonatal intensive care units (NICUs), usually involving strains expressing extended-spectrum β-lactamases (ESBLs). The aim of this study was to describe the first nosocomial bloodstream infection outbreak caused by Enterobacter ludwigii co-harbouring CTX-M-8, SHV-12 and TEM-15 in a NICU in a Venezuelan hospital. Initial bacterial identification was achieved by VITEK(®)2 system and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (VITEK(®) MS) and was subsequently confirmed by nucleotide sequencing of the 16S rDNA gene and hsp60 genotyping...
October 7, 2016: Journal of Global Antimicrobial Resistance
Wenyao Chen, Tingzi Fang, Xiujuan Zhou, Daofeng Zhang, Xianming Shi, Chunlei Shi
The wide usage of antibiotics contributes to the increase in the prevalence of antibiotic-resistant Salmonella. Plasmids play a critical role in horizontal transfer of antibiotic resistance markers in Salmonella. This study aimed to screen and characterize plasmid profiles responsible for antibiotic resistance in Salmonella and ultimately to clarify the molecular mechanism of transferable plasmid-mediated antibiotic resistance. A total of 226 Salmonella isolates were examined for antimicrobial susceptibility by a disk diffusion method...
2016: Frontiers in Microbiology
Bhoomika, Sanjay Shakya, Anil Patyal, Nitin Eknath Gade
AIM: To assess the prevalence of antimicrobial resistance producing extended-spectrum β-lactamases (ESBL) (blaTEM, blaSHV, and blaCTX-M) genes in Escherichia coli isolated from chicken meat, chevon meat, raw milk, and human urine and stool samples collected from tribal districts of Chhattisgarh, viz., Jagdalpur, Dantewada, Kondagaon, and Kanker. MATERIALS AND METHODS: A total of 330 samples, comprising 98 chicken meat, 82 chevon meat, 90 raw milk, and 60 human urine and stool samples, were processed for isolation of E...
September 2016: Veterinary World
Jing Yu, Kun Tan, Zhihui Rong, Yue Wang, Zhongju Chen, Xuhui Zhu, Li Wu, Li Tan, Wei Xiong, Ziyong Sun, Ling Chen
BACKGROUND: The spread of resistance to carbapenems among Enterobacteriaceae has become a major public health problem in recent years. In this study, we describe an outbreak of Klebsiella pneumoniae in the neonatal ward. First, we aimed to study the drug resistance, genetic relatedness, and transmission mechanism of carbapenem-resistant K. pneumoniae; second, we implemented infection control measures to contain the outbreak. METHODS: We investigated 27 non-repetitive strains isolated from neonates and five strains cultured from around the neonatal ward...
October 12, 2016: BMC Infectious Diseases
Maryam Haghighatpanah, Amir Sasan Mozaffari Nejad, Ali Mojtahedi, Nour Amirmozafari, Habib Zeighami
Escherichia coli is an important cause of hospital-acquired infections worldwide. Antimicrobial resistance leads to treatment failure of hospital infections caused by E. coli. Production of extended-spectrum β-lactamases (ESBLs) is one of the major causes of antibiotic resistance in these bacteria. This study aimed to investigate the frequency of blaTEM and blaCTX-M genes in ESBL-producing E. coli strains isolated from clinical specimens of patients admitted to six hospitals in the north of Iran. A total of 160 E...
October 1, 2016: Journal of Global Antimicrobial Resistance
Ming-Yi Wang, Jian-Li Geng, Ying-Jian Chen, Yu Song, Mei Sun, Hai-Zhu Liu, Cheng-Jin Hu
Methicillin-resistant staphylococci and ESBL-producing bacteria are the most important resistant pathogens in sepsis. In the study, a new multiplex-touchdown PCR method (MT-PCR) was developed to detect rapidly and simultaneously the presence of mecA, blaSHV , blaCTX-M , blaTEM and blaOXA genes from positive blood culture bottles. The technique showed a sensitivity of 10(3) CFU ml(-1) for mecA detection and of 10(2) CFU ml(-1) for other genes, and 100% specificity in the detection of all genes. All genes were detected in the spiked blood culture bottles artificially contaminated with reference strains...
October 4, 2016: Letters in Applied Microbiology
Carlos Narciso-da-Rocha, Célia M Manaia
The aim of this study was to assess the fate of invasive antibiotic resistance genes (ARG) discharged in wastewater. With this objective, antibiotic resistant bacteria (ARB) known to harbor specific ARG were inoculated in wastewater (hospital effluent, or municipal raw and treated wastewater) and in ultra-pure sterile water microcosms. Two sets of wastewater ARB isolates were used - set 1, Enterococcus faecalis, Acinetobacter johnsonii, Klebsiella pneumoniae and set 2, Enterococcus faecium, Acinetobacter johnsonii, Escherichia coli...
September 30, 2016: Science of the Total Environment
Qi Ni, Yuan Tian, Lihua Zhang, Cen Jiang, Danfeng Dong, Zhen Li, Enqiang Mao, Yibing Peng
OBJECTIVES: To characterize extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates from the community, determine their antibiotic sensitivity profiles and quinolone resistance mechanisms, and identify any horizontal transfer of ESBL genes. METHODS: One thousand seven hundred thirty-two stool samples were collected from healthy individuals in 6 communities and 2 physical examination centers in Shanghai, China. ESBL-producing E. coli was screened and confirmed by confirmatory test and E...
July 12, 2016: Diagnostic Microbiology and Infectious Disease
Lotfi Loucif, Ahmed Kassah Laouar, Mahdia Saidi, Amina Messala, Widad Chelaghma, Jean-Marc Rolain
Seven non-redundant ertapenem-resistant Klebsiella pneumoniae isolates were collected from May 2014 to January 19, 2015 in the nephrology and hematology units of Batna University Hospital, Algeria. All strains coproduced the blaOXA-48, blaCTX-M-15, blaSHV-1 and blaTEM-1D genes. Six of these isolates belonged to the pandemic clone ST101. The blaOXA-48 gene was located on a conjugative IncL/M-type plasmid. This is the first outbreak of OXA-48-producing K. pneumoniae isolates involving an ST101 clone in Batna University Hospital, Algeria...
September 19, 2016: Antimicrobial Agents and Chemotherapy
M A Gonggrijp, I M G A Santman-Berends, A E Heuvelink, G J Buter, G van Schaik, J J Hage, T J G M Lam
A cross-sectional study was conducted to evaluate the prevalence of extended-spectrum β-lactamase (ESBL)- and plasmid-mediated AmpC-producing Escherichia coli and associated risk factors in dairy herds. One hundred dairy herds were randomly selected and sampled to study the presence of ESBL- and AmpC-producing E. coli in slurry samples. The sensitivity of testing slurry samples for ESBL/AmpC herd status is less than 100%, especially for detecting herds with a low ESBL/AmpC prevalence. Therefore, whereas herds that tested positive for ESBL/AmpC-producing E...
September 13, 2016: Journal of Dairy Science
Geovana Brenner Michael, Heike Kaspar, Amanda Keller Siqueira, Eduardo de Freitas Costa, Luís Gustavo Corbellini, Kristina Kadlec, Stefan Schwarz
The aim of this study was to identify extended-spectrum β-lactamase (ESBL)-producing Escherichia coli collected from diseased food-producing animals in Germany. A total of 6849 E. coli isolates, collected from diseased cattle, pigs and poultry in the German national monitoring program GERM-Vet (2008-2014), were characterized by antimicrobial susceptibility testing and screened for the ESBL phenotype. ESBL genes were identified by PCR and sequencing. The isolates were further characterized by PCR-based phylotyping...
September 1, 2016: Veterinary Microbiology
María José Grande Burgos, Maria Luisa Fernández Márquez, Rubén Pérez Pulido, Antonio Gálvez, Rosario Lucas López
Eggs may contain extraintestinal pathogenic (ExPEC) and diarrheogenic (DEC) Escherichia coli which in addition may carry antibiotic resistance. The wide use of biocides and disinfectants in the food industry may induce biocide tolerance in bacteria. The aim of the present study was to evaluate biocide tolerance and antibiotic resistance in E. coli from hen egg shells. A total of 27 isolates obtained from a screening of 180 eggs were studied. Seven isolates carried both eae and bfpA genes of typical enteropathogenic E...
December 5, 2016: International Journal of Food Microbiology
Mónika Szabó, Tibor Nagy, Tímea Wilk, Tibor Farkas, Anna Hegyi, Ferenc Olasz, János Kiss
Two IncA/C family plasmids from the 1960s have been sequenced and classified into A/C2 Type 1 group. R16a and IP40a contain novel antibiotic resistance islands and a complete GIsul2 island not previously found in the family. In the 173.1 kb R16a the 29.9 kb ARI is located in a unique backbone position not utilized by ARIs. ARIR16a consists of Tn1, Tn6020, Tn6333 harboring the resistance genes blaTEM-1D, aphA1b and a mer module, respectively, a truncated Tn5393 copy and a gene cluster with unknown function. Plasmid IP40a is 170...
September 6, 2016: Antimicrobial Agents and Chemotherapy
Songsak Srisanga, Sunpetch Angkititrakul, Patcharee Sringam, Phuong T Le Ho, An T T Vo, Rungtip Chuanchuen
A total of 122 Salmonella isolates from dogs (n=113) and cats (n=9) comprising 32 serotypes were obtained from household dogs (n=250) and cats (n=50) during 2012-2015. The isolates were characterized by serotyping, examining phenotype and genotype of antimicrobial resistance and screening virulence genes. Serovars Weltevreden (15.6%) and Typhimurium (13.9%) were the most common serotypes. The majority of the isolates were multidrug resistant (43%). The dog isolates (12.3%) harbored class 1 integrons, of which the dfrA12-aadA2 cassette was most frequently identified (66...
September 1, 2016: Journal of Veterinary Science
Fabianne Carlesse, Paola Cappellano, Milene Gonçalves Quiles, Liana Carballo Menezes, Antonio Sérgio Petrilli, Antonio Carlos Pignatari
BACKGROUND: Bloodstream infections (BSIs) are the major cause of mortality in cancer patients. Molecular techniques are used for rapid diagnosis of BSI, allowing early therapy and improving survival. We aimed to establish whether real-time quantitative polymerase chain reaction (qPCR) could improve early diagnosis and therapy in paediatric cancer patients, and describe the predominant pathogens of BSI and their antimicrobial susceptibility. METHODS: Blood samples were processed by the BACTEC system and microbial identification and susceptibility tests were performed by the Phoenix system...
2016: BMC Infectious Diseases
Chanwit Tribuddharat, Pintip Pongpech, Angkana Charoenwatanachokchai, Somchai Lokpichart, Somporn Srifuengfung, Suthatta Sonprasert
We studied antimicrobial susceptibility and the current rates of blaTEM-1 and blaTEM-135. Neisseria gonorrhoeae 370 isolates were tested by using disk diffusion method and found 100% susceptible to cefixime, ceftriaxone, cefotaxime, ceftazidime, cefepime, spectinomycin and azithromycin. They were resistant to penicillin (85.7%), ciprofloxacin (88.0%), ofloxacin (97.4%), and tetracycline (89.1%). Penicillinase-producing N. gonorrhoeae (PPNG) was 83.8% which 70.0% was further identified as PPNG plus tetracycline resistant N...
August 31, 2016: Japanese Journal of Infectious Diseases
Satomi Saito, Yoshio Koori, Yusuke Ohsaki, Shunsuke Osaka, Kozue Oana, Yukiko Nagano, Yoshichika Arakawa, Noriyuki Nagano
Detection and characterization of β-lactamase genes in a total of ten non-typhoidal Salmonella (NTS) clinical isolates exhibiting resistance to the third-generation cephalosporins collected during 2012 -2014 in Japan were conducted. Among five strains with cefotaxime MIC (minimum inhibitory concentration) ≥64 μg/ml and positive results of clavulanic acid inhibition effect, blaCTX-M-2 was detected in three strains (serotypes Stanley and Muenchen), and each of blaTEM-52 (serotype Manhattan) and blaSHV-12 (serotype Infantis) was found in one strain...
August 31, 2016: Japanese Journal of Infectious Diseases
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